WO2011108916A3 - A method for recovering an intracellular polyhydroxyalkanoate (pha) - Google Patents

A method for recovering an intracellular polyhydroxyalkanoate (pha) Download PDF

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Publication number
WO2011108916A3
WO2011108916A3 PCT/MY2010/000333 MY2010000333W WO2011108916A3 WO 2011108916 A3 WO2011108916 A3 WO 2011108916A3 MY 2010000333 W MY2010000333 W MY 2010000333W WO 2011108916 A3 WO2011108916 A3 WO 2011108916A3
Authority
WO
WIPO (PCT)
Prior art keywords
pha
pellet
freeze
distilled water
recovering
Prior art date
Application number
PCT/MY2010/000333
Other languages
French (fr)
Other versions
WO2011108916A2 (en
Inventor
Mohd Ali Hassan
Mitra Mohammadi
Nor Asma Ab Razak
Mei Ling Chong
Suraini Abd Aziz
Amirul Al-Ashraf Abdullah
Hasfalina Che Man
Siti Nor Syairah Anis
Lai Yee Phang
Yoshihito Shirai
Wan Md Zin Wan Yunus
Original Assignee
Universiti Putra Malaysia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiti Putra Malaysia filed Critical Universiti Putra Malaysia
Publication of WO2011108916A2 publication Critical patent/WO2011108916A2/en
Publication of WO2011108916A3 publication Critical patent/WO2011108916A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
    • C08G63/88—Post-polymerisation treatment
    • C08G63/89—Recovery of the polymer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a method to recover an intracellular polyhydroxyalkanoates (PHA). The method includes the steps of (a) separating culture medium containing PHA-rich cells using centrifugation, (b) rinsing pellet obtained from step (a) with distilled water, (c) freeze- drying the pellet obtained in step (b), (d) adding distilled water to the freeze-dried sample, (e) separating non-PHA cellular material (NPCM) from PHA granules, (f) washing the pellet containing PHA from step (e) by distilled water and centrifuging for producing final pellet, and (g) freeze-drying the pellet produced from step (f).
PCT/MY2010/000333 2010-03-01 2010-12-30 A method for recovering an intracellular polyhydroxyalkanoate (pha) WO2011108916A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2010000891A MY153891A (en) 2010-03-01 2010-03-01 A method for recovering an intracellular polyhydroxyalkanoate (pha)
MYPI2010000891 2010-03-01

Publications (2)

Publication Number Publication Date
WO2011108916A2 WO2011108916A2 (en) 2011-09-09
WO2011108916A3 true WO2011108916A3 (en) 2011-11-24

Family

ID=44542763

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MY2010/000333 WO2011108916A2 (en) 2010-03-01 2010-12-30 A method for recovering an intracellular polyhydroxyalkanoate (pha)

Country Status (2)

Country Link
MY (1) MY153891A (en)
WO (1) WO2011108916A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107429274A (en) 2014-11-20 2017-12-01 全循环生物塑料有限公司 Polyhydroxy alkanoate copolymer is produced from debirs

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020031812A1 (en) * 2000-09-13 2002-03-14 La Societe Novartem Inc. Process for production of biopolymer
US7070966B2 (en) * 2000-03-14 2006-07-04 Ufz Umweltforschungszentrum Leipzig-Halle Gmbh Method for obtaining polyhydroxyalkanoates (PHA) and the copolymers thereof
US20070072276A1 (en) * 2003-12-19 2007-03-29 Xuejun Chen Method for separating, extracting and purifying poly-beta-hydroxyalkanoates (phas) directly from bacterial fermentation broth
US20080118963A1 (en) * 2002-09-30 2008-05-22 Noriko Ogawa Method Of Coagulating Poly-3-Hydroxyalkanoic Acid
US7393668B2 (en) * 2003-01-20 2008-07-01 Kaneka Corporation Method of collecting highly pure polyhydroxyalkanoate from microbial cells
US7435567B2 (en) * 2004-03-04 2008-10-14 Kaneka Corporation Method for degradation of nucleic acids and use thereof
WO2010071399A1 (en) * 2008-12-18 2010-06-24 Universiti Putra Malaysia A method of extracting and purifying polyhydroxy alkanoate bioplastic

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7070966B2 (en) * 2000-03-14 2006-07-04 Ufz Umweltforschungszentrum Leipzig-Halle Gmbh Method for obtaining polyhydroxyalkanoates (PHA) and the copolymers thereof
US20020031812A1 (en) * 2000-09-13 2002-03-14 La Societe Novartem Inc. Process for production of biopolymer
US20080118963A1 (en) * 2002-09-30 2008-05-22 Noriko Ogawa Method Of Coagulating Poly-3-Hydroxyalkanoic Acid
US7393668B2 (en) * 2003-01-20 2008-07-01 Kaneka Corporation Method of collecting highly pure polyhydroxyalkanoate from microbial cells
US20070072276A1 (en) * 2003-12-19 2007-03-29 Xuejun Chen Method for separating, extracting and purifying poly-beta-hydroxyalkanoates (phas) directly from bacterial fermentation broth
US7435567B2 (en) * 2004-03-04 2008-10-14 Kaneka Corporation Method for degradation of nucleic acids and use thereof
WO2010071399A1 (en) * 2008-12-18 2010-06-24 Universiti Putra Malaysia A method of extracting and purifying polyhydroxy alkanoate bioplastic

Also Published As

Publication number Publication date
WO2011108916A2 (en) 2011-09-09
MY153891A (en) 2015-04-15

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