WO2012124941A3 - Recombinant microorganism having ability to produce polyhydroxyalkanoate containing 2-hydroxybutyrate as monomer, and method for preparing polyhydroxyalkanoate containing 2-hydroxybutyrate as monomer using same - Google Patents

Recombinant microorganism having ability to produce polyhydroxyalkanoate containing 2-hydroxybutyrate as monomer, and method for preparing polyhydroxyalkanoate containing 2-hydroxybutyrate as monomer using same Download PDF

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Publication number
WO2012124941A3
WO2012124941A3 PCT/KR2012/001752 KR2012001752W WO2012124941A3 WO 2012124941 A3 WO2012124941 A3 WO 2012124941A3 KR 2012001752 W KR2012001752 W KR 2012001752W WO 2012124941 A3 WO2012124941 A3 WO 2012124941A3
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WO
WIPO (PCT)
Prior art keywords
hydroxybutyrate
monomer
polyhydroxyalkanoate containing
recombinant microorganism
ability
Prior art date
Application number
PCT/KR2012/001752
Other languages
French (fr)
Korean (ko)
Other versions
WO2012124941A2 (en
WO2012124941A9 (en
Inventor
이상엽
박시재
이승환
송봉근
이태우
Original Assignee
한국과학기술원
한국화학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국과학기술원, 한국화학연구원 filed Critical 한국과학기술원
Publication of WO2012124941A2 publication Critical patent/WO2012124941A2/en
Publication of WO2012124941A3 publication Critical patent/WO2012124941A3/en
Publication of WO2012124941A9 publication Critical patent/WO2012124941A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Biological Depolymerization Polymers (AREA)
  • Polyesters Or Polycarbonates (AREA)

Abstract

The present invention relates to a polyhydroxyalkanoate containing a 2-hydroxybutyrate as a monomer, a recombinant microorganism having the ability to produce the polyhydroxyalkanoate containing the 2-hydroxybutyrate as the monomer, and a method for preparing the polyhydroxyalkanoate containing the 2-hydroxybutyrate as the monomer. The present invention can effectively provide the polyhydroxyalkanoate containing the 2-hydroxybutyrate, which is a novel biodegradable polymer, and the recombinant microorganism for producing same.
PCT/KR2012/001752 2011-03-11 2012-03-09 Recombinant microorganism having ability to produce polyhydroxyalkanoate containing 2-hydroxybutyrate as monomer, and method for preparing polyhydroxyalkanoate containing 2-hydroxybutyrate as monomer using same WO2012124941A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0022021 2011-03-11
KR1020110022021A KR101328567B1 (en) 2011-03-11 2011-03-11 Recombinant Microorganism Producing polyhydroxyalkanoate (PHA) containing 2-hydroxybutyrate (2HB) monomer and Preparing Method of PHA containing 2HB Using Thereof

Publications (3)

Publication Number Publication Date
WO2012124941A2 WO2012124941A2 (en) 2012-09-20
WO2012124941A3 true WO2012124941A3 (en) 2012-12-27
WO2012124941A9 WO2012124941A9 (en) 2013-02-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/001752 WO2012124941A2 (en) 2011-03-11 2012-03-09 Recombinant microorganism having ability to produce polyhydroxyalkanoate containing 2-hydroxybutyrate as monomer, and method for preparing polyhydroxyalkanoate containing 2-hydroxybutyrate as monomer using same

Country Status (2)

Country Link
KR (1) KR101328567B1 (en)
WO (1) WO2012124941A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101630003B1 (en) * 2011-11-28 2016-06-13 주식회사 엘지화학 Method of preparing for 2-hydroxyalkanoate polymer
KR102088502B1 (en) * 2015-09-03 2020-03-12 주식회사 엘지화학 Copolymer comprising 4-hydroxybutyrate, 3-hydroxybutyrate, 2-hydroxybutyrate and lactate as repeating unit and method for preparing the same
KR102088503B1 (en) * 2015-09-03 2020-03-12 주식회사 엘지화학 Copolymer comprising 4-hydroxybutyrate, 3-hydroxybutyrate and 2-hydroxybutyrate as repeating unit and method for preparing the same
KR102088504B1 (en) * 2015-09-03 2020-03-12 주식회사 엘지화학 Copolymer comprising 3-hydroxypropionate, 2-hydroxybutyrate and lactate as repeating unit and method for preparing the same
KR102361618B1 (en) * 2018-01-08 2022-02-09 주식회사 엘지화학 Copolymer comprising 2-hydroxybutyrate and 3-hydroxybutyrate and method of preparing the same
KR102497785B1 (en) * 2018-01-08 2023-02-07 주식회사 엘지화학 Recombinant microorganism and method for preparing copolymer comprising 2-hydroxybutyrate and lactate
KR20190090602A (en) * 2018-01-25 2019-08-02 주식회사 엘지화학 Recombinant microorganism and method for preparing terpolymer comprising 2-hydroxybutyrate, 2-hydroxyisovalerate and lactate
CN115851510B (en) * 2022-10-13 2023-09-12 深圳中科翎碳生物科技有限公司 Salmonella and application thereof in co-production of tetrahydropyrimidine and polyhydroxyalkanoate

Citations (3)

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Publication number Priority date Publication date Assignee Title
US20080275208A1 (en) * 1998-05-22 2008-11-06 Metabolix, Inc. Delaware Polyhydroxyalkanoate biopolymer compositions
KR20090078925A (en) * 2008-01-16 2009-07-21 주식회사 엘지화학 Recombinant microorganism having a producing ability of polylactate or its copolymers and method for preparing polylactate or its copolymers using the same
KR20100090019A (en) * 2009-02-05 2010-08-13 주식회사 엘지화학 Recombinant ralstonia eutropha having an producing ability of polylactate or its copolymers and method for preparing polylactate or its copolymers using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080275208A1 (en) * 1998-05-22 2008-11-06 Metabolix, Inc. Delaware Polyhydroxyalkanoate biopolymer compositions
KR20090078925A (en) * 2008-01-16 2009-07-21 주식회사 엘지화학 Recombinant microorganism having a producing ability of polylactate or its copolymers and method for preparing polylactate or its copolymers using the same
KR20100090019A (en) * 2009-02-05 2010-08-13 주식회사 엘지화학 Recombinant ralstonia eutropha having an producing ability of polylactate or its copolymers and method for preparing polylactate or its copolymers using the same

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
JIA JIAN ET AL.: "Production ofpolyhydroxyalkanoates by Escherichia coli mutants with defected mixed acid fermentation pathways", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 87, no. 6, August 2010 (2010-08-01), pages 2247 - 2256 *
KEN' ICHIRO MATSUMOTO ET AL.: "Enzymatic and whole-cell synthesis of lactate-containing polyesters: toward the complete biological production of polylactate", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 85, no. 4, January 2010 (2010-01-01), pages 921 - 932 *
TAEK HO YANG ET AL.: "Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase", BIOTECHNOLOGY AND BIOENGINEERING, vol. 105, no. 1, 1 January 2010 (2010-01-01), pages 150 - 160 *
YU KYUNG JUNG ET AL.: "Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers", BIOTECHNOLOGY AND BIOENGINEERING, vol. 105, no. 1, 1 January 2010 (2010-01-01), pages 161 - 171 *
YUYANG SONG ET AL.: "Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 93, no. 5, 30 November 2011 (2011-11-30), pages 1917 - 1925 *

Also Published As

Publication number Publication date
WO2012124941A2 (en) 2012-09-20
KR20120103997A (en) 2012-09-20
KR101328567B1 (en) 2013-11-13
WO2012124941A9 (en) 2013-02-21

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