BR9910929A - Processo para produzir um vìrus mononegavirales recombinante, vìrus recombinante, e, composição - Google Patents
Processo para produzir um vìrus mononegavirales recombinante, vìrus recombinante, e, composiçãoInfo
- Publication number
- BR9910929A BR9910929A BR9910929-8A BR9910929A BR9910929A BR 9910929 A BR9910929 A BR 9910929A BR 9910929 A BR9910929 A BR 9910929A BR 9910929 A BR9910929 A BR 9910929A
- Authority
- BR
- Brazil
- Prior art keywords
- virus
- recombinant
- recombinant virus
- producing
- viruses
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/24011—Poxviridae
- C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
- C12N2710/24141—Use of virus, viral particle or viral elements as a vector
- C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/60—Vector systems having a special element relevant for transcription from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
- C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
"PROCESSO PARA PRODUZIR UM VìRUS MONONEGAVIRALES RECOMBINANTE, VìRUS RECOMBINANTE, E, COMPOSIçãO". Esta invenção refere-se a processos melhorados para produzir vírus de RNA de filamento único, sentido negativo, não segmentado, da ordem designada de vírus Mononegavirales incluindo formas de realização com relação a processos de produção destes vírus como vírus atenuados e/ou infecciosos, como vírus de sarampo (MV) e vírus sincicial respiratório (RSV). Um processo para produzir um vírus recombinante da ordem Mononegavirales compreende (a) em pelo menos uma célula hospedeira, conduzir a transfecção de uma composição de resgate que compreende (i) um vetor de transcrição compreendendo uma molécula de ácido nucleico isolada que compreende sequência de polinucleotídeo codificando um genoma ou antigenoma de um vírus de RNA de filamento único, sentido negativo, não segmentado, da ordem Mononegavirales e (ii) pelo menos um vetor de expressão que compreende pelo menos uma molécula de ácido nucleico isolada codificando as proteínas trans-atuantes necessárias para encapsidação, transcrição e replicação, em uma célula hospedeira sob condições suficientes para permitir a co-expressão destes vetores e a produção de vírus recombinante; (b) aquecer a composição de resgate transfectada para uma temperatura de choque térmico efetiva sob condições suficientes para aumentar a recuperação do vírus recombinante; e opcionalmente (c) coletar o vírus recombinante resultante.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8780098P | 1998-06-03 | 1998-06-03 | |
PCT/US1999/012292 WO1999063064A1 (en) | 1998-06-03 | 1999-06-03 | Novel methods for rescue of rna viruses |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9910929A true BR9910929A (pt) | 2001-02-20 |
Family
ID=22207342
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9910929-8A BR9910929A (pt) | 1998-06-03 | 1999-06-03 | Processo para produzir um vìrus mononegavirales recombinante, vìrus recombinante, e, composição |
Country Status (14)
Country | Link |
---|---|
EP (2) | EP1995310B1 (pt) |
JP (1) | JP4625915B2 (pt) |
KR (2) | KR100619319B1 (pt) |
CN (1) | CN1196780C (pt) |
AT (1) | ATE499438T1 (pt) |
AU (1) | AU761234B2 (pt) |
BR (1) | BR9910929A (pt) |
CA (1) | CA2333313C (pt) |
DE (1) | DE69943215D1 (pt) |
DK (1) | DK1090108T3 (pt) |
ES (1) | ES2358315T3 (pt) |
IL (3) | IL140033A0 (pt) |
MX (1) | MX228492B (pt) |
WO (1) | WO1999063064A1 (pt) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7465574B2 (en) | 1994-09-30 | 2008-12-16 | Medimmune, Llc | Recombinant RSV virus expression systems and vaccines |
US6830748B1 (en) | 1997-09-26 | 2004-12-14 | Medimmune Vaccines, Inc. | Recombinant RSV virus expression systems and vaccines |
US6495360B1 (en) * | 1999-05-28 | 2002-12-17 | Photogen, Inc. | Method for enhanced protein stabilization and for production of cell lines useful for production of such stabilized proteins |
US7361496B1 (en) | 2000-08-02 | 2008-04-22 | Wyeth | Rescue of mumps virus from cDNA |
DE60233038D1 (de) * | 2002-06-20 | 2009-09-03 | Pasteur Institut | Infektiöse cDNA eines zugelassenen Impfstammes des Masern Virus. Verwendung in immunogenen Zusammensetzungen |
WO2004113517A2 (en) * | 2003-06-09 | 2004-12-29 | Wyeth | IMPROVED METHOD FOR THE RECOVERY OF NON-SEGMENTED, NEGATIVE-STRANDED RNA VIRUSES FROM cDNA |
KR20120083480A (ko) * | 2009-10-20 | 2012-07-25 | 노파르티스 아게 | 바이러스 구제를 위한 개선된 역유전학 방법 |
EP2780034A1 (en) | 2011-11-14 | 2014-09-24 | Crucell Holland B.V. | Heterologous prime-boost immunization using measles virus-based vaccines |
CN102807989B (zh) * | 2012-08-01 | 2014-04-23 | 中国人民解放军军事医学科学院军事兽医研究所 | 犬科和/或猫科动物疫病重组活载体疫苗的制备方法 |
MX2017012389A (es) * | 2015-03-27 | 2018-09-19 | Cadila Healthcare Ltd | Vacuna del virus de la parotiditis recombinante jeryl lynn2. |
CN105039388B (zh) * | 2015-06-30 | 2019-06-18 | 浙江大学 | 重组单链负义植物病毒侵染性克隆构建方法及质粒和病毒 |
CN107058359B (zh) * | 2017-04-14 | 2019-08-13 | 北京交通大学 | 一种抗呼吸道合胞病毒药物的高通量筛选方法和应用 |
WO2018208960A1 (en) * | 2017-05-09 | 2018-11-15 | Dimension Therapeutics, Inc. | Scalable method for producing transfection reagents |
CN111849927B (zh) * | 2020-06-28 | 2022-07-08 | 浙江大学 | 高效产生重组不分节段负义rna病毒的方法及重组病毒 |
CN117625688B (zh) * | 2024-01-26 | 2024-04-30 | 中国农业科学院哈尔滨兽医研究所(中国动物卫生与流行病学中心哈尔滨分中心) | B亚型禽偏肺病毒反向遗传操作系统及其应用 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2046607C (en) * | 1989-02-14 | 2002-04-16 | Rima Kaddurah-Daouk | Inhibiting transformation of cells having elevated purine metabolic enzyme activity |
JP3602530B2 (ja) * | 1991-03-07 | 2004-12-15 | ヴァイロジェネティクス コーポレイション | 遺伝子操作したワクチン菌株 |
JPH06237773A (ja) * | 1992-12-22 | 1994-08-30 | Tonen Corp | ポックスウイルスのa型封入体(ati)プロモーター及び前期プロモーターを含んで成る外来遺伝子発現ベクター |
DK0780475T4 (da) | 1995-08-09 | 2006-10-23 | Schweiz Serum & Impfinst | Fremgangsmåde til fremstilling af infektiöse negativ-streng RNA-virus |
JPH11512609A (ja) * | 1995-09-27 | 1999-11-02 | アメリカ合衆国 | クローン化されたヌクレオチド配列からの感染性RSウイルス(respiratory syncytial virus)の生産 |
BR9712138A (pt) * | 1996-09-27 | 2000-01-18 | American Cyanamid Co | Vìrus de rna isolado, vacina, processo para imunizr um indivìduo para induzir proteção contra um vìrus de rna não segmentado, sentido negativo, de filamento único, da ordem mononegavirales e para produzir vìrus de rna, molécula de ácido nucleico isolada e composição. |
US5871803A (en) | 1997-05-30 | 1999-02-16 | Campbell Soup Company | Salt flavor enhancing compositions, food products including such compositions, and methods for preparing such products |
-
1999
- 1999-06-03 DE DE69943215T patent/DE69943215D1/de not_active Expired - Lifetime
- 1999-06-03 EP EP08006339.9A patent/EP1995310B1/en not_active Expired - Lifetime
- 1999-06-03 AT AT99927175T patent/ATE499438T1/de active
- 1999-06-03 BR BR9910929-8A patent/BR9910929A/pt not_active IP Right Cessation
- 1999-06-03 CA CA2333313A patent/CA2333313C/en not_active Expired - Fee Related
- 1999-06-03 ES ES99927175T patent/ES2358315T3/es not_active Expired - Lifetime
- 1999-06-03 CN CNB998067172A patent/CN1196780C/zh not_active Expired - Fee Related
- 1999-06-03 JP JP2000552260A patent/JP4625915B2/ja not_active Expired - Fee Related
- 1999-06-03 EP EP99927175A patent/EP1090108B1/en not_active Expired - Lifetime
- 1999-06-03 IL IL14003399A patent/IL140033A0/xx unknown
- 1999-06-03 KR KR1020067003345A patent/KR100619319B1/ko not_active IP Right Cessation
- 1999-06-03 KR KR20007013629A patent/KR100579662B1/ko not_active IP Right Cessation
- 1999-06-03 AU AU44144/99A patent/AU761234B2/en not_active Ceased
- 1999-06-03 DK DK99927175.2T patent/DK1090108T3/da active
- 1999-06-03 WO PCT/US1999/012292 patent/WO1999063064A1/en active Application Filing
-
2000
- 2000-11-21 MX MXPA00011420 patent/MX228492B/es active IP Right Grant
- 2000-12-01 IL IL140033A patent/IL140033A/en not_active IP Right Cessation
-
2007
- 2007-04-12 IL IL182512A patent/IL182512A/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
IL140033A (en) | 2010-05-17 |
EP1090108B1 (en) | 2011-02-23 |
JP2002517189A (ja) | 2002-06-18 |
AU761234B2 (en) | 2003-05-29 |
CA2333313C (en) | 2011-11-08 |
KR100619319B1 (ko) | 2006-09-06 |
CN1303426A (zh) | 2001-07-11 |
EP1995310B1 (en) | 2013-04-24 |
JP4625915B2 (ja) | 2011-02-02 |
CN1196780C (zh) | 2005-04-13 |
MX228492B (es) | 2005-06-14 |
EP1995310A1 (en) | 2008-11-26 |
EP1090108A1 (en) | 2001-04-11 |
CA2333313A1 (en) | 1999-12-09 |
AU4414499A (en) | 1999-12-20 |
KR100579662B1 (ko) | 2006-05-12 |
KR20060021943A (ko) | 2006-03-08 |
DK1090108T3 (da) | 2011-04-26 |
DE69943215D1 (de) | 2011-04-07 |
KR20010052498A (ko) | 2001-06-25 |
MXPA00011420A (es) | 2001-04-01 |
WO1999063064A1 (en) | 1999-12-09 |
IL182512A (en) | 2012-07-31 |
ES2358315T3 (es) | 2011-05-09 |
ATE499438T1 (de) | 2011-03-15 |
IL140033A0 (en) | 2002-02-10 |
IL182512A0 (en) | 2007-09-20 |
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B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 37, 13 E 8O DA LPI 9279/96. |
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B12B | Appeal against refusal [chapter 12.2 patent gazette] | ||
B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] | ||
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 15A ANUIDADE. |
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B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
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