BR9814834A - Segmentos purificado e isolado de ácido nucleico, vetor recombinante, células hospedeira recombinante, e hospedeira procariótica ou eucariótica, composições purificada, e farmacêutica, processos para detectar uma espécie de dna, para detectar uma célula bacteriana que expresse o mrna que codifica a streptococcus equisimilis hialuronan sintase, e para produzir ácido hialurÈnico, sequênica de ácido nucleico purificada e isolada, e, sequência de cdna - Google Patents

Segmentos purificado e isolado de ácido nucleico, vetor recombinante, células hospedeira recombinante, e hospedeira procariótica ou eucariótica, composições purificada, e farmacêutica, processos para detectar uma espécie de dna, para detectar uma célula bacteriana que expresse o mrna que codifica a streptococcus equisimilis hialuronan sintase, e para produzir ácido hialurÈnico, sequênica de ácido nucleico purificada e isolada, e, sequência de cdna

Info

Publication number
BR9814834A
BR9814834A BR9814834-6A BR9814834A BR9814834A BR 9814834 A BR9814834 A BR 9814834A BR 9814834 A BR9814834 A BR 9814834A BR 9814834 A BR9814834 A BR 9814834A
Authority
BR
Brazil
Prior art keywords
purified
nucleic acid
detecting
isolated nucleic
synthase
Prior art date
Application number
BR9814834-6A
Other languages
English (en)
Other versions
BR9814834B1 (pt
Inventor
Paul H Weigel
Paul Deangelis
Kshama Kumari
Original Assignee
Univ Oklahoma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Oklahoma filed Critical Univ Oklahoma
Publication of BR9814834A publication Critical patent/BR9814834A/pt
Publication of BR9814834B1 publication Critical patent/BR9814834B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/102—Pasteurellales, e.g. Actinobacillus, Pasteurella; Haemophilus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/836—Bacillus licheniformis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • Physics & Mathematics (AREA)
  • Communicable Diseases (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"SEGMENTOS PURIFICADO E ISOLADO DE áCIDO NUCLEICO, VETOR RECOMBINANTE, CéLULAS HOSPEDEIRA RECOMBINANTE, E HOSPEDEIRA PROCARIóTICA OU EUCARIóTICA, COMPOSIçõES PURIFICADA, E FARMACêUTICA, PROCESSOS PARA DETECTAR UMA ESPéCIE DE DNA, PARA DETECTAR UMA CéLULA BACTERIANA QUE EXPRESSE O MRNA QUE CODIFICA A STREPTOCOCCUS EQUISIMILIS HIALURONAN SINTASE, E PARA PRODUZIR áCIDO HIALURÈNICO, SEQUêNCIA DE áCIDO NUCLEICO PURIFICADA E ISOLADA, E, SEQUêNCIA DE cDNA" A presente invenção diz respeito a um segmento de ácido nucleido tendo um segmento de região codificadora que codifica a hialuronato sintase do Streptococcus equisimilis, enzimaticamente ativa (seHAS) e ao uso deste segmento de ácido nucleico na preparação de células recombinantes que produzem a hialuronato sintase e o seu produto de ácido hialurónico. O hialuronato também é conhecido como ácido hialurónico ou hialuronan.
BRPI9814834-6A 1997-10-31 1998-10-30 célula hospedeira recombinante. BR9814834B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US6443597P 1997-10-31 1997-10-31
US60/064435 1997-10-31
US17885198A 1998-10-26 1998-10-26
US09/178851 1998-10-26
PCT/US1998/023153 WO1999023227A2 (en) 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof

Publications (2)

Publication Number Publication Date
BR9814834A true BR9814834A (pt) 2001-07-17
BR9814834B1 BR9814834B1 (pt) 2011-08-23

Family

ID=26744517

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9814834-6A BR9814834B1 (pt) 1997-10-31 1998-10-30 célula hospedeira recombinante.

Country Status (13)

Country Link
US (10) US6833264B1 (pt)
EP (2) EP1025211B1 (pt)
JP (1) JP4731013B2 (pt)
KR (1) KR20010031682A (pt)
CN (1) CN1322121C (pt)
AT (1) ATE284954T1 (pt)
AU (2) AU762036B2 (pt)
BR (1) BR9814834B1 (pt)
CA (1) CA2307842C (pt)
DE (1) DE69828193T2 (pt)
DK (1) DK1025211T3 (pt)
ES (1) ES2235378T3 (pt)
WO (1) WO1999023227A2 (pt)

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US20030082780A1 (en) 2003-05-01
US6833264B1 (en) 2004-12-21
US20060216808A1 (en) 2006-09-28
BR9814834B1 (pt) 2011-08-23
AU1370699A (en) 1999-05-24
CN1322121C (zh) 2007-06-20
CA2307842A1 (en) 1999-05-14
US20050202540A1 (en) 2005-09-15
DE69828193D1 (de) 2005-01-20
CA2307842C (en) 2010-10-19
EP1025211B1 (en) 2004-12-15
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US7166450B2 (en) 2007-01-23
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US7087413B2 (en) 2006-08-08
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US20030073221A1 (en) 2003-04-17
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