AU2015314251A1 - Production of steviol glycosides in recombinant hosts - Google Patents
Production of steviol glycosides in recombinant hosts Download PDFInfo
- Publication number
- AU2015314251A1 AU2015314251A1 AU2015314251A AU2015314251A AU2015314251A1 AU 2015314251 A1 AU2015314251 A1 AU 2015314251A1 AU 2015314251 A AU2015314251 A AU 2015314251A AU 2015314251 A AU2015314251 A AU 2015314251A AU 2015314251 A1 AU2015314251 A1 AU 2015314251A1
- Authority
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- Australia
- Prior art keywords
- seq
- polypeptide
- set forth
- sequence set
- steviol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 239000004383 Steviol glycoside Substances 0.000 title claims abstract description 294
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- 235000019411 steviol glycoside Nutrition 0.000 title claims abstract description 288
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- 239000002243 precursor Substances 0.000 claims abstract description 51
- 238000000034 method Methods 0.000 claims abstract description 49
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- 229920001184 polypeptide Polymers 0.000 claims description 279
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- 229940032084 steviol Drugs 0.000 claims description 72
- NIKHGUQULKYIGE-UHFFFAOYSA-N kaurenoic acid Natural products C1CC2(CC3=C)CC3CCC2C2(C)C1C(C)(C(O)=O)CCC2 NIKHGUQULKYIGE-UHFFFAOYSA-N 0.000 claims description 70
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Classifications
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/30—Artificial sweetening agents
- A23L27/33—Artificial sweetening agents containing sugars or derivatives
- A23L27/36—Terpene glycosides
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
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- C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
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- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
- C12Y114/13078—Ent-kaurene oxidase (1.14.13.78)
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- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
- C12Y114/13079—Ent-kaurenoic acid oxidase (1.14.13.79)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01029—Geranylgeranyl diphosphate synthase (2.5.1.29)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
- C12Y402/03019—Ent-kaurene synthase (4.2.3.19)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y505/00—Intramolecular lyases (5.5)
- C12Y505/01—Intramolecular lyases (5.5.1)
- C12Y505/01013—Ent-copalyl diphosphate synthase (5.5.1.13)
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- C07—ORGANIC CHEMISTRY
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- Engineering & Computer Science (AREA)
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- Biomedical Technology (AREA)
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- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Priority Applications (2)
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| AU2020200887A AU2020200887B2 (en) | 2014-09-09 | 2020-02-07 | Production of steviol glycosides in recombinant hosts |
| AU2024200962A AU2024200962B2 (en) | 2014-09-09 | 2024-02-14 | Production of steviol glycosides in recombinant hosts |
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| US201462048178P | 2014-09-09 | 2014-09-09 | |
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| US201562103547P | 2015-01-14 | 2015-01-14 | |
| US62/103,547 | 2015-01-14 | ||
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| US201562148585P | 2015-04-16 | 2015-04-16 | |
| US62/148,585 | 2015-04-16 | ||
| PCT/EP2015/070620 WO2016038095A2 (en) | 2014-09-09 | 2015-09-09 | Production of steviol glycosides in recombinant hosts |
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| EP2575432B1 (en) | 2010-06-02 | 2019-08-14 | Evolva, Inc. | Recombinant production of steviol glycosides |
| CA3128532A1 (en) | 2011-08-08 | 2013-02-14 | Evolva Sa | Recombinant production of steviol glycosides |
| MY199452A (en) | 2013-02-06 | 2023-10-30 | Evolva Sa | Methods for improved production of rebaudioside d and rebaudioside m |
| BR112015019160A2 (pt) | 2013-02-11 | 2017-08-22 | Dalgaard Mikkelsen Michael | Produção de glicosídeos de esteviol em hospedeiros recombinantes |
| CA2957331A1 (en) | 2014-08-11 | 2016-02-18 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| CA2960693A1 (en) | 2014-09-09 | 2016-03-17 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| EP3250686A1 (en) | 2015-01-30 | 2017-12-06 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| WO2016146711A1 (en) | 2015-03-16 | 2016-09-22 | Dsm Ip Assets B.V. | Udp-glycosyltransferases |
| CN107666834B (zh) | 2015-04-03 | 2021-08-24 | 帝斯曼知识产权资产管理有限公司 | 甜菊醇糖苷 |
| MX2018001620A (es) | 2015-08-07 | 2018-06-11 | Evolva Sa | Produccion de glucosidos de esteviol en hospedadores recombinantes. |
| CN108138152B (zh) | 2015-10-05 | 2023-01-06 | 帝斯曼知识产权资产管理有限公司 | 贝壳杉烯酸羟化酶 |
| WO2017153538A1 (en) * | 2016-03-11 | 2017-09-14 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| US10982249B2 (en) * | 2016-04-13 | 2021-04-20 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| WO2017198681A1 (en) * | 2016-05-16 | 2017-11-23 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| EP3458599A1 (en) | 2016-05-16 | 2019-03-27 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| PT3497222T (pt) | 2016-08-12 | 2022-02-09 | Amyris Inc | Glicosiltransferase dependente de udp para produção de eficácia elevada de rebaudiosídeos |
| WO2018083338A1 (en) * | 2016-11-07 | 2018-05-11 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| KR102905512B1 (ko) | 2017-02-03 | 2025-12-29 | 테이트 앤드 라일 솔루션스 유에스에이 엘엘씨 | 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법 |
| CA3071029A1 (en) | 2017-08-11 | 2019-02-14 | Amyris, Inc. | Pisum sativum kaurene oxidase for high efficiency production of rebaudiosides |
| WO2019055325A2 (en) | 2017-09-12 | 2019-03-21 | Biocapital Holdings, Llc | BIOOGICAL DEVICES AND METHODS OF USING THE SAME TO PRODUCE STEVIOL GLYCOSIDES |
| BR112020009838A2 (pt) * | 2017-12-05 | 2020-11-17 | Evolva Sa | produção de glicosídeos de esteviol em hospedeiros recombinantes |
| SG11202007273WA (en) | 2018-01-31 | 2020-08-28 | Univ Michigan Regents | Biocatalyst and methods for synthesizing mixed disulfide conjugates of thienopyridine compounds |
| IL280302B2 (en) | 2018-07-30 | 2025-09-01 | Codexis Inc | Engineered glycosyltransferases and methods for glycosylation of steviol glycoside |
| CA3114953A1 (en) | 2018-10-15 | 2020-04-23 | Amyris, Inc. | Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides |
| EP3877519B1 (en) | 2018-11-09 | 2026-03-11 | Ginkgo Bioworks, Inc. | Biosynthesis of mogrosides |
| SG11202107656TA (en) | 2019-01-24 | 2021-08-30 | Amyris Inc | Abc transporters for the high efficiency production of rebaudiosides |
| MX2021013124A (es) * | 2019-05-03 | 2022-01-24 | Amyris Inc | Variantes del ácido kaurenoico 13-hidroxilasa (kah) y usos del mismo. |
| US12065685B1 (en) | 2019-05-13 | 2024-08-20 | Amyris, Inc. | UDP-glycosyltransferase variants and uses thereof |
| US20250290081A1 (en) * | 2020-10-22 | 2025-09-18 | Dsm Ip Assets B.V. | Microorganisms for diterpene production |
| CN112778408B (zh) * | 2021-03-02 | 2023-04-28 | 中国热带农业科学院橡胶研究所 | 橡胶树转录因子HbICE2及其编码基因与应用 |
| CN114561310B (zh) * | 2022-03-17 | 2022-12-02 | 江南大学 | 一种生产甜茶苷的酿酒酵母及其应用 |
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| JPS58149697A (ja) | 1982-02-27 | 1983-09-06 | Dainippon Ink & Chem Inc | β−１，３グリコシルステビオシドの製造方法 |
| JPS59101408A (ja) | 1982-12-02 | 1984-06-12 | Junichi Iwamura | 植物生長調整剤 |
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| US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
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| JPH03277275A (ja) | 1990-03-28 | 1991-12-09 | Dainippon Ink & Chem Inc | 新規酵素及びその酵素を用いた配糖体の製造方法 |
| US5204253A (en) | 1990-05-29 | 1993-04-20 | E. I. Du Pont De Nemours And Company | Method and apparatus for introducing biological substances into living cells |
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| AU2020200887B2 (en) | 2023-11-16 |
| US20230212630A1 (en) | 2023-07-06 |
| EP4592304A3 (en) | 2025-11-05 |
| EP3190905A2 (en) | 2017-07-19 |
| KR20170052647A (ko) | 2017-05-12 |
| SG10201901957SA (en) | 2019-04-29 |
| AU2020200887A1 (en) | 2020-02-27 |
| EP4592304A2 (en) | 2025-07-30 |
| EP3190905B1 (en) | 2025-03-12 |
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