AU2013309642B2 - Method for culturing mesenchymal stem cells - Google Patents

Method for culturing mesenchymal stem cells Download PDF

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Publication number
AU2013309642B2
AU2013309642B2 AU2013309642A AU2013309642A AU2013309642B2 AU 2013309642 B2 AU2013309642 B2 AU 2013309642B2 AU 2013309642 A AU2013309642 A AU 2013309642A AU 2013309642 A AU2013309642 A AU 2013309642A AU 2013309642 B2 AU2013309642 B2 AU 2013309642B2
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msc
stem cells
mesenchymal stem
pct
cells
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AU2013309642A1 (en
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Hong Bae Jeon
Sun Jae Kwon
Mi Yeon Lee
Won Il Oh
Yoon-Sun Yang
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Medipost Co Ltd
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Medipost Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/16—Magnesium; Mg chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Pulmonology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AU2013309642A 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells Active AU2013309642B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020120097193A KR101532556B1 (ko) 2012-09-03 2012-09-03 간엽줄기세포의 배양 방법
KR10-2012-0097193 2012-09-03
PCT/KR2013/007891 WO2014035215A1 (en) 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells

Publications (2)

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AU2013309642A1 AU2013309642A1 (en) 2015-03-26
AU2013309642B2 true AU2013309642B2 (en) 2018-10-25

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US (3) US9580687B2 (enExample)
EP (1) EP2893001B1 (enExample)
JP (1) JP6478243B2 (enExample)
KR (1) KR101532556B1 (enExample)
CN (1) CN104781394B (enExample)
AU (1) AU2013309642B2 (enExample)
CA (1) CA2884000C (enExample)
DK (1) DK2893001T3 (enExample)
ES (1) ES2755802T3 (enExample)
IN (1) IN2015DN01737A (enExample)
PL (1) PL2893001T3 (enExample)
PT (1) PT2893001T (enExample)
WO (1) WO2014035215A1 (enExample)

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HK1247639A1 (zh) * 2014-10-29 2018-09-28 R生物有限公司 培养干细胞的培养基组合物
CN105748514A (zh) * 2015-01-05 2016-07-13 洪士杰 用于治疗肝脏疾病的间叶干细胞
HK1254588A1 (zh) * 2015-02-20 2019-07-26 洪士杰 间充质干细胞用於治疗骨关节炎的用途
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US20180200301A1 (en) * 2015-05-21 2018-07-19 Shanghai Institutes For Biological Sciences Chinese Academy Of Sciences Low-Oxygen-Treated Mesenchymal Stem Cell and Use Thereof
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US11753623B2 (en) * 2017-06-05 2023-09-12 The Regents Of The University Of California Compositions for treating retinal diseases and methods for making and using them
KR102623647B1 (ko) 2017-06-27 2024-01-12 아지노모토 가부시키가이샤 리보플라빈 유도체 함유 배지
CN107384858A (zh) * 2017-08-17 2017-11-24 成都康景生物科技有限公司 一种缺氧耐受型间充质干细胞的制备方法及其应用
CN109666631A (zh) * 2017-10-17 2019-04-23 澳门大学 增强干细胞活性的培养方法、应用以及用于治疗中枢神经疾病的制剂、制备方法及治疗方法
CN109706115B (zh) * 2017-10-26 2023-05-30 中国医科大学 一种小鼠骨髓间充质干细胞细胞系的构建方法
CN108359637B (zh) * 2018-02-14 2021-09-28 浙江生创精准医疗科技有限公司 一种宫血干细胞快速扩增的方法
CN109517786B (zh) * 2018-10-18 2022-06-17 广州元帅生物科技有限公司 一种具有修复功能的多能干细胞制剂及其应用
US20220348880A1 (en) * 2019-08-30 2022-11-03 The Texas A&M University System Xenogen-Free Mesenchymal Stem Cell Compositions and Methods of Use
WO2022004938A1 (ko) * 2020-06-30 2022-01-06 주식회사 미래셀바이오 유사 중간엽 줄기세포의 제조방법
CN112147340B (zh) * 2020-09-30 2024-03-08 北京银丰鼎诚生物工程技术有限公司 一种神经干细胞免疫调节功能的检测方法
CN112375733B (zh) * 2020-11-03 2022-09-02 中国人民解放军陆军军医大学第一附属医院 可增进间充质干细胞干细胞性的植物血凝素及其在间充质干细胞扩增培养中的应用
KR102435452B1 (ko) * 2020-11-11 2022-09-07 이엔셀 주식회사 노화가 감소되고 줄기세포능이 보존된 초기 중간엽 줄기세포, 및 그 배양방법
US20240352423A1 (en) * 2021-03-10 2024-10-24 Terasaki Institute For Biomedical Innovation Methods and systems of producing products such as animal derived products
CN113502261B (zh) * 2021-07-16 2023-08-29 上海东方星际干细胞科技有限公司 一种能高效分化成脂的规模化3d低氧间充质干细胞培养体系
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Publication number Publication date
EP2893001A1 (en) 2015-07-15
CN104781394A (zh) 2015-07-15
US20150284686A1 (en) 2015-10-08
PL2893001T3 (pl) 2020-03-31
PT2893001T (pt) 2019-12-09
KR101532556B1 (ko) 2015-06-30
US10294456B2 (en) 2019-05-21
US9580687B2 (en) 2017-02-28
DK2893001T3 (da) 2019-12-02
CA2884000A1 (en) 2014-03-06
JP6478243B2 (ja) 2019-03-06
KR20140030703A (ko) 2014-03-12
WO2014035215A1 (en) 2014-03-06
US11332715B2 (en) 2022-05-17
ES2755802T3 (es) 2020-04-23
EP2893001A4 (en) 2016-03-09
IN2015DN01737A (enExample) 2015-05-29
CN104781394B (zh) 2017-12-12
US20190218517A1 (en) 2019-07-18
CA2884000C (en) 2021-08-24
US20170191036A1 (en) 2017-07-06
AU2013309642A1 (en) 2015-03-26
EP2893001B1 (en) 2019-10-30
JP2015532596A (ja) 2015-11-12

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