AU2013309642B2 - Method for culturing mesenchymal stem cells - Google Patents

Method for culturing mesenchymal stem cells Download PDF

Info

Publication number
AU2013309642B2
AU2013309642B2 AU2013309642A AU2013309642A AU2013309642B2 AU 2013309642 B2 AU2013309642 B2 AU 2013309642B2 AU 2013309642 A AU2013309642 A AU 2013309642A AU 2013309642 A AU2013309642 A AU 2013309642A AU 2013309642 B2 AU2013309642 B2 AU 2013309642B2
Authority
AU
Australia
Prior art keywords
msc
stem cells
mesenchymal stem
pct
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2013309642A
Other languages
English (en)
Other versions
AU2013309642A1 (en
Inventor
Hong Bae Jeon
Sun Jae Kwon
Mi Yeon Lee
Won Il Oh
Yoon-Sun Yang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medipost Co Ltd
Original Assignee
Medipost Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medipost Co Ltd filed Critical Medipost Co Ltd
Publication of AU2013309642A1 publication Critical patent/AU2013309642A1/en
Application granted granted Critical
Publication of AU2013309642B2 publication Critical patent/AU2013309642B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/16—Magnesium; Mg chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Transplantation (AREA)
  • Pulmonology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AU2013309642A 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells Active AU2013309642B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020120097193A KR101532556B1 (ko) 2012-09-03 2012-09-03 간엽줄기세포의 배양 방법
KR10-2012-0097193 2012-09-03
PCT/KR2013/007891 WO2014035215A1 (en) 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells

Publications (2)

Publication Number Publication Date
AU2013309642A1 AU2013309642A1 (en) 2015-03-26
AU2013309642B2 true AU2013309642B2 (en) 2018-10-25

Family

ID=50183937

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013309642A Active AU2013309642B2 (en) 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells

Country Status (13)

Country Link
US (3) US9580687B2 (enExample)
EP (1) EP2893001B1 (enExample)
JP (1) JP6478243B2 (enExample)
KR (1) KR101532556B1 (enExample)
CN (1) CN104781394B (enExample)
AU (1) AU2013309642B2 (enExample)
CA (1) CA2884000C (enExample)
DK (1) DK2893001T3 (enExample)
ES (1) ES2755802T3 (enExample)
IN (1) IN2015DN01737A (enExample)
PL (1) PL2893001T3 (enExample)
PT (1) PT2893001T (enExample)
WO (1) WO2014035215A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3140417B1 (en) * 2014-05-09 2021-04-21 Reelabs Private Limited Foetal polymix of mesenchymal stem cells under hypoxic conditions for the treatment of clinical disorders
JP6519727B2 (ja) * 2014-09-17 2019-05-29 国立大学法人山口大学 間葉系幹細胞の培養方法、および肝機能障害用治療剤の製造方法
US11000694B2 (en) 2014-10-24 2021-05-11 The Board Of Trustees Of The University Of Illinois Use of lasers for treating and reversing fibrosis
CA2968048A1 (en) * 2014-10-29 2016-05-06 R Bio Co., Ltd. Medium composition for culturing stem cells
CN105748514A (zh) * 2015-01-05 2016-07-13 洪士杰 用于治疗肝脏疾病的间叶干细胞
US10780129B2 (en) 2015-02-20 2020-09-22 National Yang-Ming University Use of mesenchymal stem cells in treating osteoarthritis
WO2016184427A1 (zh) * 2015-05-21 2016-11-24 中国科学院上海生命科学研究院 低氧处理的间充质干细胞及其应用
WO2017155325A1 (ko) * 2016-03-09 2017-09-14 울산대학교 산학협력단 Jak1 억제제를 유효성분으로 포함하는 줄기세포의 증식, 분화 촉진 또는 노화 억제용 조성물
KR102293012B1 (ko) 2016-03-31 2021-08-27 샌바이오, 인크. 줄기 세포 배양 및 치료를 위한 배지, 방법, 세포 및 분비 인자
US11753623B2 (en) * 2017-06-05 2023-09-12 The Regents Of The University Of California Compositions for treating retinal diseases and methods for making and using them
CA3068685A1 (en) 2017-06-27 2019-01-03 Ajinomoto Co., Inc. Riboflavin derivative-containing medium
CN107384858A (zh) * 2017-08-17 2017-11-24 成都康景生物科技有限公司 一种缺氧耐受型间充质干细胞的制备方法及其应用
CN109666631A (zh) * 2017-10-17 2019-04-23 澳门大学 增强干细胞活性的培养方法、应用以及用于治疗中枢神经疾病的制剂、制备方法及治疗方法
CN109706115B (zh) * 2017-10-26 2023-05-30 中国医科大学 一种小鼠骨髓间充质干细胞细胞系的构建方法
CN108359637B (zh) * 2018-02-14 2021-09-28 浙江生创精准医疗科技有限公司 一种宫血干细胞快速扩增的方法
CN109517786B (zh) * 2018-10-18 2022-06-17 广州元帅生物科技有限公司 一种具有修复功能的多能干细胞制剂及其应用
US20220348880A1 (en) * 2019-08-30 2022-11-03 The Texas A&M University System Xenogen-Free Mesenchymal Stem Cell Compositions and Methods of Use
WO2022004938A1 (ko) * 2020-06-30 2022-01-06 주식회사 미래셀바이오 유사 중간엽 줄기세포의 제조방법
CN112147340B (zh) * 2020-09-30 2024-03-08 北京银丰鼎诚生物工程技术有限公司 一种神经干细胞免疫调节功能的检测方法
CN112375733B (zh) * 2020-11-03 2022-09-02 中国人民解放军陆军军医大学第一附属医院 可增进间充质干细胞干细胞性的植物血凝素及其在间充质干细胞扩增培养中的应用
KR102435452B1 (ko) * 2020-11-11 2022-09-07 이엔셀 주식회사 노화가 감소되고 줄기세포능이 보존된 초기 중간엽 줄기세포, 및 그 배양방법
US20240352423A1 (en) * 2021-03-10 2024-10-24 Terasaki Institute For Biomedical Innovation Methods and systems of producing products such as animal derived products
CN113502261B (zh) * 2021-07-16 2023-08-29 上海东方星际干细胞科技有限公司 一种能高效分化成脂的规模化3d低氧间充质干细胞培养体系
CN116218770B (zh) * 2022-12-30 2023-11-24 苏州科为康生物医药科技有限公司 一种间充质干细胞的制备方法和应用
CN117660325B (zh) * 2024-01-31 2024-04-19 苏州科为康生物医药科技有限公司 一种制备脐带血msc的培养基及其方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009014272A1 (en) * 2007-07-20 2009-01-29 Dongguk University Industry-Academic Cooperation Foundation Method for the preparation of dermal papilla tissue employing mesenchymal stem cells
EP2039758A1 (en) * 2007-09-21 2009-03-25 Universität Leipzig Cell culture medium and method for culturing stem cells and progenitor cells
WO2010150094A1 (en) * 2009-06-26 2010-12-29 Life & Light Limited Mesenchymal stem cells grown under hypoxic conditions: compositions, methods and uses therefor

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE312615T1 (de) 2001-08-14 2005-12-15 Medipost Co Ltd Zusammensetzung zur behandlung von schäden des gelenkknorpels
CA2463850C (en) * 2001-10-18 2013-03-26 Lifecell Corporation Remodeling of tissues and organs
ES2287447T3 (es) 2002-02-19 2007-12-16 Medipost, Co., Ltd. Metodos de aislamiento y expansion del cultivo de celulas troncales/madre mesenquimatosas a partir de sangre del cordon umbilical, y metodo de diferenciacion de celulas troncales/madre mesenquimatosas derivadas de sangre del cordon umbilical en diversos tejidos mesenquimatosos.
US20050281788A1 (en) * 2002-07-30 2005-12-22 Cosimo De Bari Compositions comprising muscle progenitor cells and uses thereof
EP1636388A2 (en) * 2003-06-06 2006-03-22 Wyeth Methods and materials for identifying agents which modulate bone remodeling and agents identified thereby
JP2006055106A (ja) * 2004-08-23 2006-03-02 National Institute Of Advanced Industrial & Technology ヒト血清培地を用いるヒト骨髄由来間葉系幹細胞培養法
ES2708933T3 (es) * 2005-12-29 2019-04-12 Celularity Inc Poblaciones de células madre placentarias
US20070178073A1 (en) * 2006-02-01 2007-08-02 Samsung Life Public Welfare Foundation Composition Comprising Separated or Proliferated Cells from Umbilical Cord Blood for Treating Developmental and/or Chronic Lung Disease
KR101569168B1 (ko) * 2007-02-12 2015-11-13 안트로제네시스 코포레이션 태반 줄기세포를 이용한 염증 질환의 치료
US8114668B2 (en) 2007-05-14 2012-02-14 Cardiac Pacemakers, Inc. Composition for cold storage of stem cells
GB0715648D0 (en) * 2007-08-10 2007-09-19 Cambridge Silicon Radio Ltd Channel decoding using similarity matching
EP2489727A4 (en) 2009-10-08 2013-05-08 Seoul Nat Univ Hospital METHOD FOR CREATING A HIGH ACTIVITY OF HUMAN ADIPOSITAS STEM CELLS AND MEDIUM THEREFOR
DK2602310T3 (da) * 2010-08-03 2020-02-24 Univ Sapporo Medical Autoserum-indeholdende knoglemarvcelledyrkningssystem, autoserum-indeholdende knoglemarvcelledyrkningsfremgangsmåde, og fremgangsmåde til fremstilling af en lægemiddelsammensætning omfattende (...)
US20120052577A1 (en) * 2010-08-31 2012-03-01 The Regents Of The University Of California Culture system for stem cell propagation and neural and oligodendrocyte specification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009014272A1 (en) * 2007-07-20 2009-01-29 Dongguk University Industry-Academic Cooperation Foundation Method for the preparation of dermal papilla tissue employing mesenchymal stem cells
EP2039758A1 (en) * 2007-09-21 2009-03-25 Universität Leipzig Cell culture medium and method for culturing stem cells and progenitor cells
WO2010150094A1 (en) * 2009-06-26 2010-12-29 Life & Light Limited Mesenchymal stem cells grown under hypoxic conditions: compositions, methods and uses therefor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BASCIANO, L. ET AL., "Long term culture of mesenchymal stem cells in hypoxi a promotes a genetic program maintaining their undifferentiated and multipot ent status.", BMC CELL BIOLOGY, (2011-03-30), vol. 12, no. 12, pages 1 - 12 *
ZHIHUI XU ET AL, "Effect of the calcium sensing receptor on rat bone marrow-derived mesenchymal stem cell proliferation through the ERK1/2 pathway", MOLECULAR BIOLOGY REPORTS, (2012-02-08), vol. 39, no. 7, pages 7271 - 7279 *

Also Published As

Publication number Publication date
US9580687B2 (en) 2017-02-28
US20170191036A1 (en) 2017-07-06
CA2884000C (en) 2021-08-24
WO2014035215A1 (en) 2014-03-06
CA2884000A1 (en) 2014-03-06
JP2015532596A (ja) 2015-11-12
AU2013309642A1 (en) 2015-03-26
CN104781394A (zh) 2015-07-15
US20190218517A1 (en) 2019-07-18
IN2015DN01737A (enExample) 2015-05-29
CN104781394B (zh) 2017-12-12
EP2893001A1 (en) 2015-07-15
US20150284686A1 (en) 2015-10-08
ES2755802T3 (es) 2020-04-23
EP2893001B1 (en) 2019-10-30
PL2893001T3 (pl) 2020-03-31
JP6478243B2 (ja) 2019-03-06
EP2893001A4 (en) 2016-03-09
PT2893001T (pt) 2019-12-09
DK2893001T3 (da) 2019-12-02
KR101532556B1 (ko) 2015-06-30
US10294456B2 (en) 2019-05-21
US11332715B2 (en) 2022-05-17
KR20140030703A (ko) 2014-03-12

Similar Documents

Publication Publication Date Title
US11332715B2 (en) Method for culturing mesenchymal stem cells
WO2014046417A1 (en) Method for preparing mesenchymal stem cell aggregates
JP6947430B2 (ja) 細胞培養方法
US20100330047A1 (en) Mesenchymal Stem Cells Grown Under Hypoxic Conditions: Compositions, Methods and Uses Therefor
AU2014315919B8 (en) Method for culturing mesenchymal stem cells according to cell size
WO2006001428A1 (ja) 羊膜由来細胞の分化誘導方法及びその利用
CN112469815A (zh) 牙髓来源细胞的制造方法
JP5431146B2 (ja) 免疫学的寛容および調節性前駆細胞
JP2019509065A (ja) コロニー形成培地及びその使用
JP5388297B2 (ja) アディポクラスター
CN113249316A (zh) 脐带/胎盘间充质干细胞源性外泌体的制备方法及应用
US8951795B2 (en) Revascularization cells derived from mononuclear cells, and method of inducing differentiation thereof
WO2020190672A1 (en) Cardiomyocyte-derived exosomes inducing regeneration of damaged heart tissue
Zhao et al. Transdifferentiation of autologous bone marrow cells on a collagen-poly (ε-caprolactone) scaffold for tissue engineering in complete lack of native urothelium
WO2013123607A1 (zh) 用于成体干细胞的体外无血清培养的方法和培养基
CN116437940A (zh) 骨形成组合物和其用途
CN102533641B (zh) 体外无血清成体干细胞放大培养的方法及其培养液
Nishio et al. Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge
WO2024128222A1 (ja) 組成物およびその利用
HK40038967A (en) Method for producing dental pulp-derived cells

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)