KR101532556B1 - 간엽줄기세포의 배양 방법 - Google Patents

간엽줄기세포의 배양 방법 Download PDF

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KR101532556B1
KR101532556B1 KR1020120097193A KR20120097193A KR101532556B1 KR 101532556 B1 KR101532556 B1 KR 101532556B1 KR 1020120097193 A KR1020120097193 A KR 1020120097193A KR 20120097193 A KR20120097193 A KR 20120097193A KR 101532556 B1 KR101532556 B1 KR 101532556B1
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mesenchymal stem
medium
stem cells
cells
cell
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KR20140030703A (ko
Inventor
양윤선
오원일
권순재
이미연
전홍배
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메디포스트(주)
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Priority to KR1020120097193A priority Critical patent/KR101532556B1/ko
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Priority to EP13833872.8A priority patent/EP2893001B1/en
Priority to ES13833872T priority patent/ES2755802T3/es
Priority to JP2015529690A priority patent/JP6478243B2/ja
Priority to IN1737DEN2015 priority patent/IN2015DN01737A/en
Priority to PCT/KR2013/007891 priority patent/WO2014035215A1/en
Priority to CN201380045750.4A priority patent/CN104781394B/zh
Priority to CA2884000A priority patent/CA2884000C/en
Priority to US14/425,220 priority patent/US9580687B2/en
Priority to AU2013309642A priority patent/AU2013309642B2/en
Priority to DK13833872T priority patent/DK2893001T3/da
Priority to PT138338728T priority patent/PT2893001T/pt
Priority to PL13833872T priority patent/PL2893001T3/pl
Priority to US14/425,200 priority patent/US20190199132A1/en
Publication of KR20140030703A publication Critical patent/KR20140030703A/ko
Application granted granted Critical
Publication of KR101532556B1 publication Critical patent/KR101532556B1/ko
Priority to US15/403,457 priority patent/US10294456B2/en
Priority to US16/370,102 priority patent/US11332715B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
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    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
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    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/16—Magnesium; Mg chelators
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals

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  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • General Engineering & Computer Science (AREA)
  • Physical Education & Sports Medicine (AREA)
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  • Epidemiology (AREA)
  • Pulmonology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020120097193A 2012-09-03 2012-09-03 간엽줄기세포의 배양 방법 Active KR101532556B1 (ko)

Priority Applications (16)

Application Number Priority Date Filing Date Title
KR1020120097193A KR101532556B1 (ko) 2012-09-03 2012-09-03 간엽줄기세포의 배양 방법
DK13833872T DK2893001T3 (da) 2012-09-03 2013-09-02 Fremgangsmåde til dyrkning af mesenkymale stamceller
JP2015529690A JP6478243B2 (ja) 2012-09-03 2013-09-02 間葉系幹細胞を培養するための方法
IN1737DEN2015 IN2015DN01737A (enExample) 2012-09-03 2013-09-02
PCT/KR2013/007891 WO2014035215A1 (en) 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells
CN201380045750.4A CN104781394B (zh) 2012-09-03 2013-09-02 用于培养间充质干细胞的方法
CA2884000A CA2884000C (en) 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells
US14/425,220 US9580687B2 (en) 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells
EP13833872.8A EP2893001B1 (en) 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells
ES13833872T ES2755802T3 (es) 2012-09-03 2013-09-02 Método para el cultivo de células madre mesenquimatosas
PT138338728T PT2893001T (pt) 2012-09-03 2013-09-02 Método para cultura de células estaminais mesenquimatosas
PL13833872T PL2893001T3 (pl) 2012-09-03 2013-09-02 Sposób hodowli mezenchymalnych komórek macierzystych
AU2013309642A AU2013309642B2 (en) 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells
US14/425,200 US20190199132A1 (en) 2012-09-03 2014-02-24 Contactless power transmission apparatus and contactless power transmission method
US15/403,457 US10294456B2 (en) 2012-09-03 2017-01-11 Method for culturing mesenchymal stem cells
US16/370,102 US11332715B2 (en) 2012-09-03 2019-03-29 Method for culturing mesenchymal stem cells

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Application Number Priority Date Filing Date Title
KR1020120097193A KR101532556B1 (ko) 2012-09-03 2012-09-03 간엽줄기세포의 배양 방법

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KR20140030703A KR20140030703A (ko) 2014-03-12
KR101532556B1 true KR101532556B1 (ko) 2015-06-30

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US (3) US9580687B2 (enExample)
EP (1) EP2893001B1 (enExample)
JP (1) JP6478243B2 (enExample)
KR (1) KR101532556B1 (enExample)
CN (1) CN104781394B (enExample)
AU (1) AU2013309642B2 (enExample)
CA (1) CA2884000C (enExample)
DK (1) DK2893001T3 (enExample)
ES (1) ES2755802T3 (enExample)
IN (1) IN2015DN01737A (enExample)
PL (1) PL2893001T3 (enExample)
PT (1) PT2893001T (enExample)
WO (1) WO2014035215A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3140417B1 (en) * 2014-05-09 2021-04-21 Reelabs Private Limited Foetal polymix of mesenchymal stem cells under hypoxic conditions for the treatment of clinical disorders
JP6519727B2 (ja) * 2014-09-17 2019-05-29 国立大学法人山口大学 間葉系幹細胞の培養方法、および肝機能障害用治療剤の製造方法
US11000694B2 (en) 2014-10-24 2021-05-11 The Board Of Trustees Of The University Of Illinois Use of lasers for treating and reversing fibrosis
JP6494756B2 (ja) * 2014-10-29 2019-04-03 アール バイオ カンパニー リミテッドＲ Ｂｉｏ Ｃｏ．， Ｌｔｄ． 幹細胞培養のための培地組成物
TWI740812B (zh) * 2015-01-05 2021-10-01 國立陽明大學 用於治療肝臟疾病的間葉幹細胞
WO2016131430A1 (en) * 2015-02-20 2016-08-25 Shih-Chieh Hung Use of mesenchymal stem cells in treating osteoarthritis
WO2016184427A1 (zh) * 2015-05-21 2016-11-24 中国科学院上海生命科学研究院 低氧处理的间充质干细胞及其应用
EP3461887A4 (en) * 2016-03-09 2020-01-22 University Of Ulsan Foundation For Industry Cooperation COMPOSITION FOR PROLIFERATION, PROMOTION OF DIFFERENTIATION OR SENESCENCE INHIBITION OF STEM CELLS WITH JAK1 INHIBITOR AS AN ACTIVE SUBSTANCE
KR102555611B1 (ko) * 2016-03-31 2023-07-13 샌바이오, 인크. 줄기 세포 배양 및 치료를 위한 배지, 방법, 세포 및 분비 인자
JP7350314B2 (ja) * 2017-06-05 2023-09-26 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 網膜疾患を処置するための組成物ならびにそれを作製および使用するための方法
CA3068685A1 (en) 2017-06-27 2019-01-03 Ajinomoto Co., Inc. Riboflavin derivative-containing medium
CN107384858A (zh) * 2017-08-17 2017-11-24 成都康景生物科技有限公司 一种缺氧耐受型间充质干细胞的制备方法及其应用
CN109666631A (zh) * 2017-10-17 2019-04-23 澳门大学 增强干细胞活性的培养方法、应用以及用于治疗中枢神经疾病的制剂、制备方法及治疗方法
CN109706115B (zh) * 2017-10-26 2023-05-30 中国医科大学 一种小鼠骨髓间充质干细胞细胞系的构建方法
CN108359637B (zh) * 2018-02-14 2021-09-28 浙江生创精准医疗科技有限公司 一种宫血干细胞快速扩增的方法
CN109517786B (zh) * 2018-10-18 2022-06-17 广州元帅生物科技有限公司 一种具有修复功能的多能干细胞制剂及其应用
CA3149572A1 (en) * 2019-08-30 2021-03-04 Ashlee WATTS Xenogen-free mesenchymal stem cell compositions and methods of use
WO2022004938A1 (ko) * 2020-06-30 2022-01-06 주식회사 미래셀바이오 유사 중간엽 줄기세포의 제조방법
CN112147340B (zh) * 2020-09-30 2024-03-08 北京银丰鼎诚生物工程技术有限公司 一种神经干细胞免疫调节功能的检测方法
CN112375733B (zh) * 2020-11-03 2022-09-02 中国人民解放军陆军军医大学第一附属医院 可增进间充质干细胞干细胞性的植物血凝素及其在间充质干细胞扩增培养中的应用
KR102435452B1 (ko) * 2020-11-11 2022-09-07 이엔셀 주식회사 노화가 감소되고 줄기세포능이 보존된 초기 중간엽 줄기세포, 및 그 배양방법
WO2022192441A1 (en) * 2021-03-10 2022-09-15 Terasaki Institute For Biomedical Innovation Systems and methods of producing fat tissue for cell-based meat products
CN113502261B (zh) * 2021-07-16 2023-08-29 上海东方星际干细胞科技有限公司 一种能高效分化成脂的规模化3d低氧间充质干细胞培养体系
CN116218770B (zh) * 2022-12-30 2023-11-24 苏州科为康生物医药科技有限公司 一种间充质干细胞的制备方法和应用
CN117660325B (zh) * 2024-01-31 2024-04-19 苏州科为康生物医药科技有限公司 一种制备脐带血msc的培养基及其方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110038593A (ko) * 2009-10-08 2011-04-14 서울대학교병원 인간 지방 줄기세포의 고활성을 유도하는 방법 및 배지

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1416944B1 (en) 2001-08-14 2005-12-14 Medipost, Co., Ltd. Composition for treatment of articular cartilage damage
ES2672815T3 (es) * 2001-10-18 2018-06-18 Lifecell Corporation Remodelación de tejidos y órganos
WO2003070922A1 (en) 2002-02-19 2003-08-28 Medipost Co., Ltd. Isolation and culture-expansion methods of mesenchymal stem/progenitor cells from umbilical cord blood, and differentiation method of umbilical cord blood-derived mesenchymal stem/progenitor cells into various mesenchymal tissues
US20050281788A1 (en) * 2002-07-30 2005-12-22 Cosimo De Bari Compositions comprising muscle progenitor cells and uses thereof
JP2007525953A (ja) * 2003-06-06 2007-09-13 ワイス 骨リモデリングを調節する物質を同定するための方法および材料、ならびにそれによって同定された物質
JP2006055106A (ja) 2004-08-23 2006-03-02 National Institute Of Advanced Industrial & Technology ヒト血清培地を用いるヒト骨髄由来間葉系幹細胞培養法
ES2671355T3 (es) * 2005-12-29 2018-06-06 Anthrogenesis Corporation Poblaciones de células madre placentarias
US20070178073A1 (en) * 2006-02-01 2007-08-02 Samsung Life Public Welfare Foundation Composition Comprising Separated or Proliferated Cells from Umbilical Cord Blood for Treating Developmental and/or Chronic Lung Disease
SI2120977T1 (sl) * 2007-02-12 2014-01-31 Anthrogenesis Coroporation Zdravljenje vnetnih bolezni z uporabo placentarnih matičnih celic
US8114668B2 (en) 2007-05-14 2012-02-14 Cardiac Pacemakers, Inc. Composition for cold storage of stem cells
KR20100110905A (ko) * 2007-07-20 2010-10-13 동국대학교 산학협력단 중간엽 줄기세포를 이용하여 모유두 조직을 제조하는 방법
GB0715648D0 (en) 2007-08-10 2007-09-19 Cambridge Silicon Radio Ltd Channel decoding using similarity matching
ATE479741T1 (de) 2007-09-21 2010-09-15 Univ Leipzig Zellkulturenmedium und verfahren zur kultivierung von stammzellen und vorläuferzellen
US20100330047A1 (en) * 2009-06-26 2010-12-30 Life & Light Ltd. Mesenchymal Stem Cells Grown Under Hypoxic Conditions: Compositions, Methods and Uses Therefor
PL2602310T3 (pl) * 2010-08-03 2020-07-13 Sapporo Medical University System hodowli komórek szpiku kostnego zawierających surowicę autologiczną, sposób hodowli komórek szpiku kostnego zawierających surowicę autologiczną i sposób wytwarzania kompozycji leczniczej zawierającej jako składnik aktywny hodowane komórki szpiku kostnego zawierające surowicę autologiczną
US20120052577A1 (en) * 2010-08-31 2012-03-01 The Regents Of The University Of California Culture system for stem cell propagation and neural and oligodendrocyte specification

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110038593A (ko) * 2009-10-08 2011-04-14 서울대학교병원 인간 지방 줄기세포의 고활성을 유도하는 방법 및 배지

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DK2893001T3 (da) 2019-12-02
JP6478243B2 (ja) 2019-03-06
PT2893001T (pt) 2019-12-09
IN2015DN01737A (enExample) 2015-05-29
EP2893001B1 (en) 2019-10-30
AU2013309642B2 (en) 2018-10-25
WO2014035215A1 (en) 2014-03-06
US10294456B2 (en) 2019-05-21
JP2015532596A (ja) 2015-11-12
AU2013309642A1 (en) 2015-03-26
CN104781394A (zh) 2015-07-15
US20150284686A1 (en) 2015-10-08
US11332715B2 (en) 2022-05-17
CA2884000C (en) 2021-08-24
EP2893001A1 (en) 2015-07-15
US20170191036A1 (en) 2017-07-06
KR20140030703A (ko) 2014-03-12
ES2755802T3 (es) 2020-04-23
US20190218517A1 (en) 2019-07-18
CN104781394B (zh) 2017-12-12
EP2893001A4 (en) 2016-03-09
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US9580687B2 (en) 2017-02-28

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