IN2015DN01737A - - Google Patents

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Publication number
IN2015DN01737A
IN2015DN01737A IN1737DEN2015A IN2015DN01737A IN 2015DN01737 A IN2015DN01737 A IN 2015DN01737A IN 1737DEN2015 A IN1737DEN2015 A IN 1737DEN2015A IN 2015DN01737 A IN2015DN01737 A IN 2015DN01737A
Authority
IN
India
Prior art keywords
stem cells
mesenchymal stem
culturing
concentration
viability
Prior art date
Application number
Inventor
Yoon Sun Yang
Won Il Oh
Sun Jae Kwon
Mi Yeon Lee
Hong Bae Jeon
Original Assignee
Medipost Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medipost Co Ltd filed Critical Medipost Co Ltd
Publication of IN2015DN01737A publication Critical patent/IN2015DN01737A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/16—Magnesium; Mg chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Pulmonology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Disclosed is a method for culturing mesenchymal stem cells comprising culturing mesenchymal stem cells in a medium containing calcium in a concentration of from 2.1 to 3.8 mM and magnesium in a concentration of from 1.0 to 3.0 mM under a hypoxic condition of 2 to 5 % oxygen. The culturing method can increase the population of mesenchymal stem cells even with a small number of passages by improving mesenchymal stem cells in proliferative capacity and viability. In addition the mesenchymal stem cells prepared by the culturing method are effectively used not only as a safe cell therapeutic agent due to their lacking immunogenicity but also as a cartilage regenerating medicine owing to their excellent secretion of cytokines.
IN1737DEN2015 2012-09-03 2013-09-02 IN2015DN01737A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020120097193A KR101532556B1 (en) 2012-09-03 2012-09-03 Method for culturing mesenchymal stem cells
PCT/KR2013/007891 WO2014035215A1 (en) 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells

Publications (1)

Publication Number Publication Date
IN2015DN01737A true IN2015DN01737A (en) 2015-05-29

Family

ID=50183937

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1737DEN2015 IN2015DN01737A (en) 2012-09-03 2013-09-02

Country Status (13)

Country Link
US (3) US9580687B2 (en)
EP (1) EP2893001B1 (en)
JP (1) JP6478243B2 (en)
KR (1) KR101532556B1 (en)
CN (1) CN104781394B (en)
AU (1) AU2013309642B2 (en)
CA (1) CA2884000C (en)
DK (1) DK2893001T3 (en)
ES (1) ES2755802T3 (en)
IN (1) IN2015DN01737A (en)
PL (1) PL2893001T3 (en)
PT (1) PT2893001T (en)
WO (1) WO2014035215A1 (en)

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WO2015170347A2 (en) * 2014-05-09 2015-11-12 Reelabs Private Limited Foetal polymix of mesenchymal stem cells under hypoxic conditions for the treatment of clinical disorders
JP6519727B2 (en) * 2014-09-17 2019-05-29 国立大学法人山口大学 Method of culturing mesenchymal stem cells, and method of producing therapeutic agent for liver dysfunction
US11000694B2 (en) 2014-10-24 2021-05-11 The Board Of Trustees Of The University Of Illinois Use of lasers for treating and reversing fibrosis
CN107406829A (en) * 2014-10-29 2017-11-28 R生物有限公司 Cultivate the culture media composition of stem cell
CN105748514A (en) * 2015-01-05 2016-07-13 洪士杰 Mesenchymal stem cell used for curing hepatic diseases
TWI723977B (en) * 2015-02-20 2021-04-11 國立陽明大學 Use of mesenchymal stem cells in treating osteoarthritis
CN106167789B (en) * 2015-05-21 2020-08-04 中国科学院上海营养与健康研究所 Hypoxia-treated mesenchymal stem cells and application thereof
KR102409483B1 (en) * 2016-03-31 2022-06-16 샌바이오, 인크. Medium, methods, cells and secreted factors for stem cell culture and therapy
TW201920654A (en) * 2017-06-05 2019-06-01 加州大學董事會 Compositions for treating retinal diseases and methods for making and using them
CN107384858A (en) * 2017-08-17 2017-11-24 成都康景生物科技有限公司 A kind of preparation method and applications of hypoxic tolerance type mescenchymal stem cell
CN109666631A (en) * 2017-10-17 2019-04-23 澳门大学 Enhance the cultural method of Stem Cell Activity, using and for treating preparation, preparation method and the treatment method of central nervous diseases
CN109706115B (en) * 2017-10-26 2023-05-30 中国医科大学 Construction method of mouse bone marrow mesenchymal stem cell line
CN108359637B (en) * 2018-02-14 2021-09-28 浙江生创精准医疗科技有限公司 Method for rapidly amplifying uterine blood stem cells
CN109517786B (en) * 2018-10-18 2022-06-17 广州元帅生物科技有限公司 Pluripotent stem cell preparation with repairing function and application thereof
US20220348880A1 (en) * 2019-08-30 2022-11-03 The Texas A&M University System Xenogen-Free Mesenchymal Stem Cell Compositions and Methods of Use
WO2022004938A1 (en) * 2020-06-30 2022-01-06 주식회사 미래셀바이오 Method for preparing mesenchymal-like stem cells
CN112147340B (en) * 2020-09-30 2024-03-08 北京银丰鼎诚生物工程技术有限公司 Detection method for neural stem cell immunoregulation function
CN112375733B (en) * 2020-11-03 2022-09-02 中国人民解放军陆军军医大学第一附属医院 Phytohemagglutinin capable of improving stem cell performance of mesenchymal stem cells and application of phytohemagglutinin in amplification culture of mesenchymal stem cells
CN113502261B (en) * 2021-07-16 2023-08-29 上海东方星际干细胞科技有限公司 Large-scale 3D low-oxygen mesenchymal stem cell culture system capable of efficiently differentiating into lipid
CN116218770B (en) * 2022-12-30 2023-11-24 苏州科为康生物医药科技有限公司 Preparation method and application of mesenchymal stem cells
CN117660325B (en) * 2024-01-31 2024-04-19 苏州科为康生物医药科技有限公司 Culture medium for preparing umbilical cord blood MSC and method thereof

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CN1272022C (en) 2001-08-14 2006-08-30 美迪宝斯特有限公司 Composition for treatment of articular cartilage damage
JP4451658B2 (en) * 2001-10-18 2010-04-14 ライフセル コーポレーション Tissue and organ remodeling
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US20120052577A1 (en) * 2010-08-31 2012-03-01 The Regents Of The University Of California Culture system for stem cell propagation and neural and oligodendrocyte specification

Also Published As

Publication number Publication date
PT2893001T (en) 2019-12-09
EP2893001A1 (en) 2015-07-15
ES2755802T3 (en) 2020-04-23
DK2893001T3 (en) 2019-12-02
US20190218517A1 (en) 2019-07-18
CA2884000C (en) 2021-08-24
CA2884000A1 (en) 2014-03-06
CN104781394B (en) 2017-12-12
US20150284686A1 (en) 2015-10-08
WO2014035215A1 (en) 2014-03-06
AU2013309642B2 (en) 2018-10-25
JP6478243B2 (en) 2019-03-06
PL2893001T3 (en) 2020-03-31
EP2893001A4 (en) 2016-03-09
CN104781394A (en) 2015-07-15
KR101532556B1 (en) 2015-06-30
US10294456B2 (en) 2019-05-21
EP2893001B1 (en) 2019-10-30
JP2015532596A (en) 2015-11-12
KR20140030703A (en) 2014-03-12
AU2013309642A1 (en) 2015-03-26
US9580687B2 (en) 2017-02-28
US11332715B2 (en) 2022-05-17
US20170191036A1 (en) 2017-07-06

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