PT2893001T - Método para cultura de células estaminais mesenquimatosas - Google Patents

Método para cultura de células estaminais mesenquimatosas

Info

Publication number
PT2893001T
PT2893001T PT138338728T PT13833872T PT2893001T PT 2893001 T PT2893001 T PT 2893001T PT 138338728 T PT138338728 T PT 138338728T PT 13833872 T PT13833872 T PT 13833872T PT 2893001 T PT2893001 T PT 2893001T
Authority
PT
Portugal
Prior art keywords
stem cells
mesenchymal stem
culturing mesenchymal
culturing
cells
Prior art date
Application number
PT138338728T
Other languages
English (en)
Inventor
Yang Yoon-Sun
Il Oh Won
Jae Kwon Sun
Yeon Lee Mi
Bae Jeon Hong
Original Assignee
Medipost Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medipost Co Ltd filed Critical Medipost Co Ltd
Publication of PT2893001T publication Critical patent/PT2893001T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/16—Magnesium; Mg chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Pulmonology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
PT138338728T 2012-09-03 2013-09-02 Método para cultura de células estaminais mesenquimatosas PT2893001T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020120097193A KR101532556B1 (ko) 2012-09-03 2012-09-03 간엽줄기세포의 배양 방법

Publications (1)

Publication Number Publication Date
PT2893001T true PT2893001T (pt) 2019-12-09

Family

ID=50183937

Family Applications (1)

Application Number Title Priority Date Filing Date
PT138338728T PT2893001T (pt) 2012-09-03 2013-09-02 Método para cultura de células estaminais mesenquimatosas

Country Status (13)

Country Link
US (3) US9580687B2 (pt)
EP (1) EP2893001B1 (pt)
JP (1) JP6478243B2 (pt)
KR (1) KR101532556B1 (pt)
CN (1) CN104781394B (pt)
AU (1) AU2013309642B2 (pt)
CA (1) CA2884000C (pt)
DK (1) DK2893001T3 (pt)
ES (1) ES2755802T3 (pt)
IN (1) IN2015DN01737A (pt)
PL (1) PL2893001T3 (pt)
PT (1) PT2893001T (pt)
WO (1) WO2014035215A1 (pt)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3140417B1 (en) * 2014-05-09 2021-04-21 Reelabs Private Limited Foetal polymix of mesenchymal stem cells under hypoxic conditions for the treatment of clinical disorders
JP6519727B2 (ja) * 2014-09-17 2019-05-29 国立大学法人山口大学 間葉系幹細胞の培養方法、および肝機能障害用治療剤の製造方法
US11000694B2 (en) 2014-10-24 2021-05-11 The Board Of Trustees Of The University Of Illinois Use of lasers for treating and reversing fibrosis
WO2016068596A1 (ko) * 2014-10-29 2016-05-06 주식회사 알바이오 줄기세포 배양을 위한 배지조성물
TWI740812B (zh) * 2015-01-05 2021-10-01 國立陽明大學 用於治療肝臟疾病的間葉幹細胞
JP2018512061A (ja) * 2015-02-20 2018-05-10 ナショナル ヤン−ミン ユニバーシティ 骨関節炎処置のための間葉幹細胞の使用
CN106167789B (zh) * 2015-05-21 2020-08-04 中国科学院上海营养与健康研究所 低氧处理的间充质干细胞及其应用
KR102705670B1 (ko) 2016-03-31 2024-09-11 샌바이오, 인크. 줄기 세포 배양 및 치료를 위한 배지, 방법, 세포 및 분비 인자
JP7350314B2 (ja) * 2017-06-05 2023-09-26 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 網膜疾患を処置するための組成物ならびにそれを作製および使用するための方法
CN107384858A (zh) * 2017-08-17 2017-11-24 成都康景生物科技有限公司 一种缺氧耐受型间充质干细胞的制备方法及其应用
CN109666631A (zh) * 2017-10-17 2019-04-23 澳门大学 增强干细胞活性的培养方法、应用以及用于治疗中枢神经疾病的制剂、制备方法及治疗方法
CN109706115B (zh) * 2017-10-26 2023-05-30 中国医科大学 一种小鼠骨髓间充质干细胞细胞系的构建方法
CN108359637B (zh) * 2018-02-14 2021-09-28 浙江生创精准医疗科技有限公司 一种宫血干细胞快速扩增的方法
CN109517786B (zh) * 2018-10-18 2022-06-17 广州元帅生物科技有限公司 一种具有修复功能的多能干细胞制剂及其应用
CA3149572A1 (en) * 2019-08-30 2021-03-04 Ashlee WATTS Xenogen-free mesenchymal stem cell compositions and methods of use
WO2022004938A1 (ko) * 2020-06-30 2022-01-06 주식회사 미래셀바이오 유사 중간엽 줄기세포의 제조방법
CN112147340B (zh) * 2020-09-30 2024-03-08 北京银丰鼎诚生物工程技术有限公司 一种神经干细胞免疫调节功能的检测方法
CN112375733B (zh) * 2020-11-03 2022-09-02 中国人民解放军陆军军医大学第一附属医院 可增进间充质干细胞干细胞性的植物血凝素及其在间充质干细胞扩增培养中的应用
CN113502261B (zh) * 2021-07-16 2023-08-29 上海东方星际干细胞科技有限公司 一种能高效分化成脂的规模化3d低氧间充质干细胞培养体系
CN116218770B (zh) * 2022-12-30 2023-11-24 苏州科为康生物医药科技有限公司 一种间充质干细胞的制备方法和应用
CN117660325B (zh) * 2024-01-31 2024-04-19 苏州科为康生物医药科技有限公司 一种制备脐带血msc的培养基及其方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1416944B1 (en) 2001-08-14 2005-12-14 Medipost, Co., Ltd. Composition for treatment of articular cartilage damage
EP1446015B1 (en) * 2001-10-18 2018-03-14 Lifecell Corporation Remodeling of tissues and organs
ATE365793T1 (de) 2002-02-19 2007-07-15 Medipost Co Ltd Verfahren zur isolierung und kulturexpansion mesenchymaler stamm-/vorläuferzellen aus nabelschnurblut sowie verfahren zur differenzierung von aus nabelschnurblut stammenden mesenchymalen stamm-/vorläuferzellen in unterschiedliche mesenchymgewebe
EP1539934A2 (en) * 2002-07-30 2005-06-15 Tigenix N.V. Compositions comprising muscle progenitor cells and uses thereof
AU2004274861A1 (en) * 2003-06-06 2005-03-31 Wyeth Methods and materials for identifying agents which modulate bone remodeling and agents identified thereby
JP2006055106A (ja) 2004-08-23 2006-03-02 National Institute Of Advanced Industrial & Technology ヒト血清培地を用いるヒト骨髄由来間葉系幹細胞培養法
CN101395266B (zh) * 2005-12-29 2018-06-15 人类起源公司 胎盘干细胞群
US20070178073A1 (en) * 2006-02-01 2007-08-02 Samsung Life Public Welfare Foundation Composition Comprising Separated or Proliferated Cells from Umbilical Cord Blood for Treating Developmental and/or Chronic Lung Disease
EP2630959A1 (en) * 2007-02-12 2013-08-28 Anthrogenesis Corporation Treatment of inflammatory diseases using placental stem cells
US8114668B2 (en) * 2007-05-14 2012-02-14 Cardiac Pacemakers, Inc. Composition for cold storage of stem cells
WO2009014272A1 (en) * 2007-07-20 2009-01-29 Dongguk University Industry-Academic Cooperation Foundation Method for the preparation of dermal papilla tissue employing mesenchymal stem cells
GB0715648D0 (en) 2007-08-10 2007-09-19 Cambridge Silicon Radio Ltd Channel decoding using similarity matching
ATE479741T1 (de) * 2007-09-21 2010-09-15 Univ Leipzig Zellkulturenmedium und verfahren zur kultivierung von stammzellen und vorläuferzellen
EP2446024A1 (en) * 2009-06-26 2012-05-02 Life&Light Limited Mesenchymal stem cells grown under hypoxic conditions: compositions, methods and uses therefor
KR101243276B1 (ko) * 2009-10-08 2013-03-13 서울대학교병원 인간 지방 줄기세포의 고활성을 유도하는 방법 및 배지
PL2602310T3 (pl) * 2010-08-03 2020-07-13 Sapporo Medical University System hodowli komórek szpiku kostnego zawierających surowicę autologiczną, sposób hodowli komórek szpiku kostnego zawierających surowicę autologiczną i sposób wytwarzania kompozycji leczniczej zawierającej jako składnik aktywny hodowane komórki szpiku kostnego zawierające surowicę autologiczną
US20120052577A1 (en) 2010-08-31 2012-03-01 The Regents Of The University Of California Culture system for stem cell propagation and neural and oligodendrocyte specification

Also Published As

Publication number Publication date
DK2893001T3 (da) 2019-12-02
CA2884000A1 (en) 2014-03-06
IN2015DN01737A (pt) 2015-05-29
US9580687B2 (en) 2017-02-28
KR20140030703A (ko) 2014-03-12
ES2755802T3 (es) 2020-04-23
JP6478243B2 (ja) 2019-03-06
US11332715B2 (en) 2022-05-17
US20150284686A1 (en) 2015-10-08
JP2015532596A (ja) 2015-11-12
CN104781394B (zh) 2017-12-12
EP2893001B1 (en) 2019-10-30
EP2893001A4 (en) 2016-03-09
AU2013309642A1 (en) 2015-03-26
KR101532556B1 (ko) 2015-06-30
US10294456B2 (en) 2019-05-21
US20170191036A1 (en) 2017-07-06
WO2014035215A1 (en) 2014-03-06
CN104781394A (zh) 2015-07-15
CA2884000C (en) 2021-08-24
AU2013309642B2 (en) 2018-10-25
US20190218517A1 (en) 2019-07-18
PL2893001T3 (pl) 2020-03-31
EP2893001A1 (en) 2015-07-15

Similar Documents

Publication Publication Date Title
IL290485A (en) Substrate for the culture of stem cells
EP2893001A4 (en) METHOD OF CULTURING MESENCHYMAL STEM CELLS
HK1199905A1 (en) Culturing of mesenchymal stem cells
EP2774983A4 (en) METHOD OF STEM CELL CULTIVATION
EP2784153A4 (en) METHOD FOR THE CULTURE OF PLURIPOTENT STEM CELLS
HK1208492A1 (en) Highly efficient method for establishing artificial pluripotent stem cell
EP2671945A4 (en) METHOD OF BREEDING HUMAN PLURIPOTENTER STEM CELLS
EP2729561A4 (en) CELL CULTURE METHOD
HK1222200A1 (zh) 製備多能幹細胞的方法
EP2900808A4 (en) METHOD FOR DIFFERENTIATING STEM CELLS IN CHONDROCYTES
GB201117469D0 (en) Culture media for pluripotent stem cells
GB2504996A8 (en) Stem cell culture method
ZA201504388B (en) Method for culturing pluripotent stem cells
EP3041930A4 (en) Method for culturing mesenchymal stem cells according to cell size
SG11201406622UA (en) An in vitro method for culturing stem cells
EP2737064A4 (en) METHOD FOR SCREENING INDUCED PLURIPOTENTAL STEM CELLS
SG11201509767UA (en) Medium for culturing stem cells
EP2686418A4 (en) METHOD FOR THE PRODUCTION OF PLURIPOTENTAL STEM CELLS
EP2559757A4 (en) PROCESS FOR THE PRODUCTION OF INDUCED PLURIPOTENT STEM CELLS
PL2978839T3 (pl) Sposób hodowli komórek
HK1195100A1 (zh) 用於人間充質幹細胞的培養基
SG11201507338PA (en) Method for culturing cells
GB201112292D0 (en) Stem cell culture method
HUP1200079A2 (en) Methods for the characterization of mesenchymal stem cells
SG11201401473RA (en) Method for preparing mesenchymal stem cell-like cells and cardiomyocyte-like cells