SG11201406622UA - An in vitro method for culturing stem cells - Google Patents

An in vitro method for culturing stem cells

Info

Publication number
SG11201406622UA
SG11201406622UA SG11201406622UA SG11201406622UA SG11201406622UA SG 11201406622U A SG11201406622U A SG 11201406622UA SG 11201406622U A SG11201406622U A SG 11201406622UA SG 11201406622U A SG11201406622U A SG 11201406622UA SG 11201406622U A SG11201406622U A SG 11201406622UA
Authority
SG
Singapore
Prior art keywords
stem cells
vitro method
culturing stem
culturing
vitro
Prior art date
Application number
SG11201406622UA
Inventor
Daniele Zink
Ming Ni
Karthikeyan Narayanan
Karthikeyan Kandasamy
Andrew C A Wan
Jackie Y Ying
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG11201406622UA publication Critical patent/SG11201406622UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/815—Dopamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/20—Small organic molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/78—Cellulose
SG11201406622UA 2012-04-20 2013-04-22 An in vitro method for culturing stem cells SG11201406622UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261636157P 2012-04-20 2012-04-20
PCT/SG2013/000160 WO2013158048A1 (en) 2012-04-20 2013-04-22 An in vitro method for culturing stem cells

Publications (1)

Publication Number Publication Date
SG11201406622UA true SG11201406622UA (en) 2014-11-27

Family

ID=49383835

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201406622UA SG11201406622UA (en) 2012-04-20 2013-04-22 An in vitro method for culturing stem cells

Country Status (3)

Country Link
US (1) US10287553B2 (en)
SG (1) SG11201406622UA (en)
WO (1) WO2013158048A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017192717A1 (en) * 2016-05-05 2017-11-09 Southwest Research Institute Three-dimensional bioreactor for cell expansion and related applications
CN110177870B (en) 2017-01-20 2023-10-27 新加坡科技研究局 Thermally responsive microcarrier system and use thereof
US11149244B2 (en) 2018-04-04 2021-10-19 Southwest Research Institute Three-dimensional bioreactor for T-cell activation and expansion for immunotherapy
JP7376576B2 (en) 2018-09-24 2023-11-08 サウスウェスト リサーチ インスティテュート three dimensional bioreactor
CN109575338B (en) * 2018-11-19 2021-05-28 暨南大学 Polylactic acid composite membrane with surface modified with ordered chitin whisker layer, and preparation method and application thereof
US11492580B2 (en) 2020-05-12 2022-11-08 Southwest Research Institute Method using a three-dimensional bioprocessor
CN116121184B (en) * 2022-12-17 2023-09-26 广东赛尔生物科技有限公司 Autologous stem cell preparation, preparation method thereof and application thereof in preparation of immunity-improving and organism-repairing composition

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011017050A1 (en) 2009-07-28 2011-02-10 Corning Incorporated Synthetic microcarriers for culturing cells
ES2963295T3 (en) * 2010-07-12 2024-03-26 Univ Southern California Biocompatible substrate to facilitate interconnections between stem cells and target tissues and methods to implant it
KR20130131363A (en) * 2010-11-09 2013-12-03 론차 콜로그네 게엠베하 Method for controlling binding of cells to a substrate
US9447407B2 (en) 2011-02-02 2016-09-20 Agency For Science, Technology And Research Double coating procedure for the membranes of bioartificial kidneys

Also Published As

Publication number Publication date
US20150087057A1 (en) 2015-03-26
WO2013158048A1 (en) 2013-10-24
US10287553B2 (en) 2019-05-14

Similar Documents

Publication Publication Date Title
HRP20190484T1 (en) Cell culture compositions and methods for polypeptide production
EP2774983A4 (en) Method for culturing stem cell
EP2893001A4 (en) Method for culturing mesenchymal stem cells
EP2784153A4 (en) Method for culturing pluripotent stem cell
EP2671945A4 (en) Method for culturing human pluripotent stem cells
EP2667881A4 (en) Methods for generating inner ear cells in vitro
HK1208492A1 (en) Highly efficient method for establishing artificial pluripotent stem cell
EP2729561A4 (en) Cell culture process
EP2904103B8 (en) Method for preparing induced pluripotent stem cells and its applications
EP2843036A4 (en) Cell culture system and cell culture method
SG11201503579RA (en) Cell culture med
SG11201406622UA (en) An in vitro method for culturing stem cells
GB201214704D0 (en) Stem cell culture method
EP2529009A4 (en) Method for stem cell differentiation
EP2725097A4 (en) Cell culture device and cell culture method
EP2905330A4 (en) Cell culturing member, and cell culturing method
EP2737064A4 (en) Method for screening induced pluripotent stem cells
EP2700707A4 (en) Cell culture method and cell culture kit
EP3070159A4 (en) Method and facility for culturing pluripotent stem cells
ZA201504388B (en) Method for culturing pluripotent stem cells
EP2559757A4 (en) Method for producing induced pluripotent stem cells
HUE039701T2 (en) Cell culture method and system
EP2686418A4 (en) Method for making pluripotent stem cells
EP2978839A4 (en) Method for cell culture
PT3069591T (en) Bioreactor for in vitro plant culture