AU2010279913B2 - Method of efficiently establishing induced pluripotent stem cells - Google Patents
Method of efficiently establishing induced pluripotent stem cells Download PDFInfo
- Publication number
- AU2010279913B2 AU2010279913B2 AU2010279913A AU2010279913A AU2010279913B2 AU 2010279913 B2 AU2010279913 B2 AU 2010279913B2 AU 2010279913 A AU2010279913 A AU 2010279913A AU 2010279913 A AU2010279913 A AU 2010279913A AU 2010279913 B2 AU2010279913 B2 AU 2010279913B2
- Authority
- AU
- Australia
- Prior art keywords
- cells
- cell
- nucleic acid
- pcxle
- encodes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/48—Regulators of apoptosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/604—Klf-4
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/606—Transcription factors c-Myc
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/608—Lin28
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
- C12N2506/1361—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from dental pulp or dental follicle stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Transplantation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23240209P | 2009-08-07 | 2009-08-07 | |
| US61/232,402 | 2009-08-07 | ||
| US30730610P | 2010-02-23 | 2010-02-23 | |
| US61/307,306 | 2010-02-23 | ||
| PCT/JP2010/063733 WO2011016588A1 (en) | 2009-08-07 | 2010-08-06 | Method of efficiently establishing induced pluripotent stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2010279913A1 AU2010279913A1 (en) | 2012-03-15 |
| AU2010279913B2 true AU2010279913B2 (en) | 2016-04-28 |
Family
ID=43544479
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2010279913A Active AU2010279913B2 (en) | 2009-08-07 | 2010-08-06 | Method of efficiently establishing induced pluripotent stem cells |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US8900871B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2462225B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP5376478B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR101774206B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN102625837B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2010279913B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2770412C (cg-RX-API-DMAC7.html) |
| ES (1) | ES2726766T3 (cg-RX-API-DMAC7.html) |
| SG (2) | SG178197A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2011016588A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (96)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2933478T3 (es) | 2005-08-03 | 2023-02-09 | Astellas Inst For Regenerative Medicine | Métodos mejorados de reprogramación de células somáticas animales |
| US20110061118A1 (en) * | 2008-03-17 | 2011-03-10 | Helmholtz Zentrum Munchen | Vectors and methods for generating vector-free induced pluripotent stem (ips) cells using site-specific recombination |
| CA2718904C (en) * | 2008-03-17 | 2017-01-03 | The Scripps Research Institute | Combined chemical and genetic approaches for generation of induced pluripotent stem cells |
| CA2695590C (en) | 2008-06-27 | 2018-01-09 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| KR101685209B1 (ko) * | 2008-07-30 | 2016-12-09 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 유도된 다능성 줄기 세포의 효율적인 확립 방법 |
| ES2645869T3 (es) | 2008-12-17 | 2017-12-11 | The Scripps Research Institute | Generación y mantenimiento de células madre |
| EP2401372A4 (en) | 2009-02-27 | 2012-09-19 | Univ Kyoto | NEW NUCLEAR REPROGRAMMING SUBSTANCE |
| KR101774206B1 (ko) | 2009-08-07 | 2017-09-04 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 유도된 다능성 줄기 세포의 효율적인 확립 방법 |
| US8993329B2 (en) | 2009-09-24 | 2015-03-31 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| JP5808331B2 (ja) * | 2009-10-16 | 2015-11-10 | ザ スクリプス リサーチ インスティテュート | 多能性細胞の誘導法 |
| JP5898086B2 (ja) | 2009-11-04 | 2016-04-06 | セルラー ダイナミクス インターナショナル, インコーポレイテッド | 化学物質を用いるエピソームリプログラミング |
| WO2011102531A1 (en) | 2010-02-16 | 2011-08-25 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| WO2011119942A1 (en) * | 2010-03-25 | 2011-09-29 | Vistagen Therapeutics, Inc. | Induction of ips cells using transient episomal vectors |
| ES2624780T3 (es) | 2010-03-31 | 2017-07-17 | The Scripps Research Institute | Reprogramación de células |
| ES2685171T3 (es) | 2010-06-14 | 2018-10-05 | The Scripps Research Institute | Reprogramación de células a un nuevo destino |
| EP2582794B2 (en) | 2010-06-15 | 2024-04-24 | FUJIFILM Cellular Dynamics, Inc. | Generation of induced pluripotent stem cells from small volumes of peripheral blood |
| US9447408B2 (en) | 2010-09-14 | 2016-09-20 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| CA2814713A1 (en) * | 2010-10-14 | 2012-04-19 | University Of Central Florida Research Foundation, Inc. | Cardiac induced pluripotent stem cells and methods of use in repair and regeneration of myocardium |
| KR101975688B1 (ko) | 2010-12-22 | 2019-05-07 | 페이트 세러퓨틱스, 인코포레이티드 | 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼 |
| CN103562376B (zh) * | 2011-04-08 | 2017-12-29 | 国家医疗保健研究所 | 复壮细胞的方法 |
| WO2012137970A1 (ja) | 2011-04-08 | 2012-10-11 | 国立大学法人大阪大学 | 改変ラミニンおよびその利用 |
| US10865383B2 (en) | 2011-07-12 | 2020-12-15 | Lineage Cell Therapeutics, Inc. | Methods and formulations for orthopedic cell therapy |
| WO2013022022A1 (ja) * | 2011-08-08 | 2013-02-14 | 国立大学法人京都大学 | 効率的な人工多能性幹細胞の樹立方法 |
| WO2013074916A1 (en) | 2011-11-18 | 2013-05-23 | Board Of Regents, The University Of Texas System | Car+ t cells genetically modified to eliminate expression of t- cell receptor and/or hla |
| US20130157368A1 (en) * | 2011-12-20 | 2013-06-20 | Advanced Technologies And Regenerative Medicine, Llc | Induced pluripotent stem cells prepared from human kidney-derived cells |
| US20130157365A1 (en) * | 2011-12-20 | 2013-06-20 | Advanced Technologies And Regenerative Medicine, Llc | Induced pluripotent stem cells from human umbilical cord tissue-derived cells |
| US10155929B2 (en) * | 2012-05-13 | 2018-12-18 | Allele Biotechnology & Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
| US10119150B2 (en) * | 2012-05-13 | 2018-11-06 | Allele Biotechnology & Pharmaceuticals, Inc. | Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
| CA2874259C (en) * | 2012-05-23 | 2021-02-09 | Kyoto University | Highly efficient method for establishing induced pluripotent stem cell |
| CN102965393B (zh) * | 2012-11-06 | 2015-08-26 | 上海交通大学 | 人诱导多能干细胞的制备方法及其用途 |
| AU2014212230B2 (en) | 2013-02-01 | 2019-07-04 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Method for generating retinal pigment epithelium (RPE) cells from induced pluripotent stem cells (iPSCs) |
| WO2014192312A1 (ja) * | 2013-05-31 | 2014-12-04 | 国立大学法人 東京医科歯科大学 | 正常な内向きのカリウム電流特性を有するiPS細胞由来心筋モデル細胞 |
| US10738323B2 (en) | 2013-07-12 | 2020-08-11 | Cedars-Sinai Medical Center | Generation of induced pluripotent stem cells from normal human mammary epithelial cells |
| CN103571794B (zh) * | 2013-07-19 | 2016-01-20 | 中国医学科学院血液病医院(血液学研究所) | 一种抑制PUMA功能改善iPS细胞建立功效的方法 |
| CN103740756B (zh) * | 2013-12-25 | 2015-08-05 | 中国农业大学 | 一种可调控删除的非病毒游离载体及其构建方法 |
| CN103725710B (zh) * | 2013-12-25 | 2015-10-28 | 北京济福霖生物技术有限公司 | 一种可自我删除游离载体及其应用 |
| CN103740757B (zh) * | 2014-01-21 | 2016-04-20 | 中国科学院生物物理研究所 | 一种利用重编程制备猪神经干细胞的方法 |
| CN104830906B (zh) | 2014-02-12 | 2018-09-04 | 北京维通达生物技术有限公司 | 一种重编程获得功能性人肝脏实质细胞的方法 |
| KR102670874B1 (ko) | 2014-03-04 | 2024-05-31 | 페이트 세러퓨틱스, 인코포레이티드 | 개선된 재프로그래밍 방법 및 세포 배양 플랫폼 |
| US10344285B2 (en) | 2014-04-09 | 2019-07-09 | Dna2.0, Inc. | DNA vectors, transposons and transposases for eukaryotic genome modification |
| AU2015249371B2 (en) * | 2014-04-24 | 2020-04-30 | Board Of Regents, The University Of Texas System | Application of induced pluripotent stem cells to generate adoptive cell therapy products |
| EP3786285A1 (en) * | 2014-06-05 | 2021-03-03 | Cedars-Sinai Medical Center | A novel and efficient method for reprogramming immortalized lymphoblastoid cell lines to induced pluripotent stem cells |
| WO2016117139A1 (ja) * | 2015-01-20 | 2016-07-28 | タカラバイオ株式会社 | 神経系細胞の製造方法 |
| US10767165B2 (en) * | 2015-01-30 | 2020-09-08 | Centre National De La Recherche Scientifique (Cnrs) | Reprogramming method for producing induced pluripotent stem cells (iPSC) |
| CA2981715A1 (en) | 2015-04-06 | 2016-10-13 | The Board Of Trustees Of The Leland Stanford Junior University | Chemically modified guide rnas for crispr/cas-mediated gene regulation |
| EP3332020B1 (en) | 2015-08-07 | 2023-12-20 | The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone | Method of determining cellular protein homeostasis |
| AU2016321170B2 (en) | 2015-09-08 | 2022-09-01 | Fujifilm Cellular Dynamics | Method for reproducible differentiation of clinical-grade retinal pigment epithelium cells |
| CN108291206B (zh) | 2015-09-08 | 2022-07-08 | 富士胶片细胞动力公司 | 干细胞来源的视网膜色素上皮的基于macs的纯化 |
| LT3359671T (lt) | 2015-10-08 | 2021-12-10 | Dna Twopointo Inc. | Dnr vektoriai, transpozonai ir transpozazės, skirti eukariotinio genomo modifikacijai |
| EP3362570A4 (en) | 2015-10-16 | 2019-03-20 | Fate Therapeutics, Inc. | PLATFORM FOR THE INDUCTION AND MAINTENANCE OF BASIC STATUS PLURIPOTENCE |
| CN108350429B (zh) | 2015-10-20 | 2022-02-25 | 富士胶片细胞动力公司 | 用于将多能干细胞定向分化为免疫细胞的方法 |
| CN109072198B (zh) | 2016-04-22 | 2022-08-26 | 国立大学法人京都大学 | 产多巴胺神经祖细胞的制备方法 |
| CN109641020B (zh) * | 2016-04-29 | 2022-09-02 | 复诺健生物科技加拿大有限公司 | 在癌细胞中具有增强复制的hsv载体 |
| GB201608944D0 (en) * | 2016-05-20 | 2016-07-06 | Ospedale San Raffaele And Fond Telethon | Gene Tharapy |
| US11572545B2 (en) | 2016-06-16 | 2023-02-07 | Cedars-Sinai Medical Center | Efficient method for reprogramming blood to induced pluripotent stem cells |
| US10221395B2 (en) | 2016-06-16 | 2019-03-05 | Cedars-Sinai Medical Center | Efficient method for reprogramming blood to induced pluripotent stem cells |
| CA3029582A1 (en) | 2016-07-01 | 2018-01-04 | Research Development Foundation | Elimination of proliferating cells from stem cell-derived grafts |
| WO2018026723A1 (en) | 2016-08-01 | 2018-02-08 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Human induced pluripotent stem cells for high efficiency genetic engineering |
| ES2985812T3 (es) | 2016-09-09 | 2024-11-07 | The Board Of Trustees Of The Leland Stanfordjunior Univ | Edición genómica de precisión de alto rendimiento |
| WO2018067826A1 (en) | 2016-10-05 | 2018-04-12 | Cellular Dynamics International, Inc. | Generating mature lineages from induced pluripotent stem cells with mecp2 disruption |
| BR112019006910A2 (pt) * | 2016-10-05 | 2019-07-02 | Fujifilm Cellular Dynamics Inc | métodos para diferenciação dirigida de células-tronco pluripotentes para células imunes homozigóticas de hla |
| AU2017359330B2 (en) | 2016-11-09 | 2022-03-10 | The United States Of America, As Represented By The Secretary Department Of Health And Human Services | 3D vascularized human ocular tissue for cell therapy and drug discovery |
| CN110546267B (zh) * | 2017-02-01 | 2024-09-13 | 哈佛学院董事及会员团体 | 提高细胞基因编辑效率的方法 |
| CN106939299B (zh) * | 2017-02-04 | 2022-11-15 | 滨州医学院 | microRNA重编程体细胞为神经干细胞的制备方法及应用 |
| WO2018152120A1 (en) | 2017-02-14 | 2018-08-23 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Methods of engineering human induced pluripotent stem cells to produce liver tissue |
| KR102668891B1 (ko) | 2017-04-18 | 2024-05-29 | 후지필름 셀룰러 다이내믹스, 인코포레이티드 | 항원-특이적 면역 이펙터 세포 |
| WO2018232079A1 (en) | 2017-06-14 | 2018-12-20 | Daley George Q | Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer |
| WO2019204817A1 (en) | 2018-04-20 | 2019-10-24 | FUJIFILM Cellular Dynamics, Inc. | Method for differentiation of ocular cells and use thereof |
| KR20210095885A (ko) | 2018-11-19 | 2021-08-03 | 더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈 | 생분해성 조직 대체 임플란트 및 그의 용도 |
| KR20210096638A (ko) | 2018-11-28 | 2021-08-05 | 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 | 억제성 환경에 대한 기능 및 내성을 증진시키기 위한 면역 세포의 멀티플렉스 게놈 편집 |
| MX2021006393A (es) | 2018-11-29 | 2021-10-13 | Univ Texas | Metodos para expansion ex vivo de celulas exterminadoras naturales y uso de las mismas. |
| CN109679918A (zh) * | 2018-12-25 | 2019-04-26 | 合肥中科干细胞再生医学有限公司 | 一种便捷的人诱导性多能干细胞的制备方法 |
| CN109666650A (zh) * | 2019-01-04 | 2019-04-23 | 北京航空航天大学 | 一种细胞重编程方法 |
| CN109913494A (zh) * | 2019-02-12 | 2019-06-21 | 北京呈诺医学科技有限公司 | 一种新的ips细胞的诱导方法 |
| BR112021022432A2 (pt) | 2019-05-14 | 2021-12-28 | Aleph Farms Ltd | Agregados de células pluripotentes e uso dos mesmos |
| KR20220130158A (ko) | 2020-01-23 | 2022-09-26 | 더 칠드런스 메디칼 센터 코포레이션 | 인간 만능 줄기 세포로부터의 무-간질 t 세포 분화 |
| WO2021149824A1 (ja) * | 2020-01-24 | 2021-07-29 | アイ ピース, インコーポレイテッド | 人工多能性幹細胞の作製方法 |
| CA3180561A1 (en) | 2020-05-29 | 2021-12-02 | Andrew DIAS | Bilayer of retinal pigmented epithelium and photoreceptors and use thereof |
| AU2021280343A1 (en) | 2020-05-29 | 2022-12-08 | FUJIFILM Cellular Dynamics, Inc. | Retinal pigmented epithelium and photoreceptor dual cell aggregates and methods of use thereof |
| JP2023550680A (ja) * | 2020-07-20 | 2023-12-05 | セルリサーチ コーポレイション プライベート リミテッド | 人工多能性幹細胞を作製する方法、人工多能性幹細胞、および人工多能性幹細胞を使用する方法 |
| CN114149974A (zh) * | 2020-09-08 | 2022-03-08 | 是光隽恒(北京)生物科技有限公司 | 诱导间充质干细胞成为多能干细胞的方法 |
| US20230051406A1 (en) | 2020-11-13 | 2023-02-16 | Catamaran Bio, Inc. | Genetically modified natural killer cells and methods of use thereof |
| WO2022115611A1 (en) | 2020-11-25 | 2022-06-02 | Catamaran Bio, Inc. | Cellular therapeutics engineered with signal modulators and methods of use thereof |
| JP2022104813A (ja) | 2020-12-29 | 2022-07-11 | アイ ピース,インコーポレイテッド | リプログラミング因子を導入された細胞の培養方法 |
| GB202101107D0 (en) * | 2021-01-27 | 2021-03-10 | Oxford Genetics Ltd | Method of genome editing |
| CN117881777A (zh) | 2021-05-26 | 2024-04-12 | 富士胶片细胞动力公司 | 防止多能干细胞中基因快速沉默的方法 |
| EP4346928A1 (en) | 2021-05-28 | 2024-04-10 | The United States of America, as represented by The Secretary, Department of Health and Human Services | Biodegradable tissue scaffold with secondary matrix to host weakly adherent cells |
| WO2022251499A1 (en) | 2021-05-28 | 2022-12-01 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods to generate macular, central and peripheral retinal pigment epithelial cells |
| US11884915B2 (en) | 2021-09-10 | 2024-01-30 | Agilent Technologies, Inc. | Guide RNAs with chemical modification for prime editing |
| JP2024531682A (ja) | 2021-09-13 | 2024-08-29 | フジフィルム セルラー ダイナミクス,インコーポレイテッド | コミットされた心臓始原細胞の製造方法 |
| CN113862221B (zh) * | 2021-10-27 | 2024-04-30 | 上海爱萨尔生物科技有限公司 | 外周血单个核细胞的培养液及培养方法 |
| US20240003871A1 (en) | 2022-06-29 | 2024-01-04 | FUJIFILM Cellular Dynamics, Inc. | Ipsc-derived astrocytes and methods of use thereof |
| CN115044590B (zh) * | 2022-06-30 | 2023-08-15 | 昆明理工大学 | p53基因突变体及其表达的蛋白在制备诊断和治疗肥厚型心肌病的药物中的应用 |
| EP4594471A1 (en) | 2022-09-30 | 2025-08-06 | Fujifilm Cellular Dynamics, Inc. | Methods for the production of cardiac fibroblasts |
| WO2024192329A1 (en) | 2023-03-16 | 2024-09-19 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods for producing stable human chondroctyes and their use for promoting cartillage growth and repair |
| US20250362218A1 (en) | 2024-05-21 | 2025-11-27 | FUJIFILM Cellular Dynamics, Inc. | Particle counting and biomass measurements of aggregated cell compositions |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009057831A1 (ja) * | 2007-10-31 | 2009-05-07 | Kyoto University | 核初期化方法 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4937190A (en) | 1987-10-15 | 1990-06-26 | Wisconsin Alumni Research Foundation | Translation enhancer |
| CA2360671A1 (en) | 1999-01-29 | 2000-08-03 | The Board Of Trustees Of The University Of Illinois | P53 inhibitors and therapeutic use of the same |
| WO2000070070A1 (en) | 1999-05-18 | 2000-11-23 | Dnavec Research Inc. | Paramyxoviridae virus vector defective in envelope gene |
| US20060194321A1 (en) | 2005-01-31 | 2006-08-31 | Alan Colman | Directed differentiation of embryonic stem cells and uses thereof |
| US8278104B2 (en) | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
| CN101864392B (zh) | 2005-12-13 | 2016-03-23 | 国立大学法人京都大学 | 核重新编程因子 |
| WO2007080591A2 (en) * | 2006-01-11 | 2007-07-19 | Technion Research & Development Foundation Ltd. | Adult stem cell-derived connective tissue progenitors for tissue engineering |
| CA2684242C (en) | 2007-03-23 | 2019-11-12 | Wisconsin Alumni Research Foundation | Somatic cell reprogramming |
| WO2008144580A2 (en) * | 2007-05-17 | 2008-11-27 | Oregon Health & Science University | Primate stem cells produced by somatic cell nuclear transfer |
| AU2008286249B2 (en) | 2007-12-10 | 2013-10-10 | Kyoto University | Efficient method for nuclear reprogramming |
| US20110061118A1 (en) | 2008-03-17 | 2011-03-10 | Helmholtz Zentrum Munchen | Vectors and methods for generating vector-free induced pluripotent stem (ips) cells using site-specific recombination |
| CN101550406B (zh) | 2008-04-03 | 2016-02-10 | 北京大学 | 制备多潜能干细胞的方法,试剂盒及用途 |
| CA2954948A1 (en) | 2008-06-04 | 2009-12-10 | Cellular Dynamics International, Inc. | Methods for the production of ips cells using non-viral approach |
| CA2969377C (en) * | 2008-06-13 | 2019-12-03 | Whitehead Institute For Biomedical Research | Programming and reprogramming of cells |
| CA2695590C (en) * | 2008-06-27 | 2018-01-09 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| KR101685209B1 (ko) | 2008-07-30 | 2016-12-09 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 유도된 다능성 줄기 세포의 효율적인 확립 방법 |
| US20110201110A1 (en) | 2008-07-31 | 2011-08-18 | Gifu University | Efficient method for establishing induced pluripotent stem cells |
| JP2010273680A (ja) | 2009-05-29 | 2010-12-09 | Kyoto Univ | 初期化因子が除去された人工多能性幹細胞の作製方法 |
| KR101774206B1 (ko) | 2009-08-07 | 2017-09-04 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 유도된 다능성 줄기 세포의 효율적인 확립 방법 |
| WO2011032166A2 (en) | 2009-09-14 | 2011-03-17 | The Johns Hopkins University | Reprogramming blood cells to pluripotent and multipotent stem cells |
| WO2011102531A1 (en) | 2010-02-16 | 2011-08-25 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| WO2011119942A1 (en) | 2010-03-25 | 2011-09-29 | Vistagen Therapeutics, Inc. | Induction of ips cells using transient episomal vectors |
| US8765470B2 (en) | 2010-08-04 | 2014-07-01 | Cellular Dynamics International, Inc. | Reprogramming immortalized B-cells to induced pluripotent stem cells |
| WO2013022022A1 (ja) | 2011-08-08 | 2013-02-14 | 国立大学法人京都大学 | 効率的な人工多能性幹細胞の樹立方法 |
| CA2874259C (en) | 2012-05-23 | 2021-02-09 | Kyoto University | Highly efficient method for establishing induced pluripotent stem cell |
-
2010
- 2010-08-06 KR KR1020127004663A patent/KR101774206B1/ko active Active
- 2010-08-06 ES ES10806584T patent/ES2726766T3/es active Active
- 2010-08-06 EP EP10806584.8A patent/EP2462225B1/en active Active
- 2010-08-06 US US13/389,359 patent/US8900871B2/en active Active
- 2010-08-06 SG SG2012006862A patent/SG178197A1/en unknown
- 2010-08-06 AU AU2010279913A patent/AU2010279913B2/en active Active
- 2010-08-06 CN CN201080035155.9A patent/CN102625837B/zh active Active
- 2010-08-06 JP JP2012523493A patent/JP5376478B2/ja active Active
- 2010-08-06 SG SG10201608797WA patent/SG10201608797WA/en unknown
- 2010-08-06 WO PCT/JP2010/063733 patent/WO2011016588A1/en not_active Ceased
- 2010-08-06 CA CA2770412A patent/CA2770412C/en active Active
-
2014
- 2014-11-04 US US14/533,080 patent/US9404124B2/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009057831A1 (ja) * | 2007-10-31 | 2009-05-07 | Kyoto University | 核初期化方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102625837A (zh) | 2012-08-01 |
| US20150140662A1 (en) | 2015-05-21 |
| US9404124B2 (en) | 2016-08-02 |
| US20120196360A1 (en) | 2012-08-02 |
| US8900871B2 (en) | 2014-12-02 |
| CA2770412A1 (en) | 2011-02-10 |
| JP5376478B2 (ja) | 2013-12-25 |
| WO2011016588A1 (en) | 2011-02-10 |
| CA2770412C (en) | 2018-09-11 |
| SG10201608797WA (en) | 2016-12-29 |
| KR101774206B1 (ko) | 2017-09-04 |
| KR20120047968A (ko) | 2012-05-14 |
| EP2462225A1 (en) | 2012-06-13 |
| EP2462225A4 (en) | 2013-12-18 |
| ES2726766T3 (es) | 2019-10-09 |
| CN102625837B (zh) | 2015-01-14 |
| SG178197A1 (en) | 2012-03-29 |
| JP2013501505A (ja) | 2013-01-17 |
| AU2010279913A1 (en) | 2012-03-15 |
| EP2462225B1 (en) | 2019-03-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2010279913B2 (en) | Method of efficiently establishing induced pluripotent stem cells | |
| US12012615B2 (en) | Method of making induced pluripotent stem cells using p53 inhibitors | |
| US8962331B2 (en) | Method of making induced pluripotent stem cell from adipose stem cells using minicircle DNA vectors | |
| US9447408B2 (en) | Method of efficiently establishing induced pluripotent stem cells | |
| US10077429B2 (en) | Method of efficiently establishing induced pluripotent stem cells | |
| JP5682043B2 (ja) | 安全な多能性幹細胞の選択方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |