AU2009256295A1 - Compositions and methods comprising variant microbial proteases - Google Patents

Compositions and methods comprising variant microbial proteases Download PDF

Info

Publication number
AU2009256295A1
AU2009256295A1 AU2009256295A AU2009256295A AU2009256295A1 AU 2009256295 A1 AU2009256295 A1 AU 2009256295A1 AU 2009256295 A AU2009256295 A AU 2009256295A AU 2009256295 A AU2009256295 A AU 2009256295A AU 2009256295 A1 AU2009256295 A1 AU 2009256295A1
Authority
AU
Australia
Prior art keywords
pct
bpn
sequence
subtilisin
cleaning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2009256295A
Other languages
English (en)
Inventor
Viktor Yuyevich Alekseyev
Luis G. Cascao-Pereira
James T. Kellis Jr.
Alexander Pisarchik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Publication of AU2009256295A1 publication Critical patent/AU2009256295A1/en
Priority to AU2014202897A priority Critical patent/AU2014202897A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681Chemically modified or immobilised enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21062Subtilisin (3.4.21.62)
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10Objects to be cleaned
    • C11D2111/12Soft surfaces, e.g. textile

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
AU2009256295A 2008-06-06 2009-06-03 Compositions and methods comprising variant microbial proteases Abandoned AU2009256295A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2014202897A AU2014202897A1 (en) 2008-06-06 2014-05-28 Compositions and methods comprising variant microbial proteases

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5969508P 2008-06-06 2008-06-06
US61/059,695 2008-06-06
PCT/US2009/046067 WO2009149145A2 (en) 2008-06-06 2009-06-03 Compositions and methods comprising variant microbial proteases

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2014202897A Division AU2014202897A1 (en) 2008-06-06 2014-05-28 Compositions and methods comprising variant microbial proteases

Publications (1)

Publication Number Publication Date
AU2009256295A1 true AU2009256295A1 (en) 2009-12-10

Family

ID=41009802

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2009256294A Abandoned AU2009256294A1 (en) 2008-06-06 2009-06-03 Compositions and methods comprising variant microbial proteases
AU2009256169A Abandoned AU2009256169A1 (en) 2008-06-06 2009-06-03 Compositions and methods comprising variant microbial proteases
AU2009256295A Abandoned AU2009256295A1 (en) 2008-06-06 2009-06-03 Compositions and methods comprising variant microbial proteases

Family Applications Before (2)

Application Number Title Priority Date Filing Date
AU2009256294A Abandoned AU2009256294A1 (en) 2008-06-06 2009-06-03 Compositions and methods comprising variant microbial proteases
AU2009256169A Abandoned AU2009256169A1 (en) 2008-06-06 2009-06-03 Compositions and methods comprising variant microbial proteases

Country Status (13)

Country Link
US (12) US20110256610A1 (enExample)
EP (10) EP3173479A1 (enExample)
JP (8) JP5647976B2 (enExample)
KR (3) KR20110015004A (enExample)
CN (7) CN102057043B (enExample)
AU (3) AU2009256294A1 (enExample)
BR (3) BRPI0915073A2 (enExample)
CA (3) CA2726370A1 (enExample)
DK (2) DK2578680T3 (enExample)
MX (6) MX2010013121A (enExample)
RU (2) RU2010153866A (enExample)
WO (3) WO2009149145A2 (enExample)
ZA (1) ZA201008169B (enExample)

Families Citing this family (132)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4703113B2 (ja) * 2002-01-16 2011-06-15 ジェネンコー・インターナショナル・インク 複数置換プロテアーゼ変異体
US20090060933A1 (en) * 2004-06-14 2009-03-05 Estell David A Proteases producing an altered immunogenic response and methods of making and using the same
EP3173479A1 (en) 2008-06-06 2017-05-31 Danisco US Inc. Compositions and methods comprising variant microbial proteases
CN102209775A (zh) 2008-11-11 2011-10-05 丹尼斯科美国公司 包含一种或多种可组合的突变的芽孢杆菌属枯草杆菌蛋白酶
CA2743047A1 (en) 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising serine protease variants
EA201270174A1 (ru) * 2009-07-16 2012-07-30 Глэксо Груп Лимитед Отдельные связывающиеся вариабельные домены с улучшенными свойствами, направленные против сывороточного альбумина
US20120252106A1 (en) * 2009-09-25 2012-10-04 Novozymes A/S Use of Protease Variants
CN102652175B (zh) 2009-12-09 2016-02-10 宝洁公司 织物和家居护理产品
DK3190183T3 (da) * 2009-12-09 2019-10-14 Danisco Us Inc Sammensætninger og fremgangsmåder omfattende proteasevarianter
US20120067373A1 (en) * 2010-04-15 2012-03-22 Philip Frank Souter Automatic Dishwashing Detergent Composition
MX339810B (es) * 2010-05-06 2016-06-09 The Procter & Gamble Company * Productos de consumo con variantes de la proteasa.
TR201901382T4 (tr) * 2011-05-05 2019-02-21 Danisco Inc Serin proteaz varyantlarını içeren bileşimler ve yöntemler.
WO2012151480A2 (en) * 2011-05-05 2012-11-08 The Procter & Gamble Company Compositions and methods comprising serine protease variants
US20140342433A1 (en) * 2011-11-25 2014-11-20 Novozymes A/S Subtilase Variants and Polynucleotides Encoding Same
WO2013092635A1 (en) * 2011-12-20 2013-06-27 Novozymes A/S Subtilase variants and polynucleotides encoding same
EP2607468A1 (en) * 2011-12-20 2013-06-26 Henkel AG & Co. KGaA Detergent compositions comprising subtilase variants
EP2628785B1 (en) * 2012-02-17 2016-05-18 Henkel AG & Co. KGaA Detergent compositions comprising subtilase variants
CN104114698A (zh) * 2012-02-17 2014-10-22 诺维信公司 枯草杆菌蛋白酶变体以及编码它们的多核苷酸
WO2014099525A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Paenibacillus curdlanolyticus amylase, and methods of use, thereof
DK2935575T3 (en) 2012-12-21 2018-07-23 Danisco Us Inc ALPHA-amylase variants
DK3336183T4 (da) 2013-03-11 2025-06-10 Danisco Us Inc Kombinatoriske alfa-amylasevarianter
US20160108387A1 (en) 2013-05-29 2016-04-21 Danisco Us Inc. Novel metalloproteases
JP2016526880A (ja) 2013-05-29 2016-09-08 ダニスコ・ユーエス・インク 新規メタロプロテアーゼ
EP3260538B1 (en) 2013-05-29 2021-04-14 Danisco US Inc. Novel metalloproteases
WO2014194032A1 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
US10647974B2 (en) 2013-06-27 2020-05-12 Novozymes A/S Subtilase variants and polynucleotides encoding same
WO2014207224A1 (en) 2013-06-27 2014-12-31 Novozymes A/S Subtilase variants and polynucleotides encoding same
EP3653707B1 (en) * 2013-09-12 2025-07-16 Danisco US Inc. Compositions and methods comprising lg12-clade protease variants
CN105705622A (zh) 2013-10-15 2016-06-22 丹尼斯科美国公司 粘土粒料
DK3068879T3 (da) 2013-11-14 2020-03-16 Danisco Us Inc Stabile enzymer opnået ved glykeringsreduktion
CN106029881B (zh) 2013-12-13 2023-01-10 丹尼斯科美国公司 吉氏芽孢杆菌-进化枝的丝氨酸蛋白酶
EP3080262B1 (en) 2013-12-13 2019-02-06 Danisco US Inc. Serine proteases of bacillus species
AU2014366222B2 (en) 2013-12-16 2018-07-19 Nutrition & Biosciences USA 4, Inc. Use of poly alpha-1,3-glucan ethers as viscosity modifiers
AU2014364772B2 (en) 2013-12-18 2018-07-26 Nutrition & Biosciences USA 4, Inc. Cationic poly alpha-1,3-glucan ethers
EP3097174A1 (en) 2014-01-22 2016-11-30 The Procter & Gamble Company Method of treating textile fabrics
EP3097175B1 (en) 2014-01-22 2018-10-17 The Procter and Gamble Company Fabric treatment composition
WO2015112338A1 (en) 2014-01-22 2015-07-30 The Procter & Gamble Company Method of treating textile fabrics
WO2015112339A1 (en) 2014-01-22 2015-07-30 The Procter & Gamble Company Fabric treatment composition
US20150232785A1 (en) 2014-02-14 2015-08-20 E I Du Pont De Nemours And Company Polysaccharides for viscosity modification
MX2016011467A (es) 2014-03-11 2016-11-16 Du Pont Poli alfa-1,3-glucano oxidado como mejorador de detergente.
DK3119884T3 (da) 2014-03-21 2019-10-14 Danisco Us Inc Serinproteaser af bacillus-arter
US9771548B2 (en) 2014-06-19 2017-09-26 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3164486B1 (en) 2014-07-04 2020-05-13 Novozymes A/S Subtilase variants and polynucleotides encoding same
DK3207129T3 (da) 2014-10-17 2020-02-24 Danisco Us Inc Serinproteaser af bacillus-arten
CN107075534A (zh) 2014-10-24 2017-08-18 丹尼斯科美国公司 使用三肽基肽酶制备醇的方法
EP3550017B1 (en) 2014-10-27 2021-07-14 Danisco US Inc. Serine proteases
EP4403631A3 (en) 2014-10-27 2024-10-30 Danisco US Inc. Serine proteases
US20170335306A1 (en) 2014-10-27 2017-11-23 Danisco Us Inc. Serine proteases
DK3212662T3 (da) 2014-10-27 2020-07-20 Danisco Us Inc Serinproteaser
EP3224357A1 (en) 2014-10-27 2017-10-04 Danisco US Inc. Serine proteases of bacillus species
MX2017006377A (es) 2014-11-17 2017-08-21 Procter & Gamble Composiciones de suministro de agentes beneficos.
US10260024B2 (en) 2014-12-04 2019-04-16 Novozymes A/S Liquid cleaning compositions comprising protease variants
EP3690037A1 (en) * 2014-12-04 2020-08-05 Novozymes A/S Subtilase variants and polynucleotides encoding same
US10995304B2 (en) * 2014-12-15 2021-05-04 Novozymes A/S Subtilase variants
US10400230B2 (en) * 2014-12-19 2019-09-03 Novozymes A/S Protease variants and polynucleotides encoding same
AU2015369965B2 (en) 2014-12-23 2020-01-30 Nutrition & Biosciences USA 4, Inc. Enzymatically produced cellulose
EP3268471B1 (en) * 2015-03-12 2019-08-28 Danisco US Inc. Compositions and methods comprising lg12-clade protease variants
JP2018517803A (ja) 2015-04-29 2018-07-05 ザ プロクター アンド ギャンブル カンパニー 布地の処理方法
CN117683589A (zh) 2015-04-29 2024-03-12 宝洁公司 洗涤织物的方法
PL3088502T3 (pl) 2015-04-29 2018-10-31 The Procter & Gamble Company Sposób obróbki tkaniny
ES2682178T3 (es) 2015-04-29 2018-09-19 The Procter & Gamble Company Composición detergente
US20160319225A1 (en) 2015-04-29 2016-11-03 The Procter & Gamble Company Method of treating a fabric
JP7274819B2 (ja) 2015-05-13 2023-05-17 ダニスコ・ユーエス・インク AprL-CLADEプロテアーゼ変異体及びその使用
WO2016201069A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc Low-density enzyme-containing particles
DK3307427T3 (da) 2015-06-09 2023-11-06 Danisco Us Inc Osmotisk sprængnings-kapsler
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
US11499146B2 (en) 2015-06-17 2022-11-15 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
US20180320158A1 (en) 2015-11-05 2018-11-08 Danisco Us Inc. Paenibacillus and bacillus spp. mannanases
WO2017079751A1 (en) 2015-11-05 2017-05-11 Danisco Us Inc Paenibacillus sp. mannanases
EP3374401B1 (en) 2015-11-13 2022-04-06 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
US10876074B2 (en) 2015-11-13 2020-12-29 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
US10844324B2 (en) 2015-11-13 2020-11-24 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
US10731111B2 (en) 2015-11-25 2020-08-04 Conopco, Inc. Liquid laundry detergent composition
WO2017100720A1 (en) 2015-12-09 2017-06-15 Danisco Us Inc. Alpha-amylase combinatorial variants
EP3390625B1 (en) 2015-12-18 2023-09-06 Danisco US Inc. Polypeptides with endoglucanase activity and uses thereof
DE102016205670A1 (de) * 2016-04-06 2017-10-12 Henkel Ag & Co. Kgaa Neue Protease mit verbesserter Waschleistung
CN109715791B (zh) * 2016-05-03 2023-07-14 丹尼斯科美国公司 蛋白酶变体及其用途
MX2018014768A (es) 2016-05-31 2019-03-06 Danisco Us Inc Variantes de proteasa y sus usos.
WO2017219011A1 (en) 2016-06-17 2017-12-21 Danisco Us Inc Protease variants and uses thereof
US20190264138A1 (en) 2016-11-07 2019-08-29 Danisco Us Inc. Laundry detergent composition
US10550443B2 (en) 2016-12-02 2020-02-04 The Procter & Gamble Company Cleaning compositions including enzymes
MX2019006422A (es) 2016-12-02 2019-08-01 Procter & Gamble Composiciones de limpieza que incluyen enzimas.
EP4001389A1 (en) 2016-12-02 2022-05-25 The Procter & Gamble Company Cleaning compositions including enzymes
DK3559227T3 (da) 2016-12-21 2025-05-05 Danisco Us Inc Proteasevarianter og anvendelser deraf
US11946081B2 (en) 2016-12-21 2024-04-02 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
EP3583210B1 (en) 2017-03-15 2021-07-07 Danisco US Inc. Trypsin-like serine proteases and uses thereof
EP3601515A1 (en) 2017-03-31 2020-02-05 Danisco US Inc. Delayed release enzyme formulations for bleach-containing detergents
CN110809624A (zh) 2017-06-30 2020-02-18 丹尼斯科美国公司 低团聚的含酶颗粒
WO2019059804A1 (ru) * 2017-09-25 2019-03-28 Георгий Георгиевич ЧУМБУРИДЗЕ Термостабильная композиция, обладающая антивирусной и антибактериальной активностью и ее использование
CN111373039A (zh) 2017-11-29 2020-07-03 丹尼斯科美国公司 具有改善的稳定性的枯草杆菌蛋白酶变体
MX2020006518A (es) 2017-12-21 2020-10-28 Danisco Us Inc Gránulos de fusión en caliente, que contienen enzimas, que comprenden un desecante termotolerante.
WO2019156670A1 (en) 2018-02-08 2019-08-15 Danisco Us Inc. Thermally-resistant wax matrix particles for enzyme encapsulation
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
EP3844255A1 (en) 2018-08-30 2021-07-07 Danisco US Inc. Enzyme-containing granules
WO2020068486A1 (en) 2018-09-27 2020-04-02 Danisco Us Inc Compositions for medical instrument cleaning
CN111019925B (zh) * 2018-10-10 2022-08-23 上饶市康可得生物科技有限公司 筛选蛋白酶变体的方法以及获得的蛋白酶变体
WO2020073553A1 (zh) * 2018-10-10 2020-04-16 上饶市康可得生物科技有限公司 筛选蛋白酶变体的方法以及获得的蛋白酶变体
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
CN109481336B (zh) * 2018-12-19 2021-10-26 广州立白企业集团有限公司 口腔护理组合物及其应用
WO2020242858A1 (en) 2019-05-24 2020-12-03 Danisco Us Inc Subtilisin variants and methods of use
EP3980517A1 (en) 2019-06-06 2022-04-13 Danisco US Inc. Methods and compositions for cleaning
IT201900023433A1 (it) 2019-12-10 2021-06-10 Lonati Spa Dispositivo prelevatore per operare il prelievo di un manufatto tubolare a maglia da una macchina circolare per maglieria, calzetteria o simile
EP4204553A1 (en) 2020-08-27 2023-07-05 Danisco US Inc. Enzymes and enzyme compositions for cleaning
US20240117275A1 (en) 2021-01-29 2024-04-11 Danisco Us Inc. Compositions for cleaning and methods related thereto
WO2022171667A1 (en) * 2021-02-12 2022-08-18 Fresenius Kabi Ipsum S.R.L. Subtilisin variants and their use
CN112877304B (zh) * 2021-03-15 2022-07-26 合肥师范学院 一种细菌漆酶突变体LacAt及其表达菌株的构建和应用
EP4363565A1 (en) 2021-06-30 2024-05-08 Danisco US Inc. Variant lipases and uses thereof
WO2023034486A2 (en) 2021-09-03 2023-03-09 Danisco Us Inc. Laundry compositions for cleaning
CN117957318A (zh) 2021-09-13 2024-04-30 丹尼斯科美国公司 含有生物活性物质的颗粒
US20250051748A1 (en) 2021-12-16 2025-02-13 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114932A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023168234A1 (en) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
CN114733656B (zh) * 2022-04-25 2024-01-23 江苏泓润生物质能科技有限公司 一种用于预处理后的餐厨垃圾同步分相除渣及除杂系统
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN119487167A (zh) 2022-06-21 2025-02-18 丹尼斯科美国公司 用于清洁的包含具有嗜热菌蛋白酶活性的多肽的组合物和方法
CA3265943A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. DETERGENT COMPOSITIONS AND ASSOCIATED PROCESSES
WO2024050339A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Mannanase variants and methods of use
JP2025529133A (ja) 2022-09-02 2025-09-04 ダニスコ・ユーエス・インク サブチリシン変異体及びそれに関連する方法
CN120303400A (zh) 2022-11-09 2025-07-11 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2024163600A1 (en) * 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
WO2024163584A1 (en) 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
WO2024163695A1 (en) * 2023-02-01 2024-08-08 The Procter & Gamble Company Detergent compositions containing enzymes
WO2024186819A1 (en) 2023-03-06 2024-09-12 Danisco Us Inc. Subtilisin variants and methods of use
CN120813242A (zh) 2023-03-16 2025-10-17 营养与生物科学美国第四公司 用于清洁和臭味控制的短芽孢杆菌发酵物提取物及其用途
WO2025072144A1 (en) * 2023-09-27 2025-04-03 Osprey Biopharmaceuticals, Inc. Polypeptides having human igg cleavage activity
WO2025071996A1 (en) 2023-09-28 2025-04-03 Danisco Us Inc. Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en) 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use
CN117417922B (zh) * 2023-12-13 2024-03-15 北京科为博生物科技有限公司 一种耐高温碱性蛋白酶及其基因和应用
CN120197407B (zh) * 2025-05-27 2025-08-12 南京华洲药业有限公司 一种除草剂悬浮剂分散稳定性预估优化方法及系统

Family Cites Families (124)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1296839A (enExample) 1969-05-29 1972-11-22
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
GR76237B (enExample) 1981-08-08 1984-08-04 Procter & Gamble
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5700676A (en) 1984-05-29 1997-12-23 Genencor International Inc. Modified subtilisins having amino acid alterations
US5411873A (en) * 1984-05-29 1995-05-02 Genencor, Inc. Process for producing heterologous polypeptides
US5972682A (en) 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK154572C (da) 1985-08-07 1989-04-24 Novo Industri As Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler
WO1987000859A1 (en) 1985-08-09 1987-02-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
ES2058119T3 (es) 1986-08-29 1994-11-01 Novo Nordisk As Aditivo detergente enzimatico.
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
WO1988009367A1 (en) 1987-05-29 1988-12-01 Genencor, Inc. Cutinase cleaning composition
ES2076939T3 (es) 1987-08-28 1995-11-16 Novo Nordisk As Lipasa recombinante de humicola y procedimiento para la produccion de lipasas recombinantes de humicola.
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
JP2624859B2 (ja) 1988-01-07 1997-06-25 ノボ‐ノルディスク アクティーゼルスカブ 酵素洗剤
WO1994002618A1 (en) * 1992-07-17 1994-02-03 Gist-Brocades N.V. High alkaline serine proteases
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
US4977252A (en) 1988-03-11 1990-12-11 National Starch And Chemical Investment Holding Corporation Modified starch emulsifier characterized by shelf stability
WO1990009446A1 (en) 1989-02-17 1990-08-23 Plant Genetic Systems N.V. Cutinase
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK316989D0 (da) * 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
ES2227508T3 (es) * 1989-06-26 2005-04-01 Unilever N.V. Composiciones detergentes enzimaticas.
JP3112937B2 (ja) 1990-04-14 2000-11-27 カリ―ヒエミー アクチエンゲゼルシヤフト アルカリ性バチルスーリパーゼ、これをコード化するdna配列およびこのリパーゼを生産するバチルス
US5354559A (en) 1990-05-29 1994-10-11 Grain Processing Corporation Encapsulation with starch hydrolyzate acid esters
US5766898A (en) * 1990-12-05 1998-06-16 Novo Nordisk A/S Proteins with changed epitopes and methods for the production thereof
EP0495257B1 (en) 1991-01-16 2002-06-12 The Procter & Gamble Company Compact detergent compositions with high activity cellulase
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
DE4231726A1 (de) * 1992-09-23 1994-03-24 Cognis Bio Umwelt Mutierte subtilisinartige Serinproteasen
WO1994006915A1 (en) * 1992-09-24 1994-03-31 Genencor International, Inc. Cleaning compositions containing novel alkaline proteases
WO1994012621A1 (en) 1992-12-01 1994-06-09 Novo Nordisk Enhancement of enzyme reactions
US5646101A (en) 1993-01-18 1997-07-08 The Procter & Gamble Company Machine dishwashing detergents containing an oxygen bleach and an anti-tarnishing mixture of a paraffin oil and sequestrant
DK77393D0 (da) 1993-06-29 1993-06-29 Novo Nordisk As Aktivering af enzymer
US5698504A (en) 1993-07-01 1997-12-16 The Procter & Gamble Company Machine dishwashing composition containing oxygen bleach and paraffin oil and benzotriazole compound silver tarnishing inhibitors
US5486303A (en) 1993-08-27 1996-01-23 The Procter & Gamble Company Process for making high density detergent agglomerates using an anhydrous powder additive
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
ES2275265T3 (es) 1993-09-15 2007-06-01 THE PROCTER & GAMBLE COMPANY Variantes de subtilisina bpn con adsorcion reducida e hidrolisis mejorada.
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
WO1995010591A1 (en) * 1993-10-14 1995-04-20 The Procter & Gamble Company Protease-containing cleaning compositions
HU219852B (hu) * 1993-10-14 2001-08-28 The Procter And Gamble Company Proteáz enzimeket tartalmazó fehérítő készítmények
DE4342680A1 (de) 1993-12-15 1995-06-22 Pfeiffer Erich Gmbh & Co Kg Austragvorrichtung für Medien
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US5691295A (en) 1995-01-17 1997-11-25 Cognis Gesellschaft Fuer Biotechnologie Mbh Detergent compositions
ATE512226T1 (de) 1994-02-24 2011-06-15 Henkel Ag & Co Kgaa Verbesserte enzyme und detergentien damit
ES2364774T3 (es) 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
US5686014A (en) 1994-04-07 1997-11-11 The Procter & Gamble Company Bleach compositions comprising manganese-containing bleach catalysts
PT766727E (pt) 1994-06-17 2002-11-29 Genencor Int Composicoes de limpeza contendo enzimas degradantes de paredes de celulas de plantas e seu uso em metodos de limpeza
GB2294268A (en) 1994-07-07 1996-04-24 Procter & Gamble Bleaching composition for dishwasher use
GB9416841D0 (en) * 1994-08-19 1994-10-12 Finnfeeds Int Ltd An enzyme feed additive and animal feed including it
US5879584A (en) 1994-09-10 1999-03-09 The Procter & Gamble Company Process for manufacturing aqueous compositions comprising peracids
US5516448A (en) 1994-09-20 1996-05-14 The Procter & Gamble Company Process for making a high density detergent composition which includes selected recycle streams for improved agglomerate
US5489392A (en) 1994-09-20 1996-02-06 The Procter & Gamble Company Process for making a high density detergent composition in a single mixer/densifier with selected recycle streams for improved agglomerate properties
US5691297A (en) 1994-09-20 1997-11-25 The Procter & Gamble Company Process for making a high density detergent composition by controlling agglomeration within a dispersion index
DK0796317T3 (da) 1994-12-09 2000-06-05 Procter & Gamble Diacylperoxidpartikelholdig sammensætning til automatisk opvask
US5534179A (en) 1995-02-03 1996-07-09 Procter & Gamble Detergent compositions comprising multiperacid-forming bleach activators
US5574005A (en) 1995-03-07 1996-11-12 The Procter & Gamble Company Process for producing detergent agglomerates from high active surfactant pastes having non-linear viscoelastic properties
US5569645A (en) 1995-04-24 1996-10-29 The Procter & Gamble Company Low dosage detergent composition containing optimum proportions of agglomerates and spray dried granules for improved flow properties
CN1192774A (zh) 1995-06-16 1998-09-09 普罗格特-甘布尔公司 包含钴催化剂的自动洗餐具用组合物
US5597936A (en) 1995-06-16 1997-01-28 The Procter & Gamble Company Method for manufacturing cobalt catalysts
US5565422A (en) 1995-06-23 1996-10-15 The Procter & Gamble Company Process for preparing a free-flowing particulate detergent composition having improved solubility
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
US5576282A (en) 1995-09-11 1996-11-19 The Procter & Gamble Company Color-safe bleach boosters, compositions and laundry methods employing same
EP0859828B2 (en) 1995-09-18 2006-06-14 The Procter & Gamble Company Delivery systems
US5879564A (en) 1995-11-14 1999-03-09 Cytec Technology Corp. High performance polymer flocculating agents
MA24136A1 (fr) 1996-04-16 1997-12-31 Procter & Gamble Fabrication d'agents de surface .
KR100561826B1 (ko) * 1996-11-04 2006-03-16 노보자임스 에이/에스 섭틸라제 변종과 조성물
WO1998020116A1 (en) * 1996-11-04 1998-05-14 Novo Nordisk A/S Subtilase variants and compositions
CN1134443C (zh) 1997-03-07 2004-01-14 普罗格特-甘布尔公司 制备交联桥大环化合物的改进方法
US6306812B1 (en) 1997-03-07 2001-10-23 Procter & Gamble Company, The Bleach compositions containing metal bleach catalyst, and bleach activators and/or organic percarboxylic acids
CN1272881A (zh) * 1997-08-29 2000-11-08 诺沃挪第克公司 蛋白酶变体及组合物
RU2252254C2 (ru) * 1997-10-23 2005-05-20 Джененкор Интернэшнл, Инк. Вариант субтилизина bacillus (варианты), кодирующая его днк, экспрессирующий вектор и очищающая композиция
MA24811A1 (fr) * 1997-10-23 1999-12-31 Procter & Gamble Compositions de lavage contenant des variantes de proteases multisubstituees
US5935826A (en) 1997-10-31 1999-08-10 National Starch And Chemical Investment Holding Corporation Glucoamylase converted starch derivatives and their use as emulsifying and encapsulating agents
BR9814236A (pt) 1997-11-21 2000-10-03 Novo Nordisk As Enzima subtilase, sua variante, sequência de dna isolada, vetor de expressão, célula microbiana hospedeira, processo para produzir uma subtilase ou uma variante de subtilase, composição, e, uso de uma subtilase ou de uma variante de subtilase.
US6773907B2 (en) * 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
CA2313950A1 (en) 1997-12-24 1999-07-08 James T. Kellis, Jr. An improved method of assaying for a preferred enzyme and /or preferred detergent composition
US6936249B1 (en) * 1998-04-15 2005-08-30 Genencor International, Inc. Proteins producing an altered immunogenic response and methods of making and using the same
CN101024826B (zh) 1998-06-10 2014-09-03 诺沃奇梅兹有限公司 新的甘露聚糖酶
US6461849B1 (en) * 1998-10-13 2002-10-08 Novozymes, A/S Modified polypeptide
US6376450B1 (en) 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
JP2002531457A (ja) 1998-11-30 2002-09-24 ザ、プロクター、エンド、ギャンブル、カンパニー 架橋テトラアザマクロサイクル類の製造方法
WO2000037599A1 (en) * 1998-12-18 2000-06-29 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having an additional amino acid residue in the active site loop region
ATE371023T1 (de) * 1998-12-18 2007-09-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit einem zusätzlichen aminosaürerest in einer aktiven schleifenregion
JP4768128B2 (ja) * 1998-12-18 2011-09-07 ノボザイムス アクティーゼルスカブ 活性部位ループ領域中に追加のアミノ酸残基を有するサブチラーゼ酵素サブグループi−s1及びi−s2
BR0010738A (pt) * 1999-05-20 2002-02-19 Novozymes As Enzima subtilase do sub gupo i-s1 ou i-s20, enazima subtilase isolada, variante de subtilase, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, hospedeiro microbiano, método para a produção de uma subtilase ou uma variante de subtilase, composição, e, uso de uma subtilase ou uma variante de subtilase
EP1210415A2 (en) * 1999-07-22 2002-06-05 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
CN1237162C (zh) * 2000-02-23 2006-01-18 宝洁公司 具有增强泥土清除益处的液体衣用洗涤剂组合物
ATE500323T1 (de) * 2000-04-03 2011-03-15 Maxygen Inc Subtilisin-variante
US20020192792A1 (en) * 2000-04-28 2002-12-19 Palle Schneider Laccase mutants
EP1309677B2 (en) 2000-08-11 2012-04-11 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6440991B1 (en) 2000-10-02 2002-08-27 Wyeth Ethers of 7-desmethlrapamycin
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
EP1373296B1 (en) * 2001-03-23 2011-10-05 Genencor International, Inc. Proteins producing an altered immunogenic response and methods of making and using the same
EP1446096A1 (en) * 2001-11-13 2004-08-18 The Procter & Gamble Company Topical compositions containing enzymes stabilised with inhibitors
BR0215383A (pt) * 2001-12-31 2006-11-28 Genencor Int proteases que produzem resposta imunogênica alterada e métodos de fabricação e uso das mesmas
WO2003057246A1 (en) * 2001-12-31 2003-07-17 Genencor International, Inc. Proteases producing an altered immunological response and methods of making and using the same
JP4703113B2 (ja) * 2002-01-16 2011-06-15 ジェネンコー・インターナショナル・インク 複数置換プロテアーゼ変異体
AU2003213580A1 (en) * 2002-02-26 2003-09-09 Genencor International, Inc. Subtilisin carlsberg proteins with reduced immunogenicity
TWI319007B (en) * 2002-11-06 2010-01-01 Novozymes As Subtilase variants
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US20040214737A1 (en) * 2003-04-25 2004-10-28 John Billman Foamy composition for pretreatment of stains on fabrics
US7910115B2 (en) * 2003-04-30 2011-03-22 Shigekazu Nakatsugawa Polypeptide absorbable into digestive organs
US8535927B1 (en) 2003-11-19 2013-09-17 Danisco Us Inc. Micrococcineae serine protease polypeptides and compositions thereof
WO2005056782A2 (en) 2003-12-03 2005-06-23 Genencor International, Inc. Perhydrolase
CA2562171A1 (en) 2004-04-08 2005-11-24 Genencor International, Inc. Mutant .alpha.-amylases
DE102004027091A1 (de) * 2004-06-02 2005-12-29 Henkel Kgaa Leistungsverbesserte Alkalische Protease-Varianten und Wasch- und Reinigungsmittel, enthaltend diese leistungsverbesserten Alkalische Protease-Varianten
WO2007006305A1 (en) * 2005-07-08 2007-01-18 Novozymes A/S Subtilase variants
ES2529815T3 (es) 2005-10-12 2015-02-25 The Procter & Gamble Company Uso y producción de metaloproteasa neutra estable en el almacenamiento
US20090215663A1 (en) * 2006-04-20 2009-08-27 Novozymes A/S Savinase variants having an improved wash performance on egg stains
EP2038393A2 (en) * 2006-06-05 2009-03-25 The Procter and Gamble Company Enzyme stabilization
MX2009000537A (es) * 2006-07-18 2009-01-28 Danisco Inc Genencor Div Variantes de proteasa activas sobre un intervalo amplio de temperatura.
EP3173479A1 (en) * 2008-06-06 2017-05-31 Danisco US Inc. Compositions and methods comprising variant microbial proteases
CA2743047A1 (en) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising serine protease variants
DK3190183T3 (da) * 2009-12-09 2019-10-14 Danisco Us Inc Sammensætninger og fremgangsmåder omfattende proteasevarianter
CN102652175B (zh) * 2009-12-09 2016-02-10 宝洁公司 织物和家居护理产品
EP2551335A1 (en) * 2011-07-25 2013-01-30 The Procter & Gamble Company Enzyme stabilized liquid detergent composition
US20130123162A1 (en) * 2011-11-10 2013-05-16 The Procter & Gamble Company Consumer products

Also Published As

Publication number Publication date
EP2947147A3 (en) 2015-12-23
MX2010013101A (es) 2010-12-21
US12448616B2 (en) 2025-10-21
JP2015012864A (ja) 2015-01-22
JP6426391B2 (ja) 2018-11-21
CN103382421A (zh) 2013-11-06
CN104694519A (zh) 2015-06-10
CN102057043A (zh) 2011-05-11
CN102057043B (zh) 2015-03-04
CN102112607B (zh) 2014-08-20
AU2009256294A1 (en) 2009-12-10
EP2297314B1 (en) 2015-04-15
JP2013240327A (ja) 2013-12-05
EP2647701A3 (en) 2013-12-25
CN104694519B (zh) 2023-10-27
BRPI0913623A8 (pt) 2017-10-03
MX2010013121A (es) 2011-01-21
JP2011524166A (ja) 2011-09-01
WO2009149200A2 (en) 2009-12-10
WO2009149144A2 (en) 2009-12-10
RU2010153864A (ru) 2012-07-20
BRPI0913623A2 (pt) 2015-08-04
KR20110020245A (ko) 2011-03-02
ZA201008169B (en) 2012-02-29
JP2011522539A (ja) 2011-08-04
RU2010154103A (ru) 2012-07-20
KR20110015004A (ko) 2011-02-14
WO2009149144A3 (en) 2010-04-15
MX360255B (es) 2018-10-26
AU2009256169A1 (en) 2009-12-10
EP2947147A2 (en) 2015-11-25
US20180051269A1 (en) 2018-02-22
CN102112607A (zh) 2011-06-29
CN103382420A (zh) 2013-11-06
CN103382420B (zh) 2015-10-28
EP3173479A1 (en) 2017-05-31
CA2726451A1 (en) 2009-12-10
US10563189B2 (en) 2020-02-18
MX2010013100A (es) 2010-12-21
US20180223269A1 (en) 2018-08-09
CN104130993A (zh) 2014-11-05
US20110256610A1 (en) 2011-10-20
EP2578679A1 (en) 2013-04-10
CN104130993B (zh) 2018-10-23
CA2726450A1 (en) 2009-12-10
US20150247140A1 (en) 2015-09-03
KR20110015006A (ko) 2011-02-14
MX383345B (es) 2025-03-13
US20150159147A1 (en) 2015-06-11
JP2011522537A (ja) 2011-08-04
WO2009149145A3 (en) 2010-01-28
US20110251073A1 (en) 2011-10-13
EP2647702A3 (en) 2013-12-25
US20240309352A1 (en) 2024-09-19
WO2009149200A3 (en) 2010-07-29
EP2647701A2 (en) 2013-10-09
EP3095859A1 (en) 2016-11-23
EP2578680A1 (en) 2013-04-10
EP2647702A2 (en) 2013-10-09
DK2578680T3 (en) 2016-08-15
EP2297314A2 (en) 2011-03-23
CN102046783A (zh) 2011-05-04
BRPI0915681A2 (pt) 2015-08-04
EP2297315A2 (en) 2011-03-23
CA2726370A1 (en) 2009-12-10
US20200190497A1 (en) 2020-06-18
DK2297314T3 (en) 2015-07-13
MX350188B (es) 2017-08-30
EP2578680B1 (en) 2016-04-27
JP2015211671A (ja) 2015-11-26
US12410420B2 (en) 2025-09-09
WO2009149145A2 (en) 2009-12-10
CN103382421B (zh) 2016-01-20
JP5647976B2 (ja) 2015-01-07
JP2018050618A (ja) 2018-04-05
US20150056682A1 (en) 2015-02-26
JP6067221B2 (ja) 2017-01-25
US20110256609A1 (en) 2011-10-20
RU2541786C2 (ru) 2015-02-20
US20150064767A1 (en) 2015-03-05
JP2013223499A (ja) 2013-10-31
RU2010153866A (ru) 2012-07-20
BRPI0915073A2 (pt) 2019-09-24
US20220025350A1 (en) 2022-01-27
EP2297316A2 (en) 2011-03-23

Similar Documents

Publication Publication Date Title
US12448616B2 (en) Compositions and methods comprising variant microbial proteases
US20200231908A1 (en) Use and production of citrate-stable neutral metalloproteases
WO2010056640A2 (en) Compositions and methods comprising serine protease variants
AU2014202897A1 (en) Compositions and methods comprising variant microbial proteases
AU2014201928A1 (en) Compositions and methods comprising variant microbial proteases

Legal Events

Date Code Title Description
MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted