ATE225801T1 - Verfahren zur modifizierung rekombinanter polypeptide - Google Patents

Verfahren zur modifizierung rekombinanter polypeptide

Info

Publication number
ATE225801T1
ATE225801T1 AT93918187T AT93918187T ATE225801T1 AT E225801 T1 ATE225801 T1 AT E225801T1 AT 93918187 T AT93918187 T AT 93918187T AT 93918187 T AT93918187 T AT 93918187T AT E225801 T1 ATE225801 T1 AT E225801T1
Authority
AT
Austria
Prior art keywords
group
alpha
polypeptide
terminal
amino acid
Prior art date
Application number
AT93918187T
Other languages
English (en)
Inventor
Jay Stout
Fred W Wagner
Thomas R Coolidge
Bart Holmquist
Original Assignee
Bionebraska Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bionebraska Inc filed Critical Bionebraska Inc
Application granted granted Critical
Publication of ATE225801T1 publication Critical patent/ATE225801T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/003—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by transforming the C-terminal amino acid to amides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/006—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1075—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of amino acids or peptide residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4716—Muscle proteins, e.g. myosin, actin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1019—Tetrapeptides with the first amino acid being basic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/18—Kallidins; Bradykinins; Related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT93918187T 1992-07-13 1993-07-13 Verfahren zur modifizierung rekombinanter polypeptide ATE225801T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91279892A 1992-07-13 1992-07-13
PCT/US1993/006591 WO1994001451A2 (en) 1992-07-13 1993-07-13 Method for modification of recombinant polypeptides

Publications (1)

Publication Number Publication Date
ATE225801T1 true ATE225801T1 (de) 2002-10-15

Family

ID=25432466

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93918187T ATE225801T1 (de) 1992-07-13 1993-07-13 Verfahren zur modifizierung rekombinanter polypeptide

Country Status (10)

Country Link
US (1) US5635371A (de)
EP (2) EP0651761B1 (de)
JP (1) JPH07509128A (de)
AT (1) ATE225801T1 (de)
AU (1) AU673190B2 (de)
CA (1) CA2140002A1 (de)
DE (1) DE69332377T2 (de)
DK (1) DK0651761T3 (de)
NZ (1) NZ255041A (de)
WO (1) WO1994001451A2 (de)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2715404B1 (fr) * 1994-01-25 1996-03-15 Biocem Aconitases végétales et acides nucléiques codant pour ces aconitases.
US6693081B2 (en) * 1995-09-26 2004-02-17 Osteopharm Inc. Bone stimulating factor
DE19713393C2 (de) * 1997-04-01 2002-12-05 Apotech Res & Dev Ltd Flip-Gen und Flip-Protein
GB9708918D0 (en) * 1997-05-01 1997-06-25 Ppl Therapeutics Scotland Ltd Methods
US20030207402A1 (en) * 1997-08-22 2003-11-06 Erhard Kopetzki Autocatalytically activatable zymogenic precursors of proteases and their use
GB9720054D0 (en) * 1997-09-19 1997-11-19 Celltech Therapeutics Ltd Biological products
US8283315B2 (en) 1998-08-28 2012-10-09 Lytix Biopharma As Inhibition of tumour growth
GB9818938D0 (en) 1998-08-28 1998-10-21 Alpharma As Bioactive peptides
US6461834B1 (en) * 1998-11-06 2002-10-08 Bionebraska, Inc. Clostripain catalyzed amidation of peptides
US6743600B1 (en) 1999-04-30 2004-06-01 Monsanto Technologies Llc Method of removing N-terminal alanine residues from polypeptides with Aeromonas aminopeptidase
US6794159B1 (en) * 1999-04-30 2004-09-21 Pharmacia Corporation Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase
CA2431964C (en) 2000-12-20 2013-09-10 F. Hoffmann-La Roche Ag Conjugates of erythropoietin (pep) with polyethylene glycol (peg)
CN1255548C (zh) 2001-06-15 2006-05-10 霍夫曼-拉罗奇有限公司 gp41片段的乙酰化
US20030219854A1 (en) * 2002-03-21 2003-11-27 Micrologix Biotech Inc. Methods for producing modified anti-infective peptides
CA2443365C (en) * 2002-11-19 2010-01-12 F. Hoffmann-La Roche Ag Methods for the recombinant production of antifusogenic peptides
CN1997660A (zh) * 2004-04-21 2007-07-11 芝加哥大学 肌球蛋白轻链激酶抑制剂及其使用
JP5219513B2 (ja) 2004-09-23 2013-06-26 バスジーン セラピューティクス，インコーポレイテッド 血管形成及び腫瘍増殖阻害用ポリペプチド化合物
ATE452145T1 (de) * 2005-05-03 2010-01-15 Novetide Ltd Verfahren zur herstellung von peptidderivaten
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
CN101371140B (zh) 2006-03-09 2013-11-13 弗·哈夫曼-拉罗切有限公司 抗药物抗体的测定
KR20090110295A (ko) 2006-11-22 2009-10-21 에드넥서스, 어 브리스톨-마이어스 스퀴브 알＆디 컴파니 Ｉｇｆ-ｉｒ을 포함하는 티로신 키나제 수용체에 대한 공학처리된 단백질에 기반한 표적화 치료제
ES2558928T3 (es) 2007-01-31 2016-02-09 Dana-Farber Cancer Institute, Inc. Péptidos p53 estabilizados y usos de los mismos
EP2111228B1 (de) 2007-02-02 2011-07-20 Bristol-Myers Squibb Company 10Fn3 Domain zur Behandlung von Krankheiten begleitet von unerwünschter Angiogenese
US8592377B2 (en) 2007-03-28 2013-11-26 President And Fellows Of Harvard College Stitched polypeptides
CL2008002054A1 (es) 2007-07-17 2009-05-29 Hoffmann La Roche Metodo para la regeneracion de una columna de cromatografia de intercambio cationico despues de la elusion de eritropoyetina monopeguilada y metodo para obtener una eritropoyetina monopeguilada, incorporando el metodo de regeneracion de la columna de intercambio cationico.
AR067537A1 (es) 2007-07-17 2009-10-14 Hoffmann La Roche Purificacion de polipeptidos pegilados
AR071874A1 (es) 2008-05-22 2010-07-21 Bristol Myers Squibb Co Proteinas de dominio de armazon basadas en fibronectina multivalentes
CA2736141C (en) 2008-09-23 2018-03-20 F. Hoffmann-La Roche Ag Purification of erythropoietin
TWI496582B (zh) 2008-11-24 2015-08-21 必治妥美雅史谷比公司 雙重專一性之ｅｇｆｒ／ｉｇｆｉｒ結合分子
US8614297B2 (en) 2008-12-22 2013-12-24 Hoffmann-La Roche Inc. Anti-idiotype antibody against an antibody against the amyloid β peptide
BR112012019992A2 (pt) 2010-02-11 2020-08-18 F. Hoffmann-La Roche Ag. conjugados de igf-i poli (etileno glicol)
EP2576615B1 (de) 2010-05-26 2016-03-30 Bristol-Myers Squibb Company Auf fibronectin basierende gerüstproteine mit erhöhter stabilität
JP2013535514A (ja) 2010-08-13 2013-09-12 エイルロン セラピューティクス，インコーポレイテッド ペプチド模倣大環状分子
BR112013005890B1 (pt) 2010-09-14 2022-02-08 F Hoffmann-La Roche Ag Método para purificar eritropoietina peguilada
EP2709669A1 (de) 2011-05-17 2014-03-26 Bristol-Myers Squibb Company Verfahren zur aufrechterhaltung der pegylierung von polypeptiden
TW201806968A (zh) 2011-10-18 2018-03-01 艾利倫治療公司 擬肽巨環化合物
CN104053670A (zh) 2011-10-31 2014-09-17 百时美施贵宝公司 具有降低的免疫原性的纤连蛋白结合域
KR20140099277A (ko) 2011-12-19 2014-08-11 에프. 호프만-라 로슈 아게 다중특이적 결합제의 자유 결합 파트너의 검출 방법
CA2859268A1 (en) 2012-02-01 2013-08-08 F. Hoffmann-La Roche Ag Method for the detection of a binding partner of a multispecific binder
WO2013123267A1 (en) 2012-02-15 2013-08-22 Aileron Therapeutics, Inc. Triazole-crosslinked and thioether-crosslinked peptidomimetic macrocycles
CN104159595A (zh) 2012-02-15 2014-11-19 爱勒让治疗公司 拟肽大环化合物
WO2013149219A2 (en) 2012-03-30 2013-10-03 Sorrento Therapeutics Inc. Fully human antibodies that bind to vegfr2
US9238676B2 (en) 2012-05-17 2016-01-19 Ra Pharmaceuticals, Inc. Peptide and peptidomimetic inhibitors
US9844582B2 (en) 2012-05-22 2017-12-19 Massachusetts Institute Of Technology Synergistic tumor treatment with extended-PK IL-2 and therapeutic agents
EP3553086A1 (de) 2012-05-31 2019-10-16 Sorrento Therapeutics Inc. Antigenbindende proteine zur bindung von pd-l1
EP2863946A4 (de) 2012-06-21 2016-04-13 Sorrento Therapeutics Inc Antigenbindende proteine zur bindung von par-2
CA2877814A1 (en) 2012-06-22 2013-12-27 Dingqiu HUANG Antigen binding proteins that bind ccr2
BR112015000665A2 (pt) 2012-07-13 2017-06-27 Hoffmann La Roche método para a determinação da quantidade de um anticorpo
MX2015005244A (es) 2012-11-01 2015-07-14 Aileron Therapeutics Inc Aminoacidos disustituidos y metodos de preparacion y el uso de los mismos.
WO2014120891A2 (en) 2013-02-01 2014-08-07 Bristol-Myers Squibb Company Fibronectin based scaffold proteins
US20170216403A1 (en) 2014-08-12 2017-08-03 Massachusetts Institute Of Technology Synergistic tumor treatment with il-2, a therapeutic antibody, and an immune checkpoint blocker
CA3133162C (en) 2014-08-12 2024-02-13 Massachusetts Institute Of Technology Synergistic tumor treatment with il-2 and integrin-binding-fc-fusion protein
KR20170067729A (ko) 2014-08-22 2017-06-16 소렌토 쎄라퓨틱스, 인코포레이티드 Cxcr3에 결합하는 항원 결합 단백질
AU2015320549A1 (en) 2014-09-24 2017-04-13 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and uses thereof
WO2016071119A1 (en) 2014-11-05 2016-05-12 F. Hoffmann-La Roche Ag Method for the determination of anti-drug antibodies against an effector function suppressed human or humanized drug antibody
BR112017019738A2 (pt) 2015-03-20 2018-05-29 Aileron Therapeutics Inc macrociclos peptidomiméticos e usos dos mesmos
WO2017066525A1 (en) * 2015-10-14 2017-04-20 The Johns Hopkins University Protein bioconjugation method
AU2016344665C1 (en) 2015-10-29 2023-07-27 F. Hoffmann-La Roche Ag Anti-variant Fc-region antibodies and methods of use
EP3408400A4 (de) 2016-01-29 2019-11-27 Heyue Zhou Antigenbindende proteine zur bindung von pd-l1
SG11201811553UA (en) 2016-07-15 2019-01-30 Hoffmann La Roche Method for purifying pegylated erythropoietin
US10350266B2 (en) 2017-01-10 2019-07-16 Nodus Therapeutics, Inc. Method of treating cancer with a multiple integrin binding Fc fusion protein
AU2018207303A1 (en) 2017-01-10 2019-07-25 xCella Biosciences, Inc. Combination tumor treatment with an integrin-binding-Fc fusion protein and immune modulator
EP3684811A2 (de) 2017-08-17 2020-07-29 Massachusetts Institute of Technology Multispezifitätsbinder von cxc-chemokinen und ihre verwendung
CN111741770A (zh) 2017-12-29 2020-10-02 豪夫迈·罗氏有限公司 用于提供聚乙二醇化蛋白质组合物的方法
EP3731872B1 (de) 2017-12-29 2021-11-24 F. Hoffmann-La Roche AG Verfahren zur bereitstellung monopegylierten proteinen
JP7227633B2 (ja) 2017-12-29 2023-02-22 エフ． ホフマン－ラ ロシュ エージー． Ｐｅｇ化タンパク質組成物を提供するための方法
US11235032B2 (en) 2019-01-23 2022-02-01 Massachusetts Institute Of Technology Combination immunotherapy dosing regimen for immune checkpoint blockade
WO2022065052A1 (ja) * 2020-09-24 2022-03-31 株式会社Ｊｖｃケンウッド 捕捉用抗体、固相担体、検出用抗体、固相担体粒子、並びに検出対象物質の測定キット及び測定方法
CN117858905A (zh) 2021-08-19 2024-04-09 豪夫迈·罗氏有限公司 多价抗变体fc区抗体及使用方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4431739A (en) * 1979-11-05 1984-02-14 Genentech, Inc. Transformant bacterial culture capable of expressing heterologous protein
WO1986002077A1 (en) * 1984-10-02 1986-04-10 Meade Harry M Production of streptavidin-like polypeptides
US5166321A (en) * 1985-01-28 1992-11-24 International Genetic Engineering, Inc. Cecropin polypetides with activity against Gram-positive and Gram-negative bacteria
JPS6229997A (ja) * 1985-04-08 1987-02-07 Sankyo Co Ltd Ｃ末端にプロリンアミドを有するペプチドの製法
US4801536A (en) * 1985-10-11 1989-01-31 Genetics Institute, Inc. Method for producing heterologous proteins
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
WO1988002406A2 (en) * 1986-10-02 1988-04-07 Massachusetts Institute Of Technology Methods of regulating metabolic stability of proteins
US4810777A (en) * 1987-03-04 1989-03-07 The United States Of America As Represented By The Department Of Health And Human Services Antimicrobial compounds
AU601273B2 (en) * 1987-03-20 1990-09-06 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
DE3852074D1 (de) * 1987-03-20 1994-12-15 Creative Biomolecules Inc Verfahren zur reinigung von rekombinanten polypeptiden.
EP0394194B1 (de) * 1989-04-17 1993-08-18 Calbiochem-Novabiochem Ag Geschützte Aminosäuren und Verfahren zu ihrer Herstellung
US5137872A (en) * 1989-09-18 1992-08-11 Pitman-Moore, Inc. Growth hormone-releasing factor analogs
US5101059A (en) * 1989-12-05 1992-03-31 Research Corporation Technologies, Inc. Amino acid protecting groups
US5045531A (en) * 1989-12-18 1991-09-03 Magainin Sciences Inc. Wound treatment employing biologically active ion channel forming peptides and proteins
JPH0423995A (ja) * 1990-05-21 1992-01-28 Takara Shuzo Co Ltd トランスペプチデーション用試薬及び方法
US5169935A (en) * 1990-06-20 1992-12-08 The Salk Institute For Biological Studies Method of making peptides
GB9015369D0 (en) * 1990-07-12 1990-08-29 Erba Carlo Spa Amidated fibrinolytic enzymes and their precursors,and processes for their preparation
ZA922746B (en) * 1991-04-26 1992-12-30 Lilly Co Eli Superactive grf analogs
FR2687680A1 (fr) * 1992-02-20 1993-08-27 Centre Nat Rech Scient Procede de marquage de proteines et de peptides par acylation de leur fonction alpha aminee par un reactif comportant une fonction carboxylique activee.

Also Published As

Publication number Publication date
US5635371A (en) 1997-06-03
WO1994001451A3 (en) 1994-03-03
WO1994001451A2 (en) 1994-01-20
AU4772593A (en) 1994-01-31
CA2140002A1 (en) 1994-01-20
EP0651761A1 (de) 1995-05-10
NZ255041A (en) 1996-11-26
DK0651761T3 (da) 2003-02-10
DE69332377D1 (de) 2002-11-14
JPH07509128A (ja) 1995-10-12
AU673190B2 (en) 1996-10-31
EP1170300A1 (de) 2002-01-09
EP0651761B1 (de) 2002-10-09
DE69332377T2 (de) 2003-07-03

Similar Documents

Publication Publication Date Title
ATE225801T1 (de) Verfahren zur modifizierung rekombinanter polypeptide
ATE247173T1 (de) Charakterisierung von polypeptiden
KR970702917A (ko) Lag-3의 가용성 폴리펩타이드 절편 및 생산 방법, 치료 조성물, 항-이디오타입 항체
DE60043626D1 (de) Verfahren zur therapeutischen Verwendung von löslichen rezeptoren BR43X2
WO1995003405A3 (en) Enzymatic method for modification of recombinant polypeptides
NZ232742A (en) Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced
WO1994018332A3 (en) Anthrax toxin fusion proteins and uses thereof
UA40625C2 (uk) Конструкція днк, рекомбінантний експресуючий вектор, спосіб одержання гетерологічного поліпептиду
IL96043A0 (en) Polypeptides complementary to peptides or proteins having an amino acid sequence or nucleotide coding sequence at least partially known
ATE229068T1 (de) Verfahren zur produktion von polypeptiden
IL90832A0 (en) Gene leader fragment encoding a peptide leader sequence and a polypeptide sequence
ATE253639T1 (de) Verfahren zur herstellung von peptiden
DE69940927D1 (de) PEPTIDFRAGMENTE MIT DEN ZELLTOD VERHINDERNDEr AKTIVITÄT
EP0339217A3 (en) Specific peptide bond cleavage by organic tertiary phosphines with nucleophilic side chains
EP0846950A3 (de) Peptide brauchbar als Mikrosequenzierungsstandard und Verfahren zu derer Anwendung
DE69019077D1 (de) Verfahren zur herstellung von c-terminalen alpha-amidierten peptiden.
NZ238331A (en) Peptide with peptidylhydroxyglycine n-c lyase activity, dna encoding it and its preparation and its use
DE69120409T2 (de) Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte
Facchetti et al. RP-HPLC peptide mapping methods for the analysis of recombinant human pro-urokinase
ATE139538T1 (de) Verfahren zur selektiven spaltung von fusionsproteinen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties