DE3852074D1 - Verfahren zur reinigung von rekombinanten polypeptiden. - Google Patents

Verfahren zur reinigung von rekombinanten polypeptiden.

Info

Publication number
DE3852074D1
DE3852074D1 DE3852074T DE3852074T DE3852074D1 DE 3852074 D1 DE3852074 D1 DE 3852074D1 DE 3852074 T DE3852074 T DE 3852074T DE 3852074 T DE3852074 T DE 3852074T DE 3852074 D1 DE3852074 D1 DE 3852074D1
Authority
DE
Germany
Prior art keywords
cleavage site
purification
fused
amino acids
leader sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3852074T
Other languages
English (en)
Inventor
James Huston
Charles Cohen
Peter Keck
David Rueger
Marc Charette
Roberto Crea
Hermann Oppermann
Richard Ridge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Creative Biomolecules Inc
Original Assignee
Creative Biomolecules Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Creative Biomolecules Inc filed Critical Creative Biomolecules Inc
Application granted granted Critical
Publication of DE3852074D1 publication Critical patent/DE3852074D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE3852074T 1987-03-20 1988-03-04 Verfahren zur reinigung von rekombinanten polypeptiden. Expired - Lifetime DE3852074D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2848487A 1987-03-20 1987-03-20
PCT/US1988/000717 WO1988007085A1 (en) 1987-03-20 1988-03-04 Process for the purification of recombinant polypeptides

Publications (1)

Publication Number Publication Date
DE3852074D1 true DE3852074D1 (de) 1994-12-15

Family

ID=21843701

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3852074T Expired - Lifetime DE3852074D1 (de) 1987-03-20 1988-03-04 Verfahren zur reinigung von rekombinanten polypeptiden.

Country Status (7)

Country Link
EP (2) EP0305500B1 (de)
JP (1) JPH02500876A (de)
AT (1) ATE113985T1 (de)
AU (1) AU605291B2 (de)
CA (1) CA1339208C (de)
DE (1) DE3852074D1 (de)
WO (1) WO1988007085A1 (de)

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5869620A (en) * 1986-09-02 1999-02-09 Enzon, Inc. Multivalent antigen-binding proteins
US4946778A (en) * 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US5260203A (en) * 1986-09-02 1993-11-09 Enzon, Inc. Single polypeptide chain binding molecules
WO1988007086A1 (en) * 1987-03-20 1988-09-22 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
EP0335400B1 (de) * 1988-03-30 1994-06-08 Hitachi, Ltd. Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation
US5262322A (en) * 1988-06-24 1993-11-16 Genentech, Inc. Host transformed with yeast gene and ubiquitin/polypeptide fusions
US5156968A (en) * 1988-06-24 1992-10-20 Genentech, Inc. Purified yeast ubiquitin hydrolase
US5108919A (en) * 1988-06-24 1992-04-28 Genentech, Inc. Dna sequences encoding yeast ubiquitin hydrolase
CA2051375C (en) * 1989-05-04 2000-09-12 Dennis J. Henner Process and compositions for the isolation of human relaxin
US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
DE4105480A1 (de) * 1991-02-21 1992-08-27 Boehringer Mannheim Gmbh Verbesserte aktivierung von rekombinanten proteinen
US5837491A (en) * 1991-11-04 1998-11-17 Xoma Corporation Polynucleotides encoding gelonin sequences
US6146850A (en) 1991-11-04 2000-11-14 Xoma Corporation Proteins encoding gelonin sequences
US5621083A (en) * 1991-11-04 1997-04-15 Xoma Corporation Immunotoxins comprising ribosome-inactivating proteins
US6025165A (en) * 1991-11-25 2000-02-15 Enzon, Inc. Methods for producing multivalent antigen-binding proteins
ATE225801T1 (de) * 1992-07-13 2002-10-15 Bionebraska Inc Verfahren zur modifizierung rekombinanter polypeptide
US5656456A (en) * 1992-07-13 1997-08-12 Bionebraska, Inc. Chemical method for selective modification of the N- and/or C-terminal amino acid α-carbon reactive group of a recombinant polypeptide or a portion thereof
AU5670194A (en) * 1992-11-20 1994-06-22 Enzon, Inc. Linker for linked fusion polypeptides
WO1996017941A2 (en) * 1994-12-07 1996-06-13 Bionebraska, Inc. Production of c-terminal amidated peptides from recombinant protein constructs
AU700605B2 (en) * 1994-12-07 1999-01-07 Bionebraska, Inc. Production of peptides using recombinant fusion protein constructs
GB9716790D0 (en) 1997-08-07 1997-10-15 Creative Peptides Sweden Ab Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
KR100620554B1 (ko) 2004-06-05 2006-09-06 한국생명공학연구원 Ｔａｇ-72에 대한 인간화 항체
US7951559B2 (en) 2007-07-25 2011-05-31 E.I. Du Pont De Nemours And Company Recombinant peptide production using a cross-linkable solubility tag
KR101338517B1 (ko) 2011-04-18 2013-12-10 연세대학교 산학협력단 인간 간-카르복실에스터라제 １을 특이적으로 인식하는 단일클론 항체, 상기 항체를 생산하는 하이브리도마 세포주 및 이의 용도
US9758571B2 (en) 2012-07-20 2017-09-12 University—Industry Cooperation Group Of Kyung Hee University Antibody for epitope tagging, hybridoma cell line and uses thereof
US9580479B2 (en) 2012-07-20 2017-02-28 University—Industry Cooperation Group Of Kyung Hee University Peptide tag and uses thereof
KR101453462B1 (ko) 2013-05-16 2014-10-23 앱클론(주) Her2에 특이적으로 결합하는 항체
KR101605421B1 (ko) 2014-03-05 2016-03-23 국립암센터 B 세포 림프종 세포를 특이적으로 인지하는 단일클론항체 및 이의 용도
CA2969402C (en) 2014-12-03 2019-06-11 Samsung Life Public Welfare Foundation Antibody binding to neuropilin 1 and use thereof
KR102462811B1 (ko) 2015-04-27 2022-11-04 에이비온 주식회사 항체에 덴드론이 접합된 면역접합체 및 이의 용도
KR101896882B1 (ko) 2015-11-30 2018-09-11 앱클론(주) Vegfr2에 특이적으로 결합하는 항체
CN109476738A (zh) 2016-02-15 2019-03-15 社会福祉法人三星生命公益财团 抗EGFRvIII的抗体及其用途
US11155641B2 (en) 2016-05-27 2021-10-26 Orum Therapeutics Inc. Cytosol-penetrating antibody and use thereof
EP3467508A4 (de) 2016-06-03 2020-04-29 Samsung Life Public Welfare Foundation Anti-nrp1-antikörper-screening-verfahren
US11199536B2 (en) 2016-06-03 2021-12-14 Aimed Bio Inc. Method for screening antibody using patient-derived tumor spheroids
WO2017209553A2 (ko) 2016-06-03 2017-12-07 사회복지법인 삼성생명공익재단 환자 유래 세포를 이용한 항체 스크리닝 방법
WO2017209554A2 (ko) 2016-06-03 2017-12-07 사회복지법인 삼성생명공익재단 항-nrp1 항체 스크리닝 방법
BR112019002258A2 (pt) 2016-08-05 2019-05-14 Y-Biologics Inc. anticorpo que se liga a pd-1 ou fragmento de ligação ao antígeno do anticorpo, método para produzir o mesmo e composição para prevenir ou tratar câncer
AU2017306507B2 (en) 2016-08-05 2020-05-14 Y-Biologics Inc. Antibody to programmed death-ligand 1 (PD-L1) and use thereof
WO2018026249A1 (ko) 2016-08-05 2018-02-08 주식회사 와이바이오로직스 프로그램화된 세포 사멸 단백질 리간드-1 (pd-l1)에 대한 항체 및 이의 용도
KR101757346B1 (ko) 2017-03-27 2017-07-26 아주대학교산학협력단 항-emap ii 항체 및 이의 용도
KR101970327B1 (ko) 2017-04-13 2019-04-18 고려대학교 산학협력단 검출 신호의 자가 증폭 원리를 이용한 정확, 신속, 편리한 단일 단계 질병 진단 방법
MX2019014316A (es) 2017-05-30 2020-01-27 Chong Kun Dang Pharmaceutical Corp Un nuevo anticuerpo anti-c-met y uso del mismo.
US11306141B2 (en) 2017-09-08 2022-04-19 Y-Biologics Inc. Antibody against human DLK1 and use thereof
RU2750723C1 (ru) 2017-10-20 2021-07-01 Фармабцин Инк. Антитела против vista и их применение
KR102311838B1 (ko) 2017-12-27 2021-10-14 주식회사 파멥신 항-pd-l1 항체 및 이의 용도
KR102275930B1 (ko) 2018-03-14 2021-07-12 (주)알테오젠 Folr1에 특이적으로 결합하는 항체 및 그의 용도
US11945878B2 (en) 2018-04-18 2024-04-02 Abclon Inc. Switch molecule and switchable chimeric antigen receptor
US11591718B2 (en) 2018-04-27 2023-02-28 Aimed Bio Inc. Magnetic-based biopanning method through attachment of magnetic bead to cell
KR102115300B1 (ko) 2018-06-01 2020-05-26 재단법인 목암생명과학연구소 항체 라이브러리 및 이를 이용한 항체 스크리닝 방법
KR20210043623A (ko) 2018-08-10 2021-04-21 주식회사 유틸렉스 Hla-dr에 결합하는 키메라 항원 수용체 및 car-t 세포
KR102353568B1 (ko) 2018-11-14 2022-01-20 주식회사 헬릭스미스 안정성이 향상된 항 c-Met 항체 또는 그의 항원 결합 단편
US20220339291A1 (en) 2019-03-06 2022-10-27 Legochem Biosciences, Inc. Antibody-drug conjugates that target dlk1 and uses thereof
KR102502287B1 (ko) 2020-04-17 2023-02-22 앱클론(주) 항-her2 어피바디 및 이를 스위치 분자로 이용하는 스위처블 키메라 항원 수용체
WO2021246637A1 (ko) 2020-06-01 2021-12-09 주식회사 이노베이션바이오 Cd22에 특이적인 항체 및 이의 용도
EP4163298A4 (de) 2020-06-09 2024-06-12 Kookmin University Industry Academy Cooperation Foundation Antikörper mit spezifischer bindung an grp94 oder antigenbindendes fragment davon und verwendungen davon
KR20220080375A (ko) 2020-12-07 2022-06-14 (주)이노베이션바이오 Cd47에 특이적인 항체 및 이의 용도
JP2024502129A (ja) 2021-01-12 2024-01-17 エスジー メディカル インコーポレイテッド Ｃｄ５５に対する新規抗体及びその用途
WO2022225329A1 (ko) 2021-04-20 2022-10-27 고려대학교 산학협력단 향상된 암세포 사멸효능을 갖는 비대칭 항체
WO2023277281A1 (ko) 2021-06-30 2023-01-05 (주)이노베이션바이오 Cd47에 특이적인 인간화 항체 및 이를 포함하는 cd47 관련 질환의 예방 또는 치료용 약학적 조성물
CN118339181A (zh) 2021-11-05 2024-07-12 首尔大学医院 抵抗素特异性抗体及其用途

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers
BR8207790A (pt) * 1981-07-15 1983-06-21 Kingdon Craig R Gene estrutural de poliproteina precursor da calcitonina humana
EP0108132A1 (de) * 1982-05-06 1984-05-16 Applied Molecular Genetics Inc. Herstellung und expression von genen für urogastron und dessen polypeptidanalogen
EP0207044A1 (de) * 1985-06-20 1986-12-30 Monsanto Company Freisetzung von Peptiden aus Polypeptiden

Also Published As

Publication number Publication date
ATE113985T1 (de) 1994-11-15
WO1988007085A1 (en) 1988-09-22
EP0305500B1 (de) 1994-11-09
EP0305500A1 (de) 1989-03-08
CA1339208C (en) 1997-08-05
JPH02500876A (ja) 1990-03-29
AU605291B2 (en) 1991-01-10
EP0621340A1 (de) 1994-10-26
AU1575088A (en) 1988-10-10

Similar Documents

Publication Publication Date Title
DE3852074D1 (de) Verfahren zur reinigung von rekombinanten polypeptiden.
PT72834A (pt) Method of synthesis within a bacterial host a selected mature protein or polypeptide
ATE42339T1 (de) Polypeptid- und proteinprodukte und verfahren zu deren herstellung und verwendung.
EP0722461A4 (de) Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird
DE3586333D1 (de) Expressionsvektoren fuer hirudin, transformierte zellen und verfahren zur herstellung von hirudin.
KR920701452A (ko) 프라세싱 부위에 인접한 음전하를 띤 아미노산을 함유하는 이스트 프라세싱 시스템
ATE124456T1 (de) Verfahren zur enzymatischen spaltung rekombinanter proteine unter verwendung von iga- proteasen.
EP0150126A3 (en) The synthesis of protein with an identification peptide
PT83065B (pt) Processo para a preparacao de proteinas de fusao
ATE81673T1 (de) Verfahren zur beseitigung von nendaminos|ureresten aus eukaryotpolypeptidanlagen und so erzeugte polypeptide.
FI880017A (fi) Menetelmä haiman erittämän trypsiini-inhibiittorivarianttien valmistamiseksi ja menetelmässä käytettävä DNA, vektori ja isäntäsolu
DE3585078D1 (de) Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindung.
AU8110487A (en) DNA molecules coding for proteins targeted to plant cell walls
SE8204811L (sv) Dna-fragment innefattande en signalsekvens
EA200000202A1 (ru) Рекомбинантная экспрессия c-пептида инсулина
ATE103292T1 (de) Verfahren zur reinigung rekombinanter polypeptide.
ATE21118T1 (de) Verfahren zur umwandlung von praeproinsulinanaloga zu insulinen.
ES2078229T3 (es) Procedimiento para la preparacion de un polipeptido similar a la motilina y su expresion.
ATE78873T1 (de) Verfahren zur selektiven spaltung von fusionsproteinen.
ATE103983T1 (de) Licht-induzierbarer promotor.
ATE105584T1 (de) Biosynthetisches verfahren zur herstellung von chemischen verbindungen.
ATE191007T1 (de) Verfahren zur rekombinanten herstellung von proteinen in der hefe hansenula
ATE192780T1 (de) Verfahren zur rekombinanten herstellung von proteinen in hefe
DE3825118A1 (de) Expression der viral kodierten protease p2a des hrv2
ATE91303T1 (de) Verfahren zur herstellung von peptiden durch spezifische spaltung von gentechnisch gewonnenen fusionsproteinen mit collagenasen.

Legal Events

Date Code Title Description
8332 No legal effect for de