EA200000202A1 - Рекомбинантная экспрессия c-пептида инсулина - Google Patents

Рекомбинантная экспрессия c-пептида инсулина

Info

Publication number
EA200000202A1
EA200000202A1 EA200000202A EA200000202A EA200000202A1 EA 200000202 A1 EA200000202 A1 EA 200000202A1 EA 200000202 A EA200000202 A EA 200000202A EA 200000202 A EA200000202 A EA 200000202A EA 200000202 A1 EA200000202 A1 EA 200000202A1
Authority
EA
Eurasian Patent Office
Prior art keywords
insulin
peptide
expressed
recombinant expression
cleaved
Prior art date
Application number
EA200000202A
Other languages
English (en)
Inventor
Стефан Столь
Матиас Улен
Пер-Оке Нюгрен
Пер Йонассон
Original Assignee
Криэйтив Пептидз Свиден Аб
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Криэйтив Пептидз Свиден Аб filed Critical Криэйтив Пептидз Свиден Аб
Publication of EA200000202A1 publication Critical patent/EA200000202A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Diabetes (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Obesity (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Emergency Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

В настоящем изобретении предлагается способ получения C-пептида инсулина, при котором осуществляют экспрессию в клетке-хозяине мультимерного полипептида, содержащего множественные копии указанного C-пептида инсулина, и расщепляют указанный экспрессированный полипептид с высвобождением единичных копий C-пептида инсулина. Предлагаются также молекулы нуклеиновых кислот, векторы экспрессии и клетки-хозяева для использования в таком способе, а также мультимерный C-пептид инсулина, экспрессированный и расщепленный таким способом.Отчет о международном поиске был опубликован 1999.04.29.
EA200000202A 1997-08-07 1998-08-07 Рекомбинантная экспрессия c-пептида инсулина EA200000202A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9716790.2A GB9716790D0 (en) 1997-08-07 1997-08-07 Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
PCT/GB1998/002382 WO1999007735A2 (en) 1997-08-07 1998-08-07 Recombinant expression of insulin c-peptide

Publications (1)

Publication Number Publication Date
EA200000202A1 true EA200000202A1 (ru) 2001-04-23

Family

ID=10817184

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200000202A EA200000202A1 (ru) 1997-08-07 1998-08-07 Рекомбинантная экспрессия c-пептида инсулина

Country Status (21)

Country Link
US (1) US6558924B1 (ru)
EP (1) EP1001980A1 (ru)
JP (1) JP2001512745A (ru)
KR (1) KR20010022590A (ru)
CN (1) CN1268141A (ru)
AU (1) AU750685B2 (ru)
BG (1) BG104161A (ru)
BR (1) BR9811850A (ru)
CA (1) CA2297382A1 (ru)
EA (1) EA200000202A1 (ru)
EE (1) EE200000072A (ru)
GB (1) GB9716790D0 (ru)
HU (1) HUP0004626A1 (ru)
IL (1) IL134372A0 (ru)
IS (1) IS5368A (ru)
NO (1) NO20000575L (ru)
NZ (1) NZ502450A (ru)
PL (1) PL339085A1 (ru)
SK (1) SK1532000A3 (ru)
TR (1) TR200000288T2 (ru)
WO (1) WO1999007735A2 (ru)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040005669A1 (en) * 1997-08-07 2004-01-08 Stefan Stahl Recombinant expression of insulin C-peptide
FR2810675B1 (fr) * 2000-06-22 2002-09-27 Pf Medicament Construction modifiee en aval du codon d'initiation pour la surexpression de proteines recombinantes
FR2810674A1 (fr) * 2000-06-22 2001-12-28 Pf Medicament Construction modifiee en amont du codon d'initiation pour la surexpression de proteines recombinantes
AU2002233230B2 (en) * 2000-12-20 2007-02-01 F. Hoffmann-La Roche Ag Erythropoietin conjugates
CA2475388A1 (en) * 2002-02-14 2003-08-21 William J. Rutter Chimeric molecules for cleavage in a treated host
ATE459370T1 (de) 2002-11-26 2010-03-15 Biocon Ltd Modifizierte natriuretic verbindungen, konjugate und ihre verwendungen
US20050026826A1 (en) * 2003-01-17 2005-02-03 Margarethe Hoenig Feline proinsulin, insulin and constituent peptides
AU2003244053A1 (en) * 2003-06-03 2005-01-21 Shanghai Centre Of Research And Development Of New Drugs A fusion protein suitable to be expressed high effectively and the production method thereof
KR20070033965A (ko) * 2004-03-10 2007-03-27 크레이톤 유니버시티 에스트로젠 수용체 및 사용 방법
KR20130115086A (ko) 2010-05-17 2013-10-21 세빅스 인코포레이티드 페길화된 c-펩티드
CN104220086A (zh) 2011-11-17 2014-12-17 塞比克斯股份公司 Peg化的c-肽
SG11201405721VA (en) * 2012-03-19 2014-10-30 Madeleine Pharmaceuticals Ρτy Ltd Method of producing a recombinant peptide
US10627415B2 (en) 2015-03-12 2020-04-21 Board Of Trustees Of Michigan State University Compositions and methods for diagnosing, monitoring, and treating an autoimmune disease
CN109705221B (zh) * 2018-12-27 2021-03-09 美康生物科技股份有限公司 C肽免疫原及其单克隆抗体对及该抗体对在c肽磁微粒化学发光免疫试剂中的应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5210872B2 (ru) * 1973-07-14 1977-03-26
GB2068971B (en) 1980-01-30 1983-06-08 Searle & Co Recombinant dna techniques
CA1213537A (en) 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Polypeptide expression method
EP0621340A1 (en) 1987-03-20 1994-10-26 Creative Biomolecules, Inc. Product and process for the production and purification of recombinant polypeptides
US5013653A (en) 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
SE501169C2 (sv) 1988-02-05 1994-11-28 Aake Nygren Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner
US5130236A (en) 1989-12-07 1992-07-14 Eli Lilly And Company Process for producing des(64,65)-proinsulin
US5595887A (en) 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
GB9015825D0 (en) 1990-07-18 1990-09-05 Ciba Geigy Ag In vitro processing of fusion proteins
JPH07108232B2 (ja) 1990-10-09 1995-11-22 エム・ディ・リサーチ株式会社 ペプチド又は蛋白質の製造方法
US5953418A (en) 1995-06-14 1999-09-14 David Hall Providing selective data broadcast receiver addressability
WO1997023639A1 (fr) 1995-12-22 1997-07-03 Toray Industries, Inc. Procede de production de proteines fusionnees biologiquement actives

Also Published As

Publication number Publication date
BG104161A (en) 2000-12-29
CA2297382A1 (en) 1999-02-18
IL134372A0 (en) 2001-04-30
TR200000288T2 (tr) 2000-06-21
HUP0004626A1 (hu) 2001-04-28
PL339085A1 (en) 2000-12-04
BR9811850A (pt) 2000-08-08
AU750685B2 (en) 2002-07-25
EP1001980A1 (en) 2000-05-24
IS5368A (is) 2000-02-01
GB9716790D0 (en) 1997-10-15
SK1532000A3 (en) 2000-09-12
US6558924B1 (en) 2003-05-06
NO20000575L (no) 2000-04-04
KR20010022590A (ko) 2001-03-26
JP2001512745A (ja) 2001-08-28
CN1268141A (zh) 2000-09-27
NZ502450A (en) 2002-10-25
WO1999007735A3 (en) 1999-04-29
AU8640798A (en) 1999-03-01
WO1999007735A2 (en) 1999-02-18
EE200000072A (et) 2000-10-16
NO20000575D0 (no) 2000-02-04

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