NO20000575L - Rekombinant ekspresjon av insulin C-peptid - Google Patents

Rekombinant ekspresjon av insulin C-peptid

Info

Publication number
NO20000575L
NO20000575L NO20000575A NO20000575A NO20000575L NO 20000575 L NO20000575 L NO 20000575L NO 20000575 A NO20000575 A NO 20000575A NO 20000575 A NO20000575 A NO 20000575A NO 20000575 L NO20000575 L NO 20000575L
Authority
NO
Norway
Prior art keywords
insulin
peptide
recombinant expression
polypeptide
multimeric
Prior art date
Application number
NO20000575A
Other languages
English (en)
Other versions
NO20000575D0 (no
Inventor
Stefan Stohl
Mathias Uhlen
Per-Ke Nygren
Per Jonasson
Original Assignee
Creative Peptides Sweden Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Creative Peptides Sweden Ab filed Critical Creative Peptides Sweden Ab
Publication of NO20000575D0 publication Critical patent/NO20000575D0/no
Publication of NO20000575L publication Critical patent/NO20000575L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Diabetes (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Emergency Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Foreliggende oppfinnelse tilveiebringer en fremgangsmåte for fremstilling av et insulin-C-peptid, som omfatter at man i en vertscelle uttrykker et multimert polypeptid omfattende multiple kopier av insulin-C-peptidet, og spalting av det ut- trykte polypeptid under frigjøring av enkeltkopier av insulin-C-peptidet. Det er også tilveiebrakt nukleinsyremolekyler, ekspresjonsvektorer og vertsceller for an- vendelse ved en slik fremgangsmåte, samt det multimere insulin-C-peptid- polypeptid uttrykt og spaltet ved en slik fremgangsmåte.
NO20000575A 1997-08-07 2000-02-04 Rekombinant ekspresjon av insulin C-peptid NO20000575L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9716790.2A GB9716790D0 (en) 1997-08-07 1997-08-07 Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
PCT/GB1998/002382 WO1999007735A2 (en) 1997-08-07 1998-08-07 Recombinant expression of insulin c-peptide

Publications (2)

Publication Number Publication Date
NO20000575D0 NO20000575D0 (no) 2000-02-04
NO20000575L true NO20000575L (no) 2000-04-04

Family

ID=10817184

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20000575A NO20000575L (no) 1997-08-07 2000-02-04 Rekombinant ekspresjon av insulin C-peptid

Country Status (21)

Country Link
US (1) US6558924B1 (no)
EP (1) EP1001980A1 (no)
JP (1) JP2001512745A (no)
KR (1) KR20010022590A (no)
CN (1) CN1268141A (no)
AU (1) AU750685B2 (no)
BG (1) BG104161A (no)
BR (1) BR9811850A (no)
CA (1) CA2297382A1 (no)
EA (1) EA200000202A1 (no)
EE (1) EE200000072A (no)
GB (1) GB9716790D0 (no)
HU (1) HUP0004626A1 (no)
IL (1) IL134372A0 (no)
IS (1) IS5368A (no)
NO (1) NO20000575L (no)
NZ (1) NZ502450A (no)
PL (1) PL339085A1 (no)
SK (1) SK1532000A3 (no)
TR (1) TR200000288T2 (no)
WO (1) WO1999007735A2 (no)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040005669A1 (en) * 1997-08-07 2004-01-08 Stefan Stahl Recombinant expression of insulin C-peptide
FR2810675B1 (fr) * 2000-06-22 2002-09-27 Pf Medicament Construction modifiee en aval du codon d'initiation pour la surexpression de proteines recombinantes
FR2810674A1 (fr) * 2000-06-22 2001-12-28 Pf Medicament Construction modifiee en amont du codon d'initiation pour la surexpression de proteines recombinantes
AU2002233230B2 (en) * 2000-12-20 2007-02-01 F. Hoffmann-La Roche Ag Erythropoietin conjugates
CN101044154A (zh) * 2002-02-14 2007-09-26 威廉·J·鲁特 治疗宿主中切割的嵌合分子
SG159387A1 (en) 2002-11-26 2010-03-30 Biocon Ltd In Modified natriuretic compounds, conjugates, and uses thereof
US20050026826A1 (en) * 2003-01-17 2005-02-03 Margarethe Hoenig Feline proinsulin, insulin and constituent peptides
US7795384B2 (en) * 2003-06-03 2010-09-14 Shanghai Centre Of Research & Development Of New Drugs Fusion protein suitable for high efficiency expression and the production method thereof
KR20070033965A (ko) * 2004-03-10 2007-03-27 크레이톤 유니버시티 에스트로젠 수용체 및 사용 방법
CN103119055A (zh) 2010-05-17 2013-05-22 塞比克斯公司 聚乙二醇化c肽
CA2855770A1 (en) 2011-11-17 2013-05-23 Cebix Ab Pegylated c-peptide
EP2839010B1 (en) * 2012-03-19 2018-04-25 Madeleine Pharmaceuticals Pty Ltd Method of producing a recombinant peptide
US10627415B2 (en) 2015-03-12 2020-04-21 Board Of Trustees Of Michigan State University Compositions and methods for diagnosing, monitoring, and treating an autoimmune disease
CN109705221B (zh) * 2018-12-27 2021-03-09 美康生物科技股份有限公司 C肽免疫原及其单克隆抗体对及该抗体对在c肽磁微粒化学发光免疫试剂中的应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5210872B2 (no) 1973-07-14 1977-03-26
GB2068971B (en) 1980-01-30 1983-06-08 Searle & Co Recombinant dna techniques
CA1213537A (en) 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Polypeptide expression method
DE3852074D1 (de) 1987-03-20 1994-12-15 Creative Biomolecules Inc Verfahren zur reinigung von rekombinanten polypeptiden.
US5013653A (en) 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
SE501169C2 (sv) 1988-02-05 1994-11-28 Aake Nygren Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner
US5130236A (en) 1989-12-07 1992-07-14 Eli Lilly And Company Process for producing des(64,65)-proinsulin
US5595887A (en) 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
GB9015825D0 (en) 1990-07-18 1990-09-05 Ciba Geigy Ag In vitro processing of fusion proteins
JPH07108232B2 (ja) 1990-10-09 1995-11-22 エム・ディ・リサーチ株式会社 ペプチド又は蛋白質の製造方法
US5953418A (en) 1995-06-14 1999-09-14 David Hall Providing selective data broadcast receiver addressability
CA2213512A1 (en) 1995-12-22 1997-07-03 Toray Industries, Inc. Method for producing bioactive fusion protein

Also Published As

Publication number Publication date
AU8640798A (en) 1999-03-01
WO1999007735A3 (en) 1999-04-29
CA2297382A1 (en) 1999-02-18
BG104161A (en) 2000-12-29
US6558924B1 (en) 2003-05-06
WO1999007735A2 (en) 1999-02-18
EA200000202A1 (ru) 2001-04-23
GB9716790D0 (en) 1997-10-15
PL339085A1 (en) 2000-12-04
KR20010022590A (ko) 2001-03-26
EE200000072A (et) 2000-10-16
HUP0004626A1 (hu) 2001-04-28
IL134372A0 (en) 2001-04-30
NZ502450A (en) 2002-10-25
JP2001512745A (ja) 2001-08-28
EP1001980A1 (en) 2000-05-24
TR200000288T2 (tr) 2000-06-21
BR9811850A (pt) 2000-08-08
IS5368A (is) 2000-02-01
AU750685B2 (en) 2002-07-25
CN1268141A (zh) 2000-09-27
SK1532000A3 (en) 2000-09-12
NO20000575D0 (no) 2000-02-04

Similar Documents

Publication Publication Date Title
NO20000575L (no) Rekombinant ekspresjon av insulin C-peptid
NO20020861L (no) Nye B7-4-molekyler og anvendelser derav
BRPI9811940A (pt) vetor de expressão para um polipeptídeo de ligação da interleucina-18 (il-18-bp), célula hospedeira procariótica transformada com o referido vetor, processo para produção de il-18-bp, proteína de fusão compreendendo o referido polipeptídeo, bem como il-18bp modificada.
BR9813608B1 (pt) célula hospedeira de bacillus transgênica, e, processo para produzir um polipeptìdeo tendo atividade aminopeptidase.
BR0009392A (pt) Polipeptìdeo, sequência de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, métodos para produzir uma sequência de ácido nucleico mutante e para produzir um polipeptìdeo, mutante de um polipeptìdeo com atividade de alafa-amilase, e, uso do polipeptìdeo ou variante
BR9713294A (pt) "anticorpos contra proteìna relacionada a hormÈnio de paratiróide humano"
KR960010864A (ko) 동물 세포의 형질 감염을 위한 재조합 dna 바이러스 벡터
PT728214E (pt) Linhas celulares estaveis capazes de expressar o gene de replicacao do virus adeno-associado
DE68927456D1 (de) RNS-Katalysator zur Spaltung von spezifischen RNS-Sequenzen
EP0776331A4 (en) HIGHER ORDER STRUCTURE AND BINDING OF PEPTIDE NUCLEIC ACIDS
EP1798239A3 (en) G-protein coupled receptors and uses therefor
NO2007008I2 (no) Analoger av humant erytropoietin,DNA-sekvenser somkoder for disse, eukaryot vertscelle transfektert med DNA-sekvensene, preparat som omfatter analoge ne, anvendelse av analogene til økning av hematokritt, og fremgangsmåte for fremstilling av analogene.
BR9915821A (pt) Métodos para transformação de plantas para expressar delta-endotoxinas de bacillus thuringiensis
AU7599500A (en) Novel gl50 molecules and uses therefor
Pekosz et al. Influenza C virus CM2 integral membrane glycoprotein is produced from a polypeptide precursor by cleavage of an internal signal sequence
DE69933550D1 (de) Modifizierte adenoviren welche ein faserersatzprotein enthalten
WO2004083234A3 (en) Insulin polypeptide-oligomer conjugates, proinsulin polypeptide-oligomer conjugates and methods of synthesizing same
BR9811865A (pt) Isolamento de um novo gene de fator de senescência, p23
ID27206A (id) Generasi produk-produk perbanyakan gena dari protein-protein fusi simerik melalui pemutusan endoprotease ubikuitus
AR012499A1 (es) Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombin
WO1996007739A3 (en) Interleukin-1 type 3 receptors
NO972063L (no) Isolert BMP-reseptorkinaseprotein, DNA-sekvens, rekombinant ekspresjonsvektor samt vertscelle
MX9206265A (es) Procedimiento biosintetico para la preparacion de compuestos quimicos.
KR950701345A (ko) 엘라스타제 저해활성을 갖는 신규 펩티드 및 그의 제조방법
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor

Legal Events

Date Code Title Description
FC2A Withdrawal, rejection or dismissal of laid open patent application