ATE113985T1 - Verfahren zur reinigung von rekombinanten polypeptiden. - Google Patents

Verfahren zur reinigung von rekombinanten polypeptiden.

Info

Publication number
ATE113985T1
ATE113985T1 AT88903520T AT88903520T ATE113985T1 AT E113985 T1 ATE113985 T1 AT E113985T1 AT 88903520 T AT88903520 T AT 88903520T AT 88903520 T AT88903520 T AT 88903520T AT E113985 T1 ATE113985 T1 AT E113985T1
Authority
AT
Austria
Prior art keywords
cleavage site
purification
fused
amino acids
leader sequence
Prior art date
Application number
AT88903520T
Other languages
English (en)
Inventor
James S Huston
Charles M Cohen
Peter C Keck
David C Rueger
Marc F Charette
Roberto Crea
Hermann Oppermann
Richard J Ridge
Original Assignee
Creative Biomolecules Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Creative Biomolecules Inc filed Critical Creative Biomolecules Inc
Application granted granted Critical
Publication of ATE113985T1 publication Critical patent/ATE113985T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF] (urogastrone)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
AT88903520T 1987-03-20 1988-03-04 Verfahren zur reinigung von rekombinanten polypeptiden. ATE113985T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2848487A 1987-03-20 1987-03-20

Publications (1)

Publication Number Publication Date
ATE113985T1 true ATE113985T1 (de) 1994-11-15

Family

ID=21843701

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88903520T ATE113985T1 (de) 1987-03-20 1988-03-04 Verfahren zur reinigung von rekombinanten polypeptiden.

Country Status (7)

Country Link
EP (2) EP0305500B1 (de)
JP (1) JPH02500876A (de)
AT (1) ATE113985T1 (de)
AU (1) AU605291B2 (de)
CA (1) CA1339208C (de)
DE (1) DE3852074D1 (de)
WO (1) WO1988007085A1 (de)

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US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
DE4105480A1 (de) * 1991-02-21 1992-08-27 Boehringer Mannheim Gmbh Verbesserte aktivierung von rekombinanten proteinen
US6146850A (en) * 1991-11-04 2000-11-14 Xoma Corporation Proteins encoding gelonin sequences
US5837491A (en) * 1991-11-04 1998-11-17 Xoma Corporation Polynucleotides encoding gelonin sequences
US5621083A (en) * 1991-11-04 1997-04-15 Xoma Corporation Immunotoxins comprising ribosome-inactivating proteins
US6025165A (en) * 1991-11-25 2000-02-15 Enzon, Inc. Methods for producing multivalent antigen-binding proteins
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US5656456A (en) * 1992-07-13 1997-08-12 Bionebraska, Inc. Chemical method for selective modification of the N- and/or C-terminal amino acid α-carbon reactive group of a recombinant polypeptide or a portion thereof
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CN109476738A (zh) 2016-02-15 2019-03-15 社会福祉法人三星生命公益财团 抗EGFRvIII的抗体及其用途
US11155641B2 (en) 2016-05-27 2021-10-26 Orum Therapeutics Inc. Cytosol-penetrating antibody and use thereof
WO2017209553A2 (ko) 2016-06-03 2017-12-07 사회복지법인 삼성생명공익재단 환자 유래 세포를 이용한 항체 스크리닝 방법
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WO2017209554A2 (ko) 2016-06-03 2017-12-07 사회복지법인 삼성생명공익재단 항-nrp1 항체 스크리닝 방법
KR101993893B1 (ko) 2016-06-03 2019-07-01 사회복지법인 삼성생명공익재단 항-nrp1 항체 스크리닝 방법
AU2017306507B2 (en) 2016-08-05 2020-05-14 Y-Biologics Inc. Antibody to programmed death-ligand 1 (PD-L1) and use thereof
WO2018026249A1 (ko) 2016-08-05 2018-02-08 주식회사 와이바이오로직스 프로그램화된 세포 사멸 단백질 리간드-1 (pd-l1)에 대한 항체 및 이의 용도
JP6786722B2 (ja) 2016-08-05 2020-11-18 ワイ−バイオロジクス・インコーポレイテッド プログラム化された細胞死蛋白質（ｐｄ−１）に対する新規抗体及びその用途
KR101757346B1 (ko) 2017-03-27 2017-07-26 아주대학교산학협력단 항-emap ii 항체 및 이의 용도
KR101970327B1 (ko) 2017-04-13 2019-04-18 고려대학교 산학협력단 검출 신호의 자가 증폭 원리를 이용한 정확, 신속, 편리한 단일 단계 질병 진단 방법
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KR102281405B1 (ko) 2017-10-20 2021-07-26 주식회사 파멥신 항-vista 항체 및 이의 용도
KR102311838B1 (ko) 2017-12-27 2021-10-14 주식회사 파멥신 항-pd-l1 항체 및 이의 용도
KR102275930B1 (ko) 2018-03-14 2021-07-12 (주)알테오젠 Folr1에 특이적으로 결합하는 항체 및 그의 용도
WO2019203600A1 (ko) 2018-04-18 2019-10-24 앱클론(주) 스위치 분자 및 스위처블 키메라 항원 수용체
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Also Published As

Publication number Publication date
EP0621340A1 (de) 1994-10-26
JPH02500876A (ja) 1990-03-29
AU605291B2 (en) 1991-01-10
DE3852074D1 (de) 1994-12-15
WO1988007085A1 (en) 1988-09-22
AU1575088A (en) 1988-10-10
EP0305500A1 (de) 1989-03-08
EP0305500B1 (de) 1994-11-09
CA1339208C (en) 1997-08-05

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