AR100216A1 - Edición de genes multiplexados - Google Patents
Edición de genes multiplexadosInfo
- Publication number
- AR100216A1 AR100216A1 ARP150101276A ARP150101276A AR100216A1 AR 100216 A1 AR100216 A1 AR 100216A1 AR P150101276 A ARP150101276 A AR P150101276A AR P150101276 A ARP150101276 A AR P150101276A AR 100216 A1 AR100216 A1 AR 100216A1
- Authority
- AR
- Argentina
- Prior art keywords
- cell
- interest
- embryo
- chromosomal dna
- site
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 239000013611 chromosomal DNA Substances 0.000 abstract 7
- 210000001161 mammalian embryo Anatomy 0.000 abstract 7
- 241000251539 Vertebrata <Metazoa> Species 0.000 abstract 6
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 abstract 6
- 230000002068 genetic effect Effects 0.000 abstract 4
- 108010042407 Endonucleases Proteins 0.000 abstract 2
- 102000004533 Endonucleases Human genes 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000005496 tempering Methods 0.000 abstract 2
- 238000010459 TALEN Methods 0.000 abstract 1
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 abstract 1
- 230000001448 gametogenic effect Effects 0.000 abstract 1
- 238000003209 gene knockout Methods 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 230000000920 spermatogeneic effect Effects 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/101—Bovine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/108—Swine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461985327P | 2014-04-28 | 2014-04-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR100216A1 true AR100216A1 (es) | 2016-09-21 |
Family
ID=53190022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP150101276A AR100216A1 (es) | 2014-04-28 | 2015-04-28 | Edición de genes multiplexados |
Country Status (13)
| Country | Link |
|---|---|
| US (4) | US20160029604A1 (enExample) |
| EP (1) | EP3136850A1 (enExample) |
| JP (1) | JP2017513510A (enExample) |
| KR (1) | KR20170003585A (enExample) |
| CN (2) | CN106535630B (enExample) |
| AP (1) | AP2016009588A0 (enExample) |
| AR (1) | AR100216A1 (enExample) |
| AU (1) | AU2015253352A1 (enExample) |
| BR (2) | BR112016024945A2 (enExample) |
| CA (1) | CA2946881A1 (enExample) |
| MX (1) | MX2016014066A (enExample) |
| PH (1) | PH12016502162A1 (enExample) |
| WO (1) | WO2015168125A1 (enExample) |
Families Citing this family (96)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9528124B2 (en) | 2013-08-27 | 2016-12-27 | Recombinetics, Inc. | Efficient non-meiotic allele introgression |
| US10920242B2 (en) | 2011-02-25 | 2021-02-16 | Recombinetics, Inc. | Non-meiotic allele introgression |
| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| KR101566498B1 (ko) * | 2013-11-13 | 2015-11-06 | 건국대학교 산학협력단 | 인터루킨 2 수용체 감마 유전자 적중벡터, 그 벡터가 도입된 면역세포 결핍 형질전환 미니 복제돼지 생산과 그 제조방법 및 활용 |
| US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
| AP2016009588A0 (en) * | 2014-04-28 | 2016-11-30 | Recombinetics Inc | Multiplex gene editing in swine |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| US10716298B2 (en) | 2014-09-23 | 2020-07-21 | Acceligen, Inc. | Materials and methods for producing animals with short hair |
| CA2963693A1 (en) | 2014-10-10 | 2016-04-14 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| US11673928B2 (en) * | 2015-03-03 | 2023-06-13 | Regents Of The University Of Minnesota | Genetically modified pig cells with an inactivated Etv2 gene |
| AU2016288196A1 (en) | 2015-06-30 | 2018-02-01 | Regents Of The University Of Minnesota | Humanized skeletal muscle |
| CN108472318A (zh) * | 2015-06-30 | 2018-08-31 | 明尼苏达大学校董会 | 人源化心脏肌肉 |
| CA2999500A1 (en) | 2015-09-24 | 2017-03-30 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| US20170099813A1 (en) * | 2015-10-07 | 2017-04-13 | Recombinetics, Inc. | Method of generating sterile terminal sires in livestock and animals produced thereby |
| IL294014B2 (en) | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and uses thereof |
| CN105567689B (zh) * | 2016-01-25 | 2019-04-09 | 重庆威斯腾生物医药科技有限责任公司 | CRISPR/Cas9靶向敲除人TCAB1基因及其特异性gRNA |
| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| EP3443086B1 (en) | 2016-04-13 | 2021-11-24 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| ES2979222T3 (es) * | 2016-04-18 | 2024-09-24 | Vertex Pharma | Materiales y métodos para el tratamiento de hemoglobinopatías |
| CN106191061B (zh) * | 2016-07-18 | 2019-06-18 | 暨南大学 | 一种特异靶向人ABCG2基因的sgRNA导向序列及其应用 |
| AU2017306676B2 (en) | 2016-08-03 | 2024-02-22 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
| EP3497214B1 (en) | 2016-08-09 | 2023-06-28 | President and Fellows of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| WO2018057790A1 (en) | 2016-09-21 | 2018-03-29 | Recombinetics, Inc. | Animal models for cardiomyopathy |
| WO2018071868A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
| AU2017362513A1 (en) * | 2016-11-18 | 2019-05-16 | Christopher Bradley | Massively multiplexed homologous template repair for whole-genome replacement |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP4317447A3 (en) * | 2017-02-15 | 2024-05-01 | 2seventy bio, Inc. | Donor repair templates multiplex genome editing |
| WO2018165631A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| CN106987604B (zh) * | 2017-03-29 | 2021-05-28 | 北京希诺谷生物科技有限公司 | 一种制备动脉粥样硬化疾病模型犬的方法 |
| EP3610398A4 (en) * | 2017-03-30 | 2021-02-24 | Monsanto Technology LLC | SYSTEMS AND METHODS FOR USE IN THE IDENTIFICATION OF MULTIPLE GENOME EDITS AND IN THE AGGREGATE EFFECTS FORECAST OF THE IDENTIFIED GENOME EDITS |
| EP3612023A4 (en) | 2017-04-20 | 2021-05-12 | Egenesis, Inc. | Methods for generating genetically modified animals |
| EP3615552A1 (en) * | 2017-04-24 | 2020-03-04 | DuPont Nutrition Biosciences ApS | Methods and compositions of anti-crispr proteins for use in plants |
| CN107974462A (zh) * | 2017-04-28 | 2018-05-01 | 中山大学 | 一种通过延迟基因的表达时间来提高猪瘦肉产量的方法 |
| CN107182940B (zh) * | 2017-05-09 | 2020-05-15 | 中国科学院动物研究所 | 一种抗寒及瘦肉型转基因猪及其制备方法 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| EP3652312A1 (en) | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| CN107937445B (zh) * | 2017-07-25 | 2021-05-04 | 北京希诺谷生物科技有限公司 | 利用体细胞克隆技术制备基因敲除犬的方法 |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| US20190045761A1 (en) | 2017-08-11 | 2019-02-14 | Recombinetics, Inc. | Inducible disease models methods of making them and use in tissue complementation |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| KR102207352B1 (ko) * | 2017-09-29 | 2021-01-26 | 서울대학교산학협력단 | Klotho 유전자 넉아웃 질환모델 동물 및 이의 용도 |
| TW201932478A (zh) * | 2017-10-12 | 2019-08-16 | 美國馬友醫藥教育研究基金會 | 用於基因編輯的方法和材料 |
| WO2019079347A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | USES OF BASIC EDITORS ADENOSINE |
| AU2018378479B2 (en) | 2017-12-05 | 2025-03-13 | Vertex Pharmaceuticals Incorporated | CRISPR-Cas9 modified CD34+ human hematopoietic stem and progenitor cells and uses thereof |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| EP3749767A1 (en) | 2018-02-05 | 2020-12-16 | Vertex Pharmaceuticals Incorporated | Materials and methods for treatment of hemoglobinopathies |
| CN108504685A (zh) * | 2018-03-27 | 2018-09-07 | 宜明细胞生物科技有限公司 | 一种利用CRISPR/Cas9系统同源重组修复IL-2RG缺陷基因的方法 |
| EP3797160A1 (en) | 2018-05-23 | 2021-03-31 | The Broad Institute Inc. | Base editors and uses thereof |
| WO2020014261A1 (en) | 2018-07-09 | 2020-01-16 | The Broad Institute, Inc. | Rna programmable epigenetic rna modifiers and uses thereof |
| KR102158428B1 (ko) | 2018-08-16 | 2020-09-22 | (주)라트바이오 | 인위적 뉴클레아제를 생산하는 형질전환 배아 및 형질전환 동물의 제작 방법 |
| CN110846330B (zh) * | 2018-08-21 | 2021-08-27 | 中国科学院动物研究所 | 一种突变基因及其用于构建蹼足病小型猪模型的用途 |
| CN109321600A (zh) * | 2018-10-19 | 2019-02-12 | 中国农业大学 | 一种培育生产低致敏性牛奶的牛的方法及其应用 |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| CN109735630A (zh) * | 2019-01-05 | 2019-05-10 | 兰州大学 | 绵羊zfy基因单核苷酸多态性标记的检测方法及标记应用 |
| WO2020154500A1 (en) | 2019-01-23 | 2020-07-30 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| CN111484994B (zh) * | 2019-01-29 | 2022-04-19 | 四川大学华西医院 | CRISPR-Cas9特异性敲除猪Fah、Rag2双基因的方法 |
| CN109777834A (zh) * | 2019-02-25 | 2019-05-21 | 中国科学院广州生物医药与健康研究院 | 一种重度免疫缺陷猪模型及其构建方法和应用 |
| BR112021018607A2 (pt) | 2019-03-19 | 2021-11-23 | Massachusetts Inst Technology | Métodos e composições para editar sequências de nucleotídeos |
| EP3956349A1 (en) | 2019-04-17 | 2022-02-23 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US11859213B2 (en) | 2019-05-16 | 2024-01-02 | Regents Of The University Of Minnesota | Development of superior chimerism by hiPSC engineering and embryo aggregation |
| CN110452929B (zh) * | 2019-07-09 | 2021-07-20 | 中山大学 | 一种非嵌合基因编辑猪胚胎模型的构建方法 |
| WO2021011561A1 (en) * | 2019-07-15 | 2021-01-21 | The General Hospital Corporation | Zebrafish deletion and compound mutants and uses thereof |
| CN110305872A (zh) * | 2019-07-17 | 2019-10-08 | 中国农业科学院北京畜牧兽医研究所 | 小型猪2型糖尿病模型的构建方法及应用 |
| WO2021030735A1 (en) * | 2019-08-15 | 2021-02-18 | The Rockefeller University | Crispr genome editing with cell surface display to produce homozygously edited eukaryotic cells |
| CN112538497B (zh) * | 2019-09-20 | 2023-02-28 | 南京启真基因工程有限公司 | CRISPR/Cas9系统及其在构建α、β和α&β地中海贫血模型猪细胞系中的应用 |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| AU2020388387A1 (en) | 2019-11-22 | 2022-06-02 | President And Fellows Of Harvard College | Ionic liquids for drug delivery |
| KR102270145B1 (ko) * | 2019-12-27 | 2021-07-01 | 서울대학교산학협력단 | Btk 넉아웃 질환모델용 돼지 및 이의 용도 |
| WO2021150712A1 (en) * | 2020-01-21 | 2021-07-29 | The Board Of Regents Of The University Of Texas System | Modulating tlr/nf-kb and p53 signaling pathways to enhance interspecies chimerism between evolutionary distant species |
| AU2021267940A1 (en) | 2020-05-08 | 2022-12-08 | President And Fellows Of Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| CN111334529B (zh) * | 2020-05-20 | 2020-09-29 | 中国农业大学 | 一种利用第三代碱基编辑器制备精准blg基因敲除牛的方法 |
| CN111534519B (zh) * | 2020-05-26 | 2021-12-14 | 中国农业科学院北京畜牧兽医研究所 | 识别猪eIF4G1基因的sgRNA及其编码DNA和应用 |
| EP4158041A4 (en) * | 2020-06-02 | 2024-09-25 | Catalent Pharma Solutions, LLC | CELL LINES WITH MULTIPLE DOCKING ZONES FOR GENE INSERTION |
| CN113234722B (zh) * | 2021-04-30 | 2023-03-14 | 肇庆市瑞思元生物科技有限公司 | 利用碱基编辑修复与板层状鱼鳞病相关的tgm1 c607t突变的试剂和方法 |
| CN113583944B (zh) * | 2021-09-15 | 2023-07-21 | 广西壮族自治区水牛研究所 | 激活Wnt/β-catenin信号通路在提高水牛体外胚胎生产效率中的应用 |
| EP4412587A1 (en) | 2021-10-08 | 2024-08-14 | President And Fellows Of Harvard College | Ionic liquids for drug delivery |
| WO2023077360A1 (zh) * | 2021-11-04 | 2023-05-11 | 云南农业大学 | 一种构建重症免疫缺陷和肝损伤双重猪模型的方法及应用 |
| US20250300846A1 (en) * | 2021-12-17 | 2025-09-25 | Laurie Schmelzle | Systems and methods for generating, managing, and displaying non-fungible digital representations of living, biological, and/or eukaryotic organisms |
| CN115976018B (zh) * | 2022-08-26 | 2025-12-30 | 南京启真基因工程有限公司 | 构建abca12基因突变的斑色鱼鳞癣模型猪核移植供体细胞的试剂盒及其应用 |
| CN115341044A (zh) * | 2022-10-19 | 2022-11-15 | 佛山科学技术学院 | 一种利用微生物及其相关snp位点预测猪日增重的方法 |
| CN115786343B (zh) * | 2022-11-17 | 2024-08-30 | 石河子大学 | 一种猪Zfy基因的RNA干扰片段、表达载体及其应用 |
| CN116334087A (zh) * | 2023-05-10 | 2023-06-27 | 山东农业大学 | 特异性靶向猪Pax3基因的sgRNA序列及其应用 |
| CN116790604B (zh) * | 2023-08-18 | 2023-10-27 | 成都中科奥格生物科技有限公司 | 一种sgRNA、CRISPR/Cas9载体及其构建方法和用途 |
| CN118910057B (zh) * | 2024-09-04 | 2025-03-21 | 江苏农牧科技职业学院 | 用于敲除猪RBM20基因部分保守序列的sgRNA组合、猪模型和构建方法 |
| CN119432858A (zh) * | 2025-01-13 | 2025-02-14 | 江苏省农业科学院 | 一种用于精准编辑奶牛BLG基因的sgRNA组合、引物及应用 |
| CN120204361A (zh) * | 2025-03-28 | 2025-06-27 | 中国科学院水生生物研究所 | Hnf4α或含Hnf4α的生物材料在制备抑制细菌和/或病毒产品中的应用 |
Family Cites Families (59)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4873191A (en) | 1981-06-12 | 1989-10-10 | Ohio University | Genetic transformation of zygotes |
| EP0821070A1 (en) | 1996-07-22 | 1998-01-28 | Carelli, Claude Marcel Henri | Pit-1 gene polymorphism and trait selection in animals |
| GB2365445A (en) | 1998-10-12 | 2002-02-20 | Geron Bio Med Ltd | Porcine occcytes with improved developmental competence |
| AU1100201A (en) | 1999-10-28 | 2001-05-08 | Board Of Trustees Of The Leland Stanford Junior University | Methods of in vivo gene transfer using a sleeping beauty transposon system |
| CN100575485C (zh) | 2002-01-23 | 2009-12-30 | 犹他大学研究基金会 | 使用锌指核酸酶的定向染色体诱变 |
| US7511127B2 (en) | 2002-12-31 | 2009-03-31 | Cargill, Incorporated | Compositions, methods and systems for inferring bovine breed |
| US7985739B2 (en) | 2003-06-04 | 2011-07-26 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced sleeping beauty transposon system and methods for using the same |
| WO2005040400A2 (en) | 2003-10-24 | 2005-05-06 | Mmi Genomics, Inc. | Methods and systems for inferring traits to manage non-beef livestock |
| AU2005237494B2 (en) * | 2004-04-22 | 2010-06-10 | Kyowa Hakko Kirin Co., Ltd | Transgenic animals and uses thereof |
| ES2679282T3 (es) * | 2004-10-22 | 2018-08-23 | Revivicor Inc. | Porcinos transgénicos que carecen de cadena ligera de inmunoglobulina endógena |
| WO2007002372A2 (en) | 2005-06-24 | 2007-01-04 | Regents Of The University Of Minnesota | Using cytosine deaminases to diminish retroelement transfer from pigs to humans |
| AU2008305590A1 (en) * | 2007-09-27 | 2009-04-02 | Sangamo Therapeutics, Inc. | Genomic editing in zebrafish using zinc finger nucleases |
| US8309791B2 (en) | 2008-07-16 | 2012-11-13 | Recombinectics, Inc. | Method for producing a transgenic pig using a hyper-methylated transposon |
| US20110030072A1 (en) * | 2008-12-04 | 2011-02-03 | Sigma-Aldrich Co. | Genome editing of immunodeficiency genes in animals |
| US20110023159A1 (en) | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Ovine genome editing with zinc finger nucleases |
| ES2627552T3 (es) * | 2008-12-04 | 2017-07-28 | Sigma Aldrich Company | Edición de genoma en ratas usando nucleasas con dedos de cinc |
| US20110023140A1 (en) | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Rabbit genome editing with zinc finger nucleases |
| KR20100080068A (ko) | 2008-12-31 | 2010-07-08 | 주식회사 툴젠 | 신규한 징크 핑거 뉴클레아제 및 이의 용도 |
| US20120192298A1 (en) | 2009-07-24 | 2012-07-26 | Sigma Aldrich Co. Llc | Method for genome editing |
| CN102858985A (zh) * | 2009-07-24 | 2013-01-02 | 西格马-奥尔德里奇有限责任公司 | 基因组编辑方法 |
| DK2462230T3 (en) | 2009-08-03 | 2015-10-19 | Recombinetics Inc | METHODS AND COMPOSITIONS FOR TARGETED RE-MODIFICATION |
| WO2011020005A1 (en) | 2009-08-14 | 2011-02-17 | Regeneron Pharmaceuticals, Inc. | miRNA-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE |
| DK2510096T4 (en) | 2009-12-10 | 2018-05-14 | Univ Iowa State Res Found Inc | NUMBER EFFECTOR-MEDIATED DNA MODIFICATION |
| AU2011215557B2 (en) * | 2010-02-09 | 2016-03-10 | Sangamo Therapeutics, Inc. | Targeted genomic modification with partially single-stranded donor molecules |
| EP2533629B1 (en) | 2010-02-11 | 2018-11-28 | Recombinetics, Inc. | Methods and materials for producing transgenic artiodactyls |
| JP5841322B2 (ja) | 2010-04-22 | 2016-01-13 | オレゴン ヘルス アンド サイエンス ユニバーシティ | フマリルアセト酢酸ヒドロラーゼ(fah)欠損性ブタおよびその使用 |
| BR112012027150A2 (pt) * | 2010-04-23 | 2015-09-15 | Du Pont | método para regular a expressão em uma célula vegetal, interruptor gênico, planta transgênica, célula vegetal transgênica ou semente transgênica e polinucleotídeo recombinante |
| EP2392208B1 (en) | 2010-06-07 | 2016-05-04 | Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | Fusion proteins comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease and their use |
| CN103228130B (zh) * | 2010-06-17 | 2016-03-16 | 科马布有限公司 | 动物模型及治疗分子 |
| JP2013537410A (ja) | 2010-07-23 | 2013-10-03 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | 標的化エンドヌクレアーゼおよび一本鎖核酸を用いたゲノム編集 |
| CN103930550B (zh) | 2011-02-25 | 2017-02-15 | 重组股份有限公司 | 经遗传修饰的动物及其生成方法 |
| US9528124B2 (en) | 2013-08-27 | 2016-12-27 | Recombinetics, Inc. | Efficient non-meiotic allele introgression |
| SG194089A1 (en) * | 2011-04-27 | 2013-11-29 | Amyris Inc | Methods for genomic modification |
| US20130212722A1 (en) | 2012-02-15 | 2013-08-15 | Mice With Horns, Llc | Transgenic animals with customizable traits |
| US10266850B2 (en) | 2012-05-25 | 2019-04-23 | The Regents Of The University Of California | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| AR091482A1 (es) * | 2012-06-21 | 2015-02-04 | Recombinetics Inc | Celulas modificadas geneticamente y metodos par su obtencion |
| US10058078B2 (en) | 2012-07-31 | 2018-08-28 | Recombinetics, Inc. | Production of FMDV-resistant livestock by allele substitution |
| US20140115728A1 (en) | 2012-10-24 | 2014-04-24 | A. Joseph Tector | Double knockout (gt/cmah-ko) pigs, organs and tissues |
| AU2013337951B2 (en) | 2012-10-30 | 2019-10-03 | Recombinetics, Inc. | Control of sexual maturation in animals |
| EP2914102B1 (en) | 2012-11-05 | 2017-10-18 | Regeneron Pharmaceuticals, Inc. | Genetically modified non-human animals and methods of use thereof |
| CA3034794A1 (en) | 2012-12-06 | 2014-06-12 | Sigma-Aldrich Co. Llc | Crispr-based genome modification and regulation |
| EP4234696A3 (en) | 2012-12-12 | 2023-09-06 | The Broad Institute Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| EP2931891B1 (en) | 2012-12-17 | 2019-05-15 | President and Fellows of Harvard College | Rna-guided human genome engineering |
| US10612043B2 (en) | 2013-03-09 | 2020-04-07 | Agilent Technologies, Inc. | Methods of in vivo engineering of large sequences using multiple CRISPR/cas selections of recombineering events |
| US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
| US20160053274A1 (en) | 2013-04-02 | 2016-02-25 | Bayer Cropscience Nv | Targeted genome engineering in eukaryotes |
| US20150013025A1 (en) | 2013-05-19 | 2015-01-08 | Mice With Horns, Llc | Transgenic animals with customizable traits |
| US20140359795A1 (en) | 2013-05-31 | 2014-12-04 | Recombinetics, Inc. | Genetic techniques for making animals with sortable sperm |
| US20140359796A1 (en) | 2013-05-31 | 2014-12-04 | Recombinetics, Inc. | Genetically sterile animals |
| CN105658805B (zh) | 2013-06-05 | 2021-12-31 | 杜克大学 | Rna指导的基因编辑和基因调节 |
| KR20220025922A (ko) | 2013-07-09 | 2022-03-03 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 멀티플렉스 rna-가이드된 게놈 조작 |
| US9670458B2 (en) | 2013-07-13 | 2017-06-06 | Aggenetics, Inc. | Methods for spermatogonial stem cell (SSC) transfer |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US20150064149A1 (en) | 2013-09-04 | 2015-03-05 | Mice With Horns, LLC. | Materials and methods for correcting recessive mutations in animals |
| WO2015052231A2 (en) | 2013-10-08 | 2015-04-16 | Technical University Of Denmark | Multiplex editing system |
| US10787684B2 (en) | 2013-11-19 | 2020-09-29 | President And Fellows Of Harvard College | Large gene excision and insertion |
| DE212015000061U1 (de) | 2014-02-11 | 2017-09-03 | The Regents Of The University Of Colorado, A Body Corporate | CRISPR-ermöglichtes Multiplex Genom Engineering |
| AP2016009588A0 (en) * | 2014-04-28 | 2016-11-30 | Recombinetics Inc | Multiplex gene editing in swine |
-
2015
- 2015-04-28 AP AP2016009588A patent/AP2016009588A0/en unknown
- 2015-04-28 JP JP2016565064A patent/JP2017513510A/ja active Pending
- 2015-04-28 BR BR112016024945A patent/BR112016024945A2/pt active IP Right Grant
- 2015-04-28 AU AU2015253352A patent/AU2015253352A1/en not_active Abandoned
- 2015-04-28 CN CN201580030358.1A patent/CN106535630B/zh active Active
- 2015-04-28 EP EP15723593.8A patent/EP3136850A1/en not_active Withdrawn
- 2015-04-28 BR BR122023023211-9A patent/BR122023023211A2/pt unknown
- 2015-04-28 US US14/698,561 patent/US20160029604A1/en not_active Abandoned
- 2015-04-28 CN CN202010235249.6A patent/CN111647627A/zh active Pending
- 2015-04-28 KR KR1020167032557A patent/KR20170003585A/ko not_active Withdrawn
- 2015-04-28 CA CA2946881A patent/CA2946881A1/en not_active Abandoned
- 2015-04-28 MX MX2016014066A patent/MX2016014066A/es unknown
- 2015-04-28 WO PCT/US2015/027995 patent/WO2015168125A1/en not_active Ceased
- 2015-04-28 AR ARP150101276A patent/AR100216A1/es unknown
-
2016
- 2016-10-28 PH PH12016502162A patent/PH12016502162A1/en unknown
-
2018
- 2018-03-16 US US15/923,951 patent/US20180235194A1/en not_active Abandoned
-
2021
- 2021-07-19 US US17/379,898 patent/US12070022B2/en active Active
-
2024
- 2024-07-12 US US18/771,458 patent/US20240381856A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20180235194A1 (en) | 2018-08-23 |
| PH12016502162A1 (en) | 2017-02-06 |
| BR112016024945A2 (pt) | 2017-10-24 |
| US20240381856A1 (en) | 2024-11-21 |
| US20220056482A1 (en) | 2022-02-24 |
| MX2016014066A (es) | 2017-05-03 |
| AP2016009588A0 (en) | 2016-11-30 |
| JP2017513510A (ja) | 2017-06-01 |
| CN111647627A (zh) | 2020-09-11 |
| US12070022B2 (en) | 2024-08-27 |
| CA2946881A1 (en) | 2015-11-05 |
| BR122023023211A2 (pt) | 2024-01-23 |
| AU2015253352A1 (en) | 2016-12-08 |
| EP3136850A1 (en) | 2017-03-08 |
| CN106535630A (zh) | 2017-03-22 |
| KR20170003585A (ko) | 2017-01-09 |
| WO2015168125A1 (en) | 2015-11-05 |
| CN106535630B (zh) | 2020-04-24 |
| US20160029604A1 (en) | 2016-02-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AR100216A1 (es) | Edición de genes multiplexados | |
| EA201691581A1 (ru) | Способы и композиции для повышения эффективности направленной модификации генов с применением опосредованной олигонуклеотидами репарации генов | |
| MX2017008190A (es) | Metodos y composiciones para la modificacion genetica dirigida a traves de direccionamiento multiple de una sola etapa. | |
| EA201391373A1 (ru) | Способы получения сложного локуса трансгенных признаков | |
| MX2020004541A (es) | Edicion de genes de celulas primarias. | |
| BR112017007770A2 (pt) | cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc. | |
| AR101080A1 (es) | Transformación de maíz haploide con una nucleasa específica de sitio | |
| BR112017012765A2 (pt) | ?métodos para editar uma sequência de nucleotídeos, célula e linhagem de e. coli e método para produzir uma célula de e. coli? | |
| MX2018001617A (es) | Composiciones de crispr-cas9 diseñados y metodos de uso. | |
| EA201792663A1 (ru) | Способы скрининга бактерий, архей, водорослей и дрожжей с использованием нуклеиновых кислот crispr | |
| MX2016014129A (es) | Composiciones para el control de mosquitos y usos de las mismas. | |
| BR112017000621A2 (pt) | método para melhorar um traço agronômico de uma planta, método para melhorar a produtividade de grãos ou a eficiência de uso de nitrogênio de uma planta de milho ou de cultura, método para editar, substituir ou eliminar uma sequência reguladora, método para inserir um promotor ou elemento do promotor no genoma de uma célula vegetal e método para fornecer um perfil de expressão adicional para um polinucleotídeo endógeno de uma célula vegetal | |
| MX2017016688A (es) | Nuevas enzimas y sistemas crispr. | |
| BR112015023398A8 (pt) | Métodos para amplificar ácidos nucléicos | |
| MX2021014528A (es) | Metodos y composiciones para generar alelos dominantes usando edicion del genoma. | |
| SG10201902811TA (en) | High efficiency, high throughput generation of genetically modified mammals by electroporation | |
| BR112017012900A2 (pt) | polinucleotídeo isolado ou um dna recombinante, célula de planta, planta, explante, semente, método para produzir uma célula de planta, método para controlar ervas daninhas, célula hospedeira | |
| AR109941A1 (es) | Generación de maíz resistente al tizón de la hoja del norte | |
| UY37301A (es) | Metodologías y composiciones para crear una recombinación dirigida y romper el enlace entre rasgos | |
| CL2017002789A1 (es) | Composiciones y métodos para controlar infestasiones de plagas y parásitos de los artrópodos | |
| AR109700A1 (es) | Optimización dirigida de genomas en plantas | |
| MX390143B (es) | Secuencias recombinantes de cromosoma b de maíz y sus usos | |
| BR112016016772A2 (pt) | Método para obter células vegetais transformadas | |
| CL2021000371A1 (es) | Autoselección de artrópodos machos estériles. | |
| BR112017002448A2 (pt) | método de identificação de planta de milho com helmintosporiose e método de introgressão de alelos |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |