AR100216A1 - Edición de genes multiplexados - Google Patents
Edición de genes multiplexadosInfo
- Publication number
- AR100216A1 AR100216A1 ARP150101276A ARP150101276A AR100216A1 AR 100216 A1 AR100216 A1 AR 100216A1 AR P150101276 A ARP150101276 A AR P150101276A AR P150101276 A ARP150101276 A AR P150101276A AR 100216 A1 AR100216 A1 AR 100216A1
- Authority
- AR
- Argentina
- Prior art keywords
- cell
- interest
- embryo
- chromosomal dna
- site
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 239000013611 chromosomal DNA Substances 0.000 abstract 7
- 210000001161 mammalian embryo Anatomy 0.000 abstract 7
- 241000251539 Vertebrata <Metazoa> Species 0.000 abstract 6
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 abstract 6
- 230000002068 genetic effect Effects 0.000 abstract 4
- 108010042407 Endonucleases Proteins 0.000 abstract 2
- 102000004533 Endonucleases Human genes 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000005496 tempering Methods 0.000 abstract 2
- 238000010459 TALEN Methods 0.000 abstract 1
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 abstract 1
- 230000001448 gametogenic effect Effects 0.000 abstract 1
- 238000003209 gene knockout Methods 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 230000000920 spermatogeneic effect Effects 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/101—Bovine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/108—Swine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461985327P | 2014-04-28 | 2014-04-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR100216A1 true AR100216A1 (es) | 2016-09-21 |
Family
ID=53190022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP150101276A AR100216A1 (es) | 2014-04-28 | 2015-04-28 | Edición de genes multiplexados |
Country Status (13)
| Country | Link |
|---|---|
| US (4) | US20160029604A1 (enExample) |
| EP (1) | EP3136850A1 (enExample) |
| JP (1) | JP2017513510A (enExample) |
| KR (1) | KR20170003585A (enExample) |
| CN (2) | CN111647627A (enExample) |
| AP (1) | AP2016009588A0 (enExample) |
| AR (1) | AR100216A1 (enExample) |
| AU (1) | AU2015253352A1 (enExample) |
| BR (2) | BR112016024945A2 (enExample) |
| CA (1) | CA2946881A1 (enExample) |
| MX (1) | MX2016014066A (enExample) |
| PH (1) | PH12016502162A1 (enExample) |
| WO (1) | WO2015168125A1 (enExample) |
Families Citing this family (99)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10920242B2 (en) | 2011-02-25 | 2021-02-16 | Recombinetics, Inc. | Non-meiotic allele introgression |
| US9528124B2 (en) | 2013-08-27 | 2016-12-27 | Recombinetics, Inc. | Efficient non-meiotic allele introgression |
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| KR101566498B1 (ko) | 2013-11-13 | 2015-11-06 | 건국대학교 산학협력단 | 인터루킨 2 수용체 감마 유전자 적중벡터, 그 벡터가 도입된 면역세포 결핍 형질전환 미니 복제돼지 생산과 그 제조방법 및 활용 |
| US20150166984A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting alpha-antitrypsin point mutations |
| US20160029604A1 (en) * | 2014-04-28 | 2016-02-04 | Recombinetics, Inc. | Multiplex gene editing |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| AU2015320738B2 (en) | 2014-09-23 | 2021-12-02 | Acceligen, Inc. | Materials and methods for producing animals with short hair |
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
| CN108024522A (zh) * | 2015-03-03 | 2018-05-11 | 明尼苏达大学董事会 | Etv2及其用途 |
| MX2018000273A (es) * | 2015-06-30 | 2018-03-08 | Univ Minnesota | Musculo esqueletico humanizado. |
| BR112017028608A2 (pt) | 2015-06-30 | 2018-09-04 | Univ Minnesota | músculo cardíaco humanizado |
| EP3353296B1 (en) | 2015-09-24 | 2020-11-04 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| US20170099813A1 (en) * | 2015-10-07 | 2017-04-13 | Recombinetics, Inc. | Method of generating sterile terminal sires in livestock and animals produced thereby |
| SG10202104041PA (en) | 2015-10-23 | 2021-06-29 | Harvard College | Nucleobase editors and uses thereof |
| CN105567689B (zh) * | 2016-01-25 | 2019-04-09 | 重庆威斯腾生物医药科技有限责任公司 | CRISPR/Cas9靶向敲除人TCAB1基因及其特异性gRNA |
| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| WO2017180694A1 (en) | 2016-04-13 | 2017-10-19 | Editas Medicine, Inc. | Cas9 fusion molecules gene editing systems, and methods of use thereof |
| JP6974349B2 (ja) * | 2016-04-18 | 2021-12-01 | クリスパー セラピューティクス アクチェンゲゼルシャフト | ヘモグロビン異常症の処置のための材料及び方法 |
| CN106191061B (zh) * | 2016-07-18 | 2019-06-18 | 暨南大学 | 一种特异靶向人ABCG2基因的sgRNA导向序列及其应用 |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US20180084767A1 (en) | 2016-09-21 | 2018-03-29 | Recombinetics, Inc. | Animal models for cardiomyopathy |
| SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
| AU2017362513A1 (en) * | 2016-11-18 | 2019-05-16 | Christopher Bradley | Massively multiplexed homologous template repair for whole-genome replacement |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| AU2018221730B2 (en) | 2017-02-15 | 2024-06-20 | Novo Nordisk A/S | Donor repair templates multiplex genome editing |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| WO2018176009A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| CN106987604B (zh) * | 2017-03-29 | 2021-05-28 | 北京希诺谷生物科技有限公司 | 一种制备动脉粥样硬化疾病模型犬的方法 |
| AU2018243383B2 (en) * | 2017-03-30 | 2024-12-05 | Monsanto Technology Llc | Systems and methods for use in identifying multiple genome edits and predicting the aggregate effects of the identified genome edits |
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| WO2018197520A1 (en) * | 2017-04-24 | 2018-11-01 | Dupont Nutrition Biosciences Aps | Methods and compositions of anti-crispr proteins for use in plants |
| CN107974462A (zh) * | 2017-04-28 | 2018-05-01 | 中山大学 | 一种通过延迟基因的表达时间来提高猪瘦肉产量的方法 |
| CN107182940B (zh) | 2017-05-09 | 2020-05-15 | 中国科学院动物研究所 | 一种抗寒及瘦肉型转基因猪及其制备方法 |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| WO2019014564A1 (en) | 2017-07-14 | 2019-01-17 | Editas Medicine, Inc. | SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES |
| CN107937445B (zh) * | 2017-07-25 | 2021-05-04 | 北京希诺谷生物科技有限公司 | 利用体细胞克隆技术制备基因敲除犬的方法 |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
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| CN109321600A (zh) * | 2018-10-19 | 2019-02-12 | 中国农业大学 | 一种培育生产低致敏性牛奶的牛的方法及其应用 |
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| CN109735630A (zh) * | 2019-01-05 | 2019-05-10 | 兰州大学 | 绵羊zfy基因单核苷酸多态性标记的检测方法及标记应用 |
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| CN111484994B (zh) * | 2019-01-29 | 2022-04-19 | 四川大学华西医院 | CRISPR-Cas9特异性敲除猪Fah、Rag2双基因的方法 |
| CN109777834A (zh) * | 2019-02-25 | 2019-05-21 | 中国科学院广州生物医药与健康研究院 | 一种重度免疫缺陷猪模型及其构建方法和应用 |
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| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US11859213B2 (en) | 2019-05-16 | 2024-01-02 | Regents Of The University Of Minnesota | Development of superior chimerism by hiPSC engineering and embryo aggregation |
| CN110452929B (zh) * | 2019-07-09 | 2021-07-20 | 中山大学 | 一种非嵌合基因编辑猪胚胎模型的构建方法 |
| WO2021011561A1 (en) * | 2019-07-15 | 2021-01-21 | The General Hospital Corporation | Zebrafish deletion and compound mutants and uses thereof |
| CN110305872A (zh) * | 2019-07-17 | 2019-10-08 | 中国农业科学院北京畜牧兽医研究所 | 小型猪2型糖尿病模型的构建方法及应用 |
| US20220282284A1 (en) * | 2019-08-15 | 2022-09-08 | The Rockefeller University | Crispr genome editing with cell surface display to produce homozygously edited eukaryotic cells |
| CN112538497B (zh) * | 2019-09-20 | 2023-02-28 | 南京启真基因工程有限公司 | CRISPR/Cas9系统及其在构建α、β和α&β地中海贫血模型猪细胞系中的应用 |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021102084A1 (en) | 2019-11-22 | 2021-05-27 | President And Fellows Of Harvard College | Ionic liquids for drug delivery |
| KR102270145B1 (ko) * | 2019-12-27 | 2021-07-01 | 서울대학교산학협력단 | Btk 넉아웃 질환모델용 돼지 및 이의 용도 |
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| US20220056482A1 (en) | 2022-02-24 |
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