BR122023023211A2 - Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano - Google Patents

Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano Download PDF

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Publication number
BR122023023211A2
BR122023023211A2 BR122023023211-9A BR122023023211A BR122023023211A2 BR 122023023211 A2 BR122023023211 A2 BR 122023023211A2 BR 122023023211 A BR122023023211 A BR 122023023211A BR 122023023211 A2 BR122023023211 A2 BR 122023023211A2
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gene
cell
cells
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dna
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BR122023023211-9A
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Portuguese (pt)
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Scott C. Fahrenkrug
Daniel F. Carlson
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Recombinetics, Inc.
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BR122023023211-9A 2014-04-28 2015-04-28 Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano BR122023023211A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201461985327P 2014-04-28 2014-04-28
US61/985,327 2014-04-28
PCT/US2015/027995 WO2015168125A1 (en) 2014-04-28 2015-04-28 Multiplex gene editing in swine
BR112016024945A BR112016024945A2 (pt) 2014-04-28 2015-04-28 edição de gene multiplex em suínos

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BR122023023211A2 true BR122023023211A2 (pt) 2024-01-23

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BR122023023211-9A BR122023023211A2 (pt) 2014-04-28 2015-04-28 Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano

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US (4) US20160029604A1 (enExample)
EP (1) EP3136850A1 (enExample)
JP (1) JP2017513510A (enExample)
KR (1) KR20170003585A (enExample)
CN (2) CN106535630B (enExample)
AP (1) AP2016009588A0 (enExample)
AR (1) AR100216A1 (enExample)
AU (1) AU2015253352A1 (enExample)
BR (2) BR112016024945A2 (enExample)
CA (1) CA2946881A1 (enExample)
MX (1) MX2016014066A (enExample)
PH (1) PH12016502162A1 (enExample)
WO (1) WO2015168125A1 (enExample)

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
EP2734621B1 (en) 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
KR101566498B1 (ko) * 2013-11-13 2015-11-06 건국대학교 산학협력단 인터루킨 2 수용체 감마 유전자 적중벡터, 그 벡터가 도입된 면역세포 결핍 형질전환 미니 복제돼지 생산과 그 제조방법 및 활용
US9840699B2 (en) 2013-12-12 2017-12-12 President And Fellows Of Harvard College Methods for nucleic acid editing
BR112016024945A2 (pt) * 2014-04-28 2017-10-24 Recombinetics Inc edição de gene multiplex em suínos
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
BR112017005483A2 (pt) 2014-09-23 2018-03-06 Aggenetics Inc materiais e métodos para a produção de animais com pelo curto
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP4464338A3 (en) 2014-11-07 2025-02-12 Editas Medicine, Inc. Systems for improving crispr/cas-mediated genome-editing
WO2016141234A1 (en) * 2015-03-03 2016-09-09 Regents Of The University Of Minnesota Etv2 and uses thereof
BR112017028608A2 (pt) * 2015-06-30 2018-09-04 Univ Minnesota músculo cardíaco humanizado
CN108135943A (zh) * 2015-06-30 2018-06-08 明尼苏达大学校董会 人源化的骨骼肌
AU2016326711B2 (en) 2015-09-24 2022-11-03 Editas Medicine, Inc. Use of exonucleases to improve CRISPR/Cas-mediated genome editing
US20170099813A1 (en) * 2015-10-07 2017-04-13 Recombinetics, Inc. Method of generating sterile terminal sires in livestock and animals produced thereby
IL258821B (en) 2015-10-23 2022-07-01 Harvard College Nucleobase editors and their uses
CN105567689B (zh) * 2016-01-25 2019-04-09 重庆威斯腾生物医药科技有限责任公司 CRISPR/Cas9靶向敲除人TCAB1基因及其特异性gRNA
WO2017165826A1 (en) 2016-03-25 2017-09-28 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
EP4047092B1 (en) 2016-04-13 2025-07-30 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
MX2018012729A (es) * 2016-04-18 2019-07-04 Crispr Therapeutics Ag Materiales y metodos para el tratamiento de hemoglobinopatias.
CN106191061B (zh) * 2016-07-18 2019-06-18 暨南大学 一种特异靶向人ABCG2基因的sgRNA导向序列及其应用
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US20180084767A1 (en) 2016-09-21 2018-03-29 Recombinetics, Inc. Animal models for cardiomyopathy
JP7588390B2 (ja) 2016-10-14 2024-11-22 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸塩基エディターのａａｖ送達
EP3541937A4 (en) * 2016-11-18 2020-06-24 Christopher Bradley REPAIR OF MASSIVELY MULTIPLEXED HOMOLOGICAL TEMPLATE FOR FULL GENERATION
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
DK3583203T5 (da) 2017-02-15 2024-09-02 2Seventy Bio Inc Donorreparationstemplates multiplex-genomeditering
CN110662556A (zh) 2017-03-09 2020-01-07 哈佛大学的校长及成员们 癌症疫苗
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
BR112019019655A2 (pt) 2017-03-23 2020-04-22 Harvard College editores de nucleobase que compreendem proteínas de ligação a dna programáveis por ácido nucleico
CN106987604B (zh) * 2017-03-29 2021-05-28 北京希诺谷生物科技有限公司 一种制备动脉粥样硬化疾病模型犬的方法
EP3610398A4 (en) * 2017-03-30 2021-02-24 Monsanto Technology LLC SYSTEMS AND METHODS FOR USE IN THE IDENTIFICATION OF MULTIPLE GENOME EDITS AND IN THE AGGREGATE EFFECTS FORECAST OF THE IDENTIFIED GENOME EDITS
CN111031790A (zh) 2017-04-20 2020-04-17 E开创生物技术股份有限公司 产生基因修改的动物的方法
CN110891965A (zh) * 2017-04-24 2020-03-17 杜邦营养生物科学有限公司 植物中使用的抗crispr蛋白的方法和组合物
CN107974462A (zh) * 2017-04-28 2018-05-01 中山大学 一种通过延迟基因的表达时间来提高猪瘦肉产量的方法
CN107182940B (zh) 2017-05-09 2020-05-15 中国科学院动物研究所 一种抗寒及瘦肉型转基因猪及其制备方法
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
CN107937445B (zh) * 2017-07-25 2021-05-04 北京希诺谷生物科技有限公司 利用体细胞克隆技术制备基因敲除犬的方法
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
US20190045761A1 (en) 2017-08-11 2019-02-14 Recombinetics, Inc. Inducible disease models methods of making them and use in tissue complementation
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
KR102207352B1 (ko) * 2017-09-29 2021-01-26 서울대학교산학협력단 Klotho 유전자 넉아웃 질환모델 동물 및 이의 용도
US20210206814A1 (en) * 2017-10-12 2021-07-08 Mayo Foundation For Medical Education And Research Methods and materials for gene editing
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
MA51107A (fr) 2017-12-05 2020-10-14 Vertex Pharma Cellules souches et progénitrices hématopoïétiques humaines cd34+ modifiées par crispr-cas9 et utilisations associées
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
EP3749767A1 (en) 2018-02-05 2020-12-16 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of hemoglobinopathies
CN108504685A (zh) * 2018-03-27 2018-09-07 宜明细胞生物科技有限公司 一种利用CRISPR/Cas9系统同源重组修复IL-2RG缺陷基因的方法
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
KR102103103B1 (ko) 2018-08-16 2020-04-21 (주)라트바이오 인위적 뉴클레아제를 생산하는 형질전환 동물 및 형질전환 배아
CN110846330B (zh) * 2018-08-21 2021-08-27 中国科学院动物研究所 一种突变基因及其用于构建蹼足病小型猪模型的用途
CN109321600A (zh) * 2018-10-19 2019-02-12 中国农业大学 一种培育生产低致敏性牛奶的牛的方法及其应用
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
CN109735630A (zh) * 2019-01-05 2019-05-10 兰州大学 绵羊zfy基因单核苷酸多态性标记的检测方法及标记应用
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
CN111484994B (zh) * 2019-01-29 2022-04-19 四川大学华西医院 CRISPR-Cas9特异性敲除猪Fah、Rag2双基因的方法
CN109777834A (zh) * 2019-02-25 2019-05-21 中国科学院广州生物医药与健康研究院 一种重度免疫缺陷猪模型及其构建方法和应用
EP3942042A1 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
EP3956349A1 (en) 2019-04-17 2022-02-23 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US11859213B2 (en) 2019-05-16 2024-01-02 Regents Of The University Of Minnesota Development of superior chimerism by hiPSC engineering and embryo aggregation
CN110452929B (zh) * 2019-07-09 2021-07-20 中山大学 一种非嵌合基因编辑猪胚胎模型的构建方法
WO2021011561A1 (en) * 2019-07-15 2021-01-21 The General Hospital Corporation Zebrafish deletion and compound mutants and uses thereof
CN110305872A (zh) * 2019-07-17 2019-10-08 中国农业科学院北京畜牧兽医研究所 小型猪2型糖尿病模型的构建方法及应用
WO2021030735A1 (en) * 2019-08-15 2021-02-18 The Rockefeller University Crispr genome editing with cell surface display to produce homozygously edited eukaryotic cells
CN112538497B (zh) * 2019-09-20 2023-02-28 南京启真基因工程有限公司 CRISPR/Cas9系统及其在构建α、β和α＆β地中海贫血模型猪细胞系中的应用
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
IL293145A (en) 2019-11-22 2022-07-01 Harvard College Ionic liquids for drug delivery
KR102270145B1 (ko) * 2019-12-27 2021-07-01 서울대학교산학협력단 Btk 넉아웃 질환모델용 돼지 및 이의 용도
WO2021150712A1 (en) * 2020-01-21 2021-07-29 The Board Of Regents Of The University Of Texas System Modulating tlr/nf-kb and p53 signaling pathways to enhance interspecies chimerism between evolutionary distant species
WO2021226558A1 (en) 2020-05-08 2021-11-11 The Broad Institute, Inc. Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
CN111334529B (zh) * 2020-05-20 2020-09-29 中国农业大学 一种利用第三代碱基编辑器制备精准blg基因敲除牛的方法
CN111534519B (zh) * 2020-05-26 2021-12-14 中国农业科学院北京畜牧兽医研究所 识别猪eIF4G1基因的sgRNA及其编码DNA和应用
CA3180217A1 (en) * 2020-06-02 2021-12-09 Gregory T. Bleck Nucleic acid constructs for protein manufacture
CN113234722B (zh) * 2021-04-30 2023-03-14 肇庆市瑞思元生物科技有限公司 利用碱基编辑修复与板层状鱼鳞病相关的tgm1 c607t突变的试剂和方法
CN113583944B (zh) * 2021-09-15 2023-07-21 广西壮族自治区水牛研究所 激活Wnt/β-catenin信号通路在提高水牛体外胚胎生产效率中的应用
IL311885A (en) 2021-10-08 2024-06-01 Harvard College Ionic liquids for drug delivery
WO2023077360A1 (zh) * 2021-11-04 2023-05-11 云南农业大学 一种构建重症免疫缺陷和肝损伤双重猪模型的方法及应用
US20250300846A1 (en) * 2021-12-17 2025-09-25 Laurie Schmelzle Systems and methods for generating, managing, and displaying non-fungible digital representations of living, biological, and/or eukaryotic organisms
CN115341044A (zh) * 2022-10-19 2022-11-15 佛山科学技术学院 一种利用微生物及其相关snp位点预测猪日增重的方法
CN115786343B (zh) * 2022-11-17 2024-08-30 石河子大学 一种猪Zfy基因的RNA干扰片段、表达载体及其应用
CN116334087A (zh) * 2023-05-10 2023-06-27 山东农业大学 特异性靶向猪Pax3基因的sgRNA序列及其应用
CN116790604B (zh) * 2023-08-18 2023-10-27 成都中科奥格生物科技有限公司 一种sgRNA、CRISPR/Cas9载体及其构建方法和用途
CN118910057B (zh) * 2024-09-04 2025-03-21 江苏农牧科技职业学院 用于敲除猪RBM20基因部分保守序列的sgRNA组合、猪模型和构建方法
CN119432858A (zh) * 2025-01-13 2025-02-14 江苏省农业科学院 一种用于精准编辑奶牛BLG基因的sgRNA组合、引物及应用
CN120204361A (zh) * 2025-03-28 2025-06-27 中国科学院水生生物研究所 Hnf4α或含Hnf4α的生物材料在制备抑制细菌和/或病毒产品中的应用

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
EP0821070A1 (en) 1996-07-22 1998-01-28 Carelli, Claude Marcel Henri Pit-1 gene polymorphism and trait selection in animals
WO2000022098A1 (en) 1998-10-12 2000-04-20 Geron Bio-Med Limited Porcine oocytes with improved developmental competence
WO2001030965A2 (en) 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
EP1476547B1 (en) 2002-01-23 2006-12-06 The University of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
CN101405404A (zh) 2002-12-31 2009-04-08 迈特默菲公司 用于推断牛的性状的组合物、方法和系统
US7985739B2 (en) 2003-06-04 2011-07-26 The Board Of Trustees Of The Leland Stanford Junior University Enhanced sleeping beauty transposon system and methods for using the same
US20050153317A1 (en) 2003-10-24 2005-07-14 Metamorphix, Inc. Methods and systems for inferring traits to breed and manage non-beef livestock
CA2563064A1 (en) * 2004-04-22 2005-11-10 Kirin Beer Kabushiki Kaisha Transgenic animals and uses thereof
US20060130157A1 (en) * 2004-10-22 2006-06-15 Kevin Wells Ungulates with genetically modified immune systems
ES2459640T3 (es) 2005-06-24 2014-05-12 Recombinetics, Inc. Uso de citosina desaminasas para disminuir la transferencia de retroelementos desde cerdos a seres humanos
AU2008305590A1 (en) * 2007-09-27 2009-04-02 Sangamo Therapeutics, Inc. Genomic editing in zebrafish using zinc finger nucleases
WO2010008562A2 (en) 2008-07-16 2010-01-21 Recombinetics Methods and materials for producing transgenic animals
US20110030072A1 (en) * 2008-12-04 2011-02-03 Sigma-Aldrich Co. Genome editing of immunodeficiency genes in animals
US9206404B2 (en) * 2008-12-04 2015-12-08 Sangamo Biosciences, Inc. Method of deleting an IgM gene in an isolated rat cell
US20110023159A1 (en) 2008-12-04 2011-01-27 Sigma-Aldrich Co. Ovine genome editing with zinc finger nucleases
US20110023140A1 (en) 2008-12-04 2011-01-27 Sigma-Aldrich Co. Rabbit genome editing with zinc finger nucleases
KR20100080068A (ko) 2008-12-31 2010-07-08 주식회사 툴젠 신규한 징크 핑거 뉴클레아제 및 이의 용도
CN102858985A (zh) * 2009-07-24 2013-01-02 西格马-奥尔德里奇有限责任公司 基因组编辑方法
US20120192298A1 (en) 2009-07-24 2012-07-26 Sigma Aldrich Co. Llc Method for genome editing
ES2550202T3 (es) 2009-08-03 2015-11-05 Recombinetics, Inc. Métodos y composiciones para la modificación dirigida de genes
WO2011020005A1 (en) 2009-08-14 2011-02-17 Regeneron Pharmaceuticals, Inc. miRNA-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE
NO2510096T3 (enExample) 2009-12-10 2015-03-21
WO2011100058A1 (en) * 2010-02-09 2011-08-18 Sangamo Biosciences, Inc. Targeted genomic modification with partially single-stranded donor molecules
BR112012020257A8 (pt) 2010-02-11 2018-02-14 Recombinetics Inc métodos e aparelhos para produzir artiodátilos transgênicos
JP5841322B2 (ja) 2010-04-22 2016-01-13 オレゴン ヘルス アンド サイエンス ユニバーシティ フマリルアセト酢酸ヒドロラーゼ（ｆａｈ）欠損性ブタおよびその使用
AU2011243085A1 (en) 2010-04-23 2012-10-11 E. I. Du Pont De Nemours And Company Gene switch compositions and methods of use
EP2392208B1 (en) 2010-06-07 2016-05-04 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Fusion proteins comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease and their use
CA2802591A1 (en) * 2010-06-17 2011-12-22 Kymab Limited Animal models and therapeutic molecules
KR20180121665A (ko) 2010-07-23 2018-11-07 시그마-알드리치 컴퍼니., 엘엘씨 표적화 엔도뉴클레아제 및 단일-가닥 핵산을 사용하는 게놈 편집
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
CA2828239C (en) * 2011-02-25 2020-10-06 Recombinetics, Inc. Genetically modified animals and methods for making the same
JP6158170B2 (ja) * 2011-04-27 2017-07-12 アミリス， インコーポレイテッド ゲノム修飾のための方法
BR112014020089A2 (pt) 2012-02-15 2017-07-04 Mice With Horns Llc animais transgênicos com características que podem ser customizadas
SI3401400T1 (sl) 2012-05-25 2019-10-30 Univ California Postopki in sestavki za RNA usmerjeno modifikacijo tarčne DNA in za RNA usmerjeno modulacijo prepisovanja
AR091482A1 (es) * 2012-06-21 2015-02-04 Recombinetics Inc Celulas modificadas geneticamente y metodos par su obtencion
US10058078B2 (en) 2012-07-31 2018-08-28 Recombinetics, Inc. Production of FMDV-resistant livestock by allele substitution
US20140115728A1 (en) 2012-10-24 2014-04-24 A. Joseph Tector Double knockout (gt/cmah-ko) pigs, organs and tissues
EP2914714A4 (en) 2012-10-30 2016-09-21 Recombinetics Inc CONTROLLING GENDER TREATMENT IN ANIMALS
IL311744B2 (en) 2012-11-05 2025-10-01 Regeneron Pharma Genetically modified non-human animals and methods of using them
EP3138911B1 (en) 2012-12-06 2018-12-05 Sigma Aldrich Co. LLC Crispr-based genome modification and regulation
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
CN105658796B (zh) 2012-12-12 2021-10-26 布罗德研究所有限公司 用于序列操纵的crispr-cas组分系统、方法以及组合物
DK3553174T3 (da) 2012-12-17 2025-08-04 Harvard College Rna-guided modificering af humant genom
US10612043B2 (en) 2013-03-09 2020-04-07 Agilent Technologies, Inc. Methods of in vivo engineering of large sequences using multiple CRISPR/cas selections of recombineering events
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
CN105121650A (zh) 2013-04-02 2015-12-02 拜尔作物科学公司 真核生物中的靶向基因组工程
WO2014189680A1 (en) 2013-05-19 2014-11-27 Mice With Horns, Llc Transgenic animals with customizable traits
US20140359795A1 (en) 2013-05-31 2014-12-04 Recombinetics, Inc. Genetic techniques for making animals with sortable sperm
US20140359796A1 (en) 2013-05-31 2014-12-04 Recombinetics, Inc. Genetically sterile animals
KR20230136697A (ko) 2013-06-05 2023-09-26 듀크 유니버시티 Rna-가이드 유전자 편집 및 유전자 조절
RU2764637C2 (ru) 2013-07-09 2022-01-19 Президент Энд Фэллоуз Оф Харвард Коллидж Мультиплексная геномная инженерия, направляемая рнк
US9670458B2 (en) 2013-07-13 2017-06-06 Aggenetics, Inc. Methods for spermatogonial stem cell (SSC) transfer
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US20150064149A1 (en) 2013-09-04 2015-03-05 Mice With Horns, LLC. Materials and methods for correcting recessive mutations in animals
WO2015052231A2 (en) 2013-10-08 2015-04-16 Technical University Of Denmark Multiplex editing system
US10787684B2 (en) 2013-11-19 2020-09-29 President And Fellows Of Harvard College Large gene excision and insertion
PT3105328T (pt) 2014-02-11 2020-07-06 Univ Colorado Regents Engenharia de genomas multiplexada possibilitada por crispr
BR112016024945A2 (pt) 2014-04-28 2017-10-24 Recombinetics Inc edição de gene multiplex em suínos

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