KR20170003585A - 돼지에서 멀티플렉스 유전자 편집 - Google Patents

돼지에서 멀티플렉스 유전자 편집 Download PDF

Info

Publication number
KR20170003585A
KR20170003585A KR1020167032557A KR20167032557A KR20170003585A KR 20170003585 A KR20170003585 A KR 20170003585A KR 1020167032557 A KR1020167032557 A KR 1020167032557A KR 20167032557 A KR20167032557 A KR 20167032557A KR 20170003585 A KR20170003585 A KR 20170003585A
Authority
KR
South Korea
Prior art keywords
gene
cell
cells
animal
embryo
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
KR1020167032557A
Other languages
English (en)
Korean (ko)
Inventor
스캇 씨. 파렌크루그
다니엘 에프. 칼슨
Original Assignee
리컴비네틱스 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 리컴비네틱스 인코포레이티드 filed Critical 리컴비네틱스 인코포레이티드
Publication of KR20170003585A publication Critical patent/KR20170003585A/ko
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020167032557A 2014-04-28 2015-04-28 돼지에서 멀티플렉스 유전자 편집 Withdrawn KR20170003585A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461985327P 2014-04-28 2014-04-28
US61/985,327 2014-04-28
PCT/US2015/027995 WO2015168125A1 (en) 2014-04-28 2015-04-28 Multiplex gene editing in swine

Publications (1)

Publication Number Publication Date
KR20170003585A true KR20170003585A (ko) 2017-01-09

Family

ID=53190022

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020167032557A Withdrawn KR20170003585A (ko) 2014-04-28 2015-04-28 돼지에서 멀티플렉스 유전자 편집

Country Status (13)

Country Link
US (4) US20160029604A1 (enExample)
EP (1) EP3136850A1 (enExample)
JP (1) JP2017513510A (enExample)
KR (1) KR20170003585A (enExample)
CN (2) CN111647627A (enExample)
AP (1) AP2016009588A0 (enExample)
AR (1) AR100216A1 (enExample)
AU (1) AU2015253352A1 (enExample)
BR (2) BR112016024945A2 (enExample)
CA (1) CA2946881A1 (enExample)
MX (1) MX2016014066A (enExample)
PH (1) PH12016502162A1 (enExample)
WO (1) WO2015168125A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102270145B1 (ko) * 2019-12-27 2021-07-01 서울대학교산학협력단 Btk 넉아웃 질환모델용 돼지 및 이의 용도

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
KR101566498B1 (ko) 2013-11-13 2015-11-06 건국대학교 산학협력단 인터루킨 2 수용체 감마 유전자 적중벡터, 그 벡터가 도입된 면역세포 결핍 형질전환 미니 복제돼지 생산과 그 제조방법 및 활용
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
US20160029604A1 (en) * 2014-04-28 2016-02-04 Recombinetics, Inc. Multiplex gene editing
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
AU2015320738B2 (en) 2014-09-23 2021-12-02 Acceligen, Inc. Materials and methods for producing animals with short hair
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2016073990A2 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
CN108024522A (zh) * 2015-03-03 2018-05-11 明尼苏达大学董事会 Etv2及其用途
MX2018000273A (es) * 2015-06-30 2018-03-08 Univ Minnesota Musculo esqueletico humanizado.
BR112017028608A2 (pt) 2015-06-30 2018-09-04 Univ Minnesota músculo cardíaco humanizado
EP3353296B1 (en) 2015-09-24 2020-11-04 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
US20170099813A1 (en) * 2015-10-07 2017-04-13 Recombinetics, Inc. Method of generating sterile terminal sires in livestock and animals produced thereby
SG10202104041PA (en) 2015-10-23 2021-06-29 Harvard College Nucleobase editors and uses thereof
CN105567689B (zh) * 2016-01-25 2019-04-09 重庆威斯腾生物医药科技有限责任公司 CRISPR/Cas9靶向敲除人TCAB1基因及其特异性gRNA
US11597924B2 (en) 2016-03-25 2023-03-07 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
WO2017180694A1 (en) 2016-04-13 2017-10-19 Editas Medicine, Inc. Cas9 fusion molecules gene editing systems, and methods of use thereof
JP6974349B2 (ja) * 2016-04-18 2021-12-01 クリスパー セラピューティクス アクチェンゲゼルシャフト ヘモグロビン異常症の処置のための材料及び方法
CN106191061B (zh) * 2016-07-18 2019-06-18 暨南大学 一种特异靶向人ABCG2基因的sgRNA导向序列及其应用
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US20180084767A1 (en) 2016-09-21 2018-03-29 Recombinetics, Inc. Animal models for cardiomyopathy
SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
AU2017362513A1 (en) * 2016-11-18 2019-05-16 Christopher Bradley Massively multiplexed homologous template repair for whole-genome replacement
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
AU2018221730B2 (en) 2017-02-15 2024-06-20 Novo Nordisk A/S Donor repair templates multiplex genome editing
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
WO2018176009A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
CN106987604B (zh) * 2017-03-29 2021-05-28 北京希诺谷生物科技有限公司 一种制备动脉粥样硬化疾病模型犬的方法
AU2018243383B2 (en) * 2017-03-30 2024-12-05 Monsanto Technology Llc Systems and methods for use in identifying multiple genome edits and predicting the aggregate effects of the identified genome edits
EP3612023A4 (en) 2017-04-20 2021-05-12 Egenesis, Inc. METHOD FOR PRODUCING GENETICALLY MODIFIED ANIMALS
WO2018197520A1 (en) * 2017-04-24 2018-11-01 Dupont Nutrition Biosciences Aps Methods and compositions of anti-crispr proteins for use in plants
CN107974462A (zh) * 2017-04-28 2018-05-01 中山大学 一种通过延迟基因的表达时间来提高猪瘦肉产量的方法
CN107182940B (zh) 2017-05-09 2020-05-15 中国科学院动物研究所 一种抗寒及瘦肉型转基因猪及其制备方法
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
WO2019014564A1 (en) 2017-07-14 2019-01-17 Editas Medicine, Inc. SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES
CN107937445B (zh) * 2017-07-25 2021-05-04 北京希诺谷生物科技有限公司 利用体细胞克隆技术制备基因敲除犬的方法
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
US20190045761A1 (en) 2017-08-11 2019-02-14 Recombinetics, Inc. Inducible disease models methods of making them and use in tissue complementation
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
KR102207352B1 (ko) * 2017-09-29 2021-01-26 서울대학교산학협력단 Klotho 유전자 넉아웃 질환모델 동물 및 이의 용도
WO2019075373A1 (en) * 2017-10-12 2019-04-18 Mayo Foundation For Medical Education And Research METHODS AND COMPOSITIONS FOR GENE EDITION
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
SG11202005147WA (en) 2017-12-05 2020-06-29 Vertex Pharma Crispr-cas9 modified cd34+ human hematopoietic stem and progenitor cells and uses thereof
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
WO2019150196A1 (en) 2018-02-05 2019-08-08 Crispr Therapeutics Ag Materials and methods for treatment of hemoglobinopathies
CN108504685A (zh) * 2018-03-27 2018-09-07 宜明细胞生物科技有限公司 一种利用CRISPR/Cas9系统同源重组修复IL-2RG缺陷基因的方法
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
KR102103103B1 (ko) 2018-08-16 2020-04-21 (주)라트바이오 인위적 뉴클레아제를 생산하는 형질전환 동물 및 형질전환 배아
CN110846330B (zh) * 2018-08-21 2021-08-27 中国科学院动物研究所 一种突变基因及其用于构建蹼足病小型猪模型的用途
CN109321600A (zh) * 2018-10-19 2019-02-12 中国农业大学 一种培育生产低致敏性牛奶的牛的方法及其应用
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
CN109735630A (zh) * 2019-01-05 2019-05-10 兰州大学 绵羊zfy基因单核苷酸多态性标记的检测方法及标记应用
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
CN111484994B (zh) * 2019-01-29 2022-04-19 四川大学华西医院 CRISPR-Cas9特异性敲除猪Fah、Rag2双基因的方法
CN109777834A (zh) * 2019-02-25 2019-05-21 中国科学院广州生物医药与健康研究院 一种重度免疫缺陷猪模型及其构建方法和应用
EP3942043A2 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US11859213B2 (en) 2019-05-16 2024-01-02 Regents Of The University Of Minnesota Development of superior chimerism by hiPSC engineering and embryo aggregation
CN110452929B (zh) * 2019-07-09 2021-07-20 中山大学 一种非嵌合基因编辑猪胚胎模型的构建方法
WO2021011561A1 (en) * 2019-07-15 2021-01-21 The General Hospital Corporation Zebrafish deletion and compound mutants and uses thereof
CN110305872A (zh) * 2019-07-17 2019-10-08 中国农业科学院北京畜牧兽医研究所 小型猪2型糖尿病模型的构建方法及应用
US20220282284A1 (en) * 2019-08-15 2022-09-08 The Rockefeller University Crispr genome editing with cell surface display to produce homozygously edited eukaryotic cells
CN112538497B (zh) * 2019-09-20 2023-02-28 南京启真基因工程有限公司 CRISPR/Cas9系统及其在构建α、β和α＆β地中海贫血模型猪细胞系中的应用
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021102084A1 (en) 2019-11-22 2021-05-27 President And Fellows Of Harvard College Ionic liquids for drug delivery
WO2021150712A1 (en) * 2020-01-21 2021-07-29 The Board Of Regents Of The University Of Texas System Modulating tlr/nf-kb and p53 signaling pathways to enhance interspecies chimerism between evolutionary distant species
BR112022022603A2 (pt) 2020-05-08 2023-01-17 Broad Inst Inc Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla
CN111334529B (zh) * 2020-05-20 2020-09-29 中国农业大学 一种利用第三代碱基编辑器制备精准blg基因敲除牛的方法
CN111534519B (zh) * 2020-05-26 2021-12-14 中国农业科学院北京畜牧兽医研究所 识别猪eIF4G1基因的sgRNA及其编码DNA和应用
BR112022024644A2 (pt) * 2020-06-02 2023-02-23 Catalent Pharma Solutions Llc Linhagens celulares com múltiplas ancoragens para inserção de gene
EP4212620A4 (en) * 2020-09-10 2024-10-23 JSR Corporation METHOD FOR PRODUCING OLIGODENDROCYTE-LIKE CELLS
CN113234722B (zh) * 2021-04-30 2023-03-14 肇庆市瑞思元生物科技有限公司 利用碱基编辑修复与板层状鱼鳞病相关的tgm1 c607t突变的试剂和方法
CN113583944B (zh) * 2021-09-15 2023-07-21 广西壮族自治区水牛研究所 激活Wnt/β-catenin信号通路在提高水牛体外胚胎生产效率中的应用
IL311885A (en) 2021-10-08 2024-06-01 Harvard College Ionic liquids for drug delivery
WO2023077360A1 (zh) * 2021-11-04 2023-05-11 云南农业大学 一种构建重症免疫缺陷和肝损伤双重猪模型的方法及应用
US20250300846A1 (en) * 2021-12-17 2025-09-25 Laurie Schmelzle Systems and methods for generating, managing, and displaying non-fungible digital representations of living, biological, and/or eukaryotic organisms
JP2023131904A (ja) * 2022-03-10 2023-09-22 国立大学法人愛媛大学 細胞の移植方法
CN115976018B (zh) * 2022-08-26 2025-12-30 南京启真基因工程有限公司 构建abca12基因突变的斑色鱼鳞癣模型猪核移植供体细胞的试剂盒及其应用
CN115341044A (zh) * 2022-10-19 2022-11-15 佛山科学技术学院 一种利用微生物及其相关snp位点预测猪日增重的方法
CN115786343B (zh) * 2022-11-17 2024-08-30 石河子大学 一种猪Zfy基因的RNA干扰片段、表达载体及其应用
CN116334087A (zh) * 2023-05-10 2023-06-27 山东农业大学 特异性靶向猪Pax3基因的sgRNA序列及其应用
CN116790604B (zh) * 2023-08-18 2023-10-27 成都中科奥格生物科技有限公司 一种sgRNA、CRISPR/Cas9载体及其构建方法和用途
CN117982624B (zh) * 2024-02-04 2026-01-27 中国科学院水生生物研究所 肝细胞核因子HNF4β在制备预防和/或治疗鱼类疾病渔用生物制品中的应用
CN118910057B (zh) * 2024-09-04 2025-03-21 江苏农牧科技职业学院 用于敲除猪RBM20基因部分保守序列的sgRNA组合、猪模型和构建方法
CN119432858A (zh) * 2025-01-13 2025-02-14 江苏省农业科学院 一种用于精准编辑奶牛BLG基因的sgRNA组合、引物及应用
CN120204361B (zh) * 2025-03-28 2026-02-03 中国科学院水生生物研究所 Hnf4α或含Hnf4α的生物材料在制备抑制细菌和/或病毒产品中的应用

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
EP0821070A1 (en) 1996-07-22 1998-01-28 Carelli, Claude Marcel Henri Pit-1 gene polymorphism and trait selection in animals
WO2000022098A1 (en) 1998-10-12 2000-04-20 Geron Bio-Med Limited Porcine oocytes with improved developmental competence
WO2001030965A2 (en) 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
AU2003251286B2 (en) 2002-01-23 2007-08-16 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
EP1590471A4 (en) 2002-12-31 2009-12-09 Metamorphix Inc COMPOSITIONS, METHODS AND SYSTEMS OF INFERENCE RELATING TO CATTLE CHARACTERISTICS
US7985739B2 (en) 2003-06-04 2011-07-26 The Board Of Trustees Of The Leland Stanford Junior University Enhanced sleeping beauty transposon system and methods for using the same
BRPI0415468A (pt) 2003-10-24 2007-03-27 Mmi Genomics Inc métodos e sistemas para inferir caracterìsticas para controlar criação não de gado
CA2563064A1 (en) * 2004-04-22 2005-11-10 Kirin Beer Kabushiki Kaisha Transgenic animals and uses thereof
NZ554824A (en) * 2004-10-22 2010-10-29 Revivicor Inc Porcines that lack endogenous antibody chains and express exogenous immunoglobulins
KR20080025172A (ko) 2005-06-24 2008-03-19 리전츠 오브 더 유니버스티 오브 미네소타 돼지에서 인간으로의 레트로엘리먼트 전달을 감소시키기위한 사이토신 탈아미노효소의 사용
US20090203140A1 (en) * 2007-09-27 2009-08-13 Sangamo Biosciences, Inc. Genomic editing in zebrafish using zinc finger nucleases
US8309791B2 (en) 2008-07-16 2012-11-13 Recombinectics, Inc. Method for producing a transgenic pig using a hyper-methylated transposon
US20110030072A1 (en) * 2008-12-04 2011-02-03 Sigma-Aldrich Co. Genome editing of immunodeficiency genes in animals
AU2009322964B2 (en) * 2008-12-04 2014-10-09 Sangamo Therapeutics, Inc. Genome editing in rats using zinc-finger nucleases
US20110023140A1 (en) 2008-12-04 2011-01-27 Sigma-Aldrich Co. Rabbit genome editing with zinc finger nucleases
US20110023159A1 (en) 2008-12-04 2011-01-27 Sigma-Aldrich Co. Ovine genome editing with zinc finger nucleases
KR20100080068A (ko) 2008-12-31 2010-07-08 주식회사 툴젠 신규한 징크 핑거 뉴클레아제 및 이의 용도
CN102858985A (zh) * 2009-07-24 2013-01-02 西格马-奥尔德里奇有限责任公司 基因组编辑方法
US20120192298A1 (en) 2009-07-24 2012-07-26 Sigma Aldrich Co. Llc Method for genome editing
ES2550202T3 (es) 2009-08-03 2015-11-05 Recombinetics, Inc. Métodos y composiciones para la modificación dirigida de genes
US8354389B2 (en) 2009-08-14 2013-01-15 Regeneron Pharmaceuticals, Inc. miRNA-regulated differentiation-dependent self-deleting cassette
NO2510096T3 (enExample) 2009-12-10 2015-03-21
JP2013518602A (ja) * 2010-02-09 2013-05-23 サンガモ バイオサイエンシーズ， インコーポレイテッド 部分的に一本鎖のドナー分子による標的化ゲノム改変
BR112012020257A8 (pt) 2010-02-11 2018-02-14 Recombinetics Inc métodos e aparelhos para produzir artiodátilos transgênicos
JP5841322B2 (ja) 2010-04-22 2016-01-13 オレゴン ヘルス アンド サイエンス ユニバーシティ フマリルアセト酢酸ヒドロラーゼ（ｆａｈ）欠損性ブタおよびその使用
US20110287936A1 (en) * 2010-04-23 2011-11-24 E.I. Dupont De Nemours And Company Gene switch compositions and methods of use
EP2392208B1 (en) 2010-06-07 2016-05-04 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Fusion proteins comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease and their use
CN103228130B (zh) * 2010-06-17 2016-03-16 科马布有限公司 动物模型及治疗分子
BR112013001685B1 (pt) * 2010-07-23 2021-10-13 Sigma-Aldrich Co. Llc Método para editar pelo menos uma sequência cromossômica endógena em uma célula pela inserção de uma sequência na sequência cromossômica
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
BR112013021785A8 (pt) 2011-02-25 2018-07-03 Recombinetics Inc animais geneticamente modificados e métodos para fazer os mesmos
WO2012149470A1 (en) * 2011-04-27 2012-11-01 Amyris, Inc. Methods for genomic modification
JP6282600B2 (ja) 2012-02-15 2018-02-21 エージージェネティクス インコーポレイテッド カスタマイズ可能な形質を有するトランスジェニック動物
LT3401400T (lt) 2012-05-25 2019-06-10 The Regents Of The University Of California Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui
AR091482A1 (es) * 2012-06-21 2015-02-04 Recombinetics Inc Celulas modificadas geneticamente y metodos par su obtencion
US10058078B2 (en) 2012-07-31 2018-08-28 Recombinetics, Inc. Production of FMDV-resistant livestock by allele substitution
US20140115728A1 (en) 2012-10-24 2014-04-24 A. Joseph Tector Double knockout (gt/cmah-ko) pigs, organs and tissues
AP2015008495A0 (en) 2012-10-30 2015-05-31 Ct For Aquaculture Technologies Inc Control of sexual maturation in animals
ES2884598T3 (es) 2012-11-05 2021-12-10 Regeneron Pharma Animales no humanos modificados genéticamente y métodos de uso de los mismos
EP3135765A1 (en) 2012-12-06 2017-03-01 Sigma-Aldrich Co. LLC Crispr-based genome modification and regulation
CN114634950A (zh) 2012-12-12 2022-06-17 布罗德研究所有限公司 用于序列操纵的crispr-cas组分系统、方法以及组合物
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
SG10201912991WA (en) 2012-12-17 2020-03-30 Harvard College Rna-guided human genome engineering
US10612043B2 (en) 2013-03-09 2020-04-07 Agilent Technologies, Inc. Methods of in vivo engineering of large sequences using multiple CRISPR/cas selections of recombineering events
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
AU2014247151A1 (en) 2013-04-02 2015-10-08 Bayer Cropscience Nv Targeted genome engineering in eukaryotes
WO2014189680A1 (en) 2013-05-19 2014-11-27 Mice With Horns, Llc Transgenic animals with customizable traits
US20140359795A1 (en) 2013-05-31 2014-12-04 Recombinetics, Inc. Genetic techniques for making animals with sortable sperm
US20140359796A1 (en) 2013-05-31 2014-12-04 Recombinetics, Inc. Genetically sterile animals
ES3029138T3 (en) 2013-06-05 2025-06-23 Univ Duke Rna-guided gene editing and gene regulation
KR20260047646A (ko) 2013-07-09 2026-04-08 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 멀티플렉스 rna-가이드된 게놈 조작
WO2015009571A1 (en) 2013-07-13 2015-01-22 Mice With Horns, Llc Improved methods for spermatogonial stem cell (ssc) transfer
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
WO2015035034A1 (en) 2013-09-04 2015-03-12 Mice With Horns, Llc Materials and methods for correcting recessive mutations in animals
WO2015052231A2 (en) 2013-10-08 2015-04-16 Technical University Of Denmark Multiplex editing system
US10787684B2 (en) 2013-11-19 2020-09-29 President And Fellows Of Harvard College Large gene excision and insertion
EP4063503A1 (en) 2014-02-11 2022-09-28 The Regents of the University of Colorado, a body corporate Crispr enabled multiplexed genome engineering
US20160029604A1 (en) * 2014-04-28 2016-02-04 Recombinetics, Inc. Multiplex gene editing

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102270145B1 (ko) * 2019-12-27 2021-07-01 서울대학교산학협력단 Btk 넉아웃 질환모델용 돼지 및 이의 용도

Also Published As

Publication number Publication date
JP2017513510A (ja) 2017-06-01
CA2946881A1 (en) 2015-11-05
BR122023023211A2 (pt) 2024-01-23
EP3136850A1 (en) 2017-03-08
US20240381856A1 (en) 2024-11-21
AR100216A1 (es) 2016-09-21
MX2016014066A (es) 2017-05-03
AU2015253352A1 (en) 2016-12-08
CN111647627A (zh) 2020-09-11
CN106535630B (zh) 2020-04-24
CN106535630A (zh) 2017-03-22
US12070022B2 (en) 2024-08-27
US20160029604A1 (en) 2016-02-04
AP2016009588A0 (en) 2016-11-30
PH12016502162A1 (en) 2017-02-06
BR112016024945A2 (pt) 2017-10-24
US20180235194A1 (en) 2018-08-23
US20220056482A1 (en) 2022-02-24
WO2015168125A1 (en) 2015-11-05

Similar Documents

Publication Publication Date Title
US12070022B2 (en) Methods for making genetic edits
US20200017882A1 (en) Engineering of humanized car t-cell and platelets by genetic complementation
US20190335725A1 (en) Genetically sterile animals
AU2013277214B2 (en) Genetically edited animals and methods for making the same
CA3003652A1 (en) Compositions and methods for chimeric embryo-assisted organ production
US10920242B2 (en) Non-meiotic allele introgression
US20190254266A1 (en) Engineering of Humanized Kidney by Genetic Complementation
US20210037797A1 (en) Inducible disease models methods of making them and use in tissue complementation
HK1235224A1 (en) Multiplex gene editing in swine
NZ715540B2 (en) Genetically sterile animals

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20161122

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
PC1203 Withdrawal of no request for examination