AR100727A1 - Sistemas y métodos para la genotipificación de material vegetal - Google Patents

Sistemas y métodos para la genotipificación de material vegetal

Info

Publication number
AR100727A1
AR100727A1 ARP150101766A ARP150101766A AR100727A1 AR 100727 A1 AR100727 A1 AR 100727A1 AR P150101766 A ARP150101766 A AR P150101766A AR P150101766 A ARP150101766 A AR P150101766A AR 100727 A1 AR100727 A1 AR 100727A1
Authority
AR
Argentina
Prior art keywords
methods
plant
embryos
detached
embryo
Prior art date
Application number
ARP150101766A
Other languages
English (en)
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of AR100727A1 publication Critical patent/AR100727A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

La solicitud proporciona métodos para obtener material genético a partir de embriones de la planta mientras que se conserva su viabilidad así como también métodos para realizar un análisis molecular de los embriones de la planta, particularmente con pequeñas cantidades de material genético. Los métodos pueden incluir las etapas de recolectar material celular desprendido de uno o más embriones de la planta; obtener ADN del material celular desprendido; realizar un análisis molecular del ADN; y germinar al menos uno de dicho uno o más embriones de la planta. Una extensión adicional de este método incluye determinar si germinar y cultivar el embrión o descartar el embrión basado en su genotipo como parte de un proceso de fitomejoramiento. Además se proporciona métodos de genotipificación de embriones mediante el uso de material embrionario celular desprendido contenido en o sobre agar y métodos para analizar el tejido embrionario de la planta derivado de microesporas.
ARP150101766A 2014-08-29 2015-06-04 Sistemas y métodos para la genotipificación de material vegetal AR100727A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US14/473,114 US11111548B2 (en) 2014-08-29 2014-08-29 Systems and methods for genotyping seed components

Publications (1)

Publication Number Publication Date
AR100727A1 true AR100727A1 (es) 2016-10-26

Family

ID=53404944

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP150101766A AR100727A1 (es) 2014-08-29 2015-06-04 Sistemas y métodos para la genotipificación de material vegetal
ARP150102751A AR101708A1 (es) 2014-08-29 2015-08-27 Métodos y dispositivos que incluyen matrices de aceite para almacenar, tratar y seleccionar embriones de plantas

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP150102751A AR101708A1 (es) 2014-08-29 2015-08-27 Métodos y dispositivos que incluyen matrices de aceite para almacenar, tratar y seleccionar embriones de plantas

Country Status (10)

Country Link
US (1) US11111548B2 (es)
EP (1) EP3186388B1 (es)
CN (1) CN106715716A (es)
AR (2) AR100727A1 (es)
AU (1) AU2015307256B2 (es)
BR (1) BR112017003935B1 (es)
CA (1) CA2954970C (es)
ES (1) ES2732754T3 (es)
MX (1) MX368327B (es)
WO (1) WO2016032589A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10945390B2 (en) 2015-07-09 2021-03-16 Syngenta Participations Ag Method of selecting corn embryos
US11890750B2 (en) 2016-05-06 2024-02-06 Pioneer Hi-Bred International, Inc. Systems and methods for maize embryo plating and replating
US20180016645A1 (en) * 2016-07-15 2018-01-18 Pioneer Hi-Bred International, Inc. Obtaining the genotype of a male gametic cell
US11873919B1 (en) * 2021-09-03 2024-01-16 Knight, Llc Actuation ball valve

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2649860A1 (fr) * 1989-07-18 1991-01-25 Nestle Sa Procede de conservation d'embryons vegetaux
US5942425A (en) * 1996-03-12 1999-08-24 Walters; Adriann H. Method to access nucleic acids from cells
US20020188965A1 (en) * 2001-04-20 2002-12-12 Zou-Yu Zhao Methods of transforming plants
CN101398428B (zh) * 2002-11-07 2012-09-05 三菱化学美迪恩斯株式会社 用于收集磁性颗粒的磁性材料及其应用
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
US7703238B2 (en) 2004-08-26 2010-04-27 Monsanto Technology Llc Methods of seed breeding using high throughput nondestructive seed sampling
US7832143B2 (en) 2004-08-26 2010-11-16 Monsanto Technology Llc High throughput methods for sampling seeds
US20100184152A1 (en) * 2006-10-23 2010-07-22 Vladislav Sandler Target-oriented whole genome amplification of nucleic acids
US7915006B2 (en) * 2006-11-13 2011-03-29 Pioneer Hi-Bred International, Inc. Methodologies, processes and automated devices for the orientation, sampling and collection of seed tissues from individual seed
EP2316037A2 (en) 2008-08-22 2011-05-04 Pioneer Hi-Bred International, Inc. Apparatus for removal of specific seed tissue or structure for seed analysis
US20100167376A1 (en) * 2008-10-06 2010-07-01 Genvault Corporation Methods for providing cellular lysates from cell wall-containing samples
US8916383B2 (en) 2009-08-12 2014-12-23 Pioneer Hi Bred International Inc Apparatus and methods to gain access to and extract intact immature embryos from developing maize kernels or specific internal tissue or structures from one or more seeds
EP2552185A4 (en) * 2010-03-26 2017-12-27 Syngenta Participations AG Tissue separation method
GB201009998D0 (en) * 2010-06-15 2010-07-21 Bg Res Cell disruption
EP2671948A1 (en) * 2012-06-07 2013-12-11 Rijk Zwaan Zaadteelt en Zaadhandel B.V. Method for analysing maternal dna in large plant populations
HUE039679T2 (hu) 2013-06-03 2019-01-28 Syngenta Participations Ag Roncsolásmentes DNS-izoláció kukoricamagokból
US20150191771A1 (en) * 2014-01-09 2015-07-09 Syngenta Participations Ag Embryo sampling method
ES2857005T3 (es) 2014-01-09 2021-09-28 Syngenta Participations Ag Método de muestreo de embriones

Also Published As

Publication number Publication date
AU2015307256B2 (en) 2021-09-09
CN106715716A (zh) 2017-05-24
AU2015307256A1 (en) 2017-02-02
ES2732754T3 (es) 2019-11-25
US11111548B2 (en) 2021-09-07
BR112017003935A2 (pt) 2018-03-06
CA2954970A1 (en) 2016-03-03
US20160060713A1 (en) 2016-03-03
AR101708A1 (es) 2017-01-04
BR112017003935B1 (pt) 2023-03-21
WO2016032589A1 (en) 2016-03-03
EP3186388B1 (en) 2019-05-22
EP3186388A1 (en) 2017-07-05
MX2017002533A (es) 2017-05-23
MX368327B (es) 2019-09-27
CA2954970C (en) 2022-08-23

Similar Documents

Publication Publication Date Title
AR100877A1 (es) Recipiente para cultivo de plantas
AR100216A1 (es) Edición de genes multiplexados
AR085533A1 (es) Metodos de produccion de un locus de rasgos transgenicos complejo
WO2013181549A3 (en) In vitro embryo blastocyst prediction methods
AR100727A1 (es) Sistemas y métodos para la genotipificación de material vegetal
CL2018001619A1 (es) Composiciones basadas en chlorella seca y métodos para el mejoramiento de las plantas.
CL2016001734A1 (es) Método de muestreo de embriones vegetales no destructivo que conserva la viabilidad, estrategia para seguir el progreso de las plantas en función de los datos, así como un método para seleccionar embriones haploides.
CO2019007828A2 (es) Evento de élite ee-gm5 y métodos y kits para identificar dicho evento en muestras biológicas
CO7101232A2 (es) Metodos para la obtencion de plantas de palma aceitera altamente productivas
BR112016021044A2 (pt) método de detecção de um perfil do marcador
WO2014063016A8 (en) Methods for generating genetically superior animals
AR101049A1 (es) Plantas de brassica que comprenden alelos mutantes de da1
AR098660A1 (es) Composiciones y métodos para seleccionar plantas de maíz resistentes a la podredumbre del tallo por antracnosis
MX368325B (es) Metodos y dispositivos que implican matrices de aceite.
MX2017002532A (es) Almacenamiento y manipulacion de embriones vegetales.
CO2019007826A2 (es) Evento de élite ee-gm4 y métodos y kits para identificar dicho evento en muestras biológicas
MX2016011428A (es) Metodos y sistemas para extraer embriones monocotiledoneos.
PE20161401A1 (es) Extracto de cascarillas agricolas efectivo para modificar el metabolismo de las plantas
EP2740350A3 (en) Methods and compositions for watermelon sex expression
ES2547032B1 (es) Método para aumentar la resistencia a los patógenos en las plantas
ECSP19027605A (es) Protocolo para la criopreservación de muestras biológicas de alta viscosidad
AR098760A1 (es) Procedimiento mediado por rhizobiaceae libre de marcadores de selección para la producción de una planta transgénica del género triticum
CL2018003204A1 (es) Métodos para optimizar la producción de metabolitos en plantas genéticamente modificadas y para procesar estas plantas.
CL2018002071A1 (es) Loci genéticos asociados con resistencia a la podredumbre parda del tallo en la soja y métodos de uso
CO2017006535A2 (es) Supresión por iarn parental del gen kruppel para el control de las plagas de hemípteros