MX368327B - Sistemas y métodos para el genotipado de material vegetal. - Google Patents

Sistemas y métodos para el genotipado de material vegetal.

Info

Publication number
MX368327B
MX368327B MX2017002533A MX2017002533A MX368327B MX 368327 B MX368327 B MX 368327B MX 2017002533 A MX2017002533 A MX 2017002533A MX 2017002533 A MX2017002533 A MX 2017002533A MX 368327 B MX368327 B MX 368327B
Authority
MX
Mexico
Prior art keywords
methods
embryos
embryo
plant
genotyping
Prior art date
Application number
MX2017002533A
Other languages
English (en)
Other versions
MX2017002533A (es
Inventor
Arnold Randal
Paul Cope Matthew
Andrew Schares Justin
Yun Yue
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of MX2017002533A publication Critical patent/MX2017002533A/es
Publication of MX368327B publication Critical patent/MX368327B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente invención se refiere a métodos para obtener material genético de embriones de plantas, a la vez que se mantiene la viabilidad, así como métodos para llevar a cabo un análisis molecular de embriones de plantas, en particular con pequeñas cantidades de material genético. Los métodos pueden incluir las etapas de recolección de material celular desprendido de uno o más embriones de plantas; obtener ADN del material celular desprendido; realizar un análisis molecular del ADN; y germinar al menos uno de esos uno o más embriones de plantas. Una extensión adicional de este método incluye determinar si el embrión germina y crecen o se lo desecha en base a su genotipo como parte de un proceso de reproducción. Se proporcionan, además, métodos de genotipado de embriones con el uso de material celular desprendido contenidas en o sobre agar, así como métodos para analizar tejido embrionario de plantas derivado de microsporas.
MX2017002533A 2014-08-29 2015-06-04 Sistemas y métodos para el genotipado de material vegetal. MX368327B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/473,114 US11111548B2 (en) 2014-08-29 2014-08-29 Systems and methods for genotyping seed components
PCT/US2015/034145 WO2016032589A1 (en) 2014-08-29 2015-06-04 Systems and methods for genotyping plant material

Publications (2)

Publication Number Publication Date
MX2017002533A MX2017002533A (es) 2017-05-23
MX368327B true MX368327B (es) 2019-09-27

Family

ID=53404944

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017002533A MX368327B (es) 2014-08-29 2015-06-04 Sistemas y métodos para el genotipado de material vegetal.

Country Status (10)

Country Link
US (1) US11111548B2 (es)
EP (1) EP3186388B1 (es)
CN (1) CN106715716A (es)
AR (2) AR100727A1 (es)
AU (1) AU2015307256B2 (es)
BR (1) BR112017003935B1 (es)
CA (1) CA2954970C (es)
ES (1) ES2732754T3 (es)
MX (1) MX368327B (es)
WO (1) WO2016032589A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10945390B2 (en) 2015-07-09 2021-03-16 Syngenta Participations Ag Method of selecting corn embryos
US11890750B2 (en) 2016-05-06 2024-02-06 Pioneer Hi-Bred International, Inc. Systems and methods for maize embryo plating and replating
US20180016645A1 (en) * 2016-07-15 2018-01-18 Pioneer Hi-Bred International, Inc. Obtaining the genotype of a male gametic cell
US11873919B1 (en) * 2021-09-03 2024-01-16 Knight, Llc Actuation ball valve

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2649860A1 (fr) * 1989-07-18 1991-01-25 Nestle Sa Procede de conservation d'embryons vegetaux
US5942425A (en) * 1996-03-12 1999-08-24 Walters; Adriann H. Method to access nucleic acids from cells
US20020188965A1 (en) * 2001-04-20 2002-12-12 Zou-Yu Zhao Methods of transforming plants
EP1580555B1 (en) * 2002-11-07 2014-02-12 Mitsubishi Chemical Medience Corporation Magnetic material for collecting magnetic particles and utilization thereof
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
US7832143B2 (en) 2004-08-26 2010-11-16 Monsanto Technology Llc High throughput methods for sampling seeds
US7703238B2 (en) 2004-08-26 2010-04-27 Monsanto Technology Llc Methods of seed breeding using high throughput nondestructive seed sampling
WO2008051928A2 (en) * 2006-10-23 2008-05-02 The Salk Institute For Biological Studies Target-oriented whole genome amplification of nucliec acids
US7915006B2 (en) * 2006-11-13 2011-03-29 Pioneer Hi-Bred International, Inc. Methodologies, processes and automated devices for the orientation, sampling and collection of seed tissues from individual seed
CA2735045A1 (en) 2008-08-22 2010-02-25 Pioneer Hi-Bred International, Inc. Apparatus for removal of specific seed tissue or structure for seed analysis
US20100167376A1 (en) * 2008-10-06 2010-07-01 Genvault Corporation Methods for providing cellular lysates from cell wall-containing samples
US8916383B2 (en) 2009-08-12 2014-12-23 Pioneer Hi Bred International Inc Apparatus and methods to gain access to and extract intact immature embryos from developing maize kernels or specific internal tissue or structures from one or more seeds
BR112012024358A2 (pt) * 2010-03-26 2016-05-24 Syngenta Participations Ag método de separação de tecido
GB201009998D0 (en) * 2010-06-15 2010-07-21 Bg Res Cell disruption
EP2671948A1 (en) * 2012-06-07 2013-12-11 Rijk Zwaan Zaadteelt en Zaadhandel B.V. Method for analysing maternal dna in large plant populations
HUE039679T2 (hu) 2013-06-03 2019-01-28 Syngenta Participations Ag Roncsolásmentes DNS-izoláció kukoricamagokból
US20150191771A1 (en) * 2014-01-09 2015-07-09 Syngenta Participations Ag Embryo sampling method
HUE053440T2 (hu) 2014-01-09 2021-06-28 Syngenta Participations Ag Embrió-mintavételezõ eljárás

Also Published As

Publication number Publication date
WO2016032589A1 (en) 2016-03-03
AR101708A1 (es) 2017-01-04
AR100727A1 (es) 2016-10-26
US11111548B2 (en) 2021-09-07
CA2954970A1 (en) 2016-03-03
BR112017003935B1 (pt) 2023-03-21
ES2732754T3 (es) 2019-11-25
US20160060713A1 (en) 2016-03-03
CA2954970C (en) 2022-08-23
CN106715716A (zh) 2017-05-24
EP3186388A1 (en) 2017-07-05
AU2015307256B2 (en) 2021-09-09
AU2015307256A1 (en) 2017-02-02
EP3186388B1 (en) 2019-05-22
BR112017003935A2 (pt) 2018-03-06
MX2017002533A (es) 2017-05-23

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