AR092588A1 - Metodo de obtencion de 1-kestosa - Google Patents

Metodo de obtencion de 1-kestosa

Info

Publication number
AR092588A1
AR092588A1 ARP130103318A ARP130103318A AR092588A1 AR 092588 A1 AR092588 A1 AR 092588A1 AR P130103318 A ARP130103318 A AR P130103318A AR P130103318 A ARP130103318 A AR P130103318A AR 092588 A1 AR092588 A1 AR 092588A1
Authority
AR
Argentina
Prior art keywords
obtaining
sucrose
recombinant
kestosa
kestose
Prior art date
Application number
ARP130103318A
Other languages
English (en)
Inventor
Perez Cruz Enrique
Hernandez Garcia Lazaro
Martinez Garcia Duniesky
Enrique Trujillo Toledo Luis
Menendez Rodriguez Carmen
Sobrino Legon Alina
Ramirez Ibaez Ricardo
Feijoo Costa Gumersindo
Manuel Lema Rodicio Juan
Original Assignee
Ct Ingenieria Genetica Biotecnologia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Biotecnologia filed Critical Ct Ingenieria Genetica Biotecnologia
Publication of AR092588A1 publication Critical patent/AR092588A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01099—Sucrose:sucrose fructosyltransferase (2.4.1.99)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Physiology (AREA)

Abstract

La presente revela un método de obtención de 1-kestosa, a escala industrial, mediante el empleo de una fructosiltransferasa (FTF) recombinante, aislada de Festuca arundinacea, expresada constitutivamente en una levadura hospedera no sacarolítica. La FTF recombinante tipo sacarosa: sacarosa 1-fructosiltransferasa (1-SSTrec) se produce de forma constitutiva, estable y en altos niveles, tanto en el sobrenadante como en el sedimento celular del cultivo de una cepa de Pichia pastoris. Por tanto, también se provee un método para la obtención de 1-SST a escala industrial. La enzima recombinante resultante se emplea para la producción enzimática masiva de fructoligosacáridos (FOS), específicamente de 1-kestosa, a partir de la sacarosa. El método permite conversiones superiores al 55% en FOS, donde la 1-kestosa está representada en más de un 90%.
ARP130103318A 2012-09-18 2013-09-16 Metodo de obtencion de 1-kestosa AR092588A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20120138A CU24158B1 (es) 2012-09-18 2012-09-18 Método de obtención de 1-kestosa

Publications (1)

Publication Number Publication Date
AR092588A1 true AR092588A1 (es) 2015-04-29

Family

ID=49474178

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130103318A AR092588A1 (es) 2012-09-18 2013-09-16 Metodo de obtencion de 1-kestosa

Country Status (18)

Country Link
US (2) US10131927B2 (es)
EP (1) EP2899282A1 (es)
JP (2) JP2015530089A (es)
KR (1) KR102097749B1 (es)
CN (1) CN104781414B (es)
AR (1) AR092588A1 (es)
AU (2) AU2013317358B2 (es)
BR (2) BR112015005852B1 (es)
CA (1) CA2885116C (es)
CL (1) CL2015000690A1 (es)
CU (1) CU24158B1 (es)
EA (1) EA028094B1 (es)
IN (1) IN2015DN02234A (es)
MX (1) MX363716B (es)
MY (1) MY190696A (es)
UA (1) UA118545C2 (es)
WO (1) WO2014044230A1 (es)
ZA (1) ZA201501833B (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2018117198A1 (ja) * 2017-04-14 2020-01-23 物産フードサイエンス株式会社 糖液ならびにこれを用いる液体甘味料およびハナバチ用飼料
JP2020146312A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146297A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146313A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146308A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146302A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146304A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146318A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146310A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146298A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146316A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146309A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146305A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146314A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146307A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146315A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146301A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146306A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146300A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146299A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146317A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146311A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
KR102402632B1 (ko) * 2019-10-31 2022-05-26 주식회사 삼양사 케스토스-함유 프락토올리고당 제조방법
CN112210576B (zh) * 2020-09-28 2023-05-16 量子高科(广东)生物有限公司 一种高纯度蔗果三糖及其制备方法
CN114438104B (zh) * 2022-03-21 2023-06-20 重庆大学 一种调控番茄果实糖含量的SlGRAS9基因及在培育高糖含量番茄中的应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4309505A (en) 1980-05-19 1982-01-05 Cpc International Inc. Process for the production of fructose transferase enzyme
CA1209932A (en) 1983-09-27 1986-08-19 Horst W. Doelle Conversion of sucrose to fructose and ethanol
NL8701616A (nl) 1987-07-09 1989-02-01 Stamicarbon Fructosyltransferase en bereiding van fructose-oligomeren daarmee.
US4927757A (en) 1988-07-29 1990-05-22 Idaho Research Foundation, Inc. Production of substantially pure fructose
JPH0670075B2 (ja) 1990-08-28 1994-09-07 ホクレン農業協同組合連合会 １―ケストース結晶およびその製造方法
AU682727B2 (en) * 1993-12-23 1997-10-16 Centro De Ingenieria Genetica Y Biotecnologia Fructosyltransferase enzyme, method for its production, and DNA encoding the enzyme
NL1000064C1 (nl) * 1994-07-08 1996-01-08 Stichting Scheikundig Onderzoe Produktie van oligosacchariden in transgene planten.
CA2243072C (en) 1995-12-11 2009-01-27 Meiji Seika Kaisha, Ltd. Crystalline 1-kestose and process for preparing the same
JP2001245697A (ja) * 2000-03-08 2001-09-11 Japan Science & Technology Corp 遺伝子発現定量法
CA2402562A1 (en) * 2000-03-16 2001-09-20 Shantha Biotechnics (P) Ltd. A process for the production of human interferon alpha from genetically engineered yeast
JP2002209462A (ja) * 2000-12-26 2002-07-30 Academia Sinica トランスジェニック植物種子におけるタンパク質産生
DE10355302A1 (de) * 2003-11-27 2005-06-23 Technische Universität München Verfahren zur fermentativen Anreicherung von Lebensmitteln mit Fructose-Oligosacchariden
EP1726655A4 (en) 2004-03-04 2007-12-12 Meiji Seika Kaisha BETA-fructofuranosidase MUTANT
JP2009240161A (ja) * 2006-10-04 2009-10-22 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
EP3399042A1 (en) * 2007-05-17 2018-11-07 Boehringer Ingelheim RCV GmbH & Co KG Method for producing a recombinant protein on a manufacturing scale
BRPI0705359B1 (pt) 2007-12-12 2017-05-02 Univ Estadual De Campinas - Unicamp processo de obtenção de frutooligossacarídeos em bioreator utilizando enzima frutosiltransferase
JP2009173548A (ja) * 2008-01-21 2009-08-06 Tokai Igaku Kensa Kenkyusho:Kk 腸内細菌叢改善組成物、アレルギー抑制組成物、およびアレルギー抑制剤
US9232813B2 (en) * 2008-07-07 2016-01-12 The Iams Company Probiotic supplement, process for making, and packaging
ES2348990B1 (es) 2009-03-11 2011-10-10 Consejo Superior De Investigaciones Cientificas (Csic) (85%) Biocatalizador inmovilizado basado en alginato para la biotransformacion de carbohidratos.
JP2010273580A (ja) 2009-05-27 2010-12-09 Meiji Seika Kaisha Ltd １−ケストースの結晶化母液の調製方法

Also Published As

Publication number Publication date
JP2019047797A (ja) 2019-03-28
UA118545C2 (uk) 2019-02-11
AU2017221776A1 (en) 2017-09-21
JP6994449B2 (ja) 2022-02-04
KR102097749B1 (ko) 2020-04-06
AU2017221776B2 (en) 2019-03-21
KR20150056795A (ko) 2015-05-27
BR122021021803B1 (pt) 2022-04-12
CA2885116C (en) 2021-09-14
EA028094B1 (ru) 2017-10-31
ZA201501833B (en) 2016-08-31
EP2899282A1 (en) 2015-07-29
CN104781414B (zh) 2019-09-03
CN104781414A (zh) 2015-07-15
IN2015DN02234A (es) 2015-08-21
BR112015005852A2 (pt) 2017-07-04
CA2885116A1 (en) 2014-03-27
US10131927B2 (en) 2018-11-20
AU2013317358A1 (en) 2015-04-02
CL2015000690A1 (es) 2015-08-07
MY190696A (en) 2022-05-11
US10982246B2 (en) 2021-04-20
MX363716B (es) 2019-03-29
JP2015530089A (ja) 2015-10-15
BR112015005852B1 (pt) 2022-03-29
EA201590599A1 (ru) 2015-07-30
AU2013317358B2 (en) 2017-06-08
WO2014044230A1 (es) 2014-03-27
US20190085364A1 (en) 2019-03-21
MX2015003497A (es) 2015-06-22
CU24158B1 (es) 2016-03-30
US20150232898A1 (en) 2015-08-20
CU20120138A7 (es) 2014-05-27

Similar Documents

Publication Publication Date Title
AR092588A1 (es) Metodo de obtencion de 1-kestosa
BR112013010353A2 (pt) célula hospedeira recombinante de lactobacillus, e, métodos para produzir isopropanol, isopropanol e n-propanol, e propileno
BR112018004377A2 (pt) cepas de levedura para a expressão e secreção de proteínas heterólogas em temperaturas elevadas
WO2013076144A3 (en) Microorganism strains for the production of 2,3-butanediol
MY173141A (en) Glycerol and acetic acid converting yeast cells with improved acetic acid conversion
MX2012013037A (es) Composiciones de endorribonucleasa y metodos de uso de las mismas.
BR112017021255A2 (pt) microrganismo modificado para a produção otimizada de 2,4-di-hidroxibutirato
MX352023B (es) Variantes de alfa-amilasa con estabilidad mejorada.
BR112016016524A2 (pt) célula de levedura transformada
MY172288A (en) Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases
IN2015DN01365A (es)
EA201592169A1 (ru) Рекомбинантные микроорганизмы, приводящие к увеличению потока через путь ферментации
MY164858A (en) Microorganisms of corynebacterium which can utilize xylose and method for producing l-lysine using same
WO2016071505A9 (en) Microbial production of the flavonoids garbanzol, resokaempferol and fisetin
BR112012020503A2 (pt) variante isolado de celobioidrolase precursor, polipeptídeo isolado, construção de ácido nucleico, vetor de expressão, célula hospedeira, método de produzir um variante de celobioidrolas precursor, métodos para obter o variante, para degradar variante de celobioidrolase precursor, métodos para obter o variante, para degradar ou converter um material celulósico, poara produzir um produto de fermentação e de fermentar um material celulósico, e, composição de enzima.
WO2015155791A3 (en) Production of succinic acid from organic waste or biogas or methane using recombinant methanotrophic bacterium
WO2014120852A3 (en) Recombinant microorganisms comprising nadph dependent enzymes and methods of production thereof
MX2016002616A (es) Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados.
CA2886020C (en) Mutant yeast strain with decreased glycerol production
WO2013182553A3 (en) CELL ENGINEERING USING RNAs
WO2014049382A3 (en) Ethylenediamine fermentative production by a recombinant microorganism
MX2013009403A (es) Agrupamiento genico para la biosintesis de cicloclavina.
EP2480657A4 (en) METHOD OF EFFICIENTLY ESTABLISHING A LINE OF INDUCED PLURIPOTENT STEM CELLS (SPI)
BR112014015566B8 (pt) Célula hospedeira bacteriana recombinante e processo para produção de etanol
WO2016069155A3 (en) Method for biosynthesis of acetaminophen

Legal Events

Date Code Title Description
FG Grant, registration