BR112013010353A2 - célula hospedeira recombinante de lactobacillus, e, métodos para produzir isopropanol, isopropanol e n-propanol, e propileno - Google Patents

célula hospedeira recombinante de lactobacillus, e, métodos para produzir isopropanol, isopropanol e n-propanol, e propileno

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Publication number
BR112013010353A2
BR112013010353A2 BR112013010353A BR112013010353A BR112013010353A2 BR 112013010353 A2 BR112013010353 A2 BR 112013010353A2 BR 112013010353 A BR112013010353 A BR 112013010353A BR 112013010353 A BR112013010353 A BR 112013010353A BR 112013010353 A2 BR112013010353 A2 BR 112013010353A2
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BR
Brazil
Prior art keywords
isopropanol
propanol
methods
producing
propylene
Prior art date
Application number
BR112013010353A
Other languages
English (en)
Inventor
Alan Berry
Bjarke Christensen
Steen Troels Joergensen
Thomas Grotkjaer
Torsten Bak Regueira
Original Assignee
Novozymes As
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As, Novozymes Inc filed Critical Novozymes As
Publication of BR112013010353A2 publication Critical patent/BR112013010353A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/0108—Isopropanol dehydrogenase (NADP+) (1.1.1.80)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01003—Aldehyde dehydrogenase (NAD+) (1.2.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01009—Acetyl-CoA C-acetyltransferase (2.3.1.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/03—CoA-transferases (2.8.3)
    • C12Y208/03005—3-Oxoacid CoA-transferase (2.8.3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/03—CoA-transferases (2.8.3)
    • C12Y208/03009—Butyrate--acetoacetate CoA-transferase (2.8.3.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02011—Acetoacetyl-CoA hydrolase (3.1.2.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01004—Acetoacetate decarboxylase (4.1.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01041—Methylmalonyl-CoA decarboxylase (4.1.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/99—Racemaces and epimerases (5.1) acting on other compounds (5.1.99)
    • C12Y501/99001—Methylmalonyl-CoA epimerase (5.1.99.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99002—Methylmalonyl-CoA mutase (5.4.99.2)

Abstract

célula hospedeira recombinante de lactobacillus, e, métodos para produzir isopropanol, isopropanol e n-propanol, e propileno. a presente invenção se refere aos métodos de produzir inpropanol, isopropanol, e coproduzir n-propanol com isopropanol. a presente invenção também se refere aos métosos para produzir propileno, bem como células hospedeiras capazes de produzir n-propanol e isopropanol.
BR112013010353A 2010-10-29 2011-10-28 célula hospedeira recombinante de lactobacillus, e, métodos para produzir isopropanol, isopropanol e n-propanol, e propileno BR112013010353A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US40814610P 2010-10-29 2010-10-29
US40813810P 2010-10-29 2010-10-29
US40815410P 2010-10-29 2010-10-29
PCT/US2011/058405 WO2012058603A1 (en) 2010-10-29 2011-10-28 Recombinant n-propanol and isopropanol production

Publications (1)

Publication Number Publication Date
BR112013010353A2 true BR112013010353A2 (pt) 2016-07-05

Family

ID=44913441

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013010353A BR112013010353A2 (pt) 2010-10-29 2011-10-28 célula hospedeira recombinante de lactobacillus, e, métodos para produzir isopropanol, isopropanol e n-propanol, e propileno

Country Status (9)

Country Link
US (1) US20130280775A1 (pt)
EP (1) EP2633030A1 (pt)
JP (1) JP2013544083A (pt)
CN (1) CN103314100A (pt)
AU (1) AU2011320329B2 (pt)
BR (1) BR112013010353A2 (pt)
CA (1) CA2810903A1 (pt)
MX (1) MX2013004353A (pt)
WO (1) WO2012058603A1 (pt)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014002405A2 (pt) 2011-08-04 2017-04-04 Novozymes As polopeptídeo e polinucleotídeo isolados, composição, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, planta transgênica, parte da planta ou célula de planta, métodos para produzir um polipeptídeo e uma proteína, e, processos para degradar um material celulóssico, para produzir um produto de fermentação, e para fermentar um material celulósico
US9890398B2 (en) 2011-10-24 2018-02-13 Toyota Jidosha Kabushiki Kaisha Method for producing ethanol using recombinant yeast
WO2013173711A1 (en) 2012-05-18 2013-11-21 Novozymes A/S Bacterial mutants with improved transformation efficiency
WO2013178699A1 (en) 2012-05-31 2013-12-05 Novozymes A/S Isopropanol production by bacterial hosts
US20150218567A1 (en) 2012-09-27 2015-08-06 Novozymes A/S Bacterial Mutants with Improved Transformation Efficiency
WO2014068010A1 (en) 2012-10-31 2014-05-08 Novozymes A/S Isopropanol production by bacterial hosts
WO2014076232A2 (en) 2012-11-19 2014-05-22 Novozymes A/S Isopropanol production by recombinant hosts using an hmg-coa intermediate
CN105073990A (zh) * 2013-02-27 2015-11-18 丰田自动车株式会社 使用了重组酵母的乙醇的制造方法
WO2015035226A2 (en) * 2013-09-05 2015-03-12 Braskem S/A Modified microorganism and methods of using same for producing 2- propanol and 1-propanol and/or 1.2-propanediol
EP3536799A3 (en) * 2013-09-23 2020-12-09 Braskem S.A. Engineered enzyme having acetoacetyl-coa hydrolase activity, microorganisms comprising same, and methods of using same
CN103923871A (zh) * 2014-05-08 2014-07-16 北京化工大学 导入异源代谢途径的产1-丙醇微生物及使用所述微生物生产1-丙醇的方法
CN109312373B (zh) 2016-03-09 2022-07-19 布拉斯肯有限公司 用于共生产乙二醇和三碳化合物的微生物和方法
BR112019013007A2 (pt) 2016-12-21 2019-10-08 Creatus Biosciences Inc organismo microbiano não natural, método de produção de um composto bioderivado, composto bioderivado e composição
JP6879111B2 (ja) 2017-08-02 2021-06-02 トヨタ自動車株式会社 組換え酵母及びこれを用いたエタノールの製造方法
MX2022010298A (es) 2020-02-21 2022-09-19 Braskem Sa Produccion de etanol con uno o mas coproductos en levadura.
US20240067994A1 (en) 2022-08-24 2024-02-29 Braskem S.A. Process for the recovery of low-boiling point components from an ethanol stream

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
ES2165420T3 (es) 1994-06-03 2002-03-16 Novozymes Biotech Inc Lacasas myceliophthora purificadas y acidos nucleicos que las codifican.
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
DE69932345T2 (de) 1998-10-26 2007-07-19 Novozymes A/S Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
CN100482801C (zh) 1999-03-22 2009-04-29 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
MX2009008416A (es) * 2007-02-09 2010-01-25 Univ California Produccion de biocombustible mediante microorganismos recombinantes.
BRPI0816161A2 (pt) * 2007-08-29 2014-10-14 Res Inst Innovative Tech Earth Transformante capaz de produzir isopropanol
EP2198039A4 (en) 2007-10-12 2012-05-09 Univ California MODIFIED MICROORGANISM FOR PRODUCING ISOPROPANOL
SG186646A1 (en) * 2007-12-13 2013-01-30 Glycos Biotechnologies Inc Microbial conversion of oils and fatty acids to high-value chemicals
WO2009103026A1 (en) * 2008-02-15 2009-08-20 Gevo, Inc. Engineered microorganisms for producing isopropanol
EP2319223A1 (en) 2008-04-24 2011-05-11 SK Telecom Co., Ltd. Scalable video providing and reproducing system and methods thereof
AU2009327490A1 (en) 2008-12-16 2011-07-28 Genomatica, Inc. Microorganisms and methods for conversion of syngas and other carbon sources to useful products
AU2010284110B2 (en) 2009-08-21 2015-10-29 Lallemand Hungary Liquidity Management Llc Production of propanols, alcohols, and polyols in consolidated bioprocessing organisms
MX2012002995A (es) 2009-09-09 2012-08-23 Braskem Sa Microorganismos y proceso para producir n-propanol.
BR112012005296A2 (pt) * 2009-09-09 2019-01-15 Genomatica Inc microorganismos e métodos para a coprodução de isopropanol com alcoóis primários, diós e ácidos.

Also Published As

Publication number Publication date
JP2013544083A (ja) 2013-12-12
AU2011320329B2 (en) 2015-03-12
CA2810903A1 (en) 2012-05-03
MX2013004353A (es) 2013-06-28
US20130280775A1 (en) 2013-10-24
AU2011320329A1 (en) 2013-03-28
CN103314100A (zh) 2013-09-18
EP2633030A1 (en) 2013-09-04
WO2012058603A1 (en) 2012-05-03

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