IN2015DN02234A - - Google Patents

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Publication number
IN2015DN02234A
IN2015DN02234A IN2234DEN2015A IN2015DN02234A IN 2015DN02234 A IN2015DN02234 A IN 2015DN02234A IN 2234DEN2015 A IN2234DEN2015 A IN 2234DEN2015A IN 2015DN02234 A IN2015DN02234 A IN 2015DN02234A
Authority
IN
India
Prior art keywords
kestose
sucrose
recombinant
fos
fructosyltransferase
Prior art date
Application number
Inventor
Cruz Enrique Rosendo; Pérez
García Lázaro; Hernández
García Duniesky; Martínez
Rodríguez Carmen; Menéndez
Legón Alina; Sobrino
Ibañez Ricardo; Ramírez
Costa Gumersindo; Feijoo
Rodicio Juan Manuel; Lema
Original Assignee
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech
Publication of IN2015DN02234A publication Critical patent/IN2015DN02234A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01099—Sucrose:sucrose fructosyltransferase (2.4.1.99)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Physiology (AREA)

Abstract

The invention relates to a method for obtaining 1- kestose on an industrial scale , using a recombinant fructosyltransferase (FTF) isolated from Festuca arundinacea , constitutively expressed in a non saccharolytic yeast host. According to the invention , the recombinant FTF of the sucrose:sucrose 1 -fructosyltransferase (1 SSTrec) type is produced in a constitutive and stable manner and in high yields, both in the supernatant and in the cell sediment of the culture of a strain of . The invention also relates to a method for obtaining 1- SST on an industrial scale. The resulting recombinant enzyme is used for the mass enzymatic production of fructooligosaccharides (FOS), specifically 1- kestose , from sucrose. The method of the invention allows FOS conversion percentages greater than 55% , with 1- kestose being represented in more than 90%.
IN2234DEN2015 2012-09-18 2013-09-18 IN2015DN02234A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU20120138A CU24158B1 (en) 2012-09-18 2012-09-18 1-KESTOSA OBTAINING METHOD
PCT/CU2013/000005 WO2014044230A1 (en) 2012-09-18 2013-09-18 Method for obtaining 1-kestose

Publications (1)

Publication Number Publication Date
IN2015DN02234A true IN2015DN02234A (en) 2015-08-21

Family

ID=49474178

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2234DEN2015 IN2015DN02234A (en) 2012-09-18 2013-09-18

Country Status (18)

Country Link
US (2) US10131927B2 (en)
EP (1) EP2899282A1 (en)
JP (2) JP2015530089A (en)
KR (1) KR102097749B1 (en)
CN (1) CN104781414B (en)
AR (1) AR092588A1 (en)
AU (2) AU2013317358B2 (en)
BR (2) BR112015005852B1 (en)
CA (1) CA2885116C (en)
CL (1) CL2015000690A1 (en)
CU (1) CU24158B1 (en)
EA (1) EA028094B1 (en)
IN (1) IN2015DN02234A (en)
MX (1) MX363716B (en)
MY (1) MY190696A (en)
UA (1) UA118545C2 (en)
WO (1) WO2014044230A1 (en)
ZA (1) ZA201501833B (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2018117198A1 (en) * 2017-04-14 2020-01-23 物産フードサイエンス株式会社 Sugar solution and feed for liquid sweetener and bee using the same
JP2020146312A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146297A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146313A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146308A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146302A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146304A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146318A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146310A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146298A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146316A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146309A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146305A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146314A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146307A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146315A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146301A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146306A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146300A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146299A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146317A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
JP2020146311A (en) * 2019-03-14 2020-09-17 株式会社三洋物産 Game machine
KR102402632B1 (en) * 2019-10-31 2022-05-26 주식회사 삼양사 A method of preparing fructooligosaccharides comprising kestose
CN112210576B (en) * 2020-09-28 2023-05-16 量子高科(广东)生物有限公司 High-purity kestose and preparation method thereof
CN114438104B (en) * 2022-03-21 2023-06-20 重庆大学 SlGRAS9 gene for regulating and controlling sugar content of tomato fruits and application of SlGRAS9 gene in cultivation of tomatoes with high sugar content

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CA1209932A (en) 1983-09-27 1986-08-19 Horst W. Doelle Conversion of sucrose to fructose and ethanol
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DE10355302A1 (en) * 2003-11-27 2005-06-23 Technische Universität München Process for fermentative fortification of foods with fructose oligosaccharides
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JP2009240161A (en) * 2006-10-04 2009-10-22 Ajinomoto Co Inc Method for producing l-amino acid
EP3399042A1 (en) * 2007-05-17 2018-11-07 Boehringer Ingelheim RCV GmbH & Co KG Method for producing a recombinant protein on a manufacturing scale
BRPI0705359B1 (en) 2007-12-12 2017-05-02 Univ Estadual De Campinas - Unicamp process of obtaining fructooligosaccharides in bioreactor using fructosyltransferase enzyme
JP2009173548A (en) * 2008-01-21 2009-08-06 Tokai Igaku Kensa Kenkyusho:Kk Intestinal bacterium flora-improving composition, allergy-inhibiting composition, and allergy inhibitor
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Also Published As

Publication number Publication date
JP2019047797A (en) 2019-03-28
UA118545C2 (en) 2019-02-11
AU2017221776A1 (en) 2017-09-21
JP6994449B2 (en) 2022-02-04
KR102097749B1 (en) 2020-04-06
AU2017221776B2 (en) 2019-03-21
KR20150056795A (en) 2015-05-27
BR122021021803B1 (en) 2022-04-12
CA2885116C (en) 2021-09-14
EA028094B1 (en) 2017-10-31
ZA201501833B (en) 2016-08-31
AR092588A1 (en) 2015-04-29
EP2899282A1 (en) 2015-07-29
CN104781414B (en) 2019-09-03
CN104781414A (en) 2015-07-15
BR112015005852A2 (en) 2017-07-04
CA2885116A1 (en) 2014-03-27
US10131927B2 (en) 2018-11-20
AU2013317358A1 (en) 2015-04-02
CL2015000690A1 (en) 2015-08-07
MY190696A (en) 2022-05-11
US10982246B2 (en) 2021-04-20
MX363716B (en) 2019-03-29
JP2015530089A (en) 2015-10-15
BR112015005852B1 (en) 2022-03-29
EA201590599A1 (en) 2015-07-30
AU2013317358B2 (en) 2017-06-08
WO2014044230A1 (en) 2014-03-27
US20190085364A1 (en) 2019-03-21
MX2015003497A (en) 2015-06-22
CU24158B1 (en) 2016-03-30
US20150232898A1 (en) 2015-08-20
CU20120138A7 (en) 2014-05-27

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