BR112015005852A2 - método para obtenção de 1-questose - Google Patents

método para obtenção de 1-questose

Info

Publication number
BR112015005852A2
BR112015005852A2 BR112015005852A BR112015005852A BR112015005852A2 BR 112015005852 A2 BR112015005852 A2 BR 112015005852A2 BR 112015005852 A BR112015005852 A BR 112015005852A BR 112015005852 A BR112015005852 A BR 112015005852A BR 112015005852 A2 BR112015005852 A2 BR 112015005852A2
Authority
BR
Brazil
Prior art keywords
sucrose
fos
questose
obtaining
fructosyltransferase
Prior art date
Application number
BR112015005852A
Other languages
English (en)
Other versions
BR112015005852B1 (pt
Inventor
Sobrino Legón Alina
Menendez Rodriguez Carmen
Martinez Garcia Duniesky
Rosendo Perez Cruz Enrique
Feijoo Costa Gumersindo
Manuel Lema Rodicio Juan
Enrique Trujillo Toledo Luis
Hernandez Garcia Lázaro
Ramires Ibanez Ricardo
Original Assignee
Ct Ingenieria Genetica Biotecnologia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Biotecnologia filed Critical Ct Ingenieria Genetica Biotecnologia
Priority to BR122021021803-0A priority Critical patent/BR122021021803B1/pt
Publication of BR112015005852A2 publication Critical patent/BR112015005852A2/pt
Publication of BR112015005852B1 publication Critical patent/BR112015005852B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01099—Sucrose:sucrose fructosyltransferase (2.4.1.99)

Abstract

abstract the present invention discloses an industrial scale method to obtain 1-kestose by the use of a recombinant fructosyltransferase (ftf), isolated from festuca arundinacea, expressed constitutively in a non-saccharolytic yeast. in this invention, the recombinant ftf type sucrose:sucrose 1-fructosyltransferase (1-sstrec) is produced constitutively, stable and at high yield, both in the culture supernatant and in intact cells of the host pichia pastoris. hence, the invention additionally provides a method for 1-sst production at industrial scale. the recombinant enzyme is then used for mass production of short-chain fructooligosaccharides (fos), specifically 1-kestose, from sucrose. the method of the present invention establishes conditions that allow conversion rates where the synthesized fos constitute above 55% (w/w) of the total sugars in the reaction mixture and the 1-kestose content reaches values higher than 90% (w/w) of the total fos fraction. tradução do resumo resumo patente de invenção: "método para obtenção de 1-questose". a presente invenção divulga um método de obtenção de 1-questose, em escala industrial, mediante o uso de uma fructosiltransferase recombinante (ftf), isolada de festuca arundinacea, expressa constitutivamente em uma levedura hospedeira não sacarolítica. na invenção, a ftf recombinante tipo sacarose:sacarose 1-fructosiltransferase (1-sstrec) é produzida de forma constitutiva, estável e em níveis elevados, tanto no sobrenadante quanto no sedimento celular da cultura de uma cepa de pichia pastoris. portanto, a invenção também oferece um método para a obtenção de 1-sst em escala industrial. a enzima recombinante resultante é então empregada para a produção enzimática em massa de fruto-oligossacarídeos (fos), especificamente 1-questose, a partir de sacarose. o método da invenção permite conversões em fos superiores a 55%, com a 1-questose estando representada em mais de 90%.
BR112015005852-3A 2012-09-18 2013-09-18 Método para a produção de 1-sst em escala industrial BR112015005852B1 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR122021021803-0A BR122021021803B1 (pt) 2012-09-18 2013-09-18 Método para a produção de 1-questose em escala industrial

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CU2012-0138 2012-09-18
CU20120138A CU24158B1 (es) 2012-09-18 2012-09-18 Método de obtención de 1-kestosa
PCT/CU2013/000005 WO2014044230A1 (es) 2012-09-18 2013-09-18 Método de obtención de 1-kestosa

Publications (2)

Publication Number Publication Date
BR112015005852A2 true BR112015005852A2 (pt) 2017-07-04
BR112015005852B1 BR112015005852B1 (pt) 2022-03-29

Family

ID=49474178

Family Applications (2)

Application Number Title Priority Date Filing Date
BR122021021803-0A BR122021021803B1 (pt) 2012-09-18 2013-09-18 Método para a produção de 1-questose em escala industrial
BR112015005852-3A BR112015005852B1 (pt) 2012-09-18 2013-09-18 Método para a produção de 1-sst em escala industrial

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR122021021803-0A BR122021021803B1 (pt) 2012-09-18 2013-09-18 Método para a produção de 1-questose em escala industrial

Country Status (18)

Country Link
US (2) US10131927B2 (pt)
EP (1) EP2899282A1 (pt)
JP (2) JP2015530089A (pt)
KR (1) KR102097749B1 (pt)
CN (1) CN104781414B (pt)
AR (1) AR092588A1 (pt)
AU (2) AU2013317358B2 (pt)
BR (2) BR122021021803B1 (pt)
CA (1) CA2885116C (pt)
CL (1) CL2015000690A1 (pt)
CU (1) CU24158B1 (pt)
EA (1) EA028094B1 (pt)
IN (1) IN2015DN02234A (pt)
MX (1) MX363716B (pt)
MY (1) MY190696A (pt)
UA (1) UA118545C2 (pt)
WO (1) WO2014044230A1 (pt)
ZA (1) ZA201501833B (pt)

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JP2020146307A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146300A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146302A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146306A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146318A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146313A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146310A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146315A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146298A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146299A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146316A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146308A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146304A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146305A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146297A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146301A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146314A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146309A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146311A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146317A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146312A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
KR102402632B1 (ko) * 2019-10-31 2022-05-26 주식회사 삼양사 케스토스-함유 프락토올리고당 제조방법
CN112210576B (zh) * 2020-09-28 2023-05-16 量子高科(广东)生物有限公司 一种高纯度蔗果三糖及其制备方法
CN114438104B (zh) * 2022-03-21 2023-06-20 重庆大学 一种调控番茄果实糖含量的SlGRAS9基因及在培育高糖含量番茄中的应用

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Also Published As

Publication number Publication date
MX363716B (es) 2019-03-29
BR112015005852B1 (pt) 2022-03-29
EA028094B1 (ru) 2017-10-31
WO2014044230A1 (es) 2014-03-27
JP2015530089A (ja) 2015-10-15
CN104781414A (zh) 2015-07-15
BR122021021803B1 (pt) 2022-04-12
AU2017221776B2 (en) 2019-03-21
US10131927B2 (en) 2018-11-20
CA2885116A1 (en) 2014-03-27
MX2015003497A (es) 2015-06-22
AU2013317358A1 (en) 2015-04-02
EP2899282A1 (en) 2015-07-29
JP2019047797A (ja) 2019-03-28
JP6994449B2 (ja) 2022-02-04
CN104781414B (zh) 2019-09-03
EA201590599A1 (ru) 2015-07-30
IN2015DN02234A (pt) 2015-08-21
KR102097749B1 (ko) 2020-04-06
CU20120138A7 (es) 2014-05-27
US20190085364A1 (en) 2019-03-21
US20150232898A1 (en) 2015-08-20
UA118545C2 (uk) 2019-02-11
CA2885116C (en) 2021-09-14
CL2015000690A1 (es) 2015-08-07
AU2017221776A1 (en) 2017-09-21
CU24158B1 (es) 2016-03-30
AU2013317358B2 (en) 2017-06-08
ZA201501833B (en) 2016-08-31
KR20150056795A (ko) 2015-05-27
MY190696A (en) 2022-05-11
AR092588A1 (es) 2015-04-29
US10982246B2 (en) 2021-04-20

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B15K Others concerning applications: alteration of classification

Ipc: C12P 19/18 (2006.01), A23L 2/60 (2006.01)

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 18/09/2013, OBSERVADAS AS CONDICOES LEGAIS.