BR112016016524A2 - célula de levedura transformada - Google Patents

célula de levedura transformada

Info

Publication number
BR112016016524A2
BR112016016524A2 BR112016016524-1A BR112016016524A BR112016016524A2 BR 112016016524 A2 BR112016016524 A2 BR 112016016524A2 BR 112016016524 A BR112016016524 A BR 112016016524A BR 112016016524 A2 BR112016016524 A2 BR 112016016524A2
Authority
BR
Brazil
Prior art keywords
yeast cell
transformed yeast
yeast
phosphoribulokinase
recreates
Prior art date
Application number
BR112016016524-1A
Other languages
English (en)
Inventor
Pompon Denis
Pâques Frédéric
Lesage Julie
Guillouet Stèphane
Bonnot Florence
Marc Jillian
Gorret Nathalie
Bideaux Carine
Boutonnet Christel
Original Assignee
Institut National De La Recherche Agronomique
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National De La Recherche Agronomique filed Critical Institut National De La Recherche Agronomique
Publication of BR112016016524A2 publication Critical patent/BR112016016524A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01019—Phosphoribulokinase (2.7.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01039—Ribulose-bisphosphate carboxylase (4.1.1.39)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

a presente invenção se refere a células de levedura modificadas para expressar uma enzima rubisco do tipo i funcional, e uma fosforibuloquinase de classe ii. a expressão destas enzimas recria um ciclo de calvin nas ditas leveduras com a finalidade de possibilitar que as leveduras usem dióxido de carbono.
BR112016016524-1A 2014-01-16 2015-01-16 célula de levedura transformada BR112016016524A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1450349A FR3016371B1 (fr) 2014-01-16 2014-01-16 Levures modifiees pour utiliser le dioxyde de carbone
FR1450349 2014-01-16
PCT/IB2015/050346 WO2015107496A1 (fr) 2014-01-16 2015-01-16 Levures modifiées pour utiliser le dioxyde de carbone

Publications (1)

Publication Number Publication Date
BR112016016524A2 true BR112016016524A2 (pt) 2018-01-23

Family

ID=50780641

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016016524-1A BR112016016524A2 (pt) 2014-01-16 2015-01-16 célula de levedura transformada

Country Status (10)

Country Link
US (1) US10066234B2 (pt)
EP (1) EP3094717B1 (pt)
JP (1) JP2017502682A (pt)
KR (1) KR20160122735A (pt)
CN (1) CN106103694A (pt)
AU (1) AU2015207758A1 (pt)
BR (1) BR112016016524A2 (pt)
CA (1) CA2937079A1 (pt)
FR (1) FR3016371B1 (pt)
WO (1) WO2015107496A1 (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014129898A2 (en) * 2013-02-22 2014-08-28 Technische Universiteit Delft Recombinant micro-organism for use in method with increased product yield
EP3245285A1 (fr) * 2015-01-16 2017-11-22 Institut National De La Recherche Agronomique Combinaison de chaperonnes bacteriennes affectant positivement la physiologie d'une cellule eucaryote native ou portant une ingenierie
CA3027448A1 (en) 2016-06-14 2017-12-21 Dsm Ip Assets B.V. Recombinant yeast cell
CA3045740A1 (en) 2016-12-23 2018-06-28 Dsm Ip Assets B.V. Improved glycerol free ethanol production
FR3062394B1 (fr) * 2017-01-27 2021-04-16 Enobraq Microorganisme genetiquement optimise pour la production de molecules d'interet
FR3062395B1 (fr) 2017-01-27 2021-04-16 Enobraq Microorganisme genetiquement optimise pour la production de molecules d'interet
CN108624516B (zh) * 2017-03-20 2022-08-26 华东理工大学 一种提高发酵细胞中的代谢产物量及制备idms标准品的方法
CA3072134A1 (en) * 2017-05-31 2018-12-06 Universitat Fur Bodenkultur Wien Yeast expressing a synthetic calvin cycle
BR112019025777A2 (pt) 2017-06-13 2020-06-30 Dsm Ip Assets B.V. célula de levedura recombinante
FR3079525A1 (fr) 2018-03-30 2019-10-04 Enobraq Microorganisme modifie a activite rubisco amelioree
US11692210B2 (en) 2020-09-01 2023-07-04 Braskem S.A. Anaerobic fermentative production of furandicarboxylic acid
CN113201552B (zh) * 2021-04-29 2024-03-22 江南大学 一种分子伴侣质粒系统及其应用
WO2023285280A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
CN117916381A (zh) 2021-07-12 2024-04-19 丹尼斯科美国公司 重组酵母细胞
CN114181879B (zh) * 2021-12-09 2022-11-22 中国科学院深圳先进技术研究院 具有自养能力的底盘细胞及其构建方法和应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080085341A1 (en) * 2006-08-31 2008-04-10 Battelle Memorial Institute Methods and microorganisms for forming fermentation products and fixing carbon dioxide
EP1988168A1 (en) * 2007-05-02 2008-11-05 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Means and methods for providing ribulose bisphosphate-carboxylase-oxygenase with improved properties
US20090191599A1 (en) * 2007-09-10 2009-07-30 Joule Biotechnologies, Inc. Engineered light-harvesting organisms
US8349587B2 (en) * 2011-10-31 2013-01-08 Ginkgo Bioworks, Inc. Methods and systems for chemoautotrophic production of organic compounds

Also Published As

Publication number Publication date
WO2015107496A1 (fr) 2015-07-23
AU2015207758A1 (en) 2016-08-11
FR3016371B1 (fr) 2018-02-02
KR20160122735A (ko) 2016-10-24
US10066234B2 (en) 2018-09-04
JP2017502682A (ja) 2017-01-26
CA2937079A1 (fr) 2015-07-23
EP3094717B1 (fr) 2019-01-16
CN106103694A (zh) 2016-11-09
FR3016371A1 (fr) 2015-07-17
EP3094717A1 (fr) 2016-11-23
US20170002368A1 (en) 2017-01-05

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: suspension of the patent application procedure
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements