FR3062394B1 - Microorganisme genetiquement optimise pour la production de molecules d'interet - Google Patents

Microorganisme genetiquement optimise pour la production de molecules d'interet Download PDF

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Publication number
FR3062394B1
FR3062394B1 FR1750694A FR1750694A FR3062394B1 FR 3062394 B1 FR3062394 B1 FR 3062394B1 FR 1750694 A FR1750694 A FR 1750694A FR 1750694 A FR1750694 A FR 1750694A FR 3062394 B1 FR3062394 B1 FR 3062394B1
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interest
molecules
production
microorganism
genetically modified
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FR3062394A1 (fr
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Cedric Boisart
Nicolas Morin
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EPPEN EUROPE, FR
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Enobraq
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Priority to FR1750694A priority Critical patent/FR3062394B1/fr
Application filed by Enobraq filed Critical Enobraq
Priority to KR1020197024086A priority patent/KR102643557B1/ko
Priority to EP18702647.1A priority patent/EP3574083A1/fr
Priority to JP2019561349A priority patent/JP7240329B2/ja
Priority to US16/480,569 priority patent/US20200277592A1/en
Priority to PCT/EP2018/052005 priority patent/WO2018138289A1/fr
Priority to CN201880021659.1A priority patent/CN110678541A/zh
Priority to BR112019015458-2A priority patent/BR112019015458A2/pt
Publication of FR3062394A1 publication Critical patent/FR3062394A1/fr
Application granted granted Critical
Publication of FR3062394B1 publication Critical patent/FR3062394B1/fr
Priority to US17/573,877 priority patent/US12018300B2/en
Priority to JP2022182031A priority patent/JP2023011947A/ja
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
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    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01044—Phosphogluconate dehydrogenase (decarboxylating) (1.1.1.44)
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01012—Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01019—Phosphoribulokinase (2.7.1.19)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
    • C12Y207/02003—Phosphoglycerate kinase (2.7.2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01031—6-Phosphogluconolactonase (3.1.1.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01039—Ribulose-bisphosphate carboxylase (4.1.1.39)

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  • Chemical & Material Sciences (AREA)
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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un microorganisme génétiquement modifié exprimant une enzyme RuBisCO de type I ou II et une phosphoribulokinase (PRK) fonctionnelles, et dans lequel la voie de glycolyse est au moins partiellement inhibée, ledit microorganisme étant génétiquement modifié de manière à produire une molécule exogène et/ou à surproduire une molécule endogène. Selon l'invention la branche oxydative de la voie des pentoses phosphates peut également être au moins partiellement inhibée. L'invention concerne également l'utilisation d'un tel microorganisme génétiquement modifié, pour la production ou la surproduction d'une molécule d'intérêt et des procédés de synthèse ou bioconversion de molécules d'intérêt.
FR1750694A 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet Active FR3062394B1 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
FR1750694A FR3062394B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet
BR112019015458-2A BR112019015458A2 (pt) 2017-01-27 2018-01-26 Microrganismo geneticamente otimizado para produzir moléculas de interesse
JP2019561349A JP7240329B2 (ja) 2017-01-27 2018-01-26 目的分子を産生するための遺伝的に最適化された微生物
US16/480,569 US20200277592A1 (en) 2017-01-27 2018-01-26 Genetically optimised microorganism for producing molecules of interest
PCT/EP2018/052005 WO2018138289A1 (fr) 2017-01-27 2018-01-26 Microorganisme génétiquement optimisé pour la production de molécules d'intérêt
CN201880021659.1A CN110678541A (zh) 2017-01-27 2018-01-26 用于产生感兴趣的分子的经遗传优化的微生物
KR1020197024086A KR102643557B1 (ko) 2017-01-27 2018-01-26 대상 분자의 생성을 위한 유전자 최적화 미생물
EP18702647.1A EP3574083A1 (fr) 2017-01-27 2018-01-26 Microorganisme génétiquement optimisé pour la production de molécules d'intérêt
US17/573,877 US12018300B2 (en) 2017-01-27 2022-01-12 Genetically optimised microorganism for producing molecules of interest
JP2022182031A JP2023011947A (ja) 2017-01-27 2022-11-14 目的分子を産生するための遺伝的に最適化された微生物

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1750694A FR3062394B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet
FR1750694 2017-01-27

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FR3062394A1 FR3062394A1 (fr) 2018-08-03
FR3062394B1 true FR3062394B1 (fr) 2021-04-16

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US (2) US20200277592A1 (fr)
EP (1) EP3574083A1 (fr)
JP (2) JP7240329B2 (fr)
KR (1) KR102643557B1 (fr)
CN (1) CN110678541A (fr)
BR (1) BR112019015458A2 (fr)
FR (1) FR3062394B1 (fr)
WO (1) WO2018138289A1 (fr)

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PL2958986T3 (pl) * 2013-02-22 2023-01-02 Dsm Ip Assets B.V. Rekombinowany mikroorganizm do zastosowania w sposobie o zwiększonej wydajności produktu
BR112018074490A2 (pt) * 2016-06-14 2019-03-19 Dsm Ip Assets B.V. célula de levedura recombinante
US20220228165A1 (en) * 2019-05-10 2022-07-21 Cncure Biotech Inc. DNA Construct for Diagnosing and Treating Cancer
JP6917434B2 (ja) * 2019-10-25 2021-08-11 長瀬産業株式会社 好気性菌の代謝酵素破壊株およびその培養法
CN112852664B (zh) * 2021-01-14 2022-07-15 西华大学 一种酿酒酵母以及提高酿酒酵母产γ-氨基丁酸的方法
CN112941061B (zh) * 2021-03-19 2021-09-07 中国农业大学 一种Tk-Rubisco突变体、线性组装材料及其制备方法和应用
CN114164237B (zh) * 2021-11-04 2023-03-17 山东阜丰发酵有限公司 一种生产琥珀酸的方法
CN114181879B (zh) * 2021-12-09 2022-11-22 中国科学院深圳先进技术研究院 具有自养能力的底盘细胞及其构建方法和应用
CN115851779B (zh) * 2022-10-29 2024-03-26 昆明理工大学 一种葡萄糖-6-磷酸脱氢酶基因RkZWF1及其应用
CN117210446A (zh) * 2023-03-21 2023-12-12 北京蓝晶微生物科技有限公司 一种重建卡尔文循环提高异养微生物代谢产物产量的方法

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BR9915191A (pt) * 1998-11-10 2001-12-11 Maxygen Inc Métodos para obter um polinucleotìdeo isolado epara produzir uma célula recombinante,protoplasto de célula vegetal, coleção deprotoplastos de célula vegetal, vegetal,polinucleotìdeo, biblioteca, um ou mais membros debiblioteca e célula recombinante alvo
US7826975B2 (en) 2002-07-10 2010-11-02 The Penn State Research Foundation Method for redesign of microbial production systems
US20090191599A1 (en) * 2007-09-10 2009-07-30 Joule Biotechnologies, Inc. Engineered light-harvesting organisms
FR3016371B1 (fr) * 2014-01-16 2018-02-02 Institut National De La Recherche Agronomique Levures modifiees pour utiliser le dioxyde de carbone
EP3145947A2 (fr) * 2014-05-22 2017-03-29 Yeda Research and Development Co., Ltd. Micro-organismes recombinés pouvant fixer le carbone

Also Published As

Publication number Publication date
US20200277592A1 (en) 2020-09-03
JP2020506723A (ja) 2020-03-05
JP2023011947A (ja) 2023-01-24
KR20190115447A (ko) 2019-10-11
BR112019015458A2 (pt) 2020-03-31
FR3062394A1 (fr) 2018-08-03
JP7240329B2 (ja) 2023-03-15
KR102643557B1 (ko) 2024-03-07
EP3574083A1 (fr) 2019-12-04
US20220348897A1 (en) 2022-11-03
US12018300B2 (en) 2024-06-25
WO2018138289A1 (fr) 2018-08-02
CN110678541A (zh) 2020-01-10

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