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  • C07D519/00—Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
  • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
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  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
  • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
  • C07D471/04—Ortho-condensed systems
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  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
  • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
  • C07D487/04—Ortho-condensed systems
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
  • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
  • C07D487/10—Spiro-condensed systems
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  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
  • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
  • C07D491/10—Spiro-condensed systems
  • C07D491/107—Spiro-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring
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  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
  • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
  • C07D495/10—Spiro-condensed systems
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  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
  • C07D498/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
  • C07D498/04—Ortho-condensed systems
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D513/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
  • C07D513/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains two hetero rings
  • C07D513/10—Spiro-condensed systems

Definitions

• the present invention relates to compounds that inhibit multiple mutated forms of KRas, i.e., pan-KRas inhibitors.
• the present invention relates to pan-KRas compounds, pharmaceutical compositions comprising the compounds and methods of use therefor.
• KRas Kirsten Rat Sarcoma 2 Viral Oncogene Homolog
• GDP-bound inactive
• GTP-bound active
• cellular proliferation e.g., see Alamgeer et al., (2013) Current Opin Pharmcol. 13:394-401.
• KRas The role of activated KRas in malignancy was observed over thirty years ago (e.g., see Santos et al., (1984) Science 223:661-664). Aberrant expression of KRas accounts for up to 20% of all cancers and oncogenic KRas mutations that stabilize GTP binding and lead to constitutive activation of KRas.
• KRas mutations at codons 12, 13, 61 and other positions of the KRas primary amino acid sequence are present in 88% of all pancreatic adenocarcinoma patients, 50% of all colon/rectal adenocarcinoma patients, and 32% lung adenocarcinoma patients (e.g., see Prior et all., (2020) Cancer Res 80:2969-74).
• a recent publication also suggested wild type Kras inhibition could be a viable therapeutic strategy to treat KRas WT dependent cancers (e.g., see Bery et al., (2020) Nat. Commun. 11 : 3233).
• KRas The well-known role of KRas in malignancy and the discovery of these frequent mutations in KRas in various tumor types made KRas a highly attractive target of the pharmaceutical industry for cancer therapy. Notwithstanding thirty years of large-scale discovery efforts to develop inhibitors of KRas for treating cancer, no KRas inhibitor has yet demonstrated sufficient safety and/or efficacy to obtain regulatory approval (e.g., see McCormick (2015) Clin Cancer Res. 21 (8):1797-1801 ). [00005] Compounds that inhibit KRas activity are still highly desirable and under investigation, including those that disrupt effectors such as guanine nucleotide exchange factors (e.g., see Sun et al., (2012) Agnew Chem Int Ed Engl.
• pan-KRas inhibitors that demonstrate sufficient efficacy for treating KRas-mediated cancers.
• compounds are provided that inhibit KRas activity.
• the compounds are represented by Formula (I):
• W is: [00009] A is aryl or heteroaryl, wherein the aryl or the heteroaryl is optionally substituted with 1-4 R 1 ;
• [000010] B is selected from:
• Y 1 is hydrogen, L-hydroxy optionally substituted with 1-4 R 8 , L-alkoxy optionally substituted with 1-4 R 8 , halogen, L-C3-C6 cycloalkyl optionally substituted with 1-4 R 9 , L- heteroaryl optionally substituted with 1-4 R 8 , L-aryl optionally substituted with 1-4 R 8 , L-C(O)- NH 2 , and L-heterocycle substituted with 1-2 oxo (-O) or oxo-containing substituent, and optionally further substituted with 1 -2 R 8 ;
• Y 2 is hydrogen or C 1 -C 4 alkyl
• X is selected from: a bond, -S-, -O-, -N ⁇ bound to a fused ring, -CH 2 -, -CH 2 -
• Y 3 is hydrogen or C1-C4 alkyl
• Y 4 is hydrogen or C1-C4 alkyl
• J is selected from: a bond, -O-, -NH-, -CH 2 -, -C(C1-C3 alky 1) 2 -, -CH(C1-C3alkyl)- and -N(C1-C3 alkyl)-;
• each R 4 is independently hydrogen, halogen or Cl — C3 alkyl
• each R 5 is independently hydrogen or C1-C3 alkyl, or two R 5 join to form cycloalkyl or heterocycle;
• each R 6 is independently hydrogen, hydroxy, C1-C4 hydroxyalkyl or heteroaryl,
• each R 7 is independently hydrogen, C1-C3 alkyl, hydroxy, halogen, C1-C3 haloalkyl, -L-NH 2 ,
• two R 7 on adjacent atoms optionally join to form a bond or a fused ring selected from C3-C6 cycloalkyl optionally substituted with 1-4 R 8 , heteroaryl optionally substituted with 1-4 R 8 , aryl optionally substituted with 1-4 R 8 , and heterocycle optionally substituted with 1-4 R 8 , and
• each R 10 is independently hydrogen, halogen, C1-C3 alkyl, or two R 10 join to form cycloalkyl or heterocycle optionally substituted with 1-2 C1-C3 alkyl;
• each L is independently a bond, -C1-C4 alkyl-, -C1-C4 alkyl-NH-, -NH-, -N(C1- C3 alkyl)- or cyclopropyl-CH 2 -;
• compositions comprising a therapeutically effective amount of a compound of the present invention or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable excipient.
• methods for inhibiting the activity of cells containing wild type KRas or one or more KRas mutations comprising contacting the cell with a compound of Formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof as defined herein.
• the contacting is in vitro. In one embodiment, the contacting is in vivo.
• Also provided herein is a method of inhibiting cell proliferation, in vitro or in vivo, the method comprising contacting a cell with an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof as defined herein.
• Also provided are methods for treating cancer in a patient comprising administering a therapeutically effective amount of a compound or pharmaceutical composition of the present invention or a pharmaceutically acceptable salt thereof to a patient in need thereof.
• Also provided herein is a method of treating a KRas wild type, KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H-associated disease or disorder in a patient in need of such treatment, the method comprising administering to the patient a therapeutically effective amount of a compound of Formula (1), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof as defined herein.
• a compound of Formula (I), or a pharmaceutically acceptable salt thereof or a pharmaceutical composition thereof as defined herein for use in the treatment of cancer is also provided herein.
• a compound of Formula (I), or a pharmaceutically acceptable salt thereof for use in the inhibition of KRas wild type or multiple types of KRas mutations for instance KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H mutations.
• Also provided herein is a compound of Formula (I), or a pharmaceutically acceptable salt thereof or a pharmaceutical composition thereof as defined herein, for use in the treatment of a KRas wild type associated disease or disorder or a KRas mutation G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H-associated disease or disorder.
• Also provided herein is a use of a compound of Formula (I), or a pharmaceutically acceptable salt thereof, as defined herein in the manufacture of a medicament for the inhibition of activity of the wild type form of KRas or mutated forms of KRas, including the mutations: G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H.
• Also provided herein is the use of a compound of Formula (1), or a pharmaceutically acceptable salt thereof, as defined herein, in the manufacture of a medicament for the treatment of a KRas wild type associated disease or disorder or a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H-associated disease or disorder.
• Also provided herein is a method for treating cancer in a patient in need thereof, the method comprising (a) determining that the cancer is associated with KRas wild type or a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H mutation (i.e., a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H-associated cancer); and (b) administering to the patient a therapeutically effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof.
• a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H-associated cancer i.e., a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and
• pan-KRas inhibitors including pan- KRas inhibitors such as (R)-1-(7-(8-ethyl-7-fluoro-3-hydroxynaphthalen-1-yl)-8-fluoro-2- (((2R,7aS)-2-fluorohexahydro-.1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3- methylpiperidin-3-ol (Example 5 herein), is for the treatment of cancers that develop resistance following long-term treatment with KRas G12C inhibitors.
• pan- KRas inhibitors such as (R)-1-(7-(8-ethyl-7-fluoro-3-hydroxynaphthalen-1-yl)-8-fluoro-2- (((2R,7aS)-2-fluorohexahydro-.1H-pyrrolizin-7a-yl)methoxy)pyrido
• embodiments of the invention include those wherein a patient suffering from cancer is treated with a herein-described pan-KRas inhibitor such as Example 5 after treatment with a G12C inhibitor becomes ineffective or less effective due to the emergence of resistance-imparting mutations.
• KRas G12C mutant cancers Treatment of KRas G12C mutant cancers with covalent KRas G12C inhibitors such as adagrasib (MRTX849) or sotorasib (AMG510) may result in the incorporation of additional mutations that confer resistance to adagrasib. These mutations could confer resistance through numerous mechanisms.
• covalent KRas G12C inhibitors such as adagrasib (MRTX849) or sotorasib (AMG510) may result in the incorporation of additional mutations that confer resistance to adagrasib. These mutations could confer resistance through numerous mechanisms.
• Mutations that change the mutant cysteine at codon 12 to another amino acid would render the current covalent KRas G12C inhibitors ineffective since current inhibitors make a covalent bond with the mutant cysteine amino acid side chain.
• mutations in the wild type codon 12 glycine to another codon would allow bypass signaling in these tumors through the novel mutant protein.
• the repertoire of codon 12 mutations that can occur with a single nucleotide substitution in the wild type gene (glycine codon) includes mutations commonly observed in cancer such as G12S, G12V, G12R, G12C.
• the repertoire of codon 12 mutations that can occur with single nucleotide base substitutions of the cysteine codon 12 include mutations not frequently observed in cancer, G12Y, G12F and G12W, in addition to G12S and G12R.
• Second-site mutations may also occur in another location in the KRas G 12C mutant gene that confers resistance to KRas G12C inhibitor treatment. These mutations may confer resistance through different mechanisms.
• RAS proteins are small GTPases that normally cycle between an active, GTP-bound state and an inactive, GDP-bound state. RAS proteins are loaded with GTP through guanine nucleotide exchange factors (GEFs; e.g., SOS1) which are activated by upstream receptor tyrosine kinases, triggering subsequent interaction with effector proteins that activate RAS-dependent signaling. RAS proteins hydrolyze GTP to GDP through their intrinsic GTPase activity which is dramatically enhanced by GTPase-activating proteins (GAPs).
• GAPs GTPase-activating proteins
• pan-KRas inhibitors may demonstrate activity against common as well as uncommon codon 12 mutations or mutations that occur in the KRas protein that diminish binding of KRas G12C inhibitors to the KRas protein.
• the present invention relates to inhibitors of KRas wild type and/or multiple mutated forms of KRas, for instance KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61 H mutations.
• the present invention relates to compounds that inhibit the activity of KRas wild type and/or KRas mutations such as G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H, pharmaceutical compositions comprising a therapeutically effective amount of the compounds and methods of use therefor.
• wild type KRas refers to a non-mutant form of a mammalian KRas protein. The assignment of amino acid codon and residue positions for human KRas is based on the amino acid sequence identified by UniProtKB/Swiss-Prot P01 1 16: Variantp.Glyl2Asp.
• a wild type KRas inhibitor refers to compounds of the present invention that are represented by Formula (I), as described herein. These compounds are capable of negatively modulating or inhibiting all or a portion of the enzymatic activity of wild type KRas G12A.
• wild type KRas-associated disease or disorder refers to diseases or disorders associated with or mediated by or having wild type KRas.
• a non-limiting example of a wild type KRas-associated disease or disorder is a wild type KRas-associated cancer.
• KRas G12A refers to a mutant form of a mammalian KRas protein that contains an amino acid substitution of an alanine for a glycine at amino acid position 12.
• the assignment of amino acid codon and residue positions for human KRas is based on the amino acid sequence identified by UniProtKB/Swiss-Prot P01116: Variantp.Glyl2Asp.
• a “KRas G12A inhibitor” refers to compounds of the present invention that are represented by Formula (I), as described herein. These compounds are capable of negatively modulating or inhibiting all or a portion of the enzymatic activity of KRas G12A.
• KRas G12A-associated disease or disorder refers to diseases or disorders associated with or mediated by or having a KRas G12A mutation.
• a non-limiting example of a KRas G12A-associated disease or disorder is a KRas G12A-associated cancer.
• KRas G12C refers to a mutant form of a mammalian KRas protein that contains an amino acid substitution of a cysteine for a glycine at amino acid position 12.
• the assignment of amino acid codon and residue positions for human KRas is based on the amino acid sequence identified by UniProtKB/Swiss-Prot P01116: Variantp.Glyl2Asp.
• a “KRas G12C inhibitor” refers to compounds of the present invention that are represented by Formula (I), as described herein. These compounds are capable of negatively modulating or inhibiting all or a portion of the enzymatic activity of KRas G12C.
• KRas G12C-associated disease or disorder refers to diseases or disorders associated with or mediated by or having a KRas G12C mutation.
• a non-limiting example of a KRas G12C-associated disease or disorder is a KRas G12CD-associated cancer.
• KRas G12D refers to a mutant form of a mammalian KRas protein that contains an amino acid substitution of an aspartic acid for a glycine at amino acid position 12.
• the assignment of amino acid codon and residue positions for human KRas is based on the amino acid sequence identified by UniProtKB/Swiss-Prot P01116: Variantp.Glyl2Asp.
• a “KRas G12D inhibitor” refers to compounds of the present invention that are represented by Formula (I), as described herein. These compounds are capable of negatively modulating or inhibiting all or aportion of the enzymatic activity of KRas G12D.
• KRas G12D-associated disease or disorder refers to diseases or disorders associated with or mediated by or having a KRas G12D mutation.
• a non-limiting example of a KRas G12D- associated disease or disorder is a KRas G12D-associated cancer.
• KRas G12R refers to a mutant form of a mammalian KRas protein that contains an amino acid substitution of an arginine for a glycine at amino acid position 12.
• the assignment of amino acid codon and residue positions for human KRas is based on the amino acid sequence identified by UniProtKB/Swiss-Prot P01116: Variantp.Gly l2Asp.
• a “KRas G12R inhibitor” refers to compounds of the present invention that are represented by Formula (I), as described herein. These compounds are capable of negatively modulating or inhibiting all or a portion of the enzymatic activity of KRas G12R.
• KRas G12R-associated disease or disorder refers to diseases or disorders associated with or mediated by or having a KRas G12R mutation.
• a non-limiting example of a KRas G12R-associated disease or disorder is a KRas G12R-associated cancer.
• KRas G12S refers to a mutant form of a mammalian KRas protein that contains an amino acid substitution of a serine for a glycine at amino acid position 12.
• the assignment of amino acid codon and residue positions for human KRas is based on the amino acid sequence identified by UniProtKB/Swiss-Prot P011.16: Variantp.Glyl 2Asp.
• a “KRas G12S inhibitor” refers to compounds of the present invention that are represented by Formula (1), as described herein. These compounds are capable of negatively modulating or inhibiting all or a portion of the enzymatic activity of KRas G12S.
• KRas G12S-associated disease or disorder refers to diseases or disorders associated with or mediated by or having a KRas G12S mutation.
• a non-limiting example of a KRas G12S-associated disease or disorder is a KRas G12S-associatcd cancer.
• KRas G12V refers to a mutant form of a mammalian KRas protein that contains an amino acid substitution of a valine for a glycine at amino acid position 12.
• the assignment of amino acid codon and residue positions for human KRas is based on the ammo acid sequence identified by UniProtKB/Swiss-Prot P01116: Variantp.Gly 12Asp.
• a “KRas G12V inhibitor” refers to compounds of the present invention that are represented by Formula (I), as described herein. These compounds are capable of negatively modulating or inhibiting all or a portion of the enzymatic activity of KRas G12V.
• KRas Gl 2V-assoc.iated disease or disorder refers to diseases or disorders associated with or mediated by or having a KRas G12V mutation.
• a non-limiting example of a KRas G12V-associated disease or disorder is a KRas G12V-associated cancer.
• KRas G13D refers to a mutant form of a mammalian KRas protein that contains an amino acid substitution of an aspartic acid for a glycine at amino acid position 13.
• the assignment of amino acid codon and residue positions for human KRas is based on the amino acid sequence identified by UniProtKB/Swiss-Prot P01116: Variantp.Glyl2Asp.
• a “KRas G13D inhibitor” refers to compounds of the present invention that are represented by Formula (I), as described herein.
• KRas Gl 3D- associated disease or disorder refers to diseases or disorders associated with or mediated by or having a KRas G13D mutation.
• a non-limiting example of a KRas G13D- associated disease or disorder is a KRas G13D-associated cancer.
• KRas Q61H refers to a mutant form of a mammalian KRas protein that contains an amino acid substitution of a histidine for a glutamine at amino acid position 61.
• the assignment of amino acid codon and residue positions for human KRas is based on the amino acid sequence identified by UniProtKB/Swiss-Prot P01116: Variantp.Glyl2Asp.
• a “KRas Q61H inhibitor” refers to compounds of the present invention that are represented by Formula (I), as described herein. These compounds are capable of negatively modulating or inhibiting all or a portion of the enzymatic activity of KRas Q61H.
• KRas Q61H-associated disease or disorder refers to diseases or disorders associated with or mediated by or having a KRas Q61H mutation.
• a non-limiting example of a KRas Q61H-associated disease or disorder is a KRas Q61H-associated cancer.
• the term “subject,” “individual,” or “patient,” used interchangeably, refers to any animal, including mammals such as mice, rats, other rodents, rabbits, dogs, cats, swine, cattle, sheep, horses, primates, and humans.
• the patient is a human.
• the subject has experienced and/or exhibited at least one symptom of the disease or disorder to be treated and/or prevented.
• the subject has been identified or diagnosed as having a cancer having wild type KRas or a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H mutation (e.g., as determined using a regulatory agency-approved, e.g., FDA-approved, assay or kit).
• the subject has a tumor that is positive for wild type KRas or a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H mutation (e.g., as determined using a regulatory agency- approved assay or kit).
• the subject can be a subject with a tumor(s) that is positive for wild type KRas or a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H mutation (e.g., identified as positive using a regulatory agency-approved, e.g., FDA-approved, assay or kit).
• the subject can be a subject whose tumors have wild type KRas or a KRas G12A, G12C, G12D, G12R, G12S, G12V, G1.3D and/or Q61H mutation (e.g., where the tumor is identified as such using a regulatory agency-approved, e.g., FDA-approved, kit or assay).
• the subject is suspected of having wild type KRas or a KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D or KRas Q61H gene-associated cancer.
• the subject has a clinical record indicating that the subject has a tumor that has wild type KRas or a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H mutation (and optionally the clinical record indicates that the subject should be treated with any of the compositions provided herein).
• an assay is used to determine whether the patient has wild type KRas or a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H mutation using a sample (e.g., a biological sample or a biopsy sample (e.g., a paraffin-embedded biopsy sample) from a patient (e.g., a patient suspected of having wild type KRas-associated or a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H-associated cancer, a patient having one or more symptoms of wild type KRas- associated or a KRas G12A, G12C, G12D, G12R, G12S, G12V, G13D and/or Q61H-associated cancer, and/or a patient that has an increased risk of
• the assays are typically performed, e.g., with at least one labelled nucleic acid probe or at least one labelled antibody or antigen-binding fragment thereof.
• regulatory agency is a country’s agency for the approval of the medical use of pharmaceutical agents with the country.
• a non-limiting example of a regulatory agency is the U.S. Food and Drug Administration (FDA).
• acyl refers to -C(O)CH3.
• C1-C6 alkyl refers to straight and branched chain aliphatic groups having from 1-6 carbon atoms, or 1-4 carbon atoms, or 1-3 carbon atoms, respectively.
• alkyl groups include, without limitation, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, and hexyl.
• C1-C3 haloalky 1 and “C1-C4 haloalkyl” refer to a C1-C3 alkyl chain or C1-C4 alkyl chain, respectively, as defined herein in which one or more hydrogen has been replaced by a halogen. Examples include trifluoromethyl, difluoromethyl and fluoromethyl.
• C1-C4 alkylene group is a C1 -C4 alkyl group, as defined hereinabove, that is positioned between and serves to connect two other chemical groups.
• exemplary alkylene groups include, without limitation, methylene, ethylene, propylene, and butylene.
• C1-C3 alkoxy and “Cl - C4 alkoxy” refer to — OC1 - C3 alkyl and - OC1-C4 alkyl, respectively, wherein the alkyd portion is as defined herein above.
• cycloalkyl as employed herein includes saturated and partially unsaturated cyclic hydrocarbon groups having 3 to 12 carbons, for example 3 to 8 carbons, and as a further example 3 to 6 carbons, wherein the cycloalkyl group additionally is optionally substituted with one or more R 8 or R 9 groups as defined herein.
• cycloalkyl groups include, without limitation, cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptyl, and cyclooctyl.
• cycloalkyl also includes bridged cycloalkyls, such as bicyclo[1.1.1]pentanyl.
• C1-C3 hydro xyalkyl and “C1-C4 hydroxyalkyl” refer to -C1-C3 alkylene-OH and -C1-C4 alkylene-OH, respectively.
• C2-C4 hydroxyalkynyl refers to -C2-C4 alkynylene-
• aryl is a C 6 -C 14 aromatic moiety comprising one to three aromatic rings, which is optionally substituted with one or more R 8 or R 9 groups as defined herein.
• the aryl group is a C 6 -C10 aryl group.
• Examples of aryl groups include, without limitation, phenyl, naphthyl, anthracenyl, fluorenyl, and dihydrobenzofuranyl.
• Aryl also refers to bicyclic or tricyclic ring systems in which one or two rings, respectively, of said aryl ring system may be saturated or partially saturated, and wherein if said ring system includes two saturated rings, said saturated rings may be fused or spirocyclic.
• An example of an aryl ring system comprising two saturated rings wherein the rings are spirocyclic includes the following ring system:
• An "araC1-C6 alkyl” or “arylalkyl” group comprises an aryl group covalently linked to an alkyl group, either of which may independently be optionally substituted or unsubstituted.
• An example of an aralkyl group is (C 6 -C 10 )aryl(Ci- C6)alkyl-, including, without limitation, benzyl, phenethyl, and naphthylmethyl.
• An example of a substituted araC1-C6 alkyl is wherein the alkyl group is substituted with hydroxyalkyl.
• a "heterocyclyl” or “heterocyclic” group is a ring structure having from 3 to 12 atoms, for example 4 to 8 atoms, wherein one or more atoms are selected from the group consisting of N, O, and S wherein the ring N atom may be oxidized to N-O, and the ring S atom may be oxidized to SO or SO2, the remainder of the ring atoms being carbon.
• the heterocyclyl may be a monocyclic, a bicyclic, a spirocyclic or a bridged ring system.
• the heterocyclic group is optionally substituted with one or more R 8 or R 9 groups on ring carbon or ring nitrogen at one or more positions, wherein R 6 is as defined for Formula I.
• the heterocyclic group is also independently optionally substituted on a ring nitrogen atom with alkyl, aralkyl, alkylcarbonyl, or on sulfur with lower alkyl.
• heterocyclic groups include, without limitation, epoxy, azetidinyl, aziridinyl, tetrahydrofuranyl, tetrahydropyranyl, pyrrolidinyl, pyrrolidinonyl, piperidinyl, piperazinyl, imidazolidinyl, imidazopyridinyl, thiazolidinyl, dithianyl, trithianyl, dioxolanyl, oxazolidinyl, oxazolidinonyl, decahydroquinolinyl, piperidonyl, 4-piperidinonyl, quinuclidinyl, thiomorpholinyl, thiomorpholinyl 1,1 dioxide, morpholinyl, azepanyl, oxazepanyl, azabicyclohexanyls, azabicycloheptanyl, azabicyclooctanyls, azabicyclononanyls
• heteroaryl refers to groups having 5 to 14 ring atoms, preferably 5, 6, 9, or 10 ring atoms; having 6, 10, or 14 ⁇ electrons shared in a cyclic array; and having, in addition to carbon atoms, from one to three heteroatoms per ring, or from one to three heteroatoms in at least one ring, selected from the group consisting of N, O, and S.
• heteroaryl groups include acridinyl, azocinyl, benzimidazolyl, benzofuranyl, benzothiofuranyl, benzothiophenyl, benzoxazolyl, benzthiazolyl, benztriazolyl, benztetrazolyl, benzisoxazolyl, benzisothiazolyl, benzimidazolinyl, carbazolyl, 4aH-carbazolyl, carbolinyl, chromanyl, chromenyl, cinnolinyl, 6,7-dihydro-5H-pyrrolo[l,2-a]imidazole, furanyl, furazanyl, imidazolinyl, imidazolyl, 1H-indazolyl, indolenyl, indolinyl, indolizinyl, indolyl, 3H-indolyl, isobenzofuranyl, isochromanyl,
• an effective amount of a compound is an amount that is sufficient to negatively modulate or inhibit the activity of one or more of wild type KRas, KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D or KRas Q61H. Such amount may be administered as a single dosage or may be administered according to a regimen, whereby it is effective.
• a "therapeutically effective amount" of a compound is an amount that is sufficient to ameliorate, or in some manner reduce a symptom or stop or reverse progression of a condition, or negatively modulate or inhibit the activity of one or more of wild type KRas, KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D or KRas Q61H.
• Such amount may be administered as a single dosage or may be administered according to a regimen, whereby it is effective.
• treatment means any manner in which the symptoms or pathology of a condition, disorder or disease are ameliorated or otherwise beneficially altered. Treatment also encompasses any pharmaceutical use of the compositions herein.
• amelioration of the symptoms of a particular disorder by administration of a particular pharmaceutical composition refers to any lessening, whether permanent or temporary, lasting or transient that can be attributed to or associated with administration of the composition.
• A is aryl or heteroaryl, wherein the aryl or the heteroaryl is optionally substituted with 1-4 R 1 ;
• [000089] B is selected from:
• Y 2 is hydrogen or C1-C4 alkyl
• Y 1 and Y 2 join to form: where X is selected from: a bond, -S-, -O-, -N ⁇ bound to a fused ring, -CH 2 -, -CH 2 -N-, -CH 2 -N-CH 2 -, -CH 2 -CH 2 -CH 2 -, -CH 2 -CH 2 -, -O-CH 2 - and -S-CH 2 -;
• Y 3 is hydrogen or Cl -C4 alkyl
• Y 4 is hydrogen or C1-C4 alkyl
• J is selected from: a bond, -O-, -NH-, -CH 2 -, -C(C1-C3 alkyl) 2 -,
• each R 4 is independently hydrogen, halogen or Cl — C3 alkyl
• each R s is independently hydrogen or C1-C3 alkyl, or two R 5 join to form cycloalkyl or heterocycle;
• each R 6 is independently hydrogen, hydroxy, C1-C4 hydroxyalkyl or heteroaryl, [0000102] or two R 6 join to form C3-C6 cycloalkyl or heterocycle;
• each R 7 is independently hydrogen, C1 -C3 alkyl, hydroxy, halogen, C1 -C3 haloalkyl, -L-NH 2 ,
• two R 7 on adjacent atoms optionally join to form a bond or a fused ring selected from C3-C6 cycloalkyl optionally substituted with 1-4 R 8 , heteroaryl optionally substituted with 1-4 R 8 , aryl optionally substituted with 1-4 R 8 , and heterocycle optionally substituted with 1-4 R 8 , and
• each R 10 is independently hydrogen, halogen, C1-C3 alkyl, or two R 10 join to form cycloalkyl or heterocycle optionally substituted with 1-2 C1-C3 alkyl;
• each L is independently a bond, -C1-C4 alkyl-, -C1-C4 alkyl-NH-, -NH-, -N(C1 - C3 alkyl)- or cyclopropyl-CH 2 -; [0000111] each n is 0-3 ;
• Embodiments of the invention also include compounds of Formula (I):
• A is naphthyl optionally substituted with 1-4 R 1 ;
• X is selected from: -CH 2 -, -CH 2 -CH 2 - and -O-CH 2 -;
• each R 4 is independently hydrogen, halogen or Cl - C3 alkyl
• each R 3 is independently hydrogen or C1-C3 alkyl, or two R5 join to form cycloalkyl or heterocycle;
• each R 6 is independently hydrogen, hydroxy, C1-C4 hydroxyalkyl or heteroaryl,
• each R 7 is independently hydrogen, C1-C3 alkyl, hydroxy, halogen, C1-C3 haloalkyl, -L-NH 2 ,
• two R 7 on adjacent atoms optionally join to form a bond or a fused ring selected from C3-C6 cycloalkyl optionally substituted with 1-4 R 8 , heteroaryl optionally substituted with 1-4 R 8 , aryl optionally substituted with 1-4 R 8 , and heterocycle optionally substituted with 1-4 R 8 , and
• each R 10 is independently hydrogen, halogen, C1-C3 alkyl, or two R 10 join to form cycloalkyl or heterocycle optionally substituted with 1-2 C1-C3 alkyl;
• each L is independently a bond, -C1-C4 alkyl-, -C1-C4 alkyl-NH-, -NH-, -N(C1- C3 alkyl)- or cyclopropyl-CH 2 -;
• o is 1-6;
• Embodiments also include such compounds or salts wherein each R 1 is independently selected from halogen, hydroxy, C1-C3 alkoxy and C1-C4 alkyl.
• Embodiments also include such compounds or salts wherein each R 2 , if present, is selected from hydrogen and halogen, and wherein each R 3 , if present, is selected from hydrogen and halogen.
• Embodiments also include such compounds or salts wherein each R 7 is independently selected from hydrogen, C1-C4 alkyl, hydroxy, C1-C3 alkoxy, and wherein two R 7 on non-adjacent atoms optionally join to form a 1-2 carbon bridge.
• Embodiments also include such compounds or salts wherein each R 6 is independently hydrogen or hydroxy.
• Embodiments also include such compounds or salts wherein B is:
• Embodiments also include such compounds or salts wherein B is:
• Embodiments also include such compounds or salts wherein B is: [0000144] Embodiments also include such compounds or salts wherein Y 1 and Y 2 join to form:
• Embodiments also include such compounds or salts wherein Y 1 and Y 2 join to form:
• Embodiments also include such compounds or salts wherein Y 1 and Y 2 join to form:
• Embodiments also include such compounds or salts wherein A is naphthyl.
• Embodiments also include such compounds or salts wherein A is indazolyl.
• Embodiments also include such compounds or salts wherein A is phenyl.
• Embodiments also include such compounds or salts wherein A is pyridyl.
• At least one R 1 is C1-C4 alkyl. [0000152] In certain embodiments of the invention at least one R 1 is halogen, preferably fluorine or chlorine.
• At least one R 1 is hydroxy
• At least one R 2 is C1-C4 alkyl.
• At least one R 2 is halogen, preferably fluorine or chlorine.
• At least one R 2 is hydroxy.
• At least one R 3 is C1-C4 alkyl.
• At least one R 3 is halogen, preferably fluorine or chlorine.
• At least one R 3 is hydroxy.
• R 4 is halogen, preferably fluorine.
• At least one R 5 is C1-C4 alkyl.
• At least one R 5 is hydrogen.
• At least one R 6 is C1-C4 alkyl.
• two R 6 join to form C3-C6 cycloalkyl or heterocycle.
• At least one R 6 is hydrogen.
• both R 6 are C1-C4 alkyl.
• both R 6 are hydrogen.
• Y 1 is L-C3-C6 cycloalkyl, L-heteroaryl, L-aryl, or L- heterocycle. In certain of these embodiments, L is a bond. In certain of these embodiments L is C1-C4 alkyl. In certain of these embodiments L is NH or N(C1-C3) alkyl. [0000169] In certain embodiments Y 1 is L-heteroaryl where the heteroaryl is thietane dioxide, iso-thiazolidine dioxide, imidazopyrazine, pyridine or pyrimidine.
• Y 1 is L-C3-C6 cycloalkyl where the cycloalkyl is preferably cyclobutane, cyclopentane, cyclohexane or cycloheptane.
• Y 1 is L-heterocycle where the heterocycle is preferably pyrrolidinone.
• Y 2 is hydrogen
• Y 2 is C1-C4 alkyl
• At least one R 8 is C1-C4 alkyl, preferably methyl.
• At least one R 8 is hydroxy or C1-C3 alkylhydroxy.
• At least one R 8 is aryl or heteroaryl.
• At least one R 8 is C(O)OH.
• At least one R 8 is -C(O) NH 2 , -C(O)NH(C1-
• R 8 is - NH 2 , -NH(C1-C3 allcyl); -N(C1-C3 alkyl) 2 .
• At least one R 9 is C1-C4 alkyl, preferably methyl.
• At least one R 9 is hydroxy or C1-C3 alkylhydroxy.
• at least one R 9 is aryl or heteroaryl.
• At least one R 9 is C(O)OH.
• At least one R 9 is -C(O)NH 2 , -C(O)NH(C1-
• Y 1 and Y 2 join to form a piperidine, azepane, azocane, thiazepine, diazepane, oxazepane, azetidine, pyrrolidine, piperazine bound to a fused ring via nitrogen or thiomorpholine.
• two R 7 on adjacent atoms optionally join to form a bond or a fused ring selected from C3-C6 cycloalkyl optionally substituted with 1-4 R 8 ; heteroaryl optionally substituted with 1-4 R 8 ; aryl optionally substituted with 1-4 R 8 , and heterocycle optionally substituted with 1-4 R 8 .
• two R 7 on non-adjacent atoms optionally join to form a 1-2 carbon bridge.
• Non-limiting examples of compounds of Formula (I) are selected from the group consisting of the compounds described in the below Examples, and pharmaceutically acceptable salts thereof.
• the compounds of Formula (1) include bis-hydrochloride, trishydrochloride, trifluoroacetic acid, bis-trifluoroacetic acid, and tris-trifluoracetic acid salts of the above compounds.
• the compounds of Formula (1) or pharmaceutically acceptable salt thereof may be formulated into pharmaceutical compositions.
• the invention provides pharmaceutical compositions comprising a wild type KRas, KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H inhibitor according to the invention and a pharmaceutically acceptable carrier, excipient, or diluent.
• Compounds of the invention may be formulated by any method well known in the art and may be prepared for administration by any route, including, without limitation, parenteral, intraperitoneal, intradermal, intracardiac, intraventricular, intracranial, intracerebrospinal, intrasynovial, intrathecal administration, intramuscular injection, intravitreous injection, intravenous injection, intra-arterial injection, oral, buccal, sublingual, transdermal, topical, intranasal, intratracheal, intrarectal, subcutaneous, and topical administration.
• compounds of the invention are administered intravenously in a hospital setting.
• administration may be by the oral route.
• the provided pharmaceutical compositions may be administered to a subject in need of treatment by injection systemically, such as by intravenous injection; or by injection or application to the relevant site, such as by direct injection via syringe, or direct application to the site when the site is exposed in surgery; or by topical administration.
• Parenteral administration can be by bolus injection or continuous infusion.
• Pharmaceutical compositions for injection may be presented in unit dosage form, e.g., in ampoules or in multi-dose containers, with an added preservative.
• compositions can also be formulated as a depot preparation.
• Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection.
• the formulations may be modified with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.
• compositions may, if desired, be presented in a vial, pack or a medical device, including but not limited to a dispenser device which may contain one or more unit dosage forms containing the active ingredient.
• the dispenser device can comprise a syringe having a single dose of the liquid formulation ready for injection.
• the syringe can be accompanied by instructions for administration.
• compositions according to the invention may contain, in addition to the inhibitor, diluents, fillers, salts, buffers, stabilizers, solubilizers, and other materials well known in the art.
• diluents fillers, salts, buffers, stabilizers, solubilizers, and other materials well known in the art.
• the preparation of pharmaceutically acceptable formulations is described in, e.g., Remington's Pharmaceutical Sciences, 18th Edition, ed. A. Gennaro, Mack Publishing Co., Easton, Pa., 1990.
• the term pharmaceutically acceptable salt refers to salts that retain the desired biological activity of the above-identified compounds and exhibit minimal or no undesired toxicological effects.
• examples of such salts include but are not limited to acid addition salts formed with inorganic acids (for example, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, and the like), and salts formed with organic acids such as acetic acid, oxalic acid, tartaric acid, succinic acid, malic acid, ascorbic acid, benzoic acid, tannic acid, pamoic acid, alginic acid, polyglutamic acid, naphthalenesulfonic acid, naphthalenedisulfonic acid, and polygalacturonic acid.
• inorganic acids for example, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, and the like
• organic acids such as acetic acid, oxalic acid, tartaric acid,
• the compounds can also be administered as pharmaceutically acceptable quaternary salts known by those skilled in the art, which specifically include the quaternary ammonium salt of the formula -NR+Z-, wherein R is hydrogen, alkyl, or benzyl, and Z is a counterion, including chloride, bromide, iodide, -O-alkyl, toluenesulfonate, methylsulfonate, sulfonate, phosphate, or carboxylate (such as benzoate, succinate, acetate, glycolate, maleate, malate, citrate, tartrate, ascorbate, benzoate, cinnamoate, mandeloate, benzyloate, and d ipheny lacetate) .
• R is hydrogen, alkyl, or benzyl
• Z is a counterion, including chloride, bromide, iodide, -O-alkyl, toluenesulfonate,
• the active compound is included in the pharmaceutically acceptable carrier or diluent in an amount sufficient to deliver to a patient a therapeutically effective amount without causing serious toxic effects in the patient treated.
• a dose of the active compound for all of the above-mentioned conditions is in the range from about 0.01 to 300 mg/kg, for example 0.1 to 100 mg/kg per day, and as a further example 0.5 to about 25 mg per kilogram body weight of the recipient per day.
• a typical topical dosage will range from 0.01-3% wt/wt in a suitable carrier.
• the effective dosage range of the pharmaceutically acceptable derivatives can be calculated based on the weight of the parent compound to be delivered. If the derivative exhibits activity in itself, the effective dosage can be estimated as above using the weight of the derivative, or by other means known to those skilled in the art.
• the pharmaceutical compositions comprising compounds of the present invention may be used in the methods of use described herein.
• the invention provides for methods for inhibiting wild type KRas, KRas G 12A, KRas G 12C, KRas G 12D, KRas G 12R, KRas G 12S, KRas G 12V and/or KRas Q61H activity in a cell, comprising contacting the cell in which inhibition of wild type KRas, KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V and/or Q61H activity is desired with an effective amount of a compound of Formula (I), pharmaceutically acceptable salts thereof, or pharmaceutical compositions containing the compound or pharmaceutically acceptable salt thereof.
• the contacting is in vitro. In one embodiment, the contacting is in vivo.
• contacting refers to the bringing together of indicated moieties in an in vitro system or an in vivo system.
• "contacting" wild type KRas, KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H with a compound provided herein includes the administration of a compound provided herein to an individual or patient, such as a human, having wild type KRas or a KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H mutation, as well as, for example, introducing a compound provided herein into a sample containing a cellular or purified preparation containing
• a cell in which inhibition of wild type KRas or KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61 H activity is desired is contacted with an effective amount of a compound of Formula (I) or pharmaceutically acceptable salt thereof to negatively modulate the activity of one or more of wild type KRas or KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and KRas Q61H.
• a compound of Formula (I) or pharmaceutically acceptable salt thereof to negatively modulate the activity of one or more of wild type KRas or KRas G12A, KRas G12C, KRas G12D, KRas G12R, KR
• the methods described herein are designed to inhibit undesired cellular proliferation resulting from enhanced wild type KRas or KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and KRas Q61H activity within the cell.
• the cells may be contacted in a single dose or multiple doses in accordance with a particular treatment regimen to affect the desired negative modulation of wild type KRas or KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H.
• KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and KRas Q61H may be monitored in vitro using well known methods, including those described in Examples A and B below.
• the inhibitory activity of exemplary compounds in cells may be monitored, for example, by measuring the inhibition of one or more of wild type KRas or KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H activity of the amount of phosphorylated ERK, for example using the method described in Example C below.
• methods of treating cancer in a patient in need thereof comprising administering to said patient a therapeutically effective amount of a compound of Formula (I) or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising the compound or pharmaceutically acceptable salt thereof are provided.
• compositions and methods provided herein may be used for the treatment of a wild type KRas-associated or KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H-associated cancer in a patient in need thereof, comprising administering to said patient a therapeutically effective amount of a compound of Formula (I), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising the compound or pharmaceutically acceptable salt thereof are provided.
• the wild type KRas-associated or KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H-associated cancer is lung cancer.
• compositions and methods provided herein may be used for the treatment of a wide variety of cancers including tumors such as lung, prostate, breast, brain, skin, cervical carcinomas, testicular carcinomas, etc. More particularly, cancers that may be treated by the compositions and methods of the invention include but are not limited to tumor types such as astrocytic, breast, cervical, colorectal, endometrial, esophageal, gastric, head and neck, hepatocellular, laryngeal, lung, oral, ovarian, prostate and thyroid carcinomas and sarcomas.
• tumor types such as astrocytic, breast, cervical, colorectal, endometrial, esophageal, gastric, head and neck, hepatocellular, laryngeal, lung, oral, ovarian, prostate and thyroid carcinomas and sarcomas.
• these compounds can be used to treat: Cardiac: sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma; Lung: bronchogenic carcinoma (squamous cell, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma; Gastrointestinal: esophagus (squamous cell carcinoma, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma, leiomyosarcoma), pancreas (ductal adenocarcinoma, insulinom
• the concentration and route of administration to the patient will vary depending on the cancer to be treated.
• the compounds, pharmaceutically acceptable salts thereof and pharmaceutical compositions comprising such compounds and salts also may be co-administered with other anti-neoplastic compounds, e.g., chemotherapy, or used in combination with other treatments, such as radiation or surgical intervention, either as an adjuvant prior to surgery or post- operatively.
• Also provided herein is a compound of Formula (I) or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof as defined herein for use in the treatment of cancer.
• Also provided herein is a compound of Formula (I) or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof for use in the inhibition of wild type KRas or KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H.
• a compound of Formula (I) or a pharmaceutically acceptable salt thereof as defined herein in the manufacture of a medicament for the treatment of cancer.
• Also provided herein is a use of a compound of Formula (1) or a pharmaceutically acceptable salt thereof, as defined herein in the manufacture of a medicament for the inhibition of activity of wild type KRas or KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H.
• a compound of Formula (I) or a pharmaceutically acceptable salt thereof, as defined herein in the manufacture of a medicament for the treatment of wild type KRas-associated or a KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H-associated disease or disorder.
• Also provided herein is a method for treating cancer in a patient in need thereof, the method comprising (a) determining that cancer is associated with wild type KRas or a KRas G12A, KRas G12C, KRas G12D, KRas G12R, KRas G12S, KRas G12V, KRas G13D and/or KRas Q61H mutation (e.g., as determined using a regulatory agency-approved, e.g., FDA- approved, assay or kit); and (b) administering to the patient a therapeutically effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof.
• a regulatory agency-approved e.g., FDA- approved, assay or kit
• the compounds of the present invention may be prepared from commercially available reagents using the synthetic methods and reaction schemes described herein, or using other reagents and conventional methods well known to those skilled in the art. For instance, compounds of the present invention may be prepared according to the reaction schemes and examples outlines below.
• the compounds of the present invention may have one or more chiral center and may be synthesized as stereoisomeric mixtures, isomers of identical constitution that differ in the arrangement of their atoms in space.
• the compounds may be used as mixtures or the individual components/isomers may be separated using commercially available reagents and conventional methods for isolation of stereoisomers and enantiomers well-known to those skilled in the art, e.g., using CHIRALPAK® (Sigma- Aldrich) or CHIRALCEL® (Diacel Corp) chiral chromatographic HPLC columns according to the manufacturer’s instructions.
• compounds of the present invention may be synthesized using optically pure, chiral reagents and intermediates to prepare individual isomers or enantiomers. Unless otherwise indicated, all chiral (enantiomeric and diastereomeric) and racemic forms are within the scope of the invention. Unless otherwise indicated, whenever the specification, including the claims, refers to compounds of the invention, the term “compound” is to be understood to encompass all chiral (enantiomeric and diastereomeric) and racemic forms.
• the compounds of the present invention may be in anhydrous, solvated or hydrated forms, and all such forms are included within the scope of the invention.
• Step A methyl 4-(tert-butoxycarbonylamino)-6-chloro-5-fluoro-pyridine-3- carboxylate.
• 4-((tert-butoxycarbonyl)amino)-6-chloro-5-fluoronicotinic acid (14.3 g, 49.2 mmol, 1 equiv.) in MeOH (70 mL) and toluene (210 mL) was added TMSCHN 2 (2 M in hexane, 44.3 mL, 1.8 equiv.) slowly. After stirring at 15 °C for 2 hours, the mixture was quenched with 2N HC1 (100 mL) and layers were separated.
• Step B methyl 4-amino-6-chloro-5-fluoro-pyridine-3-carboxylate.
• MeCN MeCN
• HCbdioxane 4 M, 290 mL, 23.6 equiv.
• Step C methyl 4-amino-6-(8-chloro-1-naphthyl)-5-fluoro-pyridine-3-carboxylate.
• Step D methyl 6-(8-chloro-1-naphthyl)-5-fluoro-4-[(2,2.2- trichloroacety)carbamoylamino] pyridine-3-carboxylate.
• methyl 4-amino-6-(8- chloro-1-naphthyl)-5-fluoro-pyridine-3-carboxylate 5.5 g, 16.6 mmol, 1.0 equiv.
• THF 82 mL
• 2,2,2-trichloroacetyl isocyanate (3.45 g, 18.3 mmol, 2.17 mL, 1.1 equiv.) dropwise.
• Step E 7-(8-chloro-1-naphthyl)-8-fluoro-pyrido[4,3-d]pyrimidine-2.4-diol.
• Step F 2.4-dichloro-7-(8-chloro-1-naphthyl)-8-fluoro-pyrido[4,3-d]pyrimidine.
• Step A 3-methylpiperidin-3-ol: To the solution of tert-butyl 3-hydroxy-3-methyl- piperidine-1-carboxylate (2.45 g, 11.4 mmol) in acetonitrile (9 mL) was added HCEdioxane (4 M, 18 mL) at 0°C, and the mixture was stirred at 0°C for 0.5 h. After completion, the reaction mixture was concentrated to give 3-methylpiperidin-3-ol (1 .75 g, crude) as a yellow oil which was used in the next step without further purification.
• Step B 1-(2,7-dichloro-8-fluoropyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin- 3-ol: To the mixture of 2,4,7-trichloro-8-fluoro-pyrido[4,3-d]pyrimidine (3.65 g, 14.5 mmol), DIEA (7.47 g, 57.8 mmol) in dichloromethane (40 mL) was added 3-methylpiperidin-3-ol (1.75 g, crude) at - 40°C, and the mixture was stirred at - 40°C for 0.5 hour.
• Step A 2A7-trichloro-8-fluoropyrido[4,3-d]pyrimidine: To a mixture of 7-chloro- 8-fluoropyrido[4,3-d]pyrimidine-2,4-diol (2.8 g, 13.0 mmol) in POCh (20 mL) was added D1EA (5.04 g, 39.0 mmol, 6.79 mL) in one portion at 25 °C under N 2 . The mixture was heated to 110 °C and stirred for 2 h. The mixture was concentrated in reduced pressure to give a residue.
• Step B 2,7-dichloro-8-fluoro-4-(piperidin-1-yl)pyrido[4,3-d]pyrimidine: To a mixture of 2,4,7-trichloro-8-fluoro-pyrido[4,3-d]pyrimidine (100 mg, 396 ⁇ mol) and DIPEA (76.8 mg, 594 ⁇ mol, 103 ⁇ L) in DCM (1 mL) was added piperidine (40.5 mg, 475 ⁇ mol, 46.9 ⁇ L) in portions at -40 °C under N 2 . The mixture was stirred at -40 °C for 1 hour. The reaction mixture was filtered and concentrated in vacuum.
• Step A 5-(2-(4-fluorophenyl)acetyl)-2.2-dimethyl- 1,3-dioxane-4.6-dione.
• 2-(4-fluorophenyl)acetic acid 500 g, 3.24 mol, 1 equiv.
• Meldrum's acid 514 g, 3.57 mol, 1.1 equiv.
• DMAP 33.7 g, 275 mmol, 0.085 equiv.
• DIPEA 901 g, 6.97 mol, 1.21 L, 2.15 equiv.
• pivaloyl chloride 430 g, 3.57 mol, 439 mL, 1.1 equiv.
• Step B tert-butyl 4-(4-fluorophenyl)-3-oxobutanoate.
• a solution of 5-(2-(4- fluorophenyl)acetyl)-2,2-dimethyl-1,3-dioxane-4,6-dione (1 kg) in t-BuOH (3 L) was stirred at 90°C for 2 hours, then the mixture solution was concentrated to give the crude solid, and the crude solid was washed with petroleum ether (350 mL) to give tert-butyl 4-(4-fluorophenyl)-3- oxobutanoate (850 g, 94% yield).
• Step C 4-(4-fluorophenyl)-3-oxobutanoic acid.
• a solution of tert-butyl 4-(4- fluorophenyl)-3-oxobutanoate (800 g, 3.17 mol, 1 equiv.) and TFA (2.46 kg, 21.6 mol, 1.6 L, 6.81 equiv.) in DCM (1.6 L) was stirred at 20 °C for 1 hour. The mixture was concentrated to dryness. The residue was washed with petroleum ether (500 mL) to give 4-(4-fluorophenyl)-3-oxobutanoic acid (516 g, 83 % yield).
• Step D 7-fluoronaphthalene-l ,3-diol.
• a solution of 4-(4-fluorophenyl)-3- oxobutanoic acid (450 g, 2.29 mol, 1 equiv.) in CF 3 SO 3 H (8.5 kg, 56 mol, 5 L, 25 equiv.) was stirred at 25 °C for 24 hours, the reaction was cooled to 0°C, and slowly added to ice-water (15 L). Precipitates were formed, and the mixture was filtered to give the crude product. Then the crude was slurred with petroleum ether (1 L), and filtered to give the 7-fluoronaphthalene-1, 3-diol (325 g, 79% yield). Light-yellow Solid.
• Step E 7-fluoro-8-((triisopropylsilyl)ethynyl)naphthalene-1, 3-diol.
• Step F 7-fluoro-3-(methoxymethoxy)-8-((triisonropylsilyl)ethynyl)naphthalen-1- ol.
• Step H ((2-fluoro-6-(metlioxymethoxy)-8-(4,4,5,5-tetramethyl-1.3,2- dioxaborolan-2-yl)naphthalen-1-yl)ethynl) triisopropylsilane.
• Step A 7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1- yl piyalate.
• Step B 8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl piyalate.
• DMF 50 mL
• CsF 9.36 g, 61.6 mmol, 10 equiv.
• Step C 8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl piyalate.
• MeOH MeOH
• Pd/C 200 mg, 10% purity
• Step D 8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-ol.
• MeOH MeOH
• Step E 8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl trifluoromethane sulfonate.
• DIEA 806 mg, 6.24 mmol, 3.0 equiv.
• trifluoromethylsulfonyl trifluorometh anesulfonate 879 mg, 3.12 mmol, 1.5 equiv.
• Step A 7-fluoronaphthalen-1-ol.
• acetic acid 1.50 L
• hydrogen bromide in AcOH (33%, 7.50 mL
• bromine 80.3 g, 503 mmol, 25.9 mL, 1.1 equiv.
• acetic acid 50 mL
• the mixture was stirred at 25 °C for 3 hours.
• the mixture was diluted with DCM (1.5 L), washed with water (3 x 500 mL).
• Step B 7-fluoro-8-(2-triisopropylsilylethynyl)naphthalen-1-ol.
• (bromoethynyl)triisopropylsilane 72.0 g, 275 mmol, 1.20 equiv.
• 7-fluoronaphthalen-1-ol 37.2 g, 230 mmol, 1.0 equiv.
• dichlororuthenium 1-isopropyl-4- methyl-benzene (21.1 g, 34.4 mmol, 0.15 equiv.)
• K 2 CO 3 (31.7 g, 230 mmol, 1.0 equiv.)
• NaOAc 3.77 g, 45.9 mmol, 0.20 equiv.
• Step C [7-fluoro-8-(2-triisopropylsilylethynyl)-1-naphthyl] trifluoromethanesulfonate.
• DCM 600 mL
• DIEA 55.1 g, 426 mmol, 74.2 mL, 2.00 equiv.
• Tf 2 O 90.2 g, 320 mmol, 52.7 mL, 1.50 equiv.
• Step D ((2-fluoro-8-(4,4,5,5-tetramethyl- 1,3,2-dioxaborolan-2-yl)naphthalen-1- yl)ethypyl)triisopropylsilane.
• Step A Ethyl 2-methylene-5-oxotetrahydro-1H-pyrrolizine-7a(5H)-carboxylate.
• ethyl 5-oxopyrrolidine-2-carboxylate (1.50 kg, 9.54 mol, 1.00 equiv.) and 3- chloro-2-(chloromethyl)prop-1-ene (1.91 kg, 15.3 mol, 1.77 L, 1.60 equiv.) in THF (7.50 L) was added LiHMDS (1 M, 19.1 L, 2.00 equiv.) drop-wise at -40 °C under N 2 . The mixture was stirred at 25 °C for 20 hrs.
• Step B ethyl 2,5-dioxotetrahydro-1H-pyrrolizine-7a(5H)-carboxylate.
• DCM dimethyl-5-oxotetrahydro-1H-pyrrolizine-7a(5H)-carboxylate
• MeOH 165 mL
• O3 O3 (15 psi) at -70 °C under N 2 .
• the solution became pale blue, and then the mixture was purged by N 2 for 30 min.
• Step C ethyl 2-hydroxy-5-oxotetrahydro-1H-pyrrolizine-7a(5H)-carboxylate.
• EtOH a solution of ethyl 2,5-dioxotetrahydro-1H-pyrrolizine-7a(5H)-carboxylate (257 g, 1.22 mol, LOO equiv.) in EtOH (1300 mL) was slowly added NaBH4 (13.8 g, 365 mmol, 0.30 equiv.) at 0 °C under N 2 . The mixture was stirred at 0 °C for 10 min.
• Step D ethyl (2S,7aR)-2-fluoro-5-oxotetrahydro-1H-pyrrolizine-7a(5H)- carboxylate.
• DCM 750 mL
• DAST 131 g, 813 mmol, 107 mL, 1.50 equiv.
• Step E ((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a-yl)methanol.
• a suspension of LiA1H4 (33.1 g, 871 mmol, 1.50 equiv.) in THF (625 mL) was added a solution of ethyl (2S,7aR)-2-fluoro-5-oxotetrahydro-1H-pyrrolizine-7a(5H)-carboxylate (125 g, 581 mmol, 1.00 equiv.) in THF (375 mL) drop-wise at 0°C under N 2 .
• the reaction mixture was warmed to 70 °C and stirred at 70 °C for 3 hours.
• the mixture was cooled to 0 °C.
• water 33.0 mL
• NaOH 15%, 99.0 mL
• water 99 mL
• the mixture was stirred at 0 °C stirred for 5 min.
• the mixture was filtered, and the filtered cake was washed with EtOAc (1000 mL x 2).
• the filtrate was dried with MgSO 4 , filtered, and concentrated under reduced pressure to give a residue.
• Step A 2,4.7-trichloro-8-fluoro-pyrido[4.3-d]pyrimidine.
• a mixture of -chloro- 8-fluoro-pyrido[4,3-d]pyrimidine-2,4-diol (20 g, 92.8 mmol, 1 .00 equiv.) in toluene (100 mL) was added POCh (42.7 g, 278 mmol, 25.9 mL, 3.00 equiv.) and N-ethyl-N-isopropylpropan-2-amine (36.0 g, 278 mmol, 48.5 mL, 3.00 equiv.) at 0 °C.
• Step B 2.7-dichloro-8-fluoro-4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine.
• 2,2,2-trifluoroethanol 11.1 g, 111 mmol, 8.01 mL, 1.20 equiv.
• t-BuONa 26.7 g, 278 mmol, 3.00 equiv.
• Step C 7-chloro-8-fluoro-2-((tetrahydro-1H-pyrrolizin-7a(5H)-yl)methoxy)-4- (2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine.
• Step D 7-(8-chloronaphthalen-1-yl)-8-fluoro-2-((tetrahydro-1H-pyrrolizin- 7a(5H)-yl)methoxy) -4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine.
• Step A 2,4,7-trichloro-8-fluoropyrido[4,3-d]pyrimidine.
• 7-chloro- 8-fluoropyrido[4,3-d]pyrimidine-2,4(1H,3H)-dione 100 g, 463 mmol, 1.00 equiv.
• toluene 500 mL
• POCI3 213 g, 1.39 mol, 129 mL, 3.00 equiv.
• DIEA 179 g, 1.39 mol, 242 mL, 3.00 equiv.
• Step B 2.7-dichloro-8-fluoro-4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine.
• 2,4,7-trichloro-8-fluoropyrido[4,3-d]pyrimidine 100 g, 396 mmol, 1.00 equiv.
• 2, 2, 2-trifluoroethanol 59.4 g, 594 mmol, 42.7 mL, 1.50 equiv.
• t-BuONa 152 g, 1.58 mol, 4.00 equiv.
• Step C 7-chloro-8-fluoro-2-((tetrahydro-1H-pyrrolizin-7a(5H)-yl)methoxy)-4- (2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine.
• Step D 8-fluoro-7-( 8-fluoronaphthalen- 1 -y l)-2-((hexahydro- 1 H-pyrrolizin-7a- yl)methoxy)-4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine.
• N-((2-bromo-3,4- dimethylphenyl)carbamothioyl)benzamide (3.56 g, 90% yield) as a light yellow solid;
• Step B 1-(2-bromo-3,4-dimethylphepyl)thiourea: A mixture of N-((2-bromo-3,4- dimethylphenyl)carbamothioyl)benzamide (3.30 g, 9.08 mmol) and NaOH (50 mL, 10% aqueous) was stirred at 80 °C for 3 hours. A white precipitate appeared. After completion, the reaction mixture was cooled to 10 °C. The mixture was filtered and filter cake was washed with water until the pH of the filtrate was 8—9.
• Step C 4-bromo-5 ,6-dimethyIbenzo [ d] thiazol-2-amine : To a solution of 1-(2- bromo-3,4-dimethylphenyl)thiourea (2.20 g, 8.49 mmol) in CHCI3 (30 mL) was added drop-wise Br2 (1.36 g, 8.49 mmol) in CHCI 3 (2 mL) at 0 °C. The mixture was stirred at 70 °C for 1 hour. The reaction mixture was concentrated under reduced pressure to give a residue.
• Step D tert-butyl (4-bromo-5.6-dimethylbenzo[d1thiazol-2-yl) carbamate: A mixture of 4-bromo-5,6-dimethylbenzo[d]thiazol-2-amine (2 g, 7.78 mmol), (Boc) 2 O (2.1 g, 9.62 mmol), DIPEA (3.04 g, 23.5 mmol) and DMAP (96 mg, 786 ⁇ mol) in THF (40 mL) was stirred at 25 °C for 16 hour. Then DIPEA (1.04 g, 8.04 mmol) and (Boc) 2 O (570 mg, 2.61 mmol) was added.
• the mixture was stirred at 25 °C for 4 hours. After completion, the reaction mixture was diluted with water (50 mL) and ethyl acetate (200 mL). The mixture was extracted with ethyl acetate (50 mL x 3). The combined organic layers were washed with brine (20 mL) and dried over anhydrous Na 2 SO 4 . The mixture was filtered and concentrated under reduced pressure to give a residue.
• Step E (2-((tert-butoxycarbonyl)amino)-5.6-dimethylbenzo[d]thiazol-4- yl)boronic acid: A mixture of tert-butyl (4-bromo-5,6-dimethylbenzo[d]thiazol-2-yl)carbamate (1 g, 2.80 mmol), 2-(5,5-dimethyl-1,3,2-dioxaborinan-2-yl)-5,5-dimethyl-1,3,2-dioxaborinane (1.58 g, 7.00 mmol) and AcOK (1.00 g, 10.2 mmol) in dioxane (15 mL) was degassed and purged with N 2 for 3 times.
• Step A 8-((triisopropylsilyl)ethynyl)naphthalen-1-ol: To a solution of naphthalen- 1-ol (500 mg, 3.47 mmol, 1.25 mL), potassium carbonate (479 mg, 3.47 mmol), dichlororuthenium; 1-isopropyl-4-methyl-benzene (531 mg, 867 ⁇ mol) and sodium acetate (56.9 mg, 694 ⁇ mol) in DCE (20.0 mL) was added 2-bromoethynyl(triisopropyl)silane (1.09 g, 4.16 mmol). The reaction was stirred at 40 °C for 12 hours.
• Step B 8-((triisopropylsilyl)ethynyl)naphthalen-1-yl trifluoromethanesulfonate: To a solution of 8-((triisopropylsilyl)ethynyl)naphthalen-1-ol (760 mg, 2.34 mmol) and DIEA (605 mg, 4.68 mmol, 816 ⁇ L) in DCM (8.00 mL) was added Tf 2 O (991 mg, 3.51 mmol, 580 ⁇ L) at -40 °C. The reaction was stirred at 25 °C for 0.5 hour. The reaction was quenched with water (10.0 mL).
• Step C triisopropyl((8-(4,4,5.5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1- yl)ethynyl)silane: To a solution of 8-((triisopropylsilyl)ethynyl)naphthalen-1-yl trifluoromethanesulfonate (950 mg, 2.08 mmol) in dioxane (15.0 mL) were added 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l,3,2-dioxaborolane) (687 mg, 2.70 mmol), Pd(dppf)Ch (152 mg, 208 ⁇ mol) and KO Ac (408 mg, 4.16 mmol).
• Step A 2,4,7-trichloro-8-fluoropyrido[4,3-d]pyrimidine: To a mixture of 7-chloro- 8-fluoropyrido[4,3-d]pyrimidine-2,4-diol (2.8 g, 13.0 mmol) in POCh (20 mL) was added DIEA (5.04 g, 39.0 mmol, 6.79 mL) in one portion at 25 °C under N 2 . The mixture was heated to 110 °C and stirred for 2 hours. After completion, the mixture was concentrated under reduced pressure at 45 °C.
• Step B 1 -(2,7-dichloro-8-fluoropyrido [4, 3-d] pyrimidin-4-yl)-3-methylpiperidin- 3-ol: To a mixture of 2,4,7-trichloro-8-fluoropyrido[4,3-d]pyrimidine (3 g, 11.9 mmol) and DIEA (6.14 g, 47.5 mmol, 8.28 mL) in DCM (10 mL) was added 3-methylpiperidin-3-ol hydrochloride (1.52 g, 13.2 mmol) in portions at -40 °C under N 2 . The mixture was stirred at -40 °C for 1 hour. After completion, the mixture was filtered and concentrated in vacuum.
• Step C 1-(7-chloro-8-fluoro-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol: To a mixture of (hexahydro- 1H-pyrrolizin-7a-yl)methanol (1.43 g, 10.1 mmol) and 1-(2,7-dichloro-8-fluoropyrido[4,3- d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (2.8 g, 8.45 mmol) in dioxane (18 mL) were added DIEA (3.28 g, 25.4 mmol, 4.42 mL) and 4 ⁇ molecular sieves (1.5 g, 8.45 mmol) in one portion at 25 °C under N 2 .
• the mixture was degassed and heated to 78 °C for 9.5 hours under N 2 .
• the reaction mixture was concentrated in vacuum.
• the mixture was diluted with ethyl acetate (500 mL) and filtered.
• the filtrate was diluted with water (100 mL).
• the organic phase was separated.
• the aqueous phase was extracted with ethyl acetate (80 mL).
• the combined organic layers were washed with brine (120 mL) and dried over with anhydrous Na 2 SO 4 .
• the mixture was filtered and concentrated in vacuum to give a residue.
• Step B 2,4-dichloro-7-[8-ethyl-7-fluoro-3-(methoxymethoxy)-1-naphthyl]-8- fluoro-pyrido[4,3-d]pyrimidine: A mixture of POCI 3 (278 mg, 1.81 mmol, 169 ⁇ L) in toluene (3 mL) were added DIEA (141 mg, 1.09 mmol, 190 ⁇ L) and 7-[8-ethyl-7-fluoro-3- (methoxymethoxy)-1-naphthyl]-8-fluoro-pyrido[4,3-d]pyrimidine-2,4-diol (150 mg, 363 ⁇ mol).
• Step A 7-chloro-8-fluoro-2-(((2R.7aS)-2-fluorohexahydro-1H-pyrrolizin-7a- yl)methoxy)-4-(2.2.2-trifluoroethoxy) pyrido[4.3-d]pyrimidine: To a mixture of 2,7-dichloro-8- fluoro-4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine (33.3 g, 1.0 equiv.), D.IEA (54.5 g, 4.0 equiv.) and 4 ⁇ molecular sieves (4.0 g) in THF (340 mL) was added ((2R,7aS)-2-fluorohexahydro- 1H-pyrrolizin-7a-yl)methanol (20.1 g, 1 .2 equiv.).
• Step A 7-(8-ethyl-7-fluoro-3-(methoxyrnethoxy)naphthalen-1-yl)-8-fluoro-2- (((2R.7aS)-2-fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)-4-(2.2.2- trifluoroethoxy)pyrido[4,3-d]pyrimidine: To a mixture of 7-chloro-8-fluoro-2-(((2R,7aS)-2- fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)-4-(2,2,2-trifluoroethoxy)pyrido[4,3- d]pyrimidine (1.0 g, 1.0 equiv.), 2-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)- 4,4,5,5-tetramethyl-1,3,2-d
• Step A (E)-4-(2,3-difluorophepyl)but-3-enoic acid: To a solution of 2,3- difluorobenzaldehyde (100 g, 1.0 equiv.) and 2-carboxyethyl(triphenyl)phosphonium bromide (321 g, 1.1 equiv.) in THF (1 L) was added t-BuOK (1 M in THF, 1.41 L, 2.0 equiv.) at -70°C. The mixture was stirred at -70°C for 1 hour. Then the mixture was warmed up to 20°C and stirred for 1 hour.
• reaction mixture was diluted with water (1 L) and concentrated under reduced pressure to remove the THF. Then the mixture was filtered and the filtrate was adjusted to pH ⁇ 2 with HC1 (IM). The mixture was extracted with ethyl acetate (3 x 1000 mL). The combined organic layers were washed with brine (1000 mL), dried over Na 2 SO 4 , filtered and concentrated under reduced pressure to give the residue.
• Step C 5,6-difluoro-3,4-dihydronaphthalen-1(2H)-one: To a solution of 4-(2,3- difluorophenyl)butanoic acid (13.5 g, 1.0 equiv.) in DCM (300 mL) was added DMF (246 mg, 0.05 equiv.) and oxalyl chloride (17.1 g, 2.0 equiv.). The mixture was stirred at 20 °C for 0.5 hour. The mixture was concentrated under vacuum and then the residue was dissolved in DCM (300 mL). Then, to the mixture was added AICI 3 (12.3 g, 1.5 equiv.).
• Step D 5,6-difluoronaphthalen-1-ol: To a solution of 5,6-difluoro-3,4- dihydronaphthalen-1(2H)-one (11 g, 1.0 equiv.) and HBr (1.48 g, 0.1 equiv.) in AcOH (240 mL) was added a solution of Br 2 (9.65 g, 1.0 equiv.) in AcOH (40 mL) at 0 °C. The mixture was stirred at 25 °C for 1 hour. Then the mixture was diluted with DCM (100 mL) and washed with water (3x100 mL).
• Step F A-(diphenylmethylene)-5,6-difluoronaphthalen-1-amine: A mixture of 5,6- difluoronaphthalen-1-yl trifluoromethanesulfonate (30 g, 1.0 equiv.), diphenylmethanimine (52.2 g, 3.0 equiv.), Pd2(dba)3 (8.80 g, 0.1 equiv.), Xantphos (11.1 g, 0.2 equiv.) and C S2 CO 3 (93.9 g, 3 equiv.) in toluene (500 mL) was degassed and stirred at 90 °C for 12 hours under N 2 atmosphere.
• Step G. 5 6-difluoronaphthal en- 1 -am i ne : A solution of N-(5,6-difluoronaphthalen- 1-yl)- 1,1 -diphenylmethanimine (40 g, 116 mmol, 1.0 equiv.) in HC1MeOH (4 M, 300 mL, 10.3 equiv.) was stirred at 10 °C for 0.5 hour. The mixture was concentrated under vacuum. The pH of the residue was adjusted to ⁇ 8 with saturated aqueous NaHCO 3 solution and extracted with ethyl acetate (3 x 300 mL).
• Step I 5-bromo-6,7-difluoronaphtho[1,2-d][1,2,3]oxadiazole: 2,4-dibromo-5,6- difluoronaphthalen-1-amine (16 g, 47.5 mmol, 1.0 equiv.) was dissolved in AcOH (280 g, 98.2 equiv.) and propionic acid (26.5 g, 7.53 equiv.) and cooled to 0 °C. Then NaNCh (4.91 g, 1.5 equiv.) was added and the mixture was stirred at 20 °C for 1 hour.
• Step J 4-bromo-5,6-difluoronaphthalen-2-ol: To a suspension of 5-bromo-6,7- difluoronaphtho[l,2-d][l,2,3]oxadiazole (11.3 g, 1.0 equiv.) in EtOH (150 mL) and THF (50 mL) at 0 °C was added NaBH4 (3.49 g, 2.33 equiv.). Bubbles evolved immediately. The mixture was stirred at 0 °C for 0.5 hour. The mixture was quenched with water (50 ml) and concentrated under vacuum to remove EtOH. The mixture was extracted with ethyl acetate (3 x 100 mL).
• Step K 8-bromo- 1 ,2-difluoro-6-(methoxymethoxy)naphthalene: To a solution of 4-bromo-5,6-difluoronaphthalen-2-ol (6.3 g, 1 .0 equiv.) in DCM (120 mL) was added D1EA (7.86 g, 2.5 equiv.) and MOMC1 (3.44 g, 1.76 equiv.). The mixture was stirred at 0 °C for 0.5 hour. The reaction mixture was diluted with water (50 mL) and extracted with DCM (3 x 100 mL).
• reaction mixture was diluted with water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layers were washed with brine (50 mL), dried over Na 2 SO 4 , filtered and concentrated under reduced pressure to give a residue.
• Step A 5-(2-(4-fluorophenyl)acetyl)-2,2-dimethyl-1,3-dioxane-4,6-dione: To a mixture of 2-(4-fluorophenyl)acetic acid (250 g, 1 equiv.) and 2, 2-dimethyl-1,3-dioxane-4, 6-dione (257 g, 1.1 equiv.) in ACN (1.25 L) was added DMAP (16.9 g, 0.09 equiv.) at 15 °C. DIPEA (451 g, 2.1 equiv.) was added dropwise below 30 °C for 1 hour.
• Pivaloyl chloride (215 g, 1.1 equiv.) was added dropwise below 40 °C for 1 hour. The mixture was stirred at 45 °C for 3 hours. The mixture was cooled to 0 °C. 4 N aqueous HC1 (5.0 L) was added dropwise to adjust pH to 5 while maintaining the temperature between below 15 °C. The mixture was stirred at 0 °C for 1 hour. The mixture was diluted with H 2 O (15 L) and the pH of the mixture was adjusted to 2 with 4N HC1. The mixture was filtered. The filter cake was washed with H 2 O until the pH of filter cake was 5 ⁇ 6.
• Step B 7-fluoro-l .3-dihydroxy-2-naphthoic acid: 5-(2-(4-fluorophenyl)acetyl)- 2, 2-dimethyl-1,3-dioxane-4, 6-dione (490 g, 1 equiv., crude) was added into CF3SO3H (2.04 kg, 7.8 equiv.) portion wise maintaining the temperature below 30 °C. The mixture was stirred at 20 °C for 2 hour. The mixture was poured into ice water (30 L) slowly. The mixture was filtered.
• Step C 7-fluoronaphthalene-1,3-diol: A mixture of 7-fluoro-1,3-dihydroxy-2- naphthoic acid (375 g, crude, wet) in H 2 O (1.8 L) and ACN (1.8 L) was stirred at 78 °C for 13 hours. The mixture was concentrated to remove ACN. The mixture was diluted with H 2 O (1 L) and saturated NaHCO 3 aqueous (0.3 L), and then extracted with ethyl acetate (4 x 0.5 L).
• Step D 7-fluoro-8-((triisopropylsilyl)ethynyl)naphthalene-1,3-diol: A mixture of 7-fluoronaphthalene-l ,3-diol (173 g, 1 equiv.), (bromoethynyl)triisopropylsilane (266 g, 1.05 equiv.), AcOK (191 g, 2 equiv.) and dichloro(p-cymene)ruthenium(II) dimer (17.8 g, 0.03 equiv.) in dioxane (1.5 L) was degassed and stirred at 100 °C for 3.5 hours.
• the reaction mixture was filtered through a pad of Celite. The filter cake was washed with ethyl acetate (4 x 500 mL). The filtrate was concentrated under reduced pressure to give a residue. The residue was dissolved in ethyl acetate (3 L). The solution was washed with saturated NaHCO 3 aqueous (0.5 L) and brine (0.2 L), dried over anhydrous Na 2 SO 4 , and concentrated to give a residue. The residue was purified by column chromatography [SiO 2 , petroleum ether/ethyl acetate 15.1 to 10:1] to afford a crude product. The crude product was dispersed in n-heptanes (0.5 L) and stirred for 1 hour.
• Step E 7-fluoro-8-((triisopropylsilyl)ethynyl)-3-
• Step F 7-fiuoro-8-((triisopropylsilyl)ethypyl)-3-
• Step G ((6-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5- ((triisopropylsilyl) ethynyl)naphthalen-2-yl) oxy)triisopropylsilane: A mixture of 7-fluoro-8- ((triisopropylsilyl)ethynyl)-3-((triisopropylsilyl)oxy)naphthalen-1-yltrifluoromethanesulfonate (229 g, 1 equiv.), TEA (144 g, 4.0 equiv.), 4,4,5,5-tetramethyl-1,3,2-dioxaborolane (182 g, 4.0 equiv.) and Pd(dppf)Ch (16 g, 0.06 equiv.) in MeCN (1.5 L) was degassed stirred at 78 °C for 4 hours
• Step A 5-ethynyl-6-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2- yl)naphthalen-2-ol: To a solution of ((6-fluoro-4-(4,4,5,5-tetramethyl-l ,3,2-dioxaborolan-2-yl)-5- ((triisopropylsilyl)ethynyl)naphthalen-2-yl)oxy)triisopropylsilane (1.50 kg, 1.0 equiv.) in DMSO (15.0 L) was added CsF (2.19 kg, 6.0 equiv.) in one portion at 25°C under N 2 .
• the reaction mixture was stirred at 25 °C for 12 hrs.
• the mixture was diluted with EtOAc (5.00 L) and water (20.0 L) and the layers were separated.
• the aqueous phase was extracted with EtOAc (5.00 L x 3).
• the combined organic phase was washed with brine (5.00 L x 5), dried with anhydrous Na 2 SO 4 , filtered and concentrated in vacuum.
• the crude product was triturated with n-heptanes (4.5 L) at 50 °C for 6 hrs.
• the mixture was cooled to room temperature and filtered to afford the title compound (600 g, 1.86 mol, 80.1% yield) as a yellow solid.
• Step B 5-ethyl-6-fluoro-4-(4,4,5,5-tetramethyl- 1,3,2-dioxaborolan-2- yl)naphthalen-2-ol: To a solution of 5-ethynyl-6-fluoro-4-(4,4,5,5-tetramethyl-1,3,2- dioxaborolan-2-yl)naphthalen-2-ol (40.0 g, 1.00 equiv.) in THF (400 mL) was added Pd/C (4.00 g, 10.0% purity). The mixture was purged with with H 2 three times and stirred at 25 °C under H 2 (15 Psi) for 5 hrs.
• Step C ((5-ethyl-6-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2- yl)naphthalen-2-yl)oxy)triisopropylsilane: To a solution of 5-ethyl-6-fluoro-4-(4, 4,5,5- tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-2-ol (73.0 g, 1.0 equiv.) in DMF (750 mL) was added imidazole (47.2 g, 3.0 equiv.) and TIPSC1 (89.0 g, 2.0 equiv.).
• Step A 7-( 8-ethylnaphthalen- 1 -yl)-8-fluoro-2-((hexahydro- 1H-pyrrolizin-7a- yl)methoxy)-4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine: A mixture of 7-chloro-8-fluoro-2- ((tetrahydro- 1H-pyrrolizin-7a(5H)-yl)methoxy)-4-(2, 2, 2-trifluoroethoxy)pyrido[4, 3- d]pyrimidine (200 mg, 1.00 equiv.), 2-(8-ethyl-1-naphthyl)-4,4,5,5-tetramethyl-1,3,2- dioxaborolane (201 mg, 1 .50 equiv.), CataCXium A Pd G3 (34.6 mg, 0.10 equiv.), K 3 PO 4 (1 .5 M,
• Step A (2R,4S)-1-tert-butyl 2-methyl 4-((tert-butyldiphepylsilyl)oxy)pyrrolidine- 1.2-dicarboxylate: To a solution of (2R,4S)-1-tert-butyl 2-methyl 4-hydroxypyrrolidine- 1 ,2- dicarboxylate (20.0 g, 1.0 equiv.) and imidazole (11.1 g, 2.0 equiv.) in DCM (200 mL) was added TBDPSC1 (33.6 g, 31.4 mL, 1.50 equiv.). The mixture was stirred at 25 °C for 1 hour.
• Step B (4S)-1-tert-butyl 2-methyl 4-((tert-butyldiphenylsilyl)oxy)-2-(3- chloropropyl)pyrrolidine-1,2-dicarboxylate: To a solution of (2R,4S)-1-tert-butyl 2-methyl 4- ((tert-butyldiphenylsilyl)oxy)pyrrolidine-l ,2-dicarboxylate (37.0 g, 1.0 equiv.) in THF (400 mL) was added LDA (2 M, 49.7 mL, 1.30 equiv.) at -70 °C slowly. The mixture was stirred at -70 °C for 1 hour.
• Step C (4S)-methyl 4-((tert-butyldiphenylsilyl)oxy)-2-(3- chloropropyl)pyrrolidine-2-carboxylate: To a solution of (4S)-1-tert-butyl 2-methyl 4-((tert- butyldiphenylsilyl)oxy)-2-(3-chloropropyl)pyrrolidine-1,2-dicarboxylate (8.60 g, 1.0 equiv.) in MeCN (40 m.L) was added HC1dioxane (4 M, 40 mL, 10.4 equiv.). The mixture was stirred at 20 °C for 0.5 hour. Upon completion, the reaction mixture was concentrated to afford the title compound (7.8 g, crude, HC1 salt) as a yellow solid.
• Step D (2S)-methyl 2-((tert-butyldiphenylsilyl)oxy)hexahydro- 1H-pyrrolizine-7a- carboxylate (90122-E): To a solution of (4S)-methyl 4-((tert-butyldiphenylsilyl)oxy)-2-(3- chloropropyl)pyrrolidine-2-carboxylate (7.80 g, 1.0 equiv., HC1) in ACN (80 mL) was added NaHCO 3 (7.12 g, 3.30 mL) and KI (281 mg). The mixture was stirred at 50 °C for 12 hours.
• Step A benzyl 2-methyl 2-(but-3-en-1-yl)pyrrolidine-1 ,2-dicarboxylate: To a solution of 1 -benzyl 2-methyl (S)-pyrrolidine-1,2-dicarboxylate (220 g, 1 equiv.) in THF (2.2 L) was added LiHMDS (1 M, 1.00 L, 1.2 equiv.) at -65°C. The mixture was stirred at -65°C for 1 hour. Then 4-bromobut- 1-ene (225.61 g, 2 equiv.) was added to the mixture at -65°C.The mixture was stirred at 25 °C for 12 hours.
• Step B benzyl 2-methyl 2-(2-(oxiran-2-yl)ethyl)pyrrolidine-1 ,2-dicarboxylate:. To a solution of benzyl 2-methyl 2-(but-3-en-1-yl)pyrrolidine-1,2-dicarboxylate (242 g, 1 equiv.) in DCM (2.40 L) was added m-CPBA (309 g, 85% purity, 2 equiv.) in portions at 0 °C. The mixture was stirred at 25°C for 5 hours. The mixture was quenched by addition of saturated aqueous Na 2 SO 3 solution (500 mL), extracted with dichloromethane (3 x 500 mL).
• Step C methyl 3-(hydroxymethyl)hexahydro-1H-pyrrolizine-7a-carboxylate: To a suspension of Pd/C (16.0 g, 10% purity) in MeOH (1.0 L) was added benzyl 2-methyl 2-(2-(oxiran- 2-yl)ethyl)pyrrolidine-l ,2-dicarboxylate (130 g, 1 equiv.) under N 2 atmosphere. The suspension was degassed under vacuum and purged with H 2 (50 psi) several times. The mixture was stirred at 25 °C under H 2 (50 psi) for 1 hour.
• Step Fl rac-((3R.7aR)-3-(((tert-butyldiphenylsilyl)oxy)methyl)hexahydro-1H- pyrrolizin-7a-yl)methanol : To a mixture of rac-(3R,7aR)-methyl 3-(((tert- butyldiphenylsilyl)oxy)methyl)hexahydro-1H-pyrrolizine-7a-carboxylate (160 g, 70% purity, 1 equiv.) in THF (2 L) was added LiAIH 4 (12.63 g, 1.3 equiv.) portion wise at -40°C. The mixture was stirred at -40°C for 3 hours.
• LiAIH 4 (12.63 g, 1.3 equiv.
• Step Gl ((3R.7aR)-3-(((tert-butyldiphepylsilyl)oxy)methyl)hexahydro-1H- pyrrolizin-7a-yl)methanol and ((3S.7aS)-3-(((tert-butyldiphenylsilyl)oxy)methyl)hexahydro-1H- pyrrolizin-7a-yl)methanol: The stereoisomers of rac-((3R,7aR)-3-(((tert- butyldiphenylsilyl)oxy)methyl)hexahydro-1H-pyrrolizin-7a-yl)methanol (175 g) were separated by SFC (column: Phenomenex-Cellulose-2 (250mm x 50mm, 10um); mobile phase: [0.1%NH3- H2O IPA]; B%: 40%-40%, 4.9min) to afford title compounds ((3R,7aR)-3-((
• Step F2 rac-((3S,7aR)-3-(((tert-butyldiphenylsilyl)oxy)methyl)hexahydro-1H- pyrrolizin-7a-yl)methanol: To a solution of methyl 3-[[tert-butyl(diphenyl)silyl]oxymethyl]- l ,2,3,5,6,7-hcxahydropyrrolizine-8-carboxylate (83.0 g, 1 equiv.) in THF (830 mL) was added LAH (8.05 g, 1.3 equiv.) portion wise at -40°C. The mixture was stirred at -40°C for 2 hours.
• Step G2 ((3S,7aR)-3-(((tert-butyldiphepylsilyl)oxy)methyl)hexahydro-1H- pyrrolizin-7a-yl)methanol and ((3R,7aS)-3-(((tert-butyldiphepylsilyl)oxy)methyl)tetrahydro-1H- pyrrolizin-7a(5H)-yl)methanol: The stereoisomers of rac-((3S,7aR)-3-(((tert- butyldiphenylsilyl)oxy)methyl)hexahydro-1H-pyrrolizin-7a-yl)methanol (110g) were separated by SFC (column: REGIS (s,s) WHELK-01 (250 mm x 50 mm, 10 um); mobile phase: [0.1%NH 3 - H 2 O ETOH];B%: 40%-40%, 3.7min) to afford title compounds
• Step A (2S,4R)-1-tert-butyl 2-methyl 4-((tert-butyldiphenylsilyl)oxy)pyrrolidine- 1 ,2-dicarboxylate: To a solution of (2S,4R)-1-tert-butyl 2-methyl 4-hydroxypyrrolidine-1,2- dicarboxylate (20.0 g, 1.0 equiv.) in DCM (250 mL) was added imidazole (11.1 g, 2.0 equiv.) and TBDPSC1 (26.9 g, 1.20 equiv.) at 0 °C. The mixture was stirred at 25 °C for 1 hour.
• Step B (4R)-1-tert-butyl 2-methyl 4-((tert-butyldiphenylsilyl)oxy)-2-(3- chloropropyl) pyrrolidine-1,2-dicarboxylate: To a solution of (2S,4R)-1-tert-butyl 2-methyl 4- ((tert-butyldiphenylsilyl)oxy)pyrrolidine-1,2-dicarboxylate (36.0 g, 1.0 equiv.) in THF (200 mL) was added LDA (2.0 M in THF, 48.4 mL, 1 .30 equiv.). The mixture was stirred at -70 °C for 1 hour.
• Step C (4R)-methyl 4-((tert-butyldiphenylsilyl)oxy)-2-(3- chloropropyl)pyrrolidine-2-carboxylate: To a solution of (4R)-1-tert-butyl 2-methyl 4-((tert- butyldiphenylsilyl)oxy)-2-(3-chloropropyl)pyrrolidine-1,2-dicarboxylate (36.0 g, 1.0 equiv.) in ACN (200 mL) was added HC1dioxane (4.0 M, 200 mL) at 0 °C. The mixture was stirred at 0 °C for 1 hour. The mixture was concentrated under reduced pressure to afford the title compound (34.0 g, crude, HC1) as a yellow solid.
• Step D (2R,7aS)-methyl 2-((tert-butyldiphenylsilyl)oxy)hexahydro-1H- pyrrolizine-7a-carboxylate _ (peak _ A) _ and _ (2R,7aR)-methyl2-((tert- butyldiphepylsilyl)oxy)hexahydro- 1H-pyrrolizine-7a-carboxylate (peak B): To a solution of (4R)- methyl 4-((tert-butyldiphenylsilyl)oxy)-2-(3-chloropropyl)pyrrolidine-2- carboxylate (34.0 g, 1.0 equiv, HC1) in ACN (300 mL) was added NaHCOj (28.8 g, 5.0 equiv.) and KI (1.14 g, 0.1 equiv.).
• Step E ((2R,7aS)-2-((tert-butyldiphenylsilyl)oxy)hexahydro-1H-pyrrolizin-7a- yl)methanol: To a solution of (2R,7aS)-methyl 2-((tert-butyldiphenylsilyl)oxy)hexahydro-1H- pyrrolizine-7a- carboxylate (4.50 g, 1.0 equiv.) in THF (100 mL) was added L1A1H4 (1.21 g, 3.0 equiv.) at -40 °C. The mixture was stirred at -40 °C for 1 hour.
• Step A 5-ethyl-6-fluoro-4-(8-fluoro-2-(((2R,7aS)-2-fluorohexahydro-1H- pyrrolizin-7a-yl)methoxy)-4-(2,2.2-trifluoroethoxy)pyrido[4,3-d]pyrimidin-7-yl)naphthalen-2-ol: To a solution of 7-chloro-8-fluoro-2-(((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a- yl)methoxy)-4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine (28.4 g, 1.0 equiv.), 5-ethyl-6- fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-2-ol (25.6 g,
• the reaction was stirred at 100 °C for 3 hours under N 2 .
• the mixture was diluted with water (300 mL) and extracted with ethyl acetate (3 x 200 mL). The combined organic layers were dried over anhydrous sodium sulfate, concentrated.
• Step A tert-butyl 2,4-dioxo-1,3,7-triazaspiro[4.61undecane-7-carboxylate: To a solution of tert-butyl 3 -oxoazepane- 1 -carboxylate (2.00 g, 1.0 equiv.) and (NH4) 2 CO 3 (2.70 g, 3.0 equiv.) in EtOH (10 mL) and H 2 O (10 mL) was added KCN (1.12 g, 1.83 equiv.). The reaction was stirred at 85 °C for 16 hours.
• Step A tert-butyl 2-(hydroxymethyl)-7.8-dihydro-4H-pyrazolo[1,5- a] [1 ,4]diazepine-5(6H)-carboxylate: To a solution of 5-(tert-butoxycarbonyl)-5,6,7,8-tetrahydro- 4H-pyrazolo[l,5-a][l,4]diazepine-2-carboxylic acid (500 mg, 1.0 equiv.) in THF (10 mL) was added LiA1H4 (135 mg, 2.0 equiv.) at 0 °C.
• Step B 5,6,7,8-tetrahydro-4H-pyrazolo[1,5-a][L4]diazepin-2-ylmethanol: To the solution of tert-butyl 2-(hydroxymethyl)-7,8-dihydro-4H-pyrazolo[l,5-a][l,4]diazepine-5(6H)- carboxylate (260 mg, 1 .0 equiv.) in ACN (4 mL) was added HC1dioxane (4 M, 2.43 mL, 10 equiv.) at 0 °C. The reaction was stirred at 0 °C for 0.5 hour. The reaction mixture was concentrated to give a residue.
• Step A benzyl 3-( sulfamoylamino)piperidine- 1 -carboxylate: To a solution of benzyl 3-aminopiperidine-1-carboxylate (500 mg, 1.0 equiv.) in dioxane (10 mL) was added sulfamide (410 mg, 254 ⁇ L, 2.0 equiv.). After stirring at 80 °C for 12 hours, another portion of sulfamide (615 mg, 382 ⁇ L, 3.0 equiv.) was added. The mixture was stirred at 80 °C for 5 hours.
• Step B 3-(sulfamoylamino)piperidine: To a solution of benzyl 3- (sulfamoylamino)piperidine-1-carboxylate (420 mg, 1.0 equiv.) in methanol (10 mL) was added Pd/C (0.1 g, 10% purity) under N 2 atmosphere. The suspension was degassed and purged with H 2 3 times. The mixture was stirred under H 2 (15 Psi) at 20 °C for 12 hours.
• Pd/C 0.1 g, 10% purity
• Step A tert-butyl-2-(pyrrolidine-1-carbonyl)-7.8-dihydro-4H-pyrazolo[L5- ⁇ ] [ 1 ,4]diazepine-5(6H)-carboxylate: To a solution of 5-tert-butoxycarbonyl-4, 6,7,8- tetrahydropyrazolo[l,5-a][l,4]diazepine-2-carboxylic acid (160 mg, 1.0 equiv.), pyrrolidine (121 mg, 3.0 equiv.) and triethylamine (74.8 mg, 103 ⁇ L, 1.3 equiv.) in DMF (1.5 mL) was added HATU (281 mg, 1 .3 equiv.) at 0°C.
• Step A methyl 1 -(chlorocarbonyl)cyclopropanecarboxylate: To a mixture of 1- (methoxycarbonyl)cyclopropanecarboxylic acid (2.0 g, 1.0 equiv.), DMF (101 mg, 0.1 equiv.) in DCM (15 mL) was added (COCl)s (2.64 g, 1.82 mL, 1.5 equiv.). The mixture was stirred at 0-20 °C for 1 hour. The mixture was concentrated and purified by column chromatography (Silica gel, Petroleum ether/Ethyl acetate 5 : 1 to 1 : 1) to afford the title compound (2.2 g, 97% yield) as a yellow oil.
• Step B methyl 1 -(4-methylpiperazine-1-carbonyl)cyclonropanecarboxylate: To a mixture of methyl 1 -chlorocarbonylcyclopropanecarboxylate (2.2 g, 1.0 equiv.), TEA (4.11 g, 3.0 equiv.) in DCM (15 mL) was added 1 -methylpiperazine (1.63 g, 1.2 equiv.). The mixture was stirred at 0-20 °C for 1 hour.
• Step C (1-((4-methylpiperazin-1-yl)methyl)cyclopropyl)methanol: To a solution of methyl 1-(4-methylpiperazine-1-carbonyl)cyclopropanecarboxylate (1.5 g, 1.0 equiv.) in THF (40 mL) was added LiAIH 4 (503 mg, 2.0 equiv.) portion wise under N 2 . The suspension was degassed and purged with Ns for 3 times. The mixture was stirred at 0-15 °C for 2 hours. The mixture was quenched with water (500 ⁇ L), 15% NaOH aqueous (500 ⁇ L), water (1.5 mL), and filtered.
• LiAIH 4 503 mg, 2.0 equiv.
• Step A tert-butyl ( (2-oxoimidazol idin-4-yl)methyl)carbamate: A mixture of tertbutyl N-[(2,5-dioxoimidazolidin-4-yl)methyl]carbamate (100 mg, 1.0 equiv.) and BH3*Me2S (10.0 M, 2.0 equiv.) in THF (3 mL) at 0 °C was degassed and stirred at 60°C for 1 hour under N 2 atmosphere. The reaction mixture was quenched by addition of MeOH (10 mL) at 0°C. Then it was extracted with ethyl acetate (3 x 20 mL).
• Step B 4-(aminomethyl)imidazolidin-2-one: To a solution of tert-butyl N-[(2- oxoimidazolidin-4-yl)methyl]carbamate (60.0 mg, 1.0 equiv.) in MeOH (2 mL) was added HC1 (1 M, 5.0 equiv.). The mixture was stirred at 25 °C for 2 hours. The reaction mixture was concentrated under reduced pressure to afford the title compound (50.0 mg, crude) as a white solid. The crude product was used for the next step directly.
• Step A benzyl (2-(2,5-dioxoimidazolidin-4-yl)ethyl)carbamate: Ammonium carbonate (25.5 g, 11.0 equiv.) was added to benzyl N-(3-oxopropyl)carbamate (5.00 g, 1.00 equiv.) in methanol (40.0 mL) and water (36.0 mL) under a flow nitrogen gas. To the reaction mixture was added potassium cyanide (1.97 g, 1.25 equiv.) and the resulting was stirred at 20 °C for 12 hours.
• Step B benzyl (2-(2-oxoimidazolidin-4-yl)ethyl)carbamate: To a solution of benzyl N-[2-(2,5-dioxoimidazolidin-4-yl)ethyl]carbamate (1.00 g, 1.0 equiv.) in tetrahydrofuran (15.0 mL) was added borane dimethyl sulfide complex (10 M, 1.80 mL, 5.0 equiv.) at 0 °C. The mixture was stirred at 50 °C for 1.5 hours. The mixture was quenched with methanol (10.0 mL). The mixture was concentrated in vacuum to produce a residue.
• the residue was purified by prep- HPLC (column: Welch Ultimate XB-CN 250 x 50 x 10um; mobile phase: [Hexane-EtOH]; B%: 10%-35%, 12min).
• the desired fraction was collected and lyophilized to give a residue.
• the residue was further re-purified by column chromatography on silica gel (ethyl acetate) to afford the title compound (150 mg, 13.9% yield) as a white solid.
• Step C 4-(2-aminoethyl)imidazolidin-2-one: To a solution of benzyl JV-[2-(2- oxoimidazolidin-4-yl)ethyl]carbamate (80.0 mg, 1.00 equiv.) in tetrahydrofuran (5.00 mL) was added palladium/carbon (20 mg, 10% purity) under nitrogen atmosphere. The suspension was degassed and purged with hydrogen for 3 times. The mixture was stirred under hydrogen (15 psi) at 20 °C for 2 hours. The mixture was filtered and the filter cake was washed with methanol (10.0 mL). The filtrate was concentrated to afford the title compound (40 mg, crude) as a white solid.
• Step A 4-((benzyl(methyl)amino)methyl)azetidin-2-one: To a solution of 4- (iodomethyl)azetidin-2-one (100 mg, 1.0 equiv.) and A-methyl-1-phenyl-methanamine (86.2 mg, 1.5 equiv.) in acetonitrile (1.5 mL) was added potassium carbonate (196 mg, 3.0 equiv.). The mixture was stirred at 60 °C for 2 hours. After completion, the reaction mixture was cooled to 25 °C and filtered. Then the filtrate was partitioned between ethyl acetate (10 mL) and water (10 ml).
• Step B 4-((methylamino)methyl)azetidin-2-one: A mixture of 4- [[benzyl(methyl)amino]methyl]azetidin-2-one (60.0 mg, 1.0 equiv.) and Pd/C (20.0 mg, 10% purity) in methyl alcohol (0.5 mL) was degassed and purged with hydrogen for 3 times, and then the mixture was stirred at 25 °C for 2 hours under hydrogen atmosphere (15 psi). The reaction mixture was concentrated under reduced pressure to afford the title compound (13.0 mg, 39% yield) as yellow liquid; The crude product was used for the next step directly.
• Step A methyl 5-(((tert-butoxycarbopyl)amino)methyl)-1-methyl-1H-pyrazole-3- carboxylate: A mixture of methyl 5-bromo-1-methyl-1H-pyrazole-3-carboxylate (900 mg, 1 equiv.), Potassium [[(tert-Butoxycarbonyl)amino]methyl]trifluoroborate (1.46 g, 1.5 equiv.), Pd(OAc) 2 (73.8 mg, 0.08 equiv.) and XPhos (313 mg, 0.16 equiv.) and C S2 CO 3 (4.02 g, 3 equiv.) in THF (50 mL) and water (5 mL) was degassed and stirred at 100 °C for 2 hours under N 2 atmosphere.
• Step B tert-butyl ((3-carbamoyl-1-methyl-1H-pyrazol-5-yl)methyl)carbamate: A mixture of methyl 5-(((tert-butoxycarbonyl)amino)methyl)-1-methyl- 1H-pyrazole-3-carboxylate (400 mg, 1 equiv.) in NH 3 .H 2 O (3.64 g, 4 mL, 25% NH3, 17.48 equiv.) was stirred at 20 °C for 2 hours under N 2 atmosphere. The reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (20 mL x 3). The combined organic layers were washed with brine (10 mL x 1), dried over anhydrous sodium sulfate, filtered and concentrated to give the title compound (220 mg, 58% yield) as a white solid.
• Step C 5-( aminomethyl)- 1 -methyl- 1 H-pyrazole-3 -carboxamide : To a mixture of tert-butyl ((3-carbamoyl-1-methyl-1H-pyrazol-5-yl)methyl)carbamate (100 mg, 1 equiv.) in CH 2 CI 2 (0.5 mL) was added TFA (770 mg, 0.5 mL, 17.2 equiv.) at 0 °C, the mixture was stirred at 25 °C for 12 hours. The reaction mixture was concentrated under reduced pressure to give the title compound (170 mg, crude, TFA) as a yellow oil.
• Step A 5-ethynyl-6-fluoro-4-(8-fluoro-2-(((2R.7aS)-2-fluorohexahydro-1H- pyrrolizin-7a-yl)methoxy)-4-(2,2.2-trifluoroethoxy)pyrido[4,3-d]pyrimidin-7-yl)naphthalen-2-ol: To a solution of 8-fluoro-7-(7-fluoro-8-((triisopropylsilyl)ethynyl)-3- ((triisopropylsilyl)oxy)naphthalen-1-yl)-2-(((2R,7aS)-2-fiuorohexahydro-1H-pyrrolizin-7a- yl)methoxy)-4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine (1.50 g, 1 equiv.)
• Step A 5-chloro-6-fluoro-1 ,4-dihydro-1.4-epoxynaphthalene: To a solution 1- bromo-3-chloro-2,4-difluorobenzene (250 g, 1 equiv.) and furan (150 g, 2 equiv.) in toluene (2.5 L) was added n-BuLi (2.5 M, 1.2 equiv.) dropwise over 0.5 hour at -15 °C. The reaction mixture was stirred at 20 °C for 12 hours. After reaction completion, the mixture was quenched with water (2 L) and filtered. The filtrate was separated.
• Step B 8-chloro-7-fluoronaphthalen-1-yl piyalate: A reaction mixture of 5-chloro- 6-fluoro-1,4-dihydro-1,4-epoxynaphthalene (162 g, 1 equiv.) in concentrated HC1 (1.02 kg, 12.2 equiv.) and EtOH (1 .2 L) was heated to 80 °C for 6 hours. The reaction mixture was concentrated in vacuum. The pH of the residue was adjusted to 7 with saturated aqueous NaHCO 3 and extracted with ethyl acetate (2 x 2 L). The combined organic layers were dried over Na 2 SO 4 , filtered and concentrated.
• Step C. 8-chloro-7-fluoronaphthalen-1-yl piyalate A mixture of 8-chloro-7- fluoronaphthalen-1-ol (124 g, 1 equiv.) and DIEA (489 g, 6 equiv.), 4 ⁇ molecular sieves (120 g) in dichloromethane (1 .5 L) was stirred for 10 minutes at 20 °C. Then PivCl (231 g, 1.3 equiv.) was added to the mixture dropwise at -40 °C. The reaction mixture was stirred at -40 °C for 20 minutes. The reaction mixture was quenched with water (1 L) and the layers were separated.
• Step D 8-chloro-7-fluoro-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2- yl)naphthalen-1-yl piyalate: A mixture of 8-chloro-7-fluoronaphthalen-1-yl pivalate (8.00 g, 1.0 equiv.), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l,3,2-dioxaborolane) (7.24 g, 1.0 equiv.), (1,5- Cyclooctadiene)(methoxy)iridium(I) dimer (944 mg, 0.05 equiv.) and 4-tert-butyl-2-(4-tert-butyl- 2-pyridyl)pyridine (918 mg, 0.12 equiv.) in n-hexane (220 mL) was degassed and stirred at 65 °C
• Step E 8-chloro-7-fluoro-3-hydroxynaphthalen-1-yl piyalate: To a solution of 8- chloro-7-fluoro-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl pivalate (50.0 g, 1 .0 equiv.) and H 2 O2 (116 g, 98.3 mL, 30% purity, 8.3 equiv.) in THF (300 mL) was added AcOH (502 g, 478 mL, 68 equiv.) at 0 °C. The solution was stirred at 20 °C for 2 hours.
• Step F 8-chloro-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl piyalate: To a solution of 8-chloro-7-fluoro-3-hydroxynaphthalen-1-yl pivalate (5.4 g, 1.0 equiv.) in DCM (55 mL) were added DIPEA (7.06 g, 9.51 mL, 3 equiv.) and chloro(methoxy)methane (2.72 g, 2.57 mL, 1.8 equiv.) dropwise at 0 °C. The mixture was stirred at 0 °C for 1.5 hours.
• Step G 8-chloro-7-fluoro-3-(methoxymethoxy)naphthalen- 1 -ol : To a solution of 8-chloro-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl pivalate (8.00 g, 1.0 equiv.) in MeOH (120 mL) was added KOH (5.38 g, 1.0 equiv.) at 0 °C, The mixture was stirred at 20 °C for 1 hour.
• 8-chloro-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl trifluoromethanesulfonate To a solution of 8-chloro-7-fluoro-3-(methoxymethoxy)naphthalen-1- ol (5.00 g, 1.0 equiv.) in DCM (60 mL) were added DIEA (2.52 g, 3.39 mL, 1.0 equiv.) and trifluoromethanesulfonic anhydride (8.24 g, 4.82 mL, 1.5 equiv.) dropwise at -40 °C. The mixture was stirred at -40 °C for 1 .5 hours.
• Step 1 (8-chloro-7-fluoro-3-(methoxymethoxy)naphthalen-1- yl)trimethylstannane: To the mixture of [8-chloro-7-fluoro-3-(methoxymethoxy)-1-naphthyl] trifluoromethanesulfonate (2 g, 1 equiv.), trimetliyl(trimethylstannyl)stannane (5.40 g, 3.2 equiv.), LiCl (654.37 mg, 3.0 equiv.) in toluene (80 mL) was added Pd(PPh3)4 (595 mg, 0.1 equiv.) under N 2 .
• Step J 7-(8-chloro-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro-2- (((2R,7aS)-2-fluorotetrahydro-1H-pyrrolizin-7a(5H)-yl)methoxy)-4-(2,2,2- trifluoroethoxy)pyrido[4.3-d]pyrimidine: To a to a solution of 7-chloro-8-fluoro-2-(((2R,7aS)-2- fluorohexahydro-1 H-pyrrolizin-7a-yl)methoxy)-4-(2,2,2-trifluoroethoxy)pyrido[4,3- d]pyrimidine (625 mg, 1 equiv.) and [8-chloro-7-fluoro-3-(methoxymethoxy)-1-naphthyl]- trimethyl-stannane (1.72 g,
• Step A 5-(2-(3-bromo-4-fluorophenyl)acetyl)-2,2-dimethyl-1.3-dioxane-4,6- dione: To a mixture of 2-(3-bromo-4-fluoro-phenyl)acetic acid (330 g, 210 mL, 1.0 equiv.) and 2, 2-dimethyl-1,3-dioxane-4, 6-dione (225 g, 1.1 equiv.) in MeCN (1.65 L) was added DMAP (14.7 g, 0.085 equiv.) in one portion at 15 °C under N 2 atm.
• Step B 8-bromo-7-fluoro-1.3-dihydroxy-2-naphthoic acid: 5-[2-(3-bromo-4- fluoro-phenyl)acetyl]-2,2-dimethyl-l ,3-dioxane-4, 6-dione (600 g, 1.0 equiv.) was slowly added to CF3SO3H (1.30 L) in four portions maintaining the temperature at 25 °C - 50 °C for 1 hour with ice-water cooled bath. Then the mixture was stirred at 20 °C for 2 hours and water (10 L) was added slowly to the reaction mixture. The mixture was filtered. The filter cake was collected and concentrated to afford the title compound (1200 g, crude) as a yellow solid.
• Step C. 8-bromo-7-fluoronaphthalene- 1 ,3-diol A solution of 8-bromo-7-fluoro- 1 ,3-dihydroxy-naphthalene-2-carboxylic acid (1.3 kg, 1.0 equiv.) in water (700 mL) and ACN (700 mL) was stirred at 85 °C for 12 hours. The mixture was concentrated and the residue was diluted with water (1 L), extracted with ethyl acetate (2 L x 2).
• Step D 8-bromo-7-fluoro-3-((triisopropylsilyl)oxy)naphthalen-1-ol: To a solution of 8-bromo-7-fluoro-naphthalene-l ,3-diol (10.0 g, 1.0 equiv.) and DIEA (15.1 g, 20.3 mL, 3 equiv.) in DCM (200 mL) was added TIPSC1 (6.75 g, 7.49 mL, 0.90 equiv.) portion wise at 0 °C. The mixture was stirred at 0 °C for 0.5 hr.
• Step E 8-bromo-7-fluoro-3-((triisopropylsilyl)oxy)naphthalen-1-yl trifluoromethanesulfonate: To a solution of 8-bromo-7-fluoro-3-triisopropylsilyloxy-naphthalen-
• Step F ((5-bromo-6-fluoro-4-(trimcthylstannyl)naphthalcn-2- yl)oxy)triisopropylsilane: To a mixture of (8-bromo-7-fluoro-3-triisopropylsilyloxy-1-naphthyl) trifluoromethanesulfonate (5 g, 9.17 mmol, 1 equiv. ⁇ , trimethyl(trimethylstannyl)stannane (9.41 g, 5.96 mL, 3.13 equiv.) and Pd(PPh3) 2 Cb (643 mg, 0.1 equiv.) in toluene (50 mL) was added LiCl (1.17 g, 563 ⁇ L, 3.0 equiv.) under N 2 .
• Step G 7-(8-bromo-7-fluoro-3-((triisopropylsilyl)oxy)naphthalen-1-yl)-8-fluoro-
• Step A ( 8-bromonaphthalen- 1 -yl)methano 1 : To a solution of 8-bromonaphthalene- 1-carboxylic acid (5 g, 1.0 equiv.) in 2-MeTHF (70 mL) was slowly added BH3*Me2S (10 M, 3.0 equiv.) at 0 °C under N 2 . The reaction was stirred at 70 °C for 12 hours. The mixture was quenched with methanol (20 mL) at 0 °C and washed with 1 M HO (30 mL). The mixture was diluted with water (50 ml) and extracted with ethyl acetate (2 x 50 mL).
• Step B 2-((8-bromonaphthalen-1-yl)methoxy)tetrahydro-2H-pyran: To a solution of 3,4-dihydropyran (1.22 g, 1.0 equiv.), 8-bromonaphthalen- l-yl)methanol (3.44 g, 1.0 equiv.) and 4-methylbenzenesulfonic acid (514 mg, 0.1 equiv.) in DCM (40 mL) was stirred at 25 °C for 5 hours. The reaction mixture was concentrated on vacuum.
• Step C 4,4,5,5-tctramcthyl-2-(8-(((tctrahydro-2II-pyran-2- yl)oxy)methyl)naphthalen-] -yl)-1,3,2-dioxaborolane: A mixture of 2-((8-bromonaphthalen-1- yl)methoxy)tetrahydro-2H-pyran (300 mg, 1.0 equiv., 4,4,5,5-tetramethyl-2-(4,4,5,5-tetramethyl- 1 ,3,2-dioxaborolan-2-yl)-1,3,2-dioxaborolane (356 mg, 1.5 equiv.), KOAc (27.5 mg, 0.3 equiv.) and Pd(dppf)Cl 2 (68.3 mg, 0.1 equiv.) in dioxane (1.5 mL) was degassed and stirred at 80 °C for 2 hrs under N 2 atmosphere.
• the combined reaction mixture was diluted with ethyl acetate (10 mL) and water (20 mL), the aqueous layer was extracted with ethyl acetate (10 mL ), the combined organic phase was washed with brine (15 mL), dried over anhydrous sodium sulfate, filtered and concentrated in vacuum.
• Step D 8-fluoro-2-((hexahydro-1H-pyrrolizin-7a-yl)methoxy)-7-(8-(((tetrahydro- 2H-pyran-2-yl)oxy)methyl)naphthalen-1-yl)-4-(2.2.2-trifluoroethoxy)pyrido[4,3-d]pyrimidine: A mixture of 7-chloro-8-fluoro-2-((tetrahydro-1H-pyrrolizin-7a(5H)-yl)methoxy)-4-(2,2,2- trifluoroethoxy)pyrido[4,3-d]pyrimidine (350 mg, 1.0 equiv.), 4,4,5,5-tetramethyl-2-(8- (((tetrahydro-2H-pyran-2-yl)oxy)methyl)naphthalen-1-yl)-1,3,2-dioxaborolane (368 mg, 1.2 equiv.), Cat
• reaction mixture was diluted with ethyl acetate (50 mL) and water (60 mL), extracted with ethyl acetate (30 mL), the combined organic phase was washed with brine (40 mL), dried over anhydrous sodium sulfate, filtered and concentrated in vacuum.
• Step A 5,6-difludro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-2- ol: To a solution of 2-[7,8-difluoro-3-(methoxymethoxy)-1-naphthyl]-4,4,5,5-tetramethyl-l ,3,2- dioxaborolane (500 mg, 1.0 equiv.) in ACN (15 mL) was added HC1dioxane (4 M, 7.50 mL, 21.0 equiv.) at 0 °C. The mixture was stirred at 0 °C for 0.5 hour.
• Step B 5,6-difluoro-4-(8-fluoro-2-(((2R.7aS)-2-fluorohexahydro-1H-pyrrolizin- 7a-yl)methoxy)-4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidin-7-yl)naphthalen-2-ol: To a mixture of 7-chloro-8-fluoro-2-(((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)-4- (2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine (306 mg, 1.0 equiv.) and 5,6-difluoro-4-(4,4,5,5- tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-2-ol (320 mg, 1.50 equiv.)
• Step A (R)-1-(7-chloro-8-fluoro-2-(((S)-1-methylpyrrolidin-2- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol: To a mixture of (R)-1-(2,7- dichloro-8-fluoropyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (2.20 g, 1.0 equiv.) and 4 ⁇ molecular sieves (500 mg) in dioxane (15 mL) was added DIEA (2.58 g, 3.0 equiv.) and [(2S)-1- methylpyrrolidin-2-yl]methanol (1.53 g, 2.0 equiv.).
• Step A 6-bromo-N.N-bis(4-methoxybenzyl)-4-methylpyridin-2-amine: To a solution of 6-bromo-4-methyl-pyridin-2-amine (180 g, 1.0 equiv.) in DMAC (1.8 L) was added NaH (115 g, 60% purity, 3.0 equiv.) at 0 °C under nitrogen atmosphere. The mixture was stirred at 25°C for 1 hour under nitrogen atmosphere. Then 1 -(chloromethyl)-4-methoxy-benzene (331 g, 2.2 equiv.) was added to the reaction mixture at 25 °C. The mixture was stirred at 25 °C for 2 hours.
• Step B N,N-bis(4-methoxybenzyl)-4-methyl-6-(tributylstannyl)pyridin-2-amine: A mixture of 6-bromo- N,N-bis[(4-methoxyphenyl)methyl]-4-methyl-pyridin-2-amine (200 g, 1.0 equiv.), tributyl(tributylstannyl)stannane (651 g, 2.4 equiv.), Pd2(dba)3 (42.9 g, 0.1 equiv.), PCy3 (26.2 g, 0.2 equiv.) and LiCl (99.2 g, 5.0 equiv.) in dioxane (1.8 L) was degassed and stirred at 110 °C for 5 hours under N 2 atmosphere.
• Step D 1-(7-chloro-8-fluoro-2-(((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yI)-3-methylpiperidin-3-ol: A mixture of 1-(2,7-dichloro-8- fluoropyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (2.22 g, 1.0 equiv.), ((2R,7aS)-2- fluorohexahydro-1H-pyrrolizin-7a-yl)methanol (1.60 g, 1.5 equiv.), 4 ⁇ molecular sieves (1.00 g) and DIEA (2.60 g, 3.0 equiv.) in dioxane (30.0 ml) was stirred at 90 °C for 15 hours.
• Step A 4.4,5.5-tetramethyl-2-(8-(methylthio)naphthalen-1-yl)- 1.3.2- dioxaborolane: To a solution of l-bromo-8-methylsulfanyl-naphthalene (1.00 g, 1.0 equiv.) and 4,4,5,5-tetramethyl-1,3,2-dioxaborolane (2.53 g, 5.0 equiv.) in ACN (20 mL) were added TEA (1 .20 g, 3.0 equiv.) and Pd(dppf)CI 2 (433 mg, 0.15 equiv.). The mixture was stirred at 80 °C for 5 hours under N 2 atmosphere.
• Step A l-bromo-2-chloro-4-fluoro-3,5-dimethoxybenzene: To a solution of 5- bromo-2-fluoro-1,3-dimethoxybenzene (50.0 g, 1.0 equiv.) and TMSC1 (2.31 g, 0.1 equiv.) in MeCN (500 mL) was added NCS (34.1 g, 1 .2 equiv.) at 10 °C. The reaction mixture was stirred at 10 °C for 2 hours. The mixture was quenched with saturated brine (500 mL) at 0 °C and extracted with ethyl acetate (3x100 mL).
• Step B 6-fluoro-5.7-dimethoxy-9-methy 1- 1 ,4-dihydro- 1 ,4-epiminonaphthalene: To a mixture of l-bromo-2-chloro-4-fluoro-3,5-dimethoxybenzene (20.0 g, 1.0 equiv.) and 1- methylpyrrole (12.0 g, 2.0 equiv.) in THF (240 mL) was added n-BuLi (2.5 M, 32.65 mL, 1.1 equiv.) at -65 °C. The reaction was stirred at -65 °C for I hour and then at 25 °C for 16 hours.
• Step C. 2 -fluoro- 1,3-dimethoxynaphthalene To a solution of 6-fluoro-5,7- dimethoxy-9-methyl-l ,4-dihydro-1,4-epiminonaphthalene (5.00 g, 1.0 equiv.) in DCM (60.0 mL) was added m-CPBA (5.18 g, 85% purity, 1.2 equiv.) at 0-5 °C. The mixture was stirred at 15 °C for 1 hr. The mixture was quenched with saturated aqueous Na 2 SO 4 solution (100 mL). The organic layer was separated and concentrated under reduced pressure. The residue was purified by column chromatography (SiC>2, petroleum ether/ethyl acetate 1 :0 to 20:1) to afford the tittle compound (2.4 g, 54% yield) as a light yellow oil.
• Step D 2-fluoronaphthalene- 1.3 -diol: To a solution of 2-fluoro-1,3- dimethoxynaphthalene (15.4 g, 1.0 equiv.) in DCM (250 mL) was added BBr 3 (39.3 g, 2.1 equiv.) at -30 °C. The mixture was stirred at -30 °C for 30 minutes. The mixture was quenched with MeOH (2.0 mL) and concentrated under reduced pressure.
• Step E 2-fluoro-8-((triisopropylsilyl)ethynyl)naphthalene-1,3-diol: To a solution of 2-fluoronaphthalene- 1,3-diol (7.6 g, 1.0 equiv.) and 2-bromoethynyl( triisopropyl)silane (13.4 g, 1.2 equiv.) in 1,4-dioxane (150 mL) were added dichloro(p-cymene)ruthenium(Il) dimer (2.61 g, 0.1 equiv.) and AcOK (8.37 g, 2.0 equiv.).
• Step H ((6-((benzyloxy)methoxy) -7-fluoro-8-(4,4,5, 5-tetramethyl- 1,3,2- dioxaborolan-2-yl) naphthalen-1-yl)ethynyl)triisopropylsilane: To a solution of 3-
• Step A tert-butyl 2-(isopropylcarbamoyl)-4.6,7,8-tetrahydropyrazolo[4,3- clazepine-5-carboxylate: To a solution of tert-butyl 4,6,7,8-tetrahydro-2H-pyrazolo[4,3- c]azepine-5-carboxylate (4.0 g, 1.0 equiv.) in THF (20 mL) was added portion wise CDI (2.73 g, 1 equiv.) and isopropylamine (1.1 g, 1.1 equiv.) at 25 °C. The reaction was stirred at 25 °C for 1 hour.
• Intermediate 53 A solution of tert-butyl 2-(isopropylcarbamoyl)-4,6,7,8-tetrahydropyrazolo[4,3- c]azepine-5-carboxylate (6.0 g, 1 equiv.) in
• Step A 5-benzyl-3a-methyltetrahydropyrrolo[3,4-c]pyrrole-1,3(2H.3aH)-dione: To a mixture of 3-methylpyrrole-2, 5-dione (608 mg, 1.0 equiv.) and N-(methoxymethyl)-1- phenyl-N-(trimethylsilylmethyl)methanamine (1.3 g, 1.0 equiv.) in CH 2 Cl 2 (10 mL) was added a solution of TFA (62.4 mg, 40.5uL, 0.1 equiv.) in CH 2 Cl 2 (1.0 mL). The mixture was stirred at 25 °C for 1 hour. The reaction mixture was diluted with sat.
• Step A (R)-tert-butyl (2.3-dihydroxypropyl)carbamate: To a solution of (R)-3- aminopropane-1,2-diol (50.0 g, 1 equiv.) and Et 3 N (84.3 g, 1.52 equiv.) in CH 3 OH (1.5 L) was added BOC2O (182 g, 1.52 equiv.) portion wise at 0 °C. The reaction was stirred at 25 °C for 12 hours. The mixture was poured into H 2 O (2.0 L) and extracted with ethyl acetate (1.0 L).
• Step B (R)-tert-butyl (3-((tert-butyldiphepylsilyl)oxy)-2- hydroxypropyl) carbamate: To a solution of (R)-tert-butyl (2,3-dihydroxypropyl)carbamate (43.0 g, 1 equiv.) in CH 2 Cl 2 (900 mL) were added imidazole (18.37 g, 1.2 equiv.) and TBDPSC1 (67.9 g, 1.1 equiv.). The reaction mixture was stirred at 25 °C for 12 hours. The mixture was partitioned between CH 2 Cl 2 (500 mL) and H 2 O (1.0 L).
• Step C (R)-tert-butyl 2-(((tert-butyldiphenylsilyl)oxy)methyl)-6-methylene-1,4- oxazepane-4-carboxylate: To a solution of tert-butyl N-[(2R)-3-[tert-butyl(diphenyl)silyl]oxy-2- hydroxy-propyl]carbamate (20 g, 1 equiv.) in THF (400 mL) was added NaH (4.10 g, 60% purity, 2.2 equiv.) at 0 °C under N 2 atmosphere followed by 3-chloro-2-(chloromethyl)prop-1-ene (5.82 g, 1 equiv.).
• the reaction was stirred at 0 °C for 2 hours under N 2 atmosphere.
• the mixture was quenched by addition of H 2 O (600 mL) at 0 °C and extracted with ethyl acetate (2x300 mL), the combined organic layers were washed with brine (20 mL) and dried over Na 2 SO 4 .
• the mixture was filtered and concentrated.
• Step D (R)-tert-butyl 2-(((tert-butyldiphenylsilyl)oxy)methyl)-6-oxo-1,4- oxazepane-4-carboxylate: To a solution of tert-butyl (2R)-2-[[tert- butyl(diphenyl)silyl]oxymethyl]-6-methylene-1,4-oxazepane-4-carboxylate (1.8 g, 1 equiv.) in THF (9 mL) and H 2 O (9 mL) were added NalCfi (1.84 g, 2.3 equiv.) and K 2 OsO 4 .2H 2 O (68.8 nig, 0.05 equiv.).
• reaction mixture was stirred at 25 °C for 1 hour.
• the mixture was quenched by addition of aqueous Na 2 SO 3 (20 mL) at 0 °C and H 2 O (10 mL) and then extracted with ethyl acetate (20 mL x 2).
• the combined organic layers were washed with brine (30 mL) and concentrated.
• Step E (2R,6S)-tert-butyl 2-(((tert-butyldiphenylsilyl)oxy)methyl)-6-hydroxy-6- methyl-1,4-oxazepane-4-carboxylate _ and _ (2R,6R)-tert-butyl _ 2-(((tert- butyldiphepylsilyl)oxy)methyl) -6-hydroxy-6-methyl-1,4-oxazepane-4-carboxylate: To a solution of tert-butyl (2R)-2-[[tert-butyl(diphenyl)silyl]oxymethyl]-6-oxo-l ,4-oxazepane-4-carboxylate (900 mg, 1 equiv.) in THF (13 mL) was added MeMgBr (3 M, 4.04 equiv.) under N 2 atm.
• Step F (2R,6R)-2-(hydroxymethyl)-6-methyl-1,4-oxazepan-6-ol: To a solution of (2R.6R)-tert-butyl 2-(((tert-butyldiphenylsilyl)oxy)methyl)-6-hydroxy-6-methyl-1,4-oxazepane- 4-carboxylate (520 mg, 1 equiv.) in CH 2 Cl 2 (2 mL) was added HC1dioxane (4 M, 10 mL). The mixture was stirred at 25 °C for 12 hours.
• Step A tert-butyl 3,3-difluoro-7-azaspiro[4.5]decane-7-carboxylate: To a solution of tert-butyl 3-oxo-7-azaspiro[4.5]decane-7-carboxylate (500 mg, 1.0 equiv.) in CH 2 Cl 2 (8 mL) was added DAST (541 mg, 443 ⁇ L, 1.7 equiv.). The mixture was stirred at 25 °C for 15 hours. The reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (2 x 20 mL).
• Step B 3.3-difluoro-7-azaspiro[4.5]decane: To a solution of tert-butyl 3,3- difluoro-7-azaspiro[4.5]decane-7-carboxy!ate (100 mg, 1.0 equiv.) in dioxane (4 mL) was added HCbdioxane (4 M, 4 mL, 44.1 equiv.). The mixture was stirred at 0 °C for 2 hours. The reaction mixture was concentrated under reduced pressure to remove solvent. The residue was diluted with MeOH (4 mL), the pH was adjusted 9 with NaHCO 3 , the mixture was stirred for 0.3 hours. The reaction mixture was concentrated to afford the title compound (70.0 mg, 91.1% yield, HC1 salt, crude) as a colorless oil.
• Step A tert-butyl (3R,6S)-3-((tert-butyldiphenylsilyl)oxy)-6-hydroxy-2,3,6,7- tetrahydro-1 H-azepine-1-carboxylate: To a solution of tert-butyl 3,6-dihydroxy-2,3,6,7- tetrahydroazepine-1-carboxylate (3.00 g, 1 equiv.) in DMF (20 mL) was added imidazole (2.67 g, 3 equiv.) and TBDPSC1 (4.32 g, 1.2 equiv.). The mixture was stirred at 40 °C for 12 hours. The reaction mixture was concentrated under reduced pressure.
• Step A 5-(2-(3-chloro-4-fluorophenyl)acetyl)-2,2-dimethyl-1.3-dioxane-4,6- dione: To a mixture of 2-(3-chloro-4-fluoro-phenyl)acetic acid (330 g, 1 equiv.) and 2,2-dimethyl- l,3-dioxane-4, 6-dione (277 g, 1.1 equiv.) in MeCN (1500 mL) was added DMAP (18.2 g, 0.09 equiv.) at 20 °C.
• DIEA (86 g, 2.15 equiv.) was added into the mixture slowly over the course of 1 hour under 15-30 °C.
• 2,2-dimethylpropanoyl chloride (232.10 g, 1.1 equiv.) was added into the mixture over the course of 1 hour 1 hour while maintaining the temperature at 25-40 °C.
• the mixture was stirred at 45 °C for 3 hours.
• the mixture was cooled to 0 °C, then the pH was adjusted to 3 with HC1 (4N, 5 L) and mixture was stirred at 0 °C for 1 hour.
• Step B 8-chloro-7-fluoro-1,3-dihydroxy-2-naphthoic acid: A mixture of 5-(2-(3- chloro-4-fluorophenyl)aceLyl)-2,2-dimethyl-1,3-dioxane-4, 6-dione (650 g, 1.0 equiv.) in CF3SO3H (1300 mL) was stirred at 5-20°C. Then the mixture was stirred at 10 °C for 2 hours. After reaction completion, the mixture was poured into ice water (2 L) and filtered. The filter cake was washed with water (5 L) and dried to afford the title compound (2000 g, crude) as yellow solid and used in next step without further purification.
• Step C 8-chloro-7-fluoronaphthalene-1,3-diol: A mixture of 8-chloro-7-fluoro- 1 ,3-dihydroxy-2-naphthoic acid (1.2 kg, 1.0 equiv.) in MeCN (700 mL) and H 2 O (700 mL) was stirred at 85 °C for 12 hours under N 2 . The mixture was concentrated under vacuum to remove acetonitrile. The residue was extracted with ethyl acetate (2 Lx 2), dried over Na2SO ⁇ and concentrated in vacuum.
• Step D 8-chloro-7-fluoro-3-((triisopropylsilyl)oxy)naphthalen-1-ol: To a solution of 8-chloro-7-fluoronaphthalene-1,3-diol (10 g, 1 equiv.) and DIEA (12.2 g, 2.0 equiv.) in DCM (150 mL) was added TIPSC1 (8.16 g, 0.9 equiv.) at 0 °C. The mixture was stirred at 0 °C for 0.5 hour. The mixture was concentrated in vacuum and the residue was purified by column chromatography (SiO 2 , petroleum ether / ethyl acetate 10:1) to afford the title compound (15 g, 86% yield) as a yellow oil.
• Step E 8-chloro-7-fluoro-3-((triisopropylsilyl)oxy)naphthalen-1-yl trifluoromethanesulfonate: To a mixture of 8-chloro-7-fluoro-3-
• Example F ((5-chloro-6-fluoro-4-(trimethylstannyl)nanhthalen-2- yl)oxy)triisopropylsilane: To a mixture of 8-chloro-7-fluoro-3-((triisopropylsilyl)oxy)naphthalen- 1 -yl trifluoromethanesulfonatc (5 g, 1.0 equiv.), trimethyl(trimethylstannyl)stannane (12.7 g, 3.88 equiv.) and LiCl (1.27 g, 3.0 equiv.) in toluene (50 mL) was added Pd(PPh3)4 (1.15 g, 0.1 equiv.) under N 2 .
• Pd(PPh3)4 (1.15 g, 0.1 equiv.
• Step A benzyl (R)-2-thia-l ,3,7-tfiazaspiro[4.5]decane-7-carboxylate 2,2-dioxide and benzyl (S)-2-thia-1,3,7-triazaspiror4.5]decane-7-carboxylate 2,2-dioxide: Racemic benzyl 2- thia-1,3,7-triazaspiro[4.5]decane-7-carboxylate 2,2-dioxide (46.5 g) was separated by chiral SFC (column: DAICEL CHIRALPAK IC(250mm*50mm,10um); mobile phase: [0.1%NH3H 2 O-IPA]; B%: 55%-55%, 7min) to afford the two title compound isomers: R-isomer (22 g, 41% yield, 99.9%ee) as a yellow solid and S-isomer (22 g, 42% yield, 99.9%ee) as a yellow solid
• Step B (R)-2-thia-1.3.7-triazaspiro[4.5]decane 2,2-dioxide: To a solution of (R) -2- thia-1,3,7-triazaspiro[4.5]decane-7-carboxylate 2,2-dioxide (20.0 g, 1.00 equiv.) in methanol (160 mL) was added Pd/C (10%, 3.00 g) under nitrogen atmosphere. The suspension was degassed and stirred under hydrogen (15 Psi) at 25 °C for 2 hours.
• (S)-2-thia-1,3,7-triazaspiro[4.5]decane 2,2-dioxide To a solution of benzyl benzyl (S)-2-thia-l ,3,7-triazaspiro[4.5]decane-7-carboxylate 2,2-dioxide. (22.0 g, 1.00 equiv.) in methanol (160 mL) was added Pd/C (10%, 3.00 g) under nitrogen atmosphere. The suspension was degassed and stirred under hydrogen (15 Psi) at 25 °C for 2 hours. The reaction mixture was filtered and concentrated under reduced pressure to afford the title compound (11.8 g, 91% yield) as a yellow solid.
• Step A 3-chloro-4-cyclopropyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2- yl)phenol: To a solution of 3-bromo-5-chloro-4-cyclopropyl-phenol (2.00 g, 1.0 equiv.) in dioxane (50 mL) was added KOAc (2.38 g, 3.0 equiv.), Pin 2 B 2 (4.00 g, 2.0 equiv.) and Pd(dppf)Cli (591 mg, 0.1 equiv.). The mixture was stirred at 100 °C for 4 hours under nitrogen atmosphere.
• reaction mixture was poured into saturated aqueous NH 4 Cl solution (5 mL) and extracted with EtOAc (3 x 2 mL). The combined organic layers were washed with brine (5 mL), dried over anhydrous sodium sulfate, filtered and concentrated.
• Step A 6-(7-chloro-8-fluoro-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-l .6-diazaspiro[3.5]nonan-2-one: To a mixture of 7- chloro-8-fluoro-2-((tetrahydro-1H-pyrrolizin-7a(5H)-yl)methoxy)-4-(2,2,2- trifluoroethoxy)pyrido[4,3-d]pyrimidine (200 mg, 475 ⁇ mol, 1.0 equiv.), 1,6- diazaspiro[3.5]nonan-2-one (60.0 mg, 428 ⁇ mol, 0.9 equiv.), 4 ⁇ molecular sieves (10.0 mg) in DMF (2 mL) was added DIEA (184 mg, 1.43 mmol, 248 ⁇ L, 3.0
• Step A 4-(7-chloro-8-fluoro-2-((tetrahydro-1H-pyrrolizin-7a(5H)- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-6-methyl-1,4-oxazepan-6-ol: A mixture of 7-chloro-8- fhioro-2-((tetrahydro-1H-pyrrolizin-7a(5H)-yl)methoxy)-4-(2,2,2-trifluoroethoxy)pyrido[4,3- d]pyrimidine (0.45 g, 1 equiv.), 6-methyl-1,4-oxazepan-6-ol (210 mg, 1.5 equiv.), DIPEA (276 mg, 2 equiv.) and 4 A molecular sieves (50 mg) in DMF (4 mL) was stirred at 40 °C for 14 hours under N2 atmosphere.
• the mixture was stirred at - 40 °C for 0.5 h. After completion, the mixture was quenched by water (10 mL). The aqueous phase was extracted with dichloromethane (2 x 8 mL), the combined organic layer was washed with brine (10 mL) and dried over with Na 2 SO 4 . The mixture was filtered and concentrated in vacuum.
• Step B 1-(7-(8-chloronaphthalen-1-yl)-8-fluoro-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol: To a mixture of l-[2-chloro-7- (8-chloro-1-naphthyl)-8-fluoro-pyrido[4,3-d]pyrimidin-4-yl]-3-methyl-piperidin-3-ol (112 mg, 245 ⁇ mol) and (tetrahydro- 1H-pyrrolizin-7a(5H)-yl)methanol (69.2 mg, 490 ⁇ mol) in dioxane (2.0 mL) was added DIEA (95.0 mg, 735 ⁇ mol, 128 ⁇ L).
• Step A 7-[2-chloro-7-(8-chloro-1-naphthyl)-8-fluoro-pyrido[4,3-d]pyrimidin-4- yl]-2,7-diazaspiro[4.5]decan-3-one: To a mixture of 2,4-dichloro-7-(8-chloro-l -naphthyl)-8- fluoro-pyrido[4,3-d]pyrimidine (0.15 g, 396 ⁇ mol) in dichloromethane (5.0 mL) was added DIEA (512 mg, 3.96 mmol) at - 40 °C.
• Step B 7-(7-(8-chloronaphthalen-1-yl)-8-fluoro-2-((tetrahydro-1H-pyrrolizin- 7a(5H)-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-2.7-diazaspiro[4.5]decan-3-one: A mixture of 7-[2-chloro-7-(8-chloro-1-naphthyl)-8-fluoro-pyrido[4,3-d]pyrimidin-4-yl]-2,7- diazaspiro[4.5]decan-3-one (100 mg, 201 ⁇ mol), (tetrahydro-1H-pyrrolizin-7a(5.H)-yl)methanol (56.9 mg, 403 ⁇ mol) and DIEA (130 mg, 1.01 mmol, 175 ⁇ L) in dioxane (1.0 mL) was stirred at 80 °C
• Step A 5-(8-fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-l ,5-diazocan-2-one: To a solution of 8-fluoro-7-(8- fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a-yl)methoxy)-4-(2,2,2- trifluoroethoxy)pyrido[4,3-d]pyrimidine (100 mg, 188 ⁇ mol), 4 ⁇ MS (50 mg) and 1 ,5-diazocan- 2-one (48.3 mg, 377 ⁇ mol) in DMF (2.00 mL) was added DIEA (73.1 mg, 565 ⁇ mol).
• Step A (S)-1-(7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro-
• Step B (S)-1-(7-(8-ethyl-7-fluoro-3-hydroxynaphthalen-1-yl)-8-fluoro-2- (((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3- methylpiperidin-3-ol: To a solution of 2-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)- 4,4,5,5-tetramethyl-1,3,2-dioxaborolane (70 mg, 107 ⁇ mol, 1 equiv.) in MeCN (1.4 mL) was added HC1dioxane (4 M, 1.4 mL, 52 equiv.) at 5 °C.
• reaction mixture was stirred at 5 °C for 0.5 hour. Upon completion, the reaction mixture was diluted with MeCN (3 mL) and basified with saturated NaHCO3 solution to pH > 7. The reaction mixture was extracted with ethyl acetate (3 x 5 mL). The combined organic layers were dried over Na2SO4, filtered and concentrated in vacuum.
• Step A (R)-1-(7-chloro-8-fluoro-2-(((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin- 7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol: To the mixture of (R)-1- (2,7-dichloro-8-fluoropyrido[4,3-d]pyrimidin-4-yl)-3-rnethylpiperidin-3-ol (20.0 g, 60.4 mmol), ((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a-yl)methanol (10.6 g, 66.4 mmol), 4 ⁇ molecular sieves (5.00 g) in dioxane (80 mL) was added DIEA (23.4 g, 181 mmol), and the mixture was stirred at 90 °C for
• reaction mixture Upon completion, the reaction mixture was filtered. The mixture was diluted with ethyl acetate (300 mL) and water (200 mL), and aqueous layer was then extracted with ethyl acetate (300 mL). The combined organic phase was washed with' brine (200 mL) and dried over anhydrous sodium sulfate. The mixture was filtered and concentrated to give a residue.
• Step B (R)-1-(7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro-
• reaction mixture was diluted with ethyl acetate (300 mL) and water (200 mL). The aqueous layer was extracted with ethyl acetate (300 mL). The combined organic phase was washed with brine (200 mL) and dried over anhydrous Na2SO4. The mixture was filtered and concentrated to give a residue.
• Step C (R)-1-(7-(8-ethyl-7-fluoro-3-hydroxynaphthalen-1-yl)-8-fluoro-2- (((2R.7aS)-2-fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3- methylpiperidin-3-ol: To the solution of (R)-1-(7-(8-ethyl-7-fluoro-3-
• Step A 2-(((2S,4R)-4-((tert-butyldiphepylsilyl)oxy)-1-methylpyrrolidin-2- yl)methoxy)-7-chloro-8-fluoro-4-(piperidin-1-yl)pyrido[4.3-d]pyrimidine: A solution of 2,7- dichloro-8-fluoro-4-(piperidin-1-yl)pyrido[4,3-d]pyrimidine (140 mg, 465 ⁇ mol) and ((2S,4R)-4- ((tert-butyldiphenylsilyl)oxy)-1-methylpyrrolidin-2-yl)methanol (189 mg, 511 ⁇ mol) in toluene (3.00 mL) was added tBuONa (134 mg, 1.39 mmol).
• Step B 2-(((2S.4R)-4-((tert-butyldiphenylsilyl)oxy)-1-methyl pyrrolidin-2- yl)methoxy)-7-( 8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen- 1 -yl)-8-fluoro-4-(piperidin- 1 - yl)pyrido[4,3-d]pyrimidine: A mixture of 2-(((2S,4R)-4-((tert-butyldiphenylsilyl)oxy)-1- methylpyrrolidin-2-yl)methoxy)-7-chloro-8-fluoro-4-(piperidin-1-yl)pyrido[4,3-d]pyrimidine (170 mg, 268 ⁇ mol), 2-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-4,4,5,5-
• Step C (3R,5S)-5-(((7-(8-ethyl-7-fluoro-3-(methoxymethoxy3naphthalen-1-yl)-8- fluoro-4-(piperidin-1-yl)pyrido[4,3-d]pyrimidin-2-yl)oxy)methyl)-1-methylpyrrolidin-3-ol:
• 2-(((2S,4R)-4-((tert-butyldiphenylsilyl)oxy)-1-methylpyrrolidin-2-yl)methoxy)-7-(8- ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro-4-(piperidin-1-yl)pyrido[4,3- d]pyrimidine 200 mg, 144 ⁇ mol, 60% purity) in DMF (6.0 mL) was added CsF (65.7 mg, 433 ⁇ mol, 15.
• Step D (3R,5S)-5-(((7-(8-ethyl-7-fluoro-3-hydroxynaphthalen-1-yl)-8-fluoro-4- (piperidin-1-yl)pyrido[4,3-d]pyrimidin-2-yl)oxy)methyl)-1-methylpyrrolidin-3-ol: A solution of (3R,5S)-5-(((7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1 -yl)-8-fluoro-4-(piperidin-1- yl)pyrido[4,3-d]pyrimidin-2-yl)oxy)methyl)-1-methylpyrrolidin-3-ol (80.0 mg, 135 ⁇ mol) in HC1EtOAc (4 M, 1 mL) was stirring at 20 °C for 1 hour.
• Step A 2-(((2R,7aS)-2-((tert-butyldiphenylsilyl)oxy)hexahydro-1H-pyrrolizin-7a- yl)methoxy)-7-chloro-8-fluoro-4-(piperidin-1-yl)pyrido[4,3-d]pyrimidine: A solution of 2,7- dichloro-8-fluoro-4-(l -piperidyl)pyrido[4,3-d]pyrimidine (200 mg, 664 ⁇ mol) and ((2R,7aS)-2- ((tert-butyldiphenylsilyl)oxy)tetrahydro-1H-pyrrolizin-7a(5H)-yl)methanol (315 mg, 797 ⁇ mol) in toluene (3.00 mL) was added drop- wise t-BuONa (191 mg, 1.99 mmol).
• the mixture was stirred at 0 °C for 1 hour under N 2 .
• the reaction mixture was quenched by the addition of water (50.0 mL) at 0 °C, and extracted with DCM (30.0 mL x 3). The combined organic layers were washed with brine (40.0 mL), dried over Na 2 SO 4 , filtered and concentrated in vacuum.
• Step B 2-(((2R,7aS)-2-((tert-butyldiphenylsilyl)oxy)hexahydro-1H-pyrrolizin-7a- yl)methoxy)-7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro-4-(piperidin-1- yl)pyrido[4,3-d]pyrimidine: To a solution of 2-(((2R,7aS)-2-((tert- butyldiphenylsilyl)oxy)hexahydro-1H-pyrrolizin-7a-yl)methoxy)-7-chloro-8-fluoro-4-(piperidin- l -yl)pyrido[4,3-d]pyrimidine (240 mg, 363 ⁇ mol), and 2-(8-ethyl-7-fluoro-3- (methoxymethoxy)
• Step C (2R.7aS)-7a-(((7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)- 8-fluoro-4-(piperidin-1-yl)pyrido[4,3-d]pyrimidin-2-yl)oxy)methyl)hexahydro-1H-pyrrolizin-2- ol: To a solution of 2-(((2R,7aS)-2-((tert-butyldiphenylsilyl)oxy)hexahydro-1H-pyrrolizin-7a- y l)methoxy)-7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen- 1 -yl)-8-fluoro-4-(piperidin- 1 - yl)pyrido[4,3-d]pyrimidine (250 mg, 262 ⁇ mol, 90% purity) in DMF (1
• Step D (2R,7aS)-7a-(((7-(8-ethyl-7-fluoro-3-hydroxynaphthalen-1-yl)-8-fluoro-4- (piperidin-1-yl)pyrido[4,3-d]pyrimidin-2-yl)oxy)methyl)hexahydro-1H-pyrrolizin-2-ol: To a solution of (2R,7aS)-7a-(((7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro-4- (piperidin-1-yl)pyrido[4,3-d]pyrimidin-2-yl)oxy)methyl)hexahydro-1H-pyrrolizin-2-ol (100 mg, 129 ⁇ mol, 80% purity) in EtOAc (1.00 mL) was added drop-wise HCI/EtOAc (4 M, .1.00 mL).
• Step A 2,7-dichloro-8-fluoro-4-((3S)-3-methyl-3-((tetrahydro-2H-pyran-2- y l)oxy)pi peridin- 1 -yl)pyrido[4,3-d] pyrimidine: To the mixture of (S)-1-(2,7-dichloro-8- fluoropyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (2.00 g, 6.04 mmol), TsOH WATER (115 mg, 605 ⁇ mol) in dichloromethane (30 mL) was added 3,4-dihydro-2H-pyran (1.02 g, 12.1 mmol) at 0°C.
• Step C 2-(((2S.4R)-4-((tert-butyldiphenylsilyl)oxy)-1-methylpyrrolidin-2- yl)methoxy)-7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro-4-((3S)-3- methyl-3-((tetrahydro-2H-pyran-2-yl)oxy)piperidin-1-yl)pyrido[4.3-d]pyrimidine: To the mixture of 2-(((2S,4R)-4-((tert-butyldiphenylsilyl)oxy)-1-methylpyrrolidin-2-yl)methoxy)-7-chloro-8- fluoro-4-((3S)-3-methyl-3-((tetrahydro-2H-pyran-2-yl)oxy)piperidin-1-yl)pyrido[4,3- d]
• Step D (3R,5S)-5-(((7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8- fluoro-4-((3S)-3-methyl-3-((tetrahydro-2H-pyran-2-yl)oxy)piperidin-1-yl)pyrido[4.3- d1pyrimidin-2-yl)oxy)methyl)-1-methylpyrrolidin-3-ol: To the mixture of 2-(((2S,4R)-4-((tert- butyldiphenylsilyl)oxy)-1-methylpyrrolidin-2-yl)methoxy)-7-(8-ethyl-7-fluoro-3- (methoxymethoxy)naphthalen-1-yl)-8-fluoro-4-((3S)-3-methyl-3-((tetrahydro-2H-pyran-2- yl)oxy)piperidin-1-
• Step E (3R,5S)-5-(((7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8- fluoro-4-((3S)-3-methyl-3-((tetrahydro-2H-pyran-2-yl)oxy)piperidin- 1 -yl)pyrido[4,3- d1pyrimidin-2-yl)oxy)methyl)-1-methylpyrrolidin-3-yl (4-nitrophenyl) carbonate: To the mixture of (3R,5S)-5-(((7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro- 1 -((3S)-3- methyl-3-((tetrahydro-2H-pyran-2-yl)oxy)piperidin-1-yl)pyrido[4,3-d]pyrimidin-2- yl
• Step F [(3R,5S)-5-[[7-[8-ethyl-7-fluoro-3-(methoxymethoxy)-1-naphthyl]-8- fluoro-4-[(3S)-3-methyl-3-tetrahydropyran-2-yloxy-1-piperidyl]pyrido[4.3-d]pyrimidin-2- yl]oxymethyl]-1-methyl-pyrrolidin-3-yl] N-methylcarbamate: A mixture of (3R,5S)-5-(((7-(8- ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro-4-((3S)-3-methyl-3-((tetrahydro- 2H-pyran-2-yl)oxy)piperidin-1-yl)pyrido[4,3-d]pyrimidin-2-yl)oxy)methyl)-1-methylpyrrolidin- 3-
• Step A (S)-1-(7-chloro-8-fluoro-2-((2R,7aS)-2-fhjorohexahydro-.1H-pyrrolizin- 7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol: A mixture of (S)-1-(2,7- dichloro-8-fluoropyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (300 mg, 906 ⁇ mol), ((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a-yl)methanol (150 mg, 942 ⁇ mol), DIPEA (311 mg, 2.41 mmol) and 4 ⁇ molecular sieves (150 mg) in dioxane (1.8 mL) was stirred at 90 °C for 24 hours under N 2 atmosphere.
• Step B (S)-1-(8-fluoro-7-(7-fluoro-3-(methoxymethoxy)-8-
• Step C ( S)- 1 -(7-( 8-ethynyl-7-fluoro-3-( methoxymethoxylnaphthalen- 1 -yl)-8- fluoro-2-(((2R.7aS)-2-fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4- yl)-3-methylpiperidin-3-ol: To a solution of (S)-1-(8-fluoro-7-(7-fluoro-3-(methoxymethoxy)-8- ((triisopropylsilyl)ethynyl)naphthalen-1-yl)-2-(((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin
• Step D (S)- 1 -(7-(8-ethynyl-7-fl uoro-3 -hydroxynaphthalen- 1 -yl)- 8-fluoro-2-
• Step A 1-(8-fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyirolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)piperidin-4-ol: To a solution of 8-fluoro-7-(8- fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a-yl)methoxy)-4-(2,2,2- trifluoroethoxy)pyrido[4,3-d]pyrimidine (50.0 mg, 94.3 ⁇ mol) in DMF (2.00 mL) were added piperidin-4-ol (19.1 mg, 189 ⁇ mol) and D1EA (36.5 mg, 283 ⁇ mol, 49.3 ⁇ L).
• reaction was degassed and purged with N 2 for 3 times and stirred at 40 °C for 2 hours. Upon completion, the reaction was filtered and purified by prep-HPLC (column: water s Xbridge BEH C18 100x25mmx5 ⁇ m; mobile phase: [water (10 mM NH 4 HCO 3 )-ACN]; B%: 20%-60%, 10 min) affording the title compound (24.1 mg, 48% yield, 99.9% purity) as a white solid.
• Step A 2,7-dichloro-8-fluoro-4-((3R)-3-methyl-3-((tetrahydro-2H-pyran-2- yl)oxy)piperidin-1-yl)pyrido[4,3-d]: A mixture of (R)-1-(2,7-dichloro-8-fluoropyrido[4,3- d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (2.00 g, 6.04 mmol), TsOH.H2O (115 mg, 604 ⁇ mol) and DHP (1.02 g, 12.1 mmol, 1.10 mL) in dichloromethane (20 mL) was stirred at 15 °C for 1 h.
• Step B tert-butyl-[(3R,5S)-5-[[7-chloro-8-fluoro-4-[(3R)-3-methyl-3- tetrahydropyran-2-yloxy-1-piperidyl]pyrido[4,3-d]pyrimidin-2-yl]oxymethyl]-1-methyl- pyrrolidin-3-yl]oxy-diphenyl-silane: A mixture of 2,7-dichloro-8-fluoro-4-[(3R)-3-methyl-3- tetrahydropyran-2-yloxy-1-piperidyl]pyrido[4,3-d]pyrimidine (2 g, 4.82 mmol), [(2S,4R)-4-[tert- butyl(diphenyl)silyl]oxy -1-methyl-pyrrolidin-2-yl]methanol (3.56 g, 9.63 mmol) and DIEA (1.87 g, 14.5
• Step C 2-(((2S,4R)-4-((tert-butyldiphenylsilyl)oxy)-1-methylpyrrolidin-2- yl)methoxy)-7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro-4-((3R)-3- methyl-3-((tetrahydro-2H-pyran-2-yl)oxy)piperidin-1-yl)pyrido[4,3-d]pyrimidine: To a mixture of tert-butyl-[(3R,5S)-5-[[7-chloro-8-fluoro-4-[(3R)-3-methyl-3-tetrah'ydropyran-2-yloxy-1- piperidyl]pyrido[4,3-d]pyrimidin-2-yl]oxymethyl]-1-methyl-pyrrolidin-3-yl]oxy-diphenyl
• Step D (3R,5S)-5-(((7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8- fluoro-4-((3R)-3-methyl-3-((tetrahydro-2H-pyran-2-yl)oxy)piperidin-1-yl)pyrido[4,3- dlpyrimidin-2-yl)oxy)methyl)-1-methylpyrrolidin-3-ol: To a mixture of 2-(((2S,4R)-4-((tert- butyldiphenylsiiyl)oxy)-1-methylpyrrolidin-2-yl)methoxy)-7-(8-ethyl-7-fluoro-3- (methoxymethoxy)naphthalen-1-yl)-8-fluoro-4-((3R)-3-methyl-3-((tetrahydro-2H-pyran-2- yl)oxy)piperidin-1-y
• Step E [(3R,5S)-5-[[7-[8-ethyl-7-fluoro-3-(methoxymethoxy)-1-naphthyl]-8- fluoro-4-[(3R)-3-methyl-3-tetrahydropyran-2-yloxy-1-piperidyl]pyrido[4,3-d]pyrimidin-2- yl]oxymethyl]-1-methyl-pynolidin-3-yl] (4-nitrophenyl) carbonate: To a mixture of (3R,5S)-5- (((7-(8-ethyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro-4-((3R)-3-methyl-3- ((tetrahydro-2H-pyran-2-yl)oxy)piperidin-1-yl)pyrido[4,3-d]pyrimidin-2-yl)oxy)methyl)-1- methyl
• Step F [(3R,5S)-5-[[7-[8-ethyl-7-fluoro-3-(methoxymethoxy)-1-naphthyri-8- fluoro-4-[(3R)-3-methyl-3-tetrahydropyran-2-yloxy-1-piperidyl]pyrido[4,3-d]pyrimidin-2- yl]oxymethyll-1-methyl-pyrrolidin-3-yl] N-methylcarbamate: A mixture of [(3R,5S)-5-[[7-[8- ethyl-7-fluoro-3-(methoxymethoxy)-1-naphthyl]-8-fluoro-4-[(3R)-3-methyl-3-tetrahydropyran- 2-yloxy-1-piperidyl]pyrido[4,3-d]pyrimidin-2-yl]oxymethyl]-1-methyl-pyrrolidin-3-yl] (4-
• Step G [(3R,5S)-5-[[7-(8-ethyl-7-fluoro-3-hydroxy-1-nanhthyl)-8-fluoro-4-[(3R)-
• Step A (R)- 1 -(7-chloro-8-fluoro-2-(((2R,7aS)-2-fluorohexahydro- 1 H-pyrrolizin- 7a-yl)methoxy)pyrido
• Step B (R)-1-(8-fluoro-7-(7-fluoro-3-(methoxymethoxy)-8-
• Step C (R)-1-(7-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8- fluoro-2-(((2R,7aS)-2-fluorohexahydro-1H-pyiTolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4- yl)-3-methylpiperidin-3-ol: To a solution of (R)-1-(8-fluoro-7-(7-fluoro-3-(methoxymethoxy)-8- ((triisopropylsilyl)ethynyl)naphthalen-1-yl)-2-(((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-
• Step D (R)-1-(7-(8-ethynyl-7-fluoro-3-hydroxynaphthalen-1-yl)-8-fluoro-2- (((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3- methylpiperidin-3-ol: To a mixture of (R)-1-(7-(8-ethynyl-7-fluoro-3-
• Step A (R)-1-(7-chloro-8-fluoro-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol: To a mixture of (R)-1-(2,7- dichloro-8-fluoropyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (400 mg, 1.21 mmol), (hexahydro-1H-pyrrolizin-7a-yl)methanol (239 mg, 1.69 mmol), 4 ⁇ molecular sieves (40.0 mg) in dioxane (8 mL) was added DIEA (468 mg, 3.62 mmol), the mixture was stirred at 90 °C for 3 hours.
• Step B (R)-1-(8-fluoro-7-(7-fluoro-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)- 2-((hexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3- ol: To the solution of (R)-1-(7-chloro-8-fluoro-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (200 mg, 459 ⁇ mol), ((2-fluoro- 8-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropyl
• Step C (R)-1-(7-(8-ethynyl-7-fluoronaphthalen-1-yl)-8-fluoro-2-((hexahydro-1H- Pyrrolizin-7a-yl)methoxy)pyrido[4.3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol: To the solution of (R)-1-(8-fluoro-7-(7-fluoro-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-2-((hexahydro-1H- pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (100 mg, 138 ⁇ mol) in DMF (1.5 mL) was added CsF (209 mg, 1.38 mmol, 10 equiv.).
• Step A (R)-1-(6-(8-ethyl-7-fluoronanhthalen-1-yl)-5-fiuoro-3-((hexahydro-1H- pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-1-yl)-3-methylpiperidin-3-oI: To a solution of (R)-1-(7-(8-ethynyl-7-fluoronaphthalen-1-yl)-8-fluoro-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (50.0 mg, 87.8 ⁇ mol) in MeOH (2 mL) was added Pd/C (20 mg, 10% purity) under N 2 .
• Step B (R)-1-(8-fluoro-7-(7-fluoro-8-((triisopronylsilyl)ethynyl)naphthalen-1-yl)-
• Step C (R)-1-(7-(8-ethynyl-7-fluoronaphthalen-1-yl)-8-fluoro-2-(((2R,7aS)-2- fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-
• Step D (R)-1-(7-(8-ethyl-7-fluoronaphthalen- l-yl)-8-fluoro-2-(((2R,7aS)-2- fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin- 3-ol: To a solution of (R)-1-(7-(8-ethynyl-7-fluoronaphthalen-1-yl)-8-fluoro-2-(((2R,7aS)-2- fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin- 3-ol (50.0 mg, 85.1 ⁇ mol) in MeOH (2.0 mL) was added Pd/C (20 mg, 10% purity) under N 2 .
• Step A 1-(8-fluoro-7-(8-fluoronaphthalen-1-y l)-2-((hexahydro- 1 H-pyrrolizin- 7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol: To a mixture of 3- methyipiperidin-3-ol and DIEA (51.1 mg, 396 ⁇ mol) and 4 ⁇ molecular sieves (50 mg) in DMF (2 ml) was added 8-fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin- 7a- yl)methoxy)-4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine (69.9 mg, 132 ⁇ mol) in one portion at 20 °C under N 2 .
• Step A 4-((lR,5S)-3-azabicyclo[3.2.1]octan-3-yl)-8-fluoro-7-(8- fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidine: To a solution of 8-fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a-yl)methoxy)- 4-(2,2,2-trifluoroethoxy)pyrido[4,3-d]pyrimidine (50.0 mg, 94.3 ⁇ mol) in DMF (1.0 m.L) were added DIEA (36.5 mg, 283 ⁇ mol, 49.3 ⁇ L) and (lR,5S)-3-azabicyclo[3.2.1]octane (31.4 mg, 283 ⁇ mol).
• Step A 8-fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)-N-((l-methylcyclobutyl)methyl)pyrido[4,3-d]pyrimidin-4-amine: To a solution of 8- fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a-yl)methoxy)-4-(2,2,2- trifluoroethoxy)pyrido[4,3-d]pyrimidine (50.0 mg, 94.3 ⁇ mol) in DMF (1.0 mL) were added DIEA (60.9 mg, 471 ⁇ mol, 82.1 ⁇ L) and (l-methylcyclobutyl)methanamine (38.4 mg, 283 ⁇ mol, HC1).
• Step A 7-(8-fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a- yl) methoxy)pyrido[4,3-d]pyrimidin-4-yl)-2,7-diazaspiro[4.5]decan-3-one: To a mixture of 8- fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a-yl)methoxy)-4-(2,2,2- trifluoroethoxy)pyrido[4,3-d]pyrimidine (50.0 mg, 94.3 ⁇ mol), DIP.A (60.9 mg, 471 ⁇ mol, 82.1 ⁇ L) and 4 ⁇ molecular sieves (5.0 mg) in DMF (2.0 mL) was added 2,7-diazaspiro[4.5]decan-3- one (44.9 mg, 236 ⁇ mol
• Step A 8-fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)-4-(piperidin-1-yl)pyrido[4,3-d]pyrimidine: To a solution of 8-fluoro-7-(8- fluoronaphthalen- 1 -yl)-2-((hexahydro- 1H-pyrrolizin-7a-yl)methoxy)-4-(2,2,2- trifluoroethoxy)pyrido[4,3-d]pyrimidine (50.0 mg, 94.3 ⁇ mol) in DMF (1.0 mL) were added DIEA (36.5 mg, 283 ⁇ mol, 49.3 ⁇ L) and piperidine (12.0 mg, 141 ⁇ mol, 14.0 ⁇ L).
• Step A 8-fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)-4-(3-methylpiperidin-1-yl)pyrido[4,3-d]pyrimidine: To a solution of 8-fluoro-7-(8- fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a-yl)methoxy)-4-(2,2,2- trifluoroethoxy)pyrido[4,3-d]pyrimidine (50.0 mg, 94.3 ⁇ mol) in DMF (1 .0 mL) were added DIEA (36.5 mg, 283 ⁇ mol, 49.3 ⁇ L) and 3-methylpiperidine (28.0 mg, 283 ⁇ mol , 33.2 ⁇ L).
• Step A 1-(8-fluoro-7-(8-fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)piperidin-3-ol: To a solution of 8-fluoro-7-(8- fluoronaphthalen-1-yl)-2-((hexahydro-1H-pyrrolizin-7a-yl)methoxy)-4-(2,2,2- trifluoroethoxy)pyrido[4,3-d]pyrimidine (50.0 mg, 94.3 ⁇ mol) in DMF (1.0 mL) were added DLEA (36.5 mg, 283 ⁇ mol, 49.3 ⁇ L) and piperidin-3-ol (28.6 mg, 283 ⁇ mol, 33.2 ⁇ L).
• Step A 7-(2,7-dichloro-8-fluoropyrido[4.3-d]pyrimidin-4-yl)-2.7- diazaspiro[4.5]decan-3-one: To a solution of 2,4,7-trichloro-8-fluoropyrido[4,3-d]pyrimidine (2.0 g, 6.85 mmol, 86% purity) in dichloromethane (20 mL) were added DIEA (3.54 g, 27.4 mmol, 4.77 mL) and 2,7-diazaspiro[4.5]decan-3-one (1.44 g, 7.53 mmol, HO) at -40 °C. The mixture was stirred at -40 °C for 1 h.
• Step B 7-(7-chloro-8-fluoro-2-(((2R.7aS)-2-fluorotetraliydro-1H-pyrrolizin- 7a(5H)-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-2.7-diazaspiro[4.5]decan-3-one: To a mixture of 7-(2,7-dichloro-8-fluoropyrido[4,3-d]pyrimidin-4-yl)-2,7-diazaspiro[4.5]decan-3- one (300 mg, 810 ⁇ mol), DIEA (314 mg, 2.43 mmol, 423 ⁇ L) and 4 ⁇ molecular sieves (10 mg) in dioxane (2.0 mL) was added ((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin- 7a-yl)methanol (142 mg, 891 ⁇
• Step D 7-(7-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-8-fluoro- 2- (((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-2,7- diazaspiro[4.5]decan-3-one: To a solution of 7-(8-fluoro-7-(7-fluoro-3- (methoxymethoxy)-8- ((triisopropylsilyl)ethynyl)naphthalen-1-yl)-2-(((2R,7aS)-2-fluorohexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-2,7-diazaspiro
• Step E 7-(7-(8-ethynyl-7-fluoro-3-hydroxynaphthalen-1-yl)-8-fluoro-2- (((2R cramp7aS)-2- fluorohexahydro-1H-pyrrolizin-7a-yl)methoxy)pyrido[4.,3-d]pyrimidin-4-yl)-2,7- diazaspiro[4.5]decan-3-one: To a solution of 7-(7-(8-ethynyl-7-fluoro-3-
• Step A 5,6-dimethyl-1-tetrahydropyran-2-yl-4-(4,4,5,5-tetramethyl-1,3,2- dioxaborolan-2-yl)indazole: To a solution of 4-bromo-5,6-dimethyl-1-(tetrahydro-2H-pyran-2- yl)-1H-indazole (2.00 g, 6.47 mmol), 4,4,5,5-tetramethyl-2-(4,4,5,5-tetramethyl-1,3,2- dioxaborolan-2-yl)-1,3,2-dioxaborolane (3.29 g, 12.9 mmol) and KOAc (1.90 g, 19.4 mmol) in dioxane (40 mL) was added Pd(dppf)CI 2 (473 mg, 647 ⁇ mol).
• Step B 1 -(7-(5,6-dimethyl-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-4-yl)-8- fluoro-2-((tetrahydro-1H-pyrrolizin-7a(5H)-yl)methoxy)pyrido[4.3-d]pyrimidin-4-yl)-3- methylpiperidin-3-ol: To a mixture of 1-(7-chIoro-8-fIuoro-2-((hexahydro-1H-pyrrolizin-7a- yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol(290 mg, 665 ⁇ mol) and 5,6- dimethyl-1-tetrahydropyran-2-yl-4-(4,4,5,5-tetrainethyl-1,3,2-dioxaborolan-2-yl)indazole (3
• Step C 1 -(7-(5,6-dimethyl- 1 H-indazol-4-y l)-8-fluoro-2-((hexahydro- 1 H- pyrrolizin-7a-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol: A mixture of 1- (7-(5 ,6-dimethy 1- 1 -(tetrahydro-2H-pyran-2-y 1)- 1 H-indazol-4-y l)-8-fluoro-2-((tetrahydro- 1 H- pyrrolizin-7a(5H)-yl)methoxy)pyrido[4,3-d]pyrimidin-4-yl)-3-methylpiperidin-3-ol (290 mg, 460 ⁇ mol) and ACN (0.2 mL) was added HC1dioxane (4 M, 1.98 ⁇ L) in one portion at 25

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