WO2020093639A1 - 一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法 - Google Patents

一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法 Download PDF

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WO2020093639A1
WO2020093639A1 PCT/CN2019/078077 CN2019078077W WO2020093639A1 WO 2020093639 A1 WO2020093639 A1 WO 2020093639A1 CN 2019078077 W CN2019078077 W CN 2019078077W WO 2020093639 A1 WO2020093639 A1 WO 2020093639A1
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phycoerythrin
cds
ratio
shell polymer
magnetic
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PCT/CN2019/078077
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French (fr)
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金辉
桂日军
付永鑫
卜祥宁
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青岛大学
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G77/00—Macromolecular compounds obtained by reactions forming a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon in the main chain of the macromolecule
    • C08G77/04—Polysiloxanes
    • C08G77/22—Polysiloxanes containing silicon bound to organic groups containing atoms other than carbon, hydrogen and oxygen
    • C08G77/26—Polysiloxanes containing silicon bound to organic groups containing atoms other than carbon, hydrogen and oxygen nitrogen-containing groups
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K3/00—Use of inorganic substances as compounding ingredients
    • C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
    • C08K3/20—Oxides; Hydroxides
    • C08K3/22—Oxides; Hydroxides of metals
    • C08K2003/2265—Oxides; Hydroxides of metals of iron
    • C08K2003/2275—Ferroso-ferric oxide (Fe3O4)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K2201/00—Specific properties of additives
    • C08K2201/01—Magnetic additives
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    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2600/00—Assays involving molecular imprinted polymers/polymers created around a molecular template

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  • the invention belongs to the technical field of preparation of nanocomposite materials and molecularly imprinted polymers, and particularly relates to a preparation method of a phycoerythrin ratio fluorescent sensor based on magnetic molecularly imprinted core / shell polymer, and the prepared sensor can be used for phycoerythrin High sensitivity and high selectivity detection.
  • Phycoerythrin is one of the important light-harvesting pigment proteins in seaweed, and it forms a fluorescence family of phycobiliproteins with phycocyanin and allophycocyanin. These proteins are part of the effective energy transfer chain, which guides the light-trapped complex excitation energy to the reaction center containing chlorophyll, and the light energy transfer efficiency can reach 95%.
  • Phycoerythrin is mainly isolated and purified from red algae.
  • Phycoerythrin has strong fluorescence, good light absorption characteristics, high quantum yield, and extensive excitation and emission characteristics in the visible light region.
  • Phycoerythrin can be widely used in diagnostic and bioengineering technologies, such as fluorescence immunoassay, dual-color or multi-color fluorescence analysis, cancer cell surface antigen detection, flow cytometry, antibody fluorescent labeling, biological imaging, food, cosmetics and other fields. Accurate detection of phycoerythrin is still one of the problems that need to be solved urgently. It is of great significance to develop a simple and efficient method for identification and detection of phycoerythrin.
  • molecular imprinting technology can capture highly sensitive and selective molecules in the cavity of polymer molecules in the presence of specific template molecules.
  • the removal of template molecules by elution causes specific sites to remain in the formed polymer network, and the shape and size match the template molecules.
  • molecularly imprinted polymers have the advantages of simple preparation, low cost, good selectivity, high physical strength, and good thermal stability. They have been widely used in chemical / biosensors, chromatographic separation, and solid phase extraction , Drug release and many other important fields.
  • the signal intensity ratio obtained by dual-signal ratio processing has a self-calibration function, which effectively eliminates the interference between the self and the background signal, and improves the accuracy and reliability of the detection results.
  • the present invention reports a novel fluorescence sensor based on magnetic molecularly imprinted core / shell polymer phycoerythrin ratio.
  • B-CDs blue quantum emission carbon quantum dots
  • the silica shell layer is eluted with template molecules to prepare molecularly imprinted polymers SiO 2 -MIPs, that is, Fe 3 O 4 / B-CDs / SiO 2 -MIPs.
  • the magnetic molecularly imprinted core / shell polymer can be used for fluorescence detection of phycoerythrin with high sensitivity and high selectivity ratio.
  • the purpose of the present invention is to overcome the above-mentioned defects in the prior art, design a simple method, low cost, high sensitivity and good selectivity for the preparation of a magnetic molecularly imprinted core / shell polymer-based phycoerythrin ratio fluorescent sensor method.
  • the preparation process of a phycoerythrin ratio fluorescent sensor based on magnetic molecularly imprinted core / shell polymer according to the present invention includes the following steps:
  • a method for preparing a phycoerythrin ratio fluorescent sensor based on magnetic molecularly imprinted core / shell polymer characterized in that the method specifically includes the following steps:
  • B-CDs aminated blue fluorescent emission
  • the prepared dark brown mixed solution was diluted with 20 mL of double-distilled water, centrifuged at 12000 rpm to remove larger particles, the supernatant was collected and filtered with a 0.4 ⁇ m microfiltration membrane, and the filtrate was dialyzed using a dialysis bag with a molecular weight cut-off of 1000 Da Remove unreacted experimental materials, pour out the solution in the dialysis bag, use rotary evaporation to remove 90% of the liquid, and then dry in vacuum to obtain B-CDs, store them at 4 °C in the dark, or disperse in the solution to prepare B-CDs The dispersion was used in subsequent experiments.
  • the product is centrifuged, washed and dried to obtain Fe 3 O 4 / B-CDs / SiO 2 -MIPs.
  • the magnetic molecularly imprinted core / shell polymer is dispersed in the solution to prepare a dispersion for future use.
  • step (1) The aminated blue fluorescent emission B-CDs described in step (1), whose size is 1 to 5 nm;
  • step (2) The carboxylated Fe 3 O 4 magnetic nanoparticles described in step (2), whose size is 10-30 nm;
  • the mass concentration of B-CDs described in step (3) is 1-10 mg mL -1 ; the mass concentration of Fe 3 O 4 magnetic nanoparticles is 5-20 mg mL -1 ; the dosage of phycoerythrin is 0.5-1 ⁇ M;
  • the linear detection range of the molar concentration of phycoerythrin described in step (4) is 1 to 500 nM, and the detection limit is 1 to 10 nanomoles / liter.
  • the effect of the present invention is to report a novel fluorescent sensor for phycoerythrin ratio based on magnetic molecularly imprinted core / shell polymer.
  • Fe 3 O 4 magnetic nanoparticles as the center, blue quantum emission carbon quantum dots (B-CDs) are coupled to the surface, and the template molecules (phycoerythrin) are loaded on the surface of Fe 3 O 4 / B-CDs
  • B-CDs blue quantum emission carbon quantum dots
  • the silica shell layer is eluted with template molecules to prepare molecularly imprinted polymers SiO 2 -MIPs, that is, Fe 3 O 4 / B-CDs / SiO 2 -MIPs magnetic molecularly imprinted core / shell polymers.
  • the method of the invention has simple operation, low cost, easy access to raw materials, strong anti-interference ability of the ratio signal, good accuracy, high sensitivity and selectivity, and can be developed into a novel ratio fluorescence sensor for algae Efficient detection of hemoglobin.
  • FIG. 1 is a schematic diagram of the preparation of a phycoerythrin ratio fluorescent sensor based on magnetic molecularly imprinted core / shell polymer and detection of phycoerythrin according to the present invention
  • FIG. 2 is the fluorescence emission spectrum of the corresponding sensor system measured under different molar concentrations of phycoerythrin using the ratio fluorescence sensor of the present invention
  • Figure 3 is the corresponding phycoerythrin and B-CDs fluorescence emission peak intensity ratio I phycoerythrin / I B-CDs under different molar concentrations of phycoerythrin, fitting the linear relationship between different ratio values and the molar concentration of phycoerythrin .
  • This embodiment relates to a preparation method of a phycoerythrin ratio fluorescent sensor based on magnetic molecularly imprinted core / shell polymer.
  • the preparation process and the principle diagram of the ratio fluorescence detection of phycoerythrin are shown in FIG. 1, and the specific process steps are as follows :
  • the prepared dark brown mixed solution was diluted with 20mL of double-distilled water, centrifuged at 12000rpm to remove larger particles, the supernatant was collected and filtered with a 0.4 ⁇ m microfiltration membrane, and the filtrate was dialyzed with a molecular weight cut-off of 1000Da to remove unreacted
  • the solution in the dialysis bag was poured out, 90% of the liquid was removed by rotary evaporation, and then vacuum-dried to obtain B-CDs, which were stored at 4 ° C in the dark, or dispersed in the solution to prepare a dispersion of B-CDs.
  • the average size of B-CDs was 2 nm.
  • Fe 3 O 4 was obtained, which was stored at 4 ° C in the dark, or dispersed in a solution to prepare a dispersion of Fe 3 O 4 for subsequent experiments.
  • the average size of Fe 3 O 4 was 15 nm.
  • the ratio fluorescence sensor of the present invention is used to measure the fluorescence emission spectrum of the corresponding sensor system at different molar concentrations of phycoerythrin. As shown in FIG. 3, the linear detection range of the phycoerythrin molar concentration obtained based on the ratio fluorescence sensor of the present invention is 5 to 250 nM, and the detection limit is 2 nM.
  • Embodiment 2 The preparation process of the phycoerythrin ratio fluorescent sensor and the principle schematic diagram of the ratio fluorescence detection of phycoerythrin in this embodiment, the preparation process of aminated blue fluorescent emission B-CDs and carboxylated Fe 3 O 4 magnetic nanoparticles
  • the procedure is the same as in Example 1, wherein the average size of B-CDs is 3 nm and the average size of Fe 3 O 4 is 20 nm.
  • Other specific process steps are as follows:
  • Preparation of magnetic molecularly imprinted core / shell polymer 2mL of B-CDs aqueous dispersion is added to 18mL of aqueous dispersion containing 0.8mL Fe 3 O 4 , where the mass concentration of B-CDs is 5mg mL -1 , Fe 3 O 4 The mass concentration of the magnetic nanoparticles is 15mg mL -1 ; after stirring for 30min, add the template molecule phycoerythrin and 20 ⁇ L 3-aminopropyltriethoxysilane, of which the amount of phycoerythrin is 0.8 ⁇ M; continue the reaction for 1h, then Add 40 ⁇ L of ammonia water and 40 ⁇ L of tetraethyl silicate, react in a dark place for 12 h, centrifuge the product, wash three times with a volume ratio of 8: 2 ethanol / acetonitrile, remove the template molecule, the product is centrifuged, washed and dried to obtain
  • phycoerythrin added a certain amount to the polymer dispersion to form a homogeneous mixture. After incubating for 5 minutes in a dark place, measure the fluorescence emission spectrum of the homogeneous mixture at different phycoerythrin concentrations. Fitting the linear relationship between the fluorescence emission peak intensity ratio of phycoerythrin and B-CDs (I phycoerythrin / IB-CDs ) and the molar concentration of phycoerythrin, a phycoerythrin ratio fluorescence sensor was constructed. The linear detection range of phycoerythrin molar concentration is 5 ⁇ 500nM, and the detection limit is 5nM.
  • Example 3 The preparation process of the phycoerythrin ratio fluorescent sensor and the principle diagram of the ratio fluorescence detection of phycoerythrin, the process of preparation of aminated blue fluorescent emission B-CDs and carboxylated Fe 3 O 4 magnetic nanoparticles
  • the procedure is the same as in Example 1, in which the average size of B-CDs is 5 nm and the average size of Fe 3 O 4 is 25 nm.
  • Other specific process steps are as follows:
  • Preparation of magnetic molecularly imprinted core / shell polymer 2mL of B-CDs aqueous dispersion was added to 18mL of aqueous dispersion containing 0.8mL Fe 3 O 4 , where the mass concentration of B-CDs was 8mg mL -1 , Fe 3 O 4 The mass concentration of magnetic nanoparticles is 20mg mL -1 ; after stirring for 30min, add the template molecule phycoerythrin and 20 ⁇ L of 3-aminopropyltriethoxysilane, in which the amount of phycoerythrin is 1 ⁇ M; continue the reaction for 1h, then add 40 ⁇ L ammonia water and 40 ⁇ L tetraethyl silicate were reacted in a dark place for 12h.
  • the product was centrifuged and washed three times with a volume ratio of 8: 2 ethanol / acetonitrile to remove the template molecule.
  • the product was centrifuged, washed and dried to obtain Fe 3 O 4 / B-CDs / SiO 2 -MIPs.
  • the magnetic molecularly imprinted core / shell polymer is dispersed in the solution to prepare a dispersion for future use.
  • phycoerythrin added a certain amount to the polymer dispersion to form a homogeneous mixture. After incubating for 5 minutes in a dark place, measure the fluorescence emission spectrum of the homogeneous mixture at different phycoerythrin concentrations. Fitting the linear relationship between the fluorescence emission peak intensity ratio of phycoerythrin and B-CDs (I phycoerythrin / IB-CDs ) and the molar concentration of phycoerythrin, a phycoerythrin ratio fluorescence sensor was constructed. The linear detection range of phycoerythrin molar concentration is 10 ⁇ 500nM, and the detection limit is 8nM.

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Abstract

本发明具体涉及一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法。以Fe 3O 4磁性纳米粒为中心，表面偶联蓝荧光发射碳量子点B-CDs，在Fe 3O 4/B-CDs表面生长负载了模板分子藻红蛋白的SiO 2壳层，洗脱模板分子制得磁性分子印迹核/壳聚合物Fe 3O 4/B-CDs/SiO 2-MIPs。测定不同藻红蛋白浓度下该聚合物分散液荧光发射光谱，拟合藻红蛋白与B-CDs荧光发射峰强度比率I 藻红蛋白/I B- CDs与藻红蛋白摩尔浓度之间的线性关系，构建藻红蛋白比率荧光传感器。与现有技术相比，本发明方法操作简单，成本低，原料易得，比率信号抗干扰能力强，准确性好，灵敏度和选择性高，可发展成为一种新颖的比率荧光传感器用于藻红蛋白的高效检测。

Description

一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法 技术领域：
本发明属于纳米复合材料与分子印迹聚合物的制备技术领域，具体涉及一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法，其制备的传感器可用于藻红蛋白的高灵敏度和高选择性检测。
背景技术：
当前，海洋表面大规模繁殖的红藻造成的赤潮现象越来越严重，其对人体健康和水生资源的危害引起了广泛的社会关注。藻红蛋白是海藻中重要的捕光色素蛋白之一，其与藻蓝蛋白和别藻蓝蛋白构成了藻胆蛋白的荧光家族。这些蛋白质是有效能量传递链的一部分，它将光捕获的复合物激发能量引导至含有叶绿素的反应中心，光能传递效率可达到95％。藻红蛋白主要从红藻中分离和纯化获得，其具有强荧光，良好的光吸收特性，高量子产率，以及在可见光区域存在广泛的激发和发射等特性。藻红蛋白可广泛用于诊断和生物工程技术领域，如荧光免疫分析、双色或多色荧光分析、癌细胞表面抗原检测、流式细胞技术、抗体荧光标记、生物成像、食品、化妆品等领域。对藻红蛋白进行精确检测仍然是当前亟待解决的问题之一，开发一种简单高效的鉴定和检测藻红蛋白的方法具有重要的意义。
分子印迹技术可在特定模板分子的存在下，将其高灵敏和高选择性地捕获到聚合物分子空穴中。在制备过程中，通过洗脱去除模板分子引 起特定位点保留在形成的聚合物网络中，且形状和大小与模板分子相匹配。分子印迹聚合物的特异性位点和模板分子之间存在匹配关系，可实现对模板分子的高选择性和特异性检测。作为一种特殊的受体形式，分子印迹聚合物具有制备简单、廉价、选择性好、物理强度高、热稳定性好等优点，已被广泛应用于化学/生物传感器、色谱分离、固相萃取、药物释放等诸多重要领域。
经文献检索发现：Munier等从红色食用海藻中采用一步法纯化制得R-藻红蛋白(Mathilde Munier,
Figure PCTCN2019078077-appb-000001
Justine Dumay,Pascal Jaouen,
Figure PCTCN2019078077-appb-000002
Fleurence.One-step purification of R-phycoerythrin from the red edible seaweed Grateloupia turuturu.Journal of Chromatography B,992(2015)23–29.)，其中R-藻红蛋白采用高效液相色谱法检测；Gameiro等对藻红蛋白进行了荧光表征(Carla Gameiro,Andrei B.Utkin,Paulo Cartaxana.Characterisation of estuarine intertidal macroalgae by laser-induced fluorescence.Estuarine,Coastal and Shelf Science 167(2015)119–124)；吴继魁等报道了一种利用R-藻红蛋白荧光来检测DNA杂交的方法(吴继魁,陆云飞,任宁娜,张俊玲.一种利用表面阳离子化的R-藻红蛋白检测DNA杂交的方法.中国发明专利.公开号CN108037103A)；王华林等制备了一种藻红蛋白标记的聚苯乙烯微球用作荧光探针(王华林,张涛,张科登.一种藻红蛋白标记聚苯乙烯微球的荧光探针制备方法.中国发明专利.公开号CN108383936A)。
当前有关藻红蛋白的研究集中在提取、分离、纯化和用作荧光标记探针的研究，对藻红蛋白的分析检测局限于使用高效液相色谱仪和荧光光谱仪进行定量。传统的仪器分析技术普遍存在样品前处理繁琐，操作复杂，耗时费力，灵敏度低，选择性差等问题。在藻红蛋白的检测中， 仅仅依赖单一信号输出，这种检测模式易受背景、试剂、系统和环境条件等因素的影响，导致测定结果波动。相比而言，采用双信号比值处理获得信号的强度比率，具备自校准功能，有效消除了自体和背景信号的干扰，提高了检测结果的准确性和可靠性。基于此，本发明报道了一种新颖的基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器。以Fe 3O 4磁性纳米粒为中心，在其表面偶联蓝荧光发射的碳量子点(B-CDs)，在Fe 3O 4/B-CDs表面生长负载了模板分子(藻红蛋白)的二氧化硅壳层，洗脱模板分子制得分子印迹聚合物SiO 2-MIPs，即制得Fe 3O 4/B-CDs/SiO 2-MIPs。该磁性分子印迹核/壳聚合物可用于高灵敏和高选择性比率荧光检测藻红蛋白。截止目前，尚未有采用基于Fe 3O 4/B-CDs/SiO 2-MIPs磁性分子印迹核/壳聚合物来构建比率荧光传感器，以及采用比率荧光方法来检测藻红蛋白的国内外文献和专利的报道。
发明内容：
本发明的目的在于克服上述现有技术存在的缺陷，设计一种方法简单、成本低廉、灵敏度高、选择性好的一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法。
为了实现上述目的，本发明涉及的一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备工艺包括以下步骤：
1.一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法，其特征在于，该方法具体包括以下步骤：
(1)氨基化蓝荧光发射B-CDs的制备：取0.3mL乙二醚和25mL儿茶酚溶液超声混匀，转入50mL聚四氟乙烯内胆的高压反应釜中，在 180℃下加热反应12h，将制得的深棕色混合液用20mL二次蒸馏水稀释，以12000rpm转速离心除去较大颗粒，收集上清液并采用0.4μm微滤膜过滤，滤液采用截留分子量1000Da透析袋透析处理以清除未反应实验原料，将透析袋中溶液倒出，采用旋转蒸发除去90％的液体，再真空干燥得到B-CDs，在4℃避光条件下储存，或分散在溶液中制备B-CDs的分散液用于后续实验。
(2)羧基化Fe 3O 4磁性纳米粒的制备：向250mL反应瓶中加入摩尔比为2:1的氯化铁和氯化亚铁配成100mL混合溶液，在N 2保护下向反应瓶中加入质量浓度为25％的10mL氨水，快速搅拌反应，用HCl调节溶液pH至碱性，反应10min后加入10mL柠檬酸三钠溶液，将反应瓶放置于80℃的水浴锅中继续搅拌反应30min，产物经离心，洗涤和干燥处理得到Fe 3O 4，在4℃避光条件下储存，或分散在溶液中制备Fe 3O 4的分散液用于后续实验。
(3)磁性分子印迹核/壳聚合物的制备：取2mL B-CDs水分散液加入到18mL含有0.8mL Fe 3O 4的水分散液中，搅拌反应30min后，加入模板分子藻红蛋白和20μL 3-氨基丙基三乙氧基硅烷，继续反应1h，再加入40μL氨水和40μL硅酸四乙酯，在避光处反应12h，将产物离心，用乙醇/乙腈(体积比为8:2)洗涤三次，除去模板分子，产物经离心，洗涤和干燥处理得到Fe 3O 4/B-CDs/SiO 2-MIPs。将此磁性分子印迹核/壳聚合物分散在溶液中制备分散液备用。
(4)在室温和磁力搅拌下，向聚合物分散液中加入一定量的藻红蛋白形成均质混合液，在避光处孵化5min后，测定不同藻红蛋白浓度下均质混合液的荧光发射光谱，拟合藻红蛋白与B-CDs的荧光发射峰强度比率(I 藻红蛋白/I B-CDs)与藻红蛋白摩尔浓度之间的线性关系，构建藻红 蛋白比率荧光传感器。
步骤(1)中所述的氨基化蓝荧光发射B-CDs，其尺寸为1～5nm；
步骤(2)中所述的羧基化Fe 3O 4磁性纳米粒，其尺寸为10～30nm；
步骤(3)中所述的B-CDs质量浓度为1～10mg mL -1；Fe 3O 4磁性纳米粒质量浓度为5～20mg mL -1；藻红蛋白的用量为0.5～1μM；
步骤(4)中所述的藻红蛋白摩尔浓度的线性检测范围为1～500nM，检测极限为1～10纳摩尔/升。
本发明的效果是：报道了一种新颖的基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器。以Fe 3O 4磁性纳米粒为中心，在其表面偶联蓝荧光发射的碳量子点(B-CDs)，在Fe 3O 4/B-CDs表面生长负载了模板分子(藻红蛋白)的二氧化硅壳层，洗脱模板分子制得分子印迹聚合物SiO 2-MIPs，即制得Fe 3O 4/B-CDs/SiO 2-MIPs磁性分子印迹核/壳聚合物。测定不同藻红蛋白浓度下该聚合物均质混合液的荧光发射光谱，拟合藻红蛋白与B-CDs的荧光发射峰强度比率(I 藻红蛋白/I B-CDs)与藻红蛋白摩尔浓度之间的线性关系，构建藻红蛋白比率荧光传感器，用于高灵敏和高选择性比率荧光检测藻红蛋白。与现有技术相比，本发明方法操作简单，成本低，原料易得，比率信号抗干扰能力强，准确性好，灵敏度和选择性高，可发展成为一种新颖的比率荧光传感器用于藻红蛋白的高效检测。
附图说明：
图1为本发明涉及的一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备与藻红蛋白检测的原理示意图；
图2为使用本发明的比率荧光传感器测定不同藻红蛋白摩尔浓度下 对应的传感器体系的荧光发射光谱；
图3为不同藻红蛋白摩尔浓度下对应的藻红蛋白与B-CDs荧光发射峰强度比率I 藻红蛋白/I B-CDs，拟合不同比率值与藻红蛋白摩尔浓度之间的线性关系。
具体实施方式：
下面结合附图并通过具体实施例对本发明进行详细说明。
实施例1：
本实施例涉及的一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法，其制备工艺和比率荧光检测藻红蛋白的原理示意图如图1所示，具体工艺步骤如下：
氨基化蓝荧光发射B-CDs的制备：取0.3mL乙二醚和25mL儿茶酚溶液超声混匀，转入50mL聚四氟乙烯内胆的高压反应釜中，在180℃下加热反应12h，将制得的深棕色混合液用20mL二次蒸馏水稀释，以12000rpm转速离心除去较大颗粒，收集上清液并采用0.4μm微滤膜过滤，滤液采用截留分子量1000Da透析袋透析处理以清除未反应实验原料，将透析袋中溶液倒出，采用旋转蒸发除去90％的液体，再真空干燥得到B-CDs，在4℃避光条件下储存，或分散在溶液中制备B-CDs的分散液用于后续实验，其中B-CDs平均尺寸为2nm。
羧基化Fe 3O 4磁性纳米粒的制备：向250mL反应瓶中加入摩尔比为2:1的氯化铁和氯化亚铁配成100mL混合溶液，在N 2保护下向反应瓶中加入质量浓度为25％的10mL氨水，快速搅拌反应，用HCl调节溶液pH至碱性，反应10min后加入10mL柠檬酸三钠溶液，将反应瓶放置于80℃的水浴锅中继续搅拌反应30min，产物经离心，洗涤和干 燥处理得到Fe 3O 4，在4℃避光条件下储存，或分散在溶液中制备Fe 3O 4的分散液用于后续实验，其中Fe 3O 4平均尺寸为15nm。
磁性分子印迹核/壳聚合物的制备：取2mL B-CDs水分散液加入到18mL含有0.8mL Fe 3O 4的水分散液中，其中B-CDs质量浓度为2mg mL -1，Fe 3O 4磁性纳米粒质量浓度为10mg mL -1；搅拌反应30min后，加入模板分子藻红蛋白和20μL 3-氨基丙基三乙氧基硅烷，其中藻红蛋白用量为0.5μM；继续反应1h，再加入40μL氨水和40μL硅酸四乙酯，在避光处反应12h，将产物离心，用体积比为8:2乙醇/乙腈洗涤三次，除去模板分子，产物经离心，洗涤和干燥处理得到Fe 3O 4/B-CDs/SiO 2-MIPs。将此磁性分子印迹核/壳聚合物分散在溶液中制备分散液备用。
在室温和磁力搅拌下，向聚合物分散液中加入一定量的藻红蛋白形成均质混合液，在避光处孵化5min后，测定不同藻红蛋白浓度下均质混合液的荧光发射光谱，拟合藻红蛋白与B-CDs的荧光发射峰强度比率(I 藻红蛋白/I B-CDs)与藻红蛋白摩尔浓度之间的线性关系，构建藻红蛋白比率荧光传感器。如图2所示，使用本发明的比率荧光传感器测定不同藻红蛋白摩尔浓度下对应的传感器体系荧光发射光谱。如图3所示，基于本发明的比率荧光传感器获得的藻红蛋白摩尔浓度的线性检测范围为5～250nM，检测极限为2nM。
实施例2：本实施例涉及的藻红蛋白比率荧光传感器的制备工艺和比率荧光检测藻红蛋白的原理示意图，氨基化蓝荧光发射B-CDs和羧基化Fe 3O 4磁性纳米粒制备的工艺步骤同实施例1，其中B-CDs平均尺寸为3nm，Fe 3O 4平均尺寸为20nm。其它具体工艺步骤如下：
磁性分子印迹核/壳聚合物的制备：取2mL B-CDs水分散液加入到18mL含有0.8mL Fe 3O 4的水分散液中，其中B-CDs质量浓度为5mg  mL -1，Fe 3O 4磁性纳米粒质量浓度为15mg mL -1；搅拌反应30min后，加入模板分子藻红蛋白和20μL 3-氨基丙基三乙氧基硅烷，其中藻红蛋白用量为0.8μM；继续反应1h，再加入40μL氨水和40μL硅酸四乙酯，在避光处反应12h，将产物离心，用体积比为8:2乙醇/乙腈洗涤三次，除去模板分子，产物经离心，洗涤和干燥处理得到Fe 3O 4/B-CDs/SiO 2-MIPs。将此磁性分子印迹核/壳聚合物分散在溶液中制备分散液备用。
在室温和磁力搅拌下，向聚合物分散液中加入一定量的藻红蛋白形成均质混合液，在避光处孵化5min后，测定不同藻红蛋白浓度下均质混合液的荧光发射光谱，拟合藻红蛋白与B-CDs的荧光发射峰强度比率(I 藻红蛋白/I B-CDs)与藻红蛋白摩尔浓度之间的线性关系，构建藻红蛋白比率荧光传感器。藻红蛋白摩尔浓度的线性检测范围为5～500nM，检测极限为5nM。
实施例3：本实施例涉及的藻红蛋白比率荧光传感器的制备工艺和比率荧光检测藻红蛋白的原理示意图，氨基化蓝荧光发射B-CDs和羧基化Fe 3O 4磁性纳米粒制备的工艺步骤同实施例1，其中B-CDs平均尺寸为5nm，Fe 3O 4平均尺寸为25nm。其它具体工艺步骤如下：
磁性分子印迹核/壳聚合物的制备：取2mL B-CDs水分散液加入到18mL含有0.8mL Fe 3O 4的水分散液中，其中B-CDs质量浓度为8mg mL -1，Fe 3O 4磁性纳米粒质量浓度为20mg mL -1；搅拌反应30min后，加入模板分子藻红蛋白和20μL 3-氨基丙基三乙氧基硅烷，其中藻红蛋白用量为1μM；继续反应1h，再加入40μL氨水和40μL硅酸四乙酯，在避光处反应12h，将产物离心，用体积比为8:2乙醇/乙腈洗涤三次，除去模板分子，产物经离心，洗涤和干燥处理得到Fe 3O 4/B-CDs/SiO 2-MIPs。将此磁性分子印迹核/壳聚合物分散在溶液中制备分散液备用。
在室温和磁力搅拌下，向聚合物分散液中加入一定量的藻红蛋白形成均质混合液，在避光处孵化5min后，测定不同藻红蛋白浓度下均质混合液的荧光发射光谱，拟合藻红蛋白与B-CDs的荧光发射峰强度比率(I 藻红蛋白/I B-CDs)与藻红蛋白摩尔浓度之间的线性关系，构建藻红蛋白比率荧光传感器。藻红蛋白摩尔浓度的线性检测范围为10～500nM，检测极限为8nM。
以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员，在不脱离本发明原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。

Claims (5)

  1. 一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法，其特征在于，该方法具体包括以下步骤：
    (1)氨基化蓝荧光发射B-CDs的制备：取0.3mL乙二醚和25mL儿茶酚溶液超声混匀，转入50mL聚四氟乙烯内胆的高压反应釜中，在180℃下加热反应12h，将制得的深棕色混合液用20mL二次蒸馏水稀释，以12000rpm转速离心除去较大颗粒，收集上清液并采用0.4μm微滤膜过滤，滤液采用截留分子量1000Da透析袋透析处理以清除未反应实验原料，将透析袋中溶液倒出，采用旋转蒸发除去90％的液体，再真空干燥得到B-CDs，在4℃避光条件下储存，或分散在溶液中制备B-CDs的分散液用于后续实验；
    (2)羧基化Fe 3O 4磁性纳米粒的制备：向250mL反应瓶中加入摩尔比为2:1的氯化铁和氯化亚铁配成100mL混合溶液，在N 2保护下向反应瓶中加入质量浓度为25％的10mL氨水，快速搅拌反应，用HCl调节溶液pH至碱性，反应10min后加入10mL柠檬酸三钠溶液，将反应瓶放置于80℃的水浴锅中继续搅拌反应30min，产物经离心，洗涤和干燥处理得到Fe 3O 4，在4℃避光条件下储存，或分散在溶液中制备Fe 3O 4的分散液用于后续实验；
    (3)磁性分子印迹核/壳聚合物的制备：取2mL B-CDs水分散液加入到18mL含有0.8mL Fe 3O 4的水分散液中，搅拌反应30min后，加入模板分子藻红蛋白和20μL 3-氨基丙基三乙氧基硅烷，继续反应1h，再加入40μL氨水和40μL硅酸四乙酯，在避光处反应12h，将产物离心，用乙醇/乙腈(体积比为8:2)洗涤三次，除去模板分子，产物经离心，洗涤和干燥处理得到Fe 3O 4/B-CDs/SiO 2-MIPs，将此磁性分子印迹核/壳聚合物分散在溶液中制备分散液备用；
    (4)在室温和磁力搅拌下，向聚合物分散液中加入一定量的藻红蛋白形成均质混合液，在避光处孵化5min后，测定不同藻红蛋白浓度下均质混合液的荧光发射光谱，拟合藻红蛋白与B-CDs的荧光发射峰强度比率(I 藻红蛋白/I B-CDs)与藻红蛋白摩尔浓度之间的线性关系，构建藻红蛋白比率荧光传感器。
  2. 根据权利要求1所述的一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法,其特征在于，步骤(1)中所述的氨基化蓝荧光发射B-CDs，其尺寸为1～5nm。
  3. 根据权利要求1所述的一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法,其特征在于，步骤(2)中所述的羧基化Fe 3O 4磁性纳米粒，其尺寸为10～30nm。
  4. 根据权利要求1所述的一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法,其特征在于，步骤(3)中所述的B-CDs质量浓度为1～10mg mL -1；Fe 3O 4磁性纳米粒质量浓度为5～20mg mL -1；藻红蛋白的用量为0.5～1μM。
  5. 根据权利要求1所述的一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法,其特征在于，步骤(4)中所述的藻红蛋白摩尔浓度的线性检测范围为1～500nM，检测极限为1～10纳摩尔/升。
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113101903A (zh) * 2021-04-25 2021-07-13 李辉 一种新型复合印迹微球分离纯化京尼平苷酸的方法
CN114518358A (zh) * 2022-02-16 2022-05-20 四川农业大学 农药残留检测用三色传感探针及其制备方法、应用和深度学习的视觉智能监测装置及方法
CN114984926A (zh) * 2022-05-11 2022-09-02 东北电力大学 一种高抗团聚rgo基磁性锂离子印迹聚合物的制备方法
CN115165819A (zh) * 2022-05-27 2022-10-11 天津科技大学 一种快速定量分析芦丁的荧光分子印迹光子晶体凝胶条的制备方法及应用

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109406473B (zh) * 2018-11-08 2019-07-30 青岛大学 一种藻红蛋白比率荧光传感器的制备方法
CN109913200A (zh) * 2019-03-28 2019-06-21 汤新红 一种具有磁性和荧光双重功能的纳米材料及其制备方法
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CN110776910A (zh) * 2019-11-05 2020-02-11 重庆科技学院 双功能磁性荧光纳米复合Fe3O4@CDs微球的制备方法
CN111024673B (zh) * 2020-01-20 2021-08-03 南京医科大学附属逸夫医院 一种比率荧光分子印迹聚合物及其制备方法和应用
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CN113189064B (zh) * 2021-04-22 2022-12-09 西安交通大学 基于糖肽的荧光分子印迹聚合物及制备方法和在糖蛋白筛选和检测中的应用
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CN113945529A (zh) * 2021-10-16 2022-01-18 中南大学 基于双功能可循环磁性荧光传感器的污水中甲卡西酮检测与净化的方法
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CN116120578A (zh) * 2023-02-02 2023-05-16 江苏大学 一种基于CDs@PCN-224纳米复合材料构建的分子印迹光电化学传感器及其用途
CN116396496A (zh) * 2023-05-19 2023-07-07 安徽建筑大学 一种分子印迹Zr-MOF荧光探针材料及其制备方法和应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104277176A (zh) * 2013-07-10 2015-01-14 中国科学院烟台海岸带研究所 荧光蛋白印迹磁性复合微球的制备方法
CN106540668A (zh) * 2015-09-18 2017-03-29 中华人民共和国淮安出入境检验检疫局 磁性亲水分子印迹复合材料及其制备方法
CN106833646A (zh) * 2016-12-31 2017-06-13 浙江工业大学 一种基于离子印迹的比率荧光探针及其制备与应用
CN108414740A (zh) * 2018-01-16 2018-08-17 湖北新纵科病毒疾病工程技术有限公司 一种荧光-磁性双功能微球及其制备方法
CN109406473A (zh) * 2018-11-08 2019-03-01 青岛大学 一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7598087B2 (en) * 2004-01-07 2009-10-06 The Research Foundation Of State University Of New York Protein imprinted polymers with integrated emission sites
CN102012358B (zh) * 2010-10-15 2013-05-15 济南大学 现场快速多组分检测痕量食品添加剂的量子点分子印迹微球石英荧光传感器的研究与应用
CN103739846B (zh) * 2013-12-27 2016-04-06 江苏大学 一种量子点荧光印迹聚合物的制备方法
CN104075925B (zh) * 2014-07-22 2016-08-17 中国科学院烟台海岸带研究所 一种基于量子点的蛋白质核壳印迹微球的制备方法
CN104356323B (zh) * 2014-10-13 2017-05-17 北京大学 一种磁性分子印迹纳米颗粒及其制备方法和应用
CN104926981B (zh) * 2015-05-07 2017-06-16 天津科技大学 一种基于分子印迹‑碳点荧光传感材料的制备方法
CN105385438A (zh) * 2015-11-30 2016-03-09 江苏大学 一种氨基碳量子点荧光硅基印迹传感器的制备方法
US20170355797A1 (en) * 2016-06-14 2017-12-14 University Of New Hampshire Molecularly imprinted copolymer compounds and methods of preparation and use thereof
CN106442436B (zh) * 2016-07-12 2019-11-05 江苏大学 用于检测水中痕量4-硝基苯酚的磁性量子点印迹材料、制备方法及用途
CN106084232B (zh) * 2016-07-27 2019-06-28 齐齐哈尔大学 荧光磁性氧化石墨烯基4-氯酚分子印迹聚合物的制备和应用
CN106198478B (zh) * 2016-08-03 2018-12-07 陕西师范大学 基于量子点比率荧光的分子印迹聚合物检测米托蒽醌的方法
CN107421937B (zh) * 2017-08-29 2019-11-05 浙江工业大学 荧光共振能量转移生物传感器的组胺检测方法
CN108037103B (zh) 2017-12-04 2020-04-28 上海海洋大学 一种利用表面阳离子化的r-藻红蛋白检测dna杂交的方法
CN108383936A (zh) 2018-01-16 2018-08-10 湖北新纵科病毒疾病工程技术有限公司 一种藻红蛋白标记聚苯乙烯微球的荧光探针制备方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104277176A (zh) * 2013-07-10 2015-01-14 中国科学院烟台海岸带研究所 荧光蛋白印迹磁性复合微球的制备方法
CN106540668A (zh) * 2015-09-18 2017-03-29 中华人民共和国淮安出入境检验检疫局 磁性亲水分子印迹复合材料及其制备方法
CN106833646A (zh) * 2016-12-31 2017-06-13 浙江工业大学 一种基于离子印迹的比率荧光探针及其制备与应用
CN108414740A (zh) * 2018-01-16 2018-08-17 湖北新纵科病毒疾病工程技术有限公司 一种荧光-磁性双功能微球及其制备方法
CN109406473A (zh) * 2018-11-08 2019-03-01 青岛大学 一种基于磁性分子印迹核/壳聚合物的藻红蛋白比率荧光传感器的制备方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HE, WEIJIE ET AL.: "Ratiometric Fluorescence and Visual Imaging Detection of Dopamine Based on Carbon Dots/Copper Nanoclusters Dual-emitting Nanohybrids", TALANTA, vol. 178, 9 September 2017 (2017-09-09), pages 109 - 115, XP085275324, DOI: 20190702101509A *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113101903A (zh) * 2021-04-25 2021-07-13 李辉 一种新型复合印迹微球分离纯化京尼平苷酸的方法
CN114518358A (zh) * 2022-02-16 2022-05-20 四川农业大学 农药残留检测用三色传感探针及其制备方法、应用和深度学习的视觉智能监测装置及方法
CN114518358B (zh) * 2022-02-16 2023-10-27 四川农业大学 农药残留检测用三色传感探针及其制备方法、应用和深度学习的视觉智能监测装置及方法
CN114984926A (zh) * 2022-05-11 2022-09-02 东北电力大学 一种高抗团聚rgo基磁性锂离子印迹聚合物的制备方法
CN115165819A (zh) * 2022-05-27 2022-10-11 天津科技大学 一种快速定量分析芦丁的荧光分子印迹光子晶体凝胶条的制备方法及应用

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