WO2012155295A8 - 一种工业化生产玉米降压活性肽的方法 - Google Patents

一种工业化生产玉米降压活性肽的方法 Download PDF

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Publication number
WO2012155295A8
WO2012155295A8 PCT/CN2011/000862 CN2011000862W WO2012155295A8 WO 2012155295 A8 WO2012155295 A8 WO 2012155295A8 CN 2011000862 W CN2011000862 W CN 2011000862W WO 2012155295 A8 WO2012155295 A8 WO 2012155295A8
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WIPO (PCT)
Prior art keywords
corn
production method
industrial production
antihypertensive peptides
antihypertensive
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PCT/CN2011/000862
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English (en)
French (fr)
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WO2012155295A1 (zh
Inventor
蔡木易
谷瑞增
易维学
鲁军
马勇
董哲
徐亚光
潘兴昌
马永庆
林峰
金振涛
陈亮
陆路
刘文颖
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中国食品发酵工业研究院
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Priority to PCT/CN2011/000862 priority Critical patent/WO2012155295A1/zh
Priority to JP2013536982A priority patent/JP5763203B2/ja
Priority to CN201180034661.0A priority patent/CN103052717B/zh
Publication of WO2012155295A1 publication Critical patent/WO2012155295A1/zh
Publication of WO2012155295A8 publication Critical patent/WO2012155295A8/zh
Priority to US13/894,379 priority patent/US8940685B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/01—Hydrolysed proteins; Derivatives thereof
    • A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/12—Antihypertensives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06026—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atom, i.e. Gly or Ala

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medical Informatics (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Mycology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

本发明公开一种以玉米胚芽蛋白为原料工业化生产降压活性肽的方法，属于食品营养和生物技术领域。本发明采用Alcalase2.4L以及木瓜蛋白酶和Dispase ll对玉米胚芽蛋白连续酶解，然后通过离心、超滤、浓缩和喷雾干燥等工序，制得玉米降压活性肽。所获降压活性肽中分子量小于1000 Da的组分占92%以上，肽段丙氨酸-酪氨酸（Ala-Tyr，AY）在0.6%以上。经检测表明，玉米降压活性肽具有良好的体外ACE抑制活性以及对温度、PH值和主要胃肠消化酶的稳定性，并且对原发性高血压大鼠具有明显的降血压作用。本发明的生产方法具有生物活性高、加工成本低、处理量大、便于工业化生产的优点。以此方法制备的玉米降压活性肽可以作为新的功能性营养成分应用于食品、保健食品和药品的开发与生产，具有广阔的市场前景。
PCT/CN2011/000862 2011-05-17 2011-05-17 一种工业化生产玉米降压活性肽的方法 WO2012155295A1 (zh)

Priority Applications (4)

Application Number Priority Date Filing Date Title
PCT/CN2011/000862 WO2012155295A1 (zh) 2011-05-17 2011-05-17 一种工业化生产玉米降压活性肽的方法
JP2013536982A JP5763203B2 (ja) 2011-05-17 2011-05-17 トウモロコシ降圧活性ペプチドを調製するための工業的方法
CN201180034661.0A CN103052717B (zh) 2011-05-17 2011-05-17 一种工业化生产玉米降压活性肽的方法
US13/894,379 US8940685B2 (en) 2011-05-17 2013-05-14 Method for preparing active peptides from corn germ proteins

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Application Number Priority Date Filing Date Title
PCT/CN2011/000862 WO2012155295A1 (zh) 2011-05-17 2011-05-17 一种工业化生产玉米降压活性肽的方法

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US13/894,379 Continuation-In-Part US8940685B2 (en) 2011-05-17 2013-05-14 Method for preparing active peptides from corn germ proteins

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WO2012155295A1 WO2012155295A1 (zh) 2012-11-22
WO2012155295A8 true WO2012155295A8 (zh) 2013-05-10

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US (1) US8940685B2 (zh)
JP (1) JP5763203B2 (zh)
CN (1) CN103052717B (zh)
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CN112592949A (zh) * 2020-12-22 2021-04-02 赵素英 一种葵花盘小分子肽的制备方法
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CN113061633A (zh) * 2021-03-08 2021-07-02 汕尾市五丰海洋生物科技有限公司 一种新型活性肽及其制备工艺
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Also Published As

Publication number Publication date
JP5763203B2 (ja) 2015-08-12
CN103052717B (zh) 2015-01-14
US8940685B2 (en) 2015-01-27
CN103052717A (zh) 2013-04-17
US20130252877A1 (en) 2013-09-26
WO2012155295A1 (zh) 2012-11-22
JP2014500005A (ja) 2014-01-09

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