WO2012145725A2 - Oscillating amplification reaction for nucleic acids - Google Patents

Oscillating amplification reaction for nucleic acids Download PDF

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Publication number
WO2012145725A2
WO2012145725A2 PCT/US2012/034589 US2012034589W WO2012145725A2 WO 2012145725 A2 WO2012145725 A2 WO 2012145725A2 US 2012034589 W US2012034589 W US 2012034589W WO 2012145725 A2 WO2012145725 A2 WO 2012145725A2
Authority
WO
WIPO (PCT)
Prior art keywords
temperature
nucleic acid
dna polymerase
primer
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/034589
Other languages
English (en)
French (fr)
Other versions
WO2012145725A3 (en
Inventor
Hong Cai
Nathan J. COBB
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mesa Tech International Inc
Original Assignee
Mesa Tech International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47042196&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2012145725(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to JP2014506607A priority Critical patent/JP5731066B2/ja
Priority to KR1020167005417A priority patent/KR101878889B1/ko
Priority to KR1020137030853A priority patent/KR20140044323A/ko
Priority to EP12773717.9A priority patent/EP2699698B9/en
Priority to CN201280030581.2A priority patent/CN103608467B/zh
Priority to DK12773717.9T priority patent/DK2699698T4/da
Priority to US14/113,200 priority patent/US9428781B2/en
Application filed by Mesa Tech International Inc filed Critical Mesa Tech International Inc
Priority to CA2856304A priority patent/CA2856304C/en
Priority to ES12773717T priority patent/ES2621390T5/es
Publication of WO2012145725A2 publication Critical patent/WO2012145725A2/en
Publication of WO2012145725A3 publication Critical patent/WO2012145725A3/en
Anticipated expiration legal-status Critical
Priority to US15/247,728 priority patent/US10316358B2/en
Priority to US16/377,749 priority patent/US11293058B2/en
Priority to US17/696,027 priority patent/US20220205019A1/en
Priority to US18/656,534 priority patent/US12421543B2/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature

Definitions

  • Yet another embodiment of the present invention provides a method for amplifying a template of nucleic acid target sequence contained in a sample.
  • the method includes contacting the sample with an amplification reaction mixture comprising a primer or a primer pair having a length of between 35-70 base pairs and complementary to the template of the nucleic acid target sequence and wherein the melting temperature of each primer of the primer pair is between 70- 80°C.
  • the amplification reaction mixture also includes DMSO, monovalent cation, divalent cation, dNTPs, and DNA Polymerase.
  • Another embodiment of the present invention provides an amplification reaction mixture buffer comprising one or more of the following: single strand or double strand nucleic acid destabilizer; monovalent cation; divalent cation; dNTPs ;and DNA Polymerase buffered at a pH to support activity.
  • the DNA polymerase may be a thermostable DNA polymerase.
  • the DNA polymerase may be selected from the group consisting of TAQ DNA polymerase, VentR DNA polymerase, and DeepVentR DNA polymerase but not limited thereto.
  • the DNA polymerase may have a strand displacing activity.
  • the DNA polymerase may be selected which does not have 3'-> 5' exonuclease activity.
  • Nucleic acid samples may be isolated from cells or viruses and may include chromosomal DNA, extra-chromosomal DNA including plasmid DNA, recombinant DNA, DNA fragments, messenger RNA, ribosomal RNA, transfer RNA, double stranded RNA or other RNAs that occur in cells or viruses.
  • Nucleic acid may be isolated from any number of sources such as agriculture, food, environmental, fermentations, or biological fluids such as saliva, blood, nasal or lung aspirates, cerebrospinal fluid, sputum, stool, milk, swabs of mucosal tissues, tissue samples, or cells.
  • Nucleic acid may be isolated, cloned or synthesized in vitro. Within the described nucleic acids above, individual nucleotides may be subject to modification or chemical alterations such as methylation. These modifications or alterations may arise naturally or by in vitro synthesis.
  • a method for exponentially amplifying a specific nucleic acid target by thermal cycling where temperature variation is preferably less than 20 °C, more preferably less than 15 °C, and even more preferably less than 10 °C. This includes the steps of providing a single-stranded template of the nucleic acid to be amplified,
  • oligonucleotide primer pair may be used to simultaneously amplify multiple nucleic acid targets in the same reaction mixture.
  • the oligonucleotide primers may have a length and GC content so that the melting temperature is greater than 65 °C under universally accepted PCR buffer conditions, preferably greater than 70 °C.
  • the DNA polymerase used is preferably selected from Taq DNA polymerase, VentR DNA polymerase, DeepVentR DNA polymerase, and similar thermostable DNA polymerases.
  • the DNA polymerase possesses a strand- displacing activity and does not contain a 3' ⁇ 5' exonuclease activity (see figure 1 B).
  • Platinum Taq is a commercially available hot-start enzyme (Invitrogen, Carlsbad, CA) that is conjugated to an antibody which dissociates upon heating the reaction solution to 94 °C under normal PCR conditions. In the presence of template nucleic acid, this primer pair will produce a product of 153 bp. It is clear that this hot-start preparation is no better than conventional Taq in reducing the formation of ⁇ 1 10 bp primer dimmers during OPCRar. One complication of long primers used in the OPCRar reaction is that they are more prone to result in non-specific and unwanted amplification products known as primer dimmers.
  • OPCRar according to one embodiment of the present invention was performed in the presence or absence of thermostable SSB under the following conditions: / ' ) 1 5% DMSO; / ' / ' ) 1 5% DMSO, 5% Glycerol; Hi) 15% DMSO, 0.25 M Betaine.
  • the cycling parameters used for all reactions were 75 °C for 1 5 sec and 65 °C for 15 sec, repeated 45 times.
  • PCR cyclers and methods have controlled heating design where ramping up is about > 3 deg/second, and ramping down rate is about >1 deg/second.
  • Ramping time is not included in the typical cycling profiles, the instrument does not start counting time duration during denaturation, annealing and extension stage until the desired temperature is reached. For instance, denaturation time is 10 second at 90 deg, the instrument will not starting counting the 10 second time until 90 deg is reached.
  • a system and method of the present invention provides for a low cost heater device which is designed for example the 80 deg for 10 second means the time starts counting when the heating process starts (rather than waiting until the desired denaturing temperature is reached).
  • TYPE Bacterial DNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
PCT/US2012/034589 2011-04-20 2012-04-20 Oscillating amplification reaction for nucleic acids Ceased WO2012145725A2 (en)

Priority Applications (13)

Application Number Priority Date Filing Date Title
ES12773717T ES2621390T5 (es) 2011-04-20 2012-04-20 Reacción de amplificación oscilante para ácidos nucleicos
CA2856304A CA2856304C (en) 2011-04-20 2012-04-20 Oscillating amplification reaction for nucleic acids
KR1020137030853A KR20140044323A (ko) 2011-04-20 2012-04-20 핵산의 왕복 증폭 반응
EP12773717.9A EP2699698B9 (en) 2011-04-20 2012-04-20 Oscillating amplification reaction for nucleic acids
CN201280030581.2A CN103608467B (zh) 2011-04-20 2012-04-20 用于核酸的振荡扩增反应
DK12773717.9T DK2699698T4 (da) 2011-04-20 2012-04-20 Oscillerende amplifikations-reaktion for nukleinsyrer
US14/113,200 US9428781B2 (en) 2011-04-20 2012-04-20 Oscillating amplification reaction for nucleic acids
JP2014506607A JP5731066B2 (ja) 2011-04-20 2012-04-20 核酸のための周期変動性増幅反応
KR1020167005417A KR101878889B1 (ko) 2011-04-20 2012-04-20 핵산의 왕복 증폭 반응
US15/247,728 US10316358B2 (en) 2011-04-20 2016-08-25 Oscillating amplification reaction for nucleic acids
US16/377,749 US11293058B2 (en) 2011-04-20 2019-04-08 Oscillating amplification reaction for nucleic acids
US17/696,027 US20220205019A1 (en) 2011-04-20 2022-03-16 Oscillating Amplification Reaction For Nucleic Acids
US18/656,534 US12421543B2 (en) 2011-04-20 2024-05-06 Oscillating amplification reaction for nucleic acids

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161477437P 2011-04-20 2011-04-20
US201161477357P 2011-04-20 2011-04-20
US61/477,357 2011-04-20
US61/477,437 2011-04-20

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US14/113,200 A-371-Of-International US9428781B2 (en) 2011-04-20 2012-04-20 Oscillating amplification reaction for nucleic acids
US15/247,728 Continuation US10316358B2 (en) 2011-04-20 2016-08-25 Oscillating amplification reaction for nucleic acids

Publications (2)

Publication Number Publication Date
WO2012145725A2 true WO2012145725A2 (en) 2012-10-26
WO2012145725A3 WO2012145725A3 (en) 2013-03-14

Family

ID=47042196

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2012/034589 Ceased WO2012145725A2 (en) 2011-04-20 2012-04-20 Oscillating amplification reaction for nucleic acids
PCT/US2012/034596 Ceased WO2012145730A2 (en) 2011-04-20 2012-04-20 Integrated device for nucleic acid detection and identification

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2012/034596 Ceased WO2012145730A2 (en) 2011-04-20 2012-04-20 Integrated device for nucleic acid detection and identification

Country Status (10)

Country Link
US (9) US10519492B2 (https=)
EP (2) EP2699698B9 (https=)
JP (3) JP5731066B2 (https=)
KR (3) KR101508670B1 (https=)
CN (2) CN103649333B (https=)
CA (2) CA2856304C (https=)
DK (2) DK2699698T4 (https=)
ES (2) ES2583135T3 (https=)
PT (1) PT2699698T (https=)
WO (2) WO2012145725A2 (https=)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3055430A4 (en) * 2013-10-09 2017-06-21 Fluoresentric, Inc. Multiplex probes
US10337056B2 (en) 2007-03-28 2019-07-02 Fluoresentric, Inc. Dynamic flux nucleic acid sequence amplification
US10458978B2 (en) 2006-08-22 2019-10-29 Triad National Security, Llc Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US10519492B2 (en) 2011-04-20 2019-12-31 Mesa Biotech, Inc. Integrated device for nucleic acid detection and identification
WO2020035698A1 (en) * 2018-08-17 2020-02-20 Touchlight IP Limited Synthesis of dna with improved yield
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
US11572580B2 (en) 2017-06-07 2023-02-07 Takara Bio Inc. Oligonucleotide preservation method
WO2023159127A1 (en) * 2022-02-17 2023-08-24 One Lambda, Inc. Systems and methods for protein detection
WO2024083473A1 (de) * 2022-10-19 2024-04-25 Robert Bosch Gmbh Verfahren und vorrichtung zur voramplifikation eines nukleinsäuregemisches
US12023672B2 (en) 2015-04-24 2024-07-02 Mesa Biotech, Inc. Fluidic test cassette
EP4437124A4 (en) * 2021-11-24 2025-10-08 D Nome Private Ltd RAPID METHOD FOR ISOTHERMAL AMPLIFICATION MEDIATED BY REVERSE TRANSCRIPTION LOOP-LAMPS AT ROOM TEMPERATURE (RT-LAMP) AND REAGENT KIT

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8837159B1 (en) * 2009-10-28 2014-09-16 Amazon Technologies, Inc. Low-profile circuit board assembly
US9758837B2 (en) * 2011-08-02 2017-09-12 The United States Of America, As Represented By The Secretary Of Agriculture Sensitive and rapid method for Candidatus Liberibacter species detection
US9121046B2 (en) * 2011-11-16 2015-09-01 New England Biolabs, Inc. Reducing template independent primer extension and threshold time for loop mediated isothermal amplification
WO2015017413A1 (en) * 2013-07-29 2015-02-05 Mbio Diagnostics, Inc. Assay cartridge processing systems and methods and associated assay cartridges
US10195610B2 (en) 2014-03-10 2019-02-05 Click Diagnostics, Inc. Cartridge-based thermocycler
KR101809645B1 (ko) 2014-05-30 2017-12-15 주식회사 이지다이아텍 자동 면역분석 수행장치
MX372869B (es) 2014-06-18 2020-07-07 Zoetis Denmark Aps Un sistema de detección microfluídico y un cartucho microfluídico.
EP3168287A4 (en) 2014-07-08 2018-01-24 National Institute of Advanced Industrial Science and Technology Nucleic acid amplification device, nucleic acid amplification method, and chip for nucleic acid amplification
DE102014015586B3 (de) * 2014-10-21 2016-03-31 Webasto SE Heizgerät
EP4029606A1 (en) 2014-12-31 2022-07-20 Visby Medical, Inc. Molecular diagnostic testing
US9671303B2 (en) * 2015-03-10 2017-06-06 Ford Global Technologies, Llc Method and system for laser pressure transducer
DE102015110092B4 (de) * 2015-06-23 2017-08-10 Endress + Hauser Gmbh + Co. Kg Feldgerät zum Einsatz in hygienischen Anwendungen in der Prozess- und Automatisierungstechnik und Verfahren zu dessen Herstellung
US10775370B2 (en) 2015-07-17 2020-09-15 Stat-Diagnostica & Innovation, S.L. Fluidic system for performing assays
US10040069B2 (en) 2015-07-23 2018-08-07 General Electric Company Amplification and detection of nucleic acids
ES2927062T3 (es) * 2016-03-14 2022-11-02 Lucira Health Inc Dispositivos y procedimientos para modificar propiedades ópticas
US10987674B2 (en) 2016-04-22 2021-04-27 Visby Medical, Inc. Printed circuit board heater for an amplification module
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
USD800331S1 (en) 2016-06-29 2017-10-17 Click Diagnostics, Inc. Molecular diagnostic device
MX2018015889A (es) 2016-06-29 2019-05-27 Click Diagnostics Inc Dispositivos y metodos para la deteccion de moleculas usando una celda de flujo.
AU2017290753B2 (en) * 2016-06-30 2021-12-09 Visby Medical, Inc. Devices and methods for nucleic acid extraction
USD800913S1 (en) 2016-06-30 2017-10-24 Click Diagnostics, Inc. Detection window for molecular diagnostic device
USD800914S1 (en) 2016-06-30 2017-10-24 Click Diagnostics, Inc. Status indicator for molecular diagnostic device
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
JP2018023360A (ja) * 2016-07-29 2018-02-15 セイコーエプソン株式会社 核酸増幅反応方法、試薬、および試薬の使用方法
CN108431603B (zh) 2016-12-28 2020-04-21 纽勤有限公司 流体限位器筒式组件及其使用方法
KR101997097B1 (ko) 2016-12-30 2019-07-05 주식회사 이지다이아텍 거대 자성입자 복합체를 이용한 자동 면역분석장치 및 방법
USD834721S1 (en) 2017-03-03 2018-11-27 Neogen Corporation Assay cartridge
KR20190032810A (ko) 2017-09-20 2019-03-28 주식회사 이지다이아텍 자성 면역분석장치 및 방법
EP3459632A1 (en) * 2017-09-26 2019-03-27 Lunaphore Technologies SA Microfluidic cartrige with built-in sampling device
SG11202002931VA (en) 2017-11-09 2020-04-29 Visby Medical Inc Portable molecular diagnostic device and methods for the detection of target viruses
GB2569965A (en) * 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
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GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
KR20210081335A (ko) 2018-09-03 2021-07-01 비스비 메디컬, 인코포레이티드 항생제 감수성 시험을 위한 장치 및 방법
WO2020079274A1 (en) * 2018-10-19 2020-04-23 Univercells S.A. Method for decontaminating a biomolecule production system and a system suitable for decontamination
US12390812B2 (en) 2018-12-19 2025-08-19 Nuclein, Llc Apparatus and methods for molecular diagnostics
JP2022530323A (ja) 2019-04-28 2022-06-29 ビスビュー メディカル，インコーポレイテッド デジタル検出能力および無線接続性を有する分子診断デバイス
US11352675B2 (en) 2020-01-03 2022-06-07 Visby Medical, Inc. Devices and methods for antibiotic susceptability testing
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WO2021147910A1 (en) * 2020-01-21 2021-07-29 Qingdao Navid Biotechnology Co., Ltd. Methods and kits for amplification and detection of nucleic acids
CN113215224A (zh) * 2020-01-21 2021-08-06 青岛大学 扩增和检测核酸的方法及试剂盒
US20230143118A1 (en) * 2020-06-15 2023-05-11 Arizona Board Of Regents On Behalf Of Arizona State University Point of need diagnostic with thermal control
JP2023539299A (ja) * 2020-08-26 2023-09-13 センス バイオディテクション リミテッド デバイス
WO2022043697A1 (en) * 2020-08-26 2022-03-03 Sense Biodetection Limited Devices
USD1064314S1 (en) 2021-08-13 2025-02-25 Visby Medical, Inc. Molecular diagnostic device
WO2023023638A1 (en) 2021-08-20 2023-02-23 Singular Genomics Systems, Inc. Chemical and thermal assisted nucleic acid amplification methods
US20240102085A1 (en) * 2022-02-15 2024-03-28 Cepheid EXPONENTIAL BASE-GREATER-THAN-BASE 2 NUCLEIC ACID AMPLIFICATION USING HIGH- AND LOW-Tm PRIMERS
WO2023196973A1 (en) * 2022-04-08 2023-10-12 The Broad Institute, Inc. Amplification assays using crispr-cas based detection
AU2023263416A1 (en) 2022-04-29 2024-11-07 Cepheid Nucleic acid extraction and isolation with heat labile silanes and chemically modified solid supports
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US12582985B2 (en) 2022-08-02 2026-03-24 City University Of Hong Kong Sample-in-result-out closed microfluidic device for nucleic acid molecular point-of-care testing detection
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
WO1994023055A1 (en) 1993-03-30 1994-10-13 United States Biochemical Corporation Cycle sequencing with non-thermostable dna polymerases
US6214587B1 (en) 1994-03-16 2001-04-10 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification

Family Cites Families (140)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3667607A (en) 1970-11-27 1972-06-06 Baker Chem Co J T Chromatographic material
US4235601A (en) 1979-01-12 1980-11-25 Thyroid Diagnostics, Inc. Test device and method for its use
US4663277A (en) 1983-05-20 1987-05-05 Profile Diagnostic Sciences Inc. Virus detection method and materials
US4683105A (en) 1985-10-31 1987-07-28 Westinghouse Electric Corp. Testable, fault-tolerant power interface circuit for normally de-energized loads
US4960691A (en) 1986-09-29 1990-10-02 Abbott Laboratories Chromatographic test strip for determining ligands or receptors
IE940110L (en) 1989-03-23 1990-09-23 Bunce Roger A Liquid transfer devices
US5541099A (en) 1989-08-10 1996-07-30 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity
US5225163A (en) 1989-08-18 1993-07-06 Angenics, Inc. Reaction apparatus employing gravitational flow
ES2116977T3 (es) 1990-05-11 1998-08-01 Microprobe Corp Soportes solidos para ensayos de hibridacion de acidos nucleicos y metodos para inmovilizar oligonucleotidos de modo covalente.
US6007999A (en) 1991-07-31 1999-12-28 Idexx Laboratories, Inc. Reversible flow chromatographic binding assay
GB9123922D0 (en) 1991-11-11 1992-01-02 Bunce Roger A Liquid transfer devices
JP2832117B2 (ja) 1991-11-29 1998-12-02 キヤノン株式会社 サンプル測定デバイス及びサンプル測定システム
WO1993014217A1 (en) 1992-01-10 1993-07-22 Life Technologies, Inc. Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
US6555349B1 (en) 1993-01-22 2003-04-29 Cornell Research Foundation, Inc. Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
US5516664A (en) 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
GB9307319D0 (en) 1993-04-07 1993-06-02 British Tech Group Liquid transfer devices
US6037127A (en) 1994-03-31 2000-03-14 E. I. Du Pont De Nemours And Company Method for detection of non-denatured nucleic acid fragments
GB9416002D0 (en) 1994-08-08 1994-09-28 Univ Cranfield Fluid transport device
GB9502112D0 (en) 1995-02-03 1995-03-22 British Biocell Int Assay device and method
US20040110167A1 (en) 1995-07-13 2004-06-10 Gerdes John C. Lateral flow system for nucleic acid detection
US5989813A (en) 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
DE69726404T2 (de) 1996-01-05 2004-09-09 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mesothelinantigen, verfahren und testsatz zur targetierung
US6750031B1 (en) 1996-01-11 2004-06-15 The United States Of America As Represented By The Secretary Of The Navy Displacement assay on a porous membrane
EP0880598A4 (en) 1996-01-23 2005-02-23 Affymetrix Inc RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM
US5741647A (en) 1996-02-16 1998-04-21 Tam; Joseph Wing On Flow through nucleic acid hybridisation uses thereof and a device thereof
DE19609838A1 (de) 1996-03-13 1997-09-18 Boehringer Mannheim Gmbh Verfahren und Teststreifen zur Bestimmung eines Analyten
US5824478A (en) 1996-04-30 1998-10-20 Vysis, Inc. Diagnostic methods and probes
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
EP0946749A1 (en) 1996-11-20 1999-10-06 The Regents Of The University Of Michigan Microfabricated isothermal nucleic acid amplification devices and methods
US5922617A (en) 1997-11-12 1999-07-13 Functional Genetics, Inc. Rapid screening assay methods and devices
US6190612B1 (en) 1998-01-21 2001-02-20 Bayer Corporation Oxygen sensing membranes and methods of making same
ATE328278T1 (de) 1998-03-30 2006-06-15 Orasure Technologies Inc Sammelapparat für einschrittigen test von mundflüssigkeiten
US7640083B2 (en) 2002-11-22 2009-12-29 Monroe David A Record and playback system for aircraft
US6207379B1 (en) 1998-09-11 2001-03-27 One Lambda Method for amplification of DNA
US6261779B1 (en) 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
AU1821700A (en) 1998-11-18 2000-06-05 Orchid Biosciences, Inc. One-step nucleic acid dipstick device with movable membrane
WO2000031538A1 (en) 1998-11-23 2000-06-02 Praxsys Biosystems, Inc. Improved lateral flow assays
US6416642B1 (en) 1999-01-21 2002-07-09 Caliper Technologies Corp. Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection
US20020177135A1 (en) 1999-07-27 2002-11-28 Doung Hau H. Devices and methods for biochip multiplexing
US6300069B1 (en) 1999-05-03 2001-10-09 Qiagen Gmbh Generation and amplification of nucleic acids from ribonucleic acids
US6743399B1 (en) 1999-10-08 2004-06-01 Micronics, Inc. Pumpless microfluidics
US6471916B1 (en) 1999-11-09 2002-10-29 Packard Instrument Company Apparatus and method for calibration of a microarray scanning system
US6875619B2 (en) 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
US20020058252A1 (en) 1999-12-06 2002-05-16 Ananiev Evgueni V. Short shared nucleotide sequences
US6468749B1 (en) 2000-03-30 2002-10-22 Quark Biotech, Inc. Sequence-dependent gene sorting techniques
GB0016813D0 (en) 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (4)
GB0016814D0 (en) 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (3)
WO2002022884A2 (en) 2000-09-12 2002-03-21 Transgenetics Incorporated Microarrayed organization of transcription factor target genes
EP1328342A4 (en) 2000-10-10 2006-03-15 Aviva Biosciences Corp INTEGRATED BIOCHIP SYSTEM FOR SAMPLE PREPARATION AND ANALYSIS
EP1347299B1 (en) 2000-12-26 2006-03-08 Matsushita Electric Industrial Co., Ltd. Specific bonding analysis method and specific bonding analysis device using it
WO2002072773A2 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
JP4542312B2 (ja) 2001-03-09 2010-09-15 ニューゲン テクノロジーズ， インコーポレイテッド Ｒｎａ配列の増幅のための方法および組成物
AU2002307152A1 (en) 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US7105311B2 (en) 2001-05-03 2006-09-12 Immunetics, Inc. Systems and methods for detection of analytes in biological fluids
ATE490267T1 (de) 2001-06-05 2010-12-15 Curevac Gmbh Stabilisierte mrna mit erhöhtem g/c-gehalt, kodierend für ein virales antigen
US20030044862A1 (en) 2001-06-22 2003-03-06 The Board Of Trustees Of The Leland Stanford Jr. University Diagnostic marker for tumor hypoxia and prognosis
WO2003014740A1 (en) 2001-08-09 2003-02-20 Matsushita Electric Industrial Co., Ltd. Biosensors and measurement method
US6916541B2 (en) 2001-09-07 2005-07-12 Penn State Research Foundation Modified substrates for the attachment of biomolecules
KR100488281B1 (ko) 2001-09-15 2005-05-10 아람 바이오시스템 주식회사 열 대류를 이용한 염기서열 증폭 방법 및 장치
EP1451584B1 (en) 2001-11-09 2011-05-11 3dbiosurfaces Technologies, LLC High surface area substrates for microarrays and methods to make same
US20030170686A1 (en) 2001-12-07 2003-09-11 Rene Hoet Method and apparatus for washing magnetically responsive particles
US7772383B2 (en) 2002-01-25 2010-08-10 The Trustees Of Princeton University Chemical PCR: Compositions for enhancing polynucleotide amplification reactions
US20030211488A1 (en) 2002-05-07 2003-11-13 Northwestern University Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection
US20060160078A1 (en) 2002-07-12 2006-07-20 Cardy Donald L N Lateral flow assay device and method
DE03707568T1 (de) 2002-07-23 2005-11-10 Microban Products Co. Antimikrobielles melaminharz und daraus hergestellte produkte
US20040064435A1 (en) 2002-07-26 2004-04-01 Ahmad-Maher Moubayed Clinical assessment and diagnostic tool for use with peristaltic pump
US20040018577A1 (en) 2002-07-29 2004-01-29 Emerson Campbell John Lewis Multiple hybrid immunoassay
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
JP4632788B2 (ja) 2002-09-20 2011-02-16 ニュー・イングランド・バイオラブズ・インコーポレイティッド 核酸のヘリカーゼ依存性増幅
KR100463203B1 (ko) 2002-12-10 2004-12-23 삼성전자주식회사 활성 영역을 구비하는 반도체 소자
WO2004055159A2 (en) 2002-12-12 2004-07-01 Chiron Corporation Identification of oligonucleotides for the capture, detection and quantitation of west nile virus
US20050221281A1 (en) 2003-01-08 2005-10-06 Ho Winston Z Self-contained microfluidic biochip and apparatus
JP2004263708A (ja) 2003-01-14 2004-09-24 Kyowa Metal Work Co Ltd ツインクラッチ式変速機
CA2513880A1 (en) 2003-01-21 2004-08-05 Micronics Inc. Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing
US20040209309A1 (en) 2003-02-27 2004-10-21 Muldoon Mark Thomas Compositions and methods for the specific detection of mammalian muscle proteins
TWI333545B (en) 2003-04-02 2010-11-21 Cholestech Corp Adhered membranes retaining porosity and biological activity
FR2856046B1 (fr) 2003-06-16 2005-07-29 Biomerieux Sa Microvanne fluidique a ouverture par commande electrique
US7722817B2 (en) 2003-08-28 2010-05-25 Epocal Inc. Lateral flow diagnostic devices with instrument controlled fluidics
EP1670356A1 (en) 2003-09-03 2006-06-21 Life Patch International, Inc. Personal diagnostic devices and related methods
US7354706B2 (en) 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
WO2005026665A2 (en) 2003-09-15 2005-03-24 Diagnoswiss S.A. Microfluidic flow monitoring device
JP2005110621A (ja) 2003-10-10 2005-04-28 Aisin Seiki Co Ltd 核酸増幅方法及び核酸増幅用試薬キット
CN100540313C (zh) 2003-10-24 2009-09-16 索尼株式会社 头模块和液体喷射装置以及它们的制造方法
CN1930304B (zh) 2003-11-21 2016-01-06 Anp技术公司 不对称支化的聚合物缀合物和微阵列检测
JP4250523B2 (ja) 2003-12-25 2009-04-08 株式会社東芝 マイクロリアクタ、分析システム、分析方法、反応システム、反応方法、分離システム、分離方法
AU2005231107B8 (en) 2004-03-30 2011-04-14 Global Life Sciences Solutions Usa Llc Lateral flow format, materials and methods
US20050250141A1 (en) 2004-03-30 2005-11-10 Lambert James L Diagnostic assays including multiplexed lateral flow immunoassays with quantum dots
CA2562775C (en) 2004-04-07 2015-08-04 Access Bio, Inc. Nucleic acid detection system
US8173078B2 (en) 2004-04-28 2012-05-08 Industrial Technology Research Institute Gravity-driven micropump
US7273590B2 (en) 2004-04-29 2007-09-25 Industrial Technology Research Institute gravity-driven apparatus and method for control of microfluidic devices
US7796266B2 (en) 2004-04-30 2010-09-14 Kimberly-Clark Worldwide, Inc. Optical detection system using electromagnetic radiation to detect presence or quantity of analyte
CA2566962A1 (en) 2004-05-21 2005-12-01 Atonomics A/S Surface acoustic wave sensor comprising a hydrogel
SE527036C2 (sv) 2004-06-02 2005-12-13 Aamic Ab Analysanordning med reglerat flöde och motsvarande förfarande
CA2588820A1 (en) 2004-11-30 2006-06-08 Global Technologies (Nz) Ltd Method of sample analysis and apparatus therefor
US20060127886A1 (en) 2004-12-15 2006-06-15 Kaylor Rosann M Sample-efficient lateral flow immunoassay
JP2006185972A (ja) 2004-12-27 2006-07-13 Nikon Corp マスク及び露光方法
DK1843850T3 (en) 2005-01-31 2015-12-07 Realbio Technologies Ltd LATERAL FLOW STEERING CAPILLAR DEVICE
US7396689B2 (en) 2005-02-04 2008-07-08 Decision Biomarkers Incorporated Method of adjusting the working range of a multi-analyte assay
WO2006098804A2 (en) 2005-03-11 2006-09-21 Chembio Diagnostic Systems, Inc. Dual path immunoassay device
EP1871826A4 (en) 2005-03-31 2011-08-17 Fujifilm Corp SURFACE GRAFT MATERIAL AND MANUFACTURING METHOD THEREOF, ELECTRICALLY CONDUCTIVE MATERIAL, METHOD FOR MANUFACTURING THE SAME, AND MATERIAL WITH ELECTRICALLY CONDUCTIVE PATTERN
US7439079B2 (en) 2005-04-29 2008-10-21 Kimberly-Clark Worldwide, Inc. Assay devices having detection capabilities within the hook effect region
WO2006122312A2 (en) 2005-05-11 2006-11-16 The Trustees Of The University Of Pennsylvania Methods of testing using a microfluidic cassette
JP5033791B2 (ja) 2005-05-23 2012-09-26 ファディア・アクチボラグ 二段階側流分析法および装置
US20070015166A1 (en) 2005-07-14 2007-01-18 Nilsen Thor W Lateral flow methods and devices for detection of nucleic acid binding proteins
AU2006287548A1 (en) 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
CN101400993A (zh) * 2005-11-10 2009-04-01 阿普尔拉股份有限公司 包括多孔聚合物电极的微流体系统
US20090053773A1 (en) 2006-01-20 2009-02-26 Toppan Printing Co., Ltd. Reaction Container and Dna Amplification Reaction Method
US7537917B2 (en) 2006-03-31 2009-05-26 Collins Michael J Microwave assisted PCR amplification of DNA
AU2007265628B2 (en) 2006-06-23 2012-12-06 Revvity Health Sciences, Inc. Methods and devices for microfluidic point-of-care immunoassays
WO2008105814A2 (en) 2006-08-22 2008-09-04 Los Alamos National Security, Llc Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
JP2008148690A (ja) 2006-11-22 2008-07-03 Fujifilm Corp マイクロチップを用いた核酸増幅方法およびマイクロチップ、それを用いた核酸増幅システム
EP1972938B1 (de) 2007-03-19 2014-05-14 Ivoclar Vivadent Teststreifen für die Bestimmung des Kariesrisikos
US8105783B2 (en) * 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
EP2183379B1 (en) 2007-08-01 2015-04-15 Dana Farber Cancer Institute Enrichment of a target sequence
TW200909338A (en) 2007-08-23 2009-03-01 Ind Tech Res Inst Autonomous microfluidic apparatus
US20090086357A1 (en) 2007-09-28 2009-04-02 Toshiba America Information Systems, Inc. In drive written spirals for self servo writing
CN101409933B (zh) 2007-10-11 2010-08-04 华为技术有限公司 一种释放资源的方法和设备
US9409166B2 (en) 2007-12-10 2016-08-09 The Trustees Of The University Of Pennsylvania Integrated PCR reactor for cell lysis, nucleic acid isolation and purification, and nucleic acid amplication related applications
DE102007062441A1 (de) 2007-12-20 2009-06-25 Aj Innuscreen Gmbh Mobiles Schnelltestsystem für die Nukleinsäureanalytik
RU2533234C2 (ru) 2008-02-22 2014-11-20 Орион Диагностика Ой Способ и устройство для определения анализируемого вещества
US8187808B2 (en) * 2008-02-29 2012-05-29 Northwestern University Barriers for facilitating biological reactions
EP3067694A1 (en) 2008-05-05 2016-09-14 Los Alamos National Security, LLC Lateral flow-based nucleic acid sample preparation device, integrated with passive fluid flow control
US20110160090A1 (en) 2008-05-05 2011-06-30 Los Alamos National Laboratory Nanocrystal-Based Lateral Flow Microarrays and Low-Voltage Signal Detection Systems
US7910309B2 (en) * 2008-07-31 2011-03-22 Los Alamos National Security, Llc Multiplexed lateral flow microarray assay for detection of citrus pathogens Xylella fastidiosa and Xanthomonas axonopodis pv citri
EP2172260A1 (en) 2008-09-29 2010-04-07 Corning Incorporated Multiple flow path microfluidic devices
US9034605B2 (en) * 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR
US8084272B2 (en) 2009-03-25 2011-12-27 Abbott Point Of Care Inc. Amelioration of heterophile antibody immunosensor interference
FR2945097B1 (fr) 2009-04-30 2011-06-03 Commissariat Energie Atomique Vanne microfluidique a usage unique.
JP5577681B2 (ja) 2009-11-30 2014-08-27 住友電気工業株式会社 半導体装置
WO2011087813A2 (en) 2009-12-22 2011-07-21 University Of Washington Capillarity-based devices for performing chemical processes and associated systems and methods
ES2930133T3 (es) * 2010-03-09 2022-12-07 Ande Corp Biochip con almacenamiento de reactivo
EP2553473A4 (en) * 2010-03-30 2016-08-10 Advanced Liquid Logic Inc DROPLET OPERATION PLATFORM
US9731297B2 (en) 2011-01-06 2017-08-15 Meso Scale Technologies, Llc. Assay cartridges and methods of using the same
EP3564392B1 (en) * 2010-12-17 2021-11-24 Life Technologies Corporation Methods for nucleic acid amplification
US10519492B2 (en) 2011-04-20 2019-12-31 Mesa Biotech, Inc. Integrated device for nucleic acid detection and identification
BR112015010695B1 (pt) * 2013-01-11 2023-03-07 Becton, Dickinson And Company Dispositivo microfluídico e método para realizar um ensaio de uma amostra líquida, método para formar um dispositivo microfluídico, sistema e kit
US20160310948A1 (en) 2015-04-24 2016-10-27 Mesa Biotech, Inc. Fluidic Test Cassette
WO2018195493A1 (en) 2017-04-21 2018-10-25 Mesa Biotech, Inc. Fluidic test cassette

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683202B1 (https=) 1985-03-28 1990-11-27 Cetus Corp
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683195B1 (https=) 1986-01-30 1990-11-27 Cetus Corp
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
WO1994023055A1 (en) 1993-03-30 1994-10-13 United States Biochemical Corporation Cycle sequencing with non-thermostable dna polymerases
US6214587B1 (en) 1994-03-16 2001-04-10 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BURKE, SCIENCE, 1998
J. B. FINDLAY; S. M. ATWOOD; L. BERGMEYER; J. CHEMELLI; K. CHRISTY; T. CUMMINS; DONISH; T. EKEZE; J. FALVO; D. PATTERSON, CLIN. CHEM., vol. 39, no. 9, 1993, pages 1927 - 1933
LUO, NUC ACIDS RES., 2005
P.WILDING; M. A. SHOFFNER; L. J. KRICKA, CLIN.. CHEM., vol. 40, 1994, pages 1815 - 1817
SAMBROOK; RICH; MANIATIS: "Molecular Cloning and Laboratory Manual", 2003, COLD SPRING HARBOR
See also references of EP2699698A4
WILDING, NUC. ACIDS. RES., 1996

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10458978B2 (en) 2006-08-22 2019-10-29 Triad National Security, Llc Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US10337056B2 (en) 2007-03-28 2019-07-02 Fluoresentric, Inc. Dynamic flux nucleic acid sequence amplification
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
US11293058B2 (en) 2011-04-20 2022-04-05 Mesa Biotech, Inc. Oscillating amplification reaction for nucleic acids
US11999997B2 (en) 2011-04-20 2024-06-04 Mesa Biotech, Inc. Integrated device for nucleic acid detection and identification
US12421543B2 (en) 2011-04-20 2025-09-23 Mesa Biotech LLC Oscillating amplification reaction for nucleic acids
US11268142B2 (en) 2011-04-20 2022-03-08 Mesa Biotech, Inc. Integrated device for nucleic acid detection and identification
US10519492B2 (en) 2011-04-20 2019-12-31 Mesa Biotech, Inc. Integrated device for nucleic acid detection and identification
US10370707B2 (en) 2013-10-09 2019-08-06 Fluoresentric, Inc. Multiplex probes
EP3055430A4 (en) * 2013-10-09 2017-06-21 Fluoresentric, Inc. Multiplex probes
US12023672B2 (en) 2015-04-24 2024-07-02 Mesa Biotech, Inc. Fluidic test cassette
US12128414B2 (en) 2015-04-24 2024-10-29 Mesa Biotech LLC Automated method for performing an assay
US11572580B2 (en) 2017-06-07 2023-02-07 Takara Bio Inc. Oligonucleotide preservation method
WO2020035698A1 (en) * 2018-08-17 2020-02-20 Touchlight IP Limited Synthesis of dna with improved yield
EP4437124A4 (en) * 2021-11-24 2025-10-08 D Nome Private Ltd RAPID METHOD FOR ISOTHERMAL AMPLIFICATION MEDIATED BY REVERSE TRANSCRIPTION LOOP-LAMPS AT ROOM TEMPERATURE (RT-LAMP) AND REAGENT KIT
WO2023159127A1 (en) * 2022-02-17 2023-08-24 One Lambda, Inc. Systems and methods for protein detection
WO2024083473A1 (de) * 2022-10-19 2024-04-25 Robert Bosch Gmbh Verfahren und vorrichtung zur voramplifikation eines nukleinsäuregemisches

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PT2699698T (pt) 2017-04-11
CA2856304A1 (en) 2012-10-26
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KR20140044323A (ko) 2014-04-14
ES2583135T3 (es) 2016-09-19
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EP2699698A2 (en) 2014-02-26
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