WO2001023938A1 - Systeme d'observation d'image au microscope, procede de controle, et support enregistre lisible par l'ordinateur a programme de controle enregistre - Google Patents

Systeme d'observation d'image au microscope, procede de controle, et support enregistre lisible par l'ordinateur a programme de controle enregistre Download PDF

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Publication number
WO2001023938A1
WO2001023938A1 PCT/JP2000/006739 JP0006739W WO0123938A1 WO 2001023938 A1 WO2001023938 A1 WO 2001023938A1 JP 0006739 W JP0006739 W JP 0006739W WO 0123938 A1 WO0123938 A1 WO 0123938A1
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WO
WIPO (PCT)
Prior art keywords
image
microscope
sample
observation system
information
Prior art date
Application number
PCT/JP2000/006739
Other languages
English (en)
Japanese (ja)
Inventor
Akitsugu Kagayama
Shuji Nakagawa
Original Assignee
Olympus Optical Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Optical Co., Ltd. filed Critical Olympus Optical Co., Ltd.
Priority to KR1020017006096A priority Critical patent/KR20010092741A/ko
Publication of WO2001023938A1 publication Critical patent/WO2001023938A1/fr

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes

Definitions

  • Microscope image observation system its control method, and recording medium that can be read by a computer that records the control program
  • the present invention relates to, for example, a microscope image observation system used for observing a pathological specimen, a control method thereof, and a recording medium which can be read by a computer that records the control program.
  • a pathologist sets a rough observation policy by visually observing the entire preparation in which the sample is set, and then performs microscopic observation at low magnification to perform initial diagnosis. After that, diagnostic procedures such as performing detailed observations at high magnification were performed.
  • the client takes an image of the entire specimen on the preparation with a TV camera in a macro imaging stand. Then, the captured macro image is loaded into the frame memory connected to the information processing device (requesting terminal). The frame memory digitizes the captured image and stores it in a monitor connected to the information processing device or a TV monitor connected to the frame memory. Display as a mouth image.
  • the above is the initial diagnosis process.
  • the prepared plate is mounted on the motorized X-Y stage of the optical microscope, and the specimen is photographed with the TV camera of the optical microscope. After this imaging, the captured images are captured and digitized in the frame memory as in the initial diagnosis. Then, it is displayed as a microscope image on a monitor connected to the information processing device or on a TV monitor connected to the frame memory. Thereby, detailed observation at high magnification is performed.
  • images captured in a frame memory connected to such an information processing device include a pathologist located at a remote location via a public line such as an ISDN. It is transferred to the information processing device of the observation terminal on the hospital side (hereinafter referred to as the observation side). Then, the image is displayed on the monitor of the information processing device. This allows the client to make a pathological diagnosis by a remote pathologist (observer).
  • a mesh is set at the position to be observed on the macro image obtained in the initial observation, and the area of each grid set by the mesh is automatically automatically imaged. You can also import.
  • the entire specimen image on the slide is photographed by a TV camera in a macro image stand.
  • the obtained macro image is captured by the frame memory connected to the information processing device, the image is digitized, and the monitor or frame memory connected to the information processing device is digitized. It can be displayed on a TV monitor connected to a remote control.
  • the main body of the microscope and the macro image shooting stand are separate parts. For this reason, it was necessary for the client to transfer the prepared slide from the mouth image stand to the motorized X-Y stage of the optical microscope.
  • This work involves placing the prepared plate on a macro image capturing stand and capturing the positional relationship between the captured images and placing the prepared sample on the motorized X-Y stage of the optical microscope. It is difficult to exactly match the positional relationship when fixed.
  • the remote information processing device on the observation side controls the information processing device on the requesting side to perform observation and remote control
  • the macro operation is performed using the macro photography stand.
  • To take an image and place it on the motorized X-Y stage of the optical microscope it is necessary to manually instruct the worker on the requesting side from the observer to replace it.
  • work efficiency was deteriorated because the work could not be performed automatically.
  • the present invention provides a microscope image observation system capable of recognizing the entire image of a specimen, and at the same time, easily grasping the actual observation position and range on the specimen. It is an object of the present invention to provide a recording medium that can be read by a computer that records a control method and a control program.
  • an apparatus for controlling a microscope by using a programmed computer comprising: an image acquiring unit configured to acquire an image of a specimen at a predetermined magnification; and Sample placement means for positioning and placing the sample; movement means for relatively moving the sample and the image acquisition means; position detection means for detecting the position of the sample; and acquiring an image of the sample.
  • Magnification storage means for storing a magnification of the specimen, a position recognition image for recognizing a position on the specimen mounting means, and a position of the specimen detected by the position detection means and the magnification storage means.
  • a microscope image observation system is provided.
  • the indicator is an image of a specimen included in an acquisition area of the image of the specimen acquired by the image acquisition means.
  • the operator can easily grasp not only the position where the operator is actually observing, but also the image being observed.
  • the calculating means is adapted to display the index so that the relative position between the position recognition image and the index changes in proportion to and in conjunction with the amount of movement by the moving means. Find the display position.
  • the operator's instruction to move the observation position can be grasped on the position recognition image in real time. Therefore, the desired position of the sample can be ascertained, and the operator can confirm whether or not a range to be grasped in detail later is included, and the range to be observed in detail can be easily set.
  • the display unit displays a position and a range of an image newly obtained by the image obtaining unit, and the display unit displays the combined image on the position recognition image.
  • input means for inputting the position and range of the shoe As a result, a mesh is displayed on the position recognition image, and the range of the sample to be surrounded by the mesh can be easily specified.
  • an apparatus for controlling a microscope using a programmed computer comprising: an image acquisition unit configured to acquire an image of a specimen at a predetermined magnification; and Sample placement means for positioning and placing the sample; moving means for relatively moving the sample and the image acquisition means; position detection means for detecting the position of the sample; magnification for acquiring the image of the sample
  • a magnification storage means for storing the magnification information obtained by the image acquisition means, position information detected by the position detection means, and magnification information obtained by the acquisition magnification change means.
  • a line connection means capable of transmitting information to an external terminal.
  • a microscope image observation system for controlling a microscope by using a programmed computer, comprising: a line connecting means capable of communicating information; From a microscope image observation mechanism provided with a sample mounting means for arranging and mounting, an image obtaining means for obtaining an image observed by a microscope at a predetermined magnification, and a position detecting means for detecting a position of a sample of the microscope Acquiring means for acquiring image information, magnification information, and position information via the line connection means; andgenerating a position recognition image for recognizing a position on the sample mounting means.
  • a microscope image observation system is provided, wherein a second display means for displaying an image obtained by synthesizing the index is provided.
  • the input means for selecting and moving an index on the position recognition image displayed on the display means, and the moving amount of the moving means corresponding to the selection and moving operation of the index by the input means are calculated.
  • a moving amount calculating means wherein the moving means moves according to the moving amount obtained by the moving amount calculating means.
  • the index is an image having a predetermined area
  • the microscope image observation system further includes a reference position display means for displaying a reference position on the index, and a reference position display means.
  • Calculating means for calculating the correspondence between the position on the sample mounting means and the position on the position recognition image by adjusting the reference position of the sample to the displayed reference position. Accordingly, even when the present invention is applied to a microscope system having no means for fixing a specimen such as a clean-meal, for example, the relationship between the actual specimen position and the positional relationship on the position recognition image can be obtained. An association is made.
  • the present invention relating to the device is also established as an invention relating to a method
  • the present invention relating to a method is also established as an invention relating to an apparatus.
  • the present invention relating to an apparatus or a method is provided for causing a computer to execute a procedure corresponding to the present invention (or for causing a computer to function as a means corresponding to the present invention, or a computer).
  • the present invention is also established as a recording medium readable by a computer that records a control program (for realizing a function equivalent to the invention on a computer).
  • FIG. 1 is a diagram showing an entire configuration of a microscope image observation system according to a first embodiment of the present invention.
  • Figure 2 shows the client terminal and the microscope Configuration diagram.
  • FIG. 3 is a view showing a flowchart of a microscope image observation method according to the embodiment.
  • FIG. 4 is a view showing a display example of a preparation simulation window according to the embodiment.
  • FIG. 5 is a view for explaining the correspondence between the movement of the electric stage and the movement of the image of the preparation according to the embodiment.
  • FIG. 6 is a diagram showing a specific configuration example of a mesh according to the embodiment.
  • FIG. 7 is a diagram showing a relationship between a mesh outer frame and movement of a reduced image according to the embodiment.
  • FIG. 8 is a diagram showing a method for setting a mesh outer frame according to the embodiment.
  • FIG. 9 is a view showing an example of a screen displayed on a monitor for instructing movement of the electric stage according to the embodiment.
  • FIG. 10 is a diagram showing a flow chart of a microscope image observation method according to a second embodiment of the present invention.
  • FIG. 11 is a view showing a display example of a window ⁇ without using an original image according to the embodiment.
  • FIG. 12 is a view showing a display example of a preparation simulation window 2 according to the embodiment.
  • FIG. 13 is an enlarged view of a reduced image according to the embodiment.
  • FIG. 1 shows a schematic configuration of a microscope image observation system of the present invention.
  • 100 is a requesting terminal
  • 200 is a requested side, that is, an observation side terminal.
  • the requesting terminal 100 has a computer 1
  • the observation terminal 200 has a computer 8.
  • These computers 1 and 8 are connected to the communication units via line connection devices 2 and 9 and a communication line (for example, ISDN) 20.
  • the computer 1 of the requesting terminal 100 has a monitor 3 having a display function for displaying a sample image, and also has a software of the computer 1.
  • a keyboard 5 and a mouse 4 are provided as means for operating and inputting data.
  • the computer 1 of the requesting terminal 100 is further connected to the microscope 14 via the interface 19.
  • the microscope 14 is a motorized stage 15 in which a slide (not shown) is fixed with a clamp, and an objective lens for capturing the specimen image on the platen of the motorized stage 15.
  • the motorized revolver 17 that automatically exchanges the objective lens 16 for one with a different magnification and the specimen image captured via the objective lens 16 are captured, that is, the image acquired by the microscope (microscope) It has a video camera 18 for acquiring images.
  • the microscope 14 is provided with an auto-focus unit, a dimming function, a motorized aperture mechanism, and the like (not shown) in addition to the above.
  • the computer 1 of the requesting terminal 100 has a video capture function, and receives the image output of the video camera 18 that captures a microscope image.
  • Various terminals including terminals Have a child.
  • the computer 1 has a storage unit for storing image information output from the video camera 18 etc., but the computer 1 has additional peripheral devices. It goes without saying that a recording device such as an MO (magneto-optical disk) may be attached.
  • MO magnetic-optical disk
  • a hand-free talker 7 is connected to the line connection device 2. According to the hand-free talker 7, it is possible to realize a conversation between the requesting side and the observing side while operating the personal computer without holding the handset.
  • the video power camera 18 mounted on the microscope 14 is connected to the personal computer 1, but it is connected via a video selector or the like (not shown). An imaging device can be further connected.
  • the personal computer 8 of the observation side terminal 200 also has a monitor 10 having a display function for displaying a sample image, and also includes a monitor.
  • a keyboard 12 and a mouse 11 are provided as means for operating the software of the computer and for inputting data.
  • inside the computer 8 there is a storage unit for storing the image information transferred from the computer 1 of the requesting terminal 100.
  • a recording device such as an MO (Magneto-Optical Disk) may be attached as a peripheral device of the computer 8.
  • a hand-free telephone 13 can be connected to the line connection device 9.
  • FIG. 2 is a block diagram showing a specific configuration of the requesting terminal 100. As shown in FIG. The requesting terminal 100 communicates with the microscope 14 and various control signals to the microscope 14 as described above, and the video camera 18 attached to the microscope 14 These images are taken from the personal computer 1 that performs various processes described below.
  • the microscope 14 mounts a video camera 18 for capturing a microscope image, an electric revolver 17 for switching the objective lens 16 as an acquisition magnification changing means, and a not-shown preperator.
  • the motorized stage 15 can be moved in a plane orthogonal to the optical axis of the microscope 14 while being fixed with the crane, and the motorized stage 15 can be used as position detecting means. It has an encoder 21 that detects the moving position of the camera.
  • the motorized stage 15 is provided with an origin sensor 31 for detecting the origin position of the stage as a reference when the stage is moved. For example, when the system is started, the electric stage 15 moves and the origin sensor 31 detects the origin position to find the origin, and the electric stage 15 moves and moves based on the origin. The position is detected by encoder 21.
  • the personal computer 1 has a CPU 29 that controls each device and program, and a CPU node 28 that connects the CPU 29 to a control circuit.
  • a recording medium 27 for recording a computer program is connected to 28.
  • the recording medium 27 has a control program 27 a for executing operations of the microscope 14 and control of a GUI (graphics user interface) described below. are doing.
  • This recording medium 27 includes a flexible disk, a node disk, a CD-ROM, and a memory. Any format can be used as long as programs such as cards can be recorded.
  • such a control program is recorded on the server computer via a network connected to the server computer (not shown). It is also possible to download a control program to a personal computer and execute it.
  • the CPU No. 28 has a data storage device 26 for storing information such as data and programs necessary for processing by the CPU, and a video camera 1 for photographing a microscope image as an image acquiring means. Input video signals from 8 and digitize the image.Video capture 22 and video card 23 that can display the image and GUI digitized by video capture 22 are connected. Has been done. This video card 23 actually displays images and GUIs. Monitor 3 outside of computer 1 is connected.
  • the CPU node 28 has a mouse 4 and an input means from an operator outside the computer 1 via an input / output interface 24. It is connected to the keyboard 5, and furthermore, the motorized revolver 17 of the microscope 14, the motorized stage 15 and the encoder 2 are connected via the input / output interface 25. 1 is connected to enable control from the computer.
  • the CPU bus 28 is connected to a line connection device 2 external to the computer 1 via an input / output interface 30 and further to a public line ( It is connected to a remote observation terminal 200 (not shown) via ISDN) 20.
  • FIG. 2 the configuration of the requesting terminal 100 has been described.
  • the computer 8 of the observation terminal 200 is also the same as the computer 1 of the requesting terminal 100 except that the microscope 14 and the video capture 22 are not included, and therefore, the description is omitted. .
  • the motorized stage 15 should have its stage originated before the observation.
  • the origin of the stage obtained here is used as a reference position for calculating the movement amount and movement position when the electric stage 15 is moved in the later control.
  • the origin position can be detected by using the position detected by the origin sensor 31 in Fig. 2 as the origin position, or by using a pulse motor (not shown) that drives the motorized stage 15 from the origin sensor 31.
  • a pulse motor (not shown) that drives the motorized stage 15 from the origin sensor 31.
  • a specimen for example, a preparation on which cells are placed is fixed on the motorized stage 15 of the microscope 14.
  • the electric stage 15 has Kremmel a and b as shown in Fig. 5 as 51 and 52 as fixing jigs for preparats.
  • These clean a51 and clean b52 are arranged at predetermined positions on the stage, and are physically connected to the stage.
  • the target position is predetermined.
  • the preparation is fixedly arranged at a predetermined position by Clenmel a51 and Clenmel b52. Therefore, the prenomination by Klemmmenore a51 and Klemmenole b52. Preno, by fixing the lamp. The positioning of the lamp is performed.
  • the origin of the stage is determined in advance, such a fixation also determines the positional relationship between the origin of the electric stage 15 and the prepara- tor.
  • the clean-melt is used, but the fixing method does not matter as long as the positional relationship between the origin and the preparation can be determined.
  • a simulated image including a preparator which is the position recognition image 47, is displayed on the monitor 3.
  • Window (hereinafter referred to as a preparation simulation window) 40 is displayed (step S10).
  • FIG. 4 shows a display example of a preparation simulation window 40, which is displayed on the monitor 3.
  • a window is a screen for displaying and inputting information using Microsoft's operating software and window technology.
  • step S11 "is the input of the change of the objective lens?", It is confirmed whether there is an instruction to change the objective lens. If the change of the objective lens is input (Y), the Proceed to step S12 to change to the specified objective lens. Specifically, the electric revolver 17 is rotated to select the designated objective lens 16. If the change of the objective lens 16 is not entered (N), the next step is performed using the already set objective lens 16 without passing through the step S12. Proceed to.
  • step S13 it is confirmed whether or not a stage movement instruction has been issued in "Is a stage movement instruction input?" If the instruction to move the stage is received (Y), the process proceeds to step S14, and the electric stage 15 is moved to the designated position. If there is no stage move instruction input (N), go to the next step at the position already set.
  • a button 50 as shown in FIG. 9 is displayed on the window, and the operator uses the mouse 4 or keyboard 5 input means. It can be moved by clicking the button.
  • the button 50 in FIG. 9 is clicked, the electric stage 15 is driven to a not-shown motor so that the electric stage 15 moves in the same direction as the arrow of each button. A pulse is sent and movement is controlled.
  • a microscope image from the video camera 18 is taken into the personal computer 1 as a digital image. Then, the image captured in “Preno. Displaying the original image in the simulation screen of the update” in step S16 is displayed.
  • the prepared simulation window 40 there is a rearnote time (the screen is drawn at any time in video rate or near speed).
  • the original image 41 is displayed as an image or a still image. This image was taken with a video camera 18 of a microscope 14. It is displayed by being synthesized with a window on a video card 23 via a video capture 22 in a computer 1.
  • the original image 41 is displayed alongside the original image 43, the original image 43, the original image 44, and the original image.
  • An image 47 for position recognition consisting of the lights 45 is displayed.
  • This position recognition image 47 is an image for recognizing the position of each component on the motorized stage 15, and is a preparator on the motorized stage 15 of the microscope 14. It is desirable that the configuration diagram be displayed so that the vertical and horizontal ratios are the same scale ratio as in the configuration of the real object fixing 53. Of course, it is not necessary to match the scale of the actual composition completely.
  • step S17 a process of "converting the captured image to the scale of the preparation on the preparation simulation window" is performed.
  • the number of pixels of the captured image is converted according to the scale of the simulation model window 40 to create a reduced image 42.
  • the display direction of the reduced image 42 may be inverted up, down, left, and right depending on the configuration of the optical system of the microscope.
  • the image may be displayed upside down, left and right.
  • step S 18 proceed to step S 18, where “The stage position is detected.
  • the position of the motorized stage 15 is detected by the encoder 21, and the position of the motorized stage 15 is detected by the encoder 21.
  • the XY coordinates are obtained based on the obtained origin, and the position of the stage is determined by the drive pulse to the noise motor that drives the motorized stage 15 without using the encoder 21.
  • Ru can and call Ru demand even as a child to mosquito window down bet the number of pulses.
  • a known whether the drive Panoresu 1 Roh / Les Nono 0 Noresu electric stage 1 5 come to have input to the motor to move what mm Therefore, if the number of pulses from the origin is obtained, the position of the stage can be obtained by the force S.
  • the motorized stage 15 moves 0.01 mm with one pulse. If designed, the motorized stage 15 will move 1 mm with 100 pulses. Become.
  • the number of pulses when the motorized stage 15 moves in step S14 is counted. This number of pulses is stored in the data storage device 26. Then, it is preferable that the number of pulses is read out and converted into the movement amount of the electric stage 15.
  • step S 19 processing of “display a reduced image at a position corresponding to the position where the real image is observed with the blur parameter” is performed.
  • the position force of the electric stage 15 obtained in step S18 is used to move the position of the electric motor simulation window 40 to the position on the lever 45. Convert.
  • the image obtained in step S17 is displayed as the reduced image 42 at the converted position.
  • the reduced image 42 is displayed as an index for associating the position recognition image 47 with the position on the electric stage 15. That is, by observing the reduced image 42, the operator can visually grasp the relative positional relationship between the position recognition image 47 and the motorized stage 15. Can be done. More specifically, it is possible to visually grasp the relative positional relationship between the position recognition image 47 and the image acquisition position.
  • the apparatus coordinate system of the motorized stage 15 and the preparer It is necessary to match the reference of the graphic display coordinate system for displaying the image of the simulation window 40 or clarify the positional relationship.
  • FIG. 5 (a) simply shows the motorized stage 15 and the objective lens 16.
  • the Z axis is the optical axis (center axis) of the objective lens 16 that is perpendicular to the movement plane of the motorized stage 15 and serves as the reference for stage movement.
  • the X axis and the Y axis are perpendicular to the Z axis. Determine the axis coordinate system.
  • the stage is parallel to the XY plane, and the intersection of the X axis and the Y axis is the origin O (here, the origin O is on the stage plane).
  • the origin O may be assumed to be the stage origin determined by the origin search, or may be assumed to be a position offset from the stage origin determined by the origin search. Origin from above The distance from the stage origin obtained in the output is a known value.
  • the coordinate system of the system is determined based on the origin O. Click Les Nme Le a 5 1 cis-te-time coordinate system by Ri origin O mosquito et al view of an individual 5 (b), click-les-down menu Norre b 5 2, the position of the flop Leno ⁇ 0 La over door 5 3 and the known Become .
  • Clenmel a 51 and Clenmel b 52 are mechanically fixed to the electric stage 15, and the slide 53 is removable.
  • the size of the preparation 53 is already known, and since it is placed against the above-mentioned clamp, the coordinate position of the preparation 53 is also finally determined. It becomes known.
  • the position recognition image 47 is displayed as shown in FIG. 5 (c). Display in the graphic of the computer. That is, based on the position information, click as with real vertically and horizontally the same scale les down menu Le a 4 3, click Les down menu Le b 4 4, flop Leno ⁇ 0 La over preparative 4 5 portrayal Mr.
  • the origin O 'of the position recognition image 47 with respect to the origin O of the system coordinate system is determined and used as a reference when the reduced image 42 is displayed.
  • Clenmel a51 and Clenmel b52 are for the real space on the electric stage 15 and Clenmels 43 and 44 are for position recognition. This figure shows Clen Menole on Image 47.
  • the scale values of the system coordinate system and the position recognition image 47 are known, if the motorized stage 15 moves a certain distance in the system coordinate system, it corresponds to that distance.
  • the moving distance on the position recognition image 47 can be calculated from the scale ratio.
  • the reference point of the system coordinate system and the reference simulation window are the same. Since they match and the scale ratio is known, the positional relationship between the position of the electric stage 15 and the position recognition image 47 of the preparation simulation window 40 is established.
  • the observation point shown in Fig. 5 (b) is from the position of the origin O on the slide 53, and as shown in Fig. 5 (d), the electric stage 15 is moved in one direction of the X-axis (left ), The observation position is point P on Fig. 5 (d).
  • the length of the line O P between the origin O and the point P is the moving distance.
  • This moving distance can be detected by the encoder 21 shown in FIG. Also, it is not necessary to use encoder 21. Luth motor drive noise is 1 ⁇ . Since it is known how many millimeters the electric stage 15 can be moved per lus, the distance can be obtained from the total number of driving pulses spent for movement.
  • a reduced image moving position of the position recognition image 47 on the preparation simulation window 40 is obtained. If the position observed in FIG. 5 (b) is the origin O, the center position of the reduced image 42 displayed in FIG. 5 (c) is ⁇ ,.
  • the motorized stage 15 is moved from the position shown in FIG. 5 (b) by a line OP in one direction of the X-axis, as shown in FIG. Move to the position where The moving direction of the reduced image 42 is opposite to the moving direction of the stage, and the calculation of the display position is based on the moving distance of the line OP. It can be calculated from the scale ratio of the standard system and the preparation simulation window 40.
  • the reduced image 42 on the slide simulation window 40 moves in conjunction with the movement of the motorized stage 15 to move the slide to the operator. This makes it easy to see which part of the object you are observing.
  • the reduced surface image 42 shows the sample that is actually being observed, it is possible to visually determine which position of the sample is located on which position on the specimen. It is easy to find and the desired observation position can be easily found.
  • it is possible to prevent erroneous operations such as moving the motorized stage 15 too much and moving the observation position out of the preparation 53.
  • the reduced image 40 is displayed by reducing the original image 41 in accordance with the scale ratio.
  • the frame is not limited to an image but indicates a range. It may be an index such as a + character indicating the position or position.
  • the X 'axis, the Y' axis and the origin O 'in FIG. 5 (c) may be shown as indices.
  • step S20 After displaying the reduced image 42 as an index of the observation position in step S19 in this way, the process proceeds to step S20.
  • step S 20 a mesh outer frame as shown in FIG. 6 is displayed on the planar 45 of the position recognition image 47 in FIG. In this case, the mesh outer frame 46 of the mesh capturing range is displayed on the preparation 45.
  • a grid of length m x width n (3 x 3 mesh 62 in Fig. 6) is set for the sample to be observed, and the object is sequentially located at positions corresponding to the grid.
  • the image is acquired with the lens magnification increased (in Fig. 6, a total of 9 images can be acquired).
  • the number of grids can be one IX or one frame.
  • step S21 proceed to "Is an instruction to change the mesh position and size input?", And an instruction to change the position of the mesh outer frame 46 and the size is issued. Check if it is. If the change of the position and the size of the mesh outer frame 46 is input (Y), the step S22 "Preno of the position recognition image 47. Change and specify the mesh size position of "" and change the specified size and position of the mesh outer frame 46. If the mesh position / size change instruction is not input (N), the process proceeds to the next step using the already set mesh position / size.
  • the mesh outer frame 46 on the plano 45 in step S21 is, for example, as shown in FIG. 7 (a), when the operator is using the mouse 4 or the keyboard. Drag and move the four corners of the frame using the input means of 5. As a result, the size of the mesh outer frame 46 can be changed. Also, by dragging and moving the mesh outer frame 46, the entire mesh outer frame 46 can be moved up, down, left and right. In the display of the mesh outer frame 46, not only the outer frame of the mesh but also the range to be actually divided can be displayed in a lattice pattern by fitting the outer frame to the outer frame. Note that the mouse pointer 60 is displayed on the original image 41 as shown in FIG.
  • the mouse pointer 60 is displayed on the original image 41 to select a desired mesh range.
  • the position of the corner of the mesh outer frame 46 (upper right in the case of FIG. 7B) can be set.
  • the mesh outer frame 46 is set, it is overlaid on the original image 41.
  • the button 61 is displayed on the monitor 3 as shown in FIG. 7 (c), and the operator clicks the button using the mouse 4 or the keyboard 5 input means.
  • the four corners of the target may be selected, and the range of the mesh outer frame 46 of the desired specimen may be set based on the selected four corners.
  • the relationship between the mesh outer frame 46 and the movement of the reduced image 42 will be described with reference to FIG.
  • the reduced image 42 is moved on the prepatter 45 of the position recognition image 47 in conjunction with the movement of the stage 15. Moving.
  • the reduced image 42 is moved to the position shown in FIG. 8A by force S, if the hatched portion in the reduced image 42 is a portion desired to be observed, such as a cell, the mesh outer frame 4 6 It is possible to visually predict (recognize) the presence of cells inside.
  • the upper right corner of FIG. 8 (b), the lower right corner of FIG. 8 (c), and the lower left corner of FIG. Power at the upper right corner of FIG. 8 (b), the lower right corner of FIG. 8 (c), and the lower left corner of FIG. Power.
  • the mesh outer frame 46 is set narrower than in FIGS. 8 (a) to 8 (d).
  • the reduced image 42 overlaps the mesh outer frame 46. And overlap with the cells desired to be observed. Similarly, the figure
  • the cell position also overlaps the mesh outer frame 46. In this state, if the images are sequentially captured in the mesh, the entire portion (cell) desired to be observed cannot be completely captured.
  • the motorized stage 15 may be moved.
  • the mesh outer frame 46 on the original image 41 is also moved in conjunction with the movement of.
  • step S23 "Measurement instructing force by mesh S force?", And check whether the meshing force is instructed. . If there is a mesh input (Y), the flow advances to step S24 to start the sequential acquisition of the range by the mesh and select "Specify in the mesh.” Change the magnification to the specified magnification ". In the present embodiment, the magnification of the objective lens 16 is changed by the motorized revolver 17, and the mesh size of the mesh is changed.
  • step S25 the process of "moving the motorized stage to the position for taking in the specimen specified by the mesh” is performed.
  • step S25 the observation position is moved to the position designated by the mesh on the electric stage 15.
  • step S26 the process proceeds to “image capture and storage” in step S26 to capture the image acquired by the microscope, and the image acquired by the microscope of the acquired specimen as a still image in the data storage device 2.
  • step S27 "mesh capture end force?", It was confirmed whether image capture and storage were completed for all meshes, and all necessary images were captured. If this is the case, the process of importing the mesh ends.
  • step S28 the process proceeds to “Is an end instruction input?” In step S28, and confirms whether a series of processes is to be ended. If there is a termination instruction, the processing is terminated. If there is no instruction, the above series of processing is repeated.
  • the prepared simulation window 40 is displayed on the monitor 3 and the prepared simulation window 40 is displayed.
  • the original image 41 is displayed at the same time, along with the original image 41, and the position consisting of the images of Clenmel a43, Clenmel b44, and Preparat 45 Display recognition image 4 7.
  • a reduced image 42 that is obtained by thinning out the pixels of the original image 41 is displayed on the preparator 45 in the position recognition image 47.
  • the preparation in the position recognition image in which a part of the sample corresponding to the original image 41 is displayed.
  • the operator can easily grasp the position, range, etc., actually observed on the lamp.
  • the position recognition image 47 since the position recognition image 47 only needs to know the position of the reduced image, it is only necessary to display a scale grid or a mesh outer frame instead of a preparation. good.
  • the specimen is desired.
  • the operator can check whether the desired range of the sample is in the mesh outer frame 46 or not, and the mesh can be easily set.
  • a mesh outer frame 46 is displayed on the original image 41 so that the four corners of the mesh can be specified.
  • the mesh itself can be moved to enable the mesh to be moved.
  • the range of the sample to be acquired can be easily specified with.
  • the request-side terminal 100 has been described.
  • operation from the remote-side observation terminal 200 is also possible.
  • FIG. 10 illustrates an operation sequence when operating from the observation-side terminal 200. As shown in Fig. 10, the processing of the requesting terminal 100 and the processing of the remote observation terminal 200 are remote and independent, but both processes start first. To make it operable.
  • Step S30 an initialization process is performed on the requesting terminal 100.
  • the image of the specimen is taken in a state where it can be captured, and the position of the motorized stage 15 and the magnification of the objective lens 16 currently used are stored in the data storage device 26.
  • a line connection process (step S31) with the observation side terminal 200 is performed.
  • a request for line connection is made from the requesting terminal 100 to the observing terminal 200 via the ISDN line 20 so that the cooperative processing of the terminals can be performed.
  • the line connection (step S45) is performed in response to the line connection request, and mutual information can be exchanged. Remote control is possible for 0.
  • the observation side terminal 200 performs a process of receiving initial data (step S46).
  • a request for transmitting initial data is transmitted from the observation terminal 200 to the requesting terminal 100, and the requesting terminal 100 receives the initial data as a response.
  • the requesting terminal 1000 transmits necessary initial data, such as the position of the objective lens and the motorized stage, to the observing terminal 200. (Step 32).
  • the observer terminal 200 displays a simulated preparatory window 40 on the window in the same manner as in step S10 in FIG. 3 ( Step S47).
  • the preparatory simulation window may be the same as that shown in FIG. 4, but the position recognition image 4 from which the original image shown in FIG. 11 (the original image 4 1 in FIG. 4) is omitted. Only 7 is acceptable.
  • the observation side terminal 200 proceeds to step S48, "Is the change of the objective lens input?", And confirms whether the instruction to change the objective lens 16 has been issued. .
  • the processing of the "change request to the specified objective lens" in step S49 is performed.
  • the observation side terminal 200 sends the change information of the objective lens 16 (for example, information such as change from 100 times to 400 times) to the requesting terminal 100. Send to 0 and request change of objective lens 16.
  • the requesting terminal 100 rotates the motorized revolver 17 of the microscope 14 in the process of “change to the designated objective lens” in step 33. Select the specified objective lens 16 (if not required, use the initially selected objective lens). Further, if there is no input of the change of the object lens 16 at the observation side terminal 200 (N), the process proceeds to the next step.
  • the observation-side terminal 200 displays the motorized stage 1 in step S50, "Is the stage movement instruction input?" Check if the move instruction of 5 has been given.
  • the process proceeds to “Request the stage to be moved to the designated position” in step S51, and the stage movement request is made. Perform processing.
  • the observation side terminal 200 transmits the designated position information to the requesting side terminal 100, and requests the electric stage 15 to move.
  • the requesting terminal 100 performs the process of “moving the stage to the designated position” in step S34.
  • the motorized stage 15 of the microscope 14 is moved to the designated position (if there is no request, the stage is not moved).
  • the process proceeds to the next step at the already set position.
  • the movement instruction of the electric stage 15 is made by pressing a button 50 as shown in FIG. It is displayed on the window of the computer 8, and the operator moves the mouse 4 or the keyboard 5 using the input means and clicking the button. Then, when the button 50 in FIG. 9 is clicked, the electric stage 15 is moved in the same direction as the arrow of each button so that the personal computer 100 of the requesting terminal 100 moves.
  • Conveyor 1 controls the movement by sending the drive panorama to the nose motor. (Because the observation terminal 200 is in a remote location, it is naturally possible to move the motorized stage 15 directly by hand. Absent) .
  • the observation side terminal 200 performs the original image data reception processing (step S52).
  • the transmission of the original image data is requested from the observing terminal 200 to the requesting terminal 100, and if there is a request, the requesting terminal 1000 proceeds to step S35. Proceed to “Transmission of original image data”, and take the microscope image from the video camera 18 into the computer 1 as a digital image and send it to the observation terminal 200.
  • the original image data is received in step S52.
  • step S47 if the position recognition plane image 47 shown in FIG. 11 is used as shown in step S47, the original image data is required. In addition, only the data of the reduced image is sufficient.
  • the requesting terminal 100 converts the original image into a reduced image, and sends the reduced image data to the observer.
  • the original image has a large amount of data, and if it is transmitted over the communication line (ISDN 20), the communication line will be congested, and the speed of processing at each terminal will be delayed. Cause obstacle.
  • the reduced image has a smaller amount of data and is suitable for transmission using a communication line.
  • the original image may be transmitted if the communication line can transmit a large amount of data at a time at high speed.
  • step S53 processing of "display original image in preparation simulation window 40" is performed.
  • the original image 41 is displayed based on the original image data received in (step S52).
  • This step S53 becomes unnecessary when only the position recognition image 47 of FIG. 11 is used without using the original image 41.
  • step S54 processing of "converting the original image to the scale of the preparation on the preparation simulation window" is performed.
  • the scale of the position recognition image 47 the number of pixels of the captured image is converted to create a reduced image 42.
  • this step S54 can be performed before transmitting the reduced image data at the requesting terminal 100.
  • step S55 The processing of "Detect the stage position and calculate the observation position on the preparation plate” is performed.
  • step S555 the position of the electric stage 15 when the original image 41 is taken is requested from the requesting terminal 100. If there is a request, the requesting terminal 100 proceeds to step S36 to perform a process of "determining the position of the electric stage and transmitting".
  • step S36 the requesting terminal 100 is requested by the encoder 21 installed on the motorized stage 15 in response to the request of the observation terminal 200. The position of the motorized stage 15 is detected, and the position information is transmitted to the observation side terminal 200 as a response.
  • no encoder 21 is used. If the position information is stored in terms of the number of driving pulses to the noress motor, the position information is calculated from the value and transmitted to the observation side terminal 200. However, when the requesting terminal 200 manages this driving pulse number information, the transmission processing here is unnecessary.
  • step S56 Perform the process of "displaying the reduced image at the position corresponding to the position observed by the real preparation".
  • step S56 from the position of the motorized stage 15 obtained in step S55, the position on the slide 45 of the slide simulation window 40 is changed. Convert to the position. Then, the image obtained in step S54 is displayed as the reduced plane image 42 at the converted position.
  • step S57 the preparation simulation
  • the window 40 or the position recognition image 47 of FIG. 11 displays the mesh outer frame 46 of the mesh capture range.
  • step S59 the screen of the “Blenorat simulation window” is displayed. Change and display the mesh size position on the chart "to change the mesh outer frame 46 to the specified size and position. If there is no instruction to change the mesh position and size (N), the process proceeds to the next step using the already set mesh position and size.
  • step S 60 the process proceeds to “power at which the capture instruction was given by the mesh” in step S 60, and whether or not the capture instruction was given by the mesh.
  • step S 60 the capture instruction is input by the mesh
  • step S63 the capture request by the mesh is transmitted from the observation side terminal 200 to the requesting terminal 100.
  • step S37 start importing by mesh? If there is a request from the observation side terminal 200 in (Step S37),
  • the information of the mesh (magnification, number of divisions, mesh position, etc.) is specified in “Send Mesh Information” in step S61. Observing terminal 200 Requester terminal Send to 100. Upon receiving this mesh information, the requesting terminal 100 performs the process of "change to the magnification specified by the mesh” in step S38. In this step S38, specifically, the magnification of the objective lens 16 is changed by the electric revolver 17 so that the objective lens 1 matches the mesh size (observation range) of the mesh. Select 6.
  • step S39 the requesting terminal 100 proceeds to step S39.
  • the process of "moving the motorized stage to the position where the sample specified by the mesh is taken in” is performed.
  • the observation position of the electric stage 15 is moved to the specified position of the mesh.
  • the requesting terminal 100 acquires the microscope-acquired image in “image acquisition and storage” in step S40, and freezes the acquired microscope-acquired image of the specimen. Images are stored in a storage device such as a data storage device 26 as an image.
  • step S41 the requesting terminal 100 proceeds to step S41.
  • the “mesh capture end power,?” is used to check whether image capture and storage have been completed for all meshes. When all required images have been captured, the mesh capture process ends. .
  • the requesting terminal 100 proceeds to step S42 and performs "image transmission” processing.
  • the image captured by mesh capturing is transmitted to the observation side terminal 200.
  • the terminal 200 on the observation side performs the process of “image reception and storage” in step S62.
  • the transmitted surface images are sequentially stored in the data storage device 26 or the like.
  • step S63 it is confirmed whether or not a series of processing is to be ended in “Is an end instruction input?”
  • a termination instruction Y
  • a termination request is made to the requesting terminal, and a series of processing is terminated. If there is no instruction, the process returns to step S48, and the above series of processing is repeated again.
  • the requesting terminal 100 receives the end request from the observing terminal 200 at "End?" In step S43, and if it is completed, ends a series of processing and ends. If not, return to the step after step S32 and repeat the series of processing again.
  • a window equivalent to the preparation simulation window 40 is displayed on the monitor 10 of the computer 8 of the observation side terminal 200, and The captured image captured from the video camera 18 of the requesting terminal 100 is displayed.
  • Data from computer 1 is transmitted to computer 8 via ISDN line 20 and the same as above on the preparation simulation window 40 of monitor 3 of requesting terminal 100
  • a reduced image obtained by thinning out the pixels of the original image in a preparatory manner and displaying the reduced image is displayed on the surface at the remote observation side terminal 200. From this, the user can operate with the same feeling as operating with the requesting terminal 100, and can operate the actual position of the specimen on the prepara- tor and the observation range. Data can be easily grasped.
  • the observation sample terminal 200 at a remote location Although it was not possible to see the image, it took time to grasp the entire sample. However, the configuration shown in this embodiment makes it easy to view the entire sample from a remote place. In addition to being able to grasp the mesh, the mesh can be set within a desired range.
  • FIG. 12 is a diagram showing a display example of a preparation simulation window 40 used in the present embodiment.
  • FIG. 12 has the same basic configuration as the preparation simulation window 40 shown in the first embodiment.
  • a reduced image 42 is displayed on a pre-order 45 of the preparation simulation window ⁇ 40.
  • the case where the electric stage 15 is moved by using the button 50 shown in FIG. 9 has been described.
  • such an operation takes a long time to move to the target position, and the operability is not good.
  • a method for moving the position to be observed will be described.
  • the reduced image 42 in FIG. 12 shows the state before moving the observation position (before moving).
  • the mouse pointer 60 is moved by the mouse 4 on the reduced image 42.
  • the reduced image 42 is selected using the mouse 4.
  • the reduced image 42 can be dragged (selected) by holding down the button of the mouse 4. Operation
  • the method differs depending on the ring system, any method can be used as long as the reduced image 42 can be selected.
  • the reduced image 4 is moved together with the mouse pointer 60. Make the part of frame 2 moveable.
  • the motorized stage 15 is moved in conjunction with the conversion based on the moving amount on the image. For example, when an observer moves the reduced image 42 in a dragged state on the window 40 and moves it to the position of the reduced image 42 '(after the movement), the electric stage 15 also operates, and the electric stage 15 moves to a position corresponding to the position where the reduced image 42 'is displayed. As a result, the observed image moves with the moving position.
  • the movement range is possible if it is within the movement range of the motorized stage 15, but what is required is within the planar 45. Therefore, the movement range of the mouse pointer 60 within the preparation 45 may be limited.
  • the reduced image 42 and the motorized stage 15 operate in conjunction with the mouse 4, so that the operator can easily see the reduced image 42.
  • the configuration of the device is the same as that described in the first embodiment, and thus the description thereof is omitted.
  • the feature of this embodiment is that when the planar is not fixed in the clean It relates to a positioning method.
  • the preparation plate 53 on the electric stage 15 is connected to the clen-menole a 51 and the clenmen b 52. Therefore, it is fixed by attaching it to each of the Clemmel a 51 and Clen Mell b 52, and the actual preparation 5 3 and the positional relationship on the prepared simulation window 40 are associated with each other.
  • Figure 13 shows the reduced image 42 enlarged.
  • a reduced image 42 is composed of an X-axis reference straight line (cross X) 70 and a Y-axis reference straight line (cross Y) 71 1 2 Display two reference lines. Then, by moving the motorized stage 15, the angle a of the prepared image (in this case, the upper left corner of the prepared plate 53) is moved into the reduced image 42. To be displayed. Further, the motorized stage 15 is moved to a position where the intersection point K of the X-axis reference straight line 70 and the Y-axis reference straight line 71 overlaps the angle a of the prepared image. No, that position. To computer 1 By storing it, it becomes the reference position.
  • the actual prepared The lamp 53 and the positional relation on the preparation simulation window 40 can be associated with each other.
  • the present invention is not limited to the above embodiment.
  • the position recognition image 47 is fixed, and the display position of the reduced image 42 is moved within the position recognition image 47 in conjunction with the movement of the electric stage 15.
  • the display reflects the physical relative positional relationship between the observation position associated with the movement of the motorized stage 15 and the position of each component on the motorized stage 15, for example, a reduced image 42 May be fixed, and the display position of the position recognition image 47 may be moved in accordance with the movement of the electric stage 15.
  • both the reduced image 42 and the position recognition image 47 may be moved on the screen.
  • the whole image of the specimen is predicted (recognized) by moving the reduced image.
  • the reduced image that has been synthesized and displayed is left at the displayed coordinate position. By doing so, the operator can visually recognize the whole image of the specimen.
  • the reduced image moves on the position recognition image.
  • the arrow indicating the moving direction (progression direction) of the reduced image is also displayed on the position recognition image. The operation of button 50 of the operator can be observed This will lead to support.
  • the present invention has been made on the basis of a problem in a microscope image observation system used for pathological observation, but is not particularly limited to use in pathological observation. If the specimen observed in the system described above is something other than cells, or if the image acquisition means can be moved relative to the specimen, it may be in the industrial field, for example, outside the clean room.
  • the present invention can also be applied to the inspection of a specimen to be inspected inside the tall room, for example, an LCD substrate, a wafer substrate, or the like.
  • the present invention not only can the entire image of the specimen be recognized, but also the position and range of the actual observation on the specimen can be easily grasped. You. In addition, the specimen can be easily moved and positioned.
  • the present invention relates to a field of a microscope image observation system used for observation of a specimen and a method of controlling the microscope image observation system for controlling the microscope image observation system. This is effective in the field of technology, and in the technical field of recording media that can be read by a computer on which a control program for controlling this microscope image observation system is recorded.

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  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L'invention concerne une fenêtre de simulation de préparation (40) affichée sur un écran de contrôle (3), dans laquelle apparaît une image originale (41). Des pinces (a43, b44) et une préparation sont elles-mêmes affichés à côté de l'image originale (41). Une image réduite (42) de l'image originale résultant d'un rétrécissement des pixels de l'image originale (41) est affichée sur la préparation (45).
PCT/JP2000/006739 1999-09-29 2000-09-28 Systeme d'observation d'image au microscope, procede de controle, et support enregistre lisible par l'ordinateur a programme de controle enregistre WO2001023938A1 (fr)

Priority Applications (1)

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KR1020017006096A KR20010092741A (ko) 1999-09-29 2000-09-28 현미경화상관찰시스템, 그 제어방법 및 제어프로그램을기록한 컴퓨터에 판독 가능한 기록매체

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JP27700199 1999-09-29
JP11/277001 1999-09-29
JP2000/287626 2000-09-21
JP2000287626A JP4637337B2 (ja) 1999-09-29 2000-09-21 顕微鏡画像観察システム及びその制御方法

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Families Citing this family (13)

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JP3804527B2 (ja) * 2001-12-17 2006-08-02 株式会社ニコン 顕微鏡システム
JP4709492B2 (ja) * 2004-02-20 2011-06-22 オリンパス株式会社 顕微鏡用ステージの転がり部材保持ケーシング位置ずれ補正方法
JP2006126615A (ja) * 2004-10-29 2006-05-18 Nikon Corp 顕微鏡システム
CN100454078C (zh) * 2006-06-22 2009-01-21 北京普利生仪器有限公司 一种全息数字化切片的显微图像制作方法
JP5091460B2 (ja) * 2006-11-08 2012-12-05 株式会社日立国際電気 検査測定装置
CN102707425B (zh) * 2012-06-21 2014-04-16 爱威科技股份有限公司 图像处理方法和装置
JP2014153500A (ja) * 2013-02-07 2014-08-25 Sony Corp 画像取得装置およびステージ制御方法
CN104965302A (zh) * 2015-06-26 2015-10-07 深圳市虚拟现实科技有限公司 增强现实显微镜
AU2016323062A1 (en) * 2015-09-14 2018-04-12 Essenlix Corp. Device and system for analyzing a sample, particularly blood, as well as methods of using the same
CN107492117B (zh) * 2017-07-27 2020-07-28 北京大学 一种基于视频的微颤动功能像成像方法
JP6956591B2 (ja) * 2017-10-30 2021-11-02 株式会社Ｎｏｂｏｒｉ 遠隔病理診断システム及び遠隔病理診断方法
JP7506523B2 (ja) * 2020-05-20 2024-06-26 株式会社エビデント 顕微鏡システム、制御方法、プログラム
JP7506524B2 (ja) * 2020-05-20 2024-06-26 株式会社エビデント 顕微鏡システム、制御方法、プログラム

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293083A2 (fr) * 1987-04-30 1988-11-30 Corabi International Telemetrics, Inc. Système de diagnostic à télétransmission
JPH05241075A (ja) * 1992-03-02 1993-09-21 Olympus Optical Co Ltd 顕微鏡システム
JPH063601A (ja) * 1992-06-22 1994-01-14 Olympus Optical Co Ltd 顕微鏡静止画像伝送システム
JPH063600A (ja) * 1992-06-19 1994-01-14 Olympus Optical Co Ltd 巨視的映像機能を備えた顕微鏡システム
JPH063597A (ja) * 1992-06-22 1994-01-14 Olympus Optical Co Ltd 顕微鏡静止画像観察システム
JPH0651208A (ja) * 1992-07-29 1994-02-25 Olympus Optical Co Ltd 画像遠隔観察システム
JPH06222281A (ja) * 1993-01-22 1994-08-12 Olympus Optical Co Ltd 顕微鏡遠隔観察システム
JPH06275226A (ja) * 1993-03-18 1994-09-30 Sumitomo Metal Ind Ltd 観察位置表示装置
JPH11275572A (ja) * 1998-03-25 1999-10-08 Nikon Corp 顕微鏡画像表示システム及び顕微鏡画像伝送表示システム

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293083A2 (fr) * 1987-04-30 1988-11-30 Corabi International Telemetrics, Inc. Système de diagnostic à télétransmission
JPH05241075A (ja) * 1992-03-02 1993-09-21 Olympus Optical Co Ltd 顕微鏡システム
JPH063600A (ja) * 1992-06-19 1994-01-14 Olympus Optical Co Ltd 巨視的映像機能を備えた顕微鏡システム
JPH063601A (ja) * 1992-06-22 1994-01-14 Olympus Optical Co Ltd 顕微鏡静止画像伝送システム
JPH063597A (ja) * 1992-06-22 1994-01-14 Olympus Optical Co Ltd 顕微鏡静止画像観察システム
JPH0651208A (ja) * 1992-07-29 1994-02-25 Olympus Optical Co Ltd 画像遠隔観察システム
JPH06222281A (ja) * 1993-01-22 1994-08-12 Olympus Optical Co Ltd 顕微鏡遠隔観察システム
JPH06275226A (ja) * 1993-03-18 1994-09-30 Sumitomo Metal Ind Ltd 観察位置表示装置
JPH11275572A (ja) * 1998-03-25 1999-10-08 Nikon Corp 顕微鏡画像表示システム及び顕微鏡画像伝送表示システム

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KR20010092741A (ko) 2001-10-26
TW508452B (en) 2002-11-01

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