WO1999053356A1 - Systeme de microscope - Google Patents
Systeme de microscope Download PDFInfo
- Publication number
- WO1999053356A1 WO1999053356A1 PCT/JP1999/001904 JP9901904W WO9953356A1 WO 1999053356 A1 WO1999053356 A1 WO 1999053356A1 JP 9901904 W JP9901904 W JP 9901904W WO 9953356 A1 WO9953356 A1 WO 9953356A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- wavelength
- light
- laser
- microscope system
- microscope
- Prior art date
Links
- 230000005281 excited state Effects 0.000 claims abstract description 125
- 230000005283 ground state Effects 0.000 claims abstract description 49
- 238000000034 method Methods 0.000 claims abstract description 41
- 230000008569 process Effects 0.000 claims abstract description 39
- 230000005284 excitation Effects 0.000 claims abstract description 29
- 230000003287 optical effect Effects 0.000 claims description 221
- 238000009826 distribution Methods 0.000 claims description 64
- 239000007789 gas Substances 0.000 claims description 35
- 239000013078 crystal Substances 0.000 claims description 32
- 239000004065 semiconductor Substances 0.000 claims description 27
- 230000010355 oscillation Effects 0.000 claims description 25
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 claims description 22
- 239000000298 carbocyanine Substances 0.000 claims description 20
- 230000005684 electric field Effects 0.000 claims description 20
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 claims description 17
- 238000001069 Raman spectroscopy Methods 0.000 claims description 16
- QWYZFXLSWMXLDM-UHFFFAOYSA-M pinacyanol iodide Chemical compound [I-].C1=CC2=CC=CC=C2N(CC)C1=CC=CC1=CC=C(C=CC=C2)C2=[N+]1CC QWYZFXLSWMXLDM-UHFFFAOYSA-M 0.000 claims description 16
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 13
- 230000001143 conditioned effect Effects 0.000 claims description 12
- 229960000956 coumarin Drugs 0.000 claims description 11
- 235000001671 coumarin Nutrition 0.000 claims description 11
- 230000001678 irradiating effect Effects 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 11
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- 229910052757 nitrogen Inorganic materials 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- BCHZICNRHXRCHY-UHFFFAOYSA-N 2h-oxazine Chemical compound N1OC=CC=C1 BCHZICNRHXRCHY-UHFFFAOYSA-N 0.000 claims description 5
- 150000001555 benzenes Chemical class 0.000 claims description 5
- 150000003212 purines Chemical class 0.000 claims description 5
- 239000010453 quartz Substances 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 229940075420 xanthine Drugs 0.000 claims description 5
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims description 4
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims description 4
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims description 4
- FIZIRKROSLGMPL-UHFFFAOYSA-N phenoxazin-1-one Chemical compound C1=CC=C2N=C3C(=O)C=CC=C3OC2=C1 FIZIRKROSLGMPL-UHFFFAOYSA-N 0.000 claims description 4
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 4
- 102100025027 E3 ubiquitin-protein ligase TRIM69 Human genes 0.000 claims description 3
- 101000830203 Homo sapiens E3 ubiquitin-protein ligase TRIM69 Proteins 0.000 claims description 3
- 125000005605 benzo group Chemical group 0.000 claims description 3
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical group C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 claims description 3
- 238000000386 microscopy Methods 0.000 claims description 3
- 239000010979 ruby Substances 0.000 claims description 3
- 229910001750 ruby Inorganic materials 0.000 claims description 3
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims description 3
- PLIKAWJENQZMHA-UHFFFAOYSA-N 4-aminophenol Chemical compound NC1=CC=C(O)C=C1 PLIKAWJENQZMHA-UHFFFAOYSA-N 0.000 claims description 2
- -1 7-octatetraenyl Chemical group 0.000 claims description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims description 2
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 claims description 2
- ZVGCGHVMJAECEG-UHFFFAOYSA-N Chinol Natural products COC1=C(O)C(C)=C(C)C(O)=C1OC ZVGCGHVMJAECEG-UHFFFAOYSA-N 0.000 claims description 2
- ZCQWOFVYLHDMMC-UHFFFAOYSA-N Oxazole Chemical compound C1=COC=N1 ZCQWOFVYLHDMMC-UHFFFAOYSA-N 0.000 claims description 2
- 241000269799 Perca fluviatilis Species 0.000 claims description 2
- 229910052786 argon Inorganic materials 0.000 claims description 2
- RFRXIWQYSOIBDI-UHFFFAOYSA-N benzarone Chemical compound CCC=1OC2=CC=CC=C2C=1C(=O)C1=CC=C(O)C=C1 RFRXIWQYSOIBDI-UHFFFAOYSA-N 0.000 claims description 2
- XZCJVWCMJYNSQO-UHFFFAOYSA-N butyl pbd Chemical compound C1=CC(C(C)(C)C)=CC=C1C1=NN=C(C=2C=CC(=CC=2)C=2C=CC=CC=2)O1 XZCJVWCMJYNSQO-UHFFFAOYSA-N 0.000 claims description 2
- QDLAGTHXVHQKRE-UHFFFAOYSA-N lichenxanthone Natural products COC1=CC(O)=C2C(=O)C3=C(C)C=C(OC)C=C3OC2=C1 QDLAGTHXVHQKRE-UHFFFAOYSA-N 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical compound C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 2
- CVAVMIODJQHEEH-UHFFFAOYSA-O rhodamine B(1+) Chemical compound C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O CVAVMIODJQHEEH-UHFFFAOYSA-O 0.000 claims 2
- OYVFJKVYVDYPFV-UHFFFAOYSA-M 3-ethyl-2-[7-(3-ethyl-1,3-benzothiazol-3-ium-2-yl)hepta-2,4,6-trienylidene]-1,3-benzothiazole;iodide Chemical compound [I-].S1C2=CC=CC=C2[N+](CC)=C1/C=C/C=C/C=C/C=C1/N(CC)C2=CC=CC=C2S1 OYVFJKVYVDYPFV-UHFFFAOYSA-M 0.000 claims 1
- MRUWJENAYHTDQG-UHFFFAOYSA-N 4H-pyran Chemical compound C1C=COC=C1 MRUWJENAYHTDQG-UHFFFAOYSA-N 0.000 claims 1
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 claims 1
- JGLWGLKNDHZFAP-UHFFFAOYSA-N C3-thiacarbocyanine cation Chemical compound S1C2=CC=CC=C2[N+](CC)=C1C=CC=C1N(CC)C2=CC=CC=C2S1 JGLWGLKNDHZFAP-UHFFFAOYSA-N 0.000 claims 1
- CLDZYSUDOQXJOU-UHFFFAOYSA-M C5-oxacyanine Chemical compound [I-].O1C2=CC=CC=C2[N+](CC)=C1C=CC=CC=C1N(CC)C2=CC=CC=C2O1 CLDZYSUDOQXJOU-UHFFFAOYSA-M 0.000 claims 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 claims 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid group Chemical group C(C1=CC=CC=C1)(=O)O WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims 1
- 150000002148 esters Chemical class 0.000 claims 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 1
- 239000004744 fabric Substances 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 230000007423 decrease Effects 0.000 abstract description 4
- 238000004043 dyeing Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 106
- 238000010586 diagram Methods 0.000 description 47
- 238000010521 absorption reaction Methods 0.000 description 37
- 230000006870 function Effects 0.000 description 30
- 239000011521 glass Substances 0.000 description 22
- 239000002245 particle Substances 0.000 description 15
- 239000010408 film Substances 0.000 description 12
- 238000005259 measurement Methods 0.000 description 12
- 238000005516 engineering process Methods 0.000 description 11
- 230000010287 polarization Effects 0.000 description 11
- 239000000975 dye Substances 0.000 description 10
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 238000002073 fluorescence micrograph Methods 0.000 description 8
- 230000001629 suppression Effects 0.000 description 8
- 230000005540 biological transmission Effects 0.000 description 7
- 238000003384 imaging method Methods 0.000 description 7
- 238000004776 molecular orbital Methods 0.000 description 7
- 230000007704 transition Effects 0.000 description 6
- 101100412856 Mus musculus Rhod gene Proteins 0.000 description 5
- 101100242191 Tetraodon nigroviridis rho gene Proteins 0.000 description 5
- 239000012472 biological sample Substances 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 239000011248 coating agent Substances 0.000 description 5
- 238000000576 coating method Methods 0.000 description 5
- 230000004907 flux Effects 0.000 description 5
- ORUIBWPALBXDOA-UHFFFAOYSA-L magnesium fluoride Chemical compound [F-].[F-].[Mg+2] ORUIBWPALBXDOA-UHFFFAOYSA-L 0.000 description 5
- 229910001635 magnesium fluoride Inorganic materials 0.000 description 5
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 5
- 229910052753 mercury Inorganic materials 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000006059 cover glass Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 210000001747 pupil Anatomy 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000004305 biphenyl Substances 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000000799 fluorescence microscopy Methods 0.000 description 3
- 238000002189 fluorescence spectrum Methods 0.000 description 3
- 230000014509 gene expression Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 241001247414 Couma Species 0.000 description 2
- WTZYXHFDCKUDNL-UHFFFAOYSA-N benzene;pyrazine Chemical compound C1=CC=CC=C1.C1=CN=CC=N1 WTZYXHFDCKUDNL-UHFFFAOYSA-N 0.000 description 2
- IOJUPLGTWVMSFF-UHFFFAOYSA-N benzothiazole Chemical compound C1=CC=C2SC=NC2=C1 IOJUPLGTWVMSFF-UHFFFAOYSA-N 0.000 description 2
- WUKWITHWXAAZEY-UHFFFAOYSA-L calcium difluoride Chemical compound [F-].[F-].[Ca+2] WUKWITHWXAAZEY-UHFFFAOYSA-L 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 125000003636 chemical group Chemical group 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 239000010436 fluorite Substances 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- TZMSYXZUNZXBOL-UHFFFAOYSA-N 10H-phenoxazine Chemical compound C1=CC=C2NC3=CC=CC=C3OC2=C1 TZMSYXZUNZXBOL-UHFFFAOYSA-N 0.000 description 1
- PDQRQJVPEFGVRK-UHFFFAOYSA-N 2,1,3-benzothiadiazole Chemical compound C1=CC=CC2=NSN=C21 PDQRQJVPEFGVRK-UHFFFAOYSA-N 0.000 description 1
- 241000406668 Loxodonta cyclotis Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- PJANXHGTPQOBST-VAWYXSNFSA-N Stilbene Natural products C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- BJQHLKABXJIVAM-UHFFFAOYSA-N bis(2-ethylhexyl) phthalate Chemical compound CCCCC(CC)COC(=O)C1=CC=CC=C1C(=O)OCC(CC)CCCC BJQHLKABXJIVAM-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- VYXSBFYARXAAKO-WTKGSRSZSA-N chembl402140 Chemical compound Cl.C1=2C=C(C)C(NCC)=CC=2OC2=C\C(=N/CC)C(C)=CC2=C1C1=CC=CC=C1C(=O)OCC VYXSBFYARXAAKO-WTKGSRSZSA-N 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- GZTMNDOZYLMFQE-UHFFFAOYSA-N coumarin 500 Chemical compound FC(F)(F)C1=CC(=O)OC2=CC(NCC)=CC=C21 GZTMNDOZYLMFQE-UHFFFAOYSA-N 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical class [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 210000001061 forehead Anatomy 0.000 description 1
- 238000005338 heat storage Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000012576 optical tweezer Methods 0.000 description 1
- 101150032584 oxy-4 gene Proteins 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 229940083251 peripheral vasodilators purine derivative Drugs 0.000 description 1
- 238000006552 photochemical reaction Methods 0.000 description 1
- 230000001443 photoexcitation Effects 0.000 description 1
- 208000017983 photosensitivity disease Diseases 0.000 description 1
- 231100000434 photosensitization Toxicity 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical compound C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 1
- 235000021286 stilbenes Nutrition 0.000 description 1
- 125000005504 styryl group Chemical group 0.000 description 1
- 229910052718 tin Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/636—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited using an arrangement of pump beam and probe beam; using the measurement of optical non-linear properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6445—Measuring fluorescence polarisation
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0056—Optical details of the image generation based on optical coherence, e.g. phase-contrast arrangements, interference arrangements
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0072—Optical details of the image generation details concerning resolution or correction, including general design of CSOM objectives
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
- G21K—TECHNIQUES FOR HANDLING PARTICLES OR IONISING RADIATION NOT OTHERWISE PROVIDED FOR; IRRADIATION DEVICES; GAMMA RAY OR X-RAY MICROSCOPES
- G21K7/00—Gamma- or X-ray microscopes
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19980759.0A DE19980759B8 (de) | 1998-04-09 | 1999-04-09 | Mikroskopsystem |
US09/445,389 US6667830B1 (en) | 1998-04-09 | 1999-04-09 | Super-resolution microscope system and method for illumination |
DE19980759T DE19980759T1 (de) | 1998-04-09 | 1999-04-09 | Mikroskopsystem |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10/97924 | 1998-04-09 | ||
JP09792498A JP3350442B2 (ja) | 1998-04-09 | 1998-04-09 | 顕微鏡システム |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1999053356A1 true WO1999053356A1 (fr) | 1999-10-21 |
Family
ID=14205245
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP1999/001904 WO1999053356A1 (fr) | 1998-04-09 | 1999-04-09 | Systeme de microscope |
Country Status (4)
Families Citing this family (81)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69830598T2 (de) | 1997-01-31 | 2006-05-18 | The Horticulture And Food Research Institute Of New Zealand Limited | Optische vorrichtung und methode |
US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
CA2338194C (en) | 1998-07-30 | 2013-09-10 | Edward L. Squires | Equine system for non-surgical artificial insemination |
US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
JP4551528B2 (ja) * | 2000-03-23 | 2010-09-29 | オリンパス株式会社 | 二重共鳴吸収顕微鏡 |
US6844963B2 (en) * | 2000-03-23 | 2005-01-18 | Olympus Optical Co., Ltd. | Double-resonance-absorption microscope |
CA2408939C (en) | 2000-05-09 | 2011-11-08 | Xy, Inc. | High purity x-chromosome bearing and y-chromosome bearing populations of spermatozoa |
AU2002220018A1 (en) | 2000-11-29 | 2002-06-11 | Colorado State University | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
JP2003066338A (ja) * | 2001-08-28 | 2003-03-05 | Olympus Optical Co Ltd | 顕微鏡 |
US6859313B2 (en) | 2001-03-23 | 2005-02-22 | Japan Science & Technology Corporation | Super resolution microscope |
DE10120425C2 (de) * | 2001-04-26 | 2003-12-18 | Leica Microsystems | Scanmikroskop |
MXPA05001100A (es) | 2002-08-01 | 2005-04-28 | Xy Inc | Sistema de separacion de baja presion para celulas de esperma. |
US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
WO2004017041A2 (en) | 2002-08-15 | 2004-02-26 | Xy, Inc. | High resolution flow cytometer |
DE10241261A1 (de) * | 2002-09-06 | 2004-03-18 | Leica Microsystems (Schweiz) Ag | Schutzbeleuchtung für Operationsmikroskope |
US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
AU2002347689A1 (en) * | 2002-09-27 | 2004-04-19 | Wilson O. Garcia | Two-color (two-photon) excitation with focused excitation beams and a raman shifter |
US7432036B2 (en) * | 2002-10-07 | 2008-10-07 | Fujifilm Corporation | Non-resonant two-photon absorbing material, non-resonant two-photon emitting material, and method for inducing absorption or generating emission of non-resonant two photons by using the material |
DK2309245T3 (en) | 2003-03-28 | 2016-01-04 | Inguran Llc | Methods for providing sex-sorted animal semen |
EP1616216B1 (de) * | 2003-04-13 | 2018-11-07 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Räumlich hochauflösendes abbilden |
NZ544103A (en) | 2003-05-15 | 2010-10-29 | Xy Llc | Efficient haploid cell sorting for flow cytometer systems |
JP4198086B2 (ja) * | 2003-06-25 | 2008-12-17 | オリンパス株式会社 | 蛍光観察用装置 |
DE10340965A1 (de) * | 2003-09-05 | 2005-03-24 | Leica Microsystems Heidelberg Gmbh | Rastermikroskop |
WO2005029149A1 (de) * | 2003-09-25 | 2005-03-31 | Leica Microsystems Cms Gmbh | Mikroskop mit evaneszenter beleuchtung |
DE10347712A1 (de) * | 2003-10-14 | 2005-05-12 | Leica Microsystems | Rastermikroskop |
JP3993553B2 (ja) | 2003-10-15 | 2007-10-17 | 独立行政法人科学技術振興機構 | 3次元分析装置 |
US7869469B1 (en) * | 2004-03-19 | 2011-01-11 | University Corporation For Atmospheric Research | Raman cell for high power applications |
EP2151243B1 (en) | 2004-03-29 | 2012-10-24 | Inguran, LLC | Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations |
US7221449B2 (en) * | 2004-06-28 | 2007-05-22 | Applera Corporation | Apparatus for assaying fluorophores in a biological sample |
DE102004032953B4 (de) * | 2004-07-07 | 2008-02-07 | Leica Microsystems Cms Gmbh | Optische Vorrichtung und Rastermikroskop mit einer fokussierenden Optik |
CA2574499C (en) | 2004-07-22 | 2016-11-29 | Monsanto Technology Llc | Process for enriching a population of sperm cells |
JP2006047912A (ja) * | 2004-08-09 | 2006-02-16 | Olympus Corp | 超解像顕微鏡 |
JP4652801B2 (ja) * | 2004-12-22 | 2011-03-16 | オリンパス株式会社 | 透過照明装置、それを備えた顕微鏡、及び透過照明方法 |
US7491943B2 (en) * | 2005-01-13 | 2009-02-17 | Whitehead Institute For Biomedical Research | Method and apparatus for UV imaging |
US7969650B2 (en) * | 2005-04-28 | 2011-06-28 | The Board Of Trustees Of The University Of Illinois | Multiplex near-field microscopy with diffractive elements |
GB0509773D0 (en) * | 2005-05-13 | 2005-06-22 | Iti Scotland Ltd | An optical system for laser excitation and collection of fluorescence emissions |
EP2453240B1 (en) * | 2005-05-23 | 2016-12-28 | Harald F. Hess | Optical microscopy with phototransformable optical labels |
EP1907826B2 (de) * | 2005-07-22 | 2020-11-25 | Carl Zeiss MicroImaging GmbH | Auflösungsgesteigerte lumineszenz-mikroskopie |
US7619732B2 (en) * | 2006-03-01 | 2009-11-17 | Leica Microsystems Cms Gmbh | Method and microscope for high spatial resolution examination of samples |
DE102006009832B4 (de) * | 2006-03-01 | 2013-07-04 | Leica Microsystems Cms Gmbh | Verfahren und Mikroskop zur räumlich hochauflösenden Untersuchung von Proben |
DE102006021317B3 (de) * | 2006-05-06 | 2007-10-11 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren und Fluoreszenzlichtmikroskop zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
JP4912738B2 (ja) * | 2006-05-11 | 2012-04-11 | オリンパス株式会社 | レーザ走査型顕微鏡 |
FR2902202B1 (fr) * | 2006-06-08 | 2008-09-26 | Centre Nat Rech Scient | Microscope confocal interferometrique |
JP2008026471A (ja) * | 2006-07-19 | 2008-02-07 | Olympus Corp | 試料観察方法および顕微鏡 |
JP2008058003A (ja) * | 2006-08-29 | 2008-03-13 | Olympus Corp | 顕微鏡 |
JP5065668B2 (ja) * | 2006-12-26 | 2012-11-07 | オリンパス株式会社 | 顕微鏡法および顕微鏡 |
US8906354B2 (en) | 2007-02-28 | 2014-12-09 | Bruker Biospin Corporation | Loaded latex optical molecular imaging probes containing lipophilic large stokes shift dyes |
US8017104B2 (en) * | 2007-02-28 | 2011-09-13 | Carestream Health, Inc. | Large stoke shift dye used for optical imaging |
EP2107363B1 (en) * | 2008-03-31 | 2012-07-11 | Deutsches Krebsforschungszentrum | Method of fluorescence-microscopically imaging a structure in a sample with high three-dimensional spatial resolution |
US8174692B2 (en) * | 2008-05-21 | 2012-05-08 | Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. | High spatial resolution imaging of a structure of interest in a specimen |
DE102008024568A1 (de) * | 2008-05-21 | 2009-12-03 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zum räumlich hochauflösenden Abbilden einer interessierenden Struktur einer Probe |
DE102008034008B4 (de) * | 2008-07-21 | 2010-07-01 | Carl Zeiss Surgical Gmbh | Filtersatz zur Beobachtung von Fluoreszenzstrahlung in biologischem Gewebe |
KR101031779B1 (ko) * | 2008-11-20 | 2011-04-29 | 한국표준과학연구원 | 다중광노출법 기반 초고분해능 리소그래피 장치 및 방법 |
JP5453849B2 (ja) * | 2009-03-06 | 2014-03-26 | コニカミノルタ株式会社 | 光出射プローブ、光出射装置、生体情報検出装置およびブレイン・マシン・インターフェース |
EP2454569B1 (en) | 2009-07-10 | 2014-12-24 | The U.S.A. As Represented By The Secretary, Department Of Health And Human Services | Emission detection for multi-photon microscopy |
JP5639182B2 (ja) * | 2009-11-02 | 2014-12-10 | オリンパス株式会社 | ビームスプリッタ装置、光源装置および走査型観察装置 |
JP5484879B2 (ja) * | 2009-12-11 | 2014-05-07 | オリンパス株式会社 | 超解像顕微鏡 |
US8717560B2 (en) * | 2010-05-04 | 2014-05-06 | University Of Maine System Board Of Trustees | Ring grating spectrometer |
JP5771422B2 (ja) | 2010-06-17 | 2015-08-26 | オリンパス株式会社 | 顕微鏡 |
DE102010033825B9 (de) | 2010-08-09 | 2024-04-18 | Carl Zeiss Meditec Ag | Fluoreszenzbeobachtungssystem und Filtersatz |
JP5655434B2 (ja) * | 2010-08-30 | 2015-01-21 | 株式会社ニコン | 観察装置及び観察方法 |
JP5703126B2 (ja) * | 2010-09-30 | 2015-04-15 | 富士フイルム株式会社 | 生体分子検出装置および生体分子検出方法 |
JP2013076770A (ja) | 2011-09-29 | 2013-04-25 | Olympus Corp | 非線形光学顕微鏡および非線形光学顕微鏡法 |
EP2581775A1 (en) * | 2011-10-14 | 2013-04-17 | Astrium Limited | Resonator Optimisation |
JP2014098690A (ja) * | 2012-10-16 | 2014-05-29 | Canon Inc | 校正装置、校正方法及び計測装置 |
DE102013100174A1 (de) * | 2013-01-09 | 2014-07-10 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zum räumlich hochaufgelösten Abbilden einer einen Luminophor aufweisenden Struktur einer Probe |
JP2014182239A (ja) | 2013-03-19 | 2014-09-29 | Olympus Corp | 超解像顕微鏡 |
JP2015025758A (ja) * | 2013-07-26 | 2015-02-05 | Hoya株式会社 | 基板検査方法、基板製造方法および基板検査装置 |
JP6234105B2 (ja) | 2013-08-05 | 2017-11-22 | オリンパス株式会社 | 超解像顕微鏡 |
KR101601365B1 (ko) * | 2013-08-07 | 2016-03-09 | 연세대학교 산학협력단 | 향상된 분해능을 가지는 광학 영상 장치 |
KR20150024547A (ko) * | 2013-08-27 | 2015-03-09 | 동우 화인켐 주식회사 | 광 루미네선스 코팅 조성물 및 이를 이용한 광 루미네선스 필름 |
GB2520554B (en) | 2013-11-26 | 2019-03-27 | Cairn Res Limited | Improved Optical arrangement for digital micromirror device |
KR20150109854A (ko) * | 2014-03-21 | 2015-10-02 | 동우 화인켐 주식회사 | 광 루미네선스 코팅 조성물 및 이를 이용한 광 루미네선스 필름 |
JP2016012114A (ja) * | 2014-06-02 | 2016-01-21 | オリンパス株式会社 | 照明装置、これを有する顕微鏡装置及び顕微鏡観察方法 |
JP2016052672A (ja) * | 2014-09-04 | 2016-04-14 | 株式会社ディスコ | レーザー加工装置 |
JP6355515B2 (ja) * | 2014-10-07 | 2018-07-11 | 浜松ホトニクス株式会社 | 光照射装置及び光照射方法 |
US10395888B2 (en) * | 2017-03-30 | 2019-08-27 | The Regents Of The University Of California | Optical-cavity based ponderomotive phase plate for transmission electron microscopy |
US11221473B2 (en) * | 2017-04-06 | 2022-01-11 | Clemson University Research Foundation | Pulse-illuminated edge deduction microscopy |
JP2019117217A (ja) | 2017-12-26 | 2019-07-18 | オリンパス株式会社 | 超解像顕微鏡 |
DE102018132212B4 (de) * | 2018-12-14 | 2020-09-17 | Leica Microsystems Cms Gmbh | Verfahren und Mikroskop zur hochaufgelösten lichtmikroskopischen Abbildung einer Probe |
Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60131428A (ja) * | 1983-12-21 | 1985-07-13 | Komatsu Ltd | カ−ス分光装置 |
JPS61111530A (ja) * | 1984-11-06 | 1986-05-29 | Canon Inc | 露光装置 |
JPH0291545A (ja) * | 1987-09-17 | 1990-03-30 | American Teleph & Telegr Co <Att> | 光学トラップ装置 |
JPH04171415A (ja) * | 1990-11-02 | 1992-06-18 | Nikon Corp | 長焦点深度高分解能照射光学系 |
JPH05224128A (ja) * | 1992-02-14 | 1993-09-03 | Olympus Optical Co Ltd | 走査型顕微鏡 |
JPH06180314A (ja) * | 1991-12-05 | 1994-06-28 | Miles Inc | 試薬組成物及びその全血中の網状赤血球の同定と特性表示のための使用 |
JPH07196930A (ja) * | 1993-07-12 | 1995-08-01 | Molecular Probes Inc | 環式置換−非対称シアニン染料 |
JPH0815156A (ja) * | 1993-06-03 | 1996-01-19 | Hamamatsu Photonics Kk | レーザスキャン光学系及びレーザスキャン光学装置 |
JPH08248200A (ja) * | 1995-03-15 | 1996-09-27 | Res Dev Corp Of Japan | 多波長光光学顕微鏡 |
JPH10142151A (ja) * | 1996-11-13 | 1998-05-29 | Kagaku Gijutsu Shinko Jigyodan | 光学顕微鏡 |
JPH1195120A (ja) * | 1997-09-19 | 1999-04-09 | Olympus Optical Co Ltd | 顕微鏡の観察方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5245619A (en) * | 1992-01-03 | 1993-09-14 | Kronberg James W | Generation of low-divergence laser beams |
EP0627643B1 (en) * | 1993-06-03 | 1999-05-06 | Hamamatsu Photonics K.K. | Laser scanning optical system using axicon |
DE4331570C2 (de) * | 1993-08-17 | 1996-10-24 | Hell Stefan | Verfahren zum optischen Anregen einer Probe |
US5866911A (en) * | 1994-07-15 | 1999-02-02 | Baer; Stephen C. | Method and apparatus for improving resolution in scanned optical system |
JP3164989B2 (ja) * | 1994-12-28 | 2001-05-14 | 科学技術振興事業団 | 試料観察方法および多波長光光学顕微鏡 |
-
1998
- 1998-04-09 JP JP09792498A patent/JP3350442B2/ja not_active Expired - Fee Related
-
1999
- 1999-04-09 US US09/445,389 patent/US6667830B1/en not_active Expired - Lifetime
- 1999-04-09 DE DE19980759T patent/DE19980759T1/de active Granted
- 1999-04-09 WO PCT/JP1999/001904 patent/WO1999053356A1/ja active Application Filing
- 1999-04-09 DE DE19980759.0A patent/DE19980759B8/de not_active Expired - Lifetime
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60131428A (ja) * | 1983-12-21 | 1985-07-13 | Komatsu Ltd | カ−ス分光装置 |
JPS61111530A (ja) * | 1984-11-06 | 1986-05-29 | Canon Inc | 露光装置 |
JPH0291545A (ja) * | 1987-09-17 | 1990-03-30 | American Teleph & Telegr Co <Att> | 光学トラップ装置 |
JPH04171415A (ja) * | 1990-11-02 | 1992-06-18 | Nikon Corp | 長焦点深度高分解能照射光学系 |
JPH06180314A (ja) * | 1991-12-05 | 1994-06-28 | Miles Inc | 試薬組成物及びその全血中の網状赤血球の同定と特性表示のための使用 |
JPH05224128A (ja) * | 1992-02-14 | 1993-09-03 | Olympus Optical Co Ltd | 走査型顕微鏡 |
JPH0815156A (ja) * | 1993-06-03 | 1996-01-19 | Hamamatsu Photonics Kk | レーザスキャン光学系及びレーザスキャン光学装置 |
JPH07196930A (ja) * | 1993-07-12 | 1995-08-01 | Molecular Probes Inc | 環式置換−非対称シアニン染料 |
JPH08248200A (ja) * | 1995-03-15 | 1996-09-27 | Res Dev Corp Of Japan | 多波長光光学顕微鏡 |
JPH10142151A (ja) * | 1996-11-13 | 1998-05-29 | Kagaku Gijutsu Shinko Jigyodan | 光学顕微鏡 |
JPH1195120A (ja) * | 1997-09-19 | 1999-04-09 | Olympus Optical Co Ltd | 顕微鏡の観察方法 |
Also Published As
Publication number | Publication date |
---|---|
JP3350442B2 (ja) | 2002-11-25 |
JP2001100102A (ja) | 2001-04-13 |
DE19980759T1 (de) | 2001-10-18 |
DE19980759B8 (de) | 2016-12-22 |
US6667830B1 (en) | 2003-12-23 |
DE19980759B3 (de) | 2016-10-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO1999053356A1 (fr) | Systeme de microscope | |
US6844963B2 (en) | Double-resonance-absorption microscope | |
JP5526191B2 (ja) | 光測定装置 | |
US6633432B2 (en) | Optical device and a microscope | |
JP5771422B2 (ja) | 顕微鏡 | |
WO2008026435A1 (en) | Microscope | |
JP2009145242A (ja) | 光測定装置 | |
JP2018146602A (ja) | 観察装置 | |
JP2006023745A (ja) | 位相フィルタ、光学装置及びラスタ顕微鏡 | |
JP3164989B2 (ja) | 試料観察方法および多波長光光学顕微鏡 | |
US20070183029A1 (en) | Microscope and its optical controlling method | |
CN205719974U (zh) | 一种基于超连续谱光源的多色荧光成像系统 | |
JP4551528B2 (ja) | 二重共鳴吸収顕微鏡 | |
JP2006047912A (ja) | 超解像顕微鏡 | |
Spring | Fluorescence microscopy | |
JP3993553B2 (ja) | 3次元分析装置 | |
JP3020453B2 (ja) | 光学顕微鏡 | |
JP2002202459A (ja) | 暗視野落射顕微鏡 | |
JP2004309458A (ja) | 時間分解蛍光顕微鏡 | |
US10955649B2 (en) | Method of STED microscopy | |
JP2008057997A (ja) | 化学発光試料の観察方法および顕微鏡 | |
JP2001272343A (ja) | 二重共鳴吸収顕微鏡 | |
JP4614495B2 (ja) | 二重共鳴吸収顕微鏡 | |
JP5086765B2 (ja) | 顕微鏡 | |
JP2004109368A (ja) | 顕微鏡 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): DE US |
|
WWE | Wipo information: entry into national phase |
Ref document number: 09445389 Country of ref document: US |
|
RET | De translation (de og part 6b) |
Ref document number: 19980759 Country of ref document: DE Date of ref document: 20011018 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 19980759 Country of ref document: DE |