DE19980759T1 - Mikroskopsystem - Google Patents

Mikroskopsystem

Info

Publication number
DE19980759T1
DE19980759T1 DE19980759T DE19980759T DE19980759T1 DE 19980759 T1 DE19980759 T1 DE 19980759T1 DE 19980759 T DE19980759 T DE 19980759T DE 19980759 T DE19980759 T DE 19980759T DE 19980759 T1 DE19980759 T1 DE 19980759T1
Authority
DE
Germany
Prior art keywords
microscope system
microscope
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
DE19980759T
Other languages
English (en)
Other versions
DE19980759B8 (de
DE19980759B3 (de
Inventor
Yoshinori Iketaki
Masaaki Fjii
Takashige Omatsu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Japan Science and Technology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science and Technology Corp filed Critical Japan Science and Technology Corp
Publication of DE19980759T1 publication Critical patent/DE19980759T1/de
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/636—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited using an arrangement of pump beam and probe beam; using the measurement of optical non-linear properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0056—Optical details of the image generation based on optical coherence, e.g. phase-contrast arrangements, interference arrangements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0072—Optical details of the image generation details concerning resolution or correction, including general design of CSOM objectives
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
    • G21K—TECHNIQUES FOR HANDLING PARTICLES OR IONISING RADIATION NOT OTHERWISE PROVIDED FOR; IRRADIATION DEVICES; GAMMA RAY OR X-RAY MICROSCOPES
    • G21K7/00—Gamma- or X-ray microscopes
DE19980759T 1998-04-09 1999-04-09 Mikroskopsystem Granted DE19980759T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP09792498A JP3350442B2 (ja) 1998-04-09 1998-04-09 顕微鏡システム
PCT/JP1999/001904 WO1999053356A1 (fr) 1998-04-09 1999-04-09 Systeme de microscope

Publications (1)

Publication Number Publication Date
DE19980759T1 true DE19980759T1 (de) 2001-10-18

Family

ID=14205245

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19980759T Granted DE19980759T1 (de) 1998-04-09 1999-04-09 Mikroskopsystem
DE19980759.0A Expired - Lifetime DE19980759B8 (de) 1998-04-09 1999-04-09 Mikroskopsystem

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE19980759.0A Expired - Lifetime DE19980759B8 (de) 1998-04-09 1999-04-09 Mikroskopsystem

Country Status (4)

Country Link
US (1) US6667830B1 (de)
JP (1) JP3350442B2 (de)
DE (2) DE19980759T1 (de)
WO (1) WO1999053356A1 (de)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7812967B2 (en) 2006-12-26 2010-10-12 Olympus Corporation Microscopy method and microscope
DE102010033825A1 (de) * 2010-08-09 2012-02-09 Carl Zeiss Meditec Ag Fluoreszenzbeobachtungssystem und Filtersatz
US8699132B2 (en) 2010-06-17 2014-04-15 Olympus Corporation Ultra-high resolution microscope
US9310595B2 (en) 2013-03-19 2016-04-12 Olympus Corporation Super-resolution microscope

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AR018234A1 (es) 1998-07-30 2001-11-14 Xy Inc Metodo para producir una muestra para inseminacion de flujo de equinos y disposicion de citometria de flujo.
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US6844963B2 (en) * 2000-03-23 2005-01-18 Olympus Optical Co., Ltd. Double-resonance-absorption microscope
JP4551528B2 (ja) * 2000-03-23 2010-09-29 オリンパス株式会社 二重共鳴吸収顕微鏡
RU2297198C2 (ru) 2000-05-09 2007-04-20 Кси, Инк. Способ разделения клеток спермы, несущих x-хромосому, и клеток спермы, несущих y-хромосому
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
WO2002043574A2 (en) 2000-11-29 2002-06-06 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
JP2003066338A (ja) * 2001-08-28 2003-03-05 Olympus Optical Co Ltd 顕微鏡
US6859313B2 (en) 2001-03-23 2005-02-22 Japan Science & Technology Corporation Super resolution microscope
DE10120425C2 (de) * 2001-04-26 2003-12-18 Leica Microsystems Scanmikroskop
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
JP4595067B2 (ja) 2002-08-01 2010-12-08 エックスワイ，エルエルシー 低圧精子細胞分離システム
JP2005535346A (ja) 2002-08-15 2005-11-24 エックスワイ，インコーポレイテッド 高分解能フローサイトメーター
DE10241261A1 (de) * 2002-09-06 2004-03-18 Leica Microsystems (Schweiz) Ag Schutzbeleuchtung für Operationsmikroskope
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
AU2002347689A1 (en) * 2002-09-27 2004-04-19 Wilson O. Garcia Two-color (two-photon) excitation with focused excitation beams and a raman shifter
US7432036B2 (en) * 2002-10-07 2008-10-07 Fujifilm Corporation Non-resonant two-photon absorbing material, non-resonant two-photon emitting material, and method for inducing absorption or generating emission of non-resonant two photons by using the material
BRPI0408857B1 (pt) 2003-03-28 2018-09-11 Inguran Llc aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo
WO2004090617A2 (de) * 2003-04-13 2004-10-21 Max-Planck-Gesselschaft Zur Förderung Der Wissenschaften E.V. Räumlich hochauflösendes abbilden
AU2004242121B2 (en) 2003-05-15 2010-06-24 Xy, Llc. Efficient haploid cell sorting for flow cytometer systems
JP4198086B2 (ja) * 2003-06-25 2008-12-17 オリンパス株式会社 蛍光観察用装置
DE10340965A1 (de) * 2003-09-05 2005-03-24 Leica Microsystems Heidelberg Gmbh Rastermikroskop
WO2005029149A1 (de) * 2003-09-25 2005-03-31 Leica Microsystems Cms Gmbh Mikroskop mit evaneszenter beleuchtung
DE10347712A1 (de) * 2003-10-14 2005-05-12 Leica Microsystems Rastermikroskop
JP3993553B2 (ja) 2003-10-15 2007-10-17 独立行政法人科学技術振興機構 ３次元分析装置
US7869469B1 (en) * 2004-03-19 2011-01-11 University Corporation For Atmospheric Research Raman cell for high power applications
BRPI0509485A (pt) 2004-03-29 2007-09-11 Monsanto Technology Llc suspensões de esperma para uso em inseminação
US7221449B2 (en) * 2004-06-28 2007-05-22 Applera Corporation Apparatus for assaying fluorophores in a biological sample
DE102004032953B4 (de) * 2004-07-07 2008-02-07 Leica Microsystems Cms Gmbh Optische Vorrichtung und Rastermikroskop mit einer fokussierenden Optik
EP2269617B1 (de) 2004-07-22 2016-04-27 Inguran, LLC Spermasuspensionen zur Sortierung in X- oder Y-Chromosomen-tragende angereicherte Populationen
JP2006047912A (ja) 2004-08-09 2006-02-16 Olympus Corp 超解像顕微鏡
JP4652801B2 (ja) * 2004-12-22 2011-03-16 オリンパス株式会社 透過照明装置、それを備えた顕微鏡、及び透過照明方法
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JP5453849B2 (ja) * 2009-03-06 2014-03-26 コニカミノルタ株式会社 光出射プローブ、光出射装置、生体情報検出装置およびブレイン・マシン・インターフェース
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JP6234105B2 (ja) 2013-08-05 2017-11-22 オリンパス株式会社 超解像顕微鏡
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JP2016012114A (ja) * 2014-06-02 2016-01-21 オリンパス株式会社 照明装置、これを有する顕微鏡装置及び顕微鏡観察方法
JP2016052672A (ja) * 2014-09-04 2016-04-14 株式会社ディスコ レーザー加工装置
JP6355515B2 (ja) * 2014-10-07 2018-07-11 浜松ホトニクス株式会社 光照射装置及び光照射方法
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7812967B2 (en) 2006-12-26 2010-10-12 Olympus Corporation Microscopy method and microscope
US8699132B2 (en) 2010-06-17 2014-04-15 Olympus Corporation Ultra-high resolution microscope
DE102010033825A1 (de) * 2010-08-09 2012-02-09 Carl Zeiss Meditec Ag Fluoreszenzbeobachtungssystem und Filtersatz
US8730601B2 (en) 2010-08-09 2014-05-20 Carl Zeiss Meditec Ag Fluorescence observation system and set of filters
DE102010033825B4 (de) 2010-08-09 2023-11-09 Carl Zeiss Meditec Ag Fluoreszenzbeobachtungssystem und Filtersatz
DE102010033825B9 (de) 2010-08-09 2024-04-18 Carl Zeiss Meditec Ag Fluoreszenzbeobachtungssystem und Filtersatz
US9310595B2 (en) 2013-03-19 2016-04-12 Olympus Corporation Super-resolution microscope

Also Published As

Publication number Publication date
US6667830B1 (en) 2003-12-23
JP3350442B2 (ja) 2002-11-25
DE19980759B8 (de) 2016-12-22
DE19980759B3 (de) 2016-10-06
WO1999053356A1 (fr) 1999-10-21
JP2001100102A (ja) 2001-04-13

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