US20110059919A1 - Antiviral composition comprising a sulfated polysaccharide - Google Patents

Antiviral composition comprising a sulfated polysaccharide Download PDF

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US20110059919A1
US20110059919A1 US12/673,145 US67314508A US2011059919A1 US 20110059919 A1 US20110059919 A1 US 20110059919A1 US 67314508 A US67314508 A US 67314508A US 2011059919 A1 US2011059919 A1 US 2011059919A1
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carrageenan
iota
virus
cells
composition
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Andreas Grassauer
Eva Prieschl-Grassauer
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Marinomed Biotech AG
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/731—Carrageenans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/737—Sulfated polysaccharides, e.g. chondroitin sulfate, dermatan sulfate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/08—Bronchodilators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/08—Antiallergic agents

Definitions

  • the present invention relates to sulfated polysaccharides selected from the group of carrageenans and fucoidans and pharmaceutical compositions made thereof, wherein said sulfated polysaccharides are present as antiviral active ingredients, for medical or veterinary use in the prevention or treatment of diseases caused by or associated with a virus entering an individual's body via the respiratory tract, the virus being selected from the group of orthomyxoviridae, paramyxoviridae, adenoviridae and coronaviridae.
  • Orthomyxoviridae are RNA viruses, the most prominent members being influenza virus species. Influenza A and B virus are the two types of influenza viruses that cause epidemic human disease. They are typically spread from person to person, primarily through respiratory droplet transmission.
  • the paramyxoviridae family includes respiratory syncytial virus (RSV), parainfluenza virus and metapneumavirus.
  • RSV and parainfluenza virus infections may cause serious respiratory infections in infants and young children but may also cause severe disease in elderly persons and adults having an impaired immune system.
  • parainfluenza virus species four members are known to cause serious respiratory diseases in children for which presently no effective prevention or therapy is available, the four members being parainfluenza virus types 1 through 4.
  • Parainfluenza virus and RSV outbreaks are believed to contribute substantially to increased hospitalization and mortality rates.
  • Patients with impaired immune, cardiac, or pulmonary systems are at increased risk of running into serious complications following a paramyxovirus infection and will therefore particularly benefit from an antiviral therapy.
  • Respiratory syncytial virus which is the most wide-spread respiratory pathogen typically afflicting individuals during infancy and early childhood worldwide, causes yearly outbreaks of pneumonia and bronchiolitis during winter time and early in spring.
  • RSV bronchiolitis and pneumonia require hospitalization of hundreds of thousands of infants every year.
  • Passive protection against RSV is available at least in part at a reported 55% success rate upon monthly intramuscular injection of the humanized monoclonal anti-RSV antibody palivizumab (Synagis®).
  • coronaviridae family includes coronavirus and torovirus. Coronaviruses are known to infect the upper respiratory tract and the gastrointestinal tract in mammals and birds. It is believed that coronaviruses cause a high percentage of all common colds in human adults.
  • Adenoviridae are DNA viruses which typically infect the upper respiratory tract. At present, more than 50 different serotypes of human adenovirus are known, grouped in six subtypes A to F, which are responsible for 5-10% of upper respiratory infection in children.
  • Sulphated polysaccharides including carrageenans and fucoidan have been known for their antiviral efficacy for decades. Accordingly, the prior art is replete with scientific articles on the antiviral effects of carrageenans. In a most interesting review, Gonzalez M. E. et al. (1987, Antimicrob. Agents Chemother. 31, 1388-1393) report an antiviral efficacy of different sulphated polysaccharides including iota-carrageenan against several animal viruses.
  • Iota-carrageenan showed antiviral activity against the enveloped viruses HSV-1, HSV-2, Semliki Forest virus (SFV), vaccinia virus and African swine fever virus (ASF) and against the naked encephalomyocarditis (EMC) virus. Iota-carrageenan had no effect on the enveloped viruses vesicular stomatitis virus (VSV) and measles virus and on the naked viruses polio virus type 1 and adenovirus type 5.
  • VSV vesicular stomatitis virus
  • measles virus and on the naked viruses polio virus type 1 and adenovirus type 5.
  • U.S. Pat. No. 4,783,446 discloses an antiviral activity of iota-, kappa- and lambda-carrageenan against retroviral infection, in particular against human T-cell leukemia virus (HTLV) III infection.
  • HTLV human T-cell leukemia virus
  • WO 88/06396 discloses a method for the treatment of retroviral infections, including infection with HIV, by administering a carrageenan or a mixture of carrageenans.
  • sulphated polysaccharides like iota-, kappa-, and lambda-carrageenan have an effective inhibitory activity against the replication of hepatitis A virus (HAV).
  • HAV hepatitis A virus
  • CMV human cytomegalovirus
  • VSV vesicular stomatitis virus
  • Sindbis virus Sindbis virus
  • HIV-1 human cytomegalovirus
  • these sulphated polysaccharides were tested to be inactive against coxsackievirus, poliovirus and parainfluenza virus.
  • EP 0293826 discloses the therapeutic and prophylactic application of sulfated polysaccharides such as fucoidan and carrageenans to inhibit HIV-1 in vitro.
  • U.S. Pat. No. 5,658,893 discloses a method for inhibiting rotavirus infection of human cells by contacting the rotavirus with lambda-carrageenan. It is further disclosed that iota- and kappa-carrageenan did not exhibit anti-rotaviral activity.
  • US 2003/181415 A discloses that sulphated polysaccharides, including cellulose sulphate, are known to be effective against various enveloped viruses and in particular against herpes simplex virus (HSV), Papilloma viruses and HIV.
  • HSV herpes simplex virus
  • Papilloma viruses HIV.
  • Fucoidan is a sulphated polysaccharide mainly extracted from various species of brown seaweed.
  • HMWF high molecular weight fucoidan
  • LMWF low molecular weight fucoidan
  • F-fucoidan is mainly composed of sulphated esters of fucose, while U-fucoidan is comprised of about 20% of glucuronic acid.
  • Carrageenan is a generic term for linear sulphated galactose-based polysaccharides extracted from seaweed (rhodophyceae). It is mainly used as a thickener, gelling agent, stabilizer or emulsifier in pharmaceutical and food products.
  • Iota-carrageenan is a soft-gel forming sulphated galactan predominantly extracted from red seaweed Gigartina stellate and Chondrus crispus .
  • Kappa-carrageenan yields strong, rigid gels and is predominantly produced from Kappaphycus cottonii .
  • Lambda-carrageenan which is the most common form, is frequently used to thicken dairy products.
  • HSV-1 virions are internalized even in the presence of high concentrations of iota-carrageenan. They suggest that at least for HSV-1 carrageenan inhibits a step in virus replication subsequent to virus attachment and entry into the cell but still prior to the synthesis of late viral proteins.
  • the present invention now provides for a carrageenan- and/or fucoidan-based antiviral composition suitable in the prophylactic or therapeutic treatment of respiratory viral infections caused by members of the paramyxoviridae, orthomyxoviridae, adenoviridae and/or coronaviridae families. Contrary to the disclosure of Fujisawa H. et al. (1987, J. gen. Virol.
  • carrageenan in particular iota- and kappa-carrageenan, has indeed antiviral efficacy against various members of the orthomyxoviridae, paramyxoviridae, adenoviridae and coronaviridae.
  • fucoidan particularly the high molecular weight fucoidan (HWMF) fraction, has antiviral efficacy against various members of the orthomyxoviridae and paramyxoviridae.
  • HWMF high molecular weight fucoidan
  • the present invention aims at providing an antiviral pharmaceutical composition suitable for the prevention or treatment of respiratory tract infections caused by a virus selected from the group consisting of orthomyxovirus, paramyxovirus, adenovirus and coronavirus, as well as for diseases or pathological conditions associated with such primary viral infections, such diseases or conditions comprising secondary viral or bacterial infections as well as bodily symptoms typically associated with any such primary or secondary infection including symptoms such as fever, pain, dizziness, shivering, sweating, and/or dehydration.
  • a virus selected from the group consisting of orthomyxovirus, paramyxovirus, adenovirus and coronavirus
  • diseases or pathological conditions associated with such primary viral infections such diseases or conditions comprising secondary viral or bacterial infections as well as bodily symptoms typically associated with any such primary or secondary infection including symptoms such as fever, pain, dizziness, shivering, sweating, and/or dehydration.
  • the present invention relates to the use of carrageenan as an antiviral active ingredient in the manufacture of a pharmaceutical composition for the prophylaxis or treatment of a pathological condition or disease caused by or associated with an infection by a respiratory virus, wherein said respiratory virus comprises at least one protein attached to or integrated in its viral membrane, the protein capable of binding to a host cell via a receptor having at least one sugar moiety, the respiratory virus being selected from the group consisting of orthomyxovirus and paramyxovirus and the carrageenan being selected from the group consisting of iota- and kappa-carrageenan.
  • a “host cell” referred to herein is any eukaryotic cell that is naturally, i.e. under natural or natural-like conditions, targeted and penetrated by any of the viruses referred to hereinafter.
  • host cell lines that are typically recognized as suitable in vitro models for testing the efficiacy of physiologically active agents and which allow for at least some predictability of the results with regard to comparable human applications.
  • the present invention further relates to a pharmaceutical composition
  • a pharmaceutical composition comprising iota- and/or kappa-carrageenan as an antiviral active ingredient and to applications thereof in the prevention or treatment of diseases caused by or associated with a virus entering an individual's body via the respiratory tract, wherein said virus comprises at least one protein attached to or integrated in its viral membrane, the protein capable of binding to a host cell via a receptor having at least one sugar moiety, and the virus being selected from the group consisting of orthomyxovirus and paramyxovirus.
  • antiviral active ingredient refers to a compound that is directly or indirectly effective in specifically interfering with at least one viral action selected from the group consisting of virus penetration of eukaryotic cells, virus replication in eukaryotic cells, virus assembly, virus release from infected eukaryotic cells, or that is effective in unspecifically inhibiting a virus titer increase or in unspecifically reducing a virus titer level in a eukaryotic or mammalian host system. It also refers to a compound that prevents from or reduces the likelihood of getting a viral infection.
  • the present pharmaceutical composition may be administered, as the case may be, before or after the onset of a viral infection, i.e. for prophylactic or therapeutic treatment purposes, or for both prophylactic and therapeutic administration.
  • prophylaxis or “prophylactic treatment” as used herein relates to the administration of the present pharmaceutical composition to a healthy individual in order to reduce said individual's susceptibility for a viral infection.
  • the term “therapy” or “therapeutic treatment” as used herein relates to the administration of the present pharmaceutical composition in order to achieve a reduction in severity and/or frequency of symptoms, elimination of symptoms and/or underlying cause, prevention of the occurrence of symptoms and/or their underlying cause, and/or improvement or remediation of damage directly caused by or indirectly associated, e.g. through secondary infection, with the viral infection.
  • orthomyxo- and paramyxoviruses and several other viruses attach to the host cell via binding to receptors present on the host cell surface, the receptors typically being glycoproteins or glycolipids containing sugar residues including sialic acid (N-actyl neuramic acid) and heparan sulphate residues.
  • influenza viruses bind to sialic acid residues.
  • Human influenza viruses preferably bind to sialic acid residues having an alpha 2-6 linkage, whereas avian influenza virus preferably bind to sialic acid residues having alpha 2-3 linkage.
  • the cellular uptake of a paramyxovirus involves at least two of its integral membrane glycoproteins.
  • One of them typically selected from the group of glycoproteins HN, H and G, is involved in cell attachment and the other glycoprotein, i.e. glycoprotein F, is involved in mediating pH-independent fusion of the viral envelope with the membrane of the host cell.
  • glycoprotein F glycoprotein F
  • respiroviruses and rubulaviruses bind to sialic acid residues of glycoprotein or glycolipid host cell receptors.
  • RSV The attachment of RSV is not fully understood but attachment to the host cell most likely involves interaction with heparan sulphate, a glucoseaminoglycan that is part of the extracellular matrix.
  • this change on the host cell surface prevented orthomyxo- and paramyxoviruses from attachment to the host cells even after removal of the carrageenan from the host cells, i.e. in the absence of carrageenan at the time of artificially triggering an infection by inoculation in vitro.
  • the carrageenan compositions of the present invention may successfully be applied against orthomyxovirus infection, including infections caused by influenza A or B virus, and especially wherein said influenza A or B virus is a human virus that binds to a host cell via 2-6 sialic acid linkage.
  • Human influenza virus infections are best treated using predominantly or solely iota-carrageenan or fucoidan as the antiviral active ingredients.
  • Carrageenan may, however, also be successfully applied against infections wherein said influenza A virus is an avian virus that binds to a host cell via 2-3 sialic acid linkage.
  • said influenza A virus is an avian virus that binds to a host cell via 2-3 sialic acid linkage.
  • kappa-carrageenan turned out to be the most efficacious carrageenan, the use of which is therefore preferred in connection with avian virus prophylaxis or therapeutic treatment.
  • iota-carrageenan and fucoidan were found to be much less active or even inactive towards avian influenza A virus.
  • the paramyxovirus species found to be susceptible to carrageenan treatment are selected from the group consisting of human parainfluenza virus (HPV) type 1, HPV type 2, HPV type 3, HPV type 4 and RSV. Where the selected paramyxovirus is RSV it is preferred that iota-carrageenan or fucoidan be applied as a predominant or sole sulfated polysaccharide ingredient for prophylactic or therapeutic administration, because iota-carrgeenan and fucoidan yielded the best results in the experimental set-ups.
  • HPV human parainfluenza virus
  • the pathological conditions or diseases referred to herein in connection with prophylactic or therapeutic administration of carrageenan comprise acute bronchitis, chronic bronchitis, rhinitis, sinusitis, croup, acute bronchiolitis, pharyngitis, tonsillitis, laryngitis, tracheitis, asthma and pneumonia.
  • Carrageenan is especially suitable for topical application to treat skin or mucosal inflammation.
  • Carrageenan useful for topical application to the skin or mucosa in accordance with the present invention has a molecular weight ranging from about 15,000 to 5,000,000 Da, fractions having average molecular weights of more than 50,000 Da, and especially fractions having average molecular weights in the range of from 50,000 to 3,000,000 Da being particularly preferred.
  • Systemic e.g. parenteral or oral administration is possible, too, especially using lower molecular weight polymers having an average molecular weight in the 15,000 to 100,000 Da range.
  • the pharmaceutical composition according to the present invention adjusted for parenteral use may be provided as a preparation selected from the group of skin lotions, creams, ointments, gels, powders including powders for inhalation, sprays, foams, liquid drops or gargle solutions.
  • the carrageenan preparations may, however also be adjusted for oral administration, e.g. as liquid solutions, or semi-solid or solid preparations such as dry powders, tablets, capsules, dragees or any other orally ingestible galenic form.
  • Pharmaceutical compositions for mucosal application include nose sprays or drops and any other nasal drug delivery system known in the art such as disclosed, for example, in U.S. Pat. No. 6,391,452.
  • composition typically for topical use and is liquid or semi-solid it typically comprises in its ready-for-use form carrageenan in an amount of between 0.01 and 10%, preferably between 0.01 and 5%, most preferably between 0.1 and 2% by weight, relative to the total volume of the composition.
  • composition is solid it typically comprises in its ready-for-use form carrageenan in an amount of between 0.01 and 10%, preferably between 0.01 and 5%, most preferably between 0.1 and 2% by weight relative to the total weight of the composition.
  • the carrageenans useful in the present invention are commercially available but may also be prepared by extraction from seaweed plants pursuant to extraction procedures known in the art.
  • carboxystyrene refers to either iota-, or kappa-carrageenan or a mixture of both.
  • the carrageenan of the present invention can further be a homopolymer or a heteropolymer.
  • a carrageenan homopolymer is built of subunits of only one kind of either iota-, or kappa-carrageenan, whereas a carrageenan heteropolymer comprises subunits of both said carrageenans.
  • a “mixture” of carrageenans may thus also refer to a composition of matter comprising a mixture of different carrageenan subunits as part of at least one heteropolymeric carrageenan present in said composition.
  • the antiviral pharmaceutical compositions according to the present invention are substantially free of carrageenans other than iota- and kappa-carrageenan, i.e. comprise either iota- or kappa-carrageenan, or a mixture of both, in an amount of 80% or more, preferably of 90% or more, and especially of up to 99% (w/w) or more, relative to the dry weight of all carrageenans present in the composition.
  • the composition is substantially comprised solely of iota-carrageen, while for other applications the composition may comprise more than 50%, preferably more than 70%, and especially up to more than 95% (w/w) by dry weight of iota-carrageenan, relative to the total dry weight of all carrageenans present in the composition.
  • the composition comprises more than 50%, preferably more than 70%, and especially up to more than 95% (w/w) by dry weight of kappa-carrageenan, relative to the total dry weight of all carrageenans present in the composition.
  • composition comprises at least one heteropolymeric carrageenan
  • numeric values given above relate to the percentages of the respective carrageenan subunits.
  • the antiviral carrageenan compositions in accordance with the present invention may further comprise at least one pharmaceutically acceptable carrier and/or additive suitable and permitted for medical application.
  • the carrier may be a diluent, e.g. water, saline, optionally phosphate buffered saline (PBS), an excipient, or another vehicle which facilitates the administration of the composition.
  • the groups of carriers and additives, respectively may comprise but are not limited to SiO 2 , TiO 2 , a binder such as microcrystalline cellulose, polyvinylpyrrolidone, gum tragacanth, gelatine, starch, lactose, lactose monohydrate, alginic acid or maize starch; a lubricant or surfactant like magnesium stearate or sodium lauryl sulphate; a glidant like e.g. colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin.
  • a binder such as microcrystalline cellulose, polyvinylpyrrolidone, gum tragacanth, gelatine, starch, lactose, lactose monohydrate, alginic
  • Still further additives may be selected from the group consisting of physiologically acceptable preservatives, pharmaceutically acceptable alkali metal salts like sodium, potassium, lithium or ammonium chlorides, buffers or pH adjusting agents such as citric acid, acetic acid, fumaric acid, hydrochloric acid, malic acid, nitric acid, phosphoric acid, propionic acid, sulphuric acid and tartaric acid, and combinations of said acids.
  • physiologically acceptable preservatives such as citric acid, acetic acid, fumaric acid, hydrochloric acid, malic acid, nitric acid, phosphoric acid, propionic acid, sulphuric acid and tartaric acid, and combinations of said acids.
  • composition comprises sodium chloride as an additive.
  • a pharmaceutically acceptable salt is present in the composition in an amount of not more than 1%, preferably not more than 0.6% (w/v).
  • the carrageenan itself may be present as a salt, too, preferably as a sodium salt.
  • compositions for prophylactic or therapeutic medical application be provided as sterile, germ-free, pathogen-free, pyrogen-free and/or allergen-free preparations.
  • Carrageenan was found to be non-toxic upon oral or dermal administration, or upon inhalation, even when applied at extremely high doses. It was therefore classified as “generally recognized as safe” (GRAS) by the Food and Drug Administration (FDA).
  • GRAS generally recognized as safe
  • Carrageenan especially iota-carrageenan, can also be used as an antiviral agent effective against orthomyxovirus and paramyxovirus infections in various medicinal products together with other physiologically active compounds or drugs as the main active ingredients, wherein the role of carrageenan includes its use as a carrier or as an additive such as an emulsifier or a viscosity modifying agent. It is compatible with most pharmaceutical preparations without causing undesired side-effects.
  • compositions comprising carrageenan as an additive, more specifically as an antiviral, particularly anti-paramyxoviral or anti-orthomyxoviral, adjuvant to medicaments that are typically applied in the prophylaxis or therapy of infectious and/or inflammatory diseases, allergies and/or conditions of an impaired or suppressed immune system.
  • carrageenan preferably iota-carrageenan, as an anti-influenza virus effective adjuvant in combination with medicaments useful in the prophylaxis or therapy of infectious and/or inflammatory diseases, allergies and/or conditions of an impaired or suppressed immune system.
  • carrageenan may also have immuno-modulating activity, particularly immune system strengthening activity. It is not yet clear, however, whether this activity is directly associated with a physiological interference of the substance or indirectly through its antiviral efficacy.
  • composition of the present invention will usually be formulated into preparations for topical or mucosal use, preferably selected from sprays, particularly nose sprays, powders, drops, gargle solutions, foams, gels, creams, ointments, lotions, lozenges, and the like.
  • the pharmaceutical composition of the present invention may also be coated onto solid surfaces of hygiene or sanitary items such as, for example, facial hygiene or sanitation articles that are typically used in the oral and/or nasal areas including but not limited to nasal tissues or papers, and handkerchiefs.
  • the pharmaceutical composition may be applied, e.g. sprayed—much like disinfectants—onto gloves, hygiene tissues or papers including nasal tissues or papers, in order to exert a virucide effect at least to some extent, thus contributing to reducing an individual's repeated self-infection by contaminated fingertips and also to reduce viral distribution among different individuals that are in close, e.g. hand-to-hand, contact with each other.
  • carrageenan may also be used as an antiviral active ingredient in the manufacture of a pharmaceutical composition for the prophylaxis or therapeutic treatment of a pathological condition or disease caused by or associated with an infection by a respiratory virus being selected from the group consisting of adenovirus and coronavirus, the adenovirus preferably being adenovirus type B (Ad50).
  • Adenovirus and coronavirus infections are best treated using compositions where iota-carrageenan is the sole or predominant active antiviral ingredient.
  • fucoidan is usefully applied as an antiviral active ingredient in the manufacture of a pharmaceutical composition effective for the prophylaxis or treatment of pathological conditions or diseases caused by or associated with a respiratory virus infection, the respiratory virus selected from the group consisting of orthomyxo- and paramyxoviruses.
  • the orthmyxovirus is typically influenza virus A or B
  • the paramyxovirus is typically RSV.
  • a fucoidan-based composition in its ready-for-use form for topical or oral administration may comprise fucoidan in an amount of between 0.01 and 10%, preferably between 0.01 and 5%, most preferably between 0.1 and 2% weight per volume (w/v) or weight per weight (w/w) depending on whether the composition is liquid or semi-solid (w/v-percentages) or solid (w/w-percentages). It may further comprise at least one pharmaceutically acceptable carrier and/or additive, as well as other physiologically active substances or drugs. For example, sodium chloride is frequently used as an additive. In general, pharmaceutically acceptable salts may be present in the fucoidan compositions in an amount of not more than 1%, preferably not more than 0.6%.
  • FIG. 1 shows the efficacy of iota-carrageenan in reducing plaque formation of influenza A/Chile/1/93H1N1 virus in MDCK cells at different doses ranging from a final concentration of 0.1 to 100 ⁇ g/ml.
  • FIG. 2 shows the efficacy of iota-carrageenan in reducing plaque formation of influenza A/Aichi2/68H3N2 virus in MDCK cells at different doses ranging from a final concentration of 75 to 300 ⁇ g/ml.
  • FIG. 3 shows the efficacy of iota-carrageenan in reducing plaque formation of parainfluenza virus 3 in Hep-2 cells at different doses ranging from a final concentration of 0.1 to 100 ⁇ g/ml.
  • FIG. 4 shows the efficacy of iota-carrageenan pretreatment in reducing plaque formation of parainfluenza virus 3 in HeLa cells at different doses ranging from a final concentration of 13 to 400 ⁇ g/ml.
  • FIG. 5 shows the results of a coronavirus induced cell death inhibition experiment in cat kidney (CK) cells.
  • FIG. 6 shows the results of a HRV8 induced cell death inhibition experiment in HeLa cells.
  • FIG. 7 shows the binding of FITC-labelled iota-carrageenan to HeLa cells after successive washing with PBS.
  • FIG. 8 shows the results of a RSV induced cell death inhibition experiment in Vero cells.
  • FIG. 9 shows the results of an RSV induced cell death inhibition experiment in HNep cells.
  • FIG. 10 shows the results of an RSV induced cell death inhibition experiment in HNep cells.
  • FIG. 11 shows the results of a RSV induced cell death inhibition experiment in HEp-2 cells.
  • FIG. 12 shows the results of a RSV induced cell death inhibition experiment in HNep cells.
  • FIG. 13 shows the results of a parainfluenza virus 3 induced cell death inhibition experiment in HNep cells.
  • FIG. 14 shows the results of an influenza virus H3N2 (FLU) induced cell death inhibition experiment in HNep cells.
  • FIG. 15 shows the efficacy of iota-, kappa-, lambda-carrageenan and fucoidan in reducing plaque formation of influenza A H5N1 virus in MDCK cells at doses of 400 ⁇ g/ml and 44 ⁇ g/ml.
  • FIG. 16 shows the results of an adenovirus type 50 (Ad50) induced cell death inhibition experiment in HNep cells.
  • FIG. 17 shows the results of an Ad50 induced cell death inhibition experiment in HNep cells.
  • Virus suspensions containing 60-80 pfu of influenza virus A/Chile/1/93 H1N1 were mixed with a iota-carrageenan stock solution to final concentrations of 0.1, 1, 10, 25, 50 or 100 ⁇ g/ml.
  • Confluent monolayers of the canine kidney cell line MDCK in six well plates were infected with the virus suspensions for 60 min at 34° C. The infection inoculum was removed and cells were washed with PBS and agarose overlay containing 0.6% agarose was added. Plates were incubated at 36° C. in a humidified atmosphere of 5% CO 2 in air. 48-60 h after infection the agarose overlay was removed, cells were stained with crystal violet stain and visible plaques were counted. The percentage of plaque formation relative to the infected control (without iota-carrageenan treatment) was determined for each iota-carrageenan concentration.
  • iota-carrageenan inhibits, in a dose dependent manner, the plaque formation of influenza A/Chile/1/93H1N1 virus in MDCK cells.
  • a 50% reduction in plaque number (IC50) was achieved at a iota-carrageenan concentration of 50 ⁇ g/ml.
  • Virus suspensions containing 60-80 pfu of influenza virus A/Aichi2/68H3N2 were mixed with a iota-carrageenan stock solution to final concentrations of 75, 150 or 300 ⁇ g/ml.
  • Confluent monolayers of the canine kidney cell line MDCK in six well plates were infected with the virus suspensions for 60 min at 34° C. The infection inoculum was removed and cells were washed with PBS and agarose overlay containing 0.6% agarose was added. Plates were incubated at 37° C. in a humidified atmosphere of 5% CO 2 in air. 48-60 h after infection the agarose overlay was removed, cells were stained with crystal violet stain and visible plaques were counted. The percentage of plaque formation relative to the infected control (without iota-carrageenan) was determined for each iota-carrageenan concentration.
  • iota-carrageenan inhibits, in a dose dependent manner, the plaque formation of influenza A/Aichi/2/68H3N2 virus in MDCK cells.
  • Virus suspensions containing 60-80 pfu of parainfluenza virus 3 were mixed with a iota-carrageenan stock solution to final concentrations of 0.1, 1, 10, 25, 50 and 100 ⁇ g/ml. The mixture was incubated for 1 h at 34° C. Confluent monolayers of Hep-2 cells in six well plates were infected with the virus suspensions for 60 minutes a 34° C. The infection inoculum was removed and cells were washed with PBS and agarose overlay containing 0.6% agarose was added. The trays were incubated in a humidified, 5% CO 2 atmosphere.
  • iota-carrageenan inhibits, in a dose dependent manner, the plaque formation of parainfluenza virus 3 in Hep-2 cells.
  • a 50% reduction in plaque number (IC50) was achieved at an iota-carrageenan concentration of 10 ⁇ g/ml.
  • iota-carrageenan inhibits the plaque formation of parainfluenza virus 3 at a concentration of 400 and 133 ⁇ g/ml when cells were preincubated for three hours with iota-carrageenan, although no iota-carrageenan was present at the time of the infection and during the entire subsequent incubation period at 37° C.
  • This result indicates that iota-carrageenan chemically or structurally modifies the surface receptors of the host cell in a way such that receptor-mediated binding of parainfluenza virus 3 to the host cell is hindered or prevented even in the absence of the modifying agent carrageenan. This is also proof of a strong prophylactic efficacy of iota-carrageenan.
  • Subconfluent CK cells were infected in the presence of iota-, kappa- or lambda carrageenan at a concentration of 4, 40 and 400 ⁇ g/ml with feline coronavirus FIPV at a moi (multiplicity of infection) of 0.1) (see FIG. 5A ).
  • subconfluent CK cells were incubated three hours with iota-, lambda or kappa-carrageenan at a concentration of 4, 40, and 400 ⁇ g/ml.
  • the percentage of viable cells relative to the uninfected control was determined for each carrageenan and each concentration.
  • HRV8 human rhinovirus type 8
  • subconfluent HeLa cells were incubated for three hours with iota-carrageenan at a concentration of 4, 40 and 400 ⁇ g/ml prior to infection.
  • the supernatant containing iota-carrageenan was removed and the cells were washed three times with PBS and then infected with HRV8 in the absence of the polymer (see FIG. 6B ).
  • the percentage of viable cells relative to the uninfected control was determined for each iota-carrageenan concentration.
  • iota-carrageenan inhibits the HRV8 mediated cell death at all concentrations.
  • FIG. 6B it can be taken that, in contrast to viruses that enter the cell via sugar receptors, HRV8 mediated cell death was not inhibited by more than 5% even at 400 ⁇ g/ml and where cells were preincubated for three hours with iota-carrageenan.
  • This result is consistent with previous findings (data not shown) and suggests that for HRV8 the preincubation of the target cells with carrageenan does not significantly protect the target cells from becoming infected if carrageenan is missing at the time of infection.
  • This result indicates that the receptor for HRV8, the LDL-receptor, is not modified or masked by the treatment with iota-carrageenan.
  • Iota-Carrageenan is Quantitatively Removed from HeLa Cells by Washing the Cells with PBS
  • FITC-labelled iota-carrageenan at a concentration of 400, 133, 4 and 0.4 ⁇ g/ml for 10 minutes, then the supernatant was removed and the cells were washed three times with PBS.
  • the amount of residual FITC-labelled carrageenan was determined with a fluorescent detection reader (BMG-Omega) after removing the supernatant containing FITC-labelled iota-carrageenan and after each washing step.
  • the fluorescent light units were converted into concentration values of ⁇ g/ml carrageenan by using a standard curve.
  • FITC-labelled iota-carrageenan was quantitatively removed (>95%) from HeLa cells by washing the cells at least three times with PBS. This result proves that carrageenan does not covalently bind to the surface of the cells. The result was confirmed by a second experimental set, where the incubation period was extended from 10 minutes to 3 hours using the same detection method. The results thus support the conclusions drawn herein and in the examples concerned that carrageenan induces a modification of the sugar receptors on the target cell surface involved in the binding of the virions, which modification is still present after removal of the polymer from the target cell surface.
  • iota-carrageenan inhibits the RSV mediated cell death at all concentrations even if the polymer is absent at the time of and during infection. It is known that RSV attaches to the cells via heparan sulphate, a sugar molecule that is present on the surface of epithelial cells. The results indicate that this receptor is modified by iota-carrageenan and the attachment of RSV to the cell surface and the subsequent viral replication is thereby blocked.
  • iota-carrageenan significantly inhibits the RSV mediated cell death at a concentration of 100 ⁇ g/ml even when the polymer was added only 24 hours after infection. This result demonstrates that iota-carrageenan cannot only be used prophylactically but may also exert antiviral efficacy in the course of a therapeutic treatment when applied in the early phase of viral infection.
  • iota-carrageenan significantly inhibits the RSV mediated cell death even at a concentration as low as 1 ⁇ g/ml when the polymer was present during infection. This result demonstrates that iota-carrageenan can be used effectively for prophylactic intervention strategies.
  • fucoidan significantly inhibits the RSV mediated cell death in HNep cells at a concentration of 0.4 ⁇ g/ml when the polymer is present during infection. Fucoidan is therefore an interesting candidate for the development of products for prophylaxis and treatment of RSV infections.
  • Subconfluent HNep cells were infected in the presence of either iota-, kappa-, lambda-carrageenan or fucoidan at a concentration of 400, 133.3, 44.4, 14.8, 4, 1.3 and 0.4 ⁇ g/ml with parainfluenza virus type 3.
  • the percentage of viable (hence uninfected) HNep cells relative to the uninfected control was determined for each polymer and each polymer concentration.
  • Subconfluent HNep cells were infected in the presence of iota-, kappa-, lambda-carrageenan and fucoidan at a concentration of 400, 133.3, 44.4, 14.8, 4, 1.3 and 0.4 ⁇ g/ml with influenza virus H3N2.
  • the percentage of viable HNep cells relative to the uninfected control was determined for each polymer and each polymer concentration.
  • Virus suspensions containing 60-80 pfu of avian influenza virus H5N1 were mixed with a polymer stock solution of either iota-, kappa-, lambda-carrageenan or fucoidan to final polymer concentrations of 400 or 44.4 ⁇ g/ml.
  • Confluent monolayers of the canine kidney cell line MDCK in six well plates were infected with the virus suspensions for 60 min at 34° C. The infection inoculum was removed and cells were washed with PBS and agarose overlay containing 0.6% agarose was added. Plates were incubated at 36° C. in a humidified atmosphere of 5% CO 2 in air. 48-60 h after infection the agarose overlay was removed, cells were stained with crystal violet stain and visible plaques were counted. The percentage of plaque formation relative to the infected control (without polymer treatment) was determined for each polymer and each polymer concentration.
  • avian influenza virus H5N1 As shown in FIG. 15 , it was found that plaque formation of avian influenza virus H5N1 was not influenced by iota- and lambda carrageenan and fucoidan. However, kappa-carrageenan inhibits, in a dose dependent manner, the plaque formation of avian influenza virus H5N1 in MDCK cells. Since avian influenza virus preferably binds to sialic acid residues with alpha 2-3 linkage the results indicate that kappa-carrageenan may preferably modify such sialic acid residues having 2-3 linkage.
  • Subconfluent HNep cells were infected in the presence of iota-carrageenan at a concentration of 400, 133.3, 44.4, 14.8, 4, 1.3 and 0.4 ⁇ g/ml with Ad50. The percentage of viable HNep cells relative to the uninfected control was determined for each iota-carrageenan concentration.
  • iota-carrageenan significantly inhibits the Ad50 mediated cell death even at a concentration as low as 4 ⁇ g/ml when the polymer is present at the time of and during infection. This result is an indication that iota-carrageenan may be used for effective prophylactic intervention strategies against adenovirus of the subtype B (e.g. Ad50).
  • adenovirus of the subtype B e.g. Ad50
  • Subconfluent HNep cells were infected in the presence of iota-carrageenan at a concentration of 400, 40 and 4 ⁇ g/ml with Ad50.
  • subconfluent HNep cells were incubated for three hours with iota-carrageenan at a concentration of 400, 40 and 4 ⁇ g/ml prior to infection.
  • the supernatant containing iota-carrageenan was removed and the cells were washed three times with PBS and infected with Ad50 in the absence of the carrageenan.
  • the percentage of viable HNep cells relative to the uninfected control was determined for each iota-carrageenan concentration.
  • iota-carrageenan significantly inhibits the Ad50 mediated cell death at a concentration of 400 and 40 ⁇ g/ml in the presence and in the absence of iota-carrageenan at the time of infection and during the observed infection period.

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013006795A2 (en) 2011-07-07 2013-01-10 Humanitas International Foundation Antiviral compositions and methods of their use
US20160296616A1 (en) * 2013-01-25 2016-10-13 Board Of Regents, The University Of Texas System Immunogenic compositions and uses thereof
US20170196783A1 (en) * 2016-01-13 2017-07-13 Jehangir Gowani Pharyngeal or buccal cavity rinse and process of use thereof
US10220055B2 (en) 2014-01-22 2019-03-05 Nicox S.A. Composition effective against viral conjunctivitis
WO2019046426A1 (en) * 2017-08-30 2019-03-07 Applied Biological Laboratories, Inc. COMPOSITIONS AND METHODS FOR PROTECTING PATHOGENS AND IRRITANTS IN AIR SUSPENSION
US10646438B2 (en) 2010-07-26 2020-05-12 Board Of Regents, The University Of Texas System Methods for inducing an immune response via buccal and/or sublingual administration of a vaccine
US10660914B2 (en) * 2015-07-14 2020-05-26 Marinomed Biotech Ag Stuffy nose deblocking composition having antiviral activity
WO2021168173A1 (en) * 2020-02-20 2021-08-26 Synkine Therapeutics, Inc. Methods and compositions for treating viral respiratory infections
WO2021202952A1 (en) * 2020-04-02 2021-10-07 Linhardt Robert John Compositions incorporating sulfated polysaccharides for inhibiting sars-cov-2
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9884024B2 (en) * 2005-12-27 2018-02-06 Hemant N Joshi Physically dispersed, molecularly dissolved and/or chemically bound drug(s) in an empty, hard capsule shell composition
JP5687619B2 (ja) * 2008-07-01 2015-03-18 マリノメド バイオテクノロジー ゲゼルシャフト ミット ベシュレンクテル ハフツング 抗アレルギー性の海洋性バイオポリマー
WO2010086746A2 (en) * 2009-01-28 2010-08-05 Life Science Nutrition As Compositions and methods of treating viral infections
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ES2531367T3 (es) * 2009-12-22 2015-03-13 Marinomed Biotechnologie Gmbh Composición antivírica sinérgica y uso de la misma
JP2011168533A (ja) * 2010-02-18 2011-09-01 Kyosei Seiyaku Kk 抗インフルエンザウイルス剤
US10786528B2 (en) * 2010-02-22 2020-09-29 Helen Fitton Anti-viral formulations
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WO2012126777A1 (en) * 2011-03-18 2012-09-27 Marinomed Biotechnologie Gmbh Inhibition of neuraminidase activity
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US11045519B2 (en) 2017-01-31 2021-06-29 Unm Rainforest Innovations Arginine-rich polypeptide compositions and methods of using same
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US20230140869A1 (en) * 2018-07-27 2023-05-11 ARC Medical Inc. High-molecular-weight fucans for treating fibrous adhesions and other diseases and conditions
KR101995376B1 (ko) * 2018-08-20 2019-07-02 한미약품 주식회사 카라기난을 유효성분으로 포함하는 항-바이러스 조성물
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US11821111B2 (en) 2019-11-15 2023-11-21 Fred Hutchinson Cancer Center Barcoded influenza viruses and deep mutational scanning libraries including the same
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GB202109888D0 (en) 2021-07-08 2021-08-25 Pollinner Ltd Nasal spray
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CN114395538A (zh) * 2022-01-19 2022-04-26 和元生物技术（上海）股份有限公司 一种促进重组病毒载体向细胞外分泌的方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4783446A (en) * 1985-11-22 1988-11-08 Neushul Mariculture Incorporated Method for the treatment of AIDS virus and other retroviruses
WO1994015624A1 (en) * 1993-01-08 1994-07-21 The Population Council Use of sulphated polysaccharides for preventing sexually transmitted diseases
US5658893A (en) * 1995-03-29 1997-08-19 Abbott Laboratories Method for inhibition of rotavirus infection with carrageenan
US6391452B1 (en) * 1997-07-18 2002-05-21 Bayer Corporation Compositions for nasal drug delivery, methods of making same, and methods of removing residual solvent from pharmaceutical preparations
US6623727B2 (en) * 2000-10-20 2003-09-23 Wella Aktiengesellschaft Solid, form-stable gels for hair treatment
US20030181416A1 (en) * 2002-01-10 2003-09-25 Comper Wayne D. Antimicrobial charged polymers that exhibit resistance to lysosomal degradation during kidney filtration and renal passage, compositions and method of use thereof
US20050261240A1 (en) * 2002-04-30 2005-11-24 The Population Council, Inc. Carrageenan based antimicrobial compositions
US20080241271A1 (en) * 2006-11-08 2008-10-02 Roman Stephen B Foaming anti-adhesion composition

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293826A3 (en) 1987-06-02 1989-05-10 Stichting REGA V.Z.W. Therapeutic and prophylactic application of sulfated polysaccharides against aids
CH680265A5 (en) * 1990-06-25 1992-07-31 Madeleine Luescher Mattli Dr Polyanionic cpds. for treatment of myxo- and para-myxo-virus diseases - effective against e.g. influenza, measles, mumps and distemper and use e.g. a sulphonated polysaccharide
AU1052492A (en) * 1991-01-31 1992-08-06 Farmitalia Carlo Erba S.R.L. Synergistic composition comprising a fibroblast growth factor and a sulfated polylsaccharide, for use as antiviral agent
DE69717147T2 (de) 1996-08-12 2003-07-24 Merck & Co Inc Thrombin inhibitoren
JPH1180003A (ja) * 1997-09-16 1999-03-23 Yakult Honsha Co Ltd 感染症予防・治療剤
DE19961307A1 (de) 1999-12-18 2001-07-12 Krewel Meuselbach Gmbh Medizinprodukt zur Befeuchtung und Reinigung der Nasenschleimhaut
JP2001181188A (ja) * 1999-12-28 2001-07-03 Marine Biotechnol Inst Co Ltd インフルエンザウイルス感染阻害剤
FR2808687B1 (fr) 2000-04-27 2003-12-05 Goemar Lab Sa Medicament contenant des substances polysaccharidiques pour l'activation de l'apoptose
US7078392B2 (en) 2000-06-30 2006-07-18 Polydex Pharmaceuticals Limited Cellulose sulfate and other sulfated polysaccharides to prevent and treat papilloma virus infection and other infections
JP2003081841A (ja) 2001-06-26 2003-03-19 Kikkoman Corp 抗アレルギー体質強化剤
CA2512434C (en) * 2003-02-26 2012-01-03 Otsuka Pharmaceutical Co., Ltd. Sustained-release pharmaceutical composition comprising carrageenan for lung administration
US20050009782A1 (en) 2003-07-09 2005-01-13 Comper Wayne D. Antiviral charged polymers that exhibit resistance to lysosomal degradation during kidney filtration and renal passage, compositions and methods of use thereof
US20080004236A1 (en) 2004-02-06 2008-01-03 Comper Wayne D High Dose, Short Interval Use of Sulfated Polysaccharides for Treatment of Infections
US20080184618A1 (en) * 2005-08-03 2008-08-07 Amcol International Virus-Interacting Layered Phyllosilicates and Methods of Use

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4783446A (en) * 1985-11-22 1988-11-08 Neushul Mariculture Incorporated Method for the treatment of AIDS virus and other retroviruses
WO1994015624A1 (en) * 1993-01-08 1994-07-21 The Population Council Use of sulphated polysaccharides for preventing sexually transmitted diseases
US5658893A (en) * 1995-03-29 1997-08-19 Abbott Laboratories Method for inhibition of rotavirus infection with carrageenan
US6391452B1 (en) * 1997-07-18 2002-05-21 Bayer Corporation Compositions for nasal drug delivery, methods of making same, and methods of removing residual solvent from pharmaceutical preparations
US6623727B2 (en) * 2000-10-20 2003-09-23 Wella Aktiengesellschaft Solid, form-stable gels for hair treatment
US20030181416A1 (en) * 2002-01-10 2003-09-25 Comper Wayne D. Antimicrobial charged polymers that exhibit resistance to lysosomal degradation during kidney filtration and renal passage, compositions and method of use thereof
US20050261240A1 (en) * 2002-04-30 2005-11-24 The Population Council, Inc. Carrageenan based antimicrobial compositions
US20080241271A1 (en) * 2006-11-08 2008-10-02 Roman Stephen B Foaming anti-adhesion composition

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
Centers for Disease Control and Prevention, "Vaccine Effectiveness - How Well Does the Flu Vaccine Work?", two pages; page last updated 13 October 2011; can be accessed at http://www.cdc.gov/flu/about/qa/vaccineeffect.htm *
Food and Drug Administration, U.S. Department of Health and Human Services, "Guidance for Industry: Container Closure Systems for Packaging Human Drugs and Biologics", May 1999, total 56 pages *
Tsintsadze, Ted, Annals of Biomedical Research and Education, "Application of Antiviral Preparation "Super Lysine Plus" in a Complex of Treatment of Chronic Recurrent Herpetic Stomatitis", April/June 2004, vol. 4, issue 2, pp.92-93 *
Turner, Ronald, Merck Manual Professional, "Respiratory Viruses: Adenovirus Infection", total two pages; last review/revision October 2009; also can be accessed at http://www.merckmanuals.com/professional/print/infectious_diseases/respiratory_viruses/adenovirus_infection.html *
Turner, Ronald, Merck Manual Professionals, "Respiratory Viruses: Common Cold", two total pages; last review/revision October 2009; also can be accessed at http://www.merckmanuals.com/professional/print/infectious_diseases/respiratory_viruses/common_cold.html *
WordNet Search 3.0, "prevent", also can be accessed at http://wordnetweb.princeton.edu/perl/webwn?s=prevent&o2=&o0=1&o8=1&o1=1&o7=&o5=&o9=&o6=&o3=&o4=&h=; last viewed 10 March 2012 *

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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WO2013006795A2 (en) 2011-07-07 2013-01-10 Humanitas International Foundation Antiviral compositions and methods of their use
US20160296616A1 (en) * 2013-01-25 2016-10-13 Board Of Regents, The University Of Texas System Immunogenic compositions and uses thereof
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US10660914B2 (en) * 2015-07-14 2020-05-26 Marinomed Biotech Ag Stuffy nose deblocking composition having antiviral activity
US10434119B2 (en) * 2016-01-13 2019-10-08 Gargle Water, Inc. Pharyngeal or buccal cavity rinse and process of use thereof
US20170196783A1 (en) * 2016-01-13 2017-07-13 Jehangir Gowani Pharyngeal or buccal cavity rinse and process of use thereof
WO2019046426A1 (en) * 2017-08-30 2019-03-07 Applied Biological Laboratories, Inc. COMPOSITIONS AND METHODS FOR PROTECTING PATHOGENS AND IRRITANTS IN AIR SUSPENSION
WO2021168173A1 (en) * 2020-02-20 2021-08-26 Synkine Therapeutics, Inc. Methods and compositions for treating viral respiratory infections
WO2021202952A1 (en) * 2020-04-02 2021-10-07 Linhardt Robert John Compositions incorporating sulfated polysaccharides for inhibiting sars-cov-2
CN114264530A (zh) * 2021-12-30 2022-04-01 重庆市畜牧科学院 一种基于Avicel的病毒蚀斑测定方法及其应用

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