UA40598C2 - Спосіб одержання трансгенної рослини, послідовність днк, яка кодує поліфруктаh (леван) сахаразу, рослинна клітина - Google Patents

Спосіб одержання трансгенної рослини, послідовність днк, яка кодує поліфруктаh (леван) сахаразу, рослинна клітина

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Publication number
UA40598C2
UA40598C2 UA95028136A UA95028136A UA40598C2 UA 40598 C2 UA40598 C2 UA 40598C2 UA 95028136 A UA95028136 A UA 95028136A UA 95028136 A UA95028136 A UA 95028136A UA 40598 C2 UA40598 C2 UA 40598C2
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UA
Ukraine
Prior art keywords
polyfructan
cell
obtaining transgenic
plant
sequence coding
Prior art date
Application number
UA95028136A
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English (en)
Russian (ru)
Inventor
Мануела Рьобер
Мануэла Ребер
Гебхардт Гайєр
Гебхардт Гайер
Клаус Гайдер
Лотар Вілльмітцер
Лотар Вилльмитцер
Original Assignee
Хьохст Шерінг Агрево Гмбх
Хьохст Шеринг Агрево Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Хьохст Шерінг Агрево Гмбх, Хьохст Шеринг Агрево Гмбх filed Critical Хьохст Шерінг Агрево Гмбх
Publication of UA40598C2 publication Critical patent/UA40598C2/uk

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1055—Levansucrase (2.4.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Ввинахід належить до послідовностей ДНК, що ведуть до утворення поліфруктанів (леванів), а також способу одержання трансгенних рослин за допомогою плазмід, в яких знаходяться ці послідовності ДНК. Спосіб одержання трансгенної рослини, що характеризується модифікованим утворенням поліфруктану в хлоропластах, амілопластах, мітохондріях, цитозолі та/або вакуолях, який включає наступні стадії: а) введення виділеної молекули ДНК в рослинну клітину таким чином, що вона здатна вбудовуватись в геном вказаної клітину, причому вказана молекула ДНК включає (I) промотор, здатний регулювати транскрипцію гена левансахарази у вказаній клітину; (II) послідовність ДНК, що кодує поліфруктан (леван) сахаразу; та (III) 3'-нетрансльована послідовність, що здатна функціонувати як послідовнoсті термінації транскрипції; та б) відновлення інтактної цілої рослини вказаної рослинної клітину.
UA95028136A 1992-08-12 1993-08-09 Спосіб одержання трансгенної рослини, послідовність днк, яка кодує поліфруктаh (леван) сахаразу, рослинна клітина UA40598C2 (uk)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4227061A DE4227061A1 (de) 1992-08-12 1992-08-12 DNA-Sequenzen, die in der Pflanze die Bildung von Polyfructanen (Lävanen) hervorrufen, Plasmide enthaltend diese Sequenzen sowie Verfahren zur Herstellung transgener Pflanzen
PCT/EP1993/002110 WO1994004692A1 (en) 1992-08-12 1993-08-09 Dna sequences which lead to the formation of polyfructans (levans), plasmids containing these sequences as well as a process for preparing transgenic plants

Publications (1)

Publication Number Publication Date
UA40598C2 true UA40598C2 (uk) 2001-08-15

Family

ID=6465641

Family Applications (1)

Application Number Title Priority Date Filing Date
UA95028136A UA40598C2 (uk) 1992-08-12 1993-08-09 Спосіб одержання трансгенної рослини, послідовність днк, яка кодує поліфруктаh (леван) сахаразу, рослинна клітина

Country Status (12)

Country Link
US (3) US5792923A (uk)
EP (1) EP0663956B1 (uk)
JP (1) JP3643591B2 (uk)
KR (1) KR950703059A (uk)
AT (1) ATE297998T1 (uk)
AU (1) AU682472B2 (uk)
CA (1) CA2142308C (uk)
DE (2) DE4227061A1 (uk)
HU (1) HUT70977A (uk)
IL (1) IL106646A0 (uk)
UA (1) UA40598C2 (uk)
WO (1) WO1994004692A1 (uk)

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* Cited by examiner, † Cited by third party
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DE4227061A1 (de) 1994-02-17
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