CA2372632A1 - Plant acyltransferases - Google Patents
Plant acyltransferases Download PDFInfo
- Publication number
- CA2372632A1 CA2372632A1 CA002372632A CA2372632A CA2372632A1 CA 2372632 A1 CA2372632 A1 CA 2372632A1 CA 002372632 A CA002372632 A CA 002372632A CA 2372632 A CA2372632 A CA 2372632A CA 2372632 A1 CA2372632 A1 CA 2372632A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- seq
- nucleotide sequence
- plant
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A gene encoding diacylglycerol acyltransferase from Brassica napus is described, as well as methods of altering oil content in plants using the diacylglycerol acyltransferase.
Claims (21)
1. An isolated nucleic acid having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:3, or to a fragment of the nucleotide sequence of SEQ ID NO:3, said fragment being at least 15 nucleotides in length.
2. The nucleic acid of claim 1, said nucleic acid having at least 85%
sequence identity to the nucleotide sequence of SEQ ID NO:3 or to said fragment.
sequence identity to the nucleotide sequence of SEQ ID NO:3 or to said fragment.
3. The nucleic acid of claim 1, said nucleic acid having at least 90%
sequence identity to the nucleotide sequence of SEQ ID NO:3 or to said fragment.
sequence identity to the nucleotide sequence of SEQ ID NO:3 or to said fragment.
4. The nucleic acid of claim 1, said nucleic acid having at least 95%
sequence identity to the nucleotide sequence of SEQ ID NO:3 or to said fragment.
sequence identity to the nucleotide sequence of SEQ ID NO:3 or to said fragment.
5. An isolated nucleic acid having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:4, or to a fragment of the nucleotide sequence of SEQ ID NO:4, said fragment being at least 15 nucleotides in length.
6. The nucleic acid of claim 5, said nucleic acid having at least 85%
sequence identity to the nucleotide sequence of SEQ ID NO:4 or to said fragment.
sequence identity to the nucleotide sequence of SEQ ID NO:4 or to said fragment.
7. The nucleic acid of claim 5, said nucleic acid having at least 90%
sequence identity to the nucleotide sequence of SEQ ID NO:4 or to said fragment.
sequence identity to the nucleotide sequence of SEQ ID NO:4 or to said fragment.
8. The nucleic acid of claim 5, said nucleic acid having at least 95%
sequence identity to the nucleotide sequence of SEQ ID NO:4 or to said fragment.
sequence identity to the nucleotide sequence of SEQ ID NO:4 or to said fragment.
9. An isolated nucleic acid comprising a first and second region, said first region having at least 80% sequence identity to the nucleotide sequence of SEQ
ID
NO:3, said second region having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:4.
ID
NO:3, said second region having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:4.
10. The nucleic acid of claim 9, wherein said nucleic acid encodes a diacylglycerol acyltransferase polypeptide.
11. The nucleic acid of claim 9, wherein said first and second regions are separated by approximately 600 nucleotides.
12. The nucleic acid of claim 9, wherein said nucleic acid is the insert of pMB 143.
13. An expression vector comprising the nucleic acid of claim 9 operably linked to an expression control element.
14. The expression vector of claim 13, wherein said nucleic acid is operably linked in antisense orientation.
15. A transgenic plant comprising an exogenous nucleic acid encoding a diacylglycerol acyltransferase polypeptide operably linked to a regulatory element
16. The transgenic plant of claim 15, said nucleic acid comprising a first and second region, said first region having at least 80% sequence identity to the nucleotide sequence of SEQ ID N:3, said second region having at least 80%
sequence identity to the nucleotide sequence of SEQ ID NO:4.
sequence identity to the nucleotide sequence of SEQ ID NO:4.
17. The transgenic plant of claim 15, wherein said plant produces seeds that exhibit a statistically significantly altered oil content as compared to seeds produced by a corresponding plant lacking said nucleic acid encoding said diacylglycerol acyltransferase polypeptide.
18. The plant of claim 15, wherein said plant is a soybean plant.
19. The plant of claim 15, wherein said plant is a Brarsica plant.
20. Seeds produced by the plant of claim 15.
21. Progeny of the plant of claim 15.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13241799P | 1999-05-04 | 1999-05-04 | |
US60/132,417 | 1999-05-04 | ||
PCT/US2000/012316 WO2000066749A1 (en) | 1999-05-04 | 2000-05-04 | Plant acyltransferases |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2372632A1 true CA2372632A1 (en) | 2000-11-09 |
CA2372632C CA2372632C (en) | 2011-02-15 |
Family
ID=22453962
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2372632A Expired - Fee Related CA2372632C (en) | 1999-05-04 | 2000-05-04 | Plant acyltransferases |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1173583A1 (en) |
AU (1) | AU784352B2 (en) |
CA (1) | CA2372632C (en) |
WO (1) | WO2000066749A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005052162A1 (en) * | 2003-11-25 | 2005-06-09 | National Research Council Of Canada | Fatty acid elongase (fae) genes and their utility in increasing erucic acid and other very long-chain fatty acid proportions in seed oil. |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020119138A1 (en) | 2001-02-26 | 2002-08-29 | Sylvaine Cases | Diacylglycerol o-acyltransferase 2alpha (DGAT2alpha) |
US7045326B2 (en) | 2001-02-23 | 2006-05-16 | The Regents Of The University Of California | Mono- and diacylglycerol acyltransferases and methods of use thereof |
MXPA05001239A (en) | 2002-07-31 | 2005-06-08 | Monsanto Technology Llc | Diacylglycerol acyltransferase nucleic acid sequences and associated products. |
US20040133944A1 (en) * | 2003-01-08 | 2004-07-08 | Delta And Pine Land Company | Seed oil suppression to enhance yield of commercially important macromolecules |
WO2008006171A1 (en) | 2006-07-14 | 2008-01-17 | Commonwealth Scientific And Industrial Research Organisation | Altering the fatty acid composition of rice |
UA107914C2 (en) | 2008-05-23 | 2015-03-10 | Е. І. Дю Пон Де Немур Енд Компані | Dgat genes from oil organisms to increase production of spare lipid profiles of seeds and modified fatty acids in oleaginous plants |
AU2009273754B2 (en) | 2008-07-21 | 2016-07-14 | Commonwealth Scientific And Industrial Research Organisation | Improved cottonseed oil and uses |
AU2009273755A1 (en) | 2008-07-21 | 2010-01-28 | Commonwealth Scientific And Industrial Research Organisation | Improved vegetable oils and uses therefor |
US9127288B2 (en) | 2010-06-28 | 2015-09-08 | Commonwealth Scientific And Industrial Research Organisation | Methods of producing lipids |
MY188956A (en) | 2011-12-27 | 2022-01-14 | Commw Scient Ind Res Org | Processes for producing lipids |
US8809026B2 (en) | 2011-12-27 | 2014-08-19 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing lipids |
US11639507B2 (en) | 2011-12-27 | 2023-05-02 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing lipids |
UA116538C2 (en) | 2012-04-25 | 2018-04-10 | Коммонвелт Сайнтіфік Енд Індастріел Рісерч Організейшн | High oleic acid oils |
WO2016004473A1 (en) | 2014-07-07 | 2016-01-14 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing industrial products from plant lipids |
US11859193B2 (en) | 2016-09-02 | 2024-01-02 | Nuseed Global Innovation Ltd. | Plants with modified traits |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1266460A (en) * | 1997-06-05 | 2000-09-13 | 卡尔金有限责任公司 | Diacrylglycerol acyl transferase proteins |
DE69834246T2 (en) * | 1997-06-05 | 2007-02-01 | Calgene Llc, Davis | FAT ACYL-COA: FAT ALCOHOL O-ACYL TRANSFERASES |
WO1999063096A2 (en) * | 1998-06-05 | 1999-12-09 | Calgene Llc | Acyl coa:cholesterol acyltransferase related nucleic acid sequences |
DE69939426D1 (en) * | 1998-06-24 | 2008-10-09 | David Gladstone Inst | DIACYLGLYCERINE O-ACYLTRANSFERASE |
US6344548B1 (en) * | 1998-06-24 | 2002-02-05 | The Regents Of The University Of California | Diacylglycerol o-acyltransferase |
DE69941390D1 (en) * | 1998-07-02 | 2009-10-22 | Calgene Llc | DIACYLGLYZERINE ACYLTRANSFERASE PROTEINS |
AU1837600A (en) * | 1998-12-02 | 2000-06-19 | E.I. Du Pont De Nemours And Company | Plant diacylglycerol acyltransferases |
AU2166400A (en) * | 1998-12-04 | 2000-06-19 | Calgene Llc | Diacylglycerol acyl transferase proteins |
DE69933391T2 (en) * | 1998-12-17 | 2007-11-15 | National Research Council Of Canada, Ottawa | DIACYLGLYZERINE ACYLTRANSFERASE GENE FROM PLANTS |
-
2000
- 2000-05-04 CA CA2372632A patent/CA2372632C/en not_active Expired - Fee Related
- 2000-05-04 AU AU47034/00A patent/AU784352B2/en not_active Ceased
- 2000-05-04 WO PCT/US2000/012316 patent/WO2000066749A1/en active Application Filing
- 2000-05-04 EP EP00928864A patent/EP1173583A1/en not_active Withdrawn
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005052162A1 (en) * | 2003-11-25 | 2005-06-09 | National Research Council Of Canada | Fatty acid elongase (fae) genes and their utility in increasing erucic acid and other very long-chain fatty acid proportions in seed oil. |
Also Published As
Publication number | Publication date |
---|---|
WO2000066749A1 (en) | 2000-11-09 |
EP1173583A1 (en) | 2002-01-23 |
AU784352B2 (en) | 2006-03-16 |
AU4703400A (en) | 2000-11-17 |
CA2372632C (en) | 2011-02-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20170504 |