CA2372632A1 - Plant acyltransferases - Google Patents

Plant acyltransferases Download PDF

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Publication number
CA2372632A1
CA2372632A1 CA002372632A CA2372632A CA2372632A1 CA 2372632 A1 CA2372632 A1 CA 2372632A1 CA 002372632 A CA002372632 A CA 002372632A CA 2372632 A CA2372632 A CA 2372632A CA 2372632 A1 CA2372632 A1 CA 2372632A1
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CA
Canada
Prior art keywords
nucleic acid
seq
nucleotide sequence
plant
fragment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002372632A
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French (fr)
Other versions
CA2372632C (en
Inventor
Basil S. Shorrosh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cargill Inc
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2372632A1 publication Critical patent/CA2372632A1/en
Application granted granted Critical
Publication of CA2372632C publication Critical patent/CA2372632C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A gene encoding diacylglycerol acyltransferase from Brassica napus is described, as well as methods of altering oil content in plants using the diacylglycerol acyltransferase.

Claims (21)

1. An isolated nucleic acid having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:3, or to a fragment of the nucleotide sequence of SEQ ID NO:3, said fragment being at least 15 nucleotides in length.
2. The nucleic acid of claim 1, said nucleic acid having at least 85%
sequence identity to the nucleotide sequence of SEQ ID NO:3 or to said fragment.
3. The nucleic acid of claim 1, said nucleic acid having at least 90%
sequence identity to the nucleotide sequence of SEQ ID NO:3 or to said fragment.
4. The nucleic acid of claim 1, said nucleic acid having at least 95%
sequence identity to the nucleotide sequence of SEQ ID NO:3 or to said fragment.
5. An isolated nucleic acid having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:4, or to a fragment of the nucleotide sequence of SEQ ID NO:4, said fragment being at least 15 nucleotides in length.
6. The nucleic acid of claim 5, said nucleic acid having at least 85%
sequence identity to the nucleotide sequence of SEQ ID NO:4 or to said fragment.
7. The nucleic acid of claim 5, said nucleic acid having at least 90%
sequence identity to the nucleotide sequence of SEQ ID NO:4 or to said fragment.
8. The nucleic acid of claim 5, said nucleic acid having at least 95%
sequence identity to the nucleotide sequence of SEQ ID NO:4 or to said fragment.
9. An isolated nucleic acid comprising a first and second region, said first region having at least 80% sequence identity to the nucleotide sequence of SEQ
ID
NO:3, said second region having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:4.
10. The nucleic acid of claim 9, wherein said nucleic acid encodes a diacylglycerol acyltransferase polypeptide.
11. The nucleic acid of claim 9, wherein said first and second regions are separated by approximately 600 nucleotides.
12. The nucleic acid of claim 9, wherein said nucleic acid is the insert of pMB 143.
13. An expression vector comprising the nucleic acid of claim 9 operably linked to an expression control element.
14. The expression vector of claim 13, wherein said nucleic acid is operably linked in antisense orientation.
15. A transgenic plant comprising an exogenous nucleic acid encoding a diacylglycerol acyltransferase polypeptide operably linked to a regulatory element
16. The transgenic plant of claim 15, said nucleic acid comprising a first and second region, said first region having at least 80% sequence identity to the nucleotide sequence of SEQ ID N:3, said second region having at least 80%
sequence identity to the nucleotide sequence of SEQ ID NO:4.
17. The transgenic plant of claim 15, wherein said plant produces seeds that exhibit a statistically significantly altered oil content as compared to seeds produced by a corresponding plant lacking said nucleic acid encoding said diacylglycerol acyltransferase polypeptide.
18. The plant of claim 15, wherein said plant is a soybean plant.
19. The plant of claim 15, wherein said plant is a Brarsica plant.
20. Seeds produced by the plant of claim 15.
21. Progeny of the plant of claim 15.
CA2372632A 1999-05-04 2000-05-04 Plant acyltransferases Expired - Fee Related CA2372632C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13241799P 1999-05-04 1999-05-04
US60/132,417 1999-05-04
PCT/US2000/012316 WO2000066749A1 (en) 1999-05-04 2000-05-04 Plant acyltransferases

Publications (2)

Publication Number Publication Date
CA2372632A1 true CA2372632A1 (en) 2000-11-09
CA2372632C CA2372632C (en) 2011-02-15

Family

ID=22453962

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2372632A Expired - Fee Related CA2372632C (en) 1999-05-04 2000-05-04 Plant acyltransferases

Country Status (4)

Country Link
EP (1) EP1173583A1 (en)
AU (1) AU784352B2 (en)
CA (1) CA2372632C (en)
WO (1) WO2000066749A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005052162A1 (en) * 2003-11-25 2005-06-09 National Research Council Of Canada Fatty acid elongase (fae) genes and their utility in increasing erucic acid and other very long-chain fatty acid proportions in seed oil.

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020119138A1 (en) 2001-02-26 2002-08-29 Sylvaine Cases Diacylglycerol o-acyltransferase 2alpha (DGAT2alpha)
US7045326B2 (en) 2001-02-23 2006-05-16 The Regents Of The University Of California Mono- and diacylglycerol acyltransferases and methods of use thereof
MXPA05001239A (en) 2002-07-31 2005-06-08 Monsanto Technology Llc Diacylglycerol acyltransferase nucleic acid sequences and associated products.
US20040133944A1 (en) * 2003-01-08 2004-07-08 Delta And Pine Land Company Seed oil suppression to enhance yield of commercially important macromolecules
WO2008006171A1 (en) 2006-07-14 2008-01-17 Commonwealth Scientific And Industrial Research Organisation Altering the fatty acid composition of rice
UA107914C2 (en) 2008-05-23 2015-03-10 Е. І. Дю Пон Де Немур Енд Компані Dgat genes from oil organisms to increase production of spare lipid profiles of seeds and modified fatty acids in oleaginous plants
AU2009273754B2 (en) 2008-07-21 2016-07-14 Commonwealth Scientific And Industrial Research Organisation Improved cottonseed oil and uses
AU2009273755A1 (en) 2008-07-21 2010-01-28 Commonwealth Scientific And Industrial Research Organisation Improved vegetable oils and uses therefor
US9127288B2 (en) 2010-06-28 2015-09-08 Commonwealth Scientific And Industrial Research Organisation Methods of producing lipids
MY188956A (en) 2011-12-27 2022-01-14 Commw Scient Ind Res Org Processes for producing lipids
US8809026B2 (en) 2011-12-27 2014-08-19 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
US11639507B2 (en) 2011-12-27 2023-05-02 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
UA116538C2 (en) 2012-04-25 2018-04-10 Коммонвелт Сайнтіфік Енд Індастріел Рісерч Організейшн High oleic acid oils
WO2016004473A1 (en) 2014-07-07 2016-01-14 Commonwealth Scientific And Industrial Research Organisation Processes for producing industrial products from plant lipids
US11859193B2 (en) 2016-09-02 2024-01-02 Nuseed Global Innovation Ltd. Plants with modified traits

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1266460A (en) * 1997-06-05 2000-09-13 卡尔金有限责任公司 Diacrylglycerol acyl transferase proteins
DE69834246T2 (en) * 1997-06-05 2007-02-01 Calgene Llc, Davis FAT ACYL-COA: FAT ALCOHOL O-ACYL TRANSFERASES
WO1999063096A2 (en) * 1998-06-05 1999-12-09 Calgene Llc Acyl coa:cholesterol acyltransferase related nucleic acid sequences
DE69939426D1 (en) * 1998-06-24 2008-10-09 David Gladstone Inst DIACYLGLYCERINE O-ACYLTRANSFERASE
US6344548B1 (en) * 1998-06-24 2002-02-05 The Regents Of The University Of California Diacylglycerol o-acyltransferase
DE69941390D1 (en) * 1998-07-02 2009-10-22 Calgene Llc DIACYLGLYZERINE ACYLTRANSFERASE PROTEINS
AU1837600A (en) * 1998-12-02 2000-06-19 E.I. Du Pont De Nemours And Company Plant diacylglycerol acyltransferases
AU2166400A (en) * 1998-12-04 2000-06-19 Calgene Llc Diacylglycerol acyl transferase proteins
DE69933391T2 (en) * 1998-12-17 2007-11-15 National Research Council Of Canada, Ottawa DIACYLGLYZERINE ACYLTRANSFERASE GENE FROM PLANTS

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005052162A1 (en) * 2003-11-25 2005-06-09 National Research Council Of Canada Fatty acid elongase (fae) genes and their utility in increasing erucic acid and other very long-chain fatty acid proportions in seed oil.

Also Published As

Publication number Publication date
WO2000066749A1 (en) 2000-11-09
EP1173583A1 (en) 2002-01-23
AU784352B2 (en) 2006-03-16
AU4703400A (en) 2000-11-17
CA2372632C (en) 2011-02-15

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