WO2002036786A3 - Method of selecting plant promoters to control transgene expression - Google Patents

Method of selecting plant promoters to control transgene expression Download PDF

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Publication number
WO2002036786A3
WO2002036786A3 PCT/CA2001/001532 CA0101532W WO0236786A3 WO 2002036786 A3 WO2002036786 A3 WO 2002036786A3 CA 0101532 W CA0101532 W CA 0101532W WO 0236786 A3 WO0236786 A3 WO 0236786A3
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WO
WIPO (PCT)
Prior art keywords
promoter
transformed
plants
coding sequence
transgene expression
Prior art date
Application number
PCT/CA2001/001532
Other languages
French (fr)
Other versions
WO2002036786A2 (en
Inventor
Louis-Philippe Vezina
Aoust Marc-Andre D
Francois Arcand
Pierre Bilodeau
Original Assignee
Medicago Inc
Louis-Philippe Vezina
Aoust Marc-Andre D
Francois Arcand
Pierre Bilodeau
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medicago Inc, Louis-Philippe Vezina, Aoust Marc-Andre D, Francois Arcand, Pierre Bilodeau filed Critical Medicago Inc
Priority to AU2002210310A priority Critical patent/AU2002210310A1/en
Publication of WO2002036786A2 publication Critical patent/WO2002036786A2/en
Publication of WO2002036786A3 publication Critical patent/WO2002036786A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for the identification and cloning of promoters that are useful in regulation of gene expression under different environmental conditions, such as in cultured transformed cells or in transgenic plants. A promoter that is a nucleic acid region located upstream of the 5' end of a plant DNA structural coding sequence that is transcribed at desired and/or modulated levels in plant tissues. The promoter regions are capable of conferring high levels of transcription in leaf tissue and in developing seed tissues when used as a promoter for a heterologous coding sequence in a chimeric gene. The promoter and any chimeric gene in which it may be used can be used to obtain transformed plant cells and plants. Chimeric genes including the isolated promoter region, transformed plants containing the isolated promoter region, transformed plant cells and seeds are also disclosed.
PCT/CA2001/001532 2000-10-31 2001-10-31 Method of selecting plant promoters to control transgene expression WO2002036786A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002210310A AU2002210310A1 (en) 2000-10-31 2001-10-31 Method of selecting plant promoters to control transgene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24421400P 2000-10-31 2000-10-31
US60/244,214 2000-10-31

Publications (2)

Publication Number Publication Date
WO2002036786A2 WO2002036786A2 (en) 2002-05-10
WO2002036786A3 true WO2002036786A3 (en) 2002-09-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2001/001532 WO2002036786A2 (en) 2000-10-31 2001-10-31 Method of selecting plant promoters to control transgene expression

Country Status (2)

Country Link
AU (1) AU2002210310A1 (en)
WO (1) WO2002036786A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004532647A (en) 2001-06-14 2004-10-28 スローン−ケターリング インスティチュート フォー キャンサー リサーチ HDAC9 polypeptides and polynucleotides and uses thereof
US7598430B2 (en) 2002-03-20 2009-10-06 J.R. Simplot Company Refined plant transformation
JP3979431B2 (en) * 2003-07-31 2007-09-19 本田技研工業株式会社 Genes that confer plant regeneration ability and use thereof
US7449335B2 (en) 2005-07-27 2008-11-11 J.R. Simplot Company Precise cutting
CA2649134C (en) 2006-02-09 2013-07-02 Thomas Paccalet Synthesis of sialic acid in plants
CN101070536B (en) * 2006-05-11 2012-05-16 中国农业科学院生物技术研究所 Inducing expression of hepatitis-B virus cytomembrane small-protein and middle-protein gene in clover
EP2171068A4 (en) * 2007-06-15 2010-07-14 Medicago Inc Protein production in plants
US9145562B2 (en) 2009-11-20 2015-09-29 Alberta Innovates—Technology Futures Variegation in plants
CN117683807A (en) * 2023-12-16 2024-03-12 韶关学院 Efficient fragment assembly and rapid plant cell protoplast transient expression method, system and application

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997044448A1 (en) * 1996-05-17 1997-11-27 Pioneer Hi-Bred International, Inc. Promoter elements conferring root-preferred gene expression
FR2775000A1 (en) * 1998-02-13 1999-08-20 Lvmh Rech INDUCIBLE PROMOTER IN PLANTS, SEQUENCE INCORPORATING THIS PROMOTER AND PRODUCT OBTAINED
WO2001025455A2 (en) * 1999-10-04 2001-04-12 Medicago Inc. Promoter for regulating expression of foreign genes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997044448A1 (en) * 1996-05-17 1997-11-27 Pioneer Hi-Bred International, Inc. Promoter elements conferring root-preferred gene expression
FR2775000A1 (en) * 1998-02-13 1999-08-20 Lvmh Rech INDUCIBLE PROMOTER IN PLANTS, SEQUENCE INCORPORATING THIS PROMOTER AND PRODUCT OBTAINED
WO2001025455A2 (en) * 1999-10-04 2001-04-12 Medicago Inc. Promoter for regulating expression of foreign genes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHONG S ET AL: "Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 192, no. 2, 1997, pages 271 - 281, XP004081721, ISSN: 0378-1119 *
FORS L ET AL: "CLONING OF THE SHARK PO PROMOTER USING A GENOMIC WALKING TECHNIQUE BASED ON THE POLYMERASE CHAIN REACTION", NUCLEIC ACIDS RESEARCH, vol. 18, no. 9, 1990, pages 2793 - 2800, XP001074204, ISSN: 0305-1048 *
KENG-HOCK PWEE ET AL: "THE PEA PLASTOCYANIN PROMOTER DIRECTS CELL-SPECIFIC BUT NOT FULL LIGHT-REGULATED EXPRESSION IN TRANSGENIC TOBACCO PLANTS", PLANT JOURNAL, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 3, no. 3, 1993, pages 437 - 449, XP002032746, ISSN: 0960-7412 *
STRATER TIM ET AL: "Identification of light- and nitrate-responsive regions of the nitrate reductase promoter from birch.", PLANT SCIENCE (SHANNON), vol. 150, no. 2, 28 January 2000 (2000-01-28), pages 153 - 161, XP002208039, ISSN: 0168-9452 *

Also Published As

Publication number Publication date
WO2002036786A2 (en) 2002-05-10
AU2002210310A1 (en) 2002-05-15

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