MD2312B2 - Succesiune nucleotidă izolată a promotorului genei sintazei acetohidroxiacizilor; vector; procedeu de expresie a genei heteroloage; construct de acid nucleic şi metodă de selecţie a materialului vegetal transgenic - Google Patents

Succesiune nucleotidă izolată a promotorului genei sintazei acetohidroxiacizilor; vector; procedeu de expresie a genei heteroloage; construct de acid nucleic şi metodă de selecţie a materialului vegetal transgenic

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Publication number
MD2312B2
MD2312B2 MD97-0144A MD970144A MD2312B2 MD 2312 B2 MD2312 B2 MD 2312B2 MD 970144 A MD970144 A MD 970144A MD 2312 B2 MD2312 B2 MD 2312B2
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MD
Moldova
Prior art keywords
nucleotide sequence
selection
transgenic
vector
nucleic acid
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MD97-0144A
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English (en)
Russian (ru)
Inventor
Габриель ДИТРИХ
Джейн СМИТ
Джаньинг ПЕНГ
Original Assignee
Эмерикэн Сайенэмид Компэни
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Application filed by Эмерикэн Сайенэмид Компэни filed Critical Эмерикэн Сайенэмид Компэни
Publication of MD2312B2 publication Critical patent/MD2312B2/ro

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Invenţia se referă la ingineria genetică, în particular la o succesiune nucleotidă izolată a promotorului genei sintazei acetohidroxiacizilor şi poate fi utilizată pentru selecţia plantelor.Succesiunea nucleotidă izolată a promotorului genei sintazei acetohidroxiacizilor pentru expresia genelor în plante este selectată din grupa succesiunilor SEQ ID nr. 1, SEQ ID nr. 2 sau SEQ ID nr. 3. Se propune, de asemenea, un vector de transformare a plantelor, care conţine succesiunea nucleotidă menţionată, un procedeu de expresie de un nivel înalt a genei heteroloage în plantă şi în diverse ţesuturi ale acestei plante, un construct de acid nucleic care conţine succesiunea nucleotidă, conjugată cuogenă heteroloagă, şi o metodă de selecţie a materialului vegetal transgenic, rezistent la erbicide.
MD97-0144A 1994-09-08 1995-09-05 Succesiune nucleotidă izolată a promotorului genei sintazei acetohidroxiacizilor; vector; procedeu de expresie a genei heteroloage; construct de acid nucleic şi metodă de selecţie a materialului vegetal transgenic MD2312B2 (ro)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/303,266 US5750866A (en) 1994-09-08 1994-09-08 AHAS promoter useful for expression of introduced genes in plants
PCT/US1995/011199 WO1996007746A1 (en) 1994-09-08 1995-09-05 Acetohydroxyacid synthase promoter expression of introduced genes in plants

Publications (1)

Publication Number Publication Date
MD2312B2 true MD2312B2 (ro) 2003-11-30

Family

ID=23171273

Family Applications (1)

Application Number Title Priority Date Filing Date
MD97-0144A MD2312B2 (ro) 1994-09-08 1995-09-05 Succesiune nucleotidă izolată a promotorului genei sintazei acetohidroxiacizilor; vector; procedeu de expresie a genei heteroloage; construct de acid nucleic şi metodă de selecţie a materialului vegetal transgenic

Country Status (26)

Country Link
US (2) US5750866A (ro)
EP (1) EP0779928B1 (ro)
JP (1) JP3655922B2 (ro)
KR (1) KR970705637A (ro)
CN (1) CN1210404C (ro)
AT (1) ATE291631T1 (ro)
AU (1) AU701239B2 (ro)
BG (1) BG64062B1 (ro)
BR (1) BR9509202A (ro)
CA (1) CA2198497C (ro)
CZ (1) CZ69597A3 (ro)
DE (1) DE69534101T2 (ro)
EE (1) EE03933B1 (ro)
ES (1) ES2240978T3 (ro)
FI (1) FI119818B (ro)
HU (1) HU221795B1 (ro)
MD (1) MD2312B2 (ro)
MX (1) MX9701684A (ro)
NO (1) NO323171B1 (ro)
NZ (1) NZ293093A (ro)
PL (1) PL188062B1 (ro)
RO (1) RO117190B1 (ro)
RU (1) RU2197527C2 (ro)
SK (1) SK30697A3 (ro)
UA (1) UA48951C2 (ro)
WO (1) WO1996007746A1 (ro)

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Also Published As

Publication number Publication date
ATE291631T1 (de) 2005-04-15
NO323171B1 (no) 2007-01-15
NO971069L (no) 1997-05-07
DE69534101T2 (de) 2006-03-23
US5750866A (en) 1998-05-12
EP0779928B1 (en) 2005-03-23
CZ69597A3 (en) 1997-09-17
EE03933B1 (et) 2002-12-16
RO117190B1 (ro) 2001-11-30
AU701239B2 (en) 1999-01-21
BG101270A (en) 1997-10-31
JP3655922B2 (ja) 2005-06-02
BG64062B1 (bg) 2003-11-28
BR9509202A (pt) 1997-12-30
CN1157637A (zh) 1997-08-20
KR970705637A (ko) 1997-10-09
RU2197527C2 (ru) 2003-01-27
CN1210404C (zh) 2005-07-13
MX9701684A (es) 1997-06-28
PL319053A1 (en) 1997-07-21
US6025541A (en) 2000-02-15
HU221795B1 (hu) 2003-01-28
WO1996007746A1 (en) 1996-03-14
NO971069D0 (no) 1997-03-07
ES2240978T3 (es) 2005-10-16
CA2198497C (en) 2009-11-03
FI970958A0 (fi) 1997-03-06
HUT77109A (hu) 1998-03-02
JPH10504968A (ja) 1998-05-19
FI970958A (fi) 1997-03-06
DE69534101D1 (de) 2005-04-28
PL188062B1 (pl) 2004-12-31
UA48951C2 (uk) 2002-09-16
FI119818B (fi) 2009-03-31
EP0779928A1 (en) 1997-06-25
EE9700213A (et) 1998-02-16
SK30697A3 (en) 1997-12-10
CA2198497A1 (en) 1996-03-14
AU3544895A (en) 1996-03-27
NZ293093A (en) 1999-01-28

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