UA48951C2 - Ізольований фрагмент днк промотору гена синтази ацетооксикислот (ahas) для експресії генів в рослинах, вектор для трансформації рослин, спосіб високорівневого експресування гетерологічного гена в рослині та в різноманітних тканинах цієї рослини, конструкція нуклеїнової кислоти, спосіб селекції трансгенного рослинного матеріалу - Google Patents

Ізольований фрагмент днк промотору гена синтази ацетооксикислот (ahas) для експресії генів в рослинах, вектор для трансформації рослин, спосіб високорівневого експресування гетерологічного гена в рослині та в різноманітних тканинах цієї рослини, конструкція нуклеїнової кислоти, спосіб селекції трансгенного рослинного матеріалу

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Publication number
UA48951C2
UA48951C2 UA97041679A UA97041679A UA48951C2 UA 48951 C2 UA48951 C2 UA 48951C2 UA 97041679 A UA97041679 A UA 97041679A UA 97041679 A UA97041679 A UA 97041679A UA 48951 C2 UA48951 C2 UA 48951C2
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Ukraine
Prior art keywords
plants
plant
ahas
heterologous gene
transformation
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UA97041679A
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English (en)
Russian (ru)
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Габріель Дітріх
Джейн Сміт
Джіань Їнг. Пенг
Original Assignee
Америкен Ціанамід Компані
Америкен Цианамид Компани
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23171273&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=UA48951(C2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Америкен Ціанамід Компані, Америкен Цианамид Компани filed Critical Америкен Ціанамід Компані
Publication of UA48951C2 publication Critical patent/UA48951C2/uk

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Цей винахід відноситься до ізольованих фрагментів ДНК, що не кодують, корисним для використання в якості промоторів для експресії гетерологічних генів у рослин. Дійсний винахід також відноситься до векторів, що включають ці ізольовані нуклеотидні послідовності. Для експресії інтродуційованих генів на високому рівні та у різних тканинах рослин використовуються промотори гена синтази ацетооксикислот (AHAS) з кукурудзи. Були клоновані і секвеновані промотори з als1 та als2 генів кукурудзи, і регіони промоторів цих генів потім введені в плазміду 5' до гена-репортера β-глюкуронідази (GUS). Обидва фрагменти промотору походять з лінії кукурудзи XI12. Фрагмент промотору als1 довжиною приблизно 1400 пар основ, у той час як промотор als2 містить 819 пар основ.
UA97041679A 1994-09-08 1995-09-05 Ізольований фрагмент днк промотору гена синтази ацетооксикислот (ahas) для експресії генів в рослинах, вектор для трансформації рослин, спосіб високорівневого експресування гетерологічного гена в рослині та в різноманітних тканинах цієї рослини, конструкція нуклеїнової кислоти, спосіб селекції трансгенного рослинного матеріалу UA48951C2 (uk)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/303,266 US5750866A (en) 1994-09-08 1994-09-08 AHAS promoter useful for expression of introduced genes in plants
PCT/US1995/011199 WO1996007746A1 (en) 1994-09-08 1995-09-05 Acetohydroxyacid synthase promoter expression of introduced genes in plants

Publications (1)

Publication Number Publication Date
UA48951C2 true UA48951C2 (uk) 2002-09-16

Family

ID=23171273

Family Applications (1)

Application Number Title Priority Date Filing Date
UA97041679A UA48951C2 (uk) 1994-09-08 1995-09-05 Ізольований фрагмент днк промотору гена синтази ацетооксикислот (ahas) для експресії генів в рослинах, вектор для трансформації рослин, спосіб високорівневого експресування гетерологічного гена в рослині та в різноманітних тканинах цієї рослини, конструкція нуклеїнової кислоти, спосіб селекції трансгенного рослинного матеріалу

Country Status (26)

Country Link
US (2) US5750866A (uk)
EP (1) EP0779928B1 (uk)
JP (1) JP3655922B2 (uk)
KR (1) KR970705637A (uk)
CN (1) CN1210404C (uk)
AT (1) ATE291631T1 (uk)
AU (1) AU701239B2 (uk)
BG (1) BG64062B1 (uk)
BR (1) BR9509202A (uk)
CA (1) CA2198497C (uk)
CZ (1) CZ69597A3 (uk)
DE (1) DE69534101T2 (uk)
EE (1) EE03933B1 (uk)
ES (1) ES2240978T3 (uk)
FI (1) FI119818B (uk)
HU (1) HU221795B1 (uk)
MD (1) MD2312B2 (uk)
MX (1) MX9701684A (uk)
NO (1) NO323171B1 (uk)
NZ (1) NZ293093A (uk)
PL (1) PL188062B1 (uk)
RO (1) RO117190B1 (uk)
RU (1) RU2197527C2 (uk)
SK (1) SK30697A3 (uk)
UA (1) UA48951C2 (uk)
WO (1) WO1996007746A1 (uk)

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Also Published As

Publication number Publication date
EP0779928A1 (en) 1997-06-25
US6025541A (en) 2000-02-15
SK30697A3 (en) 1997-12-10
CZ69597A3 (en) 1997-09-17
AU3544895A (en) 1996-03-27
EE9700213A (et) 1998-02-16
RU2197527C2 (ru) 2003-01-27
AU701239B2 (en) 1999-01-21
BG101270A (en) 1997-10-31
JPH10504968A (ja) 1998-05-19
MD2312B2 (ro) 2003-11-30
CN1157637A (zh) 1997-08-20
HUT77109A (hu) 1998-03-02
MX9701684A (es) 1997-06-28
CA2198497A1 (en) 1996-03-14
FI119818B (fi) 2009-03-31
FI970958A (fi) 1997-03-06
CN1210404C (zh) 2005-07-13
PL188062B1 (pl) 2004-12-31
JP3655922B2 (ja) 2005-06-02
EP0779928B1 (en) 2005-03-23
NO971069D0 (no) 1997-03-07
KR970705637A (ko) 1997-10-09
EE03933B1 (et) 2002-12-16
ES2240978T3 (es) 2005-10-16
ATE291631T1 (de) 2005-04-15
DE69534101T2 (de) 2006-03-23
RO117190B1 (ro) 2001-11-30
HU221795B1 (hu) 2003-01-28
NZ293093A (en) 1999-01-28
WO1996007746A1 (en) 1996-03-14
NO971069L (no) 1997-05-07
US5750866A (en) 1998-05-12
CA2198497C (en) 2009-11-03
FI970958A0 (fi) 1997-03-06
NO323171B1 (no) 2007-01-15
PL319053A1 (en) 1997-07-21
BR9509202A (pt) 1997-12-30
BG64062B1 (bg) 2003-11-28
DE69534101D1 (de) 2005-04-28

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