RO117190B1 - Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă - Google Patents

Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă

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Publication number
RO117190B1
RO117190B1 RO97-00422A RO9700422A RO117190B1 RO 117190 B1 RO117190 B1 RO 117190B1 RO 9700422 A RO9700422 A RO 9700422A RO 117190 B1 RO117190 B1 RO 117190B1
Authority
RO
Romania
Prior art keywords
plant
expression
identifying
heterologous gene
nucleic acid
Prior art date
Application number
RO97-00422A
Other languages
English (en)
Inventor
Gabriele Dietrich
Jane Smith
Jianying Peng
Original Assignee
American Cyanamid Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23171273&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=RO117190(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by American Cyanamid Company filed Critical American Cyanamid Company
Publication of RO117190B1 publication Critical patent/RO117190B1/ro

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Invenţia se referă la un construct de acid nucleic ce cuprinde un promotor AHAS pentru expresia genelor, în plante, legat operaţional la o secvenţă care codifică o genă heteroloagă, promotor care poate fi selectat dintre promotorii genelor als 1 şi als 2 de la porumb sau promotorul AHAS ce cuprinde secvenţa SEQ ID No 1, SEQ ID No 2 sau SEQ ID No 3, precum şi la o metodă de identificare a expresiei genice, într-o plantă şi/sau în diferite ţesuturi ale acesteia, şi care constă în transformarea recombinantă a unei porţiuni din plantă, expresia genei heteroloage în porţiunea din plantă, astfel transformată, şi detectarea expresiei genice.
RO97-00422A 1994-09-08 1995-09-05 Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă RO117190B1 (ro)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/303,266 US5750866A (en) 1994-09-08 1994-09-08 AHAS promoter useful for expression of introduced genes in plants
PCT/US1995/011199 WO1996007746A1 (en) 1994-09-08 1995-09-05 Acetohydroxyacid synthase promoter expression of introduced genes in plants

Publications (1)

Publication Number Publication Date
RO117190B1 true RO117190B1 (ro) 2001-11-30

Family

ID=23171273

Family Applications (1)

Application Number Title Priority Date Filing Date
RO97-00422A RO117190B1 (ro) 1994-09-08 1995-09-05 Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă

Country Status (26)

Country Link
US (2) US5750866A (ro)
EP (1) EP0779928B1 (ro)
JP (1) JP3655922B2 (ro)
KR (1) KR970705637A (ro)
CN (1) CN1210404C (ro)
AT (1) ATE291631T1 (ro)
AU (1) AU701239B2 (ro)
BG (1) BG64062B1 (ro)
BR (1) BR9509202A (ro)
CA (1) CA2198497C (ro)
CZ (1) CZ69597A3 (ro)
DE (1) DE69534101T2 (ro)
EE (1) EE03933B1 (ro)
ES (1) ES2240978T3 (ro)
FI (1) FI119818B (ro)
HU (1) HU221795B1 (ro)
MD (1) MD2312B2 (ro)
MX (1) MX9701684A (ro)
NO (1) NO323171B1 (ro)
NZ (1) NZ293093A (ro)
PL (1) PL188062B1 (ro)
RO (1) RO117190B1 (ro)
RU (1) RU2197527C2 (ro)
SK (1) SK30697A3 (ro)
UA (1) UA48951C2 (ro)
WO (1) WO1996007746A1 (ro)

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AU2001259206B2 (en) 2000-04-28 2006-05-25 Basf Aktiengesellschaft Use of the maize x112 mutant ahas 2 gene and imidazolinone herbicides for selection of transgenic monocots, maize, rice and wheat plants resistant to the imidazolinone herbicides
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AU2005321630A1 (en) 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
JP2008507960A (ja) 2004-07-31 2008-03-21 メタノミクス ゲーエムベーハー より成長が早いかつ/又はより収量が高い生物の作製
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CN101495507B (zh) 2004-09-24 2013-07-17 巴斯福植物科学有限公司 编码与非生物性胁迫反应相关的蛋白质的核酸序列和具有增加的环境胁迫抗性的植物细胞和植物
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MX2010002931A (es) 2007-09-18 2010-06-01 Basf Plant Science Gmbh Plantas con rendimiento incrementado.
CA2712326A1 (en) 2007-09-21 2009-03-26 Basf Plant Science Gmbh Plants with increased yield comprising increased glutamine synthetase activity
CA2708094A1 (en) * 2007-12-19 2009-06-25 Basf Plant Science Gmbh Plants with increased yield and/or increased tolerance to environmental stress (iy-bm)
DE112008003433T5 (de) 2007-12-21 2010-11-04 Basf Plant Science Gmbh Pflanzen mit erhöhtem Ertrag (KO NUE)
AU2009218478A1 (en) 2008-02-27 2009-09-03 Basf Plant Science Gmbh Plants with increased yield
DE112009001994T5 (de) 2008-08-19 2012-01-19 Basf Plant Science Gmbh Pflanzen mit erhöhtem Ertrag durch Erhöhen oder Erzeugen einer oder mehrerer Aktivitäten in einer Pflanze oder einem Teil davon
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Also Published As

Publication number Publication date
HUT77109A (hu) 1998-03-02
AU701239B2 (en) 1999-01-21
UA48951C2 (uk) 2002-09-16
JPH10504968A (ja) 1998-05-19
NO323171B1 (no) 2007-01-15
FI119818B (fi) 2009-03-31
NO971069D0 (no) 1997-03-07
HU221795B1 (hu) 2003-01-28
NO971069L (no) 1997-05-07
EE9700213A (et) 1998-02-16
BG64062B1 (bg) 2003-11-28
EP0779928B1 (en) 2005-03-23
DE69534101D1 (de) 2005-04-28
US6025541A (en) 2000-02-15
RU2197527C2 (ru) 2003-01-27
CZ69597A3 (en) 1997-09-17
JP3655922B2 (ja) 2005-06-02
EE03933B1 (et) 2002-12-16
BR9509202A (pt) 1997-12-30
AU3544895A (en) 1996-03-27
WO1996007746A1 (en) 1996-03-14
FI970958A0 (fi) 1997-03-06
BG101270A (en) 1997-10-31
MD2312B2 (ro) 2003-11-30
ATE291631T1 (de) 2005-04-15
CA2198497C (en) 2009-11-03
DE69534101T2 (de) 2006-03-23
MX9701684A (es) 1997-06-28
US5750866A (en) 1998-05-12
PL188062B1 (pl) 2004-12-31
KR970705637A (ko) 1997-10-09
EP0779928A1 (en) 1997-06-25
NZ293093A (en) 1999-01-28
CN1157637A (zh) 1997-08-20
CA2198497A1 (en) 1996-03-14
CN1210404C (zh) 2005-07-13
ES2240978T3 (es) 2005-10-16
PL319053A1 (en) 1997-07-21
FI970958L (fi) 1997-03-06
SK30697A3 (en) 1997-12-10

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