WO2001044454A3 - Wurzelspezifischer promotor - Google Patents

Wurzelspezifischer promotor Download PDF

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Publication number
WO2001044454A3
WO2001044454A3 PCT/DE2000/004521 DE0004521W WO0144454A3 WO 2001044454 A3 WO2001044454 A3 WO 2001044454A3 DE 0004521 W DE0004521 W DE 0004521W WO 0144454 A3 WO0144454 A3 WO 0144454A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid sequence
relates
root
seq
Prior art date
Application number
PCT/DE2000/004521
Other languages
English (en)
French (fr)
Other versions
WO2001044454A2 (de
Inventor
Florian Grundler
Inke Nitz
Piotr Puzio
Original Assignee
Das Rektorat Der Christian Alb
Florian Grundler
Inke Nitz
Piotr Puzio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Das Rektorat Der Christian Alb, Florian Grundler, Inke Nitz, Piotr Puzio filed Critical Das Rektorat Der Christian Alb
Priority to JP2001545531A priority Critical patent/JP2003519475A/ja
Priority to CA002396539A priority patent/CA2396539A1/en
Priority to EP00993417A priority patent/EP1244802A2/de
Publication of WO2001044454A2 publication Critical patent/WO2001044454A2/de
Publication of WO2001044454A3 publication Critical patent/WO2001044454A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8227—Root-specific

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die vorliegende Erfindung betrifft transgene Pflanzen mit einer nach ihrer Transformation stabil in das Genom integrierten regulatorischen Nukleinsäuresequenz gemäss SEQ ID NO:1 bis 3, oder ihrem Fragment oder Derivat und einer mit dieser Nukleinsäuresequenz funktionell verbundenen für ein Genprodukt codierenden Nukleinsäuresequenz. Ferner betrifft die vorliegende Erfindung Verfahren zur Herstellung der erfindungsgemässen transgenen Pflanzen sowie die Nukleinsäuresequenzen gemäss SEQ ID NO:1 bis 3. Die regulatorische Nukleinsäuresequenz betrifft Polynukleotide, die natürlicherweise in Pflanzen der Art Arabidopsis thaliana eine weitgehend wurzelspezifische Expression eines Fremdgens erlauben.
PCT/DE2000/004521 1999-12-16 2000-12-18 Wurzelspezifischer promotor WO2001044454A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2001545531A JP2003519475A (ja) 1999-12-16 2000-12-18 根特異的プロモーター
CA002396539A CA2396539A1 (en) 1999-12-16 2000-12-18 Root-specific promoter
EP00993417A EP1244802A2 (de) 1999-12-16 2000-12-18 Wurzelspezifischer promotor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19960843A DE19960843A1 (de) 1999-12-16 1999-12-16 Wurzelspezifischer Promotor
DE19960843.1 1999-12-16

Publications (2)

Publication Number Publication Date
WO2001044454A2 WO2001044454A2 (de) 2001-06-21
WO2001044454A3 true WO2001044454A3 (de) 2001-12-20

Family

ID=7932986

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/004521 WO2001044454A2 (de) 1999-12-16 2000-12-18 Wurzelspezifischer promotor

Country Status (7)

Country Link
US (1) US20030177536A1 (de)
EP (1) EP1244802A2 (de)
JP (1) JP2003519475A (de)
CN (1) CN1434869A (de)
CA (1) CA2396539A1 (de)
DE (1) DE19960843A1 (de)
WO (1) WO2001044454A2 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7078589B2 (en) * 2003-05-02 2006-07-18 Pioneer Hi-Bred International, Inc. Isolated nematode regulated gene promoter and use thereof
US8709811B2 (en) 2003-10-03 2014-04-29 University Of Florida Research Foundation, Inc. Materials and methods for synthesis of a flavor and aroma volatile in plants
KR100604186B1 (ko) 2004-08-25 2006-07-25 고려대학교 산학협력단 고구마 유래 식물 저장뿌리용 고효율 발현 프로모터염기서열, 이를 포함하는 식물체 고효율 일시적 발현벡터및 상기 발현벡터를 이용하여 식물의 저장뿌리에일시적으로 발현시키는 방법
FR2878862A1 (fr) * 2004-12-08 2006-06-09 Genoplante Valor Soc Par Actio Sequences polynucleotidiques a activite promotrice specifique des cellules des racines des plantes
WO2006066193A2 (en) * 2004-12-16 2006-06-22 Ceres, Inc. Root active promoters and uses thereof
MX339202B (es) 2005-04-21 2016-05-13 Univ Florida Materiales y metodos para el control de enfermedades respiratorias en caninos.
US7959929B2 (en) 2005-04-21 2011-06-14 University Of Florida Research Foundation, Inc. Materials and methods for respiratory disease control in canines
US11865172B2 (en) 2005-04-21 2024-01-09 University Of Florida Research Foundation, Inc. Materials and methods for respiratory disease control in canines
WO2006116202A1 (en) * 2005-04-22 2006-11-02 University Of Florida Research Foundation, Inc. Materials and methods for engineering resistance to tomato yellow leaf curl virus (tylcv) in plants
JP2009512449A (ja) 2005-10-19 2009-03-26 ユニバーシティ オブ フロリダ リサーチ ファンデーション インコーポレーティッド イヌにおける呼吸器疾患管理のための材料および方法
WO2009117853A1 (zh) * 2008-03-27 2009-10-01 北京优利康生物农业技术有限公司 一种培育氮吸收能力提高的植物的方法
US9074193B2 (en) * 2008-04-09 2015-07-07 University Of Florida Research Foundation, Inc. Heat resistant plants and plant tissues and methods and materials for making and using same
CN101280313B (zh) * 2008-05-21 2010-06-02 中国农业大学 一种根特异性启动子及其重组表达载体
US8362321B2 (en) * 2009-02-02 2013-01-29 University Of Florida Research Foundation, Inc. Methods and materials for increasing starch biosynthesis in plants
CN102458099B (zh) 2009-06-05 2015-08-19 佛罗里达大学研究基金公司 甘蔗木质素生物合成基因的分离和靶向抑制
AU2010271586B2 (en) 2009-07-16 2016-08-11 Wageningen Universiteit Regulation of zinc deficiency and tolerance in plants
EP2275564A1 (de) * 2009-07-17 2011-01-19 Freie Universität Berlin Mittel und Verfahren zur Produktion von transgenen Pflanzen, die gegenüber Kohlhernie resistent sind
KR101142650B1 (ko) 2009-09-04 2012-05-10 박왕수 다수확성 복분자 산딸기 신품종 식물
KR101069151B1 (ko) 2009-12-08 2011-10-06 한국생명공학연구원 토마토 유래의 뿌리 조직 특이적 프로모터 ＳｌＰＯＤ 및 이의 용도
CN101831431B (zh) * 2010-05-19 2012-05-23 中国科学院遗传与发育生物学研究所 启动子afb4及其应用
CN101914539B (zh) * 2010-08-05 2012-01-25 中国热带农业科学院热带生物技术研究所 一种根部特异性表达启动子及其植物表达载体
KR101303752B1 (ko) 2011-09-26 2013-09-11 대한민국 식물 조직 특이적인 ＢｒＬＲＰ1 프로모터
CN102618543B (zh) * 2012-03-13 2015-01-21 青岛农业大学 一种获自大豆的根特异性兼伤害诱导型启动子
US20130323221A1 (en) 2012-05-30 2013-12-05 Biostrategies LC Plant lectins as carriers of associated drug substances into animal and human cells
KR101450398B1 (ko) 2012-10-12 2014-10-15 경희대학교 산학협력단 뿌리 특이적 프로모터, 이를 포함하는 발현 벡터, 이에 의한 형질 전환 식물체 및 이의 제조방법
WO2014201156A1 (en) 2013-06-11 2014-12-18 Florida State University Research Foundation, Inc. Materials and methods for controlling bundle sheath cell fate and function in plants
KR101485260B1 (ko) * 2013-10-28 2015-01-22 경희대학교 산학협력단 뿌리 특이적 프로모터, 이를 포함하는 발현 벡터, 이에 의한 형질 전환 식물체 및 이의 제조방법
CN104673793B (zh) * 2013-11-27 2017-12-08 中国科学院上海生命科学研究院 豆科植物根系组织特异性启动子及其应用
EP3912635A1 (de) 2014-07-11 2021-11-24 Biostrategies LC Materialien und verfahren zur behandlung von leiden in zusammenhang mit sulfataseenzymen
US10947558B2 (en) * 2015-07-22 2021-03-16 Rutgers, The State University Of New Jersey Compositions and methods for inducing resistance to soybean cyst nematode via RNAi
CN113249390A (zh) * 2021-06-21 2021-08-13 山东省农业科学院 拟南芥agp30基因在降低植物镉吸收中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9524395D0 (en) * 1995-11-29 1996-01-31 Nickerson Biocem Ltd Promoters
WO1999028483A2 (en) * 1997-11-27 1999-06-10 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw. Isolation and characterization of plant regulatory sequences

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 28 June 1999 (1999-06-28), SALANOUBAT ET AL.: "Arabidopsis thaliana genome survey sequence T7 end of BAC T5C7 of TAMU library from strain Columbia of Arabidopsis thaliana", XP002174443 *
DATABASE EMBL 7 October 1999 (1999-10-07), LIN ET AL.: "Arabidopsis thaliana chromosome III BAC F3L24 genomic sequence", XP002174442 *
KAY-PETER SCHMIDT: "Proteinanalytische Charaketrisierung pathogenespezifischer Vorgänge im Wurzelgewebe von Arabidopsis thaliana nach Infektion mit dem Rübenzystennematoden Heterodera schachtii", DISSERTATION AN DER CHRISTIAN-ALBRECHTS-UNIVERSITÄT KIEL, 166 SEITEN, 1995, pages 92 - 150, XP002175067 *
NITZ I ET AL.: "Pyk10, a seedling and root specific gene and promoter from Arabidopsis thaliana", PLANT SCIENCE, ISSN 0168-9452, vol. 161, no. 2, July 2000 (2000-07-01), pages 337 - 346, XP001013257 *
PUZIO P S ET AL: "Isolation of a gene from Arabidopsis thaliana related to nematode feeding structures.", GENE, (1999 OCT 18) 239 (1) 163-72., XP001008756 *
PUZIO PIOTR S ET AL: "Promoter analysis of pyk20, a gene from Arabidopsis thaliana.", PLANT SCIENCE (SHANNON), vol. 157, no. 2, 22 August 2000 (2000-08-22), pages 245 - 255, XP001013126, ISSN: 0168-9452 *

Also Published As

Publication number Publication date
EP1244802A2 (de) 2002-10-02
CA2396539A1 (en) 2001-06-21
CN1434869A (zh) 2003-08-06
DE19960843A1 (de) 2001-06-28
JP2003519475A (ja) 2003-06-24
WO2001044454A2 (de) 2001-06-21
US20030177536A1 (en) 2003-09-18

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