WO2001007590A3 - Chimeric genes for plastid expression - Google Patents

Chimeric genes for plastid expression Download PDF

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Publication number
WO2001007590A3
WO2001007590A3 PCT/EP2000/007118 EP0007118W WO0107590A3 WO 2001007590 A3 WO2001007590 A3 WO 2001007590A3 EP 0007118 W EP0007118 W EP 0007118W WO 0107590 A3 WO0107590 A3 WO 0107590A3
Authority
WO
WIPO (PCT)
Prior art keywords
genes
chimeric genes
plastid
plastid expression
upstream elements
Prior art date
Application number
PCT/EP2000/007118
Other languages
French (fr)
Other versions
WO2001007590A2 (en
Inventor
Peter Bernard Heifetz
Kevin Cox
Original Assignee
Syngenta Participations Ag
Peter Bernard Heifetz
Kevin Cox
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag, Peter Bernard Heifetz, Kevin Cox filed Critical Syngenta Participations Ag
Priority to AU62776/00A priority Critical patent/AU6277600A/en
Publication of WO2001007590A2 publication Critical patent/WO2001007590A2/en
Publication of WO2001007590A3 publication Critical patent/WO2001007590A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters

Abstract

Novel chimeric genes and methods for the control of expression of nucleic acid sequences in plant plastids are described in the instant invention. Multiple genes or polycistronic units are expressed using upstream elements of plastid genes naturally found in divergent orientation. These upstream elements may be derived from sequences found in the native plastid genome of the same plant, or preferably from the plastid genome of a different plant species having sequence identity of less than 90 %.
PCT/EP2000/007118 1999-07-27 2000-07-25 Chimeric genes for plastid expression WO2001007590A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU62776/00A AU6277600A (en) 1999-07-27 2000-07-25 Novel chimeric genes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36181299A 1999-07-27 1999-07-27
US09/361,812 1999-07-27

Publications (2)

Publication Number Publication Date
WO2001007590A2 WO2001007590A2 (en) 2001-02-01
WO2001007590A3 true WO2001007590A3 (en) 2001-07-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/007118 WO2001007590A2 (en) 1999-07-27 2000-07-25 Chimeric genes for plastid expression

Country Status (3)

Country Link
AR (1) AR024934A1 (en)
AU (1) AU6277600A (en)
WO (1) WO2001007590A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030041353A1 (en) 2001-04-18 2003-02-27 Henry Daniell Mutiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
WO2001064024A1 (en) * 2000-02-29 2001-09-07 Auburn University Multiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
CN100335656C (en) * 2004-11-18 2007-09-05 中国农业科学院作物育种栽培研究所 Inspection of wheat polyphenol oxidase activity characteristic and its special primer
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
EP3495486B1 (en) * 2013-12-30 2020-12-16 CureVac AG Artificial nucleic acid molecules
CA2927862C (en) 2013-12-30 2024-01-23 Curevac Ag Artificial nucleic acid molecules
US11254951B2 (en) 2014-12-30 2022-02-22 Curevac Ag Artificial nucleic acid molecules
WO2016091391A1 (en) 2014-12-12 2016-06-16 Curevac Ag Artificial nucleic acid molecules for improved protein expression
EP3260542A1 (en) 2016-06-20 2017-12-27 Algentech Protein production in plant cells

Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995016783A1 (en) * 1993-12-14 1995-06-22 Calgene Inc. Controlled expression of transgenic constructs in plant plastids
WO1995034659A1 (en) * 1994-06-16 1995-12-21 Ciba-Geigy Ag Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
WO1997006250A1 (en) * 1995-08-10 1997-02-20 Rutgers University Nuclear-encoded transcription system in plastids of higher plants
EP0770682A2 (en) * 1995-10-11 1997-05-02 Jinro Limited Herbicide-tolerant transgenic plant
WO1997032011A1 (en) * 1996-02-28 1997-09-04 Novartis Ag Dna molecules encoding plant protoporphyrinogen oxidase and inhibitor-resistant mutants thereof
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
WO1998011235A2 (en) * 1996-09-12 1998-03-19 Novartis Ag Transgenic plants expressing cellulolytic enzymes
WO1998033927A1 (en) * 1997-01-31 1998-08-06 E.I. Du Pont De Nemours And Company Genetically transformed plants demonstrating resistance to porphyrinogen biosynthesis-inhibiting herbicides
WO1998055595A1 (en) * 1997-06-03 1998-12-10 Rutgers, The State University Of New Jersey Plastid promoters for transgene expression in the plastids of higher plants
WO1999005265A2 (en) * 1997-07-23 1999-02-04 Sanford Scientific, Inc. Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
WO1999046370A2 (en) * 1998-03-11 1999-09-16 Novartis Ag Expression of trehalose biosynthetic genes in plants
WO1999046394A1 (en) * 1998-03-11 1999-09-16 Novartis Ag Novel plant plastid promoter sequence
WO2000003017A2 (en) * 1998-07-10 2000-01-20 Calgene Llc Enhancer elements for increased translation in plant plastids
WO2000003022A2 (en) * 1998-07-10 2000-01-20 Calgene Llc Expression of herbicide tolerance genes in plant plastids
WO2000020612A2 (en) * 1998-10-07 2000-04-13 Syngenta Participations Ag Therapeutically active proteins in plants

Patent Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
WO1995016783A1 (en) * 1993-12-14 1995-06-22 Calgene Inc. Controlled expression of transgenic constructs in plant plastids
WO1995034659A1 (en) * 1994-06-16 1995-12-21 Ciba-Geigy Ag Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
WO1997006250A1 (en) * 1995-08-10 1997-02-20 Rutgers University Nuclear-encoded transcription system in plastids of higher plants
EP0770682A2 (en) * 1995-10-11 1997-05-02 Jinro Limited Herbicide-tolerant transgenic plant
WO1997032011A1 (en) * 1996-02-28 1997-09-04 Novartis Ag Dna molecules encoding plant protoporphyrinogen oxidase and inhibitor-resistant mutants thereof
WO1998011235A2 (en) * 1996-09-12 1998-03-19 Novartis Ag Transgenic plants expressing cellulolytic enzymes
WO1998033927A1 (en) * 1997-01-31 1998-08-06 E.I. Du Pont De Nemours And Company Genetically transformed plants demonstrating resistance to porphyrinogen biosynthesis-inhibiting herbicides
WO1998055595A1 (en) * 1997-06-03 1998-12-10 Rutgers, The State University Of New Jersey Plastid promoters for transgene expression in the plastids of higher plants
WO1999005265A2 (en) * 1997-07-23 1999-02-04 Sanford Scientific, Inc. Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
WO1999046370A2 (en) * 1998-03-11 1999-09-16 Novartis Ag Expression of trehalose biosynthetic genes in plants
WO1999046394A1 (en) * 1998-03-11 1999-09-16 Novartis Ag Novel plant plastid promoter sequence
WO2000003017A2 (en) * 1998-07-10 2000-01-20 Calgene Llc Enhancer elements for increased translation in plant plastids
WO2000003022A2 (en) * 1998-07-10 2000-01-20 Calgene Llc Expression of herbicide tolerance genes in plant plastids
WO2000020612A2 (en) * 1998-10-07 2000-04-13 Syngenta Participations Ag Therapeutically active proteins in plants

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 19 November 1998 (1998-11-19), SRIRAMAN P., ET AL.: "Arabidopsis thaliana ATP-dependent protease proteolytic subunit (clpP) gene, promoter region and partial cds.", XP002159239 *
ROGERS S A ET AL: "ANALYSIS OF CHLOROPLAST PROMOTERS USING BIDIRECTIONAL TRANSCRIPTION VECTORS", PLANT MOLECULAR BIOLOGY, vol. 15, no. 3, 1990, pages 421 - 435, XP002159238, ISSN: 0167-4412 *
SRIRAMAN ET AL: "the phage-type PclpP-53 promoter comprises sequences downstream of the transcription inititaion site", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 26, no. 21, November 1998 (1998-11-01), pages 4874 - 4879, XP002106113, ISSN: 0305-1048 *
SRIRAMAN ET AL: "transcription from heterologous rRNA operon promoters in chloroplasts reveals requirement for specific activating factors", PLANT PHYSIOLOGY,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 117, no. 4, August 1998 (1998-08-01), pages 1495 - 1499, XP002106114, ISSN: 0032-0889 *
ZOUBENKO ET AL: "efficient targeting of foreign genes into the tobacco plastid genome", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 22, no. 19, 1994, pages 3819 - 3824, XP002090405, ISSN: 0305-1048 *

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Publication number Publication date
WO2001007590A2 (en) 2001-02-01
AR024934A1 (en) 2002-10-30
AU6277600A (en) 2001-02-13

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