KR950703059A - 폴리프럭탄(레반)을 생성하는 dna 서열, 이들 서열을 포함하는 플라스미드 및 유전자전환 식물의 제조 방법(dna sequences which lead to the formation of polyfructans (levans), plasmids containing these sequences as well as a process for preparing transgenic plants) - Google Patents

폴리프럭탄(레반)을 생성하는 dna 서열, 이들 서열을 포함하는 플라스미드 및 유전자전환 식물의 제조 방법(dna sequences which lead to the formation of polyfructans (levans), plasmids containing these sequences as well as a process for preparing transgenic plants) Download PDF

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KR950703059A
KR950703059A KR1019950700522A KR19950700522A KR950703059A KR 950703059 A KR950703059 A KR 950703059A KR 1019950700522 A KR1019950700522 A KR 1019950700522A KR 19950700522 A KR19950700522 A KR 19950700522A KR 950703059 A KR950703059 A KR 950703059A
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levan
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마누엘라 뢰베르
게바르트 가이어
클라우스 가이더
로타르 빌미처
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로타르 빌미처
인스티투트 퓌르 겐비올로지쉐 포르슝 베를린 게엠베하(Institut Fur Genbiologische Forschung Berlin Gmbh)
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans

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Abstract

본 발명은 폴리프럭탄(레반)을 생성하는 DNA 서열, 및 이들 DNA 서열을 포함하는 플라스미드 뿐만 아닐, 이들 플라스미드를 사용하며 폴리프럭탄(레반)을 발현하는 유전자전환 식물을 제조하기 위한 방법에 관한 것을기재하고 있다.

Description

폴리프럭탄(레반)을 생성하는 DNA 서열, 이들 서열을 포함하는 플라스미드 및 유전자전환 식물의 제조 방법(DNA SEQUENCES WHICH LEAD TO THE FORMATION OF POLYFRUCTANS (LEVANS), PLASMIDS CONTAINING THESE SEQUENCES AS WELL AS A PROCESS FOR PREPARING TRANSGENIC PLANTS)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 p35-CW-LEV 플라스미드의 구조를 나타내다. 이는 세 개의 단편 A, B 및 C를 포함한다. 단편 A는 꽃양배추 모자이크 바이러스(CaMV)의 35s 프로모터를 함유한다(뉴클레오티드 6906-7437). 단편 B는 에르위니아 아밀로보라로부터 얻은 레반 슈크라아제의 서열(뉴클레오티드 689-2122)을 함유한다(서열 번호 1). 단편 C는 Ti-플라스미드, pTi ACH 5의 유전자 3의 폴리아데닐화 신호를 함유한다.(뉴글레오티드 11749-11939).
제2도는 p35s-CY-LEV 플라스미드의 구조를 나타낸다. 이는 세 개의 단편 A, B 및 C를 포함한다. 단편 A는 꽃양배추 모자이크 바이러스(CaMV)의 35s 프로모터를 함유한다(뉴클레오티드 6906-7437). 단편 B는 에르위니아 아밀로보라로부터 얻은 레반 슈크라아제의 서열(뉴클레오티드 864-2122)을 함유한다(서얼 번호 1). 단편 C는 Ti-플라스미드, pTi ACH 5의 유전자 3의 폴리아데닐화 신호를 함유한다.
제3도는 p33-CW-LEV 플라스미드의 구조를 나타낸다. 이는 세 개의 단편 A, B 및 C를 포함한다. 단편 A는 파타틴 유전자 B33의 프로모터 영역의 DraI-DraI-단편(위치-1512에서 위치+14)을 함유한다. 단편 B는 에르위니아 아밀로보라로 부터 얻은 레반 슈크라아제의 서열(뉴클레오티드 689-2122)을 함유한다(서열 번호 1). 단편 C는 Ti-플라스미드, pTi ACH 5, T-DNA의 유전자 3의 폴리아데닐화 신호(뉴클레오티드 l1749-l1939)를 함유한다.

Claims (12)

  1. 레반 슈크라아제의 서열이 하기의 뉴클레오티드 서열(서열 번호 1)을 가지며, 에르위니아 아밀로보라(Erwinia amylovora)종의 그램-음성 박테리아로부터 유래하는, 식물 게놈 중에 통합됨으로써 잎 및 괴경에서 폴리프럭탄(레반)을 발현시킬 수 있는 것을 특징으로 하는, 레반 슈크라아제의 서열이 있는 DNA 서열.
  2. 잎 및 괴경에서 플리프럭탄(레반)을 발현하는 식물의 제조를 위한, 제1항에 따른 DNA 서열을 포함하는 플라스미드.
  3. 플라스미드 p35s-CW-LEV(DSM 7186).
  4. 플라스미드 p35s-CY-LEV(DSM 7187).
  5. 플라스미드 p33-CW-LEV(DSM 7188).
  6. (a) (i) 식물 내에서 활성이고 의도된 표적 조직 또는 표적 세포 내에서 RNA를 생성하는 프로모터, (ii) 제1항에 따른 레반 슈크라아제의 DNA서열, 및 (iii) 식물 세포 내에서 전사 종결 뿐만 아니라 RNA의 3′-말단에 폴리 A잔기틀 부착시키는 3′-비-번역 서열의 부분적 서열들을 갖는 DNA 서열을 제조하는 단게, (b) 제2항 내지 5항 중 어느 한 항에 따른 플라스미드를 사용하여 식물 세포로부터 얻은 재조합 이중 가닥 DNA분자의 식물 게놈 내에 상기 DNA 서열을 전달 및 통합하는 단계, 및 (c) 형질전환된 식물 세로로부터 온전한 식물 전체를 재생하는 단계로 이루어진, 잎 및 괴경에서 폴리프럭탄(레반)을 발현하는 유전자전환 식물의 제조 방법.
  7. 제6항에 있어서, 잎 및 괴경에서의 폴리프럭판(레반)슈크라아제 발현이 엽록체, 백색체, 미토콘드리아, 세포질, 세포간 공극 및(또는) 액포 네에서 폴리프럭탄(레반)슈크라아제 활성이 나타나도록 하는 것을 특징으로 하는 방법.
  8. 제6항 또는 7항에 따른 방법으로 얻은 식물.
  9. 제8항에 있어서, 농작물인 식물.
  10. 제8항 또는 9항에 있어서, 옥수수, 벼, 밀, 보리, 사탕무우, 사탕수수, 담배 또는 감자 식물인 식물.
  11. 잎 및 괴경에서 폴리프럭탄(레반)을 발현하는 식물의 제조를 위한, 제1항에 따른 DNA 서열의 용도.
  12. 잎 및 괴겨8에서 폴릭프럭탄(레반)을 발현하는 식물의 제조를 위한, 제2항 내지 5항 중 어느 한 항에 따른 플라스미드의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950700522A 1992-08-12 1993-08-09 폴리프럭탄(레반)을 생성하는 dna 서열, 이들 서열을 포함하는 플라스미드 및 유전자전환 식물의 제조 방법(dna sequences which lead to the formation of polyfructans (levans), plasmids containing these sequences as well as a process for preparing transgenic plants) KR950703059A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4227061A DE4227061A1 (de) 1992-08-12 1992-08-12 DNA-Sequenzen, die in der Pflanze die Bildung von Polyfructanen (Lävanen) hervorrufen, Plasmide enthaltend diese Sequenzen sowie Verfahren zur Herstellung transgener Pflanzen
DEP4227061.8 1992-08-12
PCT/EP1993/002110 WO1994004692A1 (en) 1992-08-12 1993-08-09 Dna sequences which lead to the formation of polyfructans (levans), plasmids containing these sequences as well as a process for preparing transgenic plants

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KR950703059A true KR950703059A (ko) 1995-08-23

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KR1019950700522A KR950703059A (ko) 1992-08-12 1993-08-09 폴리프럭탄(레반)을 생성하는 dna 서열, 이들 서열을 포함하는 플라스미드 및 유전자전환 식물의 제조 방법(dna sequences which lead to the formation of polyfructans (levans), plasmids containing these sequences as well as a process for preparing transgenic plants)

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US (3) US5792923A (ko)
EP (1) EP0663956B1 (ko)
JP (1) JP3643591B2 (ko)
KR (1) KR950703059A (ko)
AT (1) ATE297998T1 (ko)
AU (1) AU682472B2 (ko)
CA (1) CA2142308C (ko)
DE (2) DE4227061A1 (ko)
HU (1) HUT70977A (ko)
IL (1) IL106646A0 (ko)
UA (1) UA40598C2 (ko)
WO (1) WO1994004692A1 (ko)

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DE4227061A1 (de) 1994-02-17
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