TWI527901B - A single stranded nucleic acid molecule used to control gene expression - Google Patents

A single stranded nucleic acid molecule used to control gene expression Download PDF

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Publication number
TWI527901B
TWI527901B TW100124393A TW100124393A TWI527901B TW I527901 B TWI527901 B TW I527901B TW 100124393 A TW100124393 A TW 100124393A TW 100124393 A TW100124393 A TW 100124393A TW I527901 B TWI527901 B TW I527901B
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Taiwan
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aforementioned
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group
end side
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TW100124393A
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English (en)
Chinese (zh)
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TW201217523A (en
Inventor
Tadaaki Ohgi
Hirotake Hayashi
Hisao Shirohzu
Tomohiro Hamasaki
Akihiro Itoh
Hiroshi Suzuki
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Bonac Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pain & Pain Management (AREA)
  • Rheumatology (AREA)
  • Epidemiology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
TW100124393A 2010-07-08 2011-07-08 A single stranded nucleic acid molecule used to control gene expression TWI527901B (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP2010156122 2010-07-08
JP2010174915 2010-08-03
JP2010230808 2010-10-13
JP2010230806 2010-10-13
JP2010269824 2010-12-02
JP2010269823 2010-12-02

Publications (2)

Publication Number Publication Date
TW201217523A TW201217523A (en) 2012-05-01
TWI527901B true TWI527901B (zh) 2016-04-01

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
TW100124393A TWI527901B (zh) 2010-07-08 2011-07-08 A single stranded nucleic acid molecule used to control gene expression
TW104143857A TWI573870B (zh) 2010-07-08 2011-07-08 Single-stranded nucleic acid molecules used to control gene expression (I)

Family Applications After (1)

Application Number Title Priority Date Filing Date
TW104143857A TWI573870B (zh) 2010-07-08 2011-07-08 Single-stranded nucleic acid molecules used to control gene expression (I)

Country Status (17)

Country Link
EP (2) EP2431466B1 (enExample)
JP (3) JP4968811B2 (enExample)
KR (1) KR101849798B1 (enExample)
CN (2) CN102918156B (enExample)
AU (1) AU2011274854B2 (enExample)
BR (1) BR112013000481A2 (enExample)
CA (1) CA2804511C (enExample)
DK (2) DK2933333T3 (enExample)
ES (2) ES2550487T3 (enExample)
HU (2) HUE026520T2 (enExample)
MX (1) MX352568B (enExample)
NZ (2) NZ606232A (enExample)
PT (2) PT2431466E (enExample)
RU (1) RU2628311C2 (enExample)
SG (2) SG196779A1 (enExample)
TW (2) TWI527901B (enExample)
WO (1) WO2012005368A1 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691782B2 (en) 2010-08-03 2014-04-08 Bonac Corporation Single-stranded nucleic acid molecule having nitrogen-containing alicyclic skeleton
WO2013077446A1 (ja) * 2011-11-26 2013-05-30 株式会社ボナック 遺伝子発現制御のための一本鎖核酸分子
WO2013133393A1 (ja) * 2012-03-07 2013-09-12 学校法人東京医科大学 Ｖｅｇｆ遺伝子の発現抑制用一本鎖核酸分子
US20150105442A1 (en) * 2012-03-29 2015-04-16 Aqua Therapeutics Co., Ltd. Nucleic Acid Molecule Capable of Inhibiting Expression of Periostin Gene, method for Inhibiting Expression of Periostin Gene, and Use of Said Nucleic Acid Molecule
US9663784B2 (en) * 2012-05-26 2017-05-30 Bonac Corporation Single-stranded nucleic acid molecule for regulating expression of gene having delivering function
CN105899663B (zh) 2013-12-26 2019-07-26 学校法人东京医科大学 用于基因表达控制的人工模拟miRNA及其用途
CN106068324B (zh) 2013-12-27 2020-12-29 株式会社博纳克 控制基因表达的人工匹配型miRNA及其用途
HK1244035A1 (en) 2014-12-15 2018-07-27 Bonac Corporation Single-stranded nucleic acid molecule for inhibiting tgf- beta 1 expression
BR112017013664A2 (pt) 2014-12-27 2018-03-13 Bonac Corporation mirna de tipo natural, composição, métodos para inibir expressão de um gene alvo e para tratar uma doença, e, ácido nucleico de fita simples.
HK1244031A1 (en) 2015-03-27 2018-07-27 Bonac Corporation Single-chain nucleic acid molecule having delivery function and gene expression control ability
PL3369816T3 (pl) 2015-10-30 2024-07-22 Toray Industries, Inc. KOMPOZYCJA STABILNIE ZAWIERAJĄCA CZĄSTECZKĘ JEDNONICIOWEGO KWASU NUKLEINOWEGO, KTÓRA HAMUJE EKSPRESJĘ GENU TGF-β1
JP6634095B2 (ja) 2015-12-29 2020-01-22 国立大学法人北海道大学 プロレニン遺伝子またはプロレニン受容体遺伝子の発現を抑制する一本鎖核酸分子およびその用途
US11518994B2 (en) 2016-01-30 2022-12-06 Bonac Corporation Artificial single guide RNA and use thereof
JPWO2018182008A1 (ja) 2017-03-31 2020-02-06 株式会社ボナック 遺伝子発現制御機能を有する環状型核酸分子
JPWO2018199338A1 (ja) * 2017-04-27 2020-03-12 国立大学法人広島大学 Ｂ型肝炎治療用核酸分子
SG11201913541QA (en) 2017-07-28 2020-02-27 Kyorin Pharmaceutical Co Ltd Therapeutic agent for fibrosis
IL273245B2 (en) * 2017-09-15 2025-03-01 Commw Scient Ind Res Org RNA molecules
WO2019074071A1 (ja) 2017-10-11 2019-04-18 日東電工株式会社 核酸分子発現の調節
US20210188895A1 (en) 2017-10-13 2021-06-24 Bonac Corporation Single-stranded nucleic acid molecule, and production method therefor
TWI830718B (zh) * 2018-02-09 2024-02-01 日商住友化學股份有限公司 核酸分子之製造方法
US20210115485A1 (en) 2018-03-30 2021-04-22 Sumitomo Chemical Company, Limited Method for producing single-strand rna
US11920131B2 (en) 2018-03-30 2024-03-05 Toray Industries, Inc. Method of producing hairpin single-stranded RNA molecule
JP7405086B2 (ja) * 2018-10-02 2023-12-26 東レ株式会社 ヘアピン型一本鎖ｒｎａ分子の製造方法
JP7241098B2 (ja) 2019-01-25 2023-03-16 杏林製薬株式会社 線維症治療剤
JP7640461B2 (ja) 2019-10-11 2025-03-05 住友化学株式会社 核酸オリゴマーの製造方法
US12391716B2 (en) 2020-01-29 2025-08-19 Sumitomo Chemical Company, Limited Process of preparing nucleic acid oligomer
CN115003682A (zh) 2020-01-29 2022-09-02 住友化学株式会社 核酸寡聚物的制造方法
JP7613130B2 (ja) 2020-03-25 2025-01-15 味の素株式会社 リガーゼ変異体
WO2021193954A1 (ja) 2020-03-27 2021-09-30 住友化学株式会社 核酸オリゴマーの製造方法
EP4180442A4 (en) 2020-07-09 2025-05-14 Sumitomo Chemical Company, Limited METHOD FOR PRODUCING A NUCLEIC ACID OLIGOMER
US20240051986A1 (en) 2020-09-24 2024-02-15 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomer
EP4410770A1 (en) 2021-09-28 2024-08-07 Sumitomo Chemical Company, Limited Production method for purified dichloroacetic acid
EP4488282A4 (en) 2022-03-23 2025-11-19 Sumitomo Chemical Co PROCESS FOR THE PRODUCTION OF NUCLEIC ACID OLIGOMERS
JP7201190B1 (ja) 2022-06-03 2023-01-10 株式会社セツロテック 遺伝子改変動物由来の動物繊維
JPWO2024089953A1 (enExample) 2022-10-27 2024-05-02

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3687808A (en) 1969-08-14 1972-08-29 Univ Leland Stanford Junior Synthetic polynucleotides
US5631148A (en) * 1994-04-22 1997-05-20 Chiron Corporation Ribozymes with product ejection by strand displacement
US6509323B1 (en) 1998-07-01 2003-01-21 California Institute Of Technology Linear cyclodextrin copolymers
FR2790757B1 (fr) * 1999-03-09 2003-08-01 Bioalliance Pharma Oligonucleotides contenant une sequence antisens stabilises par une structure secondaire et compositions pharmaceutiques les contenant.
TWI321054B (en) 2000-12-19 2010-03-01 California Inst Of Techn Compositions containing inclusion complexes
US20060111312A1 (en) * 2002-02-22 2006-05-25 The John Hopkins University Antigene locks and therapeutic uses thereof
PT1527176E (pt) * 2002-08-05 2007-04-30 Atugen Ag Novas formas de muléculas de arn de interferência
US20040058886A1 (en) * 2002-08-08 2004-03-25 Dharmacon, Inc. Short interfering RNAs having a hairpin structure containing a non-nucleotide loop
JPWO2005030960A1 (ja) * 2003-09-30 2006-12-07 アンジェスＭｇ株式会社 ステイプル型オリゴヌクレオチドおよびそれからなる医薬
WO2006088490A2 (en) * 2004-06-30 2006-08-24 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a non-phosphate backbone linkage
WO2006074108A2 (en) * 2004-12-30 2006-07-13 Hauser Todd M Compositions and methods for modulating gene expression using self-protected oligonucleotides
WO2007108621A1 (en) * 2006-03-17 2007-09-27 Bioneer Corporation Sirna for inhibiting nf-kb/rela expression
JP5296328B2 (ja) 2007-05-09 2013-09-25 独立行政法人理化学研究所 １本鎖環状ｒｎａおよびその製造方法
CN101889087B (zh) * 2007-11-29 2013-04-03 苏州瑞博生物技术有限公司 一种干扰靶基因表达的复合分子及其制备方法
WO2009076321A2 (en) * 2007-12-07 2009-06-18 Halo-Bio Rnai Therapeutics Inc. Compositions and methods for modulating gene expression using asymmetrically-active precursor polynucleotides
JP5540312B2 (ja) * 2008-02-15 2014-07-02 独立行政法人理化学研究所 環状１本鎖核酸複合体およびその製造方法
JP5187580B2 (ja) 2008-12-26 2013-04-24 丸紅建材リース株式会社 掘削機の泥土飛散防止装置
JP2010174915A (ja) 2009-01-27 2010-08-12 Toyota Motor Corp 高圧ガスタンク
JP5233790B2 (ja) 2009-03-26 2013-07-10 船井電機株式会社 液晶モジュール
JP5449815B2 (ja) 2009-03-26 2014-03-19 住友化学株式会社 偏光板の製造方法
JP4885255B2 (ja) 2009-05-21 2012-02-29 株式会社エフピコ 包装用容器
JP2010269823A (ja) 2009-05-21 2010-12-02 British American Tobacco Japan Kk 喫煙品用容器

Also Published As

Publication number Publication date
DK2933333T3 (en) 2018-04-30
CA2804511C (en) 2021-07-06
EP2933333B1 (en) 2018-02-21
EP2431466A4 (en) 2012-04-25
NZ606232A (en) 2015-06-26
BR112013000481A2 (pt) 2019-10-15
ES2550487T3 (es) 2015-11-10
CA2804511A1 (en) 2012-01-12
JP2012130342A (ja) 2012-07-12
HK1181423A1 (zh) 2013-11-08
WO2012005368A1 (ja) 2012-01-12
KR20130090792A (ko) 2013-08-14
MX352568B (es) 2017-11-29
CN104818276A (zh) 2015-08-05
TWI573870B (zh) 2017-03-11
SG196779A1 (en) 2014-02-13
PT2431466E (pt) 2016-01-08
EP2431466B1 (en) 2015-10-07
JP6162182B2 (ja) 2017-07-12
PT2933333T (pt) 2018-04-24
JP5804642B2 (ja) 2015-11-04
SG186899A1 (en) 2013-02-28
TW201217523A (en) 2012-05-01
HUE026520T2 (en) 2016-06-28
TW201627500A (zh) 2016-08-01
DK2431466T3 (en) 2016-01-11
NZ704812A (en) 2017-01-27
RU2628311C2 (ru) 2017-08-15
EP2431466A1 (en) 2012-03-21
CN104818276B (zh) 2020-12-11
MX2013000315A (es) 2013-05-01
CN102918156A (zh) 2013-02-06
CN102918156B (zh) 2015-11-25
HUE037500T2 (hu) 2018-08-28
JP4968811B2 (ja) 2012-07-04
RU2013105329A (ru) 2014-08-20
JPWO2012005368A1 (ja) 2013-09-05
KR101849798B1 (ko) 2018-04-17
AU2011274854B2 (en) 2015-04-16
JP2015231381A (ja) 2015-12-24
EP2933333A1 (en) 2015-10-21
ES2670017T3 (es) 2018-05-29
AU2011274854A1 (en) 2013-02-14

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