RU2628311C2 - Молекула одноцепочечной нуклеиновой кислоты для контроля экспрессии генов - Google Patents

Молекула одноцепочечной нуклеиновой кислоты для контроля экспрессии генов Download PDF

Info

Publication number
RU2628311C2
RU2628311C2 RU2013105329A RU2013105329A RU2628311C2 RU 2628311 C2 RU2628311 C2 RU 2628311C2 RU 2013105329 A RU2013105329 A RU 2013105329A RU 2013105329 A RU2013105329 A RU 2013105329A RU 2628311 C2 RU2628311 C2 RU 2628311C2
Authority
RU
Russia
Prior art keywords
nucleic acid
bases
acid molecule
expression
stranded nucleic
Prior art date
Application number
RU2013105329A
Other languages
English (en)
Russian (ru)
Other versions
RU2013105329A (ru
Inventor
Тадааки ОХГИ
Хиротаке ХАЯСИ
Хисао СИРОХЗУ
Томохиро ХАМАСАКИ
Акихиро ИТОХ
Хироси Сузуки
Original Assignee
Бонак Корпорейшн
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Бонак Корпорейшн filed Critical Бонак Корпорейшн
Publication of RU2013105329A publication Critical patent/RU2013105329A/ru
Application granted granted Critical
Publication of RU2628311C2 publication Critical patent/RU2628311C2/ru

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7115—Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/712—Nucleic acids or oligonucleotides having modified sugars, i.e. other than ribose or 2'-deoxyribose
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7125—Nucleic acids or oligonucleotides having modified internucleoside linkage, i.e. other than 3'-5' phosphodiesters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pain & Pain Management (AREA)
  • Rheumatology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
RU2013105329A 2010-07-08 2011-07-08 Молекула одноцепочечной нуклеиновой кислоты для контроля экспрессии генов RU2628311C2 (ru)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
JP2010-156122 2010-07-08
JP2010156122 2010-07-08
JP2010-174915 2010-08-03
JP2010174915 2010-08-03
JP2010230806 2010-10-13
JP2010230808 2010-10-13
JP2010-230808 2010-10-13
JP2010-230806 2010-10-13
JP2010269824 2010-12-02
JP2010-269823 2010-12-02
JP2010269823 2010-12-02
JP2010-269824 2010-12-02
PCT/JP2011/065737 WO2012005368A1 (ja) 2010-07-08 2011-07-08 遺伝子発現制御のための一本鎖核酸分子

Publications (2)

Publication Number Publication Date
RU2013105329A RU2013105329A (ru) 2014-08-20
RU2628311C2 true RU2628311C2 (ru) 2017-08-15

Family

ID=45441339

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2013105329A RU2628311C2 (ru) 2010-07-08 2011-07-08 Молекула одноцепочечной нуклеиновой кислоты для контроля экспрессии генов

Country Status (18)

Country Link
US (3) US8785121B2 (enExample)
EP (2) EP2431466B1 (enExample)
JP (3) JP4968811B2 (enExample)
KR (1) KR101849798B1 (enExample)
CN (2) CN104818276B (enExample)
AU (1) AU2011274854B2 (enExample)
BR (1) BR112013000481A2 (enExample)
CA (1) CA2804511C (enExample)
DK (2) DK2933333T3 (enExample)
ES (2) ES2670017T3 (enExample)
HU (2) HUE026520T2 (enExample)
MX (1) MX352568B (enExample)
NZ (2) NZ704812A (enExample)
PT (2) PT2431466E (enExample)
RU (1) RU2628311C2 (enExample)
SG (2) SG186899A1 (enExample)
TW (2) TWI527901B (enExample)
WO (1) WO2012005368A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11920131B2 (en) 2018-03-30 2024-03-05 Toray Industries, Inc. Method of producing hairpin single-stranded RNA molecule

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG186899A1 (en) * 2010-07-08 2013-02-28 Bonac Corp Single-strand nucleic acid molecule for controlling gene expression
ES2533129T3 (es) 2010-08-03 2015-04-07 Bonac Corporation Molécula de ARN monocatenario que tiene esqueleto alicíclico que contiene nitrógeno
WO2013077446A1 (ja) * 2011-11-26 2013-05-30 株式会社ボナック 遺伝子発現制御のための一本鎖核酸分子
WO2013133393A1 (ja) * 2012-03-07 2013-09-12 学校法人東京医科大学 Ｖｅｇｆ遺伝子の発現抑制用一本鎖核酸分子
JP6283859B2 (ja) * 2012-03-29 2018-02-28 国立大学法人九州大学 ペリオスチン遺伝子の発現抑制核酸分子、ペリオスチン遺伝子の発現抑制方法、およびその用途
US9663784B2 (en) 2012-05-26 2017-05-30 Bonac Corporation Single-stranded nucleic acid molecule for regulating expression of gene having delivering function
WO2015099122A1 (ja) 2013-12-26 2015-07-02 学校法人東京医科大学 遺伝子発現制御のための人工ミミックｍｉＲＮＡおよびその用途
SG10201805087VA (en) 2013-12-27 2018-07-30 Bonac Corp Artificial match-type mirna for controlling gene expression and use therefor
HK1244035A1 (en) * 2014-12-15 2018-07-27 Bonac Corporation Single-stranded nucleic acid molecule for inhibiting tgf- beta 1 expression
WO2016104775A1 (ja) 2014-12-27 2016-06-30 株式会社ボナック 遺伝子発現制御のための天然型ｍｉＲＮＡおよびその用途
JP6602847B2 (ja) 2015-03-27 2019-11-06 株式会社ボナック デリバリー機能と遺伝子発現制御能を有する一本鎖核酸分子
EP3369816B1 (en) 2015-10-30 2024-03-13 Toray Industries, Inc. Composition stably containing single-stranded nucleic acid molecule that suppresses expression of tgf-beta1 gene
JP6634095B2 (ja) 2015-12-29 2020-01-22 国立大学法人北海道大学 プロレニン遺伝子またはプロレニン受容体遺伝子の発現を抑制する一本鎖核酸分子およびその用途
EP3409779A4 (en) 2016-01-26 2019-07-03 Nissan Chemical Corporation Single-stranded oligonucleotide
WO2017131237A1 (ja) 2016-01-30 2017-08-03 株式会社ボナック 人工単一ガイドｒｎａ及びその用途
CN108342386B (zh) * 2017-01-22 2022-04-15 广州市锐博生物科技有限公司 一种多聚寡核酸分子及其在多靶标干扰中的应用
JP7188087B2 (ja) 2017-02-06 2022-12-13 日産化学株式会社 一本鎖オリゴヌクレオチド
US20200038427A1 (en) 2017-03-31 2020-02-06 Bonac Corporation Cyclic nucleic acid molecule having gene expression control function
WO2018199338A1 (ja) * 2017-04-27 2018-11-01 国立大学法人広島大学 Ｂ型肝炎治療用核酸分子
WO2019022257A1 (ja) 2017-07-28 2019-01-31 杏林製薬株式会社 線維症治療剤
CN119506283A (zh) * 2017-09-15 2025-02-25 联邦科学技术研究组织 Rna分子
JP7208911B2 (ja) 2017-10-11 2023-01-19 日東電工株式会社 核酸分子発現の調節
WO2019074110A1 (ja) 2017-10-13 2019-04-18 株式会社ボナック 一本鎖核酸分子およびその製造方法
TWI830718B (zh) * 2018-02-09 2024-02-01 日商住友化學股份有限公司 核酸分子之製造方法
US20210115485A1 (en) 2018-03-30 2021-04-22 Sumitomo Chemical Company, Limited Method for producing single-strand rna
CN112673102A (zh) * 2018-10-02 2021-04-16 东丽株式会社 发夹型单链rna分子的制备方法
WO2020152869A1 (ja) 2019-01-25 2020-07-30 杏林製薬株式会社 線維症治療剤
EP4726043A3 (en) 2019-02-18 2026-06-24 Ajinomoto Co., Inc. Method for producing modified oligonucleotide including complementary sequence
CN114502566B (zh) 2019-10-11 2025-02-11 住友化学株式会社 核酸寡聚物的制造方法
CN115003680B (zh) 2020-01-29 2025-01-28 住友化学株式会社 制备核酸寡聚物的方法
KR20220133878A (ko) 2020-01-29 2022-10-05 스미또모 가가꾸 가부시끼가이샤 핵산 올리고머의 제조 방법
JP7613130B2 (ja) * 2020-03-25 2025-01-15 味の素株式会社 リガーゼ変異体
CN115335387A (zh) 2020-03-27 2022-11-11 住友化学株式会社 核酸寡聚物的制造方法
KR20230034969A (ko) 2020-07-09 2023-03-10 스미또모 가가꾸 가부시끼가이샤 핵산 올리고머의 제조 방법
KR20230074205A (ko) 2020-09-24 2023-05-26 스미또모 가가꾸 가부시끼가이샤 핵산 올리고머의 제조 방법
EP4410770A4 (en) 2021-09-28 2026-03-25 Sumitomo Chemical Co PROCESS FOR THE PRODUCTION OF PURIFIED DICHLOROACETIC ACID
EP4488282A4 (en) 2022-03-23 2025-11-19 Sumitomo Chemical Co PROCESS FOR THE PRODUCTION OF NUCLEIC ACID OLIGOMERS
JP7201190B1 (ja) 2022-06-03 2023-01-10 株式会社セツロテック 遺伝子改変動物由来の動物繊維
JP7825064B2 (ja) 2022-10-27 2026-03-05 住友化学株式会社 オリゴヌクレオチドの製造方法
WO2025229953A1 (ja) 2024-04-30 2025-11-06 住友化学株式会社 オリゴヌクレオチドの製造方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007108621A1 (en) * 2006-03-17 2007-09-27 Bioneer Corporation Sirna for inhibiting nf-kb/rela expression

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3687808A (en) 1969-08-14 1972-08-29 Univ Leland Stanford Junior Synthetic polynucleotides
US5631148A (en) * 1994-04-22 1997-05-20 Chiron Corporation Ribozymes with product ejection by strand displacement
GB9621367D0 (en) 1996-10-14 1996-12-04 Isis Innovation Chiral peptide nucleic acids
US6509323B1 (en) 1998-07-01 2003-01-21 California Institute Of Technology Linear cyclodextrin copolymers
FR2790757B1 (fr) 1999-03-09 2003-08-01 Bioalliance Pharma Oligonucleotides contenant une sequence antisens stabilises par une structure secondaire et compositions pharmaceutiques les contenant.
US6962906B2 (en) 2000-03-14 2005-11-08 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
TWI321054B (en) 2000-12-19 2010-03-01 California Inst Of Techn Compositions containing inclusion complexes
EP1627061B1 (en) 2001-05-18 2009-08-12 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING CHEMICALLY MODIFIED SHORT INTERFERING NUCLEIC ACID (siNA)
WO2003072745A2 (en) * 2002-02-22 2003-09-04 Eshleman James R Antigene locks and therapeutic uses thereof
WO2003079757A2 (en) 2002-03-20 2003-10-02 Massachusetts Institute Of Technology Hiv therapeutic
EP1857547B2 (en) * 2002-08-05 2020-12-02 Silence Therapeutics GmbH Further novel forms of interfering RNA molecules
AU2003256857A1 (en) * 2002-08-08 2004-02-25 Dharmacon, Inc. Short interfering rnas having a hairpin structure containing a non-nucleotide loop
JP2006522158A (ja) 2003-04-03 2006-09-28 アルナイラム ファーマシューティカルズ インコーポレイテッド ｉＲＮＡ複合体
US7595301B2 (en) 2003-09-30 2009-09-29 Anges Mg, Inc. Staple type oligonucleotide and drug comprising the same
JP2008504840A (ja) 2004-06-30 2008-02-21 アルニラム ファーマスーティカルズ インコーポレイテッド 非リン酸骨格結合を含むオリゴヌクレオチド
JP2008526213A (ja) * 2004-12-30 2008-07-24 トッド エム． ハウザー， 自己保護オリゴヌクレオチドを使用する、遺伝子発現を調節するための組成物および方法
ES2538217T3 (es) 2007-03-21 2015-06-18 Brookhaven Science Associates, Llc Composiciones combinadas antisentido de horquilla y métodos para modular la expresión
JP5296328B2 (ja) 2007-05-09 2013-09-25 独立行政法人理化学研究所 １本鎖環状ｒｎａおよびその製造方法
EP2171089B1 (en) 2007-06-25 2014-01-22 Universität Duisburg-Essen Screening-method for polymorphic markers in htra1 gene in neurodegenerative disorders
US20100317714A1 (en) * 2007-11-29 2010-12-16 Suzhou Ribo Life Science Co., Ltd Complex molecule interfering the expression of target genes and its preparing methods
EP2229186A2 (en) 2007-12-04 2010-09-22 Alnylam Pharmaceuticals Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US20110159586A1 (en) * 2007-12-07 2011-06-30 Halo-Bio Rnai Therapeutics, Inc. Compositions and methods for modulating gene expression using asymmetrically-active precursor polynucleotides
EP2256191A4 (en) * 2008-02-15 2011-07-06 Riken CYCLIC SINGLE NUCLEIC ACID COMPLEX AND METHOD FOR THE PRODUCTION THEREOF
JP5187580B2 (ja) 2008-12-26 2013-04-24 丸紅建材リース株式会社 掘削機の泥土飛散防止装置
JP2010174915A (ja) 2009-01-27 2010-08-12 Toyota Motor Corp 高圧ガスタンク
JP5449815B2 (ja) 2009-03-26 2014-03-19 住友化学株式会社 偏光板の製造方法
JP5233790B2 (ja) 2009-03-26 2013-07-10 船井電機株式会社 液晶モジュール
JP2010269823A (ja) 2009-05-21 2010-12-02 British American Tobacco Japan Kk 喫煙品用容器
JP4885255B2 (ja) 2009-05-21 2012-02-29 株式会社エフピコ 包装用容器
US20110052666A1 (en) 2009-09-03 2011-03-03 Medtronic, Inc. Compositions, Methods, and Systems for SIRNA Delivery
WO2011132672A1 (ja) 2010-04-19 2011-10-27 独立行政法人理化学研究所 機能性核酸の安定化法
SG186899A1 (en) * 2010-07-08 2013-02-28 Bonac Corp Single-strand nucleic acid molecule for controlling gene expression

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007108621A1 (en) * 2006-03-17 2007-09-27 Bioneer Corporation Sirna for inhibiting nf-kb/rela expression

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LEIRDAL M., et al., Gene silencing in mammalian cells by performed small RNAduplexes, Biochemical and biophysical research communication, 2001, No.295, pp.744-748. JIA Y., et al., Characterization of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from halotolerant alga Dunaliella salina and inhibition of its expression by RNAi, Curr Microbiol, 2009, 58, 426-431. *
РУСИН И.В., РНК-интерференция: микро-РНК как новые регуляторы работы генов и перспективные терапевтические мишени, Электронный сборник научных трудов "Здоровье и образование в XXI веке", 2009, No.6, том 11, с.268-269. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11920131B2 (en) 2018-03-30 2024-03-05 Toray Industries, Inc. Method of producing hairpin single-stranded RNA molecule

Also Published As

Publication number Publication date
WO2012005368A1 (ja) 2012-01-12
JP2012130342A (ja) 2012-07-12
US20120010271A1 (en) 2012-01-12
EP2431466A1 (en) 2012-03-21
CN104818276A (zh) 2015-08-05
US20150011605A1 (en) 2015-01-08
CA2804511A1 (en) 2012-01-12
JPWO2012005368A1 (ja) 2013-09-05
DK2933333T3 (en) 2018-04-30
NZ606232A (en) 2015-06-26
AU2011274854A1 (en) 2013-02-14
ES2550487T3 (es) 2015-11-10
TWI527901B (zh) 2016-04-01
CN104818276B (zh) 2020-12-11
JP4968811B2 (ja) 2012-07-04
MX2013000315A (es) 2013-05-01
HK1181423A1 (zh) 2013-11-08
SG186899A1 (en) 2013-02-28
KR20130090792A (ko) 2013-08-14
RU2013105329A (ru) 2014-08-20
BR112013000481A2 (pt) 2019-10-15
MX352568B (es) 2017-11-29
JP5804642B2 (ja) 2015-11-04
EP2933333B1 (en) 2018-02-21
AU2011274854B2 (en) 2015-04-16
DK2431466T3 (en) 2016-01-11
TW201627500A (zh) 2016-08-01
JP2015231381A (ja) 2015-12-24
SG196779A1 (en) 2014-02-13
US8785121B2 (en) 2014-07-22
CN102918156A (zh) 2013-02-06
TWI573870B (zh) 2017-03-11
PT2431466E (pt) 2016-01-08
CN102918156B (zh) 2015-11-25
EP2431466A4 (en) 2012-04-25
HUE026520T2 (en) 2016-06-28
KR101849798B1 (ko) 2018-04-17
CA2804511C (en) 2021-07-06
US20160108400A1 (en) 2016-04-21
EP2431466B1 (en) 2015-10-07
JP6162182B2 (ja) 2017-07-12
EP2933333A1 (en) 2015-10-21
HUE037500T2 (hu) 2018-08-28
PT2933333T (pt) 2018-04-24
TW201217523A (en) 2012-05-01
NZ704812A (en) 2017-01-27
ES2670017T3 (es) 2018-05-29

Similar Documents

Publication Publication Date Title
US9206422B2 (en) Single-stranded nucleic acid molecule having nitrogen-containing alicyclic skeleton
EP2933333B1 (en) Single-strand nucleic acid molecule for controlling gene expression
WO2013077446A1 (ja) 遺伝子発現制御のための一本鎖核酸分子
HK1189910B (en) Single-stranded rna molecule having nitrogen-containing alicyclic skeleton