SG10201710218RA - Sc-beta cells and compositions and methods for generating the same - Google Patents

Sc-beta cells and compositions and methods for generating the same

Info

Publication number
SG10201710218RA
SG10201710218RA SG10201710218RA SG10201710218RA SG10201710218RA SG 10201710218R A SG10201710218R A SG 10201710218RA SG 10201710218R A SG10201710218R A SG 10201710218RA SG 10201710218R A SG10201710218R A SG 10201710218RA SG 10201710218R A SG10201710218R A SG 10201710218RA
Authority
SG
Singapore
Prior art keywords
compositions
generating
methods
same
beta cells
Prior art date
Application number
SG10201710218RA
Other languages
English (en)
Inventor
Quinn P Peterson
Felicia J Pagliuca
Douglas A Melton
Jeffrey R Millman
Michael Saris Segel
Mads Gürtler
Alireza Rezania
Benjamin Fryer
Original Assignee
Harvard College
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Janssen Biotech Inc filed Critical Harvard College
Publication of SG10201710218RA publication Critical patent/SG10201710218RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/375—Thyroid stimulating hormone [TSH]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/395—Thyroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/22—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Diabetes (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Immunology (AREA)
  • Physiology (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Emergency Medicine (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
SG10201710218RA 2013-06-11 2014-06-11 Sc-beta cells and compositions and methods for generating the same SG10201710218RA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361833898P 2013-06-11 2013-06-11
US201461972212P 2014-03-28 2014-03-28

Publications (1)

Publication Number Publication Date
SG10201710218RA true SG10201710218RA (en) 2018-01-30

Family

ID=52022750

Family Applications (3)

Application Number Title Priority Date Filing Date
SG11201510176VA SG11201510176VA (en) 2013-06-11 2014-06-11 SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME
SG10201710218RA SG10201710218RA (en) 2013-06-11 2014-06-11 Sc-beta cells and compositions and methods for generating the same
SG11201510177WA SG11201510177WA (en) 2013-06-11 2014-06-11 SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201510176VA SG11201510176VA (en) 2013-06-11 2014-06-11 SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201510177WA SG11201510177WA (en) 2013-06-11 2014-06-11 SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME

Country Status (17)

Country Link
US (14) US20170029778A1 (pt)
EP (3) EP3008170A4 (pt)
JP (7) JP6514689B2 (pt)
KR (12) KR102205234B1 (pt)
CN (4) CN112226402A (pt)
AU (6) AU2014284677B2 (pt)
BR (2) BR112015030946A8 (pt)
CA (2) CA2915088A1 (pt)
DE (2) DE202014011287U1 (pt)
DK (1) DK3008168T3 (pt)
GB (3) GB2531952C (pt)
HK (1) HK1257187B (pt)
MX (2) MX2015017103A (pt)
PH (1) PH12015502767A1 (pt)
RU (2) RU2692595C2 (pt)
SG (3) SG11201510176VA (pt)
WO (2) WO2014201167A1 (pt)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101617243B1 (ko) 2007-07-31 2016-05-02 라이프스캔, 인코포레이티드 인간 배아 줄기 세포의 분화
MX2010005805A (es) 2007-11-27 2010-06-09 Lifescan Inc Diferenciacion de celulas madre embrionarias humanas.
SG10201608914WA (en) 2011-12-22 2016-12-29 Janssen Biotech Inc Differentiation of human embryonic stem cells into single hormonal insulin positive cells
KR101942769B1 (ko) 2012-12-31 2019-01-28 얀센 바이오테크 인코포레이티드 Hb9 조절제를 사용하는 인간 배아 줄기세포의 췌장 내분비 세포로의 분화
JP2016518357A (ja) 2013-04-08 2016-06-23 プレジデント アンド フェローズ オブ ハーバード カレッジ 骨格筋幹細胞を若返らせる方法および組成物
KR102205234B1 (ko) 2013-06-11 2021-01-20 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 SC-β 세포 및 조성물 그리고 그 생성 방법
CN117821369A (zh) 2014-05-16 2024-04-05 詹森生物科技公司 小分子增强胰腺内分泌细胞中的mafa表达的用途
DK3234110T3 (da) 2014-12-18 2024-05-13 Harvard College FREMGANGSMÅDER TIL GENERERING AF STAMCELLE-AFLEDTE ß-CELLER OG ANVENDELSER DERAF
CN107614678B (zh) * 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
WO2016100921A1 (en) * 2014-12-18 2016-06-23 President And Fellows Of Harvard College METHODS FOR GENERATING STEM CELL-DERIVED β CELLS AND USES THEREOF
US10443042B2 (en) 2014-12-18 2019-10-15 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
WO2016100857A1 (en) * 2014-12-19 2016-06-23 Regeneron Pharmaceuticals, Inc. Stem cells for modeling type 2 diabetes
WO2016172564A1 (en) * 2015-04-23 2016-10-27 The Regents Of The University Of California Controlled induction of human pancreatic progenitors produces functional beta-like cells
ES2840076T3 (es) 2015-04-24 2021-07-06 Univ Copenhagen Aislamiento de células progenitoras pancreáticas bona fide
WO2016170067A1 (en) * 2015-04-24 2016-10-27 University Of Copenhagen Method for production of insulin-producing cells
WO2017019702A1 (en) 2015-07-27 2017-02-02 The Regents Of The University Of California Methods and compositions for producing pancreatic beta cells
JP6862347B2 (ja) * 2015-10-29 2021-04-21 学校法人順天堂 膵内分泌細胞の製造方法、及び分化転換剤
US11116777B2 (en) 2015-11-10 2021-09-14 City Of Hope Conditioning regimens and methods for inducing mixed chimerism
WO2017116569A1 (en) * 2015-12-31 2017-07-06 City Of Hope Methods for treating diabetes
JP6948331B2 (ja) 2016-01-06 2021-10-13 プレジデント アンド フェローズ オブ ハーバード カレッジ Ｇｄｆ１１での処置は、体重増加を防止し、耐糖能を改善し、そして脂肪肝を低減する
US11473061B2 (en) 2016-02-01 2022-10-18 Cedars-Sinai Medical Center Systems and methods for growth of intestinal cells in microfluidic devices
CN108699515A (zh) * 2016-02-24 2018-10-23 诺和诺德股份有限公司 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞
US10201540B2 (en) 2016-03-02 2019-02-12 Frequency Therapeutics, Inc. Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using GSK3 inhibitors: I
EP3423069A4 (en) * 2016-03-02 2020-04-08 Frequency Therapeutics, Inc. METHOD FOR CONTROLLED PROLIFERATION OF VESTIBULARY STEM CELLS / GENERATION OF INNER EAR HAIR CELLS USING WNT AND TGF-BETA INHIBITION
US10213511B2 (en) 2016-03-02 2019-02-26 Frequency Therapeutics, Inc. Thermoreversible compositions for administration of therapeutic agents
MA45329A (fr) 2016-04-08 2019-02-13 Univ California Production de cellules bêta matures pleinement fonctionnelles à partir de progénitrices pancréatiques humaines
US20190151371A1 (en) * 2016-06-09 2019-05-23 Georgetown University Process for continuous cell culture of islet cells
MA45502A (fr) * 2016-06-21 2019-04-24 Janssen Biotech Inc Génération de cellules bêta fonctionnelles dérivées de cellules souches pluripotentes humaines ayant une respiration mitochondriale glucose-dépendante et une réponse en sécrétion d'insuline en deux phases
CN109963938B (zh) * 2016-11-16 2024-04-19 美国绿阳生物技术及医药公司 以RNA通过干细胞分化诱导胰脏β细胞
US11913022B2 (en) 2017-01-25 2024-02-27 Cedars-Sinai Medical Center In vitro induction of mammary-like differentiation from human pluripotent stem cells
KR101745297B1 (ko) * 2017-02-02 2017-06-09 경북대학교 산학협력단 비만 진단용 조성물 및 이의 용도
US11767513B2 (en) 2017-03-14 2023-09-26 Cedars-Sinai Medical Center Neuromuscular junction
US11414648B2 (en) 2017-03-24 2022-08-16 Cedars-Sinai Medical Center Methods and compositions for production of fallopian tube epithelium
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
CA3066055A1 (en) 2017-06-14 2018-12-20 Semma Therapeutics, Inc. Devices and methods for delivering therapeutics
US20210355448A1 (en) * 2017-06-26 2021-11-18 City Of Hope Methods of islet cell culture
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
CN111246864A (zh) * 2017-07-21 2020-06-05 森玛治疗公司 干细胞衍生的胰腺β细胞的重聚
CA2983845C (en) 2017-10-26 2024-01-30 University Of Copenhagen Generation of glucose-responsive beta cells
IL274436B2 (en) * 2017-11-15 2024-01-01 Semma Therapeutics Inc Preparations for the production of islet cells and methods of use
EP3758719A4 (en) * 2018-03-02 2021-12-08 Vertex Pharmaceuticals Inc. METHODS FOR IMPROVING THE DIFFERENTIATION OF STEM CELLS INTO BETA CELLS
US20210000880A1 (en) * 2018-03-23 2021-01-07 Cedars-Sinai Medical Center Methods of use of islet cells
US11981918B2 (en) 2018-04-06 2024-05-14 Cedars-Sinai Medical Center Differentiation technique to generate dopaminergic neurons from induced pluripotent stem cells
WO2019217875A1 (en) * 2018-05-11 2019-11-14 Cornell University Method of differentiation of human pluripotent stem cells to monohormonal cells
EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated ISLE DIFFERENTIATION DERIVED FROM STEM CELLS
WO2020037323A1 (en) 2018-08-17 2020-02-20 Frequency Therapeutics, Inc. Compositions and methods for generating hair cells by upregulating jag-1
WO2020037326A1 (en) 2018-08-17 2020-02-20 Frequency Therapeutics, Inc. Compositions and methods for generating hair cells by downregulating foxo
WO2020043292A1 (en) * 2018-08-30 2020-03-05 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
US10724052B2 (en) 2018-09-07 2020-07-28 Crispr Therapeutics Ag Universal donor cells
CN113056289A (zh) 2018-09-21 2021-06-29 哈佛学院校长同事会 用于治疗糖尿病的方法和组合物以及用于富集编码分泌蛋白的mRNA的方法
WO2020069515A1 (en) * 2018-09-28 2020-04-02 Memorial Sloan-Kettering Cancer Center Stem cell-derived human microglial cells, methods of making and methods of use
CN112888778A (zh) * 2018-10-15 2021-06-01 Cynity株式会社 通过低分子化合物由源自内胚层组织或器官的细胞制备干细胞/祖细胞的方法
KR102115960B1 (ko) * 2018-11-28 2020-05-27 송미연 인간 중간엽 줄기세포를 갑상선 호르몬 분비세포로 분화 방법
WO2020206046A1 (en) 2019-04-01 2020-10-08 The Broad Institute, Inc. Methods and compositions for cell therapy
JP2022528917A (ja) * 2019-04-08 2022-06-16 ノヴォ ノルディスク アー／エス 幹細胞由来の胚体内胚葉からの膵臓内胚葉の生成
KR102167257B1 (ko) * 2019-05-28 2020-10-19 부산대학교 산학협력단 토마티딘 처리를 통한 줄기세포의 성숙화된 심근세포로의 분화유도 촉진 방법
AU2020284245B2 (en) 2019-05-31 2023-10-05 Viacyte, Inc. A biocompatible membrane composite
AU2020282355B2 (en) 2019-05-31 2023-11-02 Viacyte, Inc. A biocompatible membrane composite
CA3139292A1 (en) 2019-05-31 2020-12-03 Timothy M. BRUHN Cell encapsulation devices with controlled oxygen diffusion distances
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
WO2020247954A1 (en) * 2019-06-06 2020-12-10 President And Fellows Of Harvard College Compositions and methods for maturing stem cell-derived beta cells
CA3144948A1 (en) 2019-06-25 2020-12-30 Vertex Pharmaceuticals Incorporated Enhanced differentiation of beta cells
WO2021030424A1 (en) * 2019-08-13 2021-02-18 Semma Therapeutics, Inc. Pancreatic differentiation
US10740609B1 (en) * 2019-08-30 2020-08-11 Numerica Corporation System and method for space object detection in daytime sky images
WO2021044379A1 (en) 2019-09-05 2021-03-11 Crispr Therapeutics Ag Universal donor cells
US11116797B2 (en) 2019-09-05 2021-09-14 Crispr Therapeutics Ag Universal donor cells
AU2020361709A1 (en) * 2019-10-10 2022-05-26 The Regents Of The University Of Colorado, A Body Corporate Use of ENTPD3 for identification, isolation, and enhancing mature stem cell derived insulin-producing cells
US20230014430A1 (en) * 2019-11-20 2023-01-19 Washington University Methods and Compositions for Generating Functionally Mature Beta Cells and Uses Thereof
KR20220143063A (ko) 2020-02-19 2022-10-24 남미 테라퓨틱스, 인크. 암의 치료에 유용한 TGFβ 길항제 전구약물을 함유하는 제제화된 및/또는 공동-제제화된 리포솜 조성물 및 그의 방법
CN111269875B (zh) * 2020-03-24 2022-04-08 山东兴瑞生物科技有限公司 一种利用自体免疫细胞定向分化为胰岛细胞的方法
WO2021212044A1 (en) * 2020-04-16 2021-10-21 Washington University Methods and compositions for improving sc-beta cells or enhancing their utility
US20220010282A1 (en) * 2020-06-16 2022-01-13 The Broad Institute, Inc. Three-dimensional cell culture, devices, and use thereof
AU2021315814A1 (en) * 2020-07-31 2023-03-02 Vertex Pharmaceuticals Incorporated In vitro differentiation of pancreatic endocrine cells
JP2024503291A (ja) 2020-12-31 2024-01-25 クリスパー セラピューティクス アクチェンゲゼルシャフト ユニバーサルドナー細胞
CN112980771B (zh) * 2021-03-05 2023-12-19 贝康医学科技有限公司 一种制备胰腺beta细胞的方法及其应用
CN113046299A (zh) * 2021-03-19 2021-06-29 上海爱萨尔生物科技有限公司 一种诱导多能干细胞定向分化制备胰岛β细胞的添加剂
CN112961823B (zh) * 2021-03-19 2024-01-23 上海爱萨尔生物科技有限公司 一种诱导多能干细胞定向分化制备胰岛β细胞的培养液
CN112980774B (zh) * 2021-03-19 2022-04-01 上海爱萨尔生物科技有限公司 一种诱导多能干细胞定向分化制备胰岛β细胞的培养方法
CN113234664B (zh) * 2021-05-11 2024-05-10 澳门大学 一种胰腺祖细胞的制备方法及其应用
US20220403340A1 (en) * 2021-06-11 2022-12-22 Washington University Media for cryopreserved cells and methods of making and using same
US20220409855A1 (en) 2021-06-29 2022-12-29 Staffan Holmin Methods of delivering cells and therapeutic agents to organs and extravascular sites
KR20240057433A (ko) 2021-09-21 2024-05-02 각코호진 오키나와가가쿠기쥬츠다이가쿠인 다이가쿠가쿠엔 인간 뇌 오가노이드를 이용한 시누클레인병증에 대한 약물 시험용 플랫폼
CA3234231A1 (en) 2021-10-21 2023-04-27 Vertex Pharmaceuticals Incorporated Hypoimmune cells
WO2023072170A1 (en) * 2021-10-28 2023-05-04 Sunshine Lake Pharma Co., Ltd. A METHOD FOR PROMOTING THE DIFFERENTIATION OF STEM CELLS INTO FUNCTIONALLY MATURE ISLET β CELLS AND USE THEREOF
US11992506B2 (en) 2021-11-01 2024-05-28 Vertex Pharmaceuticals Incorporated Stem cell derived pancreatic islet differentiation
WO2023097513A1 (en) * 2021-11-30 2023-06-08 Hangzhou Reprogenix Bioscience, Inc. Method of generating functional islets from pluripotent stem cells
WO2023150493A2 (en) * 2022-02-01 2023-08-10 Vertex Pharmaceuticals Incorporated Cell therapy for diabetes
US20230330264A1 (en) 2022-04-15 2023-10-19 Smartcella Solutions Ab COMPOSITIONS AND METHODS FOR EXOSOME-MEDIATED DELIVERY OF mRNA AGENTS
WO2023235568A1 (en) * 2022-06-03 2023-12-07 Cornell University Derivation of glucose-responsive insulin-secreting cells and organoids from human stomach cells and their use to treat diabetes
WO2024091676A1 (en) * 2022-10-28 2024-05-02 The Regents Of The University Of Colorado, A Body Corporate Compositions and methods for accelerated production of thymic cells from pluripotent stem cells
CN117672411B (zh) * 2023-12-05 2024-05-14 首都医科大学附属北京世纪坛医院 一种中药成分的Hormesis效应研究方法及中药成分小檗碱的应用

Family Cites Families (166)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US130A (en) 1837-02-16 Improvement in metallic solutions for the preservation of timber
US6774A (en) 1849-10-09 Improved jointed center-board
US3270960A (en) 1964-09-11 1966-09-06 Sperry Rand Corp Fluid sensor
DE1570610C3 (de) 1965-10-01 1974-08-01 Bayer Ag, 5090 Leverkusen Verfahren zur Herstellung von Poly-N-oxyden
US3773919A (en) 1969-10-23 1973-11-20 Du Pont Polylactide-drug mixtures
US4391909A (en) 1979-03-28 1983-07-05 Damon Corporation Microcapsules containing viable tissue cells
US4378016A (en) 1981-07-15 1983-03-29 Biotek, Inc. Artificial endocrine gland containing hormone-producing cells
US5425764A (en) 1992-07-30 1995-06-20 The University Of Toledo Bioartificial pancreas
US6469017B1 (en) 1998-01-16 2002-10-22 Cell Therapeutics, Inc. Method of inhibiting interleukin-12 signaling
US7018419B2 (en) 1994-05-20 2006-03-28 Spielberg Theodore E Microporous encapsulated endocrine cell disks, biconcave disks and multidimpled chambers for hormonal replacement
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
WO1996031242A1 (en) 1995-04-07 1996-10-10 Albert Einstein College Of Medicine Of Yeshiva University RECOMBINANT β-CELL AND USES THEREOF
US6187991B1 (en) 1995-05-23 2001-02-13 Pfizer Inc Transgenic animal models for type II diabetes mellitus
AU6496996A (en) 1996-02-23 1997-09-10 Circle Biomedical, Inc. Novel artificial pancreas
US5994619A (en) 1996-04-01 1999-11-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of chimeric bovine or porcine animals using cultured inner cell mass cells
KR20000048820A (ko) 1996-10-01 2000-07-25 게론 코포레이션 텔로머라제 역전사 효소
AU5734998A (en) 1997-01-10 1998-08-03 Life Technologies, Inc. Embryonic stem cell serum replacement
US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
AU729377B2 (en) 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
CA2397292A1 (en) 2000-01-14 2001-07-19 Bresagen Limited Cell production
GB0005251D0 (en) 2000-03-03 2000-04-26 Merck Sharp & Dohme Therapeutic compounds
US6436704B1 (en) 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
JP5943533B2 (ja) 2000-05-17 2016-07-06 アステリアス バイオセラピューティクス インコーポレイテッド 神経前駆細胞の集団
US7094874B2 (en) 2000-05-26 2006-08-22 Bristol-Myers Squibb Co. Soluble CTLA4 mutant molecules
US6906810B2 (en) 2000-07-13 2005-06-14 Morgan Guaranty Trust Company Method and system for allowing an on-line user to selectively customize a document for printing in a single batch process
US7163918B2 (en) 2000-08-22 2007-01-16 New River Pharmaceuticals Inc. Iodothyronine compositions
US6916640B2 (en) 2000-10-12 2005-07-12 Agency For Science, Technology And Research Multi-layer cell encapsulation for tissue engineering
WO2002030462A2 (en) 2000-10-13 2002-04-18 Curis, Inc. Hedgehog antagonists, methods and uses related thereto
US6576464B2 (en) 2000-11-27 2003-06-10 Geron Corporation Methods for providing differentiated stem cells
US20040121460A1 (en) 2001-01-24 2004-06-24 Lumelsky Nadya L Differentiation of stem cells to pancreatic endocrine cells
JP2004531262A (ja) 2001-05-15 2004-10-14 ラッパポート ファミリー インスチチュート フォア リサーチ イン ザ メディカル サイエンシズ ヒト胚性幹細胞由来インスリン産生細胞
CA2456981C (en) 2001-08-06 2012-02-28 Bresagen, Inc. Alternative compositions and methods for the culture of stem cells
US20030054331A1 (en) 2001-09-14 2003-03-20 Stemsource, Inc. Preservation of non embryonic cells from non hematopoietic tissues
JP2005506074A (ja) 2001-10-18 2005-03-03 イクシオン・バイオテクノロジー・インコーポレーテッド 肝臓の幹細胞および前駆細胞の膵臓機能細胞への転換
US20030077256A1 (en) 2001-10-19 2003-04-24 Paul Czernichow Pancreas regeneration using embryonic pancreatic cells
US7157278B2 (en) 2001-12-04 2007-01-02 Organogenesis, Inc. Cultured cells from pancreatic islets
EP1463798A4 (en) 2001-12-07 2005-01-19 Geron Corp ISLAND CELLS FROM HUMAN EMBRYONAL STEM CELLS
EP2292734A1 (en) 2001-12-07 2011-03-09 Geron Corporation Hematopoietic cells from human embryonic stem cells
EP2305276A3 (en) 2001-12-07 2011-09-21 Cytori Therapeutics, Inc. Processed lipoaspirate cells for use in therapy
US20060003446A1 (en) 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
WO2003102171A1 (en) 2002-05-28 2003-12-11 Becton, Dickinson And Company Expansion and transdifferentiation of human acinar cells
JP2006506047A (ja) * 2002-05-28 2006-02-23 ノボセル インコーポレイテッド インスリン産生細胞のための方法、成分並びに成長および分化因子
WO2004050827A2 (en) 2002-12-05 2004-06-17 Technion Research & Development Foundation Ltd. Cultured human pancreatic islets, and uses thereof
WO2004058764A1 (en) 2002-12-27 2004-07-15 Bayer Healthcare Ag 4-phenyl-pyrimido [4,5-b] indole derivatives
US7172682B2 (en) 2003-01-24 2007-02-06 Rensselaer Polytechnic Institute Enzyme immobilization for electroosmotic flow
US20030224411A1 (en) 2003-03-13 2003-12-04 Stanton Lawrence W. Genes that are up- or down-regulated during differentiation of human embryonic stem cells
US7084246B2 (en) 2003-04-17 2006-08-01 Molecular Logix, Inc. Epidermal growth factor agonists
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US8586357B2 (en) 2003-12-23 2013-11-19 Viacyte, Inc. Markers of definitive endoderm
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
CA2549605C (en) 2003-12-23 2013-05-07 Cythera, Inc. Definitive endoderm
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
CN1950498A (zh) 2004-03-10 2007-04-18 加利福尼亚大学董事会 培养胚胎干细胞的组合物和方法
EA011074B1 (ru) 2004-03-30 2008-12-30 Дзе Риджентс Оф Дзе Юниверсити Оф Калифорния Гидразидсодержащие соединения - ингибиторы cftr и их применение
EP1730268A2 (en) 2004-04-01 2006-12-13 Wisconsin Alumni Research Foundation Differentiation of stem cells to endoderm and pancreatic lineage
EP2377922B1 (en) 2004-04-27 2020-04-08 Viacyte, Inc. PDX1 expressing endoderm
CA2573437C (en) 2004-07-13 2016-04-05 Geron Corporation Medium for growing human embryonic stem cells
CA2576872C (en) 2004-08-13 2013-11-12 University Of Georgia Research Foundation, Inc. Compositions and methods for self-renewal and differentiation in human embryonic stem cells
US7517870B2 (en) 2004-12-03 2009-04-14 Fondazione Telethon Use of compounds that interfere with the hedgehog signaling pathway for the manufacture of a medicament for preventing, inhibiting, and/or reversing ocular diseases related with ocular neovascularization
EP1676574A3 (en) 2004-12-30 2006-07-26 Johnson & Johnson Vision Care, Inc. Methods for promoting survival of transplanted tissues and cells
EP1859026A2 (en) 2005-01-31 2007-11-28 ES Cell International Pte Ltd. Directed differentiation of embryonic stem cells and uses thereof
GB2441718B (en) 2005-06-22 2010-10-06 Geron Corp Differentiation of human embryonic stem cells to cardiomyocyte-lineage cells
KR20160116024A (ko) 2005-06-22 2016-10-06 아스테리아스 바이오세라퓨틱스, 인크. 인간 배아 줄기 세포의 현탁 배양
WO2007011905A2 (en) 2005-07-19 2007-01-25 The University Of Tennessee Research Foundation Lpa2 receptor agonist inhibitors of cftr
CA2627645C (en) 2005-10-27 2015-07-07 Cythera, Inc. Pdx1-expressing dorsal and ventral foregut endoderm
KR20080082618A (ko) 2005-12-16 2008-09-11 알콘, 인코퍼레이티드 Ａｌｋ5 조절제를 사용한 안압의 조절
WO2007075807A2 (en) 2005-12-20 2007-07-05 Es Cell International Pte Ltd. Methods for the directed differentiation of embryonic stem cell
JP5459823B2 (ja) 2006-01-20 2014-04-02 リニューロン インコーポレイテッド 膵臓細胞培養における膵臓前駆細胞の細胞増殖促進方法
JPWO2007088651A1 (ja) 2006-02-01 2009-06-25 国立大学法人 東京大学 ＴＧＦ−βシグナル阻害剤と抗腫瘍剤の組み合せ使用
US7973011B2 (en) 2006-02-13 2011-07-05 The Board Of Trustees Of The Leland Stanford Junior University Blockade of gamma-secretase activity to promote myelination by oligodendrocytes
CA2643478C (en) 2006-02-23 2019-06-18 Novocell, Inc. Compositions and methods useful for culturing differentiable cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
CA3147112A1 (en) 2006-03-02 2007-09-13 Viacyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US8247531B2 (en) 2006-03-20 2012-08-21 Cochran Jennifer R Mutant epidermal growth factor polypeptides, nucleic acids, and uses therefor
EP2021462B1 (en) 2006-04-28 2019-01-09 Lifescan, Inc. Differentiation of human embryonic stem cells
WO2007139929A2 (en) 2006-05-25 2007-12-06 The Burnham Institute For Medical Research Methods for culture and production of single cell populations of human embryonic stem cells
CA2654196A1 (en) * 2006-06-02 2007-12-13 University Of Georgia Research Foundation, Inc. Pancreatic and liver endoderm cells and tissue by differentiation of definitive endoderm cells obtained from human embryonic stems
US8415153B2 (en) 2006-06-19 2013-04-09 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells
AU2007277364B2 (en) 2006-07-26 2010-08-12 Viacyte, Inc. Methods of producing pancreatic hormones
WO2008027420A2 (en) 2006-08-29 2008-03-06 The University Of Akron Implantable devices for producing insulin
WO2008083331A2 (en) * 2006-12-29 2008-07-10 The Regents Of The University Of Colorado Diagnostic and therapeutic target for autoimmune diseases and uses thereof
EP2121905B1 (en) * 2007-02-21 2016-02-10 Sarl Endocells Human pancreatic beta cell lines for diagnostic of diabetes
KR20100016073A (ko) 2007-04-02 2010-02-12 인스티튜트 포 원월드 헬스 Ｃｆｔｒ 억제제 화합물 및 이의 용도
CN101861386A (zh) 2007-07-18 2010-10-13 生命扫描有限公司 人胚胎干细胞的分化
KR101617243B1 (ko) 2007-07-31 2016-05-02 라이프스캔, 인코포레이티드 인간 배아 줄기 세포의 분화
JP5374370B2 (ja) 2007-08-09 2013-12-25 株式会社ヤクルト本社 新規γセクレターゼ阻害剤
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
US8623650B2 (en) 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
MX2010005805A (es) 2007-11-27 2010-06-09 Lifescan Inc Diferenciacion de celulas madre embrionarias humanas.
SG154367A1 (en) * 2008-01-31 2009-08-28 Es Cell Int Pte Ltd Method of differentiating stem cells
EP2265603B1 (en) 2008-03-13 2014-05-07 The General Hospital Corporation Inhibitors of the bmp signaling pathway
US8338170B2 (en) 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
AU2008355123B2 (en) 2008-04-21 2014-12-04 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
WO2009132083A2 (en) 2008-04-22 2009-10-29 President And Fellows Of Harvard College Compositions and methods for promoting the generation of pdx1+ pancreatic cells
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
US20090298178A1 (en) 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm
KR101651661B1 (ko) 2008-06-30 2016-08-26 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
US9683215B2 (en) * 2008-08-22 2017-06-20 President And Fellows Of Harvard College Methods of reprogramming cells
US20120021519A1 (en) 2008-09-19 2012-01-26 Presidents And Fellows Of Harvard College Efficient induction of pluripotent stem cells using small molecule compounds
US9012218B2 (en) * 2008-10-31 2015-04-21 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
PL2346988T3 (pl) 2008-10-31 2017-10-31 Janssen Biotech Inc Różnicowanie ludzkich zarodkowych komórek macierzystych do linii endokrynnej trzustki
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
EP4176888A1 (en) 2008-11-14 2023-05-10 ViaCyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
JP5719305B2 (ja) 2008-11-20 2015-05-13 ヤンセン バイオテツク，インコーポレーテツド 平面支持体上での細胞付着及び培養のための方法及び組成物
GB201111244D0 (en) * 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
US8507274B2 (en) 2009-02-06 2013-08-13 President And Fellows Of Harvard College Compositions and methods for promoting the generation of definitive endoderm
CN102695511A (zh) 2009-04-17 2012-09-26 舒玛健康系统有限责任公司 抑制眼部瘢痕形成的转化生长因子-β受体抑制剂的用途
EP2421957B1 (en) * 2009-04-22 2020-11-18 Viacyte, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
US9109245B2 (en) 2009-04-22 2015-08-18 Viacyte, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
US8685726B2 (en) 2009-04-27 2014-04-01 Viacyte, Inc. Small molecules supporting pluripotent cell growth and methods thereof
US9328331B2 (en) * 2009-05-21 2016-05-03 Joslin Diabetes Center, Inc. Compositions and methods for promoting beta cell maturity
EP2456858B1 (en) * 2009-07-20 2018-08-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
EP2456859A4 (en) 2009-07-20 2015-03-18 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
AU2010319921A1 (en) * 2009-10-29 2012-05-17 Janssen Biotech Inc. Pluripotent stem cells
CN102741395B (zh) 2009-12-23 2016-03-16 詹森生物科技公司 人胚胎干细胞的分化
JP6013196B2 (ja) * 2010-03-01 2016-10-25 ヤンセン バイオテツク，インコーポレーテツド 多能性幹細胞から誘導した細胞を精製するための方法
CN102959076B (zh) 2010-03-31 2015-09-16 斯克里普斯研究所 重编程细胞
US8927274B2 (en) 2010-04-12 2015-01-06 Technion Research & Development Foundation Limited Populations of pancreatic progenitor cells and methods of isolating and using same
EP2563908B1 (en) 2010-04-25 2019-01-09 Icahn School of Medicine at Mount Sinai Generation of anterior foregut endoderm from pluripotent cells
AU2011250912A1 (en) * 2010-05-12 2012-11-22 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
JP5875517B2 (ja) * 2010-08-09 2016-03-02 武田薬品工業株式会社 膵ホルモン産生細胞の製造法
DK2603226T3 (da) * 2010-08-12 2021-06-07 Janssen Biotech Inc Behandling af diabetes med endokrine pankreas-prækursorceller
GB201014169D0 (en) 2010-08-25 2010-10-06 Cambridge Entpr Ltd In vitro hepatic differentiation
CA2809300A1 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
EP2611907B1 (en) 2010-08-31 2016-05-04 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
AU2011296382B2 (en) 2010-08-31 2016-04-14 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
WO2012044486A1 (en) 2010-09-28 2012-04-05 Baylor Research Institute Induction of pancreatic stem cells by transient overexpression of reprogramming factors and pdxi selection
EP2508607A1 (en) 2011-04-07 2012-10-10 Helmholtz-Zentrum für Infektionsforschung GmbH Medicament for liver regeneration and for treatment of liver failure
US20120270890A1 (en) * 2011-04-22 2012-10-25 Cheng-Ho Chung Methods of Pancreatic Beta Cell Regeneration
EP2726468B1 (en) 2011-06-27 2017-04-12 Alectos Therapeutics Inc. Selective glycosidase inhibitors and uses thereof
WO2013000084A1 (en) 2011-06-27 2013-01-03 Alectos Therapeutics Inc. Selective glycosidase inhibitors and uses thereof
WO2013018851A1 (ja) 2011-08-02 2013-02-07 独立行政法人国立がん研究センター 誘導肝幹細胞から肝分化誘導する方法及び誘導肝前駆細胞
US20140243374A1 (en) 2011-10-04 2014-08-28 The Brigham And Women's Hospital, Inc. Novel Sulfonamides
DK2768948T3 (en) * 2011-10-18 2017-08-14 Univercell Biosolutions PREPARATION OF A HUMAN BETA CELL LINE FROM AN EARLY POSTNATAL ARGUMENT
JP6422778B2 (ja) 2011-11-13 2018-11-14 ブランシェット・ロックフェラー・ニューロサイエンスィズ・インスティテュート Ｐｋｃアクチベーターおよびその組合せ
SG10201608914WA (en) 2011-12-22 2016-12-29 Janssen Biotech Inc Differentiation of human embryonic stem cells into single hormonal insulin positive cells
WO2013169769A1 (en) 2012-05-07 2013-11-14 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endoderm
WO2013166654A1 (en) 2012-05-08 2013-11-14 Merck Sharp & Dohme Corp. Permeable glycosidase inhibitors and uses thereof
EP2847199B1 (en) 2012-05-08 2017-10-25 Merck Sharp & Dohme Corp. Permeable glycosidase inhibitors and uses thereof
US10066210B2 (en) 2012-06-08 2018-09-04 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells
BR112014031424A2 (pt) 2012-06-14 2017-06-27 Janssen Biotech Inc tratamento de células pluripotentes
CN104903440B (zh) 2012-09-03 2018-04-06 诺和诺德股份有限公司 使用小分子从多能干细胞产生胰内胚层
KR101942769B1 (ko) * 2012-12-31 2019-01-28 얀센 바이오테크 인코포레이티드 Hb9 조절제를 사용하는 인간 배아 줄기세포의 췌장 내분비 세포로의 분화
BR112015015714A2 (pt) 2012-12-31 2017-07-11 Janssen Biotech Inc suspensão e aglomeração de células pluripotentes humanas para diferenciação em célu-las endócrinas pancreáticas
SG10201709338RA (en) * 2012-12-31 2017-12-28 Janssen Biotech Inc Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
WO2014130887A1 (en) 2013-02-22 2014-08-28 Cedars-Sinai Medical Center Pancreatic insulin-producing beta-cell lines derived from human pluripotent stem cells
US20140287944A1 (en) 2013-02-26 2014-09-25 President And Fellows Of Harvard College Markers for functionally mature beta-cells and methods of using the same
BR112015022993B1 (pt) 2013-03-14 2021-12-14 Sumitomo Dainippon Pharma Oncology, Inc. Inibidores de jak2 e alk2, composição farmacêutica compreendendo os referidos inibidores e uso destes
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
US20140329704A1 (en) 2013-03-28 2014-11-06 President And Fellows Of Harvard College Markers for mature beta-cells and methods of using the same
KR102205234B1 (ko) 2013-06-11 2021-01-20 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 SC-β 세포 및 조성물 그리고 그 생성 방법
US20160208215A1 (en) 2013-08-30 2016-07-21 Novo Nordisk A/S Generation of Endocrine Progenitor Cells from Human Pluripotent Stem Cells Using Small Molecules
CN117821369A (zh) 2014-05-16 2024-04-05 詹森生物科技公司 小分子增强胰腺内分泌细胞中的mafa表达的用途
US10174289B2 (en) 2014-05-28 2019-01-08 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
CN106795487B (zh) 2014-08-04 2021-11-19 武田药品工业株式会社 胰腺祖细胞的增殖方法
WO2016100925A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College METHODS FOR GENERATING AUGMENTED STEM CELL-DERIVED β CELLS AND USES THEREOF
CN107614678B (zh) 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
WO2016100921A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College METHODS FOR GENERATING STEM CELL-DERIVED β CELLS AND USES THEREOF
US10443042B2 (en) 2014-12-18 2019-10-15 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
DK3234110T3 (da) 2014-12-18 2024-05-13 Harvard College FREMGANGSMÅDER TIL GENERERING AF STAMCELLE-AFLEDTE ß-CELLER OG ANVENDELSER DERAF
US9974784B2 (en) 2015-01-21 2018-05-22 The Texas A&M University System Inhibitors of activin-like receptor kinases

Also Published As

Publication number Publication date
KR20160034892A (ko) 2016-03-30
JP6893527B2 (ja) 2021-06-23
KR20200143750A (ko) 2020-12-24
RU2016100219A (ru) 2017-07-17
KR102341674B1 (ko) 2021-12-22
KR20160099079A (ko) 2016-08-19
JP6514689B2 (ja) 2019-05-15
US20240101963A1 (en) 2024-03-28
BR112015030946A2 (pt) 2017-07-25
US20170029778A1 (en) 2017-02-02
KR20210008926A (ko) 2021-01-25
KR102580225B1 (ko) 2023-09-20
WO2015002724A4 (en) 2015-03-26
DK3008168T3 (da) 2020-05-04
EP3008168A4 (en) 2017-05-31
GB2531952A (en) 2016-05-04
KR102523912B1 (ko) 2023-04-21
DE112014002780T5 (de) 2016-06-23
CN110551678A (zh) 2019-12-10
AU2023229504A1 (en) 2023-09-28
KR102201638B1 (ko) 2021-01-13
AU2020289721A1 (en) 2021-01-21
KR20230135180A (ko) 2023-09-22
MX2019003616A (es) 2019-08-01
US20230076529A1 (en) 2023-03-09
US20150240212A1 (en) 2015-08-27
US20200347358A1 (en) 2020-11-05
CN110551678B (zh) 2024-02-20
KR20210034688A (ko) 2021-03-30
KR20230054767A (ko) 2023-04-25
KR102640729B1 (ko) 2024-02-27
US11078463B2 (en) 2021-08-03
AU2020289721B2 (en) 2023-09-14
JP2024054254A (ja) 2024-04-16
BR112015030972A2 (pt) 2017-07-25
EP3008170A4 (en) 2016-11-09
CN112226402A (zh) 2021-01-15
GB2549822B (en) 2018-05-02
SG11201510176VA (en) 2016-03-30
BR112015030946A8 (pt) 2020-01-28
CA2915088A1 (en) 2015-01-08
WO2015002724A2 (en) 2015-01-08
RU2019108582A (ru) 2019-07-05
CN105579577A (zh) 2016-05-11
KR102423106B1 (ko) 2022-07-21
GB201622354D0 (en) 2017-02-15
MX2015017103A (es) 2016-11-07
GB2531952C (en) 2019-10-09
EP3569694A1 (en) 2019-11-20
US11827905B2 (en) 2023-11-28
JP2021121223A (ja) 2021-08-26
JP6677634B2 (ja) 2020-04-08
JP2016532436A (ja) 2016-10-20
JP2019141072A (ja) 2019-08-29
US11162078B2 (en) 2021-11-02
AU2014278178A1 (en) 2016-01-28
US20190169575A1 (en) 2019-06-06
US20190040362A1 (en) 2019-02-07
PH12015502767A1 (en) 2016-03-21
KR20220106230A (ko) 2022-07-28
CA2915091A1 (en) 2014-12-18
GB201521857D0 (en) 2016-01-27
JP2020108394A (ja) 2020-07-16
US10030229B2 (en) 2018-07-24
DE202014011287U1 (de) 2019-02-06
AU2014284677A1 (en) 2016-02-25
WO2014201167A4 (en) 2015-01-29
KR102631225B1 (ko) 2024-01-31
AU2020203127B2 (en) 2023-06-22
KR20240014630A (ko) 2024-02-01
US10655106B2 (en) 2020-05-19
US20220396775A1 (en) 2022-12-15
KR102232650B1 (ko) 2021-03-29
AU2020203127C1 (en) 2023-12-21
GB2549822A (en) 2017-11-01
US20220162562A1 (en) 2022-05-26
US20200385681A1 (en) 2020-12-10
EP3008168A2 (en) 2016-04-20
RU2692595C2 (ru) 2019-06-25
WO2015002724A3 (en) 2015-02-26
JP2023093654A (ja) 2023-07-04
KR20220051442A (ko) 2022-04-26
EP3008168B1 (en) 2020-02-19
KR20210156337A (ko) 2021-12-24
HK1257187B (zh) 2020-06-12
RU2016100433A (ru) 2017-07-17
AU2014284677B2 (en) 2020-03-12
KR102361924B1 (ko) 2022-02-14
JP2016531087A (ja) 2016-10-06
AU2023237028A1 (en) 2023-10-12
KR102390167B1 (ko) 2022-04-25
RU2016100219A3 (pt) 2018-03-30
US20190017031A1 (en) 2019-01-17
EP3008170A1 (en) 2016-04-20
GB2557508A (en) 2018-06-20
WO2014201167A1 (en) 2014-12-18
GB2557508A9 (en) 2018-09-05
CN106414718A (zh) 2017-02-15
SG11201510177WA (en) 2016-01-28
US11104883B2 (en) 2021-08-31
GB2531952B (en) 2017-02-08
JP7354026B2 (ja) 2023-10-02
US20240124847A1 (en) 2024-04-18
US20210079355A1 (en) 2021-03-18
GB201803318D0 (en) 2018-04-18
BR112015030972A8 (pt) 2020-01-28
KR102205234B1 (ko) 2021-01-20
US20150218522A1 (en) 2015-08-06
GB2557508B (en) 2018-11-07
AU2020203127A1 (en) 2020-06-04
AU2014278178B2 (en) 2021-01-21
RU2019108582A3 (pt) 2021-08-04
KR20220025151A (ko) 2022-03-03

Similar Documents

Publication Publication Date Title
IL273205A (en) Preparations and methods
GB201622354D0 (en) SC-� Cells and Compositions and methods for generating the same
IL267283A (en) Methods and preparations for producing epicardial cells
HK1218837A1 (zh) 組合物和方法
HK1218560A1 (zh) 組成與方法
GB2533892B (en) Compositions and methods
IL265876A (en) Preparations that include 15-ohepa and methods of using them
GB201308072D0 (en) Compositions and methods
EP2943591A4 (en) METHOD AND COMPOSITIONS FOR PROFILING SINGLE CELL EXPRESSION
GB201305813D0 (en) Compositions and methods
EP2970915A4 (en) METHOD AND COMPOSITIONS FOR PRODUCING STABILIZED TRANSFECTED CELLS
GB201322456D0 (en) Radiotracer compositions and methods
EP2964610A4 (en) VINYL SULFON-BASED 18F LABELING COMPOSITIONS AND METHOD AND USES THEREOF
SG10201802231RA (en) Methods and compositions for producing hepatocyte-like cells
SG11201604904XA (en) Detergent and rinse-aid compositions and methods
EP3001881A4 (en) PHOTOVOLTAIC DEVICES AND METHODS OF MAKING THE SAME
GB201322617D0 (en) Methods and compositions
GB201312393D0 (en) Compositions and Methods
GB201300628D0 (en) Compositions comprising 15-OHEPA and methods of using the same