SG10201601281WA - Synthetic oplophorus luciferases with enhanced light output - Google Patents
Synthetic oplophorus luciferases with enhanced light outputInfo
- Publication number
- SG10201601281WA SG10201601281WA SG10201601281WA SG10201601281WA SG10201601281WA SG 10201601281W A SG10201601281W A SG 10201601281WA SG 10201601281W A SG10201601281W A SG 10201601281WA SG 10201601281W A SG10201601281W A SG 10201601281WA SG 10201601281W A SG10201601281W A SG 10201601281WA
- Authority
- SG
- Singapore
- Prior art keywords
- light output
- enhanced light
- oplophorus luciferases
- synthetic oplophorus
- synthetic
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17483809P | 2009-05-01 | 2009-05-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10201601281WA true SG10201601281WA (en) | 2016-03-30 |
Family
ID=42358981
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201601281WA SG10201601281WA (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with enhanced light output |
| SG2011074242A SG175180A1 (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with enhanced light output |
| SG10201901453PA SG10201901453PA (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with enhanced light output |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG2011074242A SG175180A1 (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with enhanced light output |
| SG10201901453PA SG10201901453PA (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with enhanced light output |
Country Status (16)
| Country | Link |
|---|---|
| US (7) | US8557970B2 (enExample) |
| EP (5) | EP3181687B1 (enExample) |
| JP (2) | JP6038649B2 (enExample) |
| KR (1) | KR101665352B1 (enExample) |
| CN (2) | CN108192950B (enExample) |
| AU (1) | AU2010242771B2 (enExample) |
| BR (1) | BRPI1010019B1 (enExample) |
| CA (1) | CA2758572A1 (enExample) |
| DK (4) | DK3181687T3 (enExample) |
| ES (5) | ES2795287T3 (enExample) |
| IL (3) | IL215697B (enExample) |
| LT (1) | LT3409764T (enExample) |
| PL (5) | PL2990478T3 (enExample) |
| PT (4) | PT2456864E (enExample) |
| SG (3) | SG10201601281WA (enExample) |
| WO (1) | WO2010127368A1 (enExample) |
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| JP6511720B2 (ja) * | 2013-02-28 | 2019-05-15 | Jnc株式会社 | コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法 |
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| CN115161296B (zh) * | 2021-04-01 | 2024-08-13 | 深圳华大生命科学研究院 | 一种刺虾荧光素酶Nluc的突变体及其应用 |
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2010
- 2010-05-03 ES ES18184699T patent/ES2795287T3/es active Active
- 2010-05-03 US US12/773,002 patent/US8557970B2/en active Active
- 2010-05-03 PT PT107170136T patent/PT2456864E/pt unknown
- 2010-05-03 PL PL15186652T patent/PL2990478T3/pl unknown
- 2010-05-03 JP JP2012508822A patent/JP6038649B2/ja active Active
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