KR101665352B1 - 광 출력이 향상된 합성 오플로포러스 루시퍼라제 - Google Patents

광 출력이 향상된 합성 오플로포러스 루시퍼라제 Download PDF

Info

Publication number
KR101665352B1
KR101665352B1 KR1020117028749A KR20117028749A KR101665352B1 KR 101665352 B1 KR101665352 B1 KR 101665352B1 KR 1020117028749 A KR1020117028749 A KR 1020117028749A KR 20117028749 A KR20117028749 A KR 20117028749A KR 101665352 B1 KR101665352 B1 KR 101665352B1
Authority
KR
South Korea
Prior art keywords
gly
luciferase
val
polynucleotide
leu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020117028749A
Other languages
English (en)
Korean (ko)
Other versions
KR20120088548A (ko
Inventor
랜스 피. 엔셀
키스 브이. 우드
모니카 지. 우드
메리 홀
폴 오토
게디미나스 비두기리스
크리스토퍼 짐머맨
Original Assignee
프로메가 코포레이션
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 프로메가 코포레이션 filed Critical 프로메가 코포레이션
Publication of KR20120088548A publication Critical patent/KR20120088548A/ko
Application granted granted Critical
Publication of KR101665352B1 publication Critical patent/KR101665352B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020117028749A 2009-05-01 2010-05-03 광 출력이 향상된 합성 오플로포러스 루시퍼라제 Active KR101665352B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US17483809P 2009-05-01 2009-05-01
US61/174,838 2009-05-01
PCT/US2010/033449 WO2010127368A1 (en) 2009-05-01 2010-05-03 Synthetic oplophorus luciferases with enhanced light output

Publications (2)

Publication Number Publication Date
KR20120088548A KR20120088548A (ko) 2012-08-08
KR101665352B1 true KR101665352B1 (ko) 2016-10-12

Family

ID=42358981

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020117028749A Active KR101665352B1 (ko) 2009-05-01 2010-05-03 광 출력이 향상된 합성 오플로포러스 루시퍼라제

Country Status (16)

Country Link
US (7) US8557970B2 (enExample)
EP (5) EP3744834B1 (enExample)
JP (2) JP6038649B2 (enExample)
KR (1) KR101665352B1 (enExample)
CN (2) CN108192950B (enExample)
AU (1) AU2010242771B2 (enExample)
BR (1) BRPI1010019B1 (enExample)
CA (1) CA2758572A1 (enExample)
DK (4) DK2990478T3 (enExample)
ES (5) ES3042791T3 (enExample)
IL (3) IL215697B (enExample)
LT (1) LT3409764T (enExample)
PL (4) PL3409764T3 (enExample)
PT (4) PT2990478T (enExample)
SG (3) SG10201901453PA (enExample)
WO (1) WO2010127368A1 (enExample)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101665352B1 (ko) 2009-05-01 2016-10-12 프로메가 코포레이션 광 출력이 향상된 합성 오플로포러스 루시퍼라제
AU2011323418B2 (en) 2010-11-02 2017-07-27 Promega Corporation Novel coelenterazine substrates and methods of use
EP2635583B1 (en) 2010-11-02 2015-06-03 Promega Corporation Coelenterazine derivatives and methods of using same
JP5896679B2 (ja) * 2011-03-15 2016-03-30 オリンパス株式会社 オオオバボタル由来ルシフェラーゼ
EP2782916B1 (en) 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
US10519483B2 (en) 2012-02-21 2019-12-31 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
MX382157B (es) 2012-02-21 2025-03-13 Laboratory Corp America Holdings Metodos y sistemas para deteccion de microorganismos.
WO2014052653A1 (en) 2012-09-26 2014-04-03 Promega Corporation Real-time monitoring
KR102272233B1 (ko) 2012-12-12 2021-07-01 프로메가 코포레이션 생체활성 제제의 세포 표적 포획을 위한 조성물 및 방법
US10024862B2 (en) * 2012-12-12 2018-07-17 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
JP6511720B2 (ja) 2013-02-28 2019-05-15 Jnc株式会社 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法
US11072811B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
ES2926463T3 (es) 2013-03-15 2022-10-26 Promega Corp Activación de la bioluminiscencia mediante complementación estructural
US10168323B2 (en) 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
EP2978857A2 (en) 2013-03-27 2016-02-03 Sample Technologies, Inc. Recombinant phage and bacterial detection methods
JP6040846B2 (ja) 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
CA2915820A1 (en) 2013-06-19 2014-12-24 Michael Sandor Koeris Phage-based bacterial detection assay
JP6484987B2 (ja) * 2013-10-21 2019-03-20 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
JP6442825B2 (ja) 2013-12-26 2018-12-26 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
US9927430B2 (en) 2014-01-29 2018-03-27 Promega Corporation Pro-substrates for live cell applications
JP6703484B2 (ja) 2014-01-29 2020-06-03 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 細胞による取り込み測定のための、標識用試薬としての、キノンでマスクされたプローブ
JP6256118B2 (ja) * 2014-03-11 2018-01-10 Jnc株式会社 エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法
JP6265020B2 (ja) 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
US9732373B2 (en) 2014-09-11 2017-08-15 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US11365402B2 (en) 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
EP3191601B1 (en) * 2014-09-12 2022-11-02 Promega Corporation Internal protein tags
WO2016127100A1 (en) 2015-02-05 2016-08-11 Promega Corporation Luciferase-based thermal shift assays
JP6876002B2 (ja) 2015-06-05 2021-05-26 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 機能的要素を共有結合により係留させるための細胞透過性、細胞適合性、かつ開裂可能であるリンカー
EP3313846B1 (en) 2015-06-25 2020-05-06 Promega Corporation Thienopyrrole compounds and uses thereof as inhibitors of oplophorus-derived luciferases
US10316070B2 (en) 2016-09-09 2019-06-11 Promega Corporation Dual protected pro-coelenterazine substrates
US11307205B2 (en) 2016-09-19 2022-04-19 University Of Southern California Non-radioactive cytotoxicity assays
KR102556363B1 (ko) 2016-10-20 2023-07-18 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 보툴리눔 신경독소의 활성을 측정하기 위한 시험관내 및 세포 기반 검정
JP7200102B2 (ja) 2016-11-01 2023-01-06 プロメガ コーポレイション エネルギー受容体に係留されたセレンテラジン類縁体
DK3548492T5 (en) 2016-12-01 2024-08-05 Promega Corp Cell impermeable coelenterazine analogues
JP7161475B2 (ja) 2016-12-28 2022-10-26 プロメガ コーポレイション 官能化ｎａｎｏｌｕｃ阻害剤
JP7269925B2 (ja) * 2017-10-25 2023-05-09 エフ． ホフマン－ラ ロシュ アーゲー 改良された遺伝子改変／突然変異型細菌ルシフェラーゼ
US20200393446A1 (en) 2017-12-08 2020-12-17 Centre National De La Recherche Scientifique (Cnrs) Bioluminescent screening test for detecting cell cytolysis
EP3775182A1 (en) * 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides
EP3775242A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited High hydrogen utilization and gas recycle
EP3775240A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Method for controlling dissolved oxygen concentration in a continuous aerobic fermentation
WO2019213119A1 (en) 2018-05-01 2019-11-07 Promega Corporation Coelenterazine compounds as nanoluc suicide substrates
EP3788156A1 (en) 2018-05-02 2021-03-10 INVISTA Textiles (U.K.) Limited Methods for controlling limitation conditions in product biosynthesis for non-phb generating cupriavidus or ralstonia
WO2019213033A1 (en) 2018-05-02 2019-11-07 Invista North America S.A.R.L. Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-coa-tca balance in species of the genera ralstonia and cupriavidus and organisms related thereto
US11442063B2 (en) 2018-05-30 2022-09-13 Promega Corporation Broad spectrum kinase binding agents
US11390599B2 (en) 2018-06-01 2022-07-19 Promega Corporation Inhibitors of oplophorus luciferase-derived bioluminescent complexes
US10962538B2 (en) 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
CN119955899A (zh) 2018-10-03 2025-05-09 普洛麦格公司 用于稳定腔肠素及其类似物和衍生物的组合物、方法和试剂盒
JP7703446B2 (ja) 2018-11-28 2025-07-07 プロメガ コーポレイション 反応性ペプチド標識付け
JP7634479B2 (ja) 2018-12-04 2025-02-21 プロメガ コーポレイション 広域スペクトルｇｐｃｒ結合剤
JP7584433B2 (ja) 2019-03-20 2024-11-15 プロメガ コーポレイション 光親和性プローブ
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
JP2022536960A (ja) 2019-06-21 2022-08-22 ラボラトリー コーポレイション オブ アメリカ ホールディングス Ｌｉｓｔｅｒｉａの検出のための変異体バクテリオファージを生成するための方法
CN114450326B (zh) 2019-06-24 2025-02-11 普罗美加公司 用于将生物分子递送到细胞中的改性聚胺聚合物
JP7781741B2 (ja) 2019-08-26 2025-12-08 ラボラトリー コーポレイション オブ アメリカ ホールディングス 組換え繁殖欠損指標バクテリオファージを使用して微生物を検出するためのデバイスおよび方法
US11591633B2 (en) 2019-09-11 2023-02-28 Laboratory Corporation Of America Holdings Methods and systems for the rapid detection of bacteria using recombinant bacteriophage to express an indicator subunit
BR112022010153A2 (pt) 2019-11-27 2022-08-09 Promega Corp Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit
EP4073512B1 (en) 2019-12-10 2025-11-12 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
CA3168897A1 (en) 2020-01-28 2021-08-05 Freeline Therapeutics Limited Improved assay for determining neutralising antibody titre to a viral vektor
US20210363565A1 (en) 2020-05-22 2021-11-25 Promega Corporation Enhancement of kinase target engagement
US20220064696A1 (en) 2020-08-28 2022-03-03 Promega Corporation Target engagement assay for ras proteins
EP4257978A4 (en) 2020-12-25 2024-11-13 Chugai Seiyaku Kabushiki Kaisha METHOD FOR SCREENING CANDIDATE MOLECULES CAPABLE OF FORMING A COMPLEX IN CONJUNCTION WITH MULTIPLE TARGET MOLECULES
CN118703457A (zh) * 2021-04-01 2024-09-27 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
CN113025586B (zh) * 2021-04-06 2022-10-04 湖北省中医院 一种经改造的萤光素酶突变体蛋白、生物发光探针、探针组、制备方法和检测方法
US20230357266A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
JP2025515181A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 伸長表面ループ領域を有する修飾デハロゲナーゼ
US20240174992A1 (en) 2022-05-04 2024-05-30 Promega Corporation Split modified dehalogenase variants
JP2025515179A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 円順列置換デハロゲナーゼバリアント
GB202213479D0 (en) 2022-09-14 2022-10-26 Ipsen Biopharm Ltd Cell-free clostridial neurotoxin assays
WO2024059832A1 (en) * 2022-09-15 2024-03-21 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for assessing kinase activity
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay
CN119307519B (zh) * 2024-07-22 2025-11-25 苏州剂泰医药科技有限公司 编码荧光素酶的mRNA及其应用
CN119709661B (zh) * 2024-12-30 2025-10-10 佰翱得(无锡)新药开发有限公司 一种荧光素酶OLuc突变体及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995018853A1 (en) 1994-01-03 1995-07-13 Promega Corporation Mutant luciferases

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8911957D0 (en) 1989-05-24 1989-07-12 Interox Chemicals Ltd Peroxyacid manufacture
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
JP4537573B2 (ja) * 1997-09-19 2010-09-01 プロメガ・コーポレイシヨン 熱安定ルシフェラーゼと生産方法
JP3694181B2 (ja) * 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法
EP1224294B1 (en) * 1999-10-26 2007-10-03 The Secretary of State for Defence Mutant luciferase
JP4613441B2 (ja) * 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
EP1156103B1 (en) 2000-04-26 2010-08-11 Chisso Corporation Oplophorus luciferase
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
EP2433871A3 (en) 2002-06-19 2012-07-11 Medical Instill Technologies, Inc. Sterile filling machine having needle filling station within e-beam chamber
JP2008500816A (ja) * 2004-03-31 2008-01-17 ゼンコー・インコーポレイテッド 改善された性質を有するｂｍｐ−７変異体
JP3844082B2 (ja) * 2005-04-08 2006-11-08 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び耐熱性ホタルルシフェラーゼの製造法
JP4999341B2 (ja) * 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
JP5684984B2 (ja) * 2006-04-03 2015-03-18 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 順列置換及び非順列置換ルシフェラーゼバイオセンサー
WO2008118445A1 (en) 2007-03-26 2008-10-02 Promega Corporation Methods to quench light from optical reactions
EP2215227A2 (en) 2007-11-05 2010-08-11 Promega Corporation Hybrid fusion reporter and uses thereof
ATE542903T1 (de) * 2008-05-19 2012-02-15 Promega Corp Luciferase-biosensoren für camp
EP2420573A1 (en) 2009-04-17 2012-02-22 National Institute of Advanced Industrial Science And Technology Stable artificial bioluminescent enzyme having super-high brightness
KR101665352B1 (ko) 2009-05-01 2016-10-12 프로메가 코포레이션 광 출력이 향상된 합성 오플로포러스 루시퍼라제
AU2011323418B2 (en) * 2010-11-02 2017-07-27 Promega Corporation Novel coelenterazine substrates and methods of use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995018853A1 (en) 1994-01-03 1995-07-13 Promega Corporation Mutant luciferases

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Analytical Biochemistry, Vol. 366, Pages 131-136(공개일: 2007. 4. 18.)..*
FEBS Letters, Vol. 481, Pages 19-25(공개일: 2000)..*

Also Published As

Publication number Publication date
PT3181687T (pt) 2018-10-19
EP3181687A1 (en) 2017-06-21
WO2010127368A1 (en) 2010-11-04
ES2691539T3 (es) 2018-11-27
EP2990478A1 (en) 2016-03-02
PL3409764T3 (pl) 2020-07-13
IL265263B (en) 2020-05-31
JP2016028599A (ja) 2016-03-03
JP6227615B2 (ja) 2017-11-08
EP3744834A1 (en) 2020-12-02
BRPI1010019B1 (pt) 2019-02-05
PL2990478T3 (pl) 2017-08-31
PL3181687T3 (pl) 2018-11-30
EP3744834B1 (en) 2025-09-10
KR20120088548A (ko) 2012-08-08
US20190185908A1 (en) 2019-06-20
CA2758572A1 (en) 2010-11-04
JP2012525819A (ja) 2012-10-25
IL215697B (en) 2019-03-31
EP3409764A1 (en) 2018-12-05
EP2990478B1 (en) 2017-04-05
IL274145B (en) 2021-07-29
DK3181687T3 (en) 2018-09-10
US20210032676A1 (en) 2021-02-04
AU2010242771B2 (en) 2015-01-22
SG10201901453PA (en) 2019-03-28
SG10201601281WA (en) 2016-03-30
AU2010242771A1 (en) 2011-11-03
PL2456864T3 (pl) 2016-04-29
JP6038649B2 (ja) 2016-12-07
EP2456864A1 (en) 2012-05-30
PT3409764T (pt) 2020-05-29
US11365436B2 (en) 2022-06-21
US20180051318A1 (en) 2018-02-22
ES2629390T3 (es) 2017-08-09
DK2456864T3 (en) 2015-12-21
CN108192950B (zh) 2021-09-03
CN102459579B (zh) 2018-02-09
EP2456864B1 (en) 2015-11-11
IL274145A (en) 2020-06-30
US8557970B2 (en) 2013-10-15
LT3409764T (lt) 2020-06-10
US10633690B2 (en) 2020-04-28
US20220389434A1 (en) 2022-12-08
US10844422B2 (en) 2020-11-24
IL215697A0 (en) 2012-01-31
EP3181687B1 (en) 2018-08-08
CN108192950A (zh) 2018-06-22
EP3744834C0 (en) 2025-09-10
ES3042791T3 (en) 2025-11-24
DK3409764T3 (da) 2020-05-25
IL265263A (en) 2019-05-30
US20200248228A1 (en) 2020-08-06
SG175180A1 (en) 2011-11-28
US9777311B2 (en) 2017-10-03
PT2990478T (pt) 2017-05-08
US11667950B2 (en) 2023-06-06
US10233485B2 (en) 2019-03-19
US20100281552A1 (en) 2010-11-04
BRPI1010019A2 (pt) 2015-08-25
CN102459579A (zh) 2012-05-16
EP3409764B1 (en) 2020-04-15
PT2456864E (pt) 2016-01-20
ES2795287T3 (es) 2020-11-23
DK2990478T3 (en) 2017-05-15
ES2559505T3 (es) 2016-02-12
US20140120548A1 (en) 2014-05-01

Similar Documents

Publication Publication Date Title
KR101665352B1 (ko) 광 출력이 향상된 합성 오플로포러스 루시퍼라제
US8563270B2 (en) Calcium-binding photoprotein, gene encoding the same, and use thereof

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

FPAY Annual fee payment

Payment date: 20190925

Year of fee payment: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 7

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 8

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 9

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 10